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Significance of HER2 protein expression and HER2 gene amplification in colorectal adenocarcinomas 预览
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作者 Xin-Yu Wang Zhi-Xue Zheng +6 位作者 Yu Sun Yan-Hua Bai Yun-Fei Shi Li-Xin Zhou Yun-Feng Yao Ai-Wen Wu Deng-Feng Cao 《世界胃肠肿瘤学杂志:英文版(电子版)》 CAS 2019年第4期335-347,共13页
BACKGROUND Human epidermal growth factor receptor 2 (HER2) is an oncogenic driver, and a well-established therapeutic target in breast and gastric cancers. While the role of HER2 as a prognostic biomarker in colorecta... BACKGROUND Human epidermal growth factor receptor 2 (HER2) is an oncogenic driver, and a well-established therapeutic target in breast and gastric cancers. While the role of HER2 as a prognostic biomarker in colorectal adenocarcinomas (CRCs) remains uncertain, its relevance as a therapeutic target has been established. We undertook the present study to evaluate the frequency of HER2 expression in CRC and to correlate it with various clinicopathological variables. AIM To correlate HER2 protein expression and HER2 gene amplification with clinicopathological features and survival in surgically resected CRC. METHODS About 1195 consecutive surgically resected CRCs were analyzed by immunohistochemical staining (IHC) to assess HER2 protein expression, and 141 selected tumors were further evaluated by fluorescence in situ hybridization (FISH) to assess HER2 gene amplification. Follow-up information was available for 1058 patients, and using this information we investigated the prevalence of HER2 protein overexpression and gene amplification in a large series of surgically resected CRCs, and evaluated the relationship between overexpression and clinicopathological parameters and prognosis. RESULTS HER2 IHC scores of 3+, 2+, 1+, and 0 were seen in 31 (2.6%), 105 (8.8%), 475 (39.7%), and 584 (48.9%) tumors, respectively. HER2 gene amplification was seen in 24/29 tumors with an IHC score of 3+(82.8%;unreadable in 2/31), 12/102 tumors with an IHC score of 2+(11.8%;unreadable in 2/104), and 0 tumors with IHC score of 1+(0/10). HER2 gene amplification was seen in 36/1191 tumors (3.0%;unreadable in 4/1195). Among the tumors with HER2 IHC scores of 3+ and 2+, the mean percentage of tumor cells with positive IHC staining was 90%(median 100%, range 40%-100%) and 67%(median 75%, range 5%-95%), respectively (P < 0.05). Among tumors with IHC scores of 2+, those with HER2 gene amplification had a higher number of tumors cells with positive IHC staining (n = 12, mean 93%, median 95%, range 90%-95%) than those without (n = 90, mean 70%, 展开更多
关键词 Human EPIDERMAL growth factor receptor 2 IMMUNOHISTOCHEMICAL STAINING Fluorescent in situ HYBRIDIZATION Prognosis Colorectal CANCERS
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Expression of Prominin-1 in Mouse Uterus During Peri-implantation 预览
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作者 Zhang Yun Cui Yun-feng +2 位作者 Ren Jie Wu Hao Ni Hua 《东北农业大学学报:英文版》 CAS 2019年第2期41-48,共8页
To investigate the expression of Prominin-1(Prom-1)in mouse uterus during peri-implantation.In situ hybridization and immunohistochemical staining were used to detect the mRNA and protein expression level of Prom-1 in... To investigate the expression of Prominin-1(Prom-1)in mouse uterus during peri-implantation.In situ hybridization and immunohistochemical staining were used to detect the mRNA and protein expression level of Prom-1 in mice uterus in early pregnancy,pseudopregnancy,estrous cycle,treated with hormone,delayed implantation and activation models.The results showed that Prom-1 was gradually weakened in uterine luminal epithelium(LE)and glandular epithelium(GE)during days 1-4 of pregnancy.During days 5-8,Prom-1 was strongly expressed in GE,and signal of Prom-1 protein was detected in matrix and decidua around the embryo.Similar to pregnancy,Prom-1 was strongly expressed in LE and GE on the 1st day and weakly expressed on the 4th day of pseudopregnancy.In addition,Prom-1 was highly expressed in LE and GE on estrus.Expression of Prom-1 was observed in the LE and the GE of delayed-implantation uterus.In activated-implantation animal model,Prom-1 was strongly expressed in the GE.In the hormone-treated model,Prom-1 expression levels were higher in the uterus of the 17β-estradiol-treated group than those in the control group.These results indicated that Prom-1 might promote the proliferation of mouse endometrial epithelium and participate in the establishment of uterine receptivity.Meanwhile,the expression of Prom-1 was up-regulated by the 17β-estradiol,indicating that Prom-1 might involve in the process of decidua development regulated by uterine glands,and Prom-1 might play an important role in mice early pregnancy. 展开更多
关键词 mouse Prominin-1 UTERUS EMBRYO implantation
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用于生物医学的新型多孔Ti6Al4V植入物的仿生设计和3D打印
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作者 Wen-ming Peng Yun-feng Liu +6 位作者 Xian-feng Jiang Xing-tao Dong Janice Jun Dale A. Baur Jia-jie Xu Hui Pan Xu Xu 《浙江大学学报:B卷英文版》 SCIE CAS CSCD 2019年第8期647-659,共13页
目的:多孔结构植入体在骨科修复领域具有极大的应用前景.本研究提出了一种在结构与力学性能方面更贴近人体骨组织的多孔Ti6Al4V植入体.创新点:针对骨组织的结构特点,提出了"分层设计"理念,以期更好地模拟皮质骨和松质骨的结构... 目的:多孔结构植入体在骨科修复领域具有极大的应用前景.本研究提出了一种在结构与力学性能方面更贴近人体骨组织的多孔Ti6Al4V植入体.创新点:针对骨组织的结构特点,提出了"分层设计"理念,以期更好地模拟皮质骨和松质骨的结构.除了结构相似以外,这种植入体在力学性能和结构稳定性方面同样具有优势.方法:将传统多孔植入体三维晶胞设计方法转化为二维设计理念,设计出一种分层杆连接多孔结构,并利用选择性激光熔融(SLM)技术打印出样品;然后通过光学显微镜评测打印效果,利用单轴压缩试验研究分析样品的力学性能;最后利用有限元方法分析多孔结构的结构稳定性.结论:本研究所设计的新型分层杆连接结构可以通过SLM实现高质量的打印,且尺寸合理,力学性能与骨组织相接近,结构稳定性优于传统多孔结构.这种新型多孔结构植入体在骨缺损修复领域具有较好的潜在应用前景. 展开更多
关键词 分层片状杆连接多孔结构(LSRCMS) 多孔Ti6Al4V植入体 骨缺损修复 选择性激光熔融(SLM) 机械性能 有限元分析
Application of pulse index continuous cardiac output system in elderly patients with acute myocardial infarction complicated by cardiogenic shock: A prospective randomized study 预览
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作者 Yuan-Bo Zhang Zhi-Zhong Zhang +6 位作者 Jun-Xia Li Yu-Hong Wang Wei-Lin Zhang Xin-Li Tian Yun-Feng Han Meng Yang Yu Liu 《世界临床病例杂志》 2019年第11期1291-1301,共11页
BACKGROUND Cardiogenic shock (CS) secondary to acute myocardial infarction (AMI) complicates management of the condition, and often leads to poor prognosis. Prompt and accurate monitoring of cardiovascular and accompa... BACKGROUND Cardiogenic shock (CS) secondary to acute myocardial infarction (AMI) complicates management of the condition, and often leads to poor prognosis. Prompt and accurate monitoring of cardiovascular and accompanying hemodynamic changes is crucial in achieving adequate management of the condition. Advances in technology has availed procedures such as pulse index continuous cardiac output (PiCCO), which can offer precise monitoring of cardiovascular functions and hemodynamic parameters. In this study, PiCCO is evaluated for its potential utility in improving management and clinical outcomes among elderly patients with AMI complicated by CS. AIM To assess whether use of the PiCCO system can improve clinical outcomes in elderly patients with AMI complicated by CS.METHODS Patients from emergency intensive care units (EICU) or coronary care units (CCU) were randomized to receive PiCCO monitoring or not. The APACHE II score, SOFA score, hs-TnI, NT-proBNP, PaO2/FiO2 ratio and lactate levels on day 1, 3 and 7 after treatment were compared. The infusion and urine volume at 0-24 h, 24-48 h and 48-72 h were recorded, as were the cardiac index (CI), extravascular lung water index (EVLWI), intrathoracic blood volume index (ITBVI) and global end diastolic volume index (GEDVI) at similar time intervals. RESULTS Sixty patients with AMI complicated by CS were included in the study. The PiCCO group had a significantly lower APACHE II score, SOFA score, hs-TnI and NT-proBNP levels on day 1, 3 and 7 after treatment. The infusion and urine volume during 0-24 h in the PiCCO group were significantly greater, and this group also showed significantly higher ADL scores. Furthermore, the PiCCO group spent lesser days on vasoactive agents, mechanical ventilation, and had a reduced length of stay in EICU/CCU. Additionally, the CI was significantly higher at 48 h and 72 h in the PiCCO group compared with that at 24 h, and the EVLWI, ITBVI and GEDVI were significantly decreased at 48 h and 72 h. CONCLUSION Applying the PiCCO system could 展开更多
关键词 PULSE INDEX CONTINUOUS cardiac output Elderly patients CARDIOGENIC shock Acute myocardial INFARCTION
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Value of fasting plasma glucose to screen gestational diabetes mellitus before the 24th gestational week in women with different pre-pregnancy body mass index
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作者 Yu-Mei Wei Xin-Yue Liu +9 位作者 Chong Shou Xing-Hui Liu Wen-Ying Meng Zi-Lian Wang Yun-Feng Wang Yong-Qing Wang Zhen-Yu Cai Li-Xin Shang Ying Sun Hui-Xia Yang 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第8期883-888,共6页
Background:Gestational diabetes mellitus(GDM)is usually diagnosed between 24th and 28th gestational week using the 75-g oral glucose tolerance test(OGTT).It is difficult to predict GDM before 24th gestational week bec... Background:Gestational diabetes mellitus(GDM)is usually diagnosed between 24th and 28th gestational week using the 75-g oral glucose tolerance test(OGTT).It is difficult to predict GDM before 24th gestational week because fast plasma glucose(FPG)decreases as the gestational age increases.It is controversial that if FPG≥5.1 mmol/L before 24th gestational week should be intervened or not.The aim of this study was to evaluate the value of FPG to screen GDM before 24th gestational week in women with different pre-pregnancy body mass index(BMI).Methods:This was a multi-region retrospective cohort study in China.Women who had a singleton live birth between June 20,2013 and November 30,2014,resided in Beijing,Guangzhou and Chengdu,and received prenatal care in 21 selected hospitals,were included in this study.Pre-pregnancy BMI,FPG before the 24th gestational week,and one-step GDM screening with 75 g-OGTT at the 24th to 28th gestational weeks were extracted from medical charts and analyzed.The pregnant women were classified into four groups based on pre-pregnancy BMI:Group A(underweight,BMI<18.5 kg/m^2),Group B(normal,BMI 18.5-23.9 kg/m^2),Group C(overweight,BMI 24.0-27.9 kg/m^2)and Group D(obesity,BMI≥28.0 kg/m^2).The trend of FPG before 24th week of gestation was described,and the sensitivity and specificity of using FPG before the 24th gestational week to diagnose GDM among different pre-pregnancy BMI groups were reported.Differences in the means between groups were evaluated using independent sample t-test and analysis of variance.Pearson Chi-square test was used for categorical variables.Results:The prevalence of GDM was 20.0%(6806/34,087)in the study population.FPG decreased gradually as the gestational age increased in all pre-pregnancy BMI groups until the 19th gestational week.FPG was higher in women with higher pre-pregnancy BMI.FPG before the 24th gestational week and pre-pregnancy BMI could be used to predict GDM.The incidence of GDM in women with FPG≥5.10 mmol/L in the 19th to 24th gestational weeks an 展开更多
关键词 Body mass index FASTING plasma GLUCOSE GESTATIONAL diabetes MELLITUS
Therapeutic plasma exchange and continuous renal replacement therapy for severe hyperthyroidism and multi-organ failure: A case report 预览
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作者 Jun-Hui Ba Ben-Quan Wu +1 位作者 Yan-Hong Wang Yun-Feng Shi 《世界临床病例杂志》 2019年第4期500-507,共8页
BACKGROUND Severe hyperthyroidism is a life-threatening exacerbation of thyrotoxicosis, characterized by high fever and multiorgan failure. The most common medical treatments are administration of antithyroid drugs an... BACKGROUND Severe hyperthyroidism is a life-threatening exacerbation of thyrotoxicosis, characterized by high fever and multiorgan failure. The most common medical treatments are administration of antithyroid drugs and radioactive iodine, and thyroidectomy. In some patients, antithyroid therapy is limited due to serious adverse effects or failure to control disease progression. In some extreme cases, such as thyroid storm, conventional therapy alone does not yield effective and rapid improvement before the development of multiorgan failure. CASE SUMMARY This report describes a Chinese patient with severe hyperthyroidism accompanied by multiorgan failure, who was transferred to the medical intensive care unit of our hospital. The patient presented with palpitations, vomiting, diarrhea, and shortness of breath for a week. Laboratory tests showed elevation of thyroid hormones. Hepatic failure occurred with high aminotransferase levels and jaundice. Given her abnormal liver function and medication history, we could not exclude diagnosis of propylthiouracil-induced hepatic failure. Moreover, she also suffered from heart failure. Therapeutic plasma exchange (commonly known as TPE) and continuous renal replacement therapy (commonly known as CRRT) were used as life-saving therapy, which resulted in notable improvement of clinical symptoms and laboratory tests. CONCLUSION Combined TPE and CRRT are safe and effective for patients with hyperthyroidism and multiorgan failure. 展开更多
关键词 SEVERE HYPERTHYROIDISM Propylthiouracil-induced HEPATOTOXICITY Multiorgan
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A rare case of primary well-differentiated angiosarcoma of the right atrium 预览
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作者 Wei-Zi SUN Qiu-Ji WU +4 位作者 You WANG Min CHEN Jun ZHANG Gong ZHANG Yun-Feng ZHOU 《老年心脏病学杂志:英文版》 SCIE CAS CSCD 2019年第2期164-167,共4页
Primary cardiac angiosarcoma is rare in clinic.However,it is the most common malignant tumor in the heart,accounting for 15%-35%of all cardiac tumors.It is a very aggressive disease characterized by high rates of loca... Primary cardiac angiosarcoma is rare in clinic.However,it is the most common malignant tumor in the heart,accounting for 15%-35%of all cardiac tumors.It is a very aggressive disease characterized by high rates of local recurrence and systemic metastases,and it is a poor prognosis.Echocardiography,computed tomography(CT)and magnetic resonance imaging(MRI)are important tools for diagnosing cardiac angiosarcoma and are valuable in guiding surgical resection and in monitoring treatment efficacy. 展开更多
关键词 ANGIOSARCOMA CARDIAC TUMOR CHEMORADIOTHERAPY Literature review TUMOR RESECTION
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Identification and QTL mapping of Z550, a rice backcrossed inbred line with increased grains per panicle 预览
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作者 WANG Shi-ming CUI Guo-qing +8 位作者 WANG Hui MA Fu-ying XIA Sai-sai LI Yun-feng YANG Zheng-lin LING Ying-hua ZHANG Chang-wei HE Guang-hua ZHAO Fang-ming 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第3期526-531,共6页
An elite backcrossed inbred line Z550 with increased grains per panicle was identified from advanced backcrosses between Nipponbare and Xihui 18 by simple sequence repeat(SSR) marker-assisted selection(MAS). Z550 carr... An elite backcrossed inbred line Z550 with increased grains per panicle was identified from advanced backcrosses between Nipponbare and Xihui 18 by simple sequence repeat(SSR) marker-assisted selection(MAS). Z550 carries 13 substitution segments distributed on chromosomes 1, 6, 7, 8, 9, 10, and 12, with an average substitution length of 1.68 Mb. Compared with the Nipponbare parental line, plant height, panicle length, spikelets per panicle, grains per panicle, and grain weight for Z550 were significantly increased. While the grain width of Z550 was significantly narrower, and the seed setting ratio(81.43%) was significantly lower than that of Nipponbare, it is still sufficient for breeding purposes. Quantitative trait loci(QTLs) mapping for important agronomic traits was conducted with the F2 population derived from Nipponbare crossed with Z550 using the restricted maximum likelihood(REML) method. A total of 16, including 12 previously unreported QTLs were detected, with contribution rates ranging from 1.46 to 10.49%. Grains per panicle was controlled by 8 QTLs, 5 of which increased number of grains whereas 3 decreased it. qGPP-1, with the largest contribution(10.49%), was estimated to increase grains per panicle by 30.67, while q GPP-9, with the minimum contribution rate(2.47%), had an effect of increasing grains per panicle by 15.79. These results will be useful for further development of single segment substitution lines with major QTLs, and for research of their molecular functions via QTL cloning. 展开更多
关键词 RICE backcrossed INBRED line YIELD QTL MAPPING
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Value of Adjuvant Radiotherapy for Thymoma with Myasthenia Gravis after Extended Thymectomy
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作者 Chang-Feng Lu Lei YU +2 位作者 Yun Jing Yun-Feng Zhang Ji Ke 《中华医学杂志:英文版》 SCIE CAS CSCD 2018年第8期927-932,共6页
关键词 重症肌无力 放射疗法 放射治疗 价值 CSR 亚群 外科 手术
An algorithm to resolve γ-rays from charged cosmic rays with DAMPE
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作者 Zun-Lei Xu Kai-Kai Duan +18 位作者 Zhao-Qiang Shen Shi-Jun Lei Tie-Kuang Dong Fabio Gargano Simone Garrappa Dong-Ya Guo Wei Jiang Xiang Li Yun-Feng Liang Mario Nicola Mazziotta Maria Fernanda Munoz Salinas Meng Su Valerio Vagelli Qiang Yuan Chuan Yue Jing-Jing Zang Ya-Peng Zhang Yun-Long Zhang Stephan Zimmer 《天文和天体物理学研究:英文版》 CSCD 2018年第3期25-36,共12页
关键词 光线 宇宙 GEMINGA 蒙特卡罗模拟 算法 数据识别 时间分辨率 天体物理学
Simultaneous observation of quantum contextuality and quantum nonlocality
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作者 Xiao-Min Hu Bi-Heng Liu +5 位作者 Jiang-Shan Chen Yu Guo Yu-Chun Wu Yun-Feng Huang Chuan-Feng Li Guang-Can Guo 《科学通报:英文版》 SCIE EI CSCD 2018年第17期1092-1095,共4页
关键词 量子 非局域性 理论研究 物理学
有限元方法分析具有不同形态第三磨牙的下颌骨受到冲击力时的应力分布
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作者 Yun-feng LIU Russell WANG +1 位作者 Dale A.BAUR Xian-feng JIANG 《浙江大学学报:B卷英文版》 SCIE CAS CSCD 2018年第1期38-48,共11页
目的:评估下颌骨在具有不同形态的第三磨牙或者没有第三磨牙的情况下,当遭受到前部或侧部2000 N冲击力时,其应力分布。创新点:第三磨牙的存在对下颌骨的力学性能有影响,而且不同位置形态的第三磨牙对下颌骨的力学性能的影响存在差异... 目的:评估下颌骨在具有不同形态的第三磨牙或者没有第三磨牙的情况下,当遭受到前部或侧部2000 N冲击力时,其应力分布。创新点:第三磨牙的存在对下颌骨的力学性能有影响,而且不同位置形态的第三磨牙对下颌骨的力学性能的影响存在差异。方法:根据一个具有完整牙列的健康下颌骨的计算机断层扫描(CT)图像构建出其三维模型,以CT图像上的Hounsfield值(HU)为基础,计算出下颌骨的力学性能参数(包括密度和杨氏模量),共分成9组数据。构建出第三磨牙分别为水平向、垂直向以及近中方向呈45度角时的完全阻生和部分阻生的共6组下颌骨计算模型。并以无第三磨牙的下颌骨为基准模型,利用有限元方法计算在下颌前部和侧面分别受到2000 N的静态冲击力的情况下Von Mises应力分布。结论:有限元分析结果显示,相同载荷条件下,当第三磨牙部分阻生时的下颌骨髁突颈和角部区域的应力值比完全阻生时要大,因此具有部分阻生第三磨牙的下颌骨具有更高的骨折风险;当具有水平向部分阻生的第三磨牙时,下颌骨骨折的风险最大。对于各种计算模型,下颌骨受到侧向冲击力时,应力最大位置均位于下颌角区;对于无第三磨牙的下颌骨,应力最大位置位于髁突颈部区域,此时髁突颈更容易发生骨折。 展开更多
关键词 有限元分析 第三磨牙 下颌骨 生物力学仿真
Translocator protein ligand, YL-IPA08, attenuates lipopolysaccharide-induced depression-like behavior by promoting neural regeneration 预览
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作者 Xiao-Ying Zhang Li-Ming Zhang +1 位作者 Wei-Dong Mi Yun-Feng Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2018年第11期1937-1944,共8页
关键词 蛋白质 神经原 行为 稀释 新生 BRDU 调查结果 老鼠
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Trans-anal minimally invasive surgery for rectal neoplasia:Experience from single tertiary institution in China 预览
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作者 Nan Chen Yi-Fan Peng +1 位作者 Yun-Feng Yao Jin Gu 《世界胃肠肿瘤学杂志:英文版(电子版)》 CAS 2018年第6期137-144,共8页
AIM To evaluate the feasibility and safety of trans-anal minimally invasive surgery(TAMIS)from single institute in China.METHODS A retrospective review was conducted for patients with rectal neoplasia,who underwent TA... AIM To evaluate the feasibility and safety of trans-anal minimally invasive surgery(TAMIS)from single institute in China.METHODS A retrospective review was conducted for patients with rectal neoplasia,who underwent TAMIS using single incision laparoscopic surgery-Port from January 2013 till January 2016 by a group of colorectal surgeons from Gastrointestinal Center UnitⅢ,Peking University Cancer Hospital.Patients’demographic data,surgical related information,post-operational pathology,as well as perioperative follow-up were all collected.RESULTS Twenty-five patients with rectal neoplasia were identified consequently.Complete full-thickness excision was achieved in all cases without conversion.22(88%)cases had rectal malignancies[6 were adenocarcinomas and 16 were neuroendocrine tumors(NET)],while 3 patients had adenomas.Mean surgical duration was 61.3 min,and mean post-operative stay were 2.7 d.Post-operational examination demonstrated 5 cases had positive resection margin:2 adenocarcinoma cases and 1 NET case with positive lateral margin,and the other 2 NET cases with positive basal margin.The curve of operation time for TAMIS cases suggested a minimum of 10 cases for a laparoscopic surgeon proficient with this technique.CONCLUSION TAMIS was demonstrated to be reproducible and safe,with a relatively short learning process for laparoscopic surgeons in selected cases for rectal neoplasia.Longterm oncological outcome needs to be determined by further investigation. 展开更多
关键词 RECTAL NEOPLASIA Resection margin TRANSANAL MINIMALLY INVASIVE surgery
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Rainfall and inflow effects on soil erosion for hillslopes dominated by sheet erosion or rill erosion in the Chinese Mollisol region
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作者 SHEN Hai-ou WEN Lei-lei +4 位作者 HE Yun-feng HU Wei LI Hong-li CHE Xiao-cui LI Xin 《山地科学学报:英文版》 SCIE CSCD 2018年第10期2182-2191,共10页
关键词 土壤侵蚀 小河 降雨 中国 土壤损失 土壤表面 相对稳定 代理人
S1-2 The 5-HT6 Receptor-Related Mechanism for the Cognition-Enhancing Properties of Hypidone Hydrochloride(YL-0919),A Novel Protential Antidepressant 预览
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作者 JIN Zeng-liang CHEN Xiao-fei +1 位作者 ZHANG Li-ming LI Yun-feng 《神经药理学报》 2018年第4期3-4,共2页
Hypidone hydrochloride(YL-0919),the 5-HT1A/6 agonists and 5-HT reuptake inhibitor,is a novel potent antidepressant with original chemical structure.Previous studies confirmed that YL-0919 has significant antidepressan... Hypidone hydrochloride(YL-0919),the 5-HT1A/6 agonists and 5-HT reuptake inhibitor,is a novel potent antidepressant with original chemical structure.Previous studies confirmed that YL-0919 has significant antidepressant-and anxiolytic-like effects.Compared with first-line antidepressants,YL-0919 possesses rapid-onset and cognition-enhancing advantages without causing sexual disorders.Recently,it has been found that it has high affinity with 5-HT6 receptor.Objective:To study the target characteristics of YL-0919 to 5-HT6 receptors,and to explore the relationship between the 5-HT6 receptor and the cognition-enhancing,antidepressant/anxiolytic-like effects of YL-0919 and targeting mechanisms.Methods:The radioligand binding inhibition test and[35S]-GTPγS binding assay were used to evaluate the binding affinity of YL-0919 to 5-HT6 receptor in rat striatum,transient CHO cell line and stable Hela cell lines.Novel object recognition(NOR),Morris water maze(MWM)and step-down test(SD)were used to evaluate the cognition-enhancing activity of YL-0919,and the selective 5-HT6 receptor antagonist SB271046 was used to evaluate the relationship between behavioral improvement caused by YL-0919 and 5-HT6 receptor activation.To study the 5-HT6 receptor related mechanisms of YL-0919,the competitive immunofluorescence assay were used to examine the cAMP level in h5-HT6 receptor-expressed in the Hela cells Results:①Radioligand competitive binding experiments showed that YL-0919 had high binding affinity with 5-HT6 receptors in the rat striatum,the CHO cells transiently expressed the h5-HT6 receptor and the Hela cells stably expressed the h5-HT6 receptor,with Ki of 10.72,14.76 and 28.12 nM respectively;[35S]-GTPγS showed full agonist characteristics of YL-0919 in striatum and cells,with EC50 of 71.23,64.73 and 52.92 nM respectively,and the maximum efficiency(Emax)reached 100%which is the same to the 5-HT6 receptor agonist WAY208466,suggesting that YL-0919 is a full 5-HT6 receptor agonist.②Cognitive-related behavioral tests showed th 展开更多
关键词 hypidone hydrochloride(YL-0919) 5-HT6 RECEPTOR AGONIST cognitionenhancing ANTIDEPRESSANT ANXIOLYTIC cAMP
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Autoimmune liver disease-related autoantibodies in patients with biliary atresia 预览
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作者 Shu-Yin Pang Yu-Mei Dai +9 位作者 Rui-Zhong Zhang Yi-Hao Chen Xiao-Fang Peng Jie Fu Zheng-Rong Chen Yun-Feng Liu Li-Yuan Yang Zhe Wen Jia-Kang Yu Hai-Ying Liu 《世界胃肠病学杂志:英文版》 SCIE CAS 2018年第3期387-396,共10页
AIM To investigate the prevalence and clinical significance of autoimmune liver disease(ALD)-related autoantibodies in patients with biliary atresia(BA).METHODS Sera of 124 BA patients and 140 age-matched non-BA contr... AIM To investigate the prevalence and clinical significance of autoimmune liver disease(ALD)-related autoantibodies in patients with biliary atresia(BA).METHODS Sera of 124 BA patients and 140 age-matched non-BA controls were assayed for detection of the following autoantibodies:ALD profile and specific anti-nuclear antibodies(ANAs),by line-blot assay;ANA and antineutrophil cytoplasmic antibody(ANCA),by indirect immunofluorescence assay;specific ANCAs and anti-M2-3E,by enzyme linked immunosorbent assay.Associations of these autoantibodies with the clinical features of BA(i.e.,cytomegalovirus infection,degree of liver fibrosis,and short-term prognosis of Kasai procedure)were evaluated by Spearman’s correlation coefficient.RESULTS The overall positive rate of serum autoantibodies in preoperative BA patients was 56.5%.ALD profile assay showed that the positive reaction to primary biliary cholangitis-related autoantibodies in BA patients was higher than that to autoimmune hepatitis-related autoantibodies.Among these autoantibodies,anti-BPO was detected more frequently in the BA patients than in the controls(14.8%vs 2.2%,P<0.05).Accordingly,32(25.8%)of the 124 BA patients also showed a high positive reaction for anti-M2-3E.By comparison,the controls had a remarkably lower frequency of anti-M2-3E(P<0.05),with 6/92(8.6%)of patients with other liver diseases and 2/48(4.2%)of healthy controls.The prevalence of ANA in BA patients was 11.3%,which was higher than that in disease controls(3.3%,P<0.05),but the reactivity to specific ANAs was only 8.2%.The prevalence of ANCAs(ANCA or specific ANCAs)in BA patients was also remarkably higher than that in the healthy controls(37.9%vs 6.3%,P<0.05),but showed no difference from that in patients with other cholestasis.ANCA positivity was closely associated with the occurrence of postoperative cholangitis(r=0.61,P<0.05),whereas none of the autoantibodies showed a correlation to cytomegalovirus infection or the stages of liver fibrosis.CONCLUSION High prevalence of autoantibodies in 展开更多
关键词 BILIARY ATRESIA Anti-nuclear ANTIBODY Antineutrophilic CYTOPLASMIC ANTIBODY AUTOIMMUNE liver diseases AUTOANTIBODIES
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Spontaneous T-symmetry breaking and exceptional points in cavity quantum electrodynamics systems
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作者 Yu-Kun Lu Pai Peng +4 位作者 Qi-Tao Cao Da Xu Jan wiersig Qihuang Gong Yun-Feng Xiao 《科学通报:英文版》 SCIE EI CSCD 2018年第17期1096-1100,共5页
关键词 对称 系统 HAMILTONIAN 现代物理 模式控制 空间划分 原子 振荡器
S2B-3 The Translocator Protein(18 kDa),A Potential Novel Drug Target of Mental Disorders 预览
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作者 LI Yun-feng 《神经药理学报》 2018年第4期45-46,共2页
Background:Recently,the translocator protein(18 kDa)(TSPO),previously called peripheral benzodiazepine receptor(PBR),has received increased attention in the pathophysiology of several kinds of neuropsychiatric disorde... Background:Recently,the translocator protein(18 kDa)(TSPO),previously called peripheral benzodiazepine receptor(PBR),has received increased attention in the pathophysiology of several kinds of neuropsychiatric disorders.The aim of the present study was to evaluate the exact role of TSPO in the pathophysiology and treatment of mental disorders.Methods:Firstly,by using TSPO WT/KO mice or the lentiviral vectors mediating TPSO overexpression,we studied the important role of TSPO in the anxiolytic and antidepressant effects.The results obtained in this studies provided new insights into the potential target of TSPO for the treatment of mental disorders.Secondly,we determined the the target profile of YLIPA08,a potent and selective TSPO ligand synthesized by our institute.And we then measured its anti-PTSD,antidepressant and anxiolytic-like effects in various mouse and rat models.Finally,we investigated its potential to cause side effects that are typically associated with conventional benzodiazepines.Results:Our results are the first to suggest that AC-5216,a selective TSPO ligand,produce antidepressant-like and cognitive enhancing behaviors in TSPO+/+mice,but not in TSPO-/-mice.The overexpression of TSPO in the hippocampal dentate gyrus produced anxiolytic and antidepressant-like behavioural effects that are at least partially mediated by downstream allopregnanolone biosynthesis.We also showed that TSPO ligand YL-IPA08 caused significant anti-PTSD,antidepressant and anxiolytic-like effects,but does not cause the side effects that are typically associated with conventional BZDs benzodiazepines,which might be partially mediated by binding to TSPO and the subsequent synthesis of allopregnanolone.Conclusion:These data suggest that TSPO may be the promising targets for the development of TSPO ligands as the novel and promising pharmacotherapies target for the mental disorders. 展开更多
关键词 kDa TREATMENT effects EXACT MOUSE MICE its HAS
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Mouse strain differences in selective serotonin reuptake inhibitors sensitivity correlates with serotonin transporter binding and function
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作者 JIN Zeng-liang CHEN Xiao-fei +4 位作者 LI Xiao-rong XIONG Jie ZHENG Yuan-yuan GAO Na-na LI Yun-feng 《中国药理学与毒理学杂志》 CSCD 北大核心 2018年第9期710-711,共2页
OBJECTIVE Selective serotonin reuptake inhibitors (SSRIs) bind 5-HT transporters, leading to the accumulation of 5-HT and amelioration of depression. Although different mouse strain showed different sensitivity to SSR... OBJECTIVE Selective serotonin reuptake inhibitors (SSRIs) bind 5-HT transporters, leading to the accumulation of 5-HT and amelioration of depression. Although different mouse strain showed different sensitivity to SSRIs in mouse models of depression, the reason for these strain differences remains unclear. Here, therefore, in the present study, we examined immobility time and locomotor activity in two mouse strains, namely, C57BL / 6J and DBA / 2J mice, and the effects of the SSRIs fluoxetine. Furthermore, we analyzed 5-HT transporter binding and reuptake inhibition in both strains to explore their relationship with the immobility and locomotor activity effects of the three SSRIs in these two mouse strains. METHODS Strain differences in SSRI effects in the tail suspension test (TST) and forced swimming test (FST). To initiate our studies, we sought to confirm that SERT strain variation did not alter SERT protein expression, 5-HT recognition, or uptake activity when expressed in C57BL/6J and DBA/2J mice. Radioligand binding assays were conducted to determine the affinity of the SSRIs for the 5-HT transporters in the two mouse strains. RESULTS SSRI citalopram dose-dependently reduced immobility time in both the FST and TST in DBA/2J but not C57BL/6J mouse strains, whereas fluoxetine showed opposite results. Paroxetine reduced immobility time similarly in both strains. The affinity of citalopram for the 5-HT transporter in DBA/2J mice was 700-fold higher than that for in C57BL/ 6J mice, whereas the affinity of fluoxetine in C57BL/6J mice was 100-fold higher than that in the DBA/2J mouse. Furthermore, High citalopram concentrations were required to [3H]5-HT uptake in C57BL/6J but not DBA/2J mouse cortical synaptosomes, whereas fluoxetine also showed opposite results. CONCLUSION Immobility duration depends on 5-HT transporter binding levels, leading to apparent strain differences in immobility time in FST and TST. Furthermore, differences in 5-HT transporter binding may cause variations in SSRI responses on behaviors. 展开更多
关键词 antidepressants mouse STRAINS selective SEROTONIN REUPTAKE INHIBITORS
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