Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings,and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation.In this study,the d...Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings,and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation.In this study,the differences in the expression levels of 3 invertase genes,CWI,SAI and NI,between virusfree and ordinary sugarcane seedlings were analyzed.Compared with ordinary sugarcane plants,the expression of CWI was mainly up-regulated in immature leaves and stems at elongation stage and leaves and immature internodes at maturation stage,and especially,greatly up-regulated in immature internodes at maturation stage of virus-free plants.The expression of SAI and NI were mainly up-regulated in leaves and immature internodes of virus-free plants at maturation stage,which might be beneficial to sugar accumulation and rapid utilization of monosaccharide in the stalks of virus-free plants.It is further indicated that virus-free treatment could significantly improve the expression of sucrose invertases at late growth period,and might facilitate the increase of plant biomass.展开更多
<正> 有这么一则笑话:一位对自己容貌非常自信的美丽女士写信向萧伯纳求婚,说:'如果以后我们的孩子兼有我的长相、你的智慧,那将是多么完美的组合!'萧伯纳回信说:'如果孩子继承了我的容貌、你的智慧,怎么办?'也许...<正> 有这么一则笑话:一位对自己容貌非常自信的美丽女士写信向萧伯纳求婚,说:'如果以后我们的孩子兼有我的长相、你的智慧,那将是多么完美的组合!'萧伯纳回信说:'如果孩子继承了我的容貌、你的智慧,怎么办?'也许,这不仅仅是则笑话。因为我们确想知道,智慧能否遗传,或者说,能否通过增加或改变基因的组成结构,使我们的后代更聪明?这也是千百年来许多科学家研究的课题。基因是生物遗传物质的最小功能单位,就像物质的化学性质是由分子结构决定一样,生物的遗传特征由基因决定。除了少数病毒的基因由核糖核酸(RNA)组成外,多数生物的基因由脱氧核糖核酸(DNA)构成。人类共有10万条基因,基因中 DNA 的排列次序就是蛋白质合成的遗传密码,即生物的基因指令,不仅决定了我们头发与眼睛的颜色、身高、相貌,也决定了展开更多
Although the somatic cell nuclear transfer(SCNT) technique has been used extensively for cloning and generating transgenic pigs,the cloning efficiency is still very low.It has been proposed that the low efficiency of ...Although the somatic cell nuclear transfer(SCNT) technique has been used extensively for cloning and generating transgenic pigs,the cloning efficiency is still very low.It has been proposed that the low efficiency of this technique is the result of incomplete epigenetic reprogramming and abnormal gene expression during early embryonic development.In this study,we investigate the effect of Scriptaid,a low-toxicity histone deacetylase inhibitor,on the developmental competence of porcine SCNT embryos.We found that treating SCNT embryos with 500 nmol/L Scriptaid for 15 h after activation significantly enhanced the blastocyst formation rate(27.7%) compared with the untreated group(control)(12.2%,P<0.05).Using an immunofluorescence technique to measure the average fluorescence intensity,we also found that treating SCNT embryos with Scriptaid increased the level of histone acetylation on histone H3 at lysine 14(acH3K14).Furthermore,treating embryos with Scriptaid increased the expression level of three genes that play important roles during embryonic development(Oct4,Klf4 at the blastocyst stage and Nanog at the 4-cell stage).Moreover,the expression level of the apoptosis-related gene Caspase-3 was significantly lower in the Scriptaid-treated SCNT embryos compared with the control SCNT embryos at the 4-cell and blastocyst stages.In conclusion,these results indicate that Scriptaid treatment improves the development and nuclear reprogramming of porcine SCNT embryos.展开更多
基金Supported by "863" Program (2013AA102604-1) Natural Science Foundation of Hainan Province (20163124) +1 种基金 Basal Research Fund for Central Public-inter- est Scientific Institute ( ITBB140503 ) Earmarked Fund for China Agriculture Research System (CARS-20-2-5).
文摘Virus-free sugarcane seedlings have improved biomass and sucrose content compared with ordinary seedlings,and sucrose invertases are key enzymes regulating sugarcane growth and sucrose accumulation.In this study,the differences in the expression levels of 3 invertase genes,CWI,SAI and NI,between virusfree and ordinary sugarcane seedlings were analyzed.Compared with ordinary sugarcane plants,the expression of CWI was mainly up-regulated in immature leaves and stems at elongation stage and leaves and immature internodes at maturation stage,and especially,greatly up-regulated in immature internodes at maturation stage of virus-free plants.The expression of SAI and NI were mainly up-regulated in leaves and immature internodes of virus-free plants at maturation stage,which might be beneficial to sugar accumulation and rapid utilization of monosaccharide in the stalks of virus-free plants.It is further indicated that virus-free treatment could significantly improve the expression of sucrose invertases at late growth period,and might facilitate the increase of plant biomass.
基金This work was supported by a grant from the National Natural Science Foundation of China (No. 30672193).Acknowledgements: We are grateful to the patients and their families for the cooperation, to the members of the Paediatric Heart Centre for support and staff of the Central Laboratory of Molecular Biology for the technical assistance. We specially thank Dr. ZHU Xiao-quan for the excellent advice and assistance and Dr. ZHANG Wei-min for helpful patient information.
文摘<正> 有这么一则笑话:一位对自己容貌非常自信的美丽女士写信向萧伯纳求婚,说:'如果以后我们的孩子兼有我的长相、你的智慧,那将是多么完美的组合!'萧伯纳回信说:'如果孩子继承了我的容貌、你的智慧,怎么办?'也许,这不仅仅是则笑话。因为我们确想知道,智慧能否遗传,或者说,能否通过增加或改变基因的组成结构,使我们的后代更聪明?这也是千百年来许多科学家研究的课题。基因是生物遗传物质的最小功能单位,就像物质的化学性质是由分子结构决定一样,生物的遗传特征由基因决定。除了少数病毒的基因由核糖核酸(RNA)组成外,多数生物的基因由脱氧核糖核酸(DNA)构成。人类共有10万条基因,基因中 DNA 的排列次序就是蛋白质合成的遗传密码,即生物的基因指令,不仅决定了我们头发与眼睛的颜色、身高、相貌,也决定了
基金This work was supported by the National Basic Research Program of China (2009CB941000).
文摘Although the somatic cell nuclear transfer(SCNT) technique has been used extensively for cloning and generating transgenic pigs,the cloning efficiency is still very low.It has been proposed that the low efficiency of this technique is the result of incomplete epigenetic reprogramming and abnormal gene expression during early embryonic development.In this study,we investigate the effect of Scriptaid,a low-toxicity histone deacetylase inhibitor,on the developmental competence of porcine SCNT embryos.We found that treating SCNT embryos with 500 nmol/L Scriptaid for 15 h after activation significantly enhanced the blastocyst formation rate(27.7%) compared with the untreated group(control)(12.2%,P<0.05).Using an immunofluorescence technique to measure the average fluorescence intensity,we also found that treating SCNT embryos with Scriptaid increased the level of histone acetylation on histone H3 at lysine 14(acH3K14).Furthermore,treating embryos with Scriptaid increased the expression level of three genes that play important roles during embryonic development(Oct4,Klf4 at the blastocyst stage and Nanog at the 4-cell stage).Moreover,the expression level of the apoptosis-related gene Caspase-3 was significantly lower in the Scriptaid-treated SCNT embryos compared with the control SCNT embryos at the 4-cell and blastocyst stages.In conclusion,these results indicate that Scriptaid treatment improves the development and nuclear reprogramming of porcine SCNT embryos.