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加味茵陈蒿汤联合熊去氧胆酸治疗原发性胆汁性胆管炎的临床疗效及对FGF19的影响 认领
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作者 凌琪华 郑月琪 +6 位作者 陈建杰 乐敏 时桢 徐黎 陈逸云 卓蕴慧 商斌仪 《上海中医药杂志》 2020年第S01期41-43,共3页
目的评价加味茵陈蒿汤联合熊去氧胆酸治疗原发性胆汁性胆管炎的临床疗效及对血清FGF19的影响。方法将64例肝胆湿热兼瘀血阻络型原发性胆汁性胆管炎患者随机分为治疗组和对照组,每组32例。治疗组给予加味茵陈蒿汤联合熊去氧胆酸治疗,对... 目的评价加味茵陈蒿汤联合熊去氧胆酸治疗原发性胆汁性胆管炎的临床疗效及对血清FGF19的影响。方法将64例肝胆湿热兼瘀血阻络型原发性胆汁性胆管炎患者随机分为治疗组和对照组,每组32例。治疗组给予加味茵陈蒿汤联合熊去氧胆酸治疗,对照组给予熊去氧胆酸治疗。两组疗程均为12个月,观察中医证候积分、生化学应答率、肝脏生化指标、血清FGF19的变化情况。结果①治疗后,治疗组、对照组的生化学应答率分别为87.5%、62.5%,组间差异有统计学意义(P<0.05);②治疗后,治疗组血清ALT、AST、GGT、ALP水平均较对照组下降,差异有统计学意义(P<0.05);③治疗后,治疗组中医证候积分低于对照组,差异有统计学意义(P<0.05);④治疗后,治疗组血清FGF19水平较对照组升高,差异有统计学意义(P<0.05)。结论加味茵陈蒿汤联合熊去氧胆酸治疗肝胆湿热兼瘀血阻络型原发性胆汁性胆管炎,能够有效提高生化学应答率,可改善血清ALT、AST、GGT、ALP水平及中医证候积分,提高血清FGF19水平。 展开更多
关键词 原发性胆汁性胆管炎 中西医结合疗法 加味茵陈蒿汤 熊去氧胆酸 成纤维细胞生长因子19
人工肝影响乙肝慢加急性肝衰竭患者细胞因子表达的意义 认领
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作者 郑桂萍综述 张斌审校 《文摘版:医药卫生》 2015年第8期20-20,23共2页
乙肝慢加急性肝衰竭(HBV-ACLF)的发病机制复杂,细胞因子级联释放所介导的免疫损伤是造成肝细胞大量坏死的重要机制之一,在 ACLF 的 病 程 中 细 胞 因 子 起 到 重 要 作 用,大量研究表明,ACLF 患者血清细胞因子水平明显增高,故... 乙肝慢加急性肝衰竭(HBV-ACLF)的发病机制复杂,细胞因子级联释放所介导的免疫损伤是造成肝细胞大量坏死的重要机制之一,在 ACLF 的 病 程 中 细 胞 因 子 起 到 重 要 作 用,大量研究表明,ACLF 患者血清细胞因子水平明显增高,故针对这一发病机制,有效的清除炎性细胞因子,从而减轻肝细胞的免疫损害,对缓解肝衰竭患者病情、改善其预后有着重要意义。近年来人工肝治疗已成为治疗肝衰竭的重要手段,血浆置换等人工肝治疗方式已被证实对各 种炎性细胞因子有清除作用,可阻断或减轻细胞因子介导的各种病理生理过程,从而改善患者临床症状及预后,在乙肝慢加急性肝衰竭治疗中有着重要意义,并有待进一步探讨。 展开更多
关键词 乙肝慢加急性肝衰竭 人工肝 细胞因子.
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胆囊检查需注意事项 认领
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作者 罗颖 《医药界》 2019年第15期0188-0188,共1页
胆囊位于肝脏右叶脏面的胆囊窝内。容量 40-60ml,胆囊分为底、体、颈部。有储存、 浓缩胆汁的功能。胆囊正常超声表现,纵切类似梨形、长茄形,横切接近圆形或椭圆形,颈 部可有分隔稍弯曲指向肝门方向,长径约 9cm,前后径一般小于 3.5cm,... 胆囊位于肝脏右叶脏面的胆囊窝内。容量 40-60ml,胆囊分为底、体、颈部。有储存、 浓缩胆汁的功能。胆囊正常超声表现,纵切类似梨形、长茄形,横切接近圆形或椭圆形,颈 部可有分隔稍弯曲指向肝门方向,长径约 9cm,前后径一般小于 3.5cm,胆囊壁回声清晰, 内壁光滑,厚约 0.1-0.3cm,腔内为清晰无回声液区,后壁及后方回声可增强。 展开更多
关键词 胆囊 注意事项
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梗阻性黄疸224例分析 认领 被引量:6
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作者 李柯 姚炜 +1 位作者 常虹 黄永辉 《中国现代医学杂志》 CAS CSCD 北大核心 2008年第22期共4页
目的提高对梗阻性黄疸的认识和诊疗水平。方法对2004~2007年北京大学第三医院收治的224例梗阻性黄疸患者病因、临床表现、生化特征、影像学资料及治疗进行总结分析。结果胰胆系统恶性肿瘤和胆管结石仍是导致梗阻性黄疸的主要原因,原发... 目的提高对梗阻性黄疸的认识和诊疗水平。方法对2004~2007年北京大学第三医院收治的224例梗阻性黄疸患者病因、临床表现、生化特征、影像学资料及治疗进行总结分析。结果胰胆系统恶性肿瘤和胆管结石仍是导致梗阻性黄疸的主要原因,原发性胆管癌明显增加。黄疸重、体重减轻,而腹痛和发热相对不明显是恶性梗阻性黄疸的特点。肿瘤标记物、特别是CA19-9的检测在良恶性疾病的鉴别上起重要作用,但胆道疾病合并胆汁郁积时CA19-9的特异性低(39.1%)。MRCP和ERCP检查准确性高(分别为92.9%和94.1%),两者相比差异无显著性(P>0.05),但ERCP常同时发挥诊断和治疗的双重作用。手术和介入是梗阻性黄疸的主要治疗手段。结论应进一步提高对恶性疾病导致梗阻性黄疸重要性的认识,综合分析患者的临床表现、生化特征及影像学,治疗应采用以病因治疗为主的综合治疗措施,充分发挥微创技术的优势。 展开更多
关键词 梗阻性黄疸 诊断 治疗
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在 cholangiocyte 功能的调整的性荷尔蒙的角色 认领
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作者 Romina Mancinelli Paolo Onori +6 位作者 Sharon DeMorrow Heather Francis Shannon Glaser Antonio Franchitto Guido Carpino Gianfranco Alpini Eugenio Gaudio 《世界胃肠病理生理学杂志:英文版(电子版)》 2010年第2期50-62,共13页
Over the last years,cholangiocytes,the cells that line the biliary tree,have been considered an important object of study for their biological properties which involves bile formation,proliferation,injury repair,fibr... Over the last years,cholangiocytes,the cells that line the biliary tree,have been considered an important object of study for their biological properties which involves bile formation,proliferation,injury repair,fibrosis and angiogenesis.Cholangiocyte proliferation occurs in all pathologic conditions of liver injury where it is associated with inflammation and regeneration.During these processes,biliary cells start to secrete different cytokines,growth factors,neuropeptides and hormones which represent potential mechanisms for cross talk with other liver cells.Several studies suggest that hormones,and in particular,sex hormones,play a fundamental role in the modulation of the growth of this compartment in the injured liver which functionally conditions the progression of liver disease.Understanding the mechanisms of action and the intracellular pathways of these compounds on cholangiocyte pathophysiology will provide new potential strategies for the management of chronic liver diseases.The purpose of this review is to summarize the recent findings on the role of sex hormones in cholangiocyte proliferation and biology. 展开更多
关键词 BILIARY EPITHELIUM SEX HORMONES Cholestatic DISEASES
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Genetic polymorphism and mRNA levels of cytochrome P450ⅡE1 and glutathione S-transferase P1 in patients with alcoholic liver disease in different nationalities 认领
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《国际肝胆胰疾病杂志:英文版》 SCIE CAS 2009年第2期162-167,共6页
BACKGROUND:Alcohol abuse and dependence are major factors in the pathogenesis of alcoholic liver disease(ALD).Alcohol abuse is becoming an increasingly severe problem among the Han,Mongol,and Korean nationalities in ... BACKGROUND:Alcohol abuse and dependence are major factors in the pathogenesis of alcoholic liver disease(ALD).Alcohol abuse is becoming an increasingly severe problem among the Han,Mongol,and Korean nationalities in northeast China.This study aimed to investigate the relationship between ALD and the genetic polymorphism and expression levels of two enzymes,cytochrome P450ⅡE1(CYPⅡE1)and glutathione S-transferase P1(GSTP1)in patients of three nationalities.METHODS:Peripheral blood was collected from 353 Chinese patients with ALD,300 alcohol dependent patients without liver disease(alcoholic),and 360 healthy controls.Each group included patients from the Han,Mongol and Korean nationalities.Real-time polymerase chain reaction(PCR)and PCR-restriction fragment length polymorphism(PCR-RFLP)were used.RESULTS:Regardless of nationality,patients who carried the rare CYPⅡE1 C2 and GSTP1 Val alleles were at higher risk of ALD.The frequency of C2 and Val in patients with ALD was respectively 50.00% and 26.98% in the Han,31.36% and 22.87% in the Mongol,and 45.87% and 22.02% in the Korean nationality.No significant differences were seen in the frequency of either C2 or Val alleles in ALD patients among the three nationalities.In each nationality,the frequency of both C2 and Val alleles was significantly higher in ALD compared to alcoholic and healthy controls.Except for nationality,the average mRNA levels of CYPⅡ E1 in ALD patients and healthy controls were 10.05%and 2.21%,respectively.The average mRNA levels of GSTP1 in ALD patients and healthy controls were 0.53%and 2.12%,respectively.The mRNA level of CYPⅡE1 was higher,and that of GSTP1 was lower in patients with ALD compared to the controls.CONCLUSIONS:Except for nationality,patients with ALD in this series tended to have a higher mRNA expression of CYPⅡE1 and to carry the C2 allele,and tended to have a lower mRNA expression of GSTP1 and to carry the Val allele.There is a causal relationship between the polymorphic alleles,which leads to different mRNA levels an 展开更多
关键词 ALCOHOLIC liver disease ALCOHOLIC GENETIC POLYMORPHISM mRNA expression
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Preconditioning and postconditioning reduce hepatic ischemia-reperfusion injury in rats 认领 被引量:1
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作者 Zhang, Wan-Xing Yin, Wen +5 位作者 Zhang, Lei Wang, Lan-Hui Bao, Lei Tuo, Hong-Fang Zhou, Li-Fang Wang, Chun-Cheng 《国际肝胆胰疾病杂志:英文版》 SCIE CAS 2009年第6期586-590,共5页
BACKGROUND:Ischemia-reperfusion injury occurs when ischemic tissues or organs suffer from further functional and structural damage when their blood supply recovers.This study aimed to contrast the protective effects ... BACKGROUND:Ischemia-reperfusion injury occurs when ischemic tissues or organs suffer from further functional and structural damage when their blood supply recovers.This study aimed to contrast the protective effects of ischemic preconditioning and ischemic postconditioning in hepatic ischemia-reperfusion injury in rats.METHODS:Thirty-two healthy male Wistar rats were randomly divided into four groups:sham-operated(SO),ischemia-reperfusion(IR),ischemic preconditioning(I-pre),and ischemic postconditioning(I-post).Blood samples and hepatic tissue were taken from all groups after the experiments.RESULTS:There were significant differences between the IR,I-pre and I-post groups in alanine aminotransferase and aspartate aminotransferase levels,NF-κB p65 expression,apoptosis index and superoxide dismutase activity in hepatic tissue.There were no significant differences between the I-pre and I-post groups.CONCLUSIONS:Ischemic postconditioning and ischemic preconditioning reduce hepatic ischemia-reperfusion injury,but in clinical practice the former is a more appropriate choice. 展开更多
关键词 liver ISCHEMIA-REPERFUSION INJURY ISCHEMIC POSTCONDITIONING ISCHEMIC PRECONDITIONING NF-κB
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Desensitization of G-protein-coupled receptors induces vascular hypocontractility in response to norepinephrine in the mesenteric arteries of cirrhotic patients and rats 认领
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作者 Wei Chen Jiang-Yong Sang +4 位作者 De-Jun Liu Jun Qin Yan-Miao Huo Jia Xu Zhi-Yong Wu 《国际肝胆胰疾病杂志:英文版》 SCIE CAS 2013年第3期295-304,共10页
BACKGROUND: The increased β-arrestin-2 and its combination with G-protein-coupled receptors (GPCRs) lead to GPCRs desensitization. The latter may be responsible for decreased contractile reactivity in the mesenteric... BACKGROUND: The increased β-arrestin-2 and its combination with G-protein-coupled receptors (GPCRs) lead to GPCRs desensitization. The latter may be responsible for decreased contractile reactivity in the mesenteric arteries of cirrhotic patients and rats. The present study is to investigate the machinery changes of α-adrenergic receptors and G proteins and their roles in the contractility of mesenteric arteries of cirrhotic patients and animal models. METHODS: Patients with cirrhosis due to hepatitis B and cirrhotic rats induced by CCl 4 were studied. Mesenteric artery contractility in response to norepinephrine was determined by a vessel perfusion system. The contractile effect of G protein-coupled receptor kinase-2 (GRK-2) inhibitor on the mesenteric artery was evaluated. The protein expression of the α 1 adrenergic receptor, G proteins, β-arrestin-2, GRK-2 as well as the activity of Rho associated coiled-coil forming protein kinase-1 (ROCK-1) were measured by Western blot. In addition, the interaction of α 1 adrenergic receptor with β-arrestin-2 was assessed by co-immunoprecipitation. RESULTS: The portal vein pressure of cirrhotic patients and rats was significantly higher than that of controls. The doseresponse curve to norepinephrine in mesenteric arteriole was shifted to the right, and EC 50 was significantly increased in cirrhotic patients and rats. There were no significant differences in the expressions of the α 1 adrenergic receptor and G proteins in the cirrhotic group compared with the controls. However, the protein expressions of GRK-2 and β-arrestin-2 were significantly elevated in cirrhotic patients and rats compared with those of the controls. The interaction of the α 1 adrenergic receptor and β-arrestin-2 was significantly aggravated. This interaction was significantly reversed by GRK-2 inhibitor. Both the protein expression and activity of ROCK-1 were significantly decreased in the mesenteric artery in patients with cirrhosis compared with those of the controls, and this phenomenon w 展开更多
关键词 portal hypertension DESENSITIZATION G-protein-coupled receptors β-arrestin-2 RHO associated COILED-COIL forming protein kinase
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黄疸不等于肝炎 认领
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作者 赵国根 《健康博览》 2002年第12期17-17,共1页
黄疸是肝炎的主要表现,但出现黄疸的并一定都是患了肝炎。以治疗肝病为主的杭州市第六人民医院的医生都碰到过因为黄疸而以为是患了肝炎前来诊治的病人,虽然有经验的专家通过检查能做出明确的诊断,但往往这些病人都已有四处求医,长时... 黄疸是肝炎的主要表现,但出现黄疸的并一定都是患了肝炎。以治疗肝病为主的杭州市第六人民医院的医生都碰到过因为黄疸而以为是患了肝炎前来诊治的病人,虽然有经验的专家通过检查能做出明确的诊断,但往往这些病人都已有四处求医,长时间被当作肝炎而进行不合理治疗的经历。不久前,一位年才30的患者,10多年前就开始出现黄疸,一直被当作肝炎,多处求医、吃药,不见好转,并因病找不到对象。偶然,通过一个健康咨询热线介绍,找到医院的一位专家。经过 展开更多
关键词 肝炎 黄疸 主要表现 合理治疗 咨询热线 长时间 病人 杭州市 肝病患者 诊断治疗
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解开脂肪肝的疑团 认领
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作者 王虎生 《祝您健康》 1998年第7期37-37,共1页
随着人们生活水平的提高,脂肪肝的发生率也渐渐高起来了。门诊医生几乎每天都能碰到这样的问题:脂肪肝是怎么回事?有什么特效药可以治疗?有些人本来感觉良好,经 B 超检查后戴上了脂肪肝的帽子,就如同坠入云里雾里,疑团顿生。他们担心... 随着人们生活水平的提高,脂肪肝的发生率也渐渐高起来了。门诊医生几乎每天都能碰到这样的问题:脂肪肝是怎么回事?有什么特效药可以治疗?有些人本来感觉良好,经 B 超检查后戴上了脂肪肝的帽子,就如同坠入云里雾里,疑团顿生。他们担心脂肪肝的危害,整天感到上腹 展开更多
关键词 门诊医生 多烯康 地奥脂必妥 上腹 降脂药物 舒降之 生活水平 脂血症 酒精性肝炎 慢性酒精中毒
治疗重型肝炎7种方法的效果评价 认领 被引量:1
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作者 何艳 韦怡 +1 位作者 李(钅从) 苏先狮 《实用肝脏病杂志》 CAS 1997年第4期244-245,共2页
为了探讨重型肝炎不同治疗方法的效果,本文分析本院1993~1996年203例重型肝炎应用7种方法治疗的效果,报告如下。
关键词 重型肝炎 血浆置换 法的效果 综合治疗 有效率 脐血 并发症 肿瘤坏死因子 治疗组 湖南医科大学
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中西医结合肝病论文主题标引述要 认领
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作者 姚昌绶 《中西医结合肝病杂志》 CAS 1991年第1期55-57,共3页
一、何谓标引?标引工作有哪些常用术语? 所谓标引,就是按照一定的方法和规则,对图书资料中具有检索意义的一些内部特征(包括分类、主题、提要等)和外表特征(包括书名或篇名、著者、出版年、出版地、文种等)进行分析、著录,给出主题词... 一、何谓标引?标引工作有哪些常用术语? 所谓标引,就是按照一定的方法和规则,对图书资料中具有检索意义的一些内部特征(包括分类、主题、提要等)和外表特征(包括书名或篇名、著者、出版年、出版地、文种等)进行分析、著录,给出主题词、分类号等检索标志。它有码号与词语两大系统,对文献给予分类号的过程,称为 展开更多
关键词 主题标引 检索标志 中西医结合 分类号 标引人员 码号 外表特征 图书资料 副主题词 主题词表
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The expression of HIF-1 α in liver tissues in the rat model of paraquat poisoning 认领
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作者 熊莺 《中国医学文摘:内科学分册(英文版)》 2014年第3期179-180,共2页
Objective To observe the levels of HIF-αand TGF-βin the liver tissue,change of serum transaminase in different phases after paraquat(PQ)toxicity and liver histopathology change in PQ-induced liver toxicity of rat m... Objective To observe the levels of HIF-αand TGF-βin the liver tissue,change of serum transaminase in different phases after paraquat(PQ)toxicity and liver histopathology change in PQ-induced liver toxicity of rat models in order to analyze the relationship between HIF-αand hepatic toxicity induced by PQ.Methods A total of 48 healthy SD rats were randomly(random number)assigned into 2 groups:PQ poisoning group(n=42, 展开更多
关键词 PARAQUAT POISONING toxicity assigned INFILTRATION exposure cytes ALANINE hepato SALINE
急性肝坏死小鼠血脑屏障通透性的改变 认领 被引量:8
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作者 吕飒 宋红丽 +1 位作者 王静艳 刘沛 《世界华人消化杂志》 CAS 2004年第6期1346-1348,共3页
目的:探讨急性肝坏死动物血脑屏障通透性异常改变方法:将280只♂Balb/c小鼠分为4N,应用内毒素(LPS,10μg/kg)和D-氨基半乳糖(GAIN,800mg/kg)联合ip,建立急性肝坏死小鼠模型.并检测血清ALT,观察肝脏组织病理学变化,利用伊文... 目的:探讨急性肝坏死动物血脑屏障通透性异常改变方法:将280只♂Balb/c小鼠分为4N,应用内毒素(LPS,10μg/kg)和D-氨基半乳糖(GAIN,800mg/kg)联合ip,建立急性肝坏死小鼠模型.并检测血清ALT,观察肝脏组织病理学变化,利用伊文思蓝研究小鼠血脑屏障通透性的改变情况.结果:单独应用LPS或GaIN仅使血清ALT轻度升高,动物无死亡.而联合应用后ALT则从6h开始明显升高(41.89±14.57μkat/L),到12h达高峰(170.30±16.13μkat/L),较其他各组均有显著性差异(P<0.01).动物从6h开始死亡,9h达高峰,总死亡率达66.6%.肝脏HE染色可见大块或亚大块出血性坏死,而其他组仅见单个或灶状肝细胞坏死,部分细胞脂肪变性.并且脑组织EB含量在各时间点均较其他组明显升高.结论:在急性肝坏死动物中存在血脑屏障通透性异常增加的改变,可能是引起脑水肿发生的重要机制. 展开更多
关键词 急性肝坏死 小鼠 血脑屏障 通透性 脑水肿
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苦参碱联合复方鳖甲软肝片抗肝纤维化的疗效观察 认领 被引量:3
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作者 蒋忠胜 《肝脏》 2004年第2期 143,共1页
我们采用苦参碱联合复方鳖甲软肝片治疗慢性乙型病毒性肝炎,观察其抗肝纤维化疗效.
关键词 苦参碱 联合 复方鳖甲软肝片 抗肝纤维化 疗效观察
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急性坏疽性胆囊炎的临床特点和外科诊治体会 认领
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作者 沈铁骁 《蚌埠医学院学报》 CAS 2004年第5期 456-457,共2页
目的:探讨急性坏疽性胆囊炎的临床特点和外科诊治方法.方法:对1993~2002年外科收治的118例急性坏疽性胆囊炎患者临床特点和外科诊治资料进行回顾性分析.结果:急性坏疽性胆囊炎多由急性胆囊炎发展而来,多发生于老年患者,进展迅速,病情凶... 目的:探讨急性坏疽性胆囊炎的临床特点和外科诊治方法.方法:对1993~2002年外科收治的118例急性坏疽性胆囊炎患者临床特点和外科诊治资料进行回顾性分析.结果:急性坏疽性胆囊炎多由急性胆囊炎发展而来,多发生于老年患者,进展迅速,病情凶险,明确诊断后立即手术治疗,本组118例均行手术治疗,治愈114例,病死4例.结论:早期诊断,完善的术前准备,积极的手术治疗是提高治愈率的关键. 展开更多
关键词 胆囊炎 坏疽 外科手术
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脂肪肝饮食建议 认领
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《现代中医药(北京)》 2004年第16期 58-59,共2页
本人任职公司主管,工作上有很多应酬,整天酒肉不断。最近在身体检查中发现自己患上了脂肪肝,血清丙氨酸氨基转移酶出现异常。虽然没有自觉症状,但朋友说脂肪肝会引起肝硬化,预後极差,而且再也不能吃脂肪类食物了。这种说法正确吗... 本人任职公司主管,工作上有很多应酬,整天酒肉不断。最近在身体检查中发现自己患上了脂肪肝,血清丙氨酸氨基转移酶出现异常。虽然没有自觉症状,但朋友说脂肪肝会引起肝硬化,预後极差,而且再也不能吃脂肪类食物了。这种说法正确吗:脂肪肝应该如何治疗:日常生活中应该怎样调养: 展开更多
关键词 脂肪肝 饮食 治疗 自觉症状 血清丙氨酸氨基转移酶 调养 肝硬化 任职 主管 公司
Effects of Zuogui Wan on neurocyte apoptosis and down-regulation of TGF-β1 expression in nuclei of arcuate hypothalamus of monosodium glutamate -liver regeneration rats 认领 被引量:6
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作者 Han-MinLi XiangGao +3 位作者 Mu-LanYang, Jia-JunMei Liu-TongZhang Xing-FanQiu 《世界胃肠病学杂志:英文版(电子版)》 SCIE CAS CSCD 2004年第19期2823-2826,共4页
AIM: To inquire into the effects and mechanism of Zuogui Wan (Pills for Kidney Yin) on neurocyte apoptosis in nuclei of arcuate hypothalamus (ARN) of monosodium glutamate (MSG)-Iiver regeneration rats, and the mechani... AIM: To inquire into the effects and mechanism of Zuogui Wan (Pills for Kidney Yin) on neurocyte apoptosis in nuclei of arcuate hypothalamus (ARN) of monosodium glutamate (MSG)-Iiver regeneration rats, and the mechanism of liver regeneration by using optic microscope, electron microscope and in situ end labeling technology to adjust nerve-endocrine-immunity network.METHODS: Neurocyte apoptosis in ARN of the experiment rats was observed by using optic microscope, electron microscope and in situ end labeling technology. Expression of TGF-β1 in ARN was observed by using immunohistochemistry method.RESULTS: The expression of TGF-β1 in rats of model group was increased with the increase of ARN neurocyte apoptosis index (AI) (t = 8.3097, 12.9884, P<0.01). As compared with the rats of model group, the expression of TGF-β1 in rats of Zuogui Wan treatment group was decreased with the significant decrease of ARN neurocyte apoptosis (t = 4.5624, 11.1420, P<0.01).CONCLUSION: Brain neurocyte calcium ion overexertion and TGF-β1 protein participate in the adjustment and control of ARN neurocyte apoptosis in MSG-liver regeneration-rats. Zuogui Wan can prevent ARN neurocyte apoptosis of MSG-liver regeneration in rats by downregulating the expression of TGF-β1, and influence liver regeneration through adjusting nerve-endocrine-immunen etwork. 展开更多
关键词 神经细胞 细胞凋亡 向下准则 TGF-Β1 基因表达 胞核 弓形虫 视丘下部 谷氨酸单钠盐 肝再生 老鼠
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以左上肢无力为首发症状的肝性脑病一例 认领
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作者 曲兵兵 《山西医药杂志》 CAS 2005年第2期 96,共1页
患者女,66岁.因左上肢活动不能2 d,加重伴头晕半天入院.2 d前无诱因出现左上肢无力,抬起困难,未在意.半天前诉头晕,家属发现其左上肢活动不能、反应淡漠、不吃不喝、言语含糊、答非所问遂来诊.既往"肺源性心脏病"病史10余年.... 患者女,66岁.因左上肢活动不能2 d,加重伴头晕半天入院.2 d前无诱因出现左上肢无力,抬起困难,未在意.半天前诉头晕,家属发现其左上肢活动不能、反应淡漠、不吃不喝、言语含糊、答非所问遂来诊.既往"肺源性心脏病"病史10余年.查体:血压130/90 mm Hg(1 mm Hg=0.133 kPa),面色萎黄,神志恍惚,巩膜中度黄染,未见蜘蛛痣及肝掌;腹部膨隆,肝脾触诊不清,移动性浊音(+),肠鸣音活跃.双下肢凹陷性水肿;左上肢肌力Ⅰ级,肌张力低,余肢体肌力、肌张力正常,病理征(-). 展开更多
关键词 上肢活动 头晕 肝性脑病 首发症状 神志 查体 巩膜 困难 家属 患者
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Effects of a plasmid expressing antisense tissue inhibitor of metalloproteinase-1 on liver fibrosis in rats 认领 被引量:4
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作者 JIANGWei WANGJi-yao YANGChang-qing LIUWen-bin WANGYi-qing HEBo-ming 《中华医学杂志:英文版》 SCIE CAS CSCD 2005年第3期 192-197,共6页
Background No efficient therapy for liver fibrosis has been available. This study was aimed to provide evidence that the introduction of a plasmid expressing antisense tissue inhibitor of metalloproteinase-1 (TIMP-1) ... Background No efficient therapy for liver fibrosis has been available. This study was aimed to provide evidence that the introduction of a plasmid expressing antisense tissue inhibitor of metalloproteinase-1 (TIMP-1) into a rat model of immunologically induced liver fibrosis can result in the increased activity of interstitial collagenase, thus enhancing the degradation of collagen.Methods Real-time nested polymerase chain reaction (RT-Nested-PCR) and gene recombination techniques were used to construct a rat antisense TIMP-1 recombinant plasmid that can be expressed in eukaryotic cells. Both the recombinant plasmid and an empty vector (pcDNA3) were encapsulated with glycosyl-poly-L-lysine and injected into rats suffering from pig serum-induced liver fibrosis. The expression of exogenous transfected plasmid was assessed by Northern blot, RT-PCR, and Western blot. Hepatic interstitial collagenase activity was detected using fluorescinisothiocyanate (FITC)-labeled type Ⅰ collagen. In addition to hepatic hydroxyproline content, hepatic collagen types Ⅰ and Ⅲ were detected by immunohistochemical staining, and the stages of liver fibrosis by Van Gieson staining.Results Exogenous antisense TIMP-1 was successfully expressed in vivo and could block the gene and protein expression of TIMP-1. Active and latent hepatic interstitial collagenase activities were elevated (P<0.01), hepatic hydroxyproline content and the accumulation of collagen types Ⅰ and Ⅲ were lowered, and liver fibrosis was alleviated in the antisense TIMP-1 group (P<0.01) as compared with the model group. Conclusion The results demonstrate that antisense TIMP-1 recombinant plasmids have some inhibitory effect on liver fibrosis. 展开更多
关键词 质粒 基因表达 抗过敏作用 组织抑制剂 金属蛋白-1 老鼠 肝纤维化 消化系统
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