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新鲜烟叶中的1个新二萜类成分 被引量:1
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作者 郑庆霞 刘萍萍 +4 位作者 翟妞 陈千思 金立锋 陈霞 周会娜 《中草药》 CSCD 北大核心 2017年第13期2597-2600,共4页
目的研究烟草Nicotiana tabacum叶中的西松烷二萜类化学成分。方法采用柱色谱技术对烟草的95%乙醇提取物进行分离纯化,采用波谱技术结合化学方法进行结构鉴定。结果从烟草提取物中分离得到了5个西松烷二萜类成分,分别鉴定为(1R,3E,7E,1... 目的研究烟草Nicotiana tabacum叶中的西松烷二萜类化学成分。方法采用柱色谱技术对烟草的95%乙醇提取物进行分离纯化,采用波谱技术结合化学方法进行结构鉴定。结果从烟草提取物中分离得到了5个西松烷二萜类成分,分别鉴定为(1R,3E,7E,10S,11E)-3,7,11-cem-bratriene-10,15-diols(1)、(1S,2E,4S,6R,7E,10E,12S)-2,7,10-cembratriene-4,6,11-triol(2)、(1S,2E,4R,6R,7E,10E,12S)-2,7,10-cembratriene-4,6,11-triol(3)、(1S,2E,4S,6R,7E)-4,6,11-trihydroxy-1-isopropyl-4,8-dimethylpentadeca-2,7-dien-12-one(4)、(1S,2E,4R,6R,7E,11S,12S)-11,12-epoxy-2,7-cembradiene-4,6-diol(5)。结论化合物1为1个新化合物,命名为烟叶二萜B;化合物4首次在植物中发现,为1个新的天然产物。 展开更多
关键词 烟草属 烟叶 西松烷二萜 烟叶二萜B (1S 2E 4S 6R 7E)-4 6 11-trihydroxy-1-isopropyl-4 8-dimethylpentadeca-2 7-dien-12-one
人空变载脂蛋白E(apoE4,apoe7)转基因小鼠模型 被引量:3
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作者 孙明增 琦祖和 《中国科学:C辑》 CSCD 北大核心 2001年第1期1-6,共6页
为揭示人突变apoE基因表达在整体引起的异常反应并得到人类相关疾病的小鼠模型,用显微注射法制备了人apoE4与apoE7转基因小鼠,用Southern印迹杂交及ELISA证明人apoE基因在首建鼠及其子代体内整合与表达良好,具有遗传稳定性,血清脂... 为揭示人突变apoE基因表达在整体引起的异常反应并得到人类相关疾病的小鼠模型,用显微注射法制备了人apoE4与apoE7转基因小鼠,用Southern印迹杂交及ELISA证明人apoE基因在首建鼠及其子代体内整合与表达良好,具有遗传稳定性,血清脂质与行为测定表明,人apoE表达使小鼠患高脂血症并出现大脑学习与记忆功能衰退和寿命缩短,高脂食物对正常鼠均可诱发高血脂,但机制不同。同时还证明,氨基端与羧基端突变的apoE具有相同的致病作用。 展开更多
关键词 转基因鼠 人APOE7/E4 高脂血症 学习 记忆 寿命 载脂蛋白E
The human apoE7 and apoE4 transgenic mice models
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作者 SUN Mingzeng ( 《中国科学:生命科学英文版》 SCIE CAS 2001年第6期652-660,共9页
To scrutinize the disorders caused by human mutant apoE7/apoE4, human apoE4 and E7 transgenic mice were established with microinjection technique to examine molecular genetic phenomena in vivo. The integration and exp... To scrutinize the disorders caused by human mutant apoE7/apoE4, human apoE4 and E7 transgenic mice were established with microinjection technique to examine molecular genetic phenomena in vivo. The integration and expression of h-apoE mutant genes in transgenic mice were determined with Southern blot, Northern blot and ELISA. The current studies indicated that the transgenes and the phenotypes regarding expression of transgenes could be transmitted stably in transgenic lines. The levels of serum lipid in transgenic mice showed the characteristics of hyperlipidemia. Besides, behavior tests demonstrated the degeneration of learning and memory in transgenic mice. Short life span was observed in 2 transgenic lines. After fed with high lipid food high serum lipid was found both in normal and transgenic mice, but their mechanism regulating lipid metabolism was different. It was also verified that the human apoE mutants located at either N-terminal or C-terminal had the same pathogenesis regarding disorders of lipid metabolism in murine. 展开更多
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