期刊文献+
共找到4,398篇文章
< 1 2 220 >
每页显示 20 50 100
Engineering the Biosynthesis of Caffeic Acid in Saccharomyces cerevisiae with Heterologous Enzyme Combinations 预览
1
作者 Lanqing Liu Hong Liu +4 位作者 Wei Zhang Mingdong Yao Bingzhi Li Duo Liu Yingjin Yuan 《工程(英文)》 2019年第2期287-295,共9页
Engineering the biosynthesis of plant-derived natural products in microbes presents several challenges, especially when the expression and activation of the plant cytochrome P450 enzyme is required. By recruiting two ... Engineering the biosynthesis of plant-derived natural products in microbes presents several challenges, especially when the expression and activation of the plant cytochrome P450 enzyme is required. By recruiting two enzymes—HpaB and HpaC—from several bacteria, we constructed functional 4- hydroxyphenylacetate 3-hydroxylase (4HPA3H) in Saccharomyces cerevisiae to take on a role similar to that of the plant-derived cytochrome P450 enzyme and produce caffeic acid. Along with a common tyrosine ammonia lyase (TAL), the different combinations of HpaB and HpaC presented varied capabilities in producing the target product, caffeic acid, from the substrate, L-tyrosine. The highest production of caffeic acid was obtained with the enzyme combination of HpaB from Pseudomonas aeruginosa and HpaC from Salmonella enterica, which yielded up to (289.4 ± 4.6) mgL1 in shake-flask cultivation. The compatibility of heterologous enzymes within a yeast chassis was effectively improved, as the caffeic acid production was increased by 40 times from the initial yield. Six key amino acid residues around the flavin adenine dinucleotide (FAD) binding domain in HpaB from Pseudomonas aeruginosa were differentiate from those other HpaBs, and might play critical roles in affecting enzyme activity. We have thus established an effective approach to construct a highly efficient yeast system to synthesize non-native hydroxylated phenylpropanoids. 展开更多
关键词 SACCHAROMYCES CEREVISIAE Caffeic acid HETEROLOGOUS enzyme CYTOCHROME P450 Synthetic biology
在线阅读 免费下载
藻类鉴定及分类方法研究进展 预览
2
作者 孟溪 《生物化工》 2019年第2期102-104,共3页
藻类是地球上的初级生产者,通过光合作用平衡大气成分、保护环境生态。藻类的多样性也是自然中重要的生物资源,准确快捷的鉴定方法对于研究和分析藻类具有十分重要的作用。随着分析仪器的革新以及研究的深入,鉴定分类方法也由传统的依... 藻类是地球上的初级生产者,通过光合作用平衡大气成分、保护环境生态。藻类的多样性也是自然中重要的生物资源,准确快捷的鉴定方法对于研究和分析藻类具有十分重要的作用。随着分析仪器的革新以及研究的深入,鉴定分类方法也由传统的依靠形态、细胞色素逐渐向DNA分子鉴定发展。随着时代发展及科技进步,藻类资源必将更好地被研究分析并加以利用。 展开更多
关键词 藻类 形态鉴定 细胞色素
在线阅读 下载PDF
A membrane separation technique for optimizing sample preparation of MALDI-TOF MS detection
3
作者 Zengnan Wu Ning Xu +1 位作者 Weiwei Li Jin-Ming Lin 《中国化学快报:英文版》 SCIE CAS CSCD 2019年第1期95-98,共4页
The performance of matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) is frequently compromised by the high limited of detection, heterogeneous distribution of matrix and analyt... The performance of matrix-assisted laser desorption/ionization time of flight mass spectrometry(MALDI-TOF-MS) is frequently compromised by the high limited of detection, heterogeneous distribution of matrix and analyte deposits and time-consuming drying time during the conventional drop drying procedure. Here, we describe a method based on membrane separation technology to assist in the drying of nanomaterials to rapidly make homogeneous sample spots and to enhance the mass signal. We demonstrate that the sample can be dried evenly in a few seconds and MS signal can be greatly optimized by using membrane separation technology. Enrichment with nanomaterials greatly enhances analyte signal strength while membrane separation method avoids the time-consuming requirements of searching for “sweet spots”. 展开更多
关键词 MALDI-MS Membrane separation NANOMATERIAL MASTOPARAN INSULIN CYTOCHROME C
Association between CYP2C19*2/*3Polymorphisms and Coronary Heart Disease 预览
4
作者 Ying-ying ZHANG Xin ZHOU +4 位作者 Wen-jie JI Ting LIU Jing MA Ying ZHANG Yu-ming LI 《当代医学科学(英文)》 SCIE CAS 2019年第1期44-51,共8页
This study sought to explore the relationship between cytochrome P450 2C19(CYP2C19)*2/*3 polymorphisms and the development of coronary heart disease(CHD),and to evaluate the influence of the single nucleotide polymorp... This study sought to explore the relationship between cytochrome P450 2C19(CYP2C19)*2/*3 polymorphisms and the development of coronary heart disease(CHD),and to evaluate the influence of the single nucleotide polymorphisms(SNPs)on the occurrence of adverse clinical events in CHD patients.A total of 231 consecutive patients candidate for percutaneous coronary intervention genotyped for CYP2C19*2(681G>A)and*3(636G>A)polymorphisms were enrolled.The adverse clinical events were recorded during a follow-up period of 14 months.The incidence of CHD,according to coronary angiography,was significantly higher(P=0.025)in CYP2C19*2 carriers group.Stepwise binary logistic regression analysis revealed that among factors that potentially influenced the presence of CHD(age>60 years,gender,BMI,etc.),CYP2C19*2 carriers(OR 1.94,95%CI:1.08-3.50,P=0.028)and male gender(OR 2.74,95%CI:1.58-4.76,P=0.001)were independent predictors,which were associated with the presence of CHD.The follow-up results showed that the incidence of adverse cardiovascular events within 14 months of discharge was significantly higher in the CYP2C 19*2 carriers than in the non-carriers(21.6%vs.6.3%,P=0.019).The results of the multivariate Cox proportional hazards model showed that CYP2C19*2 loss-of-flinction was the only independent factor which predicted the coronary events during the follow-up period of 14 months(OR=3.65,95%CI:1.09-12.25,P=0.036).The adverse impact of CYP2C19*2 polymorphisms was found not only in the risk of the presence of CHD,but also in the adverse cardiovascular events in CHD patients during the follow-up period of 14 months.However the same influence was not found in CYP2C19*3 mutation in Chinese Han population. 展开更多
关键词 CYTOCHROME P450 2C19 POLYMORPHISMS coronary heart disease CLOPIDOGREL
在线阅读 下载PDF
The expression of Spodoptera exigua P450 and UGT genes: tissue specificity and response to insecticides
5
作者 Bo Hu Shu-Heng Zhang +4 位作者 Miao-Miao Ren Xiang-Rui Tian Qi Wei David Kibe Mburu Jian-Ya Su 《昆虫科学:英文版》 SCIE CAS CSCD 2019年第2期199-216,共18页
Cytochrome P450 and UDP-glucosyltransferase (UGT) as phase I and phase II metabolism enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics. Insects can in crease the expression of det... Cytochrome P450 and UDP-glucosyltransferase (UGT) as phase I and phase II metabolism enzymes, respectively, play vital roles in the breakdown of endobiotics and xenobiotics. Insects can in crease the expression of detoxificatio n enzymes to cope with the stress from xenobiotics including insecticides. However, the molecular mechanisms for insecticide detoxification in Spodoptera exigua remain elusive, and the genes conferring insecticide metabolisms in this species are less well reported. In this study, 68 P450 and 32 UGT genes were identified. Phylogenetic analysis showed gene expansions in CYP3 and CYP4 clans of P450 genes and UGT33 family of this pest. P450 and UGT genes exhibited specific tissue expression patterns. Insecticide treatments in fat body cells of S. exigua revealed that the expression levels of P450 and UGT genes were significantly influenced by challenges of abamectin, lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb. Multiple genes for detoxification were affected in expression levels after insecticide exposures. The results demonstrated that lambda-cyhalothrin, chlorantraniliprole, metaflumizone and indoxacarb induced similar responses in the expression of P450 and UGT genes in fat body cells;eight P450 genes and four UGT genes were co-up-regulated significantly, and no or only a few CYP/UGT genes were down-regulated significantly by these four insecticides. However, abamectin triggered a distinct response for P450 and UGT gene expression;more P450 and UGT genes were down-regulated by abamectin than by the other four compounds. In con elusion, P450 and UGT genes from S. exigua were identified, and different responses to abamectin suggest a different mechanism for insecticide detoxification. 展开更多
关键词 ABAMECTIN cytochrome P450 MONOOXYGENASES gene EXPRESSION insecticide Spodoptera EXIGUA UDP-glycosyltransferase
Tobacco smoking and its drug interactions with comedications involving CYP and UGT enzymes and nicotine 预览
6
作者 Naina Mohamed Pakkir Maideen 《世界药理学杂志》 2019年第2期14-25,共12页
Tobacco smoking is a global public health threat causing several illnesses including cardiovascular disease(Myocardial infarction),cerebrovascular disease(Stroke),peripheral vascular disease(Claudication),chronic obst... Tobacco smoking is a global public health threat causing several illnesses including cardiovascular disease(Myocardial infarction),cerebrovascular disease(Stroke),peripheral vascular disease(Claudication),chronic obstructive pulmonary disease,asthma,reduced female infertility,sexual dysfunction in men,different types of cancer and many other diseases.It has been estimated in 2015 that approximately 1.3 billion people smoke,around the globe.Use of medications among smokers is more common,nowadays.This review is aimed to identify the medications affected by smoking,involving Cytochrome P450(CYP)and uridine diphosphate-glucuronosyltransferases(UGTs)enzymes and Nicotine.Polycyclic aromatic hydrocarbons(PAHs)of tobacco smoke have been associated with the induction of CYP enzymes such as CYP1A1,CYP1A2 and possibly CYP2E1 and UGT enzymes.The drugs metabolized by CYP1A1,CYP1A2,CYP2E1 and UGT enzymes might be affected by tobacco smoking and the smokers taking medications metabolized by those enzymes,may need higher doses due to decreased plasma concentrations through enhanced induction by PAHs of tobacco smoke.The prescribers and the pharmacists are required to be aware of medications affected by tobacco smoking to prevent the toxicityassociated complications during smoking cessation. 展开更多
关键词 Drug Interactions Tobacco smoking CYTOCHROME P450 ENZYMES URIDINE diphosphate-glucuronosyltransferases ENZYMES NICOTINE
在线阅读 免费下载
脓毒症大鼠细胞色素水平对肝细胞线粒体功能的影响
7
作者 李坤 方雪玲 方强 《中华实验外科杂志》 CSCD 北大核心 2018年第11期2062-2065,共4页
目的观察脓毒症大鼠细胞色素水平和细胞色素C氧化酶活性对肝细胞线粒体生物能量合成和肝脏功能的影响。方法将SD大鼠24只,分为正常对照组(C组)、脓毒症组(S组),每组12只。采用盲肠结扎穿孔(CLP)法建立大鼠脓毒症模型。制模18h... 目的观察脓毒症大鼠细胞色素水平和细胞色素C氧化酶活性对肝细胞线粒体生物能量合成和肝脏功能的影响。方法将SD大鼠24只,分为正常对照组(C组)、脓毒症组(S组),每组12只。采用盲肠结扎穿孔(CLP)法建立大鼠脓毒症模型。制模18h后处死大鼠,取血检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和天冬氨酸氨基转移酶线粒体同工酶(m-AST),使用光镜和电子显微镜分别观察肝细胞和线粒体形态,采用还原-氧化差光谱法测定线粒体细胞色素C、C1、B和AA3含量,测定细胞色素C氧化酶活性,检测线粒体膜电位水平,测定肝细胞三磷酸腺苷(ATP)含量。结果与C组比较,S组血清ALT[(233.02±110.16)U/L]、AST[(742.56±441.41)U/L]和m-AST[(412.78±252.56)U/L]明显升高[C组相应值为(29.81±13.16)、(99.78±41.67)、(46.23±24.24)U/L,P=0.000、0.001、0.001],肝细胞ATP含量[(19.27±8.76)nmol/mg]明显减少[C组(94.65±17.79)nmol/mg,P=0.000],线粒体中细胞色素C[(0.104±0.04)nmol/mg]和AA3[(0.21±0.04)nmo]/mg]明显下降[C组相应值为(0.17±0.03)、(0.38±0.09)nmoL/mg,P=0.000],细胞色素c氧化酶活性[(734.14±107.37)amol/(min·mgprot)]明显下降[c组(1279.75±186.77)nmol/(min·mg prot),P=0.000],线粒体膜电位水平(1.75±0.96)明显下降(C组:3.81±1.16,P=0.000)。结论脓毒症时肝细胞线粒体细胞色素含量减少和细胞色素c氧化酶活性下降,可引起线粒体功能障碍和急性肝细胞搠伤。 展开更多
关键词 脓毒症 肝脏 线粒体 细胞色素 细胞色素C氧化酶
小麦细胞色素P450基因克隆及其序列研究 预览
8
作者 高璇 《生物化工》 2018年第5期37-39,共3页
细胞色素P450广泛存在于生物界中,它是一种含亚铁血红素单加氧酶,对多种生物的防御反应起到重要的作用。本文研究使用RACE方式,从普通小麦中克隆出新的P450基因,并将其命名为TAP450。这种新基因的基因组序列和普通的P450不同,全长2... 细胞色素P450广泛存在于生物界中,它是一种含亚铁血红素单加氧酶,对多种生物的防御反应起到重要的作用。本文研究使用RACE方式,从普通小麦中克隆出新的P450基因,并将其命名为TAP450。这种新基因的基因组序列和普通的P450不同,全长2643bp,有2个外显子和1个内含子蛋白序列和已知的P450蛋白的差异性较大。蛋白分子量是57.092kD。这种新的P450基因在小麦的叶片、茎和种子中都存在,胁迫处理下,这种基因的表达量上调。 展开更多
关键词 小麦 细胞色素 P450 基因克隆
在线阅读 下载PDF
Food restriction decreases BMR,body and organ mass,and cellular energetics,in the Chinese Bulbul(Pycnonotus sinensis) 预览
9
作者 Yiying Zhang Ke Yang +3 位作者 Peipei Yang Yingshi Su Weihong Zheng Jinsong Liu 《鸟类学研究:英文版》 CSCD 2018年第4期370-380,共11页
Background:Food is an important environmental factor that affects animals’energy metabolism and food shortage has significant effects on animals’behavior,physiology and biochemistry.However,to date few studies have ... Background:Food is an important environmental factor that affects animals’energy metabolism and food shortage has significant effects on animals’behavior,physiology and biochemistry.However,to date few studies have focused on the thermogenesis and its effects on the body condition of birds.In this study,we examined the effects of food restriction on the body mass,basal metabolic rate(BMR)and body composition,and several physiological,biochemical and molecular markers potentially related to thermogenesis,in the Chinese Bulbul(Pycnonotus sinensis).Methods:Birds in the control group were provided with food ad libitum whereas those in the food restriction group were provided with one-half of the usual quantity of food for 12 days.Oxygen consumption was measured using an open-circuit respirometry system.Mitochondrial state 4 respiration and cytochrome c oxidase(COX)activity in the liver and pectoral muscle were measured with a Clark electrode.Avian uncoupling protein(avUCP)mRNA expression was determined in pectorals muscle with quantitative Real-time PCR.Results:Chinese Bulbuls in food restriction group decreased in body mass,BMR and internal organ(heart,kidneys,small intestine and total digestive tract)mass compared with the control group over the 12-day period of food restriction.Bulbuls in the food restriction group also had lower levels of state-4 respiration,COX activity in the liver and muscle,and mitochondrial avUCP gene expression in muscle compared to the control group.BMR was positively correlated with body mass,state 4 respiration in the liver and COX activity in the muscle.Conclusions:Our data indicate that Chinese Bulbuls not only sustain food shortage through simple passive mechanisms,such as reducing body and organ mass and energy expenditure,but also by reducing energetic metabolism in the liver and muscle. 展开更多
关键词 Avian UNCOUPLING protein BASAL metabolic rate CYTOCHROME c OXIDASE Food restriction Pycnonotus sinensis State-4 RESPIRATION
在线阅读 免费下载
Morphological and molecular characterization of the rice root-knot nematode,Meloidogyne graminicola,Golden and Birchfeild,1965 occurring in Zhejiang,China 预览
10
作者 TIAN Zhong-ling Munawar Maria +2 位作者 Eda Marie Barsalote Pablo Castillo ZHENG Jing-wu 《农业科学学报:英文版》 SCIE CAS CSCD 2018年第12期2724-2733,共10页
The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nemato... The rice root-knot nematode Meloidogyne graminicola is a severe pest of rice. In China, it was first reported from Hainan Province, and later from several other provinces. In the present study, a rice root-knot nematode population found from the rice cultivation areas of Zhejiang Province, China is characterized via molecular analysis using internal transcribed spacer(ITS) and cytochrome c oxidase subunit Ⅱ(coxⅡ)-16 S rRNA genes and scanning electron microscopy(SEM) observations of males and the second-stage juveniles. Morphometric data and molecular sequence comparisons for all M. graminicola populations occurring in China are also provided. The overall morphology of M. graminicola found in Zhejiang match well with the original description, though males have a slightly longer body and stylet, and a shorter tail, while the second-stage juvenile is also slightly longer than in the original description. This is the first report of M. graminicola from Zhejiang. Phylogenetic studies based on coxⅡ suggest that all the Chinese populations belong to Type B. This study expands knowledge of the increasing distribution and phylogenetic relationships of M. graminicola that occur in China. 展开更多
关键词 MELOIDOGYNE graminicola morphology MORPHOMETRIC molecular RICE rDNA CYTOCHROME c OXIDASE SUBUNIT (coxⅡ) China
在线阅读 下载PDF
草甘膦对铜绿微囊藻生长和叶绿素荧光诱导动力学的影响 预览
11
作者 潘文静 王钰亮 +1 位作者 刘洋 李效宇 《河南师范大学学报:自然科学版》 北大核心 2018年第4期89-94,共6页
以铜绿微囊藻Microcystis aeruginosa PCC7806为研究对象,研究草甘膦Glyphosate胁迫下M.aeruginosa PCC7806生长、细胞色素含量的变化,以及对光合系统的影响.结果表明,当草甘膦质量浓度50mg/L时,M.aeruginosa PCC7806的生长受到显著抑... 以铜绿微囊藻Microcystis aeruginosa PCC7806为研究对象,研究草甘膦Glyphosate胁迫下M.aeruginosa PCC7806生长、细胞色素含量的变化,以及对光合系统的影响.结果表明,当草甘膦质量浓度50mg/L时,M.aeruginosa PCC7806的生长受到显著抑制.通过线性回归分析,96h,草甘膦对铜绿微囊藻的半效应质量浓度EC50为62.33mg/L.草甘膦质量浓度为200mg/L时能显著降低铜绿微囊藻单位细胞内叶绿素a和类胡萝卜素含量.光诱导动力学分析结果显示,低质量浓度下光系统没有表现出明显抑制,并表现出高于对照组的峰,而当质量浓度为100mg/L时,24h与96h结果发现O-J-I-P特征均已消失,表明其光合系统Ⅱ(PSⅡ)活性受到抑制. 展开更多
关键词 草甘膦 细胞色素 铜绿微囊藻 半效应质量浓度EC50 叶绿素荧光
在线阅读 免费下载
CYP2C19 polymorphism has no influence on rabeprazolebased hybrid therapy for Helicobacter pylori eradication 预览
12
作者 Tsung-Jung Lin Hsi-Chang Lee +3 位作者 Chih-Lin Lin Chung-Kwe Wang Kuan-Yang Chen Deng-Chyang Wu 《世界临床病例杂志》 2018年第12期514-520,共7页
AIM To evaluate the impact of cytochrome P450 2C19(CYP2C19)and interleukin-1β(IL-1β)polymorphisms on the efficacy of Helicobacter pylori(H.pylori)eradication by using rabeprazole-based hybrid therapy.METHODS A total... AIM To evaluate the impact of cytochrome P450 2C19(CYP2C19)and interleukin-1β(IL-1β)polymorphisms on the efficacy of Helicobacter pylori(H.pylori)eradication by using rabeprazole-based hybrid therapy.METHODS A total of 88 H.pylori-infected patients were recruited to receive 14-d of hybrid therapy from March 2013 to May 2014.Three patients were excluded from analysis because of incomplete compliance.Either a follow-up endoscopy or 13C-urea test was performed to determine the results of H.pylori eradication therapy.The genotypes of CYP2C19 and IL-1βwere analyzed to investigate the impact on treatment effect.RESULTS The total eradication rate of H.pylori was 92.94%(79/85).According to the CYP2C19 genotypes,the rates of H.pylori eradication were 89.19%in extensive metabolizers(EM)and 95.83%in non-EM.The H.pylori eradication rates regarding the IL-1βgenotypes were 92.59%in the normal acid secretion group and 93.10%in the low acid secretion group.After multivariable logistic regression analysis,both the genotypes of CYP2C19 and IL-1βhad no significant influences on the eradication rates of H.pylori.CONCLUSION The CYP2C19 and IL-1βpolymorphisms are not significantly independent factors of H.pylori eradication using rabeprazole-based hybrid therapy. 展开更多
关键词 HELICOBACTER PYLORI CYTOCHROME P450 2C19 INTERLEUKIN-1Β Hybrid therapy RABEPRAZOLE
在线阅读 免费下载
甲磺隆对微囊藻生长和光合系统的影响 预览
13
作者 马晓洁 王钰亮 +3 位作者 卢韩杨 刘洋 李效宇 李仁辉 《水生生物学报》 CSCD 北大核心 2018年第4期839-845,共7页
研究选取了水体常见蓝藻优势种类——铜绿微囊藻(Microcystis aeruginosa PCC7806)作为研究对象,了解磺酰脲类除草剂甲磺隆(Metsulfuron-methyl)对铜绿微囊藻生长和光合系统的影响。研究表明,当甲磺隆浓度大于80 mg/L时,对铜绿微囊藻的... 研究选取了水体常见蓝藻优势种类——铜绿微囊藻(Microcystis aeruginosa PCC7806)作为研究对象,了解磺酰脲类除草剂甲磺隆(Metsulfuron-methyl)对铜绿微囊藻生长和光合系统的影响。研究表明,当甲磺隆浓度大于80 mg/L时,对铜绿微囊藻的生长具有显著抑制。通过回归分析和Probit分析,甲磺隆对铜绿微囊藻生长的EC50为81.998 mg/L。细胞色素研究结果显示,实验第6天,各浓度处理下单位细胞内Chl.a和类胡萝卜素含量均低于对照组,且当甲磺隆浓度为80 mg/L时,单位细胞内类胡萝卜素含量显著低于对照组。快速叶绿素荧光诱导动力学变化结果分析显示,实验第6天甲磺隆胁迫下单位反应中心捕获的用于电子传递的能量(ET0/RC)及单位反应中心用于电子传递的量子产额(φE0)受到显著抑制,综合细胞色素变化结果显示,甲磺隆能显著抑制光合系统反应中心电子受体侧电子性能。综上所述,甲磺隆可能作用于光合系统反应中心电子受体侧,从而对铜绿微囊藻光合系统造成影响。 展开更多
关键词 甲磺隆 铜绿微囊藻 叶绿素诱导荧光 细胞色素
在线阅读 下载PDF
三倍体雌性虹鳟性腺发育阶段细胞色素相关基因的表达 预览
14
作者 相福生 徐革锋 +3 位作者 谷伟 黄天晴 刘晨斌 王炳谦 《中国水产科学》 CSCD 北大核心 2018年第5期949-957,共9页
Cyp19a1b、Cyp11a1、Cyp11b2、Cyp17a1、Hsd3b1等细胞色素相关基因能够调节硬骨鱼类性类固醇的合成,对性腺发育和性别决定产生影响。本研究以全雌三倍体虹鳟(Oncorhynchus mykiss)为研究对象,正常雌性二倍体虹鳟为对照,选取31~68 dpf(da... Cyp19a1b、Cyp11a1、Cyp11b2、Cyp17a1、Hsd3b1等细胞色素相关基因能够调节硬骨鱼类性类固醇的合成,对性腺发育和性别决定产生影响。本研究以全雌三倍体虹鳟(Oncorhynchus mykiss)为研究对象,正常雌性二倍体虹鳟为对照,选取31~68 dpf(days post fertilization)时间段的虹鳟仔鱼脑组织,采用qRT-PCR和酶联免疫的方法研究以上几种基因的表达状况和脑芳香化酶的活性变化,以期探明导致三倍体雌性虹鳟性腺发育异常的关键原因。qRTPCR结果显示,二倍体中Cyp19a1b在30~50 dpf时表达量上调并且维持在较稳定水平,但50~56 dpf时表达量逐渐下调,之后56~68 dpf表达量持续上调;三倍体中Cyp19a1b表达量在30~35 dpf开始上调,35~47 dpf逐渐下调,47~55 dpf开始第二次上调,之后维持在较稳定水平直至68 dpf,但三倍体Cyp19a1b的表达量显著(P<0.05)低于同期二倍体的。二倍体Cyp11a1表达量在34 dpf出现峰值,三倍体Cyp11a1在38 dpf时出现峰值。二倍体Hsd3b1表达量在33~42 dpf时维持在较高水平,在38 dpf时出现高峰;三倍体Hsd3b1表达量在47~59 dpf时较高,在49 dpf出现高峰。二倍体中Cyp11b2在37 dpf出现峰值,之后开始下调;三倍体在40 dpf出现峰值,之后逐渐下调,但三倍体Cyp11b2表达量显著低于同期二倍体。二倍体Cyp17a1的表达量在35~46 dpf时逐渐上升,在45 dpf时达到高峰之后直至69 dpf逐渐下降,并且维持在较为平稳的水平上;但是在相同的实验条件下未检测到同一时期三倍体Cyp17a1的表达量。酶联免疫结果显示,在40 dpf时二者的脑芳香化酶活性到达高峰,但在40~60 dpf时期,二倍体虹鳟脑芳香化酶活性显著(P<0.05)高于三倍体虹鳟,尤其在45~50 dpf时,该酶活性分别较三倍体的高1.15倍和1.12倍。以上结果表明三倍体虹鳟早期性腺发育迟缓的原因之一是Cyp19a1b、Cyp11a1、Cyp11b2、Cyp17a1、Hsd3b1等基因的表达晚于二倍体,且表达量低于二倍体,造成雌二醇不能正常合� 展开更多
关键词 虹鳟 性腺发育 脑芳香化酶 细胞色素
在线阅读 下载PDF
Synthesis,biochemical evaluation and computational simulations of new cytochrome bc1 complex inhibitors based on N-(4-aryloxyphenyl)phthalimides
15
作者 Hua Cheng Yan Fu +6 位作者 Qing Chang Ni Zhang Mengwei Bu Yan Niu Qiongyou Wu Cheng Chen Francis Verpoort 《中国化学快报:英文版》 SCIE CAS CSCD 2018年第12期1897-1900,共4页
The cytochrome bc1 complex(the bc1 complex or complex Ⅲ) is an attractive target for the discovery of numerous pharmaceuticals and pesticides. In order to identify new lead structures for this target, a new series of... The cytochrome bc1 complex(the bc1 complex or complex Ⅲ) is an attractive target for the discovery of numerous pharmaceuticals and pesticides. In order to identify new lead structures for this target, a new series of molecules, N-(4-aryloxyphenyl)phthalimides, were designed and synthesized in a straightforward manner. Our design strategy was to introduce a 4-aryloxyphenyl group, a fragment which exhibited promising bc1 complex-inhibiting properties, into the aryl group of the valuable N-arylphthalimide backbone. Afterward, the biochemical evaluation of the newly synthesized compounds was carried out,and the results implied that several compounds demonstrated good activities against succinatecytochrome reductase(SCR, a mixture of mitochondrial complex Ⅱ and the bc1 complex). Further studies confirmed that 3e’, a representative compound in this paper, was identified as an inhibitor of the bc1 complex. Furthermore, computational simulations were also performed to better understand binding of 3e’ to the enzyme complex, which indicated that 3e’ should bind to the Qo site of the bc1 complex.Consequently, we harbor the idea that this paper can provide a solid platform for synthesis and discovery of other bc1 complex inhibitors. 展开更多
关键词 Cytochrome bc1 complex Inhibitor N-(4-Aryloxyphenyl)phthalimide SYNTHESIS BIOCHEMICAL EVALUATION Computational simulation
线粒体调控细胞凋亡的相关研究综述 预览
16
作者 黄兆宇 武睿超 +6 位作者 冉江华 刘钧汉 张黎 郑克谱 李望 周焘 张熙冰 《吉林医学》 CAS 2018年第7期1356-1357,共2页
细胞凋亡是指为维持内环境的稳定,由基因调控的细胞自主、有序的死亡,又称程序性细胞死亡。细胞凋亡是一件主动的过程,它涉及一系列基因的激活、表达以及调控等过程,是为了更好适应生存环境而主动采取的一种死亡过程。它在生物体的进化... 细胞凋亡是指为维持内环境的稳定,由基因调控的细胞自主、有序的死亡,又称程序性细胞死亡。细胞凋亡是一件主动的过程,它涉及一系列基因的激活、表达以及调控等过程,是为了更好适应生存环境而主动采取的一种死亡过程。它在生物体的进化、内环境的稳定以及多个系统的发育中起着重要作用,且凋亡过程的紊乱可能与许多疾病的发生有直接或间接的关系。因此,有关细胞凋亡过程的研究,已成为生物学及医学研究的一个热点,引起越来越多人关注。 展开更多
关键词 线粒体 细胞凋亡 CYTOCHROME C AIF MPTP BCL-2
在线阅读 下载PDF
Identification of the 2-tridecanone cis-acting element in the promoter of cytochrome P450 CYP6B7 in Helicoverpa armigera
17
作者 Li Xu Dong-Zhi Li +2 位作者 Yuan-Yuan Luo Jian-Ying Qin Li-Hong Qiu 《昆虫科学:英文版》 SCIE CAS CSCD 2018年第6期959-968,共10页
The expression level of cytochrome P450 genes in insects can be induced by plant allelochemicals,which is important for insects to adapt to host plants.Cytochrome P450CYP6B 7has been reported to be involved in pyethro... The expression level of cytochrome P450 genes in insects can be induced by plant allelochemicals,which is important for insects to adapt to host plants.Cytochrome P450CYP6B 7has been reported to be involved in pyethroid insecticide resistance in Heli- coverpa armigera,and its transcription level was induced by some inducers.Currently,the regulatory mechanism of the induced expression of CYP6B7remains unknown,although it is very important for understanding the detoxification mechanism to allelochemicals in host plants.The objective of the present study was to investigate the eis-acting ele- ment in the promoter of CYP6B7 mediating the inducible up-regulation of CYP6B7in H.armigera by 2-tridecanone.The promoter region of CYP6B7was cloned by genome walking technique and analyzed by transient transfeetion assay.Progressive 5'deletion of the promoter region of CYP6B7revealed that the relative luciferase activity of construct -320/+232could be significantly induced by 2-trideeanone.Further stepwise deletion between -320 and -238 bp found that construct -292/+232 could also be significantly induced by 2-tridecanone,but the adjacent construct -256/+232could not,suggesting the essential role of the sequence between -292 and --257 bp for 2-tridecanone induction. Nucleotide mutations between -292 and -281 bp had no influence on the induction ef- fect by 2-tridecanone,but nucleotide mutations between -280 and -257 bp significantly decreased the induction effect.These results demonstrated that the cis-acting element for 2-trideeanone induction was between -280 and -257 bp in the promoter of CYP6B7. 展开更多
关键词 cis-acting element CYTOCHROME P450 CYP6B7 HELICOVERPA ARMIGERA induction 2-tridecanone
Pb、Cu胁迫对玉米(Zeamays L.)生长、细胞色素合成以及重金属吸收特性的影响 被引量:1
18
作者 陆干 李磊明 +2 位作者 陶祥运 刘小红 司友斌 《安徽农业大学学报》 CSCD 北大核心 2017年第5期905-911,共7页
通过水培试验,以玉米作为供试植株,研究Pb、Cu胁迫对玉米生长发育、细胞色素合成以及重金属吸收的影响。结果表明,Pb、Cu单一胁迫下玉米根长较对照组均偏低,复合胁迫下根长随着Pb、Cu复合浓度的增加而减小。玉米植株在重金属Pb、Cu... 通过水培试验,以玉米作为供试植株,研究Pb、Cu胁迫对玉米生长发育、细胞色素合成以及重金属吸收的影响。结果表明,Pb、Cu单一胁迫下玉米根长较对照组均偏低,复合胁迫下根长随着Pb、Cu复合浓度的增加而减小。玉米植株在重金属Pb、Cu单一胁迫下生物量变化不明显,但在不同水平Pb、Cu复合胁迫下,表现随着重金属胁迫浓度增加,玉米植株生物量先增加后减小。玉米叶绿素含量随着Pb胁迫浓度增加而增加、随着Cu浓度增加而降低;Pb、Cu单一胁迫下β-类胡萝卜素含量随着Pb浓度增加先降低后增加,随着Cu含量增加先增加后降低;Pb、Cu单一胁迫下花青素含量较对照组均增大,Cu胁迫下花青素含量增加更明显,最低增加0.65%,最高增加21.25%。玉米不同器官对Pb、Cu积累有一定差异,在单一重金属胁迫下Pb、Cu在玉米体内积累量与胁迫浓度呈正相关,分布顺序为根系>茎叶,根部是积累Pb、Cu主要器官。Pb、Cu复合胁迫下加剧了重金属向玉米体内的迁移累积,随着Pb、Cu复合胁迫浓度的增大,根部吸收Pb、Cu逐步增大,而茎叶吸收Pb、Cu趋于平稳。相关分析显示,玉米细胞色素的合成取决于被胁迫的重金属类型,β-类胡萝卜素含量与植株体内Pb含量显著正相关,花青素含量与Cu含量显著正相关, 揭示Pb对β-类胡萝卜素诱导作用强、Cu对花青素诱导作用强。 展开更多
关键词 重金属 玉米 细胞色素 吸收特性
CYP1A1、CYP2E1、PON2148、MTHFR基因多态性与低出生体重儿易感性的meta分析
19
作者 王艳杰 杨蕾 +2 位作者 木尼热·热孜 艾克然木·艾合买提 刘早玲 《职业与健康》 CAS 2017年第11期1482-1485,共4页
目的探讨CYP1A1、CYP2E1、PON2148、MTHFR基因多态性与低出生体重儿(LBW)易感性的关系。方法根据纳入排除标准全面检索万方、Pub Med、Web of Science、EMBASE、中国生物医学文献数据库和CNKI等数据库相关的文献,收集包括对氧磷酶基因... 目的探讨CYP1A1、CYP2E1、PON2148、MTHFR基因多态性与低出生体重儿(LBW)易感性的关系。方法根据纳入排除标准全面检索万方、Pub Med、Web of Science、EMBASE、中国生物医学文献数据库和CNKI等数据库相关的文献,收集包括对氧磷酶基因(PON2 148),细胞色素(CYP1A1、CYP2E1),亚甲基四氢叶酸还原酶(MTHFR)基因与LBW的关联性的研究。应用Revman 5.3软件,按照文献的异质性,采用固定效应模型或随机效应模型对数据进行meta分析。结果共纳入7篇文献进行meta分析,其中中文6篇,英文1篇。关于PON2 148与低出生体重儿的文献3篇,MTHFR与低出生体重儿的文献2篇,CYP1A1、CYP2E1与低出生体重儿的文献4篇。母亲携带PON2 148基因型Gly148Gly/Ala148Gly新生儿低出生体重的关联差异无统计学意义(OR=1.35,95%CI:0.86~2.13,P=0.19);母亲携带MTHFR基因型CT/TT新生儿低出生体重的关联差异无统计学意义(OR=1.19,95%CI:0.80~1.76,P=0.40);母亲携带CYP1A1基因多态性与低出生体重的关联差异无统计学意义(OR=1.55,95%CI:0.92~2.62,P=0.10);母亲携带CYP2E1基因多态性与低出生体重的关联差异有统计学意义(OR=1.67,95%CI:1.03~2.72,P=0.04)。新生儿携带CYP2E1基因多态性与低出生体重儿的关联差异无统计学意义(OR=1.47,95%CI:0.93~2.33,P=0.10)。结论母亲CYP1A1、CYP2E1、PON2 148、MTHFR基因和新生儿CYP1A1、CYP2E1基因是新生儿低出生体重发生的危险因素。 展开更多
关键词 对氧磷酶 亚甲基四氢叶酸还原酶 细胞色素基因 基因多态性 低出生体重儿 META分析
参芎葡萄糖注射液对肝脏6种亚型微粒体细胞色素P450酶的抑制作用 预览 被引量:3
20
作者 孙佳 陆苑 +4 位作者 潘洁 郑林 黄勇 王永林 李勇军 《贵阳医学院学报》 CAS 2016年第7期775-778,共4页
目的:考察参芎葡萄糖注射液对人肝微粒体细胞色素P450(CYP450)酶6种亚型CYP1A2、CYP2E1、CYP2C9、CYP2C19、CYP2D6、CYP3A4的体外抑制作用。方法:在人肝微粒体孵育系统中,将不同浓度的参芎葡萄糖注射液和7种混合探针底物(非那西... 目的:考察参芎葡萄糖注射液对人肝微粒体细胞色素P450(CYP450)酶6种亚型CYP1A2、CYP2E1、CYP2C9、CYP2C19、CYP2D6、CYP3A4的体外抑制作用。方法:在人肝微粒体孵育系统中,将不同浓度的参芎葡萄糖注射液和7种混合探针底物(非那西丁、氯唑沙宗、甲苯磺丁脲、奥美拉唑、右美沙芬、睾酮和咪达唑仑)共同孵育,采用超高效液相色谱-串联质谱法( UPLC-MS/MS)同时测定孵育液中 CYP1A2、CYP2E1、CYP2C9、CYP2C19、CYP2D6和CYP3A4酶对应代谢物(对乙酰氨基酚、6-羟基氯唑沙宗、羟基甲苯磺丁脲、5-羟基奥美拉唑、右啡烷、6β-羟基睾酮和α-羟基咪达唑仑)的剩余量,与对照组进行比较,得到酶的剩余活性,用 GraphPad v5.0软件计算半抑制浓度(IC_50)值,考察参考葡萄糖注射液对人肝微粒体CYP450酶的抑制程度。结果:在人肝微粒体孵育体系中,参芎葡萄糖注射液对6种CYP450亚型酶的Ic50均〉15%。结论:在临床剂量下,参芎葡萄糖注射液对人肝微粒体CYPlA2、CYP2E1、CYP2C9、CYP2C19、CYP2D6和CYP3A4无明显抑制作用。 展开更多
关键词 参考葡萄糖注射液 细胞色素P450 微粒体 药物相互作用 CYTOCHROME P450
在线阅读 下载PDF
上一页 1 2 220 下一页 到第
使用帮助 返回顶部 意见反馈