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Comparison of Five Endogenous Reference Genes for Specific PCR Detection and Quantification of Rice 预览
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作者 ZHANG Xiujie JIN Wujun +4 位作者 XU Wentao LI Xiaying SHANG Ying LI Sha OUYANG Hongsheng 《水稻科学:英文版》 CSCD 2019年第4期248-256,I0006,I0007共11页
Endogenous reference genes (ERGs) provide vital information regarding genetically modified organisms (GMOs). The successful detection of ERGs can identity GMOs and the source of genes, verify stability and reliability... Endogenous reference genes (ERGs) provide vital information regarding genetically modified organisms (GMOs). The successful detection of ERGs can identity GMOs and the source of genes, verify stability and reliability of the detection system, and calculate the level of genetically modified (GM) ingredients in mixtures. The reported ERGs in rice include sucrose-phosphate synthase (SPS), phospholipase D (PLD), RBE4 and rice root-specific GOS9 genes. Based on the characteristics of ERGs, a new ERG gene, phosphoenolpyruvate carboxylase (PEPC), was selected, and further compared with the four existing genes. A total of 18 rice varieties and 29 non-rice crops were used to verify the interspecies specificity, intraspecies consistency, sensitivity, stability and reliability of these five ERGs using qualitative and quantitative PCR. Qualitative detection indicated that SPS and PEPC displayed sufficient specificity, and the detection sensitivity was 0.05% and 0.005%, respectively. Although the specificity of both RBE4 and GOS9 were adequate, the amplicons were small and easily confused with primer dimers. Non-specific amplification of the PLD gene was present in maize and potato. Real-time quantitative PCR detection indicated that PLD, SPS and PEPC displayed good specificity, with R2 of the standard curve greater than 0.98, while the amplification efficiency ranged between 90% and 110%. Both the detection sensitivities of PLD and PEPC were five copies and that of SPS was ten copies. RBE4 showed typical amplification in maize, beet and Arabidopsis, while GOS9 was found in maize, tobacco and oats. PEPC exhibited excellent detection sensitivity and species specificity, which made it a potentially useful application in GM-rice supervision and administration. Additionally, SPS and PLD are also suitable for GM-rice detection. This study effectively established a foundation for GMO detection, which not only provides vital technical support for GMO identification, but also is of great significance for enhancing the comparability o 展开更多
关键词 ENDOGENOUS reference GENE RICE genetically modified crop PHOSPHOENOLPYRUVATE CARBOXYLASE GENE sucrose-phosphate synthase GENE phospholipase D GENE starch branching enzyme 4 GENE RICE root-specific GOS9 GENE
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Harnessing the potential of gene editing technology using CRISPR in inflammatory bowel disease 预览
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作者 Viktor Limanskiy Arpita Vyas +1 位作者 Lakshmi Shankar Chaturvedi Dinesh Vyas 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第18期2177-2187,共11页
The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel ... The molecular scalpel of clustered regularly interspersed short palindromic repeats/CRISPR associated protein 9 (CRISPR/Cas9) technology may be sharp enough to begin cutting the genes implicated in inflammatory bowel disease (IBD) and consequently decrease the 6.3 billion dollar annual financial healthcare burden in the treatment of IBD. For the past few years CRISPR technology has drastically revolutionized DNA engineering and biomedical research field. We are beginning to see its application in gene manipulation of sickle cell disease, human immunodeficiency virus resistant embryologic twin gene modification and IBD genes such as Gatm (Glycine amidinotransferase, mitochondrial), nucleotide-binding oligomerization domain-containing protein 2, KRT12 and other genes implicated in adaptive immune convergence pathways have been subjected to gene editing, however there are very few publications. Furthermore, since Crohn’s disease and ulcerative colitis have shared disease susceptibility and share genetic gene profile, it is paramount and is more advantageous to use CRISPR technology to maximize impact. Although, currently CRISPR does have its limitations due to limited number of specific Cas enzymes, off-target activity, protospacer adjacent motifs and crossfire between different target sites. However, these limitations have given researchers further insight on how to augment and manipulate enzymes to enable precise gene excision and limit crossfire between target sites. 展开更多
关键词 Clustered regularly interspersed short palindromic REPEATS INFLAMMATORY BOWEL DISEASE Crohn’s DISEASE Ulcerative colitis GENE excision GENE EDITING GENE therapy Financial impact of INFLAMMATORY BOWEL DISEASE on healthcare Clustered regularly interspersed short palindromic REPEATS crossfire
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Polymorphism analysis of virulence-related genes among Candida tropicalis isolates
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作者 Li-Juan Zhang Shuan-Bao Yu +3 位作者 Wen-Ge Li Wen-Zhu Zhang Yuan WU Jin-Xing Lu 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第4期446-453,共8页
Background: Adhesion, biofilm formation, yeast-hyphal transition, secretion of enzymes, and hemolytic activity are all considered important factors in Candida tropicalis infection. However, DNA sequence data for this ... Background: Adhesion, biofilm formation, yeast-hyphal transition, secretion of enzymes, and hemolytic activity are all considered important factors in Candida tropicalis infection. However, DNA sequence data for this pathogen are limited. In this study, the polymorphism and heterogeneity of genes agglutinin-like sequences (ALS)2, Lipase (LIP)1, LIP4, and secretory aspartyl proteinase tropicalis (SAPT)1-4 as well as the relationship between phenotype and genotype were analyzed. Methods: This study started in August 2013, and ended in July 2017. The complete length of ALS2, LIP1, LIP4, and SAPT1-4 of 68 clinical C. tropicalis isolates was sequenced. Single nucleotide polymorphisms (SNPs) as well as insertions and deletions (indels) were identified within these genes. In addition, phenotypic characteristics of the virulent factors, including adhesion and the secretion of aspartyl proteinases and phospholipases, were determined. Results: There were 73, 24, 17, 16, 13, and 180 SNPs in the genes LIP1, LIP4, SAPT1, SAPT2, SAPT3, and SAPT4, respectively. Furthermore, 209 SNPs were identified in total for the gene ALS2. Interestingly, large fragment deletions and insertions were also found in ALS2. Isolate FXCT 01 obtained from blood had deletions on all 4 sites and showed the lowest adhesion ability on the polymethylpentene surface. In addition, isolates with deletions in the regions 1697 to 1925 and 2073 to 2272 bp displayed relatively low abilities for adhesion and biofilm formation, and this phenotype correlated with the deletions found in ALS2. LIP1, SAPT4, and ALS2 displayed great heterogeneity among the isolates. Large deletions found in gene ALS2 appeared to be associated with the low ability of adhesion and biofilm formation of C. tropicalis. Conclusion: This study might be useful for deeper explorations of gene function and studying the virulent mechanisms of C. tropicalis. 展开更多
关键词 CANDIDA TROPICALIS Virulence-related GENES PHYLOGENETIC analysis GENE ALS GENE LIP GENE SAP
体育活动与心理健康状况关系的基因假说 预览
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作者 艾水 梁琴 《四川文理学院学报》 2019年第3期126-132,共7页
体育活动与心理健康水平之间存在着显著的相关关系,以至于绝大多数研究都认为体育活动是促进心理健康水平的重要原因。然而,近期生物遗传学尤其是双生子的研究认为:体育活动对心理健康的影响可能是由于被试的基因型决定的,提出了两种影... 体育活动与心理健康水平之间存在着显著的相关关系,以至于绝大多数研究都认为体育活动是促进心理健康水平的重要原因。然而,近期生物遗传学尤其是双生子的研究认为:体育活动对心理健康的影响可能是由于被试的基因型决定的,提出了两种影响二者间关系的机制,即基因的多效性和基因与体育活动的交互作用,那些影响体育活动的基因和影响心理健康水平的基因可能存在着部分重合。 展开更多
关键词 体育活动 基因 心理健康 基因多效性 基因-体育活动交互作用
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Topological evolution of coexpression networks by new gene integration maintains the hierarchical and modular structures in human ancestors
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作者 Jian Zu Yuexi Gu +6 位作者 Yu Li Chentong Li Wenyu Zhang Yong E.Zhang UnJin Lee Li Zhang Manyuan Long 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第4期594-608,共15页
We analyze the global structure and evolution of human gene coexpression networks driven by new gene integration.When the Pearson correlation coefficient is greater than or equal to 0.5,we find that the coexpression n... We analyze the global structure and evolution of human gene coexpression networks driven by new gene integration.When the Pearson correlation coefficient is greater than or equal to 0.5,we find that the coexpression network consists of 334 small components and one "giant" connected subnet comprising of 6317 interacting genes.This network shows the properties of power-law degree distribution and small-world.The average clustering coefficient of younger genes is larger than that of the elderly genes(0.6685 vs.0.5762).Particularly,we find that the younger genes with a larger degree also show a property of hierarchical architecture.The younger genes play an important role in the overall pivotability of the network and this network contains few redundant duplicate genes.Moreover,we find that gene duplication and orphan genes are two dominant evolutionary forces in shaping this network.Both the duplicate genes and orphan genes develop new links through a "rich-gets-richer"mechanism.With the gradual integration of new genes into the ancestral network,most of the topological structure features of the network would gradually increase.However,the exponent of degree distribution and modularity coefficient of the whole network do not change significantly,which implies that the evolution of coexpression networks maintains the hierarchical and modular structures in human ancestors. 展开更多
关键词 NETWORK biology GENE NETWORK EVOLUTION SCALE-FREE NETWORK natural selection GENE expression self-organization gene DUPLICATION
维生素D受体基因多态性与骨质疏松症相关性的研究进展 预览
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作者 李明 李宁宁 《右江医学》 2019年第4期245-249,共5页
骨质疏松症的发生是遗传因素和环境因素共同作用的结果,其终末阶段是骨质疏松性骨折。随着分子生物学的发展和疾病遗传学的深入研究,骨质疏松症相关基因的多态性成为目前研究的热点。维生素D受体基因是目前研究最多也是最有争议的基因... 骨质疏松症的发生是遗传因素和环境因素共同作用的结果,其终末阶段是骨质疏松性骨折。随着分子生物学的发展和疾病遗传学的深入研究,骨质疏松症相关基因的多态性成为目前研究的热点。维生素D受体基因是目前研究最多也是最有争议的基因。在复习大量相关文献的基础上,该文就维生素D受体基因多态性与骨质疏松症的关系予以综述。 展开更多
关键词 骨质疏松症 基因 多态性 维生素D受体基因
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Discovery of leaf region and time point related modules and genes in maize(Zea mays L.)leaves by Weighted Gene Co-expression Network analysis(WGCNA)of gene expression profiles of carbon metabolism 预览
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作者 WANG Jing-lu ZHANG Ying +3 位作者 PAN Xiao-di DU Jian-jun MA Li-ming GUO Xin-yu 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第2期350-360,共11页
Maize(Zea mays L.) yield depends not only on the conversion and accumulation of carbohydrates in kernels, but also on the supply of carbohydrates by leaves. However, the carbon metabolism process in leaves can vary ac... Maize(Zea mays L.) yield depends not only on the conversion and accumulation of carbohydrates in kernels, but also on the supply of carbohydrates by leaves. However, the carbon metabolism process in leaves can vary across different leaf regions and during the day and night. Hence, we used Weighted Gene Co-expression Network analysis(WGCNA) with the gene expression profiles of carbon metabolism to identify the modules and genes that may associate with particular regions in a leaf and time of day. There were a total of 45 samples of maize leaves that were taken from three different regions of a growing maize leaf at five time points. Robust Multi-array Average analysis was used to pre-process the raw data of GSE85963(accession number), and quality control of data was based on Pearson correlation coefficients. We obtained eight co-expression network modules. The modules with the highest significance of association with LeafRegion and TimePoint were selected. Functional enrichment and gene-gene interaction analyses were conducted to acquire the hub genes and pathways in these significant modules. These results can support the findings of similar studies by providing evidence of potential module genes and enriched pathways associated with leaf development in maize. 展开更多
关键词 WGCNA MAIZE leaf GENE expression GENE MODULES PATHWAYS
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Up-regulation of a homeodomain-leucine zipper gene HD-1 contributes to trichome initiation and development in cotton 预览
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作者 NIU Er-li CAI Cai-ping +3 位作者 BAO Jiang-hao WU Shuang ZHAO Liang GUO Wang-zhen 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第2期361-371,共11页
Plant trichomes originate from epidermal cells.In this work,we demonstrated that a homeodomain-leucine zipper(HD-Zip)gene,GhA06G1283(Gh HD-1A),was related to the leaf trichome trait in allotetraploid cotton and could ... Plant trichomes originate from epidermal cells.In this work,we demonstrated that a homeodomain-leucine zipper(HD-Zip)gene,GhA06G1283(Gh HD-1A),was related to the leaf trichome trait in allotetraploid cotton and could be a candidate gene for the T1 locus.The ortholog of GhHD-1A in the hairless accession Gossypium barbadense cv.Hai7124 was interrupted by a long terminal repeat(LTR)retrotransposon,while GhHD-1A worked well in the hairy accession Gossypium hirsutum acc.T586.Sequence and phylogenetic analysis showed that GhHD-1A belonged to the HD-Zip IV gene family,which mainly regulated epidermis hair development in plants.Silencing of GhHD-1A and its homoeologs GhHD-1D in allotetraploid T586and Hai7124 could significantly reduce the density of leaf hairs and affect the expression levels of other genes related to leaf trichome formation.Further analysis found that GhHD-1A mainly regulated trichome initiation on the upper epidermal hairs of leaves in cotton,while the up-regulated expression of GhHD-1A in different organs/tissues also altered epidermal trichome development.This study not only helps to unravel the important roles of GhHD-1A in regulating trichome initiation in cotton,but also provides a reference for exploring the different forms of trichome development in plants. 展开更多
关键词 leaf TRICHOME map-based cloning a homeodomain-leucine ZIPPER GENE HD-1 virus-induced GENE silencing (VIGS) functional differentiation
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钴基高温合金专利技术分析 预览
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作者 郭洁琼 杨夏琼 +1 位作者 田恩华 王慧萍 《河南科技》 2019年第21期53-55,共3页
本文以钴基高温合金专利技术为分析对象,重点分析国内外钴基高温合金专利技术的申请信息、早期专利、重要申请人以及技术发展情况,帮助国内相关企业更好地利用专利信息,提高国内钴基高温合金企业的竞争力。
关键词 钴基高温合金 GENE SIEI UNAC 专利分析
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关于基因编辑的伦理反思 预览
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作者 孙伟平 戴益斌 《重庆大学学报:社会科学版》 CSSCI 北大核心 2019年第4期1-9,共9页
基因编辑是一项新兴的复杂的前沿科学技术,它如果得到安全、合理的利用,无疑具有重要的积极效应;但关键是它目前并不成熟,在缺乏严格的科学评估、安全性存在不可预知风险的情况下,贸然使用可能导致人类基因谱系发生改变等问题,甚至带来... 基因编辑是一项新兴的复杂的前沿科学技术,它如果得到安全、合理的利用,无疑具有重要的积极效应;但关键是它目前并不成熟,在缺乏严格的科学评估、安全性存在不可预知风险的情况下,贸然使用可能导致人类基因谱系发生改变等问题,甚至带来难以预料的灾难性后果。即使今后基因编辑技术成熟了,如何合理地运用它,而不偏离正确的轨道,也需要更审慎地进行伦理反思,努力形成基本的伦理共识。为了促进基因编辑技术的健康发展,令其更好地兴利除弊,为人类造福,基因编辑技术的研究、应用必须遵循人本原则、公正原则、公开透明原则、知情同意原则、责任原则等基本的伦理原则。 展开更多
关键词 基因 基因编辑 伦理反思 伦理后果 伦理原则 科学伦理
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Study on gene expression patterns and functional pathways of peripheral blood monocytes reveals potential molecular mechanism of surgical treatment for periodontitis 预览
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作者 Jin-Ji Ma Hong-Mei Liu +2 位作者 Xiang-Hua Xu Li-Xin Guo Qing Lin 《世界临床病例杂志》 2019年第12期1383-1392,共10页
BACKGROUND Periodontitis is a chronic inflammation of periodontal supporting tissue caused by local factors. Periodontal surgery can change the gene expression of peripheral blood mononuclear cells. However, little is... BACKGROUND Periodontitis is a chronic inflammation of periodontal supporting tissue caused by local factors. Periodontal surgery can change the gene expression of peripheral blood mononuclear cells. However, little is known about the potential mechanism of surgical treatment for periodontitis. AIM To explore the potential molecular mechanism of surgical treatment for periodontitis. METHODS First, based on the expression profiles of genes related to surgical treatment for periodontitis, a set of expression disorder modules related to surgical treatment for periodontitis were obtained by enrichment analysis. Subsequently, based on crosstalk analysis, we proved that there was a significant crosstalk relationship between module 3 and module 5. Finally, based on predictive analysis of multidimensional regulators, we identified a series of regulatory factors, such as endogenous genes, non-coding RNAs (ncRNAs), and transcription factors, which have potential regulatory effects on periodontitis. RESULTS A total of 337 genes related to surgical treatment for periodontitis were obtained, and 3896 genes related to periodontitis were amplified. Eight expression modules of periodontitis were obtained, involving the aggregation of 2672 gene modules. These modules are mainly involved in G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger, and adenylate cyclasemodulating G-protein coupled receptor signaling pathway. In addition, eight endogenous genes (including EGF, RPS27A, and GNB3) were screened by network connectivity analysis. Finally, based on this set of potential dysfunction modules, 94 transcription factors (including NFKB1, SP1, and STAT3) and 1198 ncRNAs (including MALAT1, CRNDE, and ANCR) were revealed. These core regulators are thought to be involved in the potential molecular mechanism of periodontitis after surgical treatment. CONCLUSION Based on the results of this study, we can show biologists and pharmacists a new idea to reveal the potential molecular mechanism of surgic 展开更多
关键词 Peripheral blood MONONUCLEAR cells GENE EXPRESSION DYSREGULATION module POTENTIAL molecular mechanism GENE EXPRESSION pattern
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Genome-wide identification and expression analysis of auxin response factor(ARF) gene family in strawberry(Fragaria vesca) 预览
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作者 WANG Shao-xi SHI Feng-yan +3 位作者 DONG Xiang-xiang LI Yu-xiang ZHANG Zhi-hong LI He 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第7期1587-1603,共17页
Auxin signaling plays a significant role in the whole process of plant growth and development from embryogenesis to senescence.Auxin response factors(ARFs) are reported to regulate the expression of auxin response gen... Auxin signaling plays a significant role in the whole process of plant growth and development from embryogenesis to senescence.Auxin response factors(ARFs) are reported to regulate the expression of auxin response genes by binding to auxin response elements.ARF is the most critical transcription factor family which has been released in most species,but few reports in strawberry.In this study,the structure characterization of 12 FvARF genes in strawberry,their expression patterns at different development stages,different organizations,and different indole-3-acetic acid(IAA) treatments were analyzed.The expression of 12 FvARFs was found in all experiment tissues and showed almost the same trend during fruit development.All FvARFs respond to the treatment of IAA.Our study provides comprehensive information on ARF family in strawberry,including gene structures,chromosome locations,phylogenetic relationships and expression patterns.The information on FvARF genes paves the way for future research on strawberry ARF genes. 展开更多
关键词 AUXIN AUXIN response factor FRAGARIA vesca GENE structure GENE expression
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Phylogenetic and Expression Analyses of With-No-Lysine Kinase Genes Reveal Novel Gene Family Diversity in Fruit Trees
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作者 Suhao Cao Pingping Hao +4 位作者 Weishen Shu Guoming Wang Zhihua Xie Chao Gu Shaoling Zhang 《园艺学报:英文版》 2019年第2期47-58,共12页
With-No-Lysine kinases(WNK) have been reported to be associated with plant growth regulation in Arabidopsis, soybean, and rice, but little is known of their roles in fruits. In this study, a total of 114 WNK genes wer... With-No-Lysine kinases(WNK) have been reported to be associated with plant growth regulation in Arabidopsis, soybean, and rice, but little is known of their roles in fruits. In this study, a total of 114 WNK genes were identified from 8 fruit trees species, and these WNK genes belonged to 2 classes(I and II) that respectively contain 4(A, B, C, D) and 2 groups(E, F). The WNK genes had variable exon-intron structures and were randomly distributed among most chromosomes of each genus. The expression levels of six of the 18 WNK genes in peach were almost undetectable in fruits, suggesting that they may not be associated with fruit development and ripening. Of the other 12 WNK genes, PpWNK.B1 exhibited stronger stability of expression levels than the best reference genes reported previously in fruits from two peach cultivars. These results suggested that PpWNK.B1 could be a reliable reference gene for gene expression studies of peach fruit. Moreover, qRT-PCR assays yielded evidence that when using single reference gene as internal control, mistakes were more possible, indicating that the use of three reference genes is necessary for gene expression studies. Based on the gene expression profiles validated by qRT-PCR, PpWNK.A1 is predicted to likely be involved in fruit ripening, while PpWNK.A2 and PpWNK.E3.1 are likely associated with early fruit development. The present study represented the WNK genes in fruits, and it will be a valuable resource in continuing investigation of gene regulation network during fruit development and ripening. 展开更多
关键词 peach With-No-Lysine kinase PHYLOGENETIC tree reference GENE GENE EXPRESSION
Assessment of antibiotic resistance genes in dialysis water treatment processes
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作者 Xuan Zhu Chengsong Ye +2 位作者 Yuxin Wang Lihua Chen Lin Feng 《环境科学与工程前沿(英文)》 SCIE EI CAS CSCD 2019年第3期185-191,共7页
Dialysis water is directly related to the safety of hemodialysis patients, thus its quality is generally ensured by a stepwise water purification cascade. To study the effect of water treatment on the presence of anti... Dialysis water is directly related to the safety of hemodialysis patients, thus its quality is generally ensured by a stepwise water purification cascade. To study the effect of water treatment on the presence of antibiotic resistance genes (ARGs) in dialysis water, this study used propidium monoazide (PMA) in conjunction with high throughput quantitative PCR to analyze the diversity and abundance of ARGs found in viable bacteria from water having undergone various water treatment processes. The results indicated the presence of 35 ARGs in the eflfluents from the different water treatment steps. Twentynine ARGs were found in viable bacteria from the effluent following carbon filtration, the highest among all of the treatment processes, and at 6.96 Log (copies/L) the absolute abundance of the cphA gene was the highest. Two resistance genes, erm (36) and mtrD-02, which belong to the resistance categories macrolides-lincosamides-streptogramin B (MLSB) and other/efflux pump, respectively, were detected in the effluent following reverse osmosis treatment. Both of these genes have demonstrated the potential for horizontal gene transfer. These results indicated that the treated effluent from reverse osmosis, the final treatment step in dialysis-water production, was associated with potential health risks. 展开更多
关键词 DIALYSIS water Treatment process Antibiotic resistance GENE HIGH-THROUGHPUT QUANTITATIVE PCR HORIZONTAL GENE TRANSFER
Duplication and diversification of insulin genes in ray-finned fish
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作者 David M.Irwin 《动物学研究》 CAS CSCD 2019年第3期185-197,共13页
Insulin is a key hormone for the regulation of metabolism in vertebrates.Insulin is produced by pancreatic islet cells in response to elevated glucose levels and leads to the uptake of glucose by tissues such as liver... Insulin is a key hormone for the regulation of metabolism in vertebrates.Insulin is produced by pancreatic islet cells in response to elevated glucose levels and leads to the uptake of glucose by tissues such as liver and adipose tissue to store energy.Insulin also has additional functions in regulating development.Previous work has shown that the proglucagon gene,which encodes hormones counter regulating insulin,is duplicated in teleost fish,and that the peptide hormones encoded by these genes have diversified in function.I sought to determine whether similar processes have occurred to insulin genes in these species.Searches of fish genomes revealed an unexpected diversity of insulin genes.A triplication of the insulin gene occurred at the origin of teleost fish,however one of these three genes,insc,has been lost in most teleost fish lineages.The two other insulin genes,insa and insb,have been retained but show differing levels of selective constraint suggesting that they might have diversified in function.Intriguingly,a duplicate copy of the insa gene,which I named insab,is found in many fish.The coding sequenee encoded by insab genes is under weak selective constraint,with its predicted protein sequences losing their potential to be processed into a two-peptide hormone.However,these sequences have retained perfectly conserved cystine residues,suggesting that they maintain insulin's three-dimensional structure and therefore might modulate the processing and secretion of insulin produced by the other genes. 展开更多
关键词 INSULIN TELEOST fish GENE duplicati on Adaptive evolution GENE LOSS
Paddy Sys tern with a Hybrid Rice Enhances Cyanobac teria Nostoc and Increases N2 Fixation
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作者 MA Jing BEI Qicheng +6 位作者 WANG Xiaojie LIU Gang Georg CADISCH LIN Xingwu ZHU Jianguo SUN Xiaoli XIE Zubin 《土壤圈:英文版》 SCIE CAS CSCD 2019年第3期374-387,共14页
Biological nitrogen (N) fixation (BNF) plays a significant role in maintaining soil fertility in paddy field ecosystems. Rice variety influences BNF, but how different rice varieties regulate BNF and associated diazot... Biological nitrogen (N) fixation (BNF) plays a significant role in maintaining soil fertility in paddy field ecosystems. Rice variety influences BNF, but how different rice varieties regulate BNF and associated diazotroph communities has not been quantified. Airtight, field-based 15N2-labelling grow th chamber experiments were used to assess the BNF capac 辻 y of different rice varie ties. In addition, both the 16S rRNA and nifH genes were sequenced to assess the influence of different rice varieties on bacterial and diazotrophic communities in paddy soils. After subjecting a rice-soil system to 74 d of continuous airtight, field-based 15N2 labelling in pots in a growth chamber, the amounts of fixed N were 22.3 and 38.9 kg ha^-1 in inbred japonica (W23) and hybrid indica (IIY) rice cultivars plan ted in the rice-soil systems, respectively, and only 1%—2.5% of the fixed N was allocated to the rice plants and weeds. A greater abundance of diazotrophs was found in the surface soil (0-1 cm) under IIY than under W23. Sequencing of the 16S rRNA gene showed significantly greater abundances of the cyanobacterial genera Nostoc, Anabaena, and Cylindrospermum under IIY than under W23. Sequencing of the nifH gene also showed a significantly greater abundance of Nostoc under IIY than under W23. These results indicate that the hybrid rice cultivar (IIY) promoted BNF to a greater extent than the inbred rice cultivar (W23) and that the increase in BNF might have been due to the enhanced heterocystous cyanobacteria Nostoc. 展开更多
关键词 biological nitrogen FIXATION NIFH GENE 15N2 labelling rice variety 16S rRNA GENE
脊髓损伤后肌肉萎缩基因谱的生物信息学分析 预览
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作者 黄晖 王广积 《中国组织工程研究》 CAS 北大核心 2019年第27期4269-4274,共6页
背景:脊髓损伤后常伴随肌肉萎缩的发生,但是它的基本机制仍然不是十分清楚。目的:旨在探讨脊髓损伤后肌肉萎缩的分子生物学机制。方法:分析基因表达数据库中的脊髓损伤后肌肉萎缩的基因谱GSE45550。基因表达谱GSE45550包括对照组(脊髓... 背景:脊髓损伤后常伴随肌肉萎缩的发生,但是它的基本机制仍然不是十分清楚。目的:旨在探讨脊髓损伤后肌肉萎缩的分子生物学机制。方法:分析基因表达数据库中的脊髓损伤后肌肉萎缩的基因谱GSE45550。基因表达谱GSE45550包括对照组(脊髓损伤前)、实验组1(脊髓损伤后3 d)、实验组2(脊髓损伤后8 d)、实验组3(脊髓损伤后14 d)。组织为SD大鼠的比目鱼肌,每组6例。随后对4组样本数据进行差异基因分析、GO分析、通路分析。结果与结论:确定了2 513个差异表达基因,其中Wnt16、Obfc1、Ufd1l、LOC100361067、Hhatl、Fxyd1、Psmc4、Tasp1、Mettl21c、Ufd1l差异表达最显著。GO分析显示差异基因的主要生物学过程为biological_process、G蛋白偶联受体信号通路、对药物的反应、DNA依赖性转录、DNA依赖性转录的正调节、氧化还原过程、泛素依赖性蛋白分解代谢过程、凋亡过程、RNA聚合酶转录的正调控及脂肪酸β-氧化。信号通路如MAPK信号、细胞凋亡、柠檬酸循环(TCA循环)可能起到重要的作用。研究比较完整地揭示了脊髓损伤后肌肉萎缩基因谱的差异表达基因和所涉及的生物学过程和信号通路,其中Wnt16可能是脊髓损伤后肌肉萎缩中的关键基因,为未来的治疗进展提供分子靶点。 展开更多
关键词 脊髓损伤 肌肉萎缩 通路 基因 生物学过程 差异基因分析 GO分析 通路分析
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2545例新生儿遗传性耳聋基因突变筛查 预览
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作者 马宁 王艳 +2 位作者 彭薇 李昊 杨晓 《中国康复理论与实践》 CSCD 北大核心 2019年第4期444-447,共4页
目的了解中国人常见遗传性耳聋基因携带率和突变谱。方法采集2018年1月至10月本院出生的2545例新生儿足跟血,微阵列芯片法对常见耳聋基因突变进行筛查。结果共发现119例新生儿携带耳聋基因突变,其中GJB2突变携带者60例(2.36%)v男女比1∶... 目的了解中国人常见遗传性耳聋基因携带率和突变谱。方法采集2018年1月至10月本院出生的2545例新生儿足跟血,微阵列芯片法对常见耳聋基因突变进行筛查。结果共发现119例新生儿携带耳聋基因突变,其中GJB2突变携带者60例(2.36%)v男女比1∶1(30/30);SLC26A4突变携带者48例(1.88%),男女比接近1∶1(26/22);线粒体12SrRNA基因突变携带者5例(0.20%);GJB3突变携带者5例(0.20%);1例有GJB2基因235位点和SLC26A4基因IVS7-2位点双杂合突变(0.04%)。另外还发现SLC26A4基因1174A>T、1229C>T和15+5G>A突变各1例。结论初步了解中国人耳聋基因位点分布,可为遗传性耳聋防控参考。 展开更多
关键词 遗传性耳聋 新生儿 基因 突变 基因芯片 筛查
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191例感音神经性耳聋患者常见耳聋基因筛查结果分析 预览
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作者 芦婷 刘艳玲 +1 位作者 马琪琪 曹丽荣 《宁夏医学杂志》 CAS 2019年第2期155-157,共3页
目的分析宁夏地区191例感音神经性耳聋患者常见耳聋基因的突变情况,为临床防聋和治聋提供参考依据。方法对宁夏地区191例感音神经性耳聋患者采用晶芯15项遗传性耳聋基因检查的试剂进行4种常见耳聋基因15个突变位点检测。结果 191例受检... 目的分析宁夏地区191例感音神经性耳聋患者常见耳聋基因的突变情况,为临床防聋和治聋提供参考依据。方法对宁夏地区191例感音神经性耳聋患者采用晶芯15项遗传性耳聋基因检查的试剂进行4种常见耳聋基因15个突变位点检测。结果 191例受检者中检出耳聋基因突变90例,检出率为47. 12%,其中GJB2基因突变34例(17. 80%,34/191),SLC26A4基因突变45例(23. 56%,45/191),线粒体12rRNA基因突变2例(1. 05%,2/191),GJB2和SLC26A4基因突变8例(4. 19%,8/191),GJB3和SLC26A4基因突变1例(0. 52%,1/191),明确诊断为遗传性耳聋60例,提示耳聋基因携带者30例。结论宁夏地区感音神经性耳聋患者耳聋基因携带率较高,主要以SLC26A4基因c. 235delC位点突变为主,其次是GJB2基因IVS7-2A> G位点突变,对高危人群进行耳聋基因突变的筛查和遗传咨询是防止和控制遗传性耳聋、优生优育的重要手段。 展开更多
关键词 耳聋 基因 遗传 基因突变
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The CRIPSR/Cas gene-editing system—an immature but useful toolkit for experimental and clinical medicine 预览
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作者 Yuyan Yang Yue Huang 《动物模型与实验医学(英文)》 CSCD 2019年第1期5-8,共4页
A Chinese scientist,Jiankui He,and his creation of the world's first genetically altered baby made headlines recently.As a newly developed gene-editing technique,the CRISPR/Cas system should not be applied to huma... A Chinese scientist,Jiankui He,and his creation of the world's first genetically altered baby made headlines recently.As a newly developed gene-editing technique,the CRISPR/Cas system should not be applied to human beings for reproductive purposes until it has been extensively tested.However,numerous experimental research studies in human somatic,germline cells,and even in embryos,have been conducted,which have shown CRISPR/Cas to be a useful tool for human genome editing and a potential therapeutic method for future clinical use. 展开更多
关键词 CRISPR-Cas GENE EDITING GENE therapy human embryos RECOMBINANT DNA
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