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急性炎性疾病患者乳酸林格氏液液体动力学 预览
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作者 叶晓敏 易声华 +4 位作者 朱烨柯 单跃 腾尹彤 滕文彬 李玉红 《中国临床药理学与治疗学》 CAS CSCD 2019年第4期424-432,共9页
目的:探索炎性疾病患者的乳酸林格氏液(Ringer's lactate,RL)液体动力学特征以及炎性生物标记物是否可以作为协变量影响RL分布和排泄。方法:本研究为前瞻性队列研究。选择40例美国麻醉医师分级(ASA)Ⅰ-Ⅱ级,腹腔镜下择期胆囊切除术... 目的:探索炎性疾病患者的乳酸林格氏液(Ringer's lactate,RL)液体动力学特征以及炎性生物标记物是否可以作为协变量影响RL分布和排泄。方法:本研究为前瞻性队列研究。选择40例美国麻醉医师分级(ASA)Ⅰ-Ⅱ级,腹腔镜下择期胆囊切除术(胆囊炎组, n =20)或者腹腔镜下急诊阑尾切除术(阑尾炎组, n=20)。所有患者麻醉诱导前开始输注RL,按15 mL/kg,35 min内输毕。采用酶联免疫(enzyme-linked immunosorbent assay,ELISA)方法测定血浆炎症(TNF-α,IL-10和CRP)或者内皮损伤生物标记物(syndecan-1,SDC-1);利用血红蛋白(Hb)稀释-时间曲线和尿量,使用Phoenix软件,采用非线性混合效应模型分析计算RL液体动力学参数和协变量的影响。结果:与胆囊炎组相比,阑尾炎组RL从组织间隙到血浆的转运速率常数( k 21 )显著降低(14×10^-3 min^-1 versus 35×10^-3 min^-1;P = 0.012 )。阑尾炎组C反应蛋白(CRP)升高[中位数38.1( 1.8 - 143.6 )μg/mL versus 1.3( 0.1 - 159.0 )μg/mL;P < 0.001 ];与清醒状态相比,麻醉期间(输液开始后30~45 min),液体从中央室中到外周室的转运速率常数( k 12 )显著增加(57×10^-3 min^-1 versus 32×10^-3 min^-1;P < 0.01 )。清除速率常数( k 10 )降低90%( 0.6 ×10 -3 min^-1 versus 5.3 ×10^-3 min^-1;P < 0.001 )。无论在清醒状态还是麻醉状态下低血压均能降低液体清除;炎症或者内膜损伤的生物标记物不能作为显著影响RL液体动力学参数的协变量。结论:阑尾炎或者胆囊炎患者术前输入液体后“炎症反应的生物标记物”不是RL的液体动力学的协变量,但是两组患者中,全身麻醉期间输入液体的清除率下降。 展开更多
关键词 晶体液 乳酸林格氏液 人类 炎症 液体动力学
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Epidemiology of hepatitis E in South-East Europe in the"One Health"concept 预览
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作者 Anna Mrzljak Petra Dinjar-Kujundzic +5 位作者 Lorena Jemersic Jelena Prpic Ljubo Barbic Vladimir Savic Vladimir Stevanovic Tatjana Vilibic-Cavlek 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第25期3168-3182,共15页
The significance of hepatitis E virus(HEV)as an important public health problem is rising.Until a decade ago,cases of HEV infection in Eur-ope were mainly confined to returning travelers,but nowadays,hepatitis E repre... The significance of hepatitis E virus(HEV)as an important public health problem is rising.Until a decade ago,cases of HEV infection in Eur-ope were mainly confined to returning travelers,but nowadays,hepatitis E represents an emerging zoonotic infection in many European countries.The aim of this manuscript is to perform a systematic review of the published literature on hepatitis E distribution in humans,animals and environmental samples("One Health"concept)in the South-Eastern European countries.Comparison of the available data showed that the anti-HEV seroprevalence in the South-Eastern Europe varies greatly,depending on the population studied,geographical area and methods used.The IgG seroprevalence rates in different population groups were found to be 1.1%-24.5%in Croatia,up to 20.9%in Bulgaria,5.9-%17.1%in Romania,15%in Serbia,up to 9.7%in Greece and 2%-9.7%in Albania.Among possible risk factors,older age was the most significant predictor for HEV seropositivity in most studies.Higher seroprevalence rates were found in animals.HEV IgG antibodies in domestic pigs were detected in 20%-54.5%,29.2%-50%,38.94%-50%and 31.1%-91.7%in Serbia,Bulgaria,Romania and Croatia,respectively.In wild boars seroprevalence rates were up to 10.3%,30.3%and 31.1%in Romania,Slovenia and Croatia,respectively.A high HEV RNA prevalence in wild boars in some countries(Croatia and Romania)indicated that wild boars may have a key role in the HEV epidemiology.There are very few data on HEV prevalence in environmental samples.HEV RNA was detected in 3.3%and 16.7%surface waters in Slovenia and Serbia,respectively.There is no evidence of HEV RNA in sewage systems in this region.The available data on genetic characterization show that human,animal and environmental HEV strains mainly belong to the genotype 3. 展开更多
关键词 Hepatitis E virus "One-Health" Humans Animals Environment South-East EUROPE
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MicroRNAs as biomarkers of diabetic retinopathy and disease progression 预览
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作者 Bridget Martinez Philip V. Peplow 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1858-1869,共12页
Diabetes mellitus, together with its complications, has been increasing in prevalence worldwide. Its complications include cardiovascular disease(e.g., myocardial infarction, stroke), neuropathy, nephropathy, and eye ... Diabetes mellitus, together with its complications, has been increasing in prevalence worldwide. Its complications include cardiovascular disease(e.g., myocardial infarction, stroke), neuropathy, nephropathy, and eye complications(e.g., glaucoma, cataracts, retinopathy, and macular edema). In patients with either type 1 or type 2 diabetes mellitus, diabetic retinopathy is the leading cause of visual impairment or blindness. It is characterized by progressive changes in the retinal microvasculature. The progression from nonproliferative diabetic retinopathy to a more advanced stage of moderate to severe nonproliferative diabetic retinopathy and proliferative diabetic retinopathy occurs very quickly after diagnosis of mild nonproliferative diabetic retinopathy. The etiology of diabetic retinopathy is unclear, and present treatments have limited effectiveness. Currently diabetic retinopathy can only be diagnosed by a trained specialist, which reduces the population that can be examined. A screening biomarker of diabetic retinopathy with high sensitivity and specificity would aid considerably in identifying those individuals in need of clinical assessment and treatment. The majority of the studies reviewed identified specific microRNAs in blood serum/plasma able to distinguish diabetic patients with retinopathy from those without retinopathy and for the progresion of the disease from nonproliferative diabetic retinopathy to proliferative diabetic retinopathy. In addition,certain microRNAs in vitreous humor were dysregulated in proliferative diabetic retinopathy compared to controls. A very high percentage of patients with diabetic retinopathy develop Alzheimer’s disease. Thus, identifying diabetic retinopathy by measurement of suitable biomarkers would also enable better screening and treatment of those individuals at risk of Alzheimer’s disease. 展开更多
关键词 DIABETES RETINOPATHY diagnosis disease PROGRESSION MICRORNAS biomarkers blood serum/ plasma VITREOUS HUMOR humans
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MicroRNAs as diagnostic and therapeutic tools for Alzheimer's disease: advances and limitations 预览
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作者 Bridget Martinez Philip V. Peplow 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第2期242-255,共14页
Alzheimer's disease (AD) is the most common age-related, progressive neurodegenerative disease. It is characterized by memory loss and cognitive decline and responsible for most cases of dementia in the elderly. L... Alzheimer's disease (AD) is the most common age-related, progressive neurodegenerative disease. It is characterized by memory loss and cognitive decline and responsible for most cases of dementia in the elderly. Late-onset or sporadic AD accounts for > 95% of cases, with age at onset > 65 years. Currently there are no drugs or other therapeutic agents available to prevent or delay the progression of AD. The cellular and molecular changes occurring in the brains of individuals with AD include accumulation of β-amyloid peptide and hyperphosphorylated tau protein, decrease of acetylcholine neurotransmitter, inflammation, and oxidative stress. Aggregation of β-amyloid peptide in extracellular plaques and the hyperphosphorylated tau protein in intracellular neurofibrillary tangles are characteristic of AD. A major challenge is identifying molecular biomarkers of the early-stage AD in patients as most studies have been performed with blood or brain tissue samples (postmortem) at late-stage AD. Subjects with mild cognitive impairment almost always have the neuropathologic features of AD with about 50% of mild cognitive impairment patients progressing to AD. They could provide important information about AD pathomechanism and potentially also highlight minimally or noninvasive, easy-to-access biomarkers. MicroRNAs are dysregulated in AD, and may facilitate the early detection of the disease and potentially the continual monitoring of disease progression and allow therapeutic interventions to be evaluated. Four recent reviews have been published of microRNAs in AD, each of which identified areas of weakness or limitations in the reported studies. Importantly, studies in the last three years have shown considerable progress in overcoming some of these limitations and identifying specific microRNAs as biomarkers for AD and mild cognitive impairment. Further large-scale human studies are warranted with less disparity in the study populations, and using an appropriate method to validate the findings. 展开更多
关键词 Alzheimer's disease MILD cognitive IMPAIRMENT MICRORNAS biomarkers blood brain tissue CEREBROSPINAL fluid humans animal models
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RAINBOW WEAVERS
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作者 Yuan Yuan 《北京周报:英文版》 2019年第20期16-17,共2页
In the language of the Derung ethnic group in China, Naze Naze Brao means "weaving slowly." This tradition and technique has been handed down for generations by the women of the ethnic group who weave blanke... In the language of the Derung ethnic group in China, Naze Naze Brao means "weaving slowly." This tradition and technique has been handed down for generations by the women of the ethnic group who weave blankets on looms known to be the earliest ones used by humans. 展开更多
关键词 Derung ETHNIC GROUP WOMEN of the ETHNIC GROUP ONES USED by humans
《毕司沃斯先生的房子》的生态解读 预览
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作者 张奇才 王婷婷 《哈尔滨学院学报》 2018年第1期109-111,共3页
文章从生态批评角度解读奈保尔的代表作《毕司沃斯先生的房子》,指出作品中反映的第三世界前殖民地国家生态破坏的具体表现:人类的各种产业活动侵犯、污染了自然的领地,影响了自然界中动植物的生存,破坏了自然的和谐与美感;生态系... 文章从生态批评角度解读奈保尔的代表作《毕司沃斯先生的房子》,指出作品中反映的第三世界前殖民地国家生态破坏的具体表现:人类的各种产业活动侵犯、污染了自然的领地,影响了自然界中动植物的生存,破坏了自然的和谐与美感;生态系统中物种之一的人类在肮脏、破败的环境中孤立无助地遭受着精神和肉体的病痛。 展开更多
关键词 生态批评 人类 自然 审美
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Attaining Human Aspects to Avoid Alienation in Architecture 预览
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作者 Saqer Mustafa Sqour 《土木工程与建筑:英文版》 2018年第2期121-131,共11页
Architectural practice during the last century was influenced by Western architectural theories.These theories were reflections of the contradictions of human beings’lives:the result was contradictions in architectur... Architectural practice during the last century was influenced by Western architectural theories.These theories were reflections of the contradictions of human beings’lives:the result was contradictions in architecture.The research aims to launch a view of the fundamentals of what we might call Muslim architectural theory,fundamentals that can control the future of architecture.Thus,it offers a set of principles coming from Muslim thought;these principles highlight humanitarian needs to be fulfilled by architecture.This paper makes the following assumptions:(1)Re-forming architecture requires re-forming the human.(2)The interaction between the social environment on one side and the built environment on the other side achieves human comfort.(3)Principles derived from Muslim thought set up general rules that can order architectural practice.Based on these theories,this paper has a goal of crystallizing a new theory that governs present-day architecture.Thus,the paper studies human needs in architecture.Besides,it has an implementation part to explain how architecture fulfills human needs.It further shows the extent to which the built environment can satisfy those needs.The research includes thirty needs traced from Muslim thought.Consequently,the research examined the presence of these needs in one traditional Arabian house. 展开更多
关键词 HUMANITY in ARCHITECTURE ALIENATION in ARCHITECTURE HUMAN ASPECTS in ARCHITECTURE ARCHITECTURE for humans
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Diagnosis and management of fibromuscular dysplasia and segmental arterial mediolysis in gastroenterology field:A mini-review 预览
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作者 Masayoshi Ko Kenya Kamimura +6 位作者 Kohei Ogawa Kentaro Tominaga Akira Sakamaki Hiroteru Kamimura Satoshi Abe Kenichi Mizuno Shuji Terai 《世界胃肠病学杂志:英文版》 SCIE CAS 2018年第32期3637-3649,共13页
The vascular diseases including aneurysm,occlusion,and thromboses in the mesenteric lesions could cause severe symptoms and appropriate diagnosis and treatment are essential for managing patients.With the development ... The vascular diseases including aneurysm,occlusion,and thromboses in the mesenteric lesions could cause severe symptoms and appropriate diagnosis and treatment are essential for managing patients.With the development and improvement of imaging modalities,diagnostic frequency of these vascular diseases in abdominal lesions is increasing even with the small changes in the vasculatures.Among various vascular diseases,fibromuscular dysplasia(FMD)and segmental arterial mediolysis(SAM)are noninflammatory,nonatherosclerotic arterial diseases which need to be diagnosed urgently because these diseases could affect various organs and be lethal if the appropriate management is not provided.However,because FMD and SAM are rare,the cause,prevalence,clinical characteristics including the symptoms,findings in the imaging studies,pathological findings,management,and prognoses have not been systematically summarized.Therefore,there have been neither standard diagnostic criteria nor therapeutic methodologies established,to date.To systematically summarize the information and to compare these disease entities,we have summarized the characteristics of FMD and SAM in the gastroenterological regions by reviewing the cases reported thus far.The information summarized will be helpful for physicians treating these patients in an emergency care unit and for the differential diagnosis of other diseases showing severe abdominal pain. 展开更多
关键词 Fibromuscular DYSPLASIA SEGMENTAL arterial mediolysis MESENTERIC LESION diagnosis Humans
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紫外线照射诱导的人晶状体上皮细胞中谷氧还蛋白2上调对细胞凋亡的抑制作用 预览 被引量:2
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作者 郭辰峻 郭勇 +2 位作者 王嵩 张婕 严宏 《中华实验眼科杂志》 CSCD 北大核心 2018年第3期169-175,共7页
目的观察不同能量紫外线(UV)B照射对人晶状体上皮细胞(LECs)的损伤作用及细胞线粒体中谷氧还蛋白2(Grx2)的表达变化,探讨Grx2对UVB诱导的人LECs凋亡的抑制作用。方法对HLE-B3进行体外培养,以不同能量的UVB(0、10、30、50 mJ/cm... 目的观察不同能量紫外线(UV)B照射对人晶状体上皮细胞(LECs)的损伤作用及细胞线粒体中谷氧还蛋白2(Grx2)的表达变化,探讨Grx2对UVB诱导的人LECs凋亡的抑制作用。方法对HLE-B3进行体外培养,以不同能量的UVB(0、10、30、50 mJ/cm2)(波长297 nm)分别照射培养的细胞,分别于照射后2、4、8、12和16 h在光学显微镜下观察细胞形态变化;采用细胞计数试剂盒8(CCK8)法检测各组细胞的存活率;采用TUNEL法测定各组细胞的凋亡率;分别采用实时荧光定量PCR和Western blot法检测各组细胞中Grx2 mRNA及其蛋白的相对表达量。用pcDNA3.1-Grx2质粒转染培养的细胞构建Grx2过表达模型,以pcDNA3.1空质粒转染组作为对照,并以UVB照射转染的细胞,采用TUNEL法检测各组细胞的凋亡率。结果细胞培养过程中未照射组细胞贴壁生长,细胞伸展良好,细胞中Grx2表达呈绿色荧光。UVB照射的细胞皱缩变小,死亡细胞增多,10、30、50 mJ/cm2 UVB照射后随着UVB剂量增加和照射时间延长细胞存活率逐渐下降,凋亡细胞逐渐增多。10 mJ/cm2 UVB照射组和30 mJ/cm2 UVB照射组照射后4 h,细胞中Grx2 mRNA相对表达量分别为2.53±0.48和3.53±0.14,均明显高于未照射组的1.01±0.08和1.00±0.09,50 mJ/cm2 UVB照射组照射后1 h细胞中Grx2 mRNA相对表达量为15.30±3.01,明显高于未照射组的1.00±0.07,差异均有统计学意义(均P〈0.05);各照射不同时间后细胞中Grx2蛋白相对表达量变化趋势与mRNA相同。50 mJ/cm2 UVB照射后4 h Grx2转染组细胞凋亡率为(15.34±1.71)%,明显低于空质粒组的(22.11±2.46)%,差异有统计学意义(t=3.189,P〈0.05)。结论不同能量UVB照射后诱导的人LECs凋亡和损伤程度呈UVB能量依赖性和照射时间依赖性,各剂量UVB照射LECs后细胞中Grx2表达量均表现为一过性上调,Grx2表达量增加对UVB诱导人LECs凋亡有抑制作用 展开更多
关键词 紫外线/不良反应 上皮细胞 晶状体 谷氧还蛋白 凋亡/辐射作用 培养细胞
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载脂蛋白M对高糖诱导的人视网膜血管内皮细胞中相关炎性因子表达的抑制作用 预览 被引量:1
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作者 唐皖 罗光华 +5 位作者 姚霜 王敏 潘丽莉 喻妙梅 于洋 刘瑶 《中华实验眼科杂志》 CSCD 北大核心 2018年第3期194-198,共5页
目的观察高糖培养环境下人视网膜血管内皮细胞(HRECs)中载脂蛋白M(ApoM)、肿瘤坏死因子-α(TNF-α)和单核细胞趋化因子-1(MCP-1)表达的变化,探讨ApoM过表达对高糖诱导的HRECs中TNF-α和MCP-1表达的抑制作用。方法采用含体积分... 目的观察高糖培养环境下人视网膜血管内皮细胞(HRECs)中载脂蛋白M(ApoM)、肿瘤坏死因子-α(TNF-α)和单核细胞趋化因子-1(MCP-1)表达的变化,探讨ApoM过表达对高糖诱导的HRECs中TNF-α和MCP-1表达的抑制作用。方法采用含体积分数10%胎牛血清(FBS)和5.5 mmol/L D-葡萄糖的DMEM培养基培养HRECs后分为6个组。正常对照组细胞进行常规培养,高糖组细胞用含30 mmol/L D-葡萄糖的高糖培养基进行培养,ApoM过表达组用载有ApoM序列的慢病毒载体感染常规培养的细胞,空载组用无ApoM序列的慢病毒载体感染常规培养的细胞,空载+高糖组用高糖培养基培养空载体感染的细胞,ApoM过表达+高糖组用高糖培养基培养ApoM感染的细胞。采用实时荧光定量PCR法检测细胞中ApoM、TNF-α和MCP-1 mRNA相对表达量;采用Western blot法检测细胞中ApoM蛋白相对表达量。结果实时荧光定量PCR法检测显示,高糖组细胞中ApoM、TNF-α和MCP-1 mRNA相对表达量明显高于正常对照组,差异均有统计学意义(t=5.517、3.295、2.555,均P〈0.05)。HRECs感染慢病毒后生长良好,ApoM过表达组细胞中ApoM mRNA相对表达量为236.400±39.270,明显高于空载组的1.000±0.153,差异有统计学意义(t=5.995,P〈0.01),空载组细胞中未见ApoM蛋白表达条带,ApoM过表达组蛋白表达条带较强。正常培养基和高糖培养基培养后24 h,ApoM过表达组中ApoM蛋白相对表达量分别为1.000±0.249和2.978±0.285,差异有统计学意义(t=5.056,P〈0.01)。空载组、空载+高糖组、ApoM过表达组、ApoM过表达+高糖组细胞中TNF-α和MCP-1 mRNA相对表达量的总体比较差异均有统计学意义(F=5.966,P=0.026;F=14.410,P=0.002),ApoM过表达+高糖组细胞中TNF-α mRNA和MCP-1 mRNA相对表达量明显低于空载+高糖组,差异均有统计学意义(P=0.017、0.004)。 结论高糖培养� 展开更多
关键词 载脂蛋白M 葡萄糖/药物作用 炎症反应 视网膜 血管内皮细胞 单核细胞趋化 因子-1 肿瘤坏死因子-α
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Avian preference for close proximity to human habitation and its ecological consequences
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作者 Anders Pape MOLLER Mario DiAZ 《动物学报:英文版》 SCIE CAS CSCD 2018年第5期623-630,共8页
关键词 居住 偏爱 生态 繁殖季节 野生动物 人口密度 种系发生
肝细胞生长因子对转化生长因子-β1诱导的人Tenon囊成纤维细胞增生及转分化的抑制作用 预览
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作者 陈静 王泳 李东豪 《中华实验眼科杂志》 CSCD 北大核心 2018年第12期925-930,共6页
目的 研究肝细胞生长因子(HGF)对转化生长因子.β1(TGF.β1)诱导的人Tenon囊成纤维细胞增生和转分化的影响.方法 人Tenon囊成纤维细胞进行常规培养后分为空白对照组、TGF.β1处理组及不同质量浓度HGF+TGF.β1组.TGF.β1处理组在细胞培... 目的 研究肝细胞生长因子(HGF)对转化生长因子.β1(TGF.β1)诱导的人Tenon囊成纤维细胞增生和转分化的影响.方法 人Tenon囊成纤维细胞进行常规培养后分为空白对照组、TGF.β1处理组及不同质量浓度HGF+TGF.β1组.TGF.β1处理组在细胞培养液中添加10μg/L TGF.β1,不同质量浓度HGF+TGF.β1组在细胞培养液中分别添加10μg/L TGF.β1,然后分别添加不同质量浓度的HGF(25、50、100、200μg/L),采用甲基偶氮四唑(MTT)法检测波长560 nm处各组细胞的吸光度(A560);然后选用100μg/L的HGF进行干预,采用细胞免疫荧光染色技术检测人Tenon囊成纤维细胞中α.平滑肌肌动蛋白(α.SMA)的表达分布;采用Western blot法检测细胞中α.SMA蛋白的相对表达量.结果 体外培养的人Tenon囊成纤维细胞呈长梭形,边界清楚,细胞中波形蛋白表达阳性并定位于细胞质.MTT检测显示空白对照组、TGF.β1处理组及HGF25μg/L+TGF.β1组、HGF50μg/L+TGF.β1组、HGF100μg/L+TGF.β1组、HGF200μg/L+TGF.β1组细胞的增生值分别为0.203±0.025、0.497±0.101、0.426±0.062、0.354±0.040、0.272±0.084和0.241±0.011,组间比较差异有统计学意义(F=9.210,P=0.003),TGF.β1处理组细胞增生值明显高于空白对照组,不同质量浓度HGF+TGF.β1组细胞增生值均明显低于TGF.β1处理组,差异均有统计学意义(均P<0.05).免疫荧光染色结果显示空白对照组细胞中未见α.SMA的表达,TGF.β1处理组及HGF100μg/L+TGF.β1组细胞的胞质中均可见α.SMA的表达,呈红色荧光,HGF100μg/L+TGF.β1组细胞中α.SMA表达荧光减弱,α.SMA表达细胞明显减少.TGF.β1处理组和HGF100μg/L+TGF.β1组细胞中α.SMA染色阳性细胞百分比分别为(60.0±4.7)%和(14.3±3.1)%,差异有统计学意义(t=19.856,P<0.001).Western blot检测显示空白对照组、TGF.β1处理组和HGF100μg/L+TGF.β1组细胞中α.SMA蛋白相对表达量分别为0.642±0.032、1.330±0.069和0.884±0.040,总体比较差异有统计学意义(F=13.370 展开更多
关键词 Tenon囊/细胞学 成纤维细胞/代谢 肝细胞生长因子/应用及剂量 转化生长因子β1 纤维化/预防和控制 Α-平滑肌肌动蛋白 培养细胞
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Kuippel样因子6经活化转录因子4通路对晶状体上皮细胞凋亡的调控作用 预览
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作者 田芳 赵今稚 +8 位作者 滕贺 黄亮瑜 刘勋 苏睿虹 高美子 张晓敏 李筱荣 东莉洁 张红 《中华实验眼科杂志》 CSCD 北大核心 2018年第3期181-186,共6页
目的以活化转录因子4(ATF4)为靶点,探讨Krüppel样因子6(KLF6)调控紫外线B(UVB)诱导的晶状体上皮细胞(HLECs)凋亡的分子机制。方法HLECs系(HLE-B3)进行常规培养,采用脂质体转染法将构建的真核表达质粒pEGFP-C2-ATF4转染... 目的以活化转录因子4(ATF4)为靶点,探讨Krüppel样因子6(KLF6)调控紫外线B(UVB)诱导的晶状体上皮细胞(HLECs)凋亡的分子机制。方法HLECs系(HLE-B3)进行常规培养,采用脂质体转染法将构建的真核表达质粒pEGFP-C2-ATF4转染HLECs作为UVB+ATF4转染组,并用能量密度为20 mJ/cm2的UVB照射细胞;未转染的ATF4细胞作为正常对照组。采用苏木精-伊红染色法和Hoechst染色法观察ATF4对HLECs细胞形态学的影响。将培养的细胞分为KLF6转染组及相应空质粒对照组,小干扰KLF6(siKLF6)组(转染pSilencer-KLF6质粒)及相应的空载体对照组(转染pSilencer空质粒),采用Western blot法检测细胞中ATF4蛋白的相对表达量。将培养的细胞分为4个组,联合空载体组HLECs联合转染pEGFP-C2空载体和pSilencer空载体;KLF6+pSilencer空质粒组HLECs联合转染pEGFP-C2-KLF6和pSilencer空载体;小干扰ATF4(siATF4)+pEGFP-C2组HLECs联合转染pEGFP-C2空载体和pSilencer-ATF4;KLF6+siATF4组HLECs联合转染pEGFP-C2-KLF6和pSilencer-ATF4,各组细胞均暴露于UVB 200 s,采用ELISA法检测上述各组HLECs凋亡值。结果正常对照组培养的HLECs大小均匀,排列整齐,细胞核呈卵圆形,数量多且完整;UVB照射后部分细胞核固缩,细胞间隙增大,少数细胞出现核分裂;UCB+ATF4转染组细胞数量减少,多数细胞出现核固缩和核分裂。UVB+ATF4转染组细胞中ATF4蛋白表达条带灰度明显强于UVB+空载体组,ATF4蛋白相对表达量分别为0.99±0.06和0.13±0.02,差异有统计学意义(t=23.13,P〈0.01)。KLF6转染组细胞中KLF6和ATF4蛋白相对表达量均明显高于其空载体对照组,siKLF6组细胞中KLF6和ATF4蛋白相对表达量均明显低于其空载体对照组,差异均有统计学意义(均P〈0.01)。ELISA检测显示ATF4转染组HLECs凋亡值为1.37±0.11,明显高于正常对照组的0.31±0.11,差异有统计学意 展开更多
关键词 Kuippel样因子6 Kruppel样转录因子/代谢 活化转录因子4 晶状体上皮细胞 晶状体 细胞株 凋亡
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Mercury levels in human population from a mining district in Western Colombia
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作者 Harry Gutiérrez-Mosquera S.B.Sujitha +5 位作者 M.P.Jonathan S.K.Sarkar Fairy Medina-Mosquera Helcias Ayala-Mosquera Gladis Morales-Mira Laura Arreola-Mendoza 《环境科学学报:英文版》 SCIE EI CAS CSCD 2018年第6期83-90,共8页
关键词 哥伦比亚 采矿区 水银 人口 西方 THG 几何平均数 生物样品
重组人血小板源性生长因子对人视网膜血管内皮细胞生物学行为的促进作用及其机制 预览
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作者 李丹 刘高勤 +2 位作者 陈磊 王梦娇 陆培荣 《中华实验眼科杂志》 CSCD 北大核心 2018年第1期34-39,共6页
目的探讨重组人血小板源性生长因子-BB(rhPDGF-BB)对人视网膜血管内皮细胞(hRVECs)增生和迁移的影响及其作用机制。方法采用含体积分数10%胎牛血清的DMEM培养液培养hRVECs,分别将10、50和200 ng/ml rhPDGF-BB加入对数生长期hRVECs... 目的探讨重组人血小板源性生长因子-BB(rhPDGF-BB)对人视网膜血管内皮细胞(hRVECs)增生和迁移的影响及其作用机制。方法采用含体积分数10%胎牛血清的DMEM培养液培养hRVECs,分别将10、50和200 ng/ml rhPDGF-BB加入对数生长期hRVECs的培养液,未添加rhPDGF-BB者作为正常对照组。采用细胞计数试剂盒-8(CCK8)法检测各组细胞的增生情况;采用细胞划痕法检测细胞相对迁移面积(迁移后无细胞区面积/划痕初期无细胞区面积);采用逆转录PCR法检测hRVECs中rhPDGF-BB受体(rhPDGF-BBR)mRNA的相对表达量;采用实时荧光定量PCR法检测hRVECs中VEGF mRNA和整合素mRNA相对表达量。结果培养的hRVECs生长良好,用rhPDGF-BBR引物能扩增出与引物设计长度相符的表达条带。正常对照组及10、50和200 ng/ml rhPDGF-BB组培养细胞后24 h细胞增生值(A)分别为1.01±0.05、1.09±0.04、1.10±0.02和1.13±0.05,10、50和200 ng/ml rhPDGF-BB组A值明显高于正常对照组,差异均有统计学意义(t=2.504、3.430、3.483,均P〈0.05);细胞划痕试验后24 h,正常对照组及10、50和200 ng/ml rhPDGF-BB组细胞相对迁移面积分别为0.42±0.10、0.38±0.09、0.55±0.06和0.61±0.05,划痕试验后48 h细胞相对迁移面积分别为0.75±0.06、0.81±0.02、0.87±0.02和0.98±0.02,总体比较差异均有统计学意义(F分组=16.283,P=0.000;F时间=209.129,P=0.000),随着rhPDGF-BB剂量增加和作用时间延长,细胞相对迁移面积均明显增加;实时荧光定量PCR法检测显示正常对照组及10、50和200 ng/ml rhPDGF-BB组hRVECs中整合素mRNA相对表达量分别为1.06±0.02、1.30±0.10、1.20±0.16和1.27±0.08,VEGF mRNA相对表达量分别为0.97±0.05、1.06±0.16、1.58±0.18和1.66±0.21,其中50 ng/ml和200 ng/ml rhPDGF-BB组细胞中整合素mRNA及VEGF mRNA相对表达量均明显高于正常对照组,差异均有统计学意义(整合素mRNA:t=3.900 展开更多
关键词 血管内皮细胞/细胞学 病理性新生血管/代谢 血小板源性生长因子 血小板源性生长因子受体 重组蛋白 细胞培养 整合素 血管内皮生长因子
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神话与传说系列——南非 预览
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作者 英国大使馆文化教育处 《疯狂英语:初中版》 2018年第7期61-62,共2页
People from South Africa say that the Impundulu bird is as big as a person.Its legs and beak are red like fire.It moves its wings to make the noise you hear in a storm.You will also see lightning touch the ground when... People from South Africa say that the Impundulu bird is as big as a person.Its legs and beak are red like fire.It moves its wings to make the noise you hear in a storm.You will also see lightning touch the ground when it flies down and leaves an egg.People say that it is always thirsty for blood,which is why it kills humans and cows.People believe this strange bird can live forever,which means it never dies.It is very difficult to kill an Impundulu,because it can only be destroyed by fire. 展开更多
关键词 Impundulu kills humans FIRE
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全膝关节置换术中运动学轴线对位的概念与运用 预览
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作者 李珏宏 李亚民 +1 位作者 彭晓春 陈云苏 《中华关节外科杂志(电子版)》 CAS 2018年第6期796-801,共6页
全膝关节置换术后难以提高的患者满意度制约着手术效果的进一步提高。近年来运动轴线对位概念的提出引起了学者的关注。这种对位方式还原了膝关节的正常运动学轴线,因而有望进一步改善患者的功能恢复及生理感受。本文将从运动学轴线对... 全膝关节置换术后难以提高的患者满意度制约着手术效果的进一步提高。近年来运动轴线对位概念的提出引起了学者的关注。这种对位方式还原了膝关节的正常运动学轴线,因而有望进一步改善患者的功能恢复及生理感受。本文将从运动学轴线对位产生的理论依据,运动轴线对位的定义及手术原则,研究现状及目前关注的焦点几个方面作一综述,以期加深对这一概念的认识。 展开更多
关键词 关节成形术 置换 人类 膝关节 解剖学
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Functional magnetic resonance imaging and the brain: A brief review 预览
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作者 Maggie SM Chow Sharon L Wu +2 位作者 Sarah E Webb Katie Gluskin DT Yew 《世界放射学杂志:英文版(电子版)》 2017年第1期5-9,共5页
Functional magnetic resonance imaging(fMRI) is em-ployed in many behavior analysis studies, with blood oxygen level dependent-(BOLD-) contrast imaging being the main method used to generate images. The use of BOLD-con... Functional magnetic resonance imaging(fMRI) is em-ployed in many behavior analysis studies, with blood oxygen level dependent-(BOLD-) contrast imaging being the main method used to generate images. The use of BOLD-contrast imaging in f MRI has been refined over the years, for example, the inclusion of a spin echo pulse and increased magnetic strength were shown to produce better recorded images. Taking careful precautions to control variables during measurement, comparisons between different specimen groups can be illustrated by f MRI imaging using both quantitative and qualitative methods. Differences have been observed in comparisons of active and resting, developing and aging, and defective and damaged brains in various studies. However, cognitive studies using f MRI still face a number of challenges in interpretation that can only be overcome by imaging large numbers of samples. Furthermore, f MRI studies of brain cancer, lesions and other brain pathologies of both humans and animals are still to be explored. 展开更多
关键词 功能的磁性的回声图象 血氧水平依赖者成像 猪和啮齿类动物模型 老化 药效果 大脑损害和疾病
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Generalizable items and modular structure for computerised physician staffing calculation on intensive care units 预览
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作者 Manfred Weiss Gernot Marx Thomas Iber 《世界重症医学杂志》 2017年第3期153-163,共11页
Intensive care medicine remains one of the most costdriving areas within hospitals with high personnel costs.Under the scope of limited budgets and reimbursement,realistic needs are essential to justify personnel staf... Intensive care medicine remains one of the most costdriving areas within hospitals with high personnel costs.Under the scope of limited budgets and reimbursement,realistic needs are essential to justify personnel staffing.Unfortunately,all existing staffing models are top-down calculations with a high variability in results.We present a workload-oriented model,integrating quality of care,efficiency of processes,legal,educational,controlling,local,organisational and economic aspects.In our model,the physician’s workload solely related to the intensive care unit depends on three tasks:Patient-oriented tasks,divided in basic tasks(performed in every patient)and additional tasks(necessary in patients with specific diagnostic and therapeutic requirements depending on their specific illness,only),and non patient-oriented tasks.All three tasks have to be taken into account for calculating the required number of physicians.The calculation tool further allows to determine minimal personnel staffing,distribution of calculated personnel demand regarding type of employee due to working hours per year,shift work or standby duty.This model was introduced and described first by the German Board of Anesthesiologists and the German Society of Anesthesiology and Intensive Care Medicine in 2008 and since has been implemented and updated 2012 in Germany.The modular,flexible nature of the Excel-based calculation tool should allow adaption to the respective legal and organizational demands of different countries.After 8 years of experience with this calculation,we report the generalizable key aspects which may help physicians all around the world to justify realistic workload-oriented personnel staffing needs. 展开更多
关键词 Budgets Critical CARE Economics Humans Intensive CARE units PERSONNEL hospital PERSONNEL STAFFING and scheduling PHYSICIANS WORKLOAD Quality of health CARE
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核转录因子κB相关蛋白在特发性眼眶炎性假瘤细胞中的表达变化及其意义 预览
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作者 杨林麒 赵鹏翔 +6 位作者 吴亚楠 张旭娟 商蕾 刘梦昱 刘骁 马建民 马雪梅 《中华实验眼科杂志》 CSCD 北大核心 2017年第9期786-791,共6页
背景特发性眼眶炎性假瘤(IOIP)是常见的眼眶疾病之一,症状严重,治疗后易复发,其发病机制尚不清楚。研究表明,核转录因子κB(NF-κB)相关蛋白参与炎症反应、免疫应答以及细胞的重要病理生理过程,其在IOIP的发生过程中是否发挥... 背景特发性眼眶炎性假瘤(IOIP)是常见的眼眶疾病之一,症状严重,治疗后易复发,其发病机制尚不清楚。研究表明,核转录因子κB(NF-κB)相关蛋白参与炎症反应、免疫应答以及细胞的重要病理生理过程,其在IOIP的发生过程中是否发挥作用值得关注。目的探讨NF-κB信号通路在IOIP发病机制中的作用。方法于2010年9月至2016年5月收集在北京同仁医院眼科手术切除并经组织病理学证实的IOIP组织标本24例,制备石蜡切片,采用苏木精一伊红染色法检查IOIP的组织病理学特征,采用免疫组织化学法检测和定位IOIP细胞中NF-κB相关蛋白NF-κB/p65、p-p65、p50和κB抑制蛋白α(IκB-α)的表达,采用免疫细胞化学法及Westernblot法对免疫组织化学检测结果进行验证。结果IOIP的病理组织学特征为以小淋巴细胞为主的多种炎性细胞浸润及不同程度的纤维结缔组织增生,病灶涉及泪腺时可见大量上皮样细胞。免疫组织化学法检测显示,24例IOIP标本的细胞质中均可见NF-κB/p65阳性表达,其中15例可见细胞核中NF-κB/p65呈阳性表达,占62.5%;22例IOIP标本的细胞质中p50呈阳性表达,占91.7%,其中17例细胞核中p50呈阳性表达,占70.8%;22例IOIP标本中p-p65呈阳性表达,占91.7%;11例IOIP标本中IκB-α呈阳性表达,均定位于细胞质,占45.8%。免疫细胞化学法及Westernblot结果与免疫组织化学法检测结果一致。结论IOIP发病过程中NF-κB信号通路可能被激活,NF-κB信号通路可能参与IOIP的发病机制。 展开更多
关键词 核转录因子 κB/代谢 眼眶炎性假瘤/病理 炎症 免疫组织化学 核蛋白/代谢 信号转导通路
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