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Reactivation of hepatitis B virus infection in patients with hemolymphoproliferative diseases,and its prevention 预览
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作者 Caterina Sagnelli Mariantonietta Pisaturo +3 位作者 Federica Calo Salvatore Martini Evangelista Sagnelli Nicola Coppola 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第26期3299-3312,共14页
Reactivation of hepatitis B virus(HBV)replication is characterized by increased HBV-DNA serum values of about 1 log or by HBV DNA turning positive if previously undetectable in serum,possibly associated with liver dam... Reactivation of hepatitis B virus(HBV)replication is characterized by increased HBV-DNA serum values of about 1 log or by HBV DNA turning positive if previously undetectable in serum,possibly associated with liver damage and seldom life-threatening.Due to HBV reactivation,hepatitis B surface antigen(HBsAg)-negative/anti-HBc-positive subjects may revert to HBsAg-positive.In patients with hemo-lymphoproliferative disease,the frequency of HBV reactivation depends on the type of lymphoproliferative disorder,the individual's HBV serological status and the potency and duration of immunosuppression.In particular,it occurs in 10%-50%of the HBsAg-positive and in 2%-25%of the HBsAg-negative/anti-HBc-positive,the highest incidences being registered in patients receiving rituximab-based therapy.HBV reactivation can be prevented by accurate screening of patients at risk and by a pharmacological prophylaxis with anti-HBV nucleo(t)sides starting 2-3 wk before the beginning of immunosuppressive treatment and covering the entire period of administration of immunosuppressive drugs and a long subsequent period,the duration of which depends substantially on the degree of immunodepression achieved.Patients with significant HBV replication before immunosuppressive therapy should receive anti-HBV nucleo(t)sides as a long-term(may be life-long)treatment.This review article is mainly directed to doctors engaged every day in the treatment of patients with onco-lymphoproliferative diseases,so that they can broaden their knowledge on HBV infection and on its reactivation induced by the drugs with high immunosuppressive potential that they use in the care of their patients. 展开更多
关键词 HEPATITIS B VIRUS REACTIVATION HEPATITIS B VIRUS infection Hemolymphoproliferative DISEASES IMMUNOSUPPRESSIVE THERAPY HEPATITIS B VIRUS THERAPY HEPATITIS B VIRUS prophylasis
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Hepatitis C virus antigens enzyme immunoassay for one-step diagnosis of hepatitis C virus coinfection in human immunodeficiency virus infected individuals 预览
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作者 Ke-Qin Hu Wei Cui +1 位作者 Susan D Rouster Kenneth E Sherman 《世界肝病学杂志:英文版(电子版)》 2019年第5期442-449,共8页
BACKGROUND Current diagnosis of hepatitis C virus(HCV)infection requires two sequential steps:testing for anti-HCV followed by HCV RNA PCR to confirm viremia.We have developed a highly sensitive and specific HCV-antig... BACKGROUND Current diagnosis of hepatitis C virus(HCV)infection requires two sequential steps:testing for anti-HCV followed by HCV RNA PCR to confirm viremia.We have developed a highly sensitive and specific HCV-antigens enzyme immunoassay(HCV-Ags EIA)for one-step diagnosis of viremic HCV infection.AIM To assess the clinical application of the HCV-Ags EIA in one-step diagnosis of viremic HCV infection in human immunodeficiency virus(HIV)-coinfected individuals.METHODS The study blindly tested HCV-Ags EIA for its performance in one-step diagnosing viremic HCV infection in 147 sera:10 without HCV or HIV infection;54 with viremic HCV monoinfection;38 with viremic HCV/HIV coinfection;and 45 with viremic HCV and non-viremic HIV coinfection.RESULTS Upon decoding,it was 100%accordance of HCV-Ags EIA to HCV infection status by HCV RNA PCR test.In five sera with HCV infection,HCV RNA was as low as 50-59 IU/mL,and four out of five tested positive for HCV-Ags EIA.Likewise,it was also 100%accordance of HCV-Ags EIA to HCV infection status by HCV RNA PCR in 83 sera with HCV and HIV coinfection,regardless if HIV infection was active or not.CONCLUSION The modified HCV-Ags EIA has a lower detection limit equivalent to serum HCV RNA levels of approximately 100 IU/mL.It is highly sensitive and specific in the setting of HIV coinfection,regardless of HIV infection status and CD4 count.These data support the clinical application of the HCV-Ags EIA in one-step diagnosis of HCV infection in HIV-infected individuals. 展开更多
关键词 HEPATITIS C VIRUS HEPATITIS C VIRUS ANTIGENS HEPATITIS C VIRUS core antigen HEPATITIS C VIRUS DIAGNOSTIC test DIAGNOSTIC assay Enzyme immunoassay
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Characterization of hepatitis B virus X gene quasispecies complexity in mono-infection and hepatitis delta virus superinfection 预览
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作者 Cristina Godoy David Tabernero +13 位作者 Sara Sopena Josep Gregori Maria Francesca Cortese Carolina González Rosario Casillas Marcal Yll Ariadna Rando Rosa López-Martínez Josep Quer Gloria González-Aseguinolaza Rafael Esteban Mar Riveiro-Barciela Maria Buti Francisco Rodríguez-Frías 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第13期1566-1579,共14页
BACKGROUND Hepatitis delta virus (HDV) seems to strongly suppress hepatitis B virus (HBV) replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution o... BACKGROUND Hepatitis delta virus (HDV) seems to strongly suppress hepatitis B virus (HBV) replication, although little is known about the mechanism of this interaction. Both these viruses show a dynamic distribution of mutants, resulting in viral quasispecies. Next-generation sequencing is a viable approach for analyzing the composition of these mutant spectra. As the regulatory hepatitis B X protein (HBx) is essential for HBV replication, determination of HBV X gene (HBX) quasispecies complexity in HBV/HDV infection compared to HBV monoinfection may provide information on the interactions between these two viruses.AIM To compare HBV quasispecies complexity in the HBX 5’ region between chronic hepatitis delta (CHD) and chronic HBV mono-infected patients. METHODS Twenty-four untreated patients were included: 7/24 (29.2%) with HBeAgnegative chronic HBV infection (CI, previously termed inactive carriers), 8/24 (33.3%) with HBeAg-negative chronic hepatitis B (CHB) and 9/24 (37.5%) with CHD. A serum sample from each patient was first tested for HBV DNA levels. The HBX 5’ region [nucleotides (nt) 1255-1611] was then PCR-amplified for subsequent next-generation sequencing (MiSeq, Illumina, United States). HBV quasispecies complexity in the region analyzed was evaluated using incidencebased indices (number of haplotypes and number of mutations), abundancebased indices (Hill numbers of order 1 and 2), and functional indices (mutation frequency and nucleotide diversity). We also evaluated the pattern of nucleotide changes to investigate which of them could be the cause of the quasispecies complexity. RESULTS CHB patients showed higher median HBV-DNA levels [5.4 logIU/mL, interquartile range (IQR) 3.5-7.9] than CHD (3.4 logIU/mL, IQR 3-7.6)(P = n.s.) or CI (3.2 logIU/mL, IQR 2.3-3.5)(P < 0.01) patients. The incidence and abundance indices indicated that HBV quasispecies complexity was significantly greater in CI than CHB. A similar trend was observed in CHD patients, although only Hill numbers of order 2 showed statisti 展开更多
关键词 HEPATITIS B VIRUS HEPATITIS DELTA VIRUS HEPATITIS B X gene Next-generation sequencing Viral QUASISPECIES HEPATITIS B virus-hepatitis DELTA VIRUS interaction
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Human immunodeficiency virus and hepatotropic viruses comorbidities as the inducers of liver injury progression 预览
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作者 Murali Ganesan Larisa Y Poluektova +1 位作者 Kusum K Kharbanda Natalia A Osna 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第4期398-410,共13页
Hepatotropic viruses induced hepatitis progresses much faster and causes more liver-related health problems in people co-infected with human immunodeficiency virus(HIV).Although treatment with antiretroviral therapy h... Hepatotropic viruses induced hepatitis progresses much faster and causes more liver-related health problems in people co-infected with human immunodeficiency virus(HIV).Although treatment with antiretroviral therapy has extended the life expectancy of people with HIV,liver disease induced by hepatitis B virus(HBV)and hepatitis C virus(HCV)causes significant numbers of non-acquired immune deficiency syndrome(AIDS)-related deaths in coinfected patients.In recent years,new insights into the mechanisms of accelerated fibrosis and liver disease progression in HIV/HCV and HIV/HBV co-infections have been reported.In this paper,we review recent studies examining the natural history and pathogenesis of liver disease in HIV-HCV/HBV co-infection in the era of direct acting antivirals(DAA)and antiretroviral therapy(ART).We also review the novel therapeutics for management of HIV/HCV and HIV/HBV coinfected individuals. 展开更多
关键词 Human IMMUNODEFICIENCY VIRUS HEPATITIS C VIRUS HEPATITIS B VIRUS FIBROSIS Stiffness Treatment
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Construction of a replication-competent hepatitis B virus vector carrying secreted luciferase transgene and establishment of new hepatitis B virus replication and expression cell lines 预览
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作者 Jie Ruan Cai-Yan Ping +4 位作者 Shuo Sun Xin Cheng Peng-Yu Han Yin-Ge Zhang Dian-Xing Sun 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第39期5961-5972,共12页
BACKGROUND Previously,we have successfully constructed replication-competent hepatitis B virus(HBV)vectors by uncoupling the P open reading frame(ORF)from the preC/C ORF to carefully design the transgene insertion sit... BACKGROUND Previously,we have successfully constructed replication-competent hepatitis B virus(HBV)vectors by uncoupling the P open reading frame(ORF)from the preC/C ORF to carefully design the transgene insertion site to overcome the compact organization of the HBV genome and maintain HBV replication competence.Consequently,the replication-competent HBV vectors carrying foreign genes,including pCH-BsdR,carrying blasticidin resistance gene(399 bp),and pCH-hrGFP,carrying humanized renilla green fluorescent protein gene(720 bp),were successfully obtained.However,the replication efficiency of the former is higher but it is tedious to use,while that of the latter is poor and cannot be quantified.Hence,we need to search for a new reporter gene that is convenient and quantifiable for further research.AIM To establish a helpful tool for intracellular HBV replication and anti-viral drugs screening studies.METHODS We utilized the replication-competent HBV viral vectors constructed by our laboratory,combined with the secreted luciferase reporter gene,to construct replication-competent HBV vectors expressing the reporter gene secretory Nanoluc Luciferase(SecNluc).HepG2.TA2-7 cells were transfected with this vector to obtain cell lines with stably secreted HBV particles carrying secNluc reporter gene.RESULTS The replication-competent HBV vector carrying the SecNluc reporter gene pCHsNLuc could produce all major viral RNAs and a full set of envelope proteins and achieve high-level secreted luciferase expression.HBV replication intermediates could be produced from this vector.Via transfection with pTRE-sNLuc and selection by hygromycin,we obtained isolated cell clones,named HBV-NLuc-35 cells,which could secrete secNLuc recombinant viruses,and were sensitive to existing anti-HBV drugs.Using differentiated HepaRG cells,it was verified that recombinant HBV possessed infectivity.CONCLUSION Our research demonstrated that a replication-competent HBV vector carrying a secreted luciferase transgene possesses replication and expression 展开更多
关键词 Hepatitis B virus Replication-competent hepatitis B virus vector Secreted luciferase gene Hepatitis B virus cell line
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Viral Regulation of RNA Granules in Infected Cells
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作者 Qiang Zhang Nishi R. Sharma +1 位作者 Zhi-Ming Zheng Mingzhou Chen 《中国病毒学:英文版》 CAS CSCD 2019年第2期175-191,共17页
RNA granules are cytoplasmic, microscopically visible, non-membrane ribo-nucleoprotein structures and are important posttranscriptional regulators in gene expression by controlling RNA translation and stability. TIA/G... RNA granules are cytoplasmic, microscopically visible, non-membrane ribo-nucleoprotein structures and are important posttranscriptional regulators in gene expression by controlling RNA translation and stability. TIA/G3BP/PABP-specific stress granules(SG) and GW182/DCP-specific RNA processing bodies(PB) are two major distinguishable RNA granules in somatic cells and contain various ribosomal subunits, translation factors, scaffold proteins, RNA-binding proteins, RNA decay enzymes and helicases to exclude m RNAs from the cellular active translational pool. Although SG formation is inducible due to cellular stress, PB exist physiologically in every cell. Both RNA granules are important components of the host antiviral defense. Virus infection imposes stress on host cells and thus induces SG formation. However, both RNA and DNA viruses must confront the hostile environment of host innate immunity and apply various strategies to block the formation of SG and PB for their effective infection and multiplication. This review summarizes the current research development in the field and the mechanisms of how individual viruses suppress the formation of host SG and PB for virus production. 展开更多
关键词 Stress GRANULES (SG) P-bodies (PB) RNA VIRUS - DNA VIRUS
MicroRNA-135a Modulates Hepatitis C Virus Genome Replication through Downregulation of Host Antiviral Factors
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作者 Catherine Sodroski Brianna Lowey +2 位作者 Laura Hertz T. Jake Liang Qisheng Li 《中国病毒学:英文版》 CAS CSCD 2019年第2期197-210,共14页
Cellular microRNAs(miRNAs) have been shown to modulate HCV infection via directly acting on the viral genome or indirectly through targeting the virus-associated host factors. Recently we generated a comprehensive map... Cellular microRNAs(miRNAs) have been shown to modulate HCV infection via directly acting on the viral genome or indirectly through targeting the virus-associated host factors. Recently we generated a comprehensive map of HCV–miRNA interactions through genome-wide miRNA functional screens and transcriptomics analyses. Many previously unappreciated cellular miRNAs were identified to be involved in HCV infection, including miR-135a, a human cancerrelated miRNA. In the present study, we investigated the role of miR-135a in regulating HCV life cycle and showed that it preferentially enhances viral genome replication. Bioinformatics-based integrative analyses and subsequent functional assays revealed three antiviral host factors, including receptor interacting serine/threonine kinase 2(RIPK2), myeloid differentiation primary response 88(MYD88), and C-X-C motif chemokine ligand 12(CXCL12), as bona fide targets of miR-135a. These genes have been shown to inhibit HCV infection at the RNA replication stage. Our data demonstrated that repression of key host restriction factors mediated the proviral effect of miR-135a on HCV propagation. In addition,miR-135a hepatic abundance is upregulated by HCV infection in both cultured hepatocytes and human liver, likely mediating a more favorable environment for viral replication and possibly contributing to HCV-induced liver malignancy.These results provide novel insights into HCV–host interactions and unveil molecular pathways linking miRNA biology to HCV pathogenesis. 展开更多
关键词 Hepatitis C virus (HCV) GENOME REPLICATION Virus-host interactions miR-135a ANTIVIRAL factors
Towards the worldwide eradication of hepatitis B virus infection:A combination of prophylactic and therapeutic factors 预览
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作者 Caterina Sagnelli Evangelista Sagnelli 《世界临床传染病学杂志》 2019年第2期11-22,共12页
Hepatitis B virus(HBV)is still a global health problem,mostly because of the intermediate/high rates of HBV chronic carriers living in most Asian,African and eastern European countries.The universal HBV vaccination of... Hepatitis B virus(HBV)is still a global health problem,mostly because of the intermediate/high rates of HBV chronic carriers living in most Asian,African and eastern European countries.The universal HBV vaccination of new-borns undertaken in most nations over the last 3 decades and effective HBV antiviral treatments(nucleos(t)ide analogue with high genetic barrier to viral resistance)introduced in the last decade have shown their beneficial effects in inducing a clear reduction of HBV endemicity in the countries where they have been extensively applied.Great hopes are now placed on new antiviral and immunotherapeutic drugs that are now at an advanced stage of study.It is in fact already conceivable that the synergistic use of new drugs targeting more than one HBV-lifecycle steps(covalent closed circular DNA destruction/silencing,HBV entry inhibitors,nucleocapsid assembly modulators targeting viral transcripts)and of some new immunotherapeutic agents might eliminate the intrahepatic covalent closed circular DNA and achieve the eradication of HBV infection.In spite of this,a strong effort should be given to extensive educational and screening programs for the at-risk population and to the implementation of HBV vaccination in developing countries. 展开更多
关键词 HEPATITIS B VIRUS Chronic HEPATITIS B infection HEPATITIS B VIRUS prevention VACCINATION
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Establishment of Novel Monoclonal Fabs Specific for Epstein-Barr Virus Encoded Latent Membrane Protein 1
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作者 Gaoxin Li Ling Ding +3 位作者 Xiaojing Ma Qiliang Cai Tianlei Ying Fang Wei 《中国病毒学:英文版》 CAS CSCD 2019年第4期467-470,共4页
Dear Editor,Epstein-Barr virus(EBV,also termed human herpesvirus-4)was the first identified human tumor virus.Since its discovery in 1964,studies have shown that EBV infects over 90%of all people by the time they are ... Dear Editor,Epstein-Barr virus(EBV,also termed human herpesvirus-4)was the first identified human tumor virus.Since its discovery in 1964,studies have shown that EBV infects over 90%of all people by the time they are adults(Williams and Crawford 2006).EBV infection can result in mucocutaneous and systemic diseases,ranging from selflimited illnesses to aggressive malignancies,including B cell Hodgkin lymphoma and nasopharyngeal carcinoma.In vitro,EBV transforms resting B cells into proliferating blast cells(Pope et al.1968). 展开更多
关键词 EPSTEIN-BARR VIRUS Encoded Latent Membrane Protein 1 NOVEL MONOCLONAL Fabs SPECIFIC EPSTEIN-BARR VIRUS
长链非编码RNA在病毒与宿主相互作用中功能的研究进展
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作者 宁珊珊 桓晨 张文艳 《中华实验和临床病毒学杂志》 CAS CSCD 2019年第3期332-336,共5页
长链非编码RNA(long non-coding RNA,IncRNA)在细胞生命活动中扮演着重要的角色,在病毒与宿主相互作用中也发挥多种调控作用。病毒的感染能诱导细胞内多种IncRNA的差异表达,而差异表达的IncRNA又可通过各种方式来对侵染的病毒起到或抑... 长链非编码RNA(long non-coding RNA,IncRNA)在细胞生命活动中扮演着重要的角色,在病毒与宿主相互作用中也发挥多种调控作用。病毒的感染能诱导细胞内多种IncRNA的差异表达,而差异表达的IncRNA又可通过各种方式来对侵染的病毒起到或抑制或协助的作用。本文主要列举了不同种类的病毒所诱导的差异表达的IncRNA,以及病毒与这些IncRNA之间的相互作用机制。 展开更多
关键词 长链非编码RNA 病毒 病毒与宿主相互作用
Clinical factors associated with hepatitis B screening and vaccination in high-risk adults 预览
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作者 Rotimi Ayoola Sebastian Larion +1 位作者 David M Poppers Renee Williams 《世界肝病学杂志:英文版(电子版)》 2019年第1期86-98,共13页
BACKGROUND Hepatitis B virus is a viral infection that can lead to acute and/or chronic liver disease, and hepatocellular carcinoma (HCC). Hepatitis B vaccination is 95% effective in preventing infection and the devel... BACKGROUND Hepatitis B virus is a viral infection that can lead to acute and/or chronic liver disease, and hepatocellular carcinoma (HCC). Hepatitis B vaccination is 95% effective in preventing infection and the development of chronic liver disease and HCC due to hepatitis B. In 2011, the Centers for Disease Control updated their guidelines recommending that adults at high-risk for hepatitis B infection be vaccinated against hepatitis B including those with diabetes mellitus (DM). We hypothesize that adults at high-risk for hepatitis B infection are not being adequately screened and/or vaccinated for hepatitis B in a large urban healthcare system. AIM To investigate clinical factors associated with Hepatitis B screening and vaccination in patients at high-risk for Hepatitis B infection. METHODS We conducted a retrospective review of 999 patients presenting at a large urban healthcare system from 2012-2017 at high-risk for hepatitis B infection. Patients were considered high-risk for hepatitis B infection based on hepatitis B practice recommendations from the Center for Disease Control. Medical history including hepatitis B serology, concomitant medical diagnoses, demographics, insurance status and social history were extracted from electronic health records. Multivariate logistic regression was used to identify clinical risk factors independently associated with hepatitis B screening and vaccination. RESULTS Among the 999 patients, 556 (55.7%) patients were screened for hepatitis B. Of those who were screened, only 242 (43.5%) patients were vaccinated against hepatitis B. Multivariate regression analysis revealed end-stage renal disease [odds ratio (OR): 5.122; 2.766-9.483], alcoholic hepatitis (OR: 3.064; 1.020-9.206), and cirrhosis or end-stage liver disease (OR: 1.909; 1.095-3.329); all P < 0.05 were associated with hepatitis B screening, while age (OR: 0.785; 0.680-0.906), insurance status (0.690; 0.558-0.854), history of DM (OR: 0.518; 0.364-0.737), and human immunodeficiency virus (OR: 0.443; 0.273-0.718); 展开更多
关键词 Health prevention VACCINATION Hepatitis B VIRUS SCREENING Diabetes mellitus Cirrhosis END-STAGE renal disease Human IMMUNODEFICIENCY VIRUS Intravenous drug users
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Risk factors for ribavirin treatment failure in Asian organ transplant recipients with chronic hepatitis E infection 预览
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作者 En Xian Sarah Low Edhel Tripon +11 位作者 Kieron Lim Poh Seng Tan How Cheng Low Yock Young Dan Yin Mei Lee Mark Muthiah Wai Mun Loo Calvin Jianyi Koh Wah Wah Phyo JunXiong Pang Seng Gee Lim Guan-Huei Lee 《世界肝病学杂志:英文版(电子版)》 2019年第6期553-561,共9页
BACKGROUND Hepatitis E virus(HEV)infection is a cause of chronic hepatitis in immunosuppressed patients.Sustained virologic response rates to a 12-wk course of ribavirin therapy were reported to be>70%in the West.T... BACKGROUND Hepatitis E virus(HEV)infection is a cause of chronic hepatitis in immunosuppressed patients.Sustained virologic response rates to a 12-wk course of ribavirin therapy were reported to be>70%in the West.This study describes the outcome of HEV treatment in a transplant center in Singapore.AIM To study the outcome of ribavirin treatment in a series of chronic HEV patients,and the cause of treatment failure.METHODS We studied all of the transplant recipients who were diagnosed with HEV infection between 2012 to 2015.The outcome of therapy and virologic relapse are monitored for three years after the end of therapy.RESULTS Ten transplant recipients(4 liver,5 kidney,and 1 bone marrow transplantation)with positive HEV RNA were studied.Nine patients received at least 12 wk of ribavirin therapy,and the remaining patient resolved after reducing immunosuppression therapy.Two subjects had prolonged viremia that lasted more than one year,despite continuous ribavirin therapy.Four ribavirin-treated patients(44.4%)had HEV RNA relapse after achieving a virologic response by the end of treatment.The overall failure rate is 66.7%.Being a kidney transplant recipient is the strongest risk factor for not achieving an initial sustained virologic response(0/5 treated,Chi-Square test,P<0.05).The most common side effect of ribavirin is anemia(100%)(haemoglobin reduction of 3-6.2 g/dL).Seven patients required either a blood transfusion or erythropoietin therapy.CONCLUSION The sustained virologic response rate of 12-wk ribavirin therapy for HEV infection in this Asian series was lower than expected.Kidney transplant recipients had a higher rate of treatment failure due to higher immunosuppression requirements and adverse effects. 展开更多
关键词 Toxicity ANTIVIRAL agents Hepatitis E VIRUS VIRUS classification Systemic immunity Immune responses PERSISTENT INFECTION
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2014-2017年北京市怀柔区急性呼吸道病毒感染病例流行病学分析
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作者 赵小娟 李超 +1 位作者 姬莉莉 喻金玉 《中国预防医学杂志》 CAS CSCD 2019年第7期608-612,共5页
目的了解怀柔地区呼吸道病毒感染的病原学和流行病学特征,为临床诊断和治疗提供参考。方法选取2014年8月至2017年12月北京怀柔医院就诊的门诊或住院呼吸道感染患者的咽拭子,应用实时荧光PCR方法对8种呼吸道病毒(包括亚型共14种)进行检... 目的了解怀柔地区呼吸道病毒感染的病原学和流行病学特征,为临床诊断和治疗提供参考。方法选取2014年8月至2017年12月北京怀柔医院就诊的门诊或住院呼吸道感染患者的咽拭子,应用实时荧光PCR方法对8种呼吸道病毒(包括亚型共14种)进行检测和分析。结果对911份样本进行检测,检出阳性样本244例,阳性率26.78%;病毒感染率前五位的依次为季节性甲型H3N2流感病毒71份(7.79%)、乙型流感病毒34份(3.73%)、副流感病毒31份(3.40%)、鼻病毒25份(2.74%)、呼吸道合胞病毒23份(2.52%)。≤14岁儿童中呼吸道病毒阳性检出率较高,其中2~岁组阳性率最高,为35.66%,不同年龄组人群呼吸道病毒检出率差异有统计学意义(χ~2=21.31,P=0.00)。不同季节吸道病毒检出率不同(χ~2=43.96,P<0.01),冬季最高(42.41%),其次是春季和秋季。流感病毒、RSV和人偏肺病毒感染主要发生在每年的冬春季,PIV感染主要发生在夏秋季。呼吸道病毒在上呼吸道感染病例(31.76%)中的检出率高于下呼吸道感染病例(24.71%)(χ~2=4.44,P=0.04)。结论 14岁及以下儿童呼吸道病毒感染率较高,是防控的重点人群;呼吸道病毒感染呈现一定的季节性特征,冬春季高发。 展开更多
关键词 呼吸道感染 病毒 病原学 流行病学 流感 副流感病毒
miRNA调控昆虫与病毒互作的研究进展 预览
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作者 鲁莎 郭建洋 +1 位作者 席羽 万方浩 《生物安全学报》 CSCD 2019年第2期89-94,共6页
miRNA是一类重要的非编码小分子RNA,可在转录后水平调控基因表达,参与并调控机体的生长发育、细胞分化、细胞凋亡、抗病毒、激素分泌、神经系统等重要生物过程。本文介绍了miRNA的合成途径及其生物学功能,并重点阐述miRNA在昆虫宿主与... miRNA是一类重要的非编码小分子RNA,可在转录后水平调控基因表达,参与并调控机体的生长发育、细胞分化、细胞凋亡、抗病毒、激素分泌、神经系统等重要生物过程。本文介绍了miRNA的合成途径及其生物学功能,并重点阐述miRNA在昆虫宿主与病毒互作中的调控作用:通过mRNA剪切或抑制靶标蛋白的翻译负调控靶标基因,实现基因沉默,调控约50%的蛋白质编码基因的表达,许多miRNA已被发现在人体和植物中参与调控病毒的复制侵染,因此也有可能控制害虫对病毒抗性的产生,恢复病毒对害虫的防控作用。最近有研究将害虫特异的miRNA转入植物,干扰昆虫蜕皮过程导致幼虫的死亡,作为Bt转基因作物的替代,成为抗虫基因工程的新选择。研究miRNA在昆虫对病毒抗性产生中的作用,将为昆虫抗病毒机制的研究提供新的思路,为害虫生物防治措施的应用及改进提供理论参考。 展开更多
关键词 MIRNA 昆虫 病毒 宿主与病毒互作
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Plant infection by two different viruses induce contrasting changes of vectors fitness and behavior
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作者 Quentin Chesnais Aude Couty +2 位作者 Maryline Uzest Veronique Brault Arnaud Ameline 《昆虫科学:英文版》 SCIE CAS CSCD 2019年第1期86-96,共11页
Insect-vectored plant viruses can induce changes in plant phenotypes,thus influencing plant-vector interactions in a way that may promote their dispersal according to their mode of transmission (i.e.,circulative vs.no... Insect-vectored plant viruses can induce changes in plant phenotypes,thus influencing plant-vector interactions in a way that may promote their dispersal according to their mode of transmission (i.e.,circulative vs.noncirculative).This indirect vector manipulation requires host-virus-vector coevolution and would thus be effective solely in very specific plant-virus-vector species associations.Some studies suggest this manipulation may depend on multiple factors relative to various intrinsic characteristics of vectors such as transmission efficiency.In anintegrative study,we tested the effects of infection of the Brassicaceae Camelina sativa with the noncirculative Cauliflower mosaic virus (CaMV)or the circulative Turnip yellows virus (TuYV)on the host-plant colonization of two aphid species differing in their virus transmission efficiency:the polyphagous Myzus persicae,efficient vector of both viruses,and the Brassicaceae specialist Brevicoryne brassicae,poor vector of TuYV and efficient vector of CaMV.Results confirmed the important role of virus mode of transmission as plant-mediated effects of CaMV on the two aphid species induced negative alterations of feeding behavior (i.e.,decreased phloem sap ingestion)and performance that were both conducive for virus fitness by promoting dispersion after a rapid acquisition.In addition,virus transmission efficiency may also play a role in vector manipulation by viruses as only the responses of the efficient vector to plant-mediated effects of TuYV,that is,enhanced feeding behavior and performances,were favorable to their acquisition and further dispersal.Altogether,this work demonstrated that vector transmission efficiency also has to be considered when studying the mechanisms underlying vector manipulation by viruses.Our results also re- inforce the idea that vector manipulation requires coevolution between plant,virus and vector. 展开更多
关键词 APHID vector CAULIFLOWER mosaic VIRUS electrical penetration graph HOST-PLANT selection life history traits TURNIP YELLOWS VIRUS
Application of Newcastle disease virus in the treatment of colorectal cancer 预览
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作者 Hui Song Li-Ping Zhong +2 位作者 Jian He Yong Huang Yong-Xiang Zhao 《世界临床病例杂志》 2019年第16期2143-2154,共12页
Colorectal cancer (CRC) is one of the main reasons of tumor-related deaths worldwide.At present,the main treatment is surgery,but the results are unsatisfactory,and the prognosis is poor.The majority of patients die d... Colorectal cancer (CRC) is one of the main reasons of tumor-related deaths worldwide.At present,the main treatment is surgery,but the results are unsatisfactory,and the prognosis is poor.The majority of patients die due to liver or lung metastasis or recurrence.In recent years,great progress has been made in the field of tumor gene therapy,providing a new treatment for combating CRC.As oncolytic viruses selectively replicate almost exclusively in the cytoplasm of tumor cells and do not require integration into the host genome,they are safer,more effective and more attractive as oncolytic agents.Newcastle disease virus (NDV) is a natural RNA oncolytic virus.After NDV selectively infects tumor cells,the immune response induced by NDV’s envelope protein and intracellular factors can effectively kill the tumor without affecting normal cells.Reverse genetic techniques make NDV a vector for gene therapy.Arming the virus by inserting various exogenous genes or using NDV in combination with immunotherapy can also improve the anti-CRC capacity of NDV,and good results have been achieved in animal models and clinical treatment trials.This article reviews the molecular biological characteristics and oncolytic mechanism of NDV and discusses in vitro and in vivo experiments on NDV anti-CRC capacity and clinical treatment.In conclusion,NDV is an excellent candidate for cancer treatment,but more preclinical studies and clinical trials are needed to ensure its safety and efficacy. 展开更多
关键词 Newcastle disease VIRUS EXOGENOUS gene Armed VIRUS ONCOLYTIC THERAPY COLORECTAL cancer
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马铃薯茎尖脱毒新方法探析 预览
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作者 左静静 闫贵云 +1 位作者 霍利光 左敏 《山西农业科学》 2019年第9期1537-1539,共3页
为了进一步研究高效的马铃薯茎尖脱毒方法,在对马铃薯脱毒技术进行分析的基础上,就马铃薯脱毒种薯进行室内块茎拔高(50~80cm)培养,切取大茎尖(0.8~1.0cm)消毒、无菌培养,然后切取小茎尖(0.2mm)以及茎尖重复培养,以探索马铃薯脱毒的新方... 为了进一步研究高效的马铃薯茎尖脱毒方法,在对马铃薯脱毒技术进行分析的基础上,就马铃薯脱毒种薯进行室内块茎拔高(50~80cm)培养,切取大茎尖(0.8~1.0cm)消毒、无菌培养,然后切取小茎尖(0.2mm)以及茎尖重复培养,以探索马铃薯脱毒的新方法。结果表明,该方法对马铃薯PVX病毒、PVY病毒、PVA病毒以及PSTVd类病毒具有很好的脱毒效果。 展开更多
关键词 马铃薯 病毒 脱毒
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Varicella-zoster virus as a causative agent of acute retinal necrosis in younger patients
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作者 Hai-Yan Xu Meng-Da Li +3 位作者 Jun-Jie Ye Chan Zhao Yun-Tao Hu Yu Di 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第6期659-663,共5页
Background: Herpes virus is considered to be the pathogen of acute retinal necrosis (ARN) infection. Previous studies have that patients with ARN caused by the varicella-zoster virus (VZV) are often older, and patient... Background: Herpes virus is considered to be the pathogen of acute retinal necrosis (ARN) infection. Previous studies have that patients with ARN caused by the varicella-zoster virus (VZV) are often older, and patients with herpes simplex virus (HSV) induced ARN are considerably younger. However, in our clinical work, we find that VZV is also a pathogen in younger ARN patients. We, therefore, aimed to analyze the common etiology of younger ARN patients. Methods: A retrospective analysis was made of 20 eyes (18 patients) diagnosed as having ARN in the Department of Ophthalmology of Peking Union Medical College Hospital from 2014 to 2016. All patients were reviewed for demographic data, clinical course, clinical manifestations, time from onset to initial physician visit, duration of follow-up, visual acuity at both presentation and final visit, and treatment strategies. A paired t test was used to compare visual acuity between the presenting vision and those of final follow-up. Vitreous or aqueous specimens from 18 eyes of 18 patients were analyzed with multiplex polymerase chain reaction (mPCR)/quantitative PCR (qPCR) and xTAG-liquid chip technology (xTAG-LCT) to determine the causative virus of ARN. Results: Final best visual acuity (BCVA) improved significantly from 1.36±0.95 (median 20/400) to 0.95±0.82 (median 20/100)¢=2.714, P = 0.015) after systemic and intravitreal antiviral treatment combined with or without pars plana vitrectomy. PCR and xTAG-LCT results showed four of the five samples in the younger group (32.2±5.2 years) and 12 of the 13 samples in the senior group (53.6±4.9 years) were positive for VZV, and two of the five samples in the younger group were positive for HSV-1. Conclusions: This study demonstrates that VZV is also a common causative virus for ARN in younger patients. Considering this finding, a systemic antiviral treatment protocol should be immediately changed to intravenous ganciclovir when the patient does not respond to acyclovir before determining the causative virus, especial 展开更多
关键词 Retinal necrosis syndrome Acute VARICELLA ZOSTER VIRUS infection Simplex VIRUS GANCICLOVIR ACYCLOVIR
Rapid and sensitive detection of cucumber mosaic virus by reverse transcription loop-mediated isothermal amplification
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作者 Xiaorui Fan Yanan Du +9 位作者 Youming Cai Yongchun Zhang Xiao Zhao Jieling Liang Dan Yang Qi Zhang Xiaoxia Zhang Wanjing Zhang Yan Xu Kai Zhao 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第2期223-226,共4页
Cucumber mosaic virus(CMV),as a member of Cucumovirus genus,is one of the most common lily viruses and has been reported in many countries [1,2].The CMV-infected plants may show developmental abnormalities of leaves,f... Cucumber mosaic virus(CMV),as a member of Cucumovirus genus,is one of the most common lily viruses and has been reported in many countries [1,2].The CMV-infected plants may show developmental abnormalities of leaves,flowers,and bulbs [1].The symptoms will be extremely severe in plants infected by lily symptomless virus(LSV)[1].So far,CMV has posed a threat to cultivars of lily production.Consequently,it is important to develop an efficient approach to achieve rapid and sensitive detection of CMV.Many visual and serological methods have been reported to detect CMV,such as double antibody sandwich enzyme-linked immunosorbent assay [1],immunosorbent electron microscopy [1],enzyme-linked immunosorbent assay(ELISA)and reverse transcriptase-polymerase chain reaction(RT-PCR)[2].However,these methods are time-consuming and require skilled operator and specialized equipment.Loop-mediated isothermal amplification(LAMP)method provides a possible solution for these problems [3].LAMP was developed by Notomi et al.[4] with high specificity and sensitivity because of Bst DNA polymerase.LAMP method not only is suitable for field and large-scale detection of animal virus with more cost-effective but also could be applied to detect foodborne bacterial pathogens and other viruses with high performances [5].For a naked-eye inspection,SYBR Green I was added to the LAMP reaction products to visualize the results.In this study,we developed a rapid and sensitive LAMP method to detect CMV in lily plants and a simple reverse transcription loop-mediated isothermal amplification(RT-LAMP)to detect field CMV samples in lily bulbs. 展开更多
关键词 CUCUMBER MOSAIC VIRUS TRANSCRIPTION loop-mediated ISOTHERMAL AMPLIFICATION CUCUMBER MOSAIC virus(CMV)
Anti-hepatitis C virus therapy in chronic kidney disease patients improves long-term renal and patient survivals 预览
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作者 Yi-Chun Chen Chung-Yi Li +1 位作者 Shiang-Jiun Tsai Yen-Chun Chen 《世界临床病例杂志》 2019年第11期1270-1281,共12页
BACKGROUND Hepatitis C virus (HCV) infection is a documented risk factor for chronic kidney disease (CKD) and progression to end-stage renal disease (ESRD). However, to date there are no reports on the long-term hard ... BACKGROUND Hepatitis C virus (HCV) infection is a documented risk factor for chronic kidney disease (CKD) and progression to end-stage renal disease (ESRD). However, to date there are no reports on the long-term hard endpoints (ESRD and death) of anti-HCV therapy [interferon-based therapy (IBT) or new direct-acting antivirals] in CKD patients. Direct-acting antivirals are not available in Taiwan’s singlepayer national health insurance database currently released for research. Therefore, we hypothesized that a retrospective analysis of the long-term outcomes of IBT in CKD patients will serve as a proxy for direct-acting antivirals to increase our understanding of progression to ESRD following HCV infection. AIM To evaluate the long-term outcomes (ESRD and death) of anti-HCV therapy, especially IBT, in HCV-infected patients with stage 1-5 CKD. METHODS We analyzed 93894 Taiwanese adults diagnosed with CKD and without HBV infection. Of these, 4.9% were infected with HCV. Of the 4582 HCV-infected CKD patients, 482 (10.5%) received IBT (treated cohort). They were matched 1:4 with 1928 untreated HCV-infected CKD patients (untreated cohort) by propensity scores and year, which further matched 1:2 by propensity scores with 3856 CKD patients without HCV infection (uninfected cohort). All participants were followed until the occurrence of ESRD, death, or the end of 2012. The association between HCV infection, IBT use, and risks of ESRD and death was analyzed using competing risk analysis. RESULTS Taking the uninfected cohort as a reference, the adjusted hazard ratios for ESRD, after adjusting for competing mortality, were 0.34 (0.14-0.84, P = 0.019) and 1.28 (1.03-1.60, P = 0.029) in the treated and untreated cohorts, respectively. The treated cohort had a 29%(0.54-0.92, P = 0.011) decrease in mortality compared to the untreated cohort, in which the mortality was 31%(1.18-1.45, P < 0.001) higher than in the uninfected cohort. The reduced risks of ESRD (0.14, 0.03–0.58, P = 0.007) and death (0.57, 0.41-0.79, P = 0.001) w 展开更多
关键词 Hepatitis C VIRUS Chronic kidney DISEASE END-STAGE RENAL DISEASE Antihepatitis C VIRUS THERAPY COHORT study
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