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Human Brain Slice Culture: A Useful Tool to Study Brain Disorders and Potential Therapeutic Compounds
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作者 Xin-Rui Qi Ronald W.H.Verwer +4 位作者 Ai-Min Bao Rawien A.Balesar Sabina Luchetti Jiang-Ning Zhou Dick F.Swaab 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第2期244-252,共9页
Investigating the pathophysiological mechanisms underlying brain disorders is a priority if novel therapeutic strategies are to be developed. In vivo studies of animal models and in vitro studies of cell lines/primary... Investigating the pathophysiological mechanisms underlying brain disorders is a priority if novel therapeutic strategies are to be developed. In vivo studies of animal models and in vitro studies of cell lines/primary cell cultures may provide useful tools to study certain aspects of brain disorders. However, discrepancies among these studies or unsuccessful translation from animal/cell studies to human/clinical studies often occur, because these models generally represent only some symptoms of a neuropsychiatric disorder rather than the complete disorder. Human brain slice cultures from postmortem tissue or resected tissue from operations have shown that, in vitro, neurons and glia can stay alive for long periods of time, while their morphological and physiological characteristics, and their ability to respond to experimental manipulations are maintained. Human brain slices can thus provide a close representation of neuronal networks in vivo, be a valuable tool for investigation of the basis of neuropsychiatric disorders, and provide a platform for the evaluation of novel pharmacological treatments of human brain diseases.A brain bank needs to provide the necessary infrastructure to bring together donors, hospitals, and researchers who want to investigate human brain slices in cultures of clinically and neuropathologically well-documented material. 展开更多
关键词 Alzheimer’s disease BRAIN bank Brain-derived neurotrophic factor Depression Electrical activity HUMAN BRAIN slice CULTURE NEUROPSYCHIATRIC disorders ORGANOTYPIC CULTURE Postmortem HUMAN BRAIN TISSUE Resected HUMAN BRAIN TISSUE
Brain Banking for Research into Neurodegenerative Disorders and Ageing
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作者 Claire E.Shepherd Holly Alvendia Glenda M.Halliday 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第2期283-288,共6页
Advances in cellular and molecular biology underpin most current therapeutic advances in medicine.Such advances for neurological and neurodegenerative diseases are hindered by the lack of similar specimens. It is beco... Advances in cellular and molecular biology underpin most current therapeutic advances in medicine.Such advances for neurological and neurodegenerative diseases are hindered by the lack of similar specimens. It is becoming increasingly evident that greater access to human brain tissue is necessary to understand both the cellular biology of these diseases and their variation. Research in these areas is vital to the development of viable therapeutic options for these currently untreatable diseases. The development and coordination of human brain specimen collection through brain banks is evolving. This perspective article from the Sydney Brain Bank reviews data concerning the best ways to collect and store material for different research purposes. 展开更多
关键词 BRAIN BANKING BRAIN DONATION NEURODEGENERATIVE DISEASES Human BRAIN TISSUE processing
脑科学研究——生命科学最大的挑战 预览
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作者 孙涛 李信晓 《宁夏医科大学学报》 2019年第1期1-6,共6页
随着社会、经济的发展及人们认知的提高,脑科学研究已成为全世界科学研究的热点之一。新兴技术的出现极大促进了脑科学研究,为揭示大脑功能、运作机制和治疗脑疾病方面起到了重要作用。通过脑科学的基础研究、临床研究和基于脑研究大数... 随着社会、经济的发展及人们认知的提高,脑科学研究已成为全世界科学研究的热点之一。新兴技术的出现极大促进了脑科学研究,为揭示大脑功能、运作机制和治疗脑疾病方面起到了重要作用。通过脑科学的基础研究、临床研究和基于脑研究大数据信息的挖掘,为类脑研究项目的开展奠定了基础。同时,脑的发育与衰老、学习与记忆、认知与情绪、意识与精神及各脑区的结构功能研究成为人类不断认识世界、认识自我、超越自我的最终挑战。脑科学研究被称为生命科学最后的疆域,是现代认知科学乃至整个生命科学面临的最大挑战,因此,脑科学研究对提高人们的健康水平、生活质量和创新能力具有重要的现实意义。 展开更多
关键词 大脑 人类脑计划 脑区 人工智能
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Acute drivers of neuroinflammation in traumatic brain injury 预览
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作者 Kathryn L.Wofford David J.Loane D.Kacy Cullen 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1481-1489,共9页
Neuroinflammation is initiated as a result of traumatic brain injury and can exacerbate evolving tissue pathology.Immune cells respond to acute signals from damaged cells,initiate neuroinflammation,and drive the patho... Neuroinflammation is initiated as a result of traumatic brain injury and can exacerbate evolving tissue pathology.Immune cells respond to acute signals from damaged cells,initiate neuroinflammation,and drive the pathological consequences over time.Importantly,the mechanism(s)of injury,the location of the immune cells within the brain,and the animal species all contribute to immune cell behavior following traumatic brain injury.Understanding the signals that initiate neuroinflammation and the context in which they appear may be critical for understanding immune cell contributions to pathology and regeneration.Within this paper,we review a number of factors that could affect immune cell behavior acutely following traumatic brain injury. 展开更多
关键词 traumatic BRAIN INJURY inflammation NEUROINFLAMMATION MICROGLIA macrophage ACUTE diffuse BRAIN INJURY cytokines adenosine 5′-triphosphoate GLUTAMATE calcium
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Decreased numbers of circulating endothelial progenitor cells are associated with hyperglycemia in patients with traumatic brain injury 预览
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作者 Hui-Jie Wei Li Liu +7 位作者 Fang-Lian Chen Dong Wang Liang Wang Zeng-Guang Wang Rong-Cai Jiang Jing-Fei Dong Jie-Li Chen Jian-Ning Zhang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期984-990,共7页
Hyperglycemia reduces the number of circulating endothelial progenitor cells,accelerates their senescence and impairs their function.However,the relationship between blood glucose levels and endothelial progenitor cel... Hyperglycemia reduces the number of circulating endothelial progenitor cells,accelerates their senescence and impairs their function.However,the relationship between blood glucose levels and endothelial progenitor cells in peripheral blood of patients with traumatic brain injury is unclear.In this study,101 traumatic brain injury patients admitted to the Department of Neurosurgery,Tianjin Medical University General Hospital or the Department of Neurosurgery,Tianjin Huanhu Hospital,China,were enrolled from April 2005 to March 2007.The number of circulating endothelial progenitor cells and blood glucose levels were measured at 1,4,7,14 and 21 days after traumatic brain injury by flow cytometry and automatic biochemical analysis,respectively.The number of circulating endothelial progenitor cells and blood sugar levels in 37 healthy control subjects were also examined.Compared with controls,the number of circulating endothelial progenitor cells in traumatic brain injury patients was decreased at 1 day after injury,and then increased at 4 days after injury,and reached a peak at 7 days after injury.Compared with controls,blood glucose levels in traumatic brain injury patients peaked at 1 day and then decreased until 7 days and then remained stable.At 1,4,and 7 days after injury,the number of circulating endothelial progenitor cells was negatively correlated with blood sugar levels(r=?0.147,P<0.05).Our results verify that hyperglycemia in patients with traumatic brain injury is associated with decreased numbers of circulating endothelial progenitor cells.This study was approved by the Ethical Committee of Tianjin Medical University General Hospital,China(approval No.200501)in January 2015. 展开更多
关键词 nerve REGENERATION endothelial progenitor cells VASCULAR repair VASCULAR remodeling angiogenesis NEOVASCULARIZATION blood glucose HYPERGLYCEMIA traumatic BRAIN injury MOBILIZATION suppression senescence alternative therapy BRAIN damage neural REGENERATION
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Vascular endothelial growth factor A promotes platelet adhesion to collagen Ⅳ and causes early brain injury after subarachnoid hemorrhage 预览
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作者 Zun-Wei Liu Jun-Jie Zhao +1 位作者 Hong-Gang Pang Jin-Ning Song 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1726-1733,共8页
The role of vascular endothelial growth factor A in platelet adhesion in cerebral microvessels in the early stage of subarachnoid hemorrhage remains unclear.In this study,the endovascular puncture method was used to p... The role of vascular endothelial growth factor A in platelet adhesion in cerebral microvessels in the early stage of subarachnoid hemorrhage remains unclear.In this study,the endovascular puncture method was used to produce a rat model of subarachnoid hemorrhage.Then,30 minutes later,vascular endothelial growth factor A antagonist anti-vascular endothelial growth factor receptor 2 antibody,10μg,was injected into the right ventricle.Immunohistochemistry and western blot assay were used to assess expression of vascular endothelial growth factor A,occludin and claudin-5.Immunohistochemical double labeling was conducted to examine co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen.TUNEL was used to detect apoptosis in the hippocampus.Neurological score was used to assess behavioral performance.After subarachnoid hemorrhage,the expression of vascular endothelial growth factor A increased in the hippocampus,while occludin and claudin-5 expression levels decreased.Co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen and the number of apoptotic cells increased,whereas behavioral performance was markedly impaired.After treatment with anti-vascular endothelial growth factor receptor 2 antibody,occludin and claudin-5 expression recovered,while co-expression of GP Ⅰa-Ⅱ integrin and type Ⅳ collagen and the number of apoptotic cells decreased.Furthermore,behavioral performance improved notably.Our findings suggest that increased vascular endothelial growth factor A levels promote platelet adhesion and contribute to early brain injury after subarachnoid hemorrhage.This study was approved by the Biomedical Ethics Committee,Medical College of Xi’an Jiaotong University,China in December 2015. 展开更多
关键词 nerve REGENERATION VASCULAR ENDOTHELIAL GROWTH FACTOR A VASCULAR ENDOTHELIAL GROWTH FACTOR receptor 2 subarachnoid hemorrhage brain injuries platelet adhesion COLLAGEN blood-brain barrier neural REGENERATION
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Collagen-chitosan scaffold impregnated with bone marrow mesenchymal stem cells for treatment of traumatic brain injury 预览
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作者 Feng Yan Ming Li +7 位作者 Hong-Qi Zhang Gui-Lin Li Yang Hua Ying Shen Xun-Ming Ji Chuan-Jie Wu Hong An Ming Ren 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1780-1786,共7页
Combinations of biomaterials and cells can effectively target delivery of cells or other therapeutic factors to the brain to rebuild damaged nerve pathways after brain injury.Porous collagen-chitosan scaffolds were pr... Combinations of biomaterials and cells can effectively target delivery of cells or other therapeutic factors to the brain to rebuild damaged nerve pathways after brain injury.Porous collagen-chitosan scaffolds were prepared by a freeze-drying method based on brain tissue engineering.The scaffolds were impregnated with rat bone marrow mesenchymal stem cells.A traumatic brain injury rat model was established using the 300 g weight free fall impact method.Bone marrow mesenchymal stem cells/collagen-chitosan scaffolds were implanted into the injured brain.Modified neurological severity scores were used to assess the recovery of neurological function.The Morris water maze was employed to determine spatial learning and memory abilities.Hematoxylin-eosin staining was performed to measure pathological changes in brain tissue.Immunohistochemistry was performed for vascular endothelial growth factor and for 5-bromo-2-deoxyuridine(BrdU)/neuron specific enolase and BrdU/glial fibrillary acidic protein.Our results demonstrated that the transplantation of bone marrow mesenchymal stem cells and collagen-chitosan scaffolds to traumatic brain injury rats remarkably reduced modified neurological severity scores,shortened the average latency of the Morris water maze,increased the number of platform crossings,diminished the degeneration of damaged brain tissue,and increased the positive reaction of vascular endothelial growth factor in the transplantation and surrounding areas.At 14 days after transplantation,increased BrdU/glial fibrillary acidic protein expression and decreased BrdU/neuron specific enolase expression were observed in bone marrow mesenchymal stem cells in the injured area.The therapeutic effect of bone marrow mesenchymal stem cells and collagen-chitosan scaffolds was superior to stereotactic injection of bone marrow mesenchymal stem cells alone.To test the biocompatibility and immunogenicity of bone marrow mesenchymal stem cells and collagen-chitosan scaffolds,immunosuppressive cyclosporine was intravenously inject 展开更多
关键词 nerve REGENERATION STEM CELLS COLLAGEN chitosan scaffolds traumatic BRAIN injury bone marrow mesenchymal STEM CELLS BRAIN tissue engineering neural REGENERATION
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Differences in pathological changes between two rat models of severe traumatic brain injury 预览
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作者 Yi-Ming Song Yu Qian +6 位作者 Wan-Qiang Su Xuan-Hui Liu Jin-Hao Huang Zhi-Tao Gong Hong-Liang Luo Chuang Gao Rong-Cai Jiang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1796-1804,共9页
The rat high-impact free weight drop model mimics the diffuse axonal injury caused by severe traumatic brain injury in humans,while severe controlled cortical impact can produce a severe traumatic brain injury model u... The rat high-impact free weight drop model mimics the diffuse axonal injury caused by severe traumatic brain injury in humans,while severe controlled cortical impact can produce a severe traumatic brain injury model using precise strike parameters.In this study,we compare the pathological mechanisms and pathological changes between two rat severe brain injury models to identify the similarities and differences.The severe controlled cortical impact model was produced by an electronic controlled cortical impact device,while the severe free weight drop model was produced by dropping a 500 g free weight from a height of 1.8 m through a plastic tube.Body temperature and mortality were recorded,and neurological deficits were assessed with the modified neurological severity score.Brain edema and bloodbrain barrier damage were evaluated by assessing brain water content and Evans blue extravasation.In addition,a cytokine array kit was used to detect inflammatory cytokines.Neuronal apoptosis in the brain and brainstem was quantified by immunofluorescence staining.Both the severe controlled cortical impact and severe free weight drop models exhibited significant neurological impairments and body temperature fluctuations.More severe motor dysfunction was observed in the severe controlled cortical impact model,while more severe cognitive dysfunction was observed in the severe free weight drop model.Brain edema,inflammatory cytokine changes and cortical neuronal apoptosis were more substantial and blood-brain barrier damage was more focal in the severe controlled cortical impact group compared with the severe free weight drop group.The severe free weight drop model presented with more significant apoptosis in the brainstem and diffused blood-brain barrier damage,with higher mortality and lower repeatability compared with the severe controlled cortical impact group.Severe brainstem damage was not found in the severe controlled cortical impact model.These results indicate that the severe controlled cortical impact model is relat 展开更多
关键词 nerve REGENERATION severe traumatic brain INJURY animal model comparison free weight drop controlled cortical impact NEUROLOGICAL impairment NEUROINFLAMMATION blood-brain barrier damage neuronal apoptosis diffuse AXONAL INJURY BRAINSTEM INJURY neural REGENERATION
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Traumatic axonal injury of the cingulum in patients with mild traumatic brain injury:a diffusion tensor tractography study 预览
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作者 Sung Ho Jang Seong Ho Kim Han Do Lee 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1556-1561,共6页
The cingulum,connecting the orbitofrontal cortex to the medial temporal lobe,involves in diverse cognition functions including attention,memory,and motivation.To investigate the relationship between the cingulum injur... The cingulum,connecting the orbitofrontal cortex to the medial temporal lobe,involves in diverse cognition functions including attention,memory,and motivation.To investigate the relationship between the cingulum injury and cognitive impairment in patients with chronic mild traumatic brain injury,we evaluated the integrity between the anterior cingulum and the basal forebrain using diffusion tensor tractography in 73 patients with chronic mild traumatic brain injury(39 males,34 females,age 43.29±11.42 years)and 40 healthy controls(22 males,18 females,age 40.11±16.81 years).The patients were divided into three subgroups based on the integrity between the anterior cingulum and the basal forebrain on diffusion tensor tractography:subgroup A(n=19 patients)– both sides of the anterior cingulum were intact;subgroup B(n=36 patients)– either side of the anterior cingulum was intact;and subgroup C(18 patients)– both sides of the anterior cingulum were discontinued.There were significant differences in total Memory Assessment Scale score between subgroups A and B and between subgroups A and C.There were no significant differences in diffusion tensor tractography parameters(fractional anisotropy,apparent diffusion coefficient,and fiber volume)between patients and controls.These findings suggest that the integrity between the anterior cingulum and the basal forebrain,but not diffusion tensor tractography parameter,can be used to predict the cognitive function of patients with chronic mild traumatic brain injury.This study was approved by Yeungnam University Hospital Institutional Review Board(approval No.YUMC-2014-01-425-010)on August 16,2017. 展开更多
关键词 mild traumatic BRAIN injury BRAIN trauma DIFFUSION TENSOR TRACTOGRAPHY DIFFUSION TENSOR imaging cognitive impairment CINGULUM memory Glasgow COMA Scale neural regeneration
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Extract of Yokukansan improves anxiety-like behavior and increases serum brain-derived neurotrophic factor in rats with cerebral ischemia combined with amyloid-β42 peptide
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作者 Ai Nogami-Hara Kaori Kubota +8 位作者 Kotaro Takasaki Takuya Watanabe Nobuaki Egahira Hikari Iba Risako Fujikawa Shutaro Katsurabayashi Funda Bolukbasi Hatip Izzettin Hatip-Al-Khatib Katsunori Iwasaki 《中医杂志:英文版》 SCIE CAS CSCD 2019年第1期50-58,共9页
OBJECTIVE: To examine the effects of Yokukansan(YKS) extract on two endogenous modulators of anxiety, brain-derived neurotrophic factor(BDNF)and serotonin (5-HT)2A receptors pharmacologically, in the ischemic rat mode... OBJECTIVE: To examine the effects of Yokukansan(YKS) extract on two endogenous modulators of anxiety, brain-derived neurotrophic factor(BDNF)and serotonin (5-HT)2A receptors pharmacologically, in the ischemic rat model of dementia.METHODS: The cerebral ischemia(CI) was induced by bilateral occlusion of the vertebral and common carotid arteries(4-vessel occlusion ischemia). The CI was combined with the amyloid-β42 peptide(A42) injected intracerebroventricularly, and referreβdto as CI+Aβ. Anxiety-like behaviors were assessed by elevated plus maze(enclosed arm), light/dark transition test(dark chamber), and open-field test.Wet-dog shakes were induced by the 5-HT2A receptor agonist 2, 5-dimethoxy-4-iodoamphetamine(DOI). The concentration of BDNF in serum was determined by enzyme-linked immuno sorbent assay.RESULTS: CI + Aβ increased anxiety, as demonstrated by the increase of time spent in the enclosed arms and dark chambers, and locomotion in the outer zone of the open field(thigmotaxis). CI + Adecreased the serum concentration of BDNF. YKS reβ-duced the anxiety-like behaviors, suppressed the DOI-induced wet-dog shakes and increased serum BDNF concentrations.CONCLUSION: Our findings suggest that YKS extract improves CI + Aβ-induced anxiety by antagonizing 5-HT2A receptors and increasing BDNF. 展开更多
关键词 Brain ischemia AMYLOID beta-peptides Anxiety BRAIN-DERIVED NEUROTROPHIC factor Receptor SEROTONIN 5-HT2A Yokukansan
MK-801 attenuates lesion expansion following acute brain injury in rats: a meta-analysis 预览
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作者 Nan-Xing Yi Long-Yun Zhou +8 位作者 Xiao-Yun Wang Yong-Jia Song Hai-Hui Han Tian-Song Zhang Yong-Jun Wang Qi Shi Hao Xu Qian-Qian Liang Ting Zhang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1919-1931,共13页
OBJECTIVE: To evaluate the efficacy and safety of MK-801 and its effect on lesion volume in rat models of acute brain injury.DATA SOURCES: Key terms were "stroke","brain diseases","brain injur... OBJECTIVE: To evaluate the efficacy and safety of MK-801 and its effect on lesion volume in rat models of acute brain injury.DATA SOURCES: Key terms were "stroke","brain diseases","brain injuries","brain hemorrhage, traumatic","acute brain injury","dizocilpine maleate","dizocilpine","MK-801","MK801","rat","rats","rattus" and "murine". PubMed, Cochrane library, EMBASE, the China National Knowledge Infrastructure, WanFang database, the VIP Journal Integration Platform(VJIP) and SinoMed databases were searched from their inception dates to March 2018.DATA SELECTION: Studies were selected if they reported the effects of MK-801 in experimental acute brain injury. Two investigators independently conducted literature screening, data extraction, and methodological quality assessments.OUTCOME MEASURES: The primary outcomes included lesion volume and brain edema. The secondary outcomes included behavioral assessments with the Bederson neurological grading system and the water maze test 24 hours after brain injury.RESULTS: A total of 52 studies with 2530 samples were included in the systematic review. Seventeen of these studies had a high methodological quality. Overall, the lesion volume(34 studies, n = 966, MD =-58.31, 95% CI:-66.55 to-50.07;P < 0.00001) and degree of cerebral edema(5 studies, n = 75, MD =-1.21, 95% CI:-1.50 to-0.91;P < 0.00001) were significantly decreased in the MK-801 group compared with the control group. MK-801 improved spatial cognition assessed with the water maze test(2 studies, n = 60, MD =-10.88, 95% CI:-20.75 to-1.00;P = 0.03) and neurological function 24 hours after brain injury(11 studies, n = 335, MD =-1.04, 95% CI:-1.47 to-0.60;P < 0.00001). Subgroup analysis suggested an association of reduction in lesion volume with various injury models(34 studies, n = 966, MD =-58.31, 95% CI:-66.55 to-50.07;P = 0.004). Further network analysis showed that 0–1 mg/kg MK-801 may be the optimal dose for treatment in the middle cerebral artery occlusion animal model.CONCLUSION: MK-801 effectively reduces b 展开更多
关键词 nerve REGENERATION acute BRAIN injury NEUROLOGICAL function spatial cognition water MAZE test LESION volume BRAIN edema rat systematic review META-ANALYSIS neural REGENERATION
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Shuxuetong injection protects cerebral microvascular endothelial cells against oxygen-glucose deprivation reperfusion 预览
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作者 Zuo-Yan Sun Fu-Jiang Wang +6 位作者 Hong Guo Lu Chen Li-Juan Chai Rui-Lin Li Li-Min Hu Hong Wang Shao-Xia Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期783-793,共11页
Shuxuetong injection composed of leech(Hirudo nipponica Whitman)and earthworm(Pheretima aspergillum)has been used for the clinical treatment of acute stroke for many years in China.However,the precise neuroprotective ... Shuxuetong injection composed of leech(Hirudo nipponica Whitman)and earthworm(Pheretima aspergillum)has been used for the clinical treatment of acute stroke for many years in China.However,the precise neuroprotective mechanism of Shuxuetong injection remains poorly understood.Here,cerebral microvascular endothelial cells(bEnd.3)were incubated in glucose-free Dulbecco’s modified Eagle’s medium containing 95%N2/5%CO2 for 6 hours,followed by high-glucose medium containing 95%O2 and 5%CO2 for 18 hours to establish an oxygen-glucose deprivation/reperfusion model.This in vitro cell model was administered Shuxuetong injection at 1/32,1/64,and 1/128 concentrations(diluted 32-,64-,and 128-times).Cell Counting Kit-8 assay was used to evaluate cell viability.A fluorescence method was used to measure lactate dehydrogenase,and a fluorescence microplate reader used to detect intracellular reactive oxygen species.A fluorescent probe was also used to measure mitochondrial superoxide production.A cell resistance meter was used to measure transepithelial resistance and examine integrity of monolayer cells.The fluorescein isothiocyanate-dextran test was performed to examine blood-brain barrier permeability.Real-time reverse transcription polymerase chain reaction was performed to analyze mRNA expression levels of tumor necrosis factor alpha,interleukin-1β,interleukin-6,and inducible nitric oxide synthase.Western blot assay was performed to analyze expression of caspase-3,intercellular adhesion molecule 1,vascular cell adhesion molecule 1,occludin,vascular endothelial growth factor,cleaved caspase-3,B-cell lymphoma 2,phosphorylated extracellular signal-regulated protein kinase,extracellular signal-regulated protein kinase,nuclear factor-κB p65,I kappa B alpha,phosphorylated I kappa B alpha,I kappa B kinase,phosphorylated I kappa B kinase,claudin-5,and zonula occludens-1.Our results show that Shuxuetong injection increases bEnd.3 cell viability and B-cell lymphoma 2 expression,reduces cleaved caspase-3 expression,inhibits produ 展开更多
关键词 nerve REGENERATION SHUXUETONG injection brain MICROVASCULAR endothelial cells oxygen-glucose deprivation/reperfusion tight junction proteins mitochondrial function inflammatory factors blood-brain barrier neuroprotection neural REGENERATION
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The development of brain functional connectivity networks revealed by resting-state functional magnetic resonance imaging 预览
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作者 Chao-Lin Li Yan-Jun Deng +2 位作者 Yu-Hui He Hong-Chang Zhai Fu-Cang Jia 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第8期1419-1429,共11页
Previous studies on brain functional connectivity networks in children have mainly focused on changes in function in specific brain regions, as opposed to whole brain connectivity in healthy children. By analyzing the... Previous studies on brain functional connectivity networks in children have mainly focused on changes in function in specific brain regions, as opposed to whole brain connectivity in healthy children. By analyzing the independent components of activation and network connectivity between brain regions, we examined brain activity status and development trends in children aged 3 and 5 years. These data could provide a reference for brain function rehabilitation in children with illness or abnormal function. We acquired functional magnetic resonance images from 15 3-year-old children and 15 5-year-old children under natural sleep cond让ions. The participants were recruited from five kindergartens in the Nanshan District of Shenzhen City, China. The parents of the participants signed an informed consent form with the premise that they had been fully informed regarding the experimental protocol. We used masked independent component analysis and BrainNet Viewer software to explore the independent components of the brain and correlation connections between brain regions. We identified seven independent components in the two groups of children, including the executive control network, the dorsal attention network, the default mode network, the left frontoparietal network, the right frontoparietal network, the salience network, and the motor network. In the default mode network, the posterior cingulate cortex, medial frontal gyrus, and inferior parietal lobule were activated in both 3- and 5-year-old children, supporting the "three-brain region theory” of the default mode network. In the frontoparietal network, the frontal and parietal gyri were activated in the two groups of children, and functional connectivity was strengthened in 5-year-olds compared with 3-year-olds, although the nodes and network connections were not yet mature. The high-correlation network connections in the default mode networks and dorsal attention networks had been significantly strengthened in 5-year-olds vs. 3-year-olds. Further, the salience 展开更多
关键词 nerve REGENERATION FUNCTIONAL MRI BRAIN network FUNCTIONAL connectivity RESTING-STATE ICA BRAIN development children RESTING-STATE NETWORKS INFANT template standardized neural REGENERATION
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Resting-state network complexity and magnitude changes in neonates with severe hypoxic ischemic encephalopathy 预览
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作者 Hong-Xin Li Min Yu +4 位作者 Ai-Bin Zheng Qin-Fen Zhang Guo-Wei Hua Wen-Juan Tu Li-Chi Zhang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期642-648,共7页
Resting-state functional magnetic resonance imaging has revealed disrupted brain network connectivity in adults and teenagers with cerebral palsy.However,the specific brain networks implicated in neonatal cases remain... Resting-state functional magnetic resonance imaging has revealed disrupted brain network connectivity in adults and teenagers with cerebral palsy.However,the specific brain networks implicated in neonatal cases remain poorly understood.In this study,we recruited 14 termborn infants with mild hypoxic ischemic encephalopathy and 14 term-born infants with severe hypoxic ischemic encephalopathy from Changzhou Children’s Hospital,China.Resting-state functional magnetic resonance imaging data showed efficient small-world organization in whole-brain networks in both the mild and severe hypoxic ischemic encephalopathy groups.However,compared with the mild hypoxic ischemic encephalopathy group,the severe hypoxic ischemic encephalopathy group exhibited decreased local efficiency and a low clustering coefficient.The distribution of hub regions in the functional networks had fewer nodes in the severe hypoxic ischemic encephalopathy group compared with the mild hypoxic ischemic encephalopathy group.Moreover,nodal efficiency was reduced in the left rolandic operculum,left supramarginal gyrus,bilateral superior temporal gyrus,and right middle temporal gyrus.These results suggest that the topological structure of the resting state functional network in children with severe hypoxic ischemic encephalopathy is clearly distinct from that in children with mild hypoxic ischemic encephalopathy,and may be associated with impaired language,motion,and cognition.These data indicate that it may be possible to make early predictions regarding brain development in children with severe hypoxic ischemic encephalopathy,enabling early interventions targeting brain function.This study was approved by the Regional Ethics Review Boards of the Changzhou Children’s Hospital(approval No.2013-001)on January 31,2013.Informed consent was obtained from the family members of the children.The trial was registered with the Chinese Clinical Trial Registry(registration number:ChiCTR1800016409)and the protocol version is 1.0. 展开更多
关键词 nerve REGENERATION NEONATES hypoxic ischemic encephalopathy RESTING-STATE FUNCTIONAL magnetic resonance imaging BRAIN networks SMALL-WORLD organization BRAIN FUNCTIONAL connectivity local efficiency clustering coefficient neural REGENERATION
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新生未成熟大鼠缺氧缺血性脑损伤时微小RNA-200b对缺氧诱导因子1α的调控作用
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作者 高笑妮 杨丽君 +1 位作者 张囡 崔红 《中华新生儿科杂志(中英文)》 CAS 2019年第1期58-62,共5页
目的 探讨新生未成熟大鼠缺氧缺血性脑损伤(hypoxic ischemic brain damage,HIBD)时微小RNA 200b(micro RNA-200b,miR-200b)对缺氧诱导因子1α(hypoxia-inducible factors-1α,HIF-1α)的调控作用,为早产儿HIBD的治疗提供思路。 方法 选... 目的 探讨新生未成熟大鼠缺氧缺血性脑损伤(hypoxic ischemic brain damage,HIBD)时微小RNA 200b(micro RNA-200b,miR-200b)对缺氧诱导因子1α(hypoxia-inducible factors-1α,HIF-1α)的调控作用,为早产儿HIBD的治疗提供思路。 方法 选取3日龄新生Sprague-Dawley(SD)大鼠240只,随机分为单纯缺氧缺血组(HI组)、造模后双侧侧脑室注射miR-200b激动剂组、注射miR-200b拮抗剂组、注射miR-200b激动剂阴性对照剂组、注射miR-200b拮抗剂阴性对照剂组和正常对照组,每组40只。除正常对照组不予特殊处理外,其他各组均建立未成熟新生大鼠HIBD模型。应用实时定量聚合酶链反应检测各组大鼠侧脑室注射后12 h、1 d、3 d和7 d脑组织中HIF-1α的相对表达量,比较HIF-1α表达的变化。 结果 (1)与正常对照组比较,单纯HI组侧脑室注射生理盐水后12 h HIF-1α表达量开始升高,1 d达高峰,差异有统计学意义(P<0.05),随后逐渐下降,7 d与正常对照组接近,差异无统计学意义(P>0.05)。(2)单纯HI组与HI miR-200b激动剂阴性对照组及HI miR-200b拮抗剂阴性对照组HIF-1α表达量比较,差异无统计学意义(P>0.05),miR-200b纳米载体对HIF-1α表达无明显影响。(3)HI miR-200b拮抗剂组HIF-1α持续处于高表达状态,12 h明显高于单纯HI组,差异有统计学意义(P<0.05);注射后1、3、7 d HI miR-200b拮抗剂组与单纯HI组比较,差异无统计学意义(P>0.05)。HI miR-200b激动剂组HIF-1α表达持续低于单纯HI组,1 d时差异有统计学意义(P<0.05)。 结论 miR-200b过度表达抑制了HIF-1α表达,miR-200b低表达可上调HIF-1α表达水平,但作用时间有限。因此miR-200b有可能通过调控HIF-1α的表达参与新生大鼠HIBD后减轻脑损伤的调节。 展开更多
关键词 缺氧缺血 脑损伤 微小RNA-200b 缺氧诱导因子1 Α亚基 大鼠
ASL技术基本原理及在血管性认知障碍中的研究进展 预览
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作者 蒋佩伶 罗天友 《磁共振成像》 CAS 2019年第5期393-396,共4页
血管性认知障碍(vascular cognitive impairment,VCI)是从轻度认知功能障碍到痴呆的一组综合征。动脉自旋标记(arterial spin labeling,ASL)作为一种功能性磁共振成像(functional MRI,fMRI)技术,通过特定脉冲标记动脉血中的氢质子,在不... 血管性认知障碍(vascular cognitive impairment,VCI)是从轻度认知功能障碍到痴呆的一组综合征。动脉自旋标记(arterial spin labeling,ASL)作为一种功能性磁共振成像(functional MRI,fMRI)技术,通过特定脉冲标记动脉血中的氢质子,在不依赖血脑屏障的情况下,能真实反映各个阶段VCI患者脑血流量(cerebral blood flow,CBF),具有良好时间分辨率,已成为诊断和随访VCI的重要影像学手段。作者就ASL技术基本原理及近年来在VCI中的研究进展进行综述。 展开更多
关键词 认知功能障碍 痴呆 血管性 局部血流 磁共振成像
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Neurogenesis in the hippocampus of adult humans: controversy "fixed" at last 预览
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作者 Silene M.A. Lima Walace Gomes-Leal 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1917-1918,共2页
The presence of adult neurogenesis in the mammalian brain has been a theme of intense controversy for a long time since the original report by Altman and Das (1965). The scientific community, for about 30 years, has d... The presence of adult neurogenesis in the mammalian brain has been a theme of intense controversy for a long time since the original report by Altman and Das (1965). The scientific community, for about 30 years, has difficulties to accept that progenitor cells give rise to new neurons in some specific regions of the mammalian adult brain, the neurogenic niches (Kuhn et al., 1996;Doetsch et al., 1997, 1999). 展开更多
关键词 NEUROGENESIS HIPPOCAMPUS MAMMALIAN BRAIN
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Effect of Xylazine Anesthesia on Glu and GABA Amino Acid Neurotransmitters in Rat 预览
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作者 Zhang Xin-tong Li Li-na +6 位作者 Wang Qi Chang Tian Wen Ya-jing Yuan Rui Du Xue-man Tang Sai-nan Gao Li 《东北农业大学学报:英文版》 CAS 2019年第1期46-52,共7页
Neurotransmitters of the central nervous system were the important way to study the mechanism of anesthesia. The effect of different doses of xylazine anesthetic on the glutamate(Glu) and γ-aminobutyric-acid(GABA) we... Neurotransmitters of the central nervous system were the important way to study the mechanism of anesthesia. The effect of different doses of xylazine anesthetic on the glutamate(Glu) and γ-aminobutyric-acid(GABA) were investigated and the mechanism of xylazine anesthetic on the central nervous system were explored in this study. A total of 88 rats were randomly divided into three groups, including normal saline control group, group with low dose of xylazine and group with high dose of xylazine.Cerebrum, cerebellum, hippocampus, thalamus and brainstem were collected. The results showed that the concentration of Glu in the hippocampus, thalamus and brainstem decreased first and then increased, but it increased first and then decreased in the cerebrum and cerebellum during the period of anesthesia. The concentration of GABA in the cerebrum, thalamus, brainstem and hippocampus increased first and then decreased. The results showed that xylazine inhibited Glu and promoted GABA with different dose dependence. The results and methods could provide guides for the clinical use of xylazine. 展开更多
关键词 brain GLUTAMATE γ-aminobutyric acid RAT XYLAZINE
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重组人脑利钠肽治疗高龄心力衰竭患者的疗效观察及其对血清N端B型钠尿肽原水平的影响
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作者 宋添晶 吴玉泉 +1 位作者 郦虹 陈晓娜 《中国基层医药》 CAS 2019年第9期1070-1073,共4页
目的分析重组人脑利钠肽(rhBNP)对高龄心力衰竭患者疗效、心功能及血清N端B型钠尿肽原(NT-proBNP)水平的影响。方法将解放军第一一七医院2015年1月至2017年12月收治的高龄(年龄≥80岁)心力衰竭患者150例作为研究对象,采用随机数字表法... 目的分析重组人脑利钠肽(rhBNP)对高龄心力衰竭患者疗效、心功能及血清N端B型钠尿肽原(NT-proBNP)水平的影响。方法将解放军第一一七医院2015年1月至2017年12月收治的高龄(年龄≥80岁)心力衰竭患者150例作为研究对象,采用随机数字表法分为两组,对照组给予硝酸甘油治疗,观察组给予rhBNP治疗,两组各75例。比较两组患者血清NT-proBNP水平、左心室射血分数(LVEF)、临床疗效及并发症发生情况。结果两组患者治疗后血清NT-proBNP水平较治疗前均显著降低,且观察组血清NT-proBNP水平[(2 964.42±607.25)pg/mL]较对照组的(4 213.57±524.07)pg/mL显著降低(t=13.49,P<0.01)。两组患者治疗后LVEF的水平较治疗前均显著升高,且观察组LVEF水平[(51.26±4.77)%]较对照组的(46.03±5.22)%显著升高(t=6.41,P<0.01)。观察组治疗总有效率为94.67%(71/75),较对照组的70.67%(53/75)显著升高(χ^2=13.45,P<0.01)。两组患者头痛、血压下降、肾功能恶化及心动过速等并发症发生率差异无统计学意义(P>0.05)。结论rhBNP对高龄心力衰竭患者的治疗可有效改善其呼吸困难及全身症状,有效降低血清NT-proBNP水平,改善心功能,且不会引起严重并发症,具有良好的安全性。 展开更多
关键词 心力衰竭 利钠肽 心脏功能试验 老年人 疗效比较研究
Study of brain morphology change in Alzheimer’s disease and amnestic mild cognitive impairment compared with normal controls 预览
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作者 Huanqing Yang Hua Xu +10 位作者 Qingfeng Li Yan Jin Weixiong Jiang Jinghua Wang Yina Wu Wei Li Cece Yang Xia Li Shifu Xiao Feng Shi Tao Wang 《综合精神医学(英文)》 CSCD 2019年第2期75-83,共9页
Background With an aggravated social ageing level, the number of patients with Alzheimer's disease (AD) is gradually increasing, and mild cognitive impairment (MCI) is considered to be an early form of Alzheimer&#... Background With an aggravated social ageing level, the number of patients with Alzheimer's disease (AD) is gradually increasing, and mild cognitive impairment (MCI) is considered to be an early form of Alzheimer's disease. How to distinguish diseases in the early stage for the purposes of early diagnosis and treatment is an important topic. Aims The purpose of our study was to investigate the differences in brain cortical thickness and surface area among elderly patients with AD, elderly patients with amnestic MCI (aMCI) and normal controls (NC). Methods 20 AD patients, 21 aMCIs and 25 NC were recruited in the study. FreeSurfer software was used to calculate cortical thickness and surface area among groups. Results The patients with AD had less cortical thickness both in the left and right hemisphere in 17 of the 36 brain regions examined than the patients with aMCI or NC. The patients with AD also had smaller cerebral surface area both in the left and right hemisphere in 3 of the 36 brain regions examined than the patients with aMCI or NC. Compared with the NC, the patients with aMCI only had slight atrophy in the inferior parietal lobe of the left hemisphere, and no significant difference was found. Conclusion AD, as well as aMCI (to a lesser extent), is associated with reduced cortical thickness and surface area in a few brain regions associated with cognitive impairment. These results suggest that cortical thickness and surface area could be used for early detection of AD. 展开更多
关键词 BRAIN morphology CHANGE amnestic MILD COGNITIVE IMPAIRMENT compared aMCI NC
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