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甲状腺纤维化的中医治疗思路 预览
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作者 柏力萄 赵静 +3 位作者 李俊 吴瑞 魏璠 魏军平 《世界科学技术:中医药现代化》 CSCD 北大核心 2019年第2期267-271,共5页
甲状腺纤维化是各种因素刺激下,出现的纤维性改变,是多种甲状腺疾病共有的病理过程。甲状腺纤维化严重影响患者生存质量,故针对纤维化治疗具有重要意。中药辨证治疗甲状腺纤维化以疏肝行气、健脾补肾、活血化瘀、祛痰除湿、软坚散结为... 甲状腺纤维化是各种因素刺激下,出现的纤维性改变,是多种甲状腺疾病共有的病理过程。甲状腺纤维化严重影响患者生存质量,故针对纤维化治疗具有重要意。中药辨证治疗甲状腺纤维化以疏肝行气、健脾补肾、活血化瘀、祛痰除湿、软坚散结为总原则。根据纤维化早、中、晚三期分别采用半夏厚朴汤,补中益气汤,桂附地黄汤合真武汤加丹参、地龙、贝母等方药治疗。并加入穿山龙、夏枯草、雷公藤、山慈菇、黄药子、人参皂甙等具有抗甲状腺纤维化的中药及单体,以期为临床治疗提供一种新的思路。 展开更多
关键词 甲状腺 纤维化 中医药治疗 抗纤维化
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Current therapies and novel approaches for biliary diseases 预览
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作者 Indu G Rajapaksha Peter W Angus Chandana B Herath 《世界胃肠病理生理学杂志:英文版(电子版)》 2019年第1期1-10,共10页
Chronic liver diseases that inevitably lead to hepatic fibrosis, cirrhosis and/or hepatocellular carcinoma have become a major cause of illness and death worldwide. Among them, cholangiopathies or cholestatic liver di... Chronic liver diseases that inevitably lead to hepatic fibrosis, cirrhosis and/or hepatocellular carcinoma have become a major cause of illness and death worldwide. Among them, cholangiopathies or cholestatic liver diseases comprise a large group of conditions in which injury is primarily focused on the biliary system. These include congenital diseases (such as biliary atresia and cystic fibrosis), acquired diseases (such as primary sclerosing cholangitis and primary biliary cirrhosis), and those that arise from secondary damage to the biliary tree from obstruction, cholangitis or ischaemia. These conditions are associated with a specific pattern of chronic liver injury centered on damaged bile ducts that drive the development of peribiliary fibrosis and, ultimately, biliary cirrhosis and liver failure. For most, there is no established medical therapy and, hence, these diseases remain one of the most important indications for liver transplantation. As a result, there is a major need to develop new therapies that can prevent the development of chronic biliary injury and fibrosis. This mini-review briefly discusses the pathophysiology of liver fibrosis and its progression to cirrhosis. We make a special emphasis on biliary fibrosis and current therapeutic options, such as angiotensin converting enzyme-2 (known as ACE2) over-expression in the diseased liver as a novel potential therapy to treat this condition. 展开更多
关键词 Chronic liver disease BILIARY FIBROSIS CURRENT THERAPIES for BILIARY FIBROSIS ANGIOTENSIN CONVERTING enzyme-2 Gene therapy
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Novel non-invasive score to predict cirrhosis in the era of hepatitis C elimination:A population study of ex-substance users in Singapore 预览
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作者 Yue Zhao Prem Harichander Thurairajah +3 位作者 Rahul Kumar Jessica Tan Eng Kiong Teo John Chen Hsiang 《国际肝胆胰疾病杂志:英文版》 SCIE CAS CSCD 2019年第2期143-148,共6页
Background:Chronic hepatitis C infection is common among people with history of substance use.Liver fibrosis assessment is a barrier to linkage to care,particularly among those with history of substance users.The use ... Background:Chronic hepatitis C infection is common among people with history of substance use.Liver fibrosis assessment is a barrier to linkage to care,particularly among those with history of substance users.The use of non-invasive scores can be helpful in predicting liver cirrhosis in the era of HCV elimination,especially in countries where transient elastography(TE)is not available.We compared the commonly used non-invasive scores with a novel non-invasive score in predicting liver cirrhosis in this population.Methods:HCV patients with history of substance use between 2011 and 2016 were analyzed.All patients had TE for liver fibrosis assessment.Clinical performance of established non-invasive scores for fibrosis assessment and novel score were compared.Youden’s index was used to determine optimal cut-off of the novel score.Results:A total of 579 patients were included.In multivariate logistic regression,cirrhosis on TE was associated with age(P=0.002),aspartate aminotransferase(AST)(P=0.004),and platelet count(P<0.001),but not alanine aminotransferase(ALT)(P=0.896).These form the components of modified AST-toplatelet ratio index(APRI)score.Modified APRI was superior to APRI in predicting cirrhosis(AUROC,0.796 vs.0.770,P=0.007),but not fibrosis-4 score(FIB-4)(P=1.00).Modified APRI at cut-off of 4 has sensitivity,specificity and negative predictive value(NPV)of 94.4%,26.9%and 92.6%,respectively,and at 19,has sensitivity,specificity and positive predictive value(PPV)of 33.3%,96.2%and 77.1%,respectively.FIB-4 has a NPV and PPV of 88.6%,41.8%and 78.5%,77.6%,at cut-off of 1.45 and 3.25,respectively.Using the cut-off of 4 and 14 for modified APRI,32.5%of patients can be correctly classified and misses out only 5.6%of cirrhosis patients.Conclusions:Modified APRI score is superior in predicting cirrhosis in HCV population,with 32.5%of the population being correctly classified using cut-off of 4 and 14.Further studies are required to validate the findings. 展开更多
关键词 Hepatitis C Cirrhosis Fibrosis assessment AST-to-platelet ratio index Modified APRI Fibrosis-4 SCORE
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气管瘢痕狭窄动物模型的研究进展 预览
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作者 王志鹏 张春林 刘兆辉 《海南医学》 CAS 2019年第10期1326-1328,共3页
气管狭窄是气管切开严重的术后并发症,严重影响患者生存质量,临床处理棘手。气管狭窄的发病机制复杂,目前仍未完全清楚,本文将近年来学者关于气管狭窄的动物模型及发生机制的研究做一综述。
关键词 气管狭窄 动物模型 机制 瘢痕 纤维化
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子宫内膜损伤修复与宫腔粘连形成机制研究进展
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作者 郭罗培 隋龙 《中国实用妇科与产科杂志》 CAS CSCD 北大核心 2019年第6期706-709,共4页
宫腔粘连(IUA)又称Asherman Syndrome,自1894年首次由Heinrich Fritsch报道,54年之后,才经以色列妇科医生Joseph Asherman详细介绍了这一疾病,主要表现为闭经、不孕、盆腔疼痛等。同时,他发现子宫内膜损伤极有可能是本病的发病原因[1]... 宫腔粘连(IUA)又称Asherman Syndrome,自1894年首次由Heinrich Fritsch报道,54年之后,才经以色列妇科医生Joseph Asherman详细介绍了这一疾病,主要表现为闭经、不孕、盆腔疼痛等。同时,他发现子宫内膜损伤极有可能是本病的发病原因[1]。那么,宫腔粘连的发病机制与子宫内膜损伤修复有什么联系,具体是如何发生的呢?本文就此问题进行综述。 展开更多
关键词 损伤修复 宫腔粘连 纤维化 上皮间质转化
姜黄素类似物J7对2型糖尿病大鼠肾脏的保护作用 预览
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作者 姬秀焕 章琼莹 +3 位作者 池琛 吴谷 李慧敏 陈国荣 《浙江医学》 CAS 2019年第13期1342-1347,I0004共7页
目的探讨姜黄素(CUR)类似物J7对2型糖尿病(T2DM)大鼠肾脏的保护作用及其可能机制。方法将雄性SD大鼠随机分成5组:正常对照(NC)组、模型组、CUR治疗组、J7低剂量(LJ7)治疗组和J7高剂量(HJ7)治疗组。后4组采用高脂高糖饮食喂养4周,单次腹... 目的探讨姜黄素(CUR)类似物J7对2型糖尿病(T2DM)大鼠肾脏的保护作用及其可能机制。方法将雄性SD大鼠随机分成5组:正常对照(NC)组、模型组、CUR治疗组、J7低剂量(LJ7)治疗组和J7高剂量(HJ7)治疗组。后4组采用高脂高糖饮食喂养4周,单次腹腔注射链脲佐菌素(30mg/kg)诱导T2DM模型。CUR组、LJ7组和HJ7组分别按20mg/kg CUR、10mg/kg J7、20mg/kg J7灌胃8周,1次/d;每次灌胃量按1ml/100g计算得出,其余组予同等容积羧甲基纤维素钠灌胃。在光镜和电镜下观察各组大鼠肾脏组织形态改变;测量各组大鼠体重、肾重,计算肾重指数;检测各组大鼠空腹血糖(FBG)、血肌酐(Scr)、尿素氮(BUN)水平;硫代巴比妥酸法检测各组大鼠肾脏组织丙二醛(MDA)水平;羟胺法检测各组大鼠肾脏组织超氧化物歧化酶(SOD)活性;免疫组化染色法检测各组大鼠肾脏转化生长因子β1(TGF-β1)蛋白表达;Western blot法检测各组大鼠肾脏组织TGF-β1、人信号转导因子7(Smad7)、B淋巴细胞瘤-2基因相关X蛋白(Bax)、B淋巴细胞瘤-2基因(Bcl-2)蛋白相对表达量及Bcl-2/Bax。结果与NC组比较,模型组大鼠肾小球肥大,胶原纤维增多,细胞外基质沉积,足突融合;体重、SOD活性和Bcl-2、Smad7蛋白相对表达量及Bcl-2/Bax均明显降低(均P<0.05),肾重指数、FBG、Scr、BUN、MDA水平和Bax、TGF-β1蛋白相对表达量均明显升高(均P<0.05);经CUR或J7治疗后,相应病理学改变减轻,体重略有上升,SOD活性和Bcl-2、Smad7蛋白相对表达量及Bcl-2/Bax均明显升高(均P<0.05),FBG、MDA水平和Bax、TGF-β1蛋白相对表达量均明显降低(均P<0.05)。结论CUR类似物J7对T2DM大鼠的肾脏具有保护作用,可能与其具有抑制氧化应激、抗细胞凋亡、抑制TGF-β/Smad通路及纤维化作用有关。 展开更多
关键词 糖尿病 肾脏 姜黄素类似物J7 氧化应激 纤维化
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Effect of NLRC5 on activation and reversion of hepatic stellate cells by regulating the nuclear factor-κB signaling pathway 预览
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作者 Yan-Zhen Zhang Jian-Ning Yao +3 位作者 Lian-Feng Zhang Chun-Feng Wang Xue-Xiu Zhang Bing Gao 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第24期3044-3055,共12页
BACKGROUND The formation of liver fibrosis is mainly caused by the activation of hepatic stellate cells(HSCs)and the imbalance of extracellular matrix(ECM)production and degradation.The treatment of liver fibrosis mai... BACKGROUND The formation of liver fibrosis is mainly caused by the activation of hepatic stellate cells(HSCs)and the imbalance of extracellular matrix(ECM)production and degradation.The treatment of liver fibrosis mainly includes removing the cause,inhibiting the activation of HSCs,and inhibiting inflammation.NOD-like receptor(NLR)family,caspase activation and recruitment domain(CARD)domain containing 5/NOD27/CLR16.1(NLRC5)is a highly conserved member of the NLR family and is involved in inflammation and immune responses by regulating various signaling pathways such as nuclear factor-κB(NF-κB)signaling.It has been found that NLRC5 plays an important role in liver fibrosis,but its specific effect and possible mechanism remain to be fully elucidated.AIM To investigate the role of NLRC5 in the activation and reversion of HSCs induced with transforming growth factor-β(TGF-β)and MDI,and to explore its relationship with liver fibrosis.METHODS A total of 24 male C57BL/6 mice were randomly divided into three groups,including normal,fibrosis,and recovery groups.Twenty-four hours after a liver fibrosis and spontaneous reversion model was established,the mice were sacrificed and pathological examination of liver tissue was performed to observe the degree of liver fibrosis in each group.LX-2 cells were cultured in vitro and treated with TGF-β1 and MDI.Real-time quantitative PCR(qPCR)and Western blot were used to analyze the expression levels of NLRC5,α-smooth muscle actin(α-SMA),and collagen type I alpha1(Col1a1)in each group.The activity of NF-κB in each group of cells transfected with NLRC5-siRNA was detected.RESULTS Compared with the normal mice,the expression level of NLRC5 increased significantly(P<0.01)in the fibrosis group,but decreased significantly in the recovery group(P<0.01).In in vitro experiments,the content of NLRC5 was enhanced after TGF-β1 stimulation and decreased to a lower level when treated with MDI(P<0.01).The expression ofα-SMA and Col1a1 proteins and mRNAs in TGF-β1-mediated cells was suppr 展开更多
关键词 NLRC5 HEPATIC stellate cells Liver FIBROSIS Recovery
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Iron and liver fibrosis:Mechanistic and clinical aspects 预览
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作者 Kosha J Mehta Sebastien Je Farnaud Paul A Sharp 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第5期521-538,共18页
Liver fibrosis is characterised by excessive deposition of extracellular matrix that interrupts normal liver functionality.It is a pathological stage in several untreated chronic liver diseases such as the iron overlo... Liver fibrosis is characterised by excessive deposition of extracellular matrix that interrupts normal liver functionality.It is a pathological stage in several untreated chronic liver diseases such as the iron overload syndrome hereditary haemochromatosis,viral hepatitis,alcoholic liver disease,non-alcoholic fatty liver disease,non-alcoholic steatohepatitis and diabetes.Interestingly,regardless of the aetiology,iron-loading is frequently observed in chronic liver diseases.Excess iron can feed the Fenton reaction to generate unquenchable amounts of free radicals that cause grave cellular and tissue damage and thereby contribute to fibrosis.Moreover,excess iron can induce fibrosis-promoting signals in the parenchymal and non-parenchymal cells,which accelerate disease progression and exacerbate liver pathology.Fibrosis regression is achievable following treatment,but if untreated or unsuccessful,it can progress to the irreversible cirrhotic stage leading to organ failure and hepatocellular carcinoma,where resection or transplantation remain the only curative options.Therefore,understanding the role of iron in liver fibrosis is extremely essential as it can help in formulating iron-related diagnostic,prognostic and treatment strategies.These can be implemented in isolation or in combination with the current approaches to prepone detection,and halt or decelerate fibrosis progression before it reaches the irreparable stage.Thus,this review narrates the role of iron in liver fibrosis.It examines the underlying mechanisms by which excess iron can facilitate fibrotic responses.It describes the role of iron in various clinical pathologies and lastly,highlights the significance and potential of iron-related proteins in the diagnosis and therapeutics of liver fibrosis. 展开更多
关键词 IRON LIVER PATHOLOGIES LIVER FIBROSIS Hepatic stellate cells Cirrhosis
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慢性乙型肝炎合并肝脂肪变性患者的临床及病理特征分析
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作者 郑伟 潘宏义 +3 位作者 吴青青 尹乔乔 戴伊宁 潘红英 《中华临床感染病杂志》 CSCD 2019年第2期87-92,共6页
目的分析慢性乙型肝炎(Chronic hepatitis B,CHB)合并肝脂肪变性患者的临床特点,并对其病理特征进行分析。方法回顾性分析2015年9月至2018年9月在浙江省人民医院行肝穿刺检查的CHB患者共841例,根据肝脏病理组织中脂肪变性程度,分为脂肪... 目的分析慢性乙型肝炎(Chronic hepatitis B,CHB)合并肝脂肪变性患者的临床特点,并对其病理特征进行分析。方法回顾性分析2015年9月至2018年9月在浙江省人民医院行肝穿刺检查的CHB患者共841例,根据肝脏病理组织中脂肪变性程度,分为脂肪变性组和非脂肪变性组,采用倾向值匹配法按照性别相同、年龄相近(≤1岁)的原则进行配对,共获得135对CHB患者,采用秩和检验和多因素Logistic回归分析CHB患者合并肝脂肪变性的临床特征及其危险因素,采用Spearman秩相关分析肝脂肪变性的程度与HBV DNA水平和肝脏炎症及纤维化的关系。结果多因素Logistic回归分析显示,超重/肥胖(χ^2=3.947,OR=1.436,95%CI 1.005~2.051,P<0.05)及高脂血症(χ^2=4.277,OR=1.803,95%CI 1.031~3.151,P<0.05)为CHB患者发生肝脂肪变性的危险因素。肝脂肪变性程度与血清HBeAg、HBV DNA均无相关性(Z=-1.762,r=-0.011,P>0.05),但与肝脏炎症活动度分级及纤维化程度分期呈负相关(r=-0.146和-0.192,P<0.05)。结论超重/肥胖及高脂血症的CHB患者容易发生脂肪变性,肝脂肪变性并不会加重CHB患者肝脏炎症及纤维化程度。 展开更多
关键词 乙型肝炎 慢性 肝脂肪变性 肝脏炎症 纤维化 倾向值匹配法
Anluohuaxianwan Alleviates Carbon Tetrachloride-Induced Hepatic Fibrosis in Rats through Upregulation of Peroxisome Proliferator-Activated Receptor-Gamma and Downregulation of Nuclear Factor-Kappa B/IκBα Signaling Pathway
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作者 Lin Wang Wei Lu +4 位作者 Yu-Hua Gao Hai-Jiang Yan Fei Pei Xue-En Liu Hui Zhuang 《世界中医药杂志:英文版》 2019年第2期95-103,共9页
Objective:The aim of this study was to investigate the effects of traditional Chinese medicine Anluohuaxianwan(ALHXW)on peroxisome proliferator-activated receptor-gamma(PPARγ)and nuclear factor-kappa B(NF-κB)signali... Objective:The aim of this study was to investigate the effects of traditional Chinese medicine Anluohuaxianwan(ALHXW)on peroxisome proliferator-activated receptor-gamma(PPARγ)and nuclear factor-kappa B(NF-κB)signaling pathways using a rat model of carbon model groups were gavaged with saline for 6 weeks.Liver function was measured,and liver fibrosis and necroinflammation were assessed.Protein and messenger RNA expression levels of PPARγ,NF-κB,and Inhibitorαof NF-κB(IκBα)were analyzed by Western blot and reverse transcription–quantitative polymerase chain reaction.Results:ALHXW markedly alleviated liver injury compared with the model group,as indicated by the improvements in disease status,the morphology of liver and spleen,the liver and spleen indexes,and liver function.The extent of liver fibrosis was improved,hepatic stellate cell activation was inhibited,the expression of PPARγand IκBαwas significantly higher,and the expression of NF-κB was significantly lower in the treatment group as compared with the model group.Conclusions:ALHXW treatment can alleviate CCl4-induced liver fibrosis in rats, and the potential antifibrogenic mechanisms may occur through the upregulation of PPARγ expression and downregulation of NF-κB/IκBα signaling pathway. 展开更多
关键词 Anluohuaxianwan hepatic fibrosis mechanism nuclear factor-kappa B/IκBα PEROXISOME proliferator-activated receptor-gamma
Roles of hepatic stellate cells in acute liver failure:From the perspective of inflammation and fibrosis 预览
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作者 Juan Li Ying-Ren Zhao Zhen Tian 《世界肝病学杂志:英文版(电子版)》 2019年第5期412-420,共9页
Acute liver failure(ALF)usually results in hepatocellular dysfunction and coagulopathy and carries a high mortality rate.Hepatic stellate cells(HSCs)are famous for their role in liver fibrosis.Although some recent stu... Acute liver failure(ALF)usually results in hepatocellular dysfunction and coagulopathy and carries a high mortality rate.Hepatic stellate cells(HSCs)are famous for their role in liver fibrosis.Although some recent studies revealed that HSCs might participate in the pathogenesis of ALF,the accurate mechanism is still not fully understood.This review focuses on the recent advances in understanding the functions of HSCs in ALF and revealed both protective and promotive roles during the pathogenesis of ALF:HSC activation participates in the maintenance of cell attachment and the architecture of liver tissue via extracellular matrix production and assists liver regeneration by producing growth factors;and HSC inflammation plays a role in relaying inflammation signaling from sinusoids to parenchyma via secretion of inflammatory cytokines.A better understanding of roles of HSCs in the pathogenesis of ALF may lead to improvements and novel strategies for treating ALF patients. 展开更多
关键词 ACUTE liver FAILURE HEPATIC stellate cells INFLAMMATION FIBROSIS
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NLRP3炎性体在心肌病和心律失常中的作用及研究进展
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作者 李威 刘明鑫 唐艳红 《心脏杂志》 CAS 2019年第3期334-338,共5页
NLRP3炎性体是先天免疫反应的参与者,通过相关激活信号来触发炎症。NLRP3炎性体在心肌细胞和心脏成纤维细胞中表达,通过水解含半胱氨酸的天冬氨酸蛋白水解酶(caspase-1)前体生成caspase-1促使白介素(IL)-1β、IL-18的成熟和释放而导致... NLRP3炎性体是先天免疫反应的参与者,通过相关激活信号来触发炎症。NLRP3炎性体在心肌细胞和心脏成纤维细胞中表达,通过水解含半胱氨酸的天冬氨酸蛋白水解酶(caspase-1)前体生成caspase-1促使白介素(IL)-1β、IL-18的成熟和释放而导致细胞焦亡,其浸润影响到心肌病和心律失常等心血管疾病的发生发展过程。在这篇综述中,我们介绍了NLRP3炎性体在心肌病和心律失常中的作用及相关机制,对探索针对NLRP3炎症体在此类疾病中的治疗方案有重要意义。 展开更多
关键词 NLRP3炎性体 心肌病 心律失常 焦亡 纤维化
microRNA在心脏重塑中的研究进展 预览
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作者 陈阳 王婧 马丽杰 《基础医学与临床》 CSCD 2019年第4期589-593,共5页
microRNAs(miRNAs)通过对其靶基因的转录后调控参与胚胎发育、细胞增殖、分化及凋亡等过程,与心血管疾病的发生发展密切相关。心脏重塑目前缺乏有效的治疗靶点和有效的诊疗标志物,并且心脏重塑的机制研究还不够深入。目前发现miRNAs可... microRNAs(miRNAs)通过对其靶基因的转录后调控参与胚胎发育、细胞增殖、分化及凋亡等过程,与心血管疾病的发生发展密切相关。心脏重塑目前缺乏有效的治疗靶点和有效的诊疗标志物,并且心脏重塑的机制研究还不够深入。目前发现miRNAs可通过不同的分子机制调节心肌纤维化、心肌肥大以及能量代谢调节重塑。 展开更多
关键词 MICRORNAS 心脏重塑 纤维化 肥大
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纤维化因子及NADPH氧化酶在心理应激诱导小鼠的食管纤维化中的表达及意义
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作者 买买提·依斯热依力 吾布力卡斯木·吾拉木 +2 位作者 艾克拜尔·艾力 李义亮 克力木·阿不都热依木 《中国医师杂志》 CAS 2019年第5期672-676,共5页
目的研究胶原蛋白1(colla-1)、转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)及还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX-4)在慢性束缚应激(CRS)模型小鼠食管中的表达水平,探讨心理应激诱导食管纤维化的发生及氧化应激所产... 目的研究胶原蛋白1(colla-1)、转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)及还原型烟酰胺腺嘌呤二核苷酸磷酸氧化酶4(NOX-4)在慢性束缚应激(CRS)模型小鼠食管中的表达水平,探讨心理应激诱导食管纤维化的发生及氧化应激所产生的影响。方法 20只雄性SPF级昆明小鼠随机分2组,即CRS组和对照组。CRS组小鼠每天在自制式束缚器中限制活动2 h,其余时间两组小鼠在相同环境中自由饮水摄食,实验持续14 d。通过Masson染色在电子显微镜下观察食管组织纤维化改变。采用免疫组化、实时定量RT-PCR、ELISA法检测NOX-4在小鼠食管中的表达,并通过实时定量RT-PCR法检测食管组织中纤维化因子colla-1、TGF-β1及α-SMA mRNA的表达量。结果与对照组小鼠相比,CRS组小鼠体重增加量低于对照组[(8.75±1.69)g vs (12.69±3.16)g],差异有统计学意义(t=3.11,P<0.05)。Masson染色镜下观察可见:CRS组小鼠食管黏膜上皮细胞间明显的纤维化表现,而对照组未见明显异常;免疫组化结果显示:NOX-4阳性着色细胞在大部分标本中均可见到,CRS组小鼠食管组织中阳性着色较对照组染色深且丰富;ELISA法检测结果表明,CRS组小鼠血清中NOX-4表达量明显高于对照组(1.42±0.05 vs 0.49±0.08),差异有统计学意义(t=-27.32,P<0.01)。实时定量RT-PCR实验结果表明,CRS组纤维化标志物(colla-1,TGF-β1,α-SMA)及NOX-4 mRNA表达水平是对照组的(2.43±0.36、2.78±0.13、2.24±0.18、2.48±0.40)倍,差异有统计学意义(t=-11.19、-38.86、-19.90、-10.37,P<0.01)。结论 colla-1、TGF-β1、α-SMA参与心理应激诱导食管纤维化的形成,而氧化应激在食管纤维化产生中具有重要的作用。 展开更多
关键词 应激 心理学 约束 身体的 胶原Ⅰ型 转化生长因子β1 肌动蛋白类 NADPH氧化酶4 纤维化 食管疾病 小鼠
白刺果提取物花色苷通过抑制肝脏纤维化及炎症减轻非酒精性脂肪性肝病 预览
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作者 杨江霞 白建英 +3 位作者 李付平 张秀芬 徐昭娟 赵冬 《中国病理生理杂志》 CAS CSCD 北大核心 2019年第6期1130-1135,共6页
目的:探讨白刺果提取物花色苷对高脂饮食导致的小鼠非酒精性脂肪肝的治疗作用和潜在机制。方法:高脂饮食建立小鼠非酒精性脂肪肝模型,用花色苷进行干预后,测量小鼠肝脏重量,检测小鼠血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(... 目的:探讨白刺果提取物花色苷对高脂饮食导致的小鼠非酒精性脂肪肝的治疗作用和潜在机制。方法:高脂饮食建立小鼠非酒精性脂肪肝模型,用花色苷进行干预后,测量小鼠肝脏重量,检测小鼠血清中丙氨酸氨基转移酶(ALT)和天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)含量;肝脏组织经过HE染色、油红O染色及Masson染色,观察小鼠组织形态、脂滴沉积和胶原纤维增生的情况;Western blot检测肝脏组织中炎症因子肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)、IL-6及IL-10的水平;免疫组化检测肝脏组织中巨噬细胞、白细胞和单核细胞的浸润情况及肝脏组织趋化因子CCL7和单核细胞趋化蛋白1(MCP-1)的表达。结果:白刺果提取物花色苷能显著抑制由高脂饮食引起的小鼠肝重、ALT、AST、TG、TC及LDL-C的增高;肝脏切片染色显示花色苷能缓解由高脂饮食引起的肝脏纤维化及炎性细胞的浸润;花色苷能降低肝脏中炎症因子TNF-α、IL-1β、IL-6和IL-10及炎性趋化因子CCL7和MCP-1的水平。结论:白刺果提取物花色苷显著缓解由高脂饮食引起的非酒精性脂肪性肝病,其机制主要是通过抑制肝脏中的炎性因子和炎性细胞的浸润并降低炎性趋化因子来实现的。 展开更多
关键词 花色苷 非酒精性脂肪性肝病 炎症 纤维化
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栀子苷抗肾间质纤维化的作用机制研究 预览
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作者 孙源博 李桂芹 +4 位作者 孙丽欣 王云枫 关利新 朱敏 贠捷 《中国现代医学杂志》 CAS 2019年第11期8-13,共6页
目的通过转化生长因子-β1(TGF-β1)/Smad-2信号通路探讨栀子苷对肾间质纤维化的影响。方法将SD大鼠随机分为对照组、正常大鼠灌胃组、模型组、30 mg/kg及15 mg/kg栀子苷组,每组8只。复制大鼠单侧输尿管结扎(UUO)肾间质纤维化模型,栀子... 目的通过转化生长因子-β1(TGF-β1)/Smad-2信号通路探讨栀子苷对肾间质纤维化的影响。方法将SD大鼠随机分为对照组、正常大鼠灌胃组、模型组、30 mg/kg及15 mg/kg栀子苷组,每组8只。复制大鼠单侧输尿管结扎(UUO)肾间质纤维化模型,栀子苷组连续给予栀子苷14 d,对照组和模型组灌胃同体积的生理盐水;实验结束后,取大鼠结扎侧的肾脏,用HE染色观察肾间质纤维化病理情况;全自动生化仪检测血肌酐和尿素氮;ELISA检测大鼠血清中白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量,Western blotting检测TGF-β1和Smad-2蛋白含量。结果与模型组比较,30 mg/kg和15 mg/kg栀子苷组大鼠血清中炎症因子TNF-α和IL-1β含量均降低(P <0.05),且呈剂量依赖性降低,MDA含量降低(P <0.05),SOD活性升高(P <0.05),且随着栀子苷浓度的增加而升高。与对照组比较,模型组肾脏组织中TGF-β1和Smad-2蛋白的表达增加(P <0.05);给予30和15 mg/kg的栀子苷后大鼠肾脏组织中TGF-β1和Smad-2蛋白表达降低(P <0.05)。结论栀子苷具有抗肾间质纤维化作用,其机制可能与通过抑制TGF-β1/Smad信号通路,降低炎症反应及氧化应激损伤有关。 展开更多
关键词 肾病 纤维化 栀子苷 转化生长因子-Β1 Smad-2
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Persistent elevation of fibrosis biomarker cartilage oligomeric matrix protein following hepatitis C virus eradication 预览
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作者 Kristofer Andréasson Goran Jonsson +1 位作者 Roger Hesselstrand Hans Norrgren 《世界肝病学杂志:英文版(电子版)》 2019年第3期330-334,共5页
Serum levels of cartilage oligomeric matrix protein (COMP) has been presented as a biomarker of liver fibrosis in several cross-sectional studies. COMP is also an essential mediator in carcinoma development and has al... Serum levels of cartilage oligomeric matrix protein (COMP) has been presented as a biomarker of liver fibrosis in several cross-sectional studies. COMP is also an essential mediator in carcinoma development and has also been associated with hepatocellular carcinoma. We present a prospective analysis of this biomarker in 38 patients with chronic hepatitis C who were subject to eradication therapy with direct acting antivirals. We confirm previous studies associating COMP elevation with liver cirrhosis. We also show how viral levels are correlated with COMP at baseline. In our prospective analysis, we report that successful eradication of hepatitis C results in improvement in liver stiffness and laboratory liver function tests at 1 year follow-up. In contrast, median COMP-levels remain unchanged during the study period. We conclude that the biomarker potential of COMP in the prospective evaluation of liver diseases, remains to be elucidated. 展开更多
关键词 Hepatitis C Chronic CARTILAGE oligomeric matrix protein FIBROSIS
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巨噬细胞在子宫内膜异位症中作用的研究进展
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作者 李雪 张宗峰 《中华生殖与避孕杂志》 CAS CSCD 北大核心 2019年第4期325-328,共4页
子宫内膜异位症(endometriosis,EMS)是一种引起女性慢性盆腔疼痛和不孕的常见妇科良性疾病,其细胞浸润、增生及复发等均属恶性生物学行为。在EMS患者腹腔内存在巨噬细胞数量增加、活化类型改变,并伴有促炎因子、生长因子、血管生成等变... 子宫内膜异位症(endometriosis,EMS)是一种引起女性慢性盆腔疼痛和不孕的常见妇科良性疾病,其细胞浸润、增生及复发等均属恶性生物学行为。在EMS患者腹腔内存在巨噬细胞数量增加、活化类型改变,并伴有促炎因子、生长因子、血管生成等变化。随着研究的深入,腹腔内异常的免疫反应似乎对疾病发展十分重要。本文总结巨噬细胞新近研究进展,及其在EMS发病机制中的作用,有助于开发EMS新的治疗策略。 展开更多
关键词 子宫内膜异位症 巨噬细胞 炎性介质 纤维化 上皮-间充质转化
六味地黄丸通过调控NF-κB及TGF-β/Smad双信号通路减轻糖尿病肾病炎症及纤维化
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作者 陶鹏宇 张悦 《广州中医药大学学报》 CAS 2019年第2期245-250,共6页
【目的】基于核因子kappa B(NF-κB)及转化生长因子β(TGF-β)/Smad双信号通路,观察六味地黄丸对糖尿病肾病炎症及纤维化的影响,探讨其对肾保护作用机制。【方法】将30只SD大鼠随机分为正常组、模型组、中药组,每组10只。采用一次性腹... 【目的】基于核因子kappa B(NF-κB)及转化生长因子β(TGF-β)/Smad双信号通路,观察六味地黄丸对糖尿病肾病炎症及纤维化的影响,探讨其对肾保护作用机制。【方法】将30只SD大鼠随机分为正常组、模型组、中药组,每组10只。采用一次性腹腔注射链脲佐菌素法复制糖尿病大鼠模型。中药组给予大鼠六味地黄丸(剂量为6.75 g·kg-1·d-1)灌胃,正常组、模型组给予大鼠等体积生理盐水灌胃。给药12周后取材,检测各组大鼠空腹血糖(FBG)、尿素氮(BUN)、血肌酐(SCr)、24h尿蛋白量(24h-Pro),采用苏木素-伊红(HE)与Masson染色观察肾组织病理变化,蛋白免疫印迹(Western blot)法检测肾组织NF-κB、单核细胞趋化蛋白1(MCP-1)、TGF-β、alpha平滑肌抗体(α-SMA)、Smad2、Smad3、Smad7的表达。【结果】生化指标结果显示:与正常组比较,模型组大鼠FBG、BUN、SCr、24h-Pro升高(P<0.01);六味地黄丸治疗后上述指标均有所改善(P<0.05)。HE与Masson染色结果显示,模型组肾小球官腔变窄、系膜基质增生及炎症细胞浸润,而中药组有所改善。Western blot结果显示:与正常组比较,模型组的NF-κB、MCP-1、TGF-β、α-SMA、Smad2、Smad3表达均呈明显上调,而Smad7表达降低(P<0.01);中药组NF-κB、MCP-1、TGF-β、α-SMA、Smad2、Smad3表达下调,Smad7表达上调(P<0.05)。【结论】六味地黄丸具有肾脏保护作用,其机制可能与通过调控NF-κB及TGF-β/Smad双信号通路,从而减轻糖尿病肾病炎症损伤和纤维化有关。 展开更多
关键词 六味地黄丸 糖尿病肾病 炎症 纤维化 NF-ΚB信号通路 TGF-Β SMAD信号通路 疾病模型 动物 大鼠
肝卵圆细胞活化的微环境及其与肝纤维化关系的研究进展
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作者 廖渭 高毅 +1 位作者 彭青 张鹏 《中华肝胆外科杂志》 CAS CSCD 北大核心 2019年第5期386-389,共4页
肝纤维化是多种慢性肝脏疾病发展至肝硬化的必经阶段,严重威胁着我国人民健康。卵圆细胞位于肝脏汇管区,具有多向分化潜能,在肝纤维化中扮演重要角色。卵圆细胞具有促纤维化和抗纤维化的双重作用,其活化、分化和迁移等与肝纤维化关系密... 肝纤维化是多种慢性肝脏疾病发展至肝硬化的必经阶段,严重威胁着我国人民健康。卵圆细胞位于肝脏汇管区,具有多向分化潜能,在肝纤维化中扮演重要角色。卵圆细胞具有促纤维化和抗纤维化的双重作用,其活化、分化和迁移等与肝纤维化关系密切。深入研究卵圆细胞的生物学特性及微环境调控机制,将为肝纤维化的治疗提供新策略。本文就卵圆细胞活化的微环境及其与肝纤维化的关系进行综述。 展开更多
关键词 纤维化 细胞微环境 干细胞 肝星状细胞 卵圆细胞
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