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Verification of RFID System Usability in Silage Bale Life Cycle Management 预览
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作者 Katariina Penttila Antti Suokannas Ilpo P?l?nen 《农业科学与技术:A》 2019年第2期120-130,共11页
Digitalization has nowadays raised interest in variable applications of farming.Increase of knowledge level,by means of unique identification,automation and control,farmers gain relevant business profit.This research ... Digitalization has nowadays raised interest in variable applications of farming.Increase of knowledge level,by means of unique identification,automation and control,farmers gain relevant business profit.This research is focused on the utilization of passive radio frequency identification(RFID)technology in silage bale application,both manual and automated level.Challenges arise due to silage conservation,varying environmental and seasonal conditions,different identification environments and RFID operation principle.Further maximum communication signal strength is limited by telecommunication standard regulations(e.g.,ETSI).The applicability of RFID technology with different commercial passive transponders is measured manually in a silage bale of 160 cm in diameter,covering 360 degrees around the bale.In addition,automated field tests are conducted in a real environment,where the data collection system is appended to a tractor and RFID reader antenna in a baler.Manual measurements are conducted as identification distance(meters)and transponder population(number of tags),while automated measurements are based on the number of successfully identified silage bales.Based on the manual measurement results,the most suitable tags for the automated field measurements were chosen,and the applicability to silage bale identification was verified.Field tests showed 100%success,with 151/151 uniquely identified silage bales.Achieved results prove that passive RFID operates well enough in silage bale identification,further enabling the development of digitalization of silage bale life cycle. 展开更多
关键词 Digibale SILAGE BALE LIFE-CYCLE management radio frequency IDENTIFICATION DIGITALIZATION of SILAGE BALE unique IDENTIFICATION
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New few parameters differential evolution algorithm with application to structural identification
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作者 Rita Greco Ivo Vanzi 《交通运输工程学报(英文版)》 CSCD 2019年第1期1-14,共14页
Differential evolution algorithm(DEA)is a stochastic,population-based global optimization method.In this paper,we propose new schemes for both mutation and crossover operators in order to enhance the performances of t... Differential evolution algorithm(DEA)is a stochastic,population-based global optimization method.In this paper,we propose new schemes for both mutation and crossover operators in order to enhance the performances of the standard DEA.The advantage of these proposed operators is that they are'parameters-less',without a tuning phase of algorithm parameters that is often a disadvantage of DEA.Once the modified differential evolutions are presented,a large comparative analysis is performed with the aim to assess both correctness and efficiency of the proposed operators.Advantages of proposed DEA are used in an important task of modern structural engineering that is mechanical identification under external dynamic loads.This is because of the importance of using a“parametersless”algorithm in identification problems whose characteristics typically vary strongly case by case,needing of a continuous set up of the algorithm proposed.This important advantage of proposed optimizers,in front of other identification algorithms,is used to develop a computer code suitable for the automatic identification of a simple supported beam subject to an impact load,that has been tested both using numerical simulations and real standard tests dynamic.The results point out that this algorithm is an interesting candidate for standard applications in structural identification problems. 展开更多
关键词 DIFFERENTIAL evolution PARAMETRIC IDENTIFICATION STRUCTURAL IDENTIFICATION Optimization
Location and Trajectory Identification from Microblogs
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作者 Na Ta Guo-Liang Li +1 位作者 Jun Hu Jian-Hua Feng 《计算机科学技术学报:英文版》 SCIE EI CSCD 2019年第4期727-746,共20页
The rapid development of social networks has resulted in a proliferation of user-generated content(UGC),which can benefit many applications.In this paper,we study the problem of identifying a user's locations from... The rapid development of social networks has resulted in a proliferation of user-generated content(UGC),which can benefit many applications.In this paper,we study the problem of identifying a user's locations from microblogs,to facilitate effective location-based advertisement and recommendation.Since the location information in a microblog is incomplete,we cannot get an accurate location from a local microblog.As such,we propose a global location identification method,Glitter.Glitter combines multiple microblogs of a user and utilizes them to identify the user's locations.Glitter not only improves the quality of identifying a user's location but also supplements the location of a microblog so as to obtain an accurate location of a microblog.To facilitate location identification,Glitter organizes points of interest(POIs)into a tree structure where leaf nodes are POIs and non-leaf nodes are segments of POIs,e.g.,countries,cities,and streets.Using the tree structure,Glitter first extracts candidate locations from each microblog of a user which correspond to some tree nodes.Then Glitter aggregates these candidate locations and identifies top-κlocations of the user.Using the identified top-κuser locations,Glitter refines the candidate locations and computes top-κlocations of each microblog.To achieve high recall,we enable fuzzy matching between locations and microblogs.We propose an incremental algorithm to support dynamic updates of microblogs.We also study how to identify users'trajectories based on the extracted locations.We propose an effective algorithm to extract high-quality trajectories.Experimental results on real-world datasets show that our method achieves high quality and good performance,and scales well. 展开更多
关键词 LOCATION IDENTIFICATION microblog TRAJECTORY IDENTIFICATION
Study on the Identification of Original Plant and Crude Herbs in Paris daliensis H. Li et V.G. Souku and Paris dulongensis H. Li et S. Kuritap 预览
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作者 Risha WEIZE Zhongjie HUANG +3 位作者 Jixiu SHEN Xuexue LI Zhengming YANG Yuan LIU 《药用植物研究(英文版)》 2019年第2期54-57,共4页
[Objectives] Via systematic comparative study on Paris daliensis and Paris dulongensis, the research aimed to provide test data for their character and microscopic identification.[Methods] Original plants, characters,... [Objectives] Via systematic comparative study on Paris daliensis and Paris dulongensis, the research aimed to provide test data for their character and microscopic identification.[Methods] Original plants, characters, microscopic identification of cross section and powder of rhizome were used.[Results] Microscopic characteristics of cross section and powder of rhizome were basically consistent with identification results of literature, and parenchyma cell of P. dulongensis had brown-red mass.[Conclusions] The research could provide reference basis for identification of authenticity and establishment of quality standards for original plants and medicinal materials of P. daliensis and P. dulongensis, and their further research and development. 展开更多
关键词 PARIS daliensis PARIS dulongensis Character IDENTIFICATION MICROSCOPIC IDENTIFICATION
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Pharmacognosy Identification of Xiaohuangcao ( Dendrobium loddigesi Rolfe) 预览
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作者 Miao ZHANG Li LI +3 位作者 Jianbei TENG Hua ZHU Zhonghua DAI Long CHEN 《农业生物技术:英文版》 CAS 2019年第3期177-179,共3页
[Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-l... [Objectives]Pharmacognosy identification was performed on Xiaohuangcao ( Dendrobium loddigesi Rolfe).[Methods]The medicinal materials were identified through original plants,characters,microscopic structure and thin-layer identification characteristics.[Results]D.loddigesi has obvious plant morphology,characters,microscopic structure and thin-layer identification characteristics.The stem of Xiaohuangcao is slender and cylindrical,and the surface is golden yellow;and the fiber bundles outside the vascular bundles are crescent-shaped or semi-circular in the stem transection.For the powder,crystal fiber can be observed;the vascular bundles are embedded with siliceous block cells;and there are more starch grains.In the thin layer chromatography,petroleum ether-ethyl acetate-butanone-glacial acetic acid (8.5∶ 3.5∶ 1.5∶ 5 d) was used as a developing solvent,and 10% sulfuric acid ethanol solution was used as a color developing agent.[Conclusions]The research results provide reference for the application of the medicinal material and the formulation of its related quality standards. 展开更多
关键词 DENDROBIUM loddigesi Rolfe CHARACTER IDENTIFICATION MICROSCOPIC IDENTIFICATION THIN-LAYER chromatography
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Identification of Microplankton in South China Sea with Image-Matching Individual PCR 预览
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作者 LI Si YANG Guanpin +1 位作者 ZHU Baohua PAN Kehou 《中国海洋大学学报:英文版》 SCIE CAS CSCD 2019年第1期219-226,共8页
In this study,marine microplankton were identified by combining standard light microscopy with Sanger 18S rRNA gene sequencing.The image-matching individual PCR technique was applied to identify the image collectable ... In this study,marine microplankton were identified by combining standard light microscopy with Sanger 18S rRNA gene sequencing.The image-matching individual PCR technique was applied to identify the image collectable unicellular microplankton to genera.Instead of pure strain culture and morphological identification,microplankton individual cells were isolated and fixed with glutaraldehyde,frozen and stored for months.Finally,they were imaged under a microscope and molecularly identified via phylogenetic analysis of their 18S ribosomal RNA gene(18S rDNA).Microplankton cells were collected at 30 locations in South China Sea,and were assigned to 21 known and 4 unidentified genera(2 uncultured fungi and 2 uncultured stramenopiles)with phylogenetic analysis in parallel to the morphological identification. 展开更多
关键词 image-matching INDIVIDUAL PCR MICROPLANKTON 18S ribosomal RNA gene morphological IDENTIFICATION molecular IDENTIFICATION South China Sea
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Development and Modeling of Remotely Operated Scaled Multi-wheeled Combat Vehicle Using System Identification
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作者 A.N.Ouda Amr Mohamed +2 位作者 Moustafa EI-Gindy Haoxiang Lang Jing Ren 《国际自动化与计算杂志:英文版》 EI CSCD 2019年第3期261-273,共13页
This paper describes the development and modeling of a remotely operated scaled multi-wheeled combat vehicle(ROMWCV)using system identification methodology for heading angle tracking.The vehicle was developed at the v... This paper describes the development and modeling of a remotely operated scaled multi-wheeled combat vehicle(ROMWCV)using system identification methodology for heading angle tracking.The vehicle was developed at the vehicle dynamics and crash research(VDCR)Lab at the University of Ontario Institute of Technology(UOIT)to analyze the characteristics of the full-size model.For such vehicles,the development of controllers is considered the most crucial issue.In this paper,the ROMWCV is developed first.An experimental test was carried out to record and analyze the vehicle input/output signals in open loop system,which is considered a multi-input-single-output(MISO)system.Subsequently,a fuzzy logic controller(FLC)was developed for heading angle tracking.The experiments showed that it was feasible to represent the dynamic characteristics of the vehicle using the system identification technique.The estimation and validation results demonstrated that the obtained identified model was able to explain 88.44%of the output variation.In addition,the developed FLC showed a good heading angle tracking. 展开更多
关键词 Autonomous multi-wheeled VEHICLE system IDENTIFICATION all wheel STEERING fuzzy logic(FL) PARAMETRIC IDENTIFICATION
高寒草地土壤产漆酶真菌的筛选及分子鉴定 预览
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作者 李欣 芦光新 +2 位作者 姚世庭 党宁 王英成 《草地学报》 CAS CSCD 北大核心 2019年第1期211-218,共8页
以高寒草地土壤分离出的9株真菌菌株为研究对象,采用rDNA-ITS方法对筛选出的产漆酶菌株进行分子鉴定,根据菌株在愈创木酚、蒽酮、邻苯二酚、邻联甲苯胺、邻甲苯胺、α-萘酚、联苯胺、没食子酸为底物的选择性培养基上的生长情况、菌落大... 以高寒草地土壤分离出的9株真菌菌株为研究对象,采用rDNA-ITS方法对筛选出的产漆酶菌株进行分子鉴定,根据菌株在愈创木酚、蒽酮、邻苯二酚、邻联甲苯胺、邻甲苯胺、α-萘酚、联苯胺、没食子酸为底物的选择性培养基上的生长情况、菌落大小、漆酶催化氧化还原反应产生的变色圈直径大小及其颜色深浅程度,进行产漆酶真菌初筛,采用液体产酶发酵法选用ABTS为底物测定漆酶活力进行复筛。鉴定结果为菌株1.9为Uncultured fungus clone.,2.1a为Scytalidium,310b为Marasmius tricolor,2.1c为Saccharicola,2.3a为fungal sp,2.4d为Saccharicola.,10a为Marasmiusrotalis,3.7c为Alternaria.WB为Verticilliumlongisporum。筛选结果表明:除菌株3.7c以外,其余8株菌株在以愈创木酚、蒽酮、邻联甲苯胺、联苯胺为底物的培养基上基本都产生氧化带,在没食子酸底物培养基上均不产生氧化带;菌株1.9,2.1a,310b,2.4d,10a,2.1c,2.3a均具有产漆酶活力,其中菌株310b具有较强产漆酶活力。 展开更多
关键词 产漆酶真菌 筛选 氧化带 酶活 鉴定
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一株可提升豆酱风味乳酸菌的筛选、鉴定及应用 预览
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作者 周其洋 侯杰 《中国酿造》 CAS 北大核心 2019年第6期69-73,共5页
通过研究多菌种共同发酵豆酱,从豆酱产品中筛选理化指标优良、风味浓郁的特征性耐盐乳酸菌,并对该乳酸菌进行菌种鉴定及应用。结果表明,采用原位筛选法、平板复筛,得到一株可以显著抑制枯草芽孢杆菌(Bacillus subtilis)的乳酸菌,通过分... 通过研究多菌种共同发酵豆酱,从豆酱产品中筛选理化指标优良、风味浓郁的特征性耐盐乳酸菌,并对该乳酸菌进行菌种鉴定及应用。结果表明,采用原位筛选法、平板复筛,得到一株可以显著抑制枯草芽孢杆菌(Bacillus subtilis)的乳酸菌,通过分子生物学技术该菌株被鉴定为乳酸片球菌(Pediococcus acidilactici)。将筛选获得的乳酸片球菌(P. acidilactici)ZF559应用于豆酱发酵体系后,可以使其总酸含量降低7.7%,氨基酸态氮含量提升24%,使枯草芽孢杆菌(B. subtilis)的数量降低了94.55%,从而提升豆酱品质。 展开更多
关键词 豆酱 乳酸菌 筛选 鉴定 应用
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桑树桑黄与杨树桑黄的分子辨别 预览
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作者 宋吉玲 陆娜 +5 位作者 王伟科 袁卫东 李海波 程俊文 亢学平 闫静 《四川大学学报:自然科学版》 CAS CSCD 北大核心 2019年第4期765-770,共6页
为准确鉴别桑黄(Sanghuangporus)近缘种桑树桑黄(S.sanghuang)与杨树桑黄(S.vaninii)、暴马桑黄(S.baumii),本研究基于核糖体基因rDNA内转录间隔区(internal transcribed spacer,ITS)序列分析技术,对桑黄真菌进行了系统发育分析与近缘... 为准确鉴别桑黄(Sanghuangporus)近缘种桑树桑黄(S.sanghuang)与杨树桑黄(S.vaninii)、暴马桑黄(S.baumii),本研究基于核糖体基因rDNA内转录间隔区(internal transcribed spacer,ITS)序列分析技术,对桑黄真菌进行了系统发育分析与近缘种的分子辨别研究.结果表明,在NJ系统发育树上,23个桑树桑黄、11个杨树桑黄和6个暴马桑黄菌株各自以很高的Bootstrap支持率聚为了三个独立的分支,种间差异明显.依据桑树桑黄和杨树桑黄的rDNA ITS序列差异,设计两对引物Sv_U1/Sv_L和Sv_U2/Sv_L,均可特异性地扩增杨树桑黄478 bp和651 bp的ITS片段,而不扩增桑树桑黄的ITS片段,因而可用于两个桑黄近缘种的快速分子辨别.本研究为探讨桑黄孔菌属真菌的系统发育关系提供了参考,并为桑黄种间的准确辨别提供了一种有效的分子辅助手段. 展开更多
关键词 桑黄 鉴别 分类 内转录间隔区 特异性引物
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荸荠贮藏期间2株腐败菌鉴定及其特性 预览
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作者 张巧 商飞飞 +1 位作者 段振华 唐小闲 《江苏农业学报》 CSCD 北大核心 2019年第1期189-194,共6页
为确定引起荸荠腐烂的细菌种类及其主要生物学特性,从腐烂的荸荠中分离到2株细菌,经16S RNA基因序列结果分析,确定其分别为木糖氧化无色杆菌(Achromobacter xylosoxidans)和产气肠杆菌(Enterobacter aerogenes)。对其生物学特性进行研... 为确定引起荸荠腐烂的细菌种类及其主要生物学特性,从腐烂的荸荠中分离到2株细菌,经16S RNA基因序列结果分析,确定其分别为木糖氧化无色杆菌(Achromobacter xylosoxidans)和产气肠杆菌(Enterobacter aerogenes)。对其生物学特性进行研究后发现,2株腐败菌生长速度较快;产气肠杆菌的耐酸、耐温、耐盐性较强,培养液初始pH 3.5,培养温度45℃,或培养液中添加7.5%的NaCl,仍不能完全抑制其生长,而木糖氧化无色杆菌的耐酸、耐温、耐盐性则较差;此外,2株菌株对青霉素、红霉素、链霉素及氯霉素的抗性较强,最低抑制质量浓度均高于1 024μg/ml,对庆大霉素、万古霉素及四环素的最低抑制质量浓度均低于1 024μg/ml,且产气肠杆菌的耐受力强于木糖氧化无色杆菌。本研究结果为荸荠贮藏过程中的抗菌防腐提供了理论依据。 展开更多
关键词 荸荠 贮藏 腐败菌 鉴定
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酱香型窖醅中产酯细菌的分离及鉴定 预览
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作者 刘苑皓 赵兴秀 +3 位作者 舒梨 何义国 徐元文 张磊 《中国酿造》 CAS 北大核心 2019年第6期74-78,共5页
为了从酱香型窖醅中筛选产酯菌株,通过筛选培养基(透明圈法)进行初筛,用指示剂法测定发酵液的总酯含量,最终选出一株产酯较高的菌株LH2,其发酵液的总酯含量达到了2.2 g/L。通过形态学观察、生理生化试验和分子生物学技术鉴定出菌株LH2... 为了从酱香型窖醅中筛选产酯菌株,通过筛选培养基(透明圈法)进行初筛,用指示剂法测定发酵液的总酯含量,最终选出一株产酯较高的菌株LH2,其发酵液的总酯含量达到了2.2 g/L。通过形态学观察、生理生化试验和分子生物学技术鉴定出菌株LH2为枯草芽孢杆菌(Bacillus subtilis)。采用顶空固相微萃取-气质联用(HS-SPME-GC-MS)技术对菌株LH2发酵产物的香气物质进行分析,共鉴定出21种香气成分,主要是酯类(18.70%)、酸类(2.76%)、吡嗪类(9.71%)和醇类(12.95%),并在产酯能力较强的同时也产生四甲基吡嗪等有益身体健康的物质,增加了菌株LH2在健康白酒方向进一步研究的价值。 展开更多
关键词 窖醅 产酯细菌 分离 鉴定
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3株生防真菌的杀线虫活性及种类鉴定
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作者 陈秀菊 李惠霞 +2 位作者 徐志鹏 罗宁 韩尚明 《大豆科学》 CAS CSCD 北大核心 2019年第4期576-583,共8页
大豆胞囊线虫是影响大豆产量的重要线虫,为了获得更多防治该线虫的真菌资源,在室内测定了生防菌D1、D7和A1孢子悬浮液对卵的寄生作用和发酵液的杀线虫活性。结果表明:孢子悬浮液处理12 d后,3株生防菌对大豆胞囊线虫卵的寄生率分别为68.... 大豆胞囊线虫是影响大豆产量的重要线虫,为了获得更多防治该线虫的真菌资源,在室内测定了生防菌D1、D7和A1孢子悬浮液对卵的寄生作用和发酵液的杀线虫活性。结果表明:孢子悬浮液处理12 d后,3株生防菌对大豆胞囊线虫卵的寄生率分别为68.83%、81.33%和79.50%。发酵液处理14 d后, 3株生防菌1×发酵液对卵孵化的抑制率分别为70.00%、77.78%和68.89%。3株生防菌发酵液处理3 d后,对二龄幼虫的校正死亡率分别为90.01%、86.74%和80.03%。通过形态学和rDNA-ITS序列比对及系统发育树分析,将菌株D1、D7和A1分别鉴定为曲霉属(Aspergillus sp.)、烟曲霉(A.fumigatus)和寄生曲霉(A.parasiticus)。 展开更多
关键词 大豆胞囊线虫 生防真菌 杀线活性 鉴定
牛支原体新疆株的分离鉴定 预览
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作者 凌晨 郝成武 +3 位作者 何海 张飞 候凤 贺笋 《中国畜牧兽医》 CAS 北大核心 2019年第5期1466-1473,共8页
为调查新疆规模化奶牛场病牛死亡原因并确定病原,本研究无菌采集7份肺炎病死牛病变肺组织样,通过牛支原体液体培养基和固体培养基分离到1株支原体,采用形态学观察和生化试验鉴定该分离株,采用支原体特异性引物和牛支原体16SrRNA通用引... 为调查新疆规模化奶牛场病牛死亡原因并确定病原,本研究无菌采集7份肺炎病死牛病变肺组织样,通过牛支原体液体培养基和固体培养基分离到1株支原体,采用形态学观察和生化试验鉴定该分离株,采用支原体特异性引物和牛支原体16SrRNA通用引物扩增基因序列并测序,使用DNAStar软件将分离菌株测序结果与GenBank中的标准株序列进行同源性比对,采用Mega6.0软件中的邻接法(Neighbor-Joining,NJ)依据16SrRNA序列构建分离株系统进化树。结果显示,分离株菌落呈典型的“煎蛋样”,菌落中心凹陷深入培养基,周边菲薄而透明,经Dienes染液染色后,菌落中心呈深蓝色。该分离株不分解葡萄糖、尿素、不水解精氨酸,血细胞吸附试验和溶血试验均呈阴性,氯化三苯基四氮唑还原反应呈阳性,产生膜和斑。PCR反应扩增出大小为1911bp的牛支原体特异性目的片段;分离株16SrRNA基因序列与牛支原体标准株PG45的序列同源性为99.8%,与牛支原体地方株(MbNM2012、MbHB0801、MbHubei-1、MbNingxia-1、MbCQ-W70和Mb08M)的同源性为99.3%~99.7%。系统进化树显示,分离株16SrRNA基因与MbNingxia-1株和Mb08M株亲缘关系较近,处于同一分支。本研究结果证实了引起病牛死亡的病原为牛支原体,为新疆牛支原体病的防治提供了科学依据。 展开更多
关键词 牛支原体 分离 鉴定 16SrRNA
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起重机械检验中危险因素的识别与控制研究 预览
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作者 卢兵 《世界有色金属》 2019年第9期297-298,共2页
本文从起重机危险因素入手,分析并探讨起重机危险因素的识别与控制,提高起重机械检验质量和效率,降低工伤事故发生率,降低起重机械安全威胁。
关键词 起重机械 危险因素 识别 检验 控制
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一类分数阶神经网络的自适应H_∞同步 预览
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作者 袁晓琳 莫立坡 《智能系统学报》 CSCD 北大核心 2019年第2期239-245,共7页
针对一类不确定分数阶神经网络系统,研究了其自适应H_∞同步问题和参数辨识问题。提出了一个新的可以使闭环系统实现H_∞同步的自适应控制协议,利用鲁棒控制的方法和Gronwall-Bellma不等式证明了驱动系统和响应系统在该协议下可以实现同... 针对一类不确定分数阶神经网络系统,研究了其自适应H_∞同步问题和参数辨识问题。提出了一个新的可以使闭环系统实现H_∞同步的自适应控制协议,利用鲁棒控制的方法和Gronwall-Bellma不等式证明了驱动系统和响应系统在该协议下可以实现同步,同时满足H_∞性能。通过仿真实验,验证了所设计控制协议的有效性,并且说明了利用该控制协议可以对未知参数进行辨识。 展开更多
关键词 分数阶 神经网络 自适应 H_∞ 同步 未知参数 辨识 控制器
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Bayesian analysis of two methods MALDI-TOF-MS system and culture test in otomycosis infection
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作者 Sibanarayan Rath Saumya Ranjan Das Rabindra Nath Padhy 《世界耳鼻咽喉头颈外科杂志(英文)》 2019年第1期6-13,共8页
Objective:Identification of otomycotic fungi using matrix-assisted laser desorption ionization (MALDI) time of flight (TOF) mass spectroscopy (MS) and to quantify pervasive errors with Bayes rule;values of sensitivity... Objective:Identification of otomycotic fungi using matrix-assisted laser desorption ionization (MALDI) time of flight (TOF) mass spectroscopy (MS) and to quantify pervasive errors with Bayes rule;values of sensitivity and specificity of culture test and MALDI-TOF-MS method are quantified.Method:Fungi cultured ear discharge samples were identified with culture test and MALDITOF-MS system.Minimum inhibitory concentration (MIC) or MEC (minimum effective concentration) for 6 antifungals were determined by antifungal susceptibility testing in vitro.With Bayes rule,sensitivity and specificity of both MALDI-TOF MS and culture test methods were computed.Results:Cultures yielded 42 fungal isolates which were confirmed as species (specified against each species) belonging to 8 genera,A.niger 22,Candida sp.7,A.fumigatus 3,A.flavus 3,mixed Aspergillus sp.3,Mucor sp.2,Rhizopus sp.1,and Scopulariopsis sp.1;and MALDITOF-MS system also confirmed those isolates.In vitro antifungal susceptibility testing with terms of MIC 50 and MIC 90,isolates fungi were highly susceptible to 6 antifungals;and caspofungin was the most active antifungal.The high value of specificity 84.6%,suggested a limited loss of confidence on the culture test at the absence of an infection,in comparison to MALDI-TOF-MS.Sensitivity of the culture test was 87.5%;this high figure strongly approves the culture test to be the dependable method for the otomycosis diagnosis,when the infection is stable.Conclusion:Based on sensitivity and specificity together the culture test could be done in face of the gold-standardMALDI-TOF-MS system the estimating both methods. 展开更多
关键词 Otomycosis MALDI-TOF-MS system Identification at SPECIES LEVEL Sensitivity SPECIFICITY
生防菌HFW217的鉴定及其对棉花枯萎病的防治效果 预览
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作者 丁建朋 范瑛阁 姚永生 《新疆农业科学》 CAS CSCD 北大核心 2019年第3期498-508,共11页
【目的】研究生防菌HFW217的种类及其对棉花枯萎病的防治效果,为其生物防治提供材料。【方法】采用16SrDNA、rpoB基因序列和生理生化方法,对HFW217进行鉴定,通过温室和田间药效试验,测定HFW217对棉花枯萎病的防效。【结果】菌株HFW217... 【目的】研究生防菌HFW217的种类及其对棉花枯萎病的防治效果,为其生物防治提供材料。【方法】采用16SrDNA、rpoB基因序列和生理生化方法,对HFW217进行鉴定,通过温室和田间药效试验,测定HFW217对棉花枯萎病的防效。【结果】菌株HFW217为革兰氏阳性菌、呈短杆状、有芽孢,其菌体的形态呈短杆状,具有一定的耐盐碱性,16SrDNA和rpoB基因序列与解淀粉芽孢杆菌Bacillus amloliquefaciens具有较高的同源性,经鉴定该菌是解淀粉芽孢杆菌B.amloliquefaciens。温室和田间对棉花枯萎病防治效果分别达到77.62%和73.18%,防效明显高于多菌灵。【结论】菌株HFW217是解淀粉芽孢杆菌,该菌对棉花枯萎病的预防和治疗作用显著。 展开更多
关键词 棉花枯萎病 解淀粉芽孢杆菌 生防菌 鉴定 防治效果
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基于差分进化算法的超声波电机Hammerstein非线性建模 预览
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作者 黄文文 宋璐 史敬灼 《微电机》 北大核心 2019年第5期6-10,共5页
特殊的机电能量转换过程,使超声波电机具有明显的非线性运行特性。本文建立超声波电机的Hammerstein非线性模型,为电机控制系统的设计与分析提供基础。基于实验数据,通过拟合确定模型的静态非线性环节为Guass函数。采用差分进化算法,对... 特殊的机电能量转换过程,使超声波电机具有明显的非线性运行特性。本文建立超声波电机的Hammerstein非线性模型,为电机控制系统的设计与分析提供基础。基于实验数据,通过拟合确定模型的静态非线性环节为Guass函数。采用差分进化算法,对模型参数进行辨识。模型计算数据与实验数据的对比,表明模型精度较高,建模方法有效。 展开更多
关键词 超声波电机 HAMMERSTEIN模型 差分进化算法 辨识
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高产优质甘薯新品种‘榕薯153’选育及特性鉴定 预览
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作者 郭德章 《中国农学通报》 2019年第2期21-27,共7页
以早熟、高产、优质,综合性状优良的‘金山57’为母本,以高抗、高干材料‘B1’为父本经定向杂交、多点综合鉴定选育获得高产优质甘薯新品种‘榕薯153’。对‘榕薯153’的形态特征、农艺性状、品质性状、抗病性、生产力、适应性进行试验... 以早熟、高产、优质,综合性状优良的‘金山57’为母本,以高抗、高干材料‘B1’为父本经定向杂交、多点综合鉴定选育获得高产优质甘薯新品种‘榕薯153’。对‘榕薯153’的形态特征、农艺性状、品质性状、抗病性、生产力、适应性进行试验研究,结果表明‘,榕薯153’萌芽性中等,全生育期生长势稳定。2013—2014年福建省甘薯区域试验平均鲜薯产量41.39 t/hm2,比对照‘金山57’增产5.26%;平均干物率27.68%,比对照‘金山57’高1.43个百分点;平均出粉率17.72%,比‘金山57’高1.27个百分点;食味评分80.2分,比‘金山57’高0.2分;外观评分80.5分,比‘金山57’高0.5分;抗蔓割病,中抗薯瘟病,耐贮性好。该品种于2016年通过福建省作物品种审定委员会审定(审定编号:闽审薯2016003),适合作为鲜食或加工薯广地域栽培。 展开更多
关键词 甘薯' 榕薯153’ 高产 优质 选育 鉴定
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