Salt stress a ects the growth and development of plants, which results in a decrease in crop quality and yield. In this study, we used tomato seedlings treated with salt and trehalose as experimental materials and ana...Salt stress a ects the growth and development of plants, which results in a decrease in crop quality and yield. In this study, we used tomato seedlings treated with salt and trehalose as experimental materials and analyzed them using the technique for order preference by similarity to ideal solution analysis to select the optimal trehalose concentration for treatment. We also determined the contents of sugar and abscisic acid (ABA) and detected the expression of genes involved in the metabolism of sugar and ABA by quantitative real-time PCR. Results showed that the optimal trehalose concentration was 2 mmol/L for tomato seedlings under salt stress. Exogenous trehalose decreased the starch content and increased the soluble sugar con- tent by a ecting the expression of genes related to the metabolism of starch and soluble sugar. Exogenous trehalose altered the accumulation and distribution of sugar by inducing the upregulation of sugar transporter genes. Furthermore, trehalose increased the ABA content to induce salt stress response by regulating the expression of genes related to the synthesis and metabolism of ABA. In conclusion, trehalose can e ectively alleviate salt stress and enhance salt tolerance of tomato. These ndings provide a novel perspective and a better resource to investigate the salt tolerance mechanism and a new method for alleviating salt stress in tomato.展开更多
Various agricultural crop residues including corn stover,corn cob,and sorghum stalk with a moisture content of 75 wt% were subjected to a long pretreatment (12-60h) with supercritical CO2 (scCO2),at low temperature (5...Various agricultural crop residues including corn stover,corn cob,and sorghum stalk with a moisture content of 75 wt% were subjected to a long pretreatment (12-60h) with supercritical CO2 (scCO2),at low temperature (50-80℃) and a pressure of 17.5-25.0 MPa.The sugar yields from the enzymatic hydrolysis (EH) of the pretreated samples were as much as three- to fourfold greater than those afforded by the raw materials.However,when pretreatment was conducted within a short time (e.g.0.5 h),as previously reported in the literature,only a slight increase in the EH sugar yields was observed.The proposed scCO2 pretreatment mechanism demonstrated the role of moisture in the system.Wetting,softening,and swelling were observed to mainly affect the lignocellulose when a suitable amount of water was added.Fin ally,the samples were analysed by X-ray diffraction and scan ning electron microscopy,before and after pretreatment,to investigate the changes in the microscopic structure of the biomass.展开更多
[Objectives]This study was conducted to provide basic information for the utilization,quality control and deep processing of‘Xintai Tianhong’hawthorn fruit resources.[Methods]The contents of sugar and acid component...[Objectives]This study was conducted to provide basic information for the utilization,quality control and deep processing of‘Xintai Tianhong’hawthorn fruit resources.[Methods]The contents of sugar and acid components in‘Xintai Tianhong’hawthorn fruit were determined by high performance liquid chromatography(HPLC).[Results]‘Xintai Tianhong’hawthorn fruit contained such four kinds of sugar components as fructose,sorbitol,glucose and sucrose,and such six kinds of organic acid components as oxalic acid,tartaric acid,malic acid,acetic acid,citric acid and succinic acid.The fruit had the highest sucrose content,accounting for 70.53%of the total sugar content,followed by fructose,glucose and sorbitol.For the organic acid components,the content of succinic acid was the highest,accounting for 47.32%of the total acid content,followed by citric acid,malic acid,oxalic acid and tartaric acid,and acetic acid had the lowest content.[Conclusions]‘Xintai Tianhong’hawthorn should be a succinic acid-type hawthorn variety,which can be used as a parent in genetic research and breeding practice.展开更多
A field investigation was executed in sugarcane producing area of Zhanjiang,Guangdong Province with YT03-373 as the test variety to understand the possible impacts of seaweed extracts on phenology,yield and quality at...A field investigation was executed in sugarcane producing area of Zhanjiang,Guangdong Province with YT03-373 as the test variety to understand the possible impacts of seaweed extracts on phenology,yield and quality attributes of sugarcane. Foliar application of seaweed extracts were carried out on sugarcane at seedling and tillering stages. Agronomic traits,yield,sugar content,leaf drought resistance and pest resistance of sugarcane were analyzed for optimization of application time at proper phenological stages. The results that compared to the control,the application of seaweed extracts at different stages of sugarcane enhanced production of sugarcane with increase rate of 10. 83%-12. 87%,and improved theoretical sugar yield of sugarcane up to 1. 9 t/ha while enhancing water retention capacity and water content of sugarcane leaves that consequently led to wilting risk reduction by escaping drought and improved resilience to drought impact on sugarcane. Seaweed extracts also performed excellent in pest management of sugarcane,and they exerted different control effects on sugarcane borers,aphids and thrips. An increase in output of sugarcane was observed to be 15. 09% compared to control,while the net income was improved by up to 32. 64% in the seaweed extracts application treatment. Foliar application of seaweed extracts not only enhanced growth,cold resistance,pest resistance,output value but also improved economic returns,giving a positive affirmation for the recommendation of seaweed extract application.展开更多
A pot experiment was conducted during winter growing season of 2014 at Homs Agriculture Research Center, General Commission for Scientific Researches (GCSAR), Syria. A factorial experiment arranged according to comple...A pot experiment was conducted during winter growing season of 2014 at Homs Agriculture Research Center, General Commission for Scientific Researches (GCSAR), Syria. A factorial experiment arranged according to complete randomized block design with six replications was used. A combination of four levels of saline irrigation water (tap water, 2,000, 4,000 and 6,000 ppm), with three K levels (180, 360 and 540 ppm), was used to evaluate the effects of saline irrigation water and K enrichment on some growth attributes of two sugar beet varieties (Semper and Alligator). Results showed that all studied growth attributes, i.e., leaf area (LA), leaf number (LN), total dry matter (TDM) and net assimilation rate (NAR) were decreased under salinity stress conditions compared to the control, while K enrichment significantly increased some of the studied characters such as LA, TDM and NAR, but the differences in LN were apparent according to increase in K levels. The variety Semper surpassed significantly the variety Alligator in LA, TDM and NAR. Results also indicated a significant interaction between salinity×potassium enrichment, varieties×potassium enrichment and salinity ? varieties.展开更多
Sugars are important energy source and signaling molecule in plant,and sugar transporters such as SWEET(sugars will eventually be exported transporters)play important roles in plant growth and development as well as b...Sugars are important energy source and signaling molecule in plant,and sugar transporters such as SWEET(sugars will eventually be exported transporters)play important roles in plant growth and development as well as biotic and abiotic stresses.In this study,a gene named MdSWEET17 was isolated from apple(Malus×domestic).Expression analysis suggested that MdSWEET17 expressed in all tested tissues(root,stem,leaf,flower and fruit)and the transcript levels were different.Furthermore,MdSWEET17 transgenic tomatoes and wild type were treated with drought.The results showed transgenic lines had higher drought tolerance and accumulated more fructose.Taken together,these findings indicated that MdSWEET17 take part in drought stress response and the regulation of fructose.展开更多
In insects, the gustatory system plays a crucial role in multiple physiological behaviors, including feeding, toxin avoidance, courtship, mating and oviposition. Gustatory stimuli from the environment are recognized b...In insects, the gustatory system plays a crucial role in multiple physiological behaviors, including feeding, toxin avoidance, courtship, mating and oviposition. Gustatory stimuli from the environment are recognized by gustatory receptors. To date, little is known about the function of gustatory receptors in agricultural pest insects. In this study, we cloned a sugar gustatory receptor gene, N/Gr11, from the brown planthopper (BPH), Nilaparvata lugens (Stal), a serious pest of rice in Asia;we then identified its ligands, namely, fructose, galactose and arabinose, by calcium imaging assay. After injection of N/Gr11 doublestranded RNA, we found that the number of eggs laid by BPH decreased. Moreover, we found that N/Gr11 inhibited the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and promoted the phosphorylation of protein kinase B (AKT). These findings demonstrated that N/Gr11 could accelerate the fecundity of BPH through AMPK- and AKT-mediated signaling pathways. This is the first report to indicate that a gustatory receptor modulates the fecundity of insects and that the receptor could be a potential target for pest control.展开更多
Objective:Carbohydrates,proteins,and fats are energy sources needed by the body for performing daily activities and generating primary energy substances.In women who have undergone menopause,the function of thyroid ho...Objective:Carbohydrates,proteins,and fats are energy sources needed by the body for performing daily activities and generating primary energy substances.In women who have undergone menopause,the function of thyroid hormone in their body begins to decline and thus affects the ability of the body to produce energy.The purpose of this study was to determine the correlation between the intakes of carbohydrates,proteins,and fats and random blood sugar levels in menopausal women.Methods:This study was a correlational analytical research with a cross-sectional design,which was conducted in 72 menopausal women recruited by the purposive sampling technique.In this study,a 24-hour food recall form was used,and randomized blood sugar levels were measured using a glucometer.The analysis of the data was performed using a Pearson product moment and multiple linear regression.Results:Carbohydrates and fats together affected random blood sugar levels with an F-value of 25.810 and a p-value of 0.000.Meanwhile,adjusted R2 showed the value of 0.411,indicating that the difference in the intake of carbohydrates and fats together affected random blood sugar levels by 41.1%.Conclusions:Intake of carbohydrates and fats affected random blood sugar levels,whereas the rest were influenced by other factors,and protein intake was unrelated to random blood sugar levels in menopausal women.展开更多
This study evaluated the changes in sugar metabolism and fruit quality of diff erent pear cultivars during cold storage using seven major commercial pear cultivars belonging to diff erent Pyrus species, such as P. bre...This study evaluated the changes in sugar metabolism and fruit quality of diff erent pear cultivars during cold storage using seven major commercial pear cultivars belonging to diff erent Pyrus species, such as P. bretschneideri Rehd.("Huangguan,""Yali"), P. pyrifolia Nakai.("Wonhwang,""Hosui"), P. ussuriensis Maxim.("Jingbai,""Nanguo"), and P. communis L.("Bartlett"). The fi rmness, respiration rate, titratable acidity, total soluble solids, sugar content, and enzyme activity of the seven pear cultivars were investigated. SPSS was used for analyzing the signifi cance of diff erent indexes. Results showed that fructose was the dominant sugar, accounting for > 60% of total sugars, followed by glucose and sucrose. The respiration peak of almost all cultivars appeared within 60 days. The levels of fructose, glucose, sucrose, and total soluble solids increased within 90 days and then generally decreased. Acid invertase showed the highest activity among all pear cultivars, followed by neutral invertase, sucrose synthetase, and sucrose phosphate synthetase during storage.展开更多
基金This study was supported by the National Natural Science Foundation of China (Nos. 31671899 and 31871848)We are very grateful to Dr. Daqi Fu (China Agricultural University) for providing the tomato seeds.
文摘Salt stress a ects the growth and development of plants, which results in a decrease in crop quality and yield. In this study, we used tomato seedlings treated with salt and trehalose as experimental materials and analyzed them using the technique for order preference by similarity to ideal solution analysis to select the optimal trehalose concentration for treatment. We also determined the contents of sugar and abscisic acid (ABA) and detected the expression of genes involved in the metabolism of sugar and ABA by quantitative real-time PCR. Results showed that the optimal trehalose concentration was 2 mmol/L for tomato seedlings under salt stress. Exogenous trehalose decreased the starch content and increased the soluble sugar con- tent by a ecting the expression of genes related to the metabolism of starch and soluble sugar. Exogenous trehalose altered the accumulation and distribution of sugar by inducing the upregulation of sugar transporter genes. Furthermore, trehalose increased the ABA content to induce salt stress response by regulating the expression of genes related to the synthesis and metabolism of ABA. In conclusion, trehalose can e ectively alleviate salt stress and enhance salt tolerance of tomato. These ndings provide a novel perspective and a better resource to investigate the salt tolerance mechanism and a new method for alleviating salt stress in tomato.
基金the National Natural Science Foundation of China (21376045, 21506027)Chinese Postdoctoral Science Foundation (2015M571307)+1 种基金Petrochemicals Joint Fund between the National Natural Science Foundation of China, the China National Petroleum Corporation (U1662130)the Fundamental Research Funds for the Central Universities.
文摘Various agricultural crop residues including corn stover,corn cob,and sorghum stalk with a moisture content of 75 wt% were subjected to a long pretreatment (12-60h) with supercritical CO2 (scCO2),at low temperature (50-80℃) and a pressure of 17.5-25.0 MPa.The sugar yields from the enzymatic hydrolysis (EH) of the pretreated samples were as much as three- to fourfold greater than those afforded by the raw materials.However,when pretreatment was conducted within a short time (e.g.0.5 h),as previously reported in the literature,only a slight increase in the EH sugar yields was observed.The proposed scCO2 pretreatment mechanism demonstrated the role of moisture in the system.Wetting,softening,and swelling were observed to mainly affect the lignocellulose when a suitable amount of water was added.Fin ally,the samples were analysed by X-ray diffraction and scan ning electron microscopy,before and after pretreatment,to investigate the changes in the microscopic structure of the biomass.
基金Agricultural Improved Variety Project of Shandong Province(2016LZGC034).
文摘[Objectives]This study was conducted to provide basic information for the utilization,quality control and deep processing of‘Xintai Tianhong’hawthorn fruit resources.[Methods]The contents of sugar and acid components in‘Xintai Tianhong’hawthorn fruit were determined by high performance liquid chromatography(HPLC).[Results]‘Xintai Tianhong’hawthorn fruit contained such four kinds of sugar components as fructose,sorbitol,glucose and sucrose,and such six kinds of organic acid components as oxalic acid,tartaric acid,malic acid,acetic acid,citric acid and succinic acid.The fruit had the highest sucrose content,accounting for 70.53%of the total sugar content,followed by fructose,glucose and sorbitol.For the organic acid components,the content of succinic acid was the highest,accounting for 47.32%of the total acid content,followed by citric acid,malic acid,oxalic acid and tartaric acid,and acetic acid had the lowest content.[Conclusions]‘Xintai Tianhong’hawthorn should be a succinic acid-type hawthorn variety,which can be used as a parent in genetic research and breeding practice.
基金Supported by Guangdong Provincial Science and Technology Plan(2017A070701030,2017B030314123)Earmarked Fund for China Agriculture Research System(CARS-170203)+1 种基金Project of Guangdong Academy of Sciences(2017GDASCX-0105),2019GDASYL-0104012)Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences(1630052019001).
文摘A field investigation was executed in sugarcane producing area of Zhanjiang,Guangdong Province with YT03-373 as the test variety to understand the possible impacts of seaweed extracts on phenology,yield and quality attributes of sugarcane. Foliar application of seaweed extracts were carried out on sugarcane at seedling and tillering stages. Agronomic traits,yield,sugar content,leaf drought resistance and pest resistance of sugarcane were analyzed for optimization of application time at proper phenological stages. The results that compared to the control,the application of seaweed extracts at different stages of sugarcane enhanced production of sugarcane with increase rate of 10. 83%-12. 87%,and improved theoretical sugar yield of sugarcane up to 1. 9 t/ha while enhancing water retention capacity and water content of sugarcane leaves that consequently led to wilting risk reduction by escaping drought and improved resilience to drought impact on sugarcane. Seaweed extracts also performed excellent in pest management of sugarcane,and they exerted different control effects on sugarcane borers,aphids and thrips. An increase in output of sugarcane was observed to be 15. 09% compared to control,while the net income was improved by up to 32. 64% in the seaweed extracts application treatment. Foliar application of seaweed extracts not only enhanced growth,cold resistance,pest resistance,output value but also improved economic returns,giving a positive affirmation for the recommendation of seaweed extract application.
文摘A pot experiment was conducted during winter growing season of 2014 at Homs Agriculture Research Center, General Commission for Scientific Researches (GCSAR), Syria. A factorial experiment arranged according to complete randomized block design with six replications was used. A combination of four levels of saline irrigation water (tap water, 2,000, 4,000 and 6,000 ppm), with three K levels (180, 360 and 540 ppm), was used to evaluate the effects of saline irrigation water and K enrichment on some growth attributes of two sugar beet varieties (Semper and Alligator). Results showed that all studied growth attributes, i.e., leaf area (LA), leaf number (LN), total dry matter (TDM) and net assimilation rate (NAR) were decreased under salinity stress conditions compared to the control, while K enrichment significantly increased some of the studied characters such as LA, TDM and NAR, but the differences in LN were apparent according to increase in K levels. The variety Semper surpassed significantly the variety Alligator in LA, TDM and NAR. Results also indicated a significant interaction between salinity×potassium enrichment, varieties×potassium enrichment and salinity ? varieties.
基金supported by the National Natural Science Foundation of China (31471854, 31772288)the Natural Science Foundation of Shandong Province, China (ZR2017ZC0328).
文摘Sugars are important energy source and signaling molecule in plant,and sugar transporters such as SWEET(sugars will eventually be exported transporters)play important roles in plant growth and development as well as biotic and abiotic stresses.In this study,a gene named MdSWEET17 was isolated from apple(Malus×domestic).Expression analysis suggested that MdSWEET17 expressed in all tested tissues(root,stem,leaf,flower and fruit)and the transcript levels were different.Furthermore,MdSWEET17 transgenic tomatoes and wild type were treated with drought.The results showed transgenic lines had higher drought tolerance and accumulated more fructose.Taken together,these findings indicated that MdSWEET17 take part in drought stress response and the regulation of fructose.
基金the Foundation of Guangzhou Science and Technology Project (201504010021)the National Natural Science Foundation of China (U1401212)the China Postdoctoral Science Foundation (2017M612808).
文摘In insects, the gustatory system plays a crucial role in multiple physiological behaviors, including feeding, toxin avoidance, courtship, mating and oviposition. Gustatory stimuli from the environment are recognized by gustatory receptors. To date, little is known about the function of gustatory receptors in agricultural pest insects. In this study, we cloned a sugar gustatory receptor gene, N/Gr11, from the brown planthopper (BPH), Nilaparvata lugens (Stal), a serious pest of rice in Asia;we then identified its ligands, namely, fructose, galactose and arabinose, by calcium imaging assay. After injection of N/Gr11 doublestranded RNA, we found that the number of eggs laid by BPH decreased. Moreover, we found that N/Gr11 inhibited the phosphorylation of adenosine monophosphate-activated protein kinase (AMPK) and promoted the phosphorylation of protein kinase B (AKT). These findings demonstrated that N/Gr11 could accelerate the fecundity of BPH through AMPK- and AKT-mediated signaling pathways. This is the first report to indicate that a gustatory receptor modulates the fecundity of insects and that the receptor could be a potential target for pest control.
文摘Objective:Carbohydrates,proteins,and fats are energy sources needed by the body for performing daily activities and generating primary energy substances.In women who have undergone menopause,the function of thyroid hormone in their body begins to decline and thus affects the ability of the body to produce energy.The purpose of this study was to determine the correlation between the intakes of carbohydrates,proteins,and fats and random blood sugar levels in menopausal women.Methods:This study was a correlational analytical research with a cross-sectional design,which was conducted in 72 menopausal women recruited by the purposive sampling technique.In this study,a 24-hour food recall form was used,and randomized blood sugar levels were measured using a glucometer.The analysis of the data was performed using a Pearson product moment and multiple linear regression.Results:Carbohydrates and fats together affected random blood sugar levels with an F-value of 25.810 and a p-value of 0.000.Meanwhile,adjusted R2 showed the value of 0.411,indicating that the difference in the intake of carbohydrates and fats together affected random blood sugar levels by 41.1%.Conclusions:Intake of carbohydrates and fats affected random blood sugar levels,whereas the rest were influenced by other factors,and protein intake was unrelated to random blood sugar levels in menopausal women.
基金a key project in the National Science and Technology Pillar Program during the 11th 5-year plan period (No. 2006BAD22B01)the National Natural Science Foundation of China (No. 31470091)Innovation Team of Tianjin Forestry and Pomology Research System (No. ITTFPRS2018009).
文摘This study evaluated the changes in sugar metabolism and fruit quality of diff erent pear cultivars during cold storage using seven major commercial pear cultivars belonging to diff erent Pyrus species, such as P. bretschneideri Rehd.("Huangguan,""Yali"), P. pyrifolia Nakai.("Wonhwang,""Hosui"), P. ussuriensis Maxim.("Jingbai,""Nanguo"), and P. communis L.("Bartlett"). The fi rmness, respiration rate, titratable acidity, total soluble solids, sugar content, and enzyme activity of the seven pear cultivars were investigated. SPSS was used for analyzing the signifi cance of diff erent indexes. Results showed that fructose was the dominant sugar, accounting for > 60% of total sugars, followed by glucose and sucrose. The respiration peak of almost all cultivars appeared within 60 days. The levels of fructose, glucose, sucrose, and total soluble solids increased within 90 days and then generally decreased. Acid invertase showed the highest activity among all pear cultivars, followed by neutral invertase, sucrose synthetase, and sucrose phosphate synthetase during storage.