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Analysis of the autophagy gene expression profile of pancreatic cancer based on autophagy-related protein microtubule-associated protein 1A/1B-light chain 3 预览
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作者 Yan-Hui Yang Yu-Xiang Zhang +3 位作者 Yang Gui Jiang-Bo Liu Jun-Jun Sun Hua Fan 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第17期2086-2098,共13页
BACKGROUND Pancreatic cancer is a highly invasive malignant tumor. Expression levels of the autophagy-related protein microtubule-associated protein 1A/1B-light chain 3 (LC3) and perineural invasion (PNI) are closely ... BACKGROUND Pancreatic cancer is a highly invasive malignant tumor. Expression levels of the autophagy-related protein microtubule-associated protein 1A/1B-light chain 3 (LC3) and perineural invasion (PNI) are closely related to its occurrence and development. Our previous results showed that the high expression of LC3 was positively correlated with PNI in the patients with pancreatic cancer. In this study, we further searched for differential genes involved in autophagy of pancreatic cancer by gene expression profiling and analyzed their biological functions in pancreatic cancer, which provides a theoretical basis for elucidating the pathophysiological mechanism of autophagy in pancreatic cancer and PNI. AIM To identify differentially expressed genes involved in pancreatic cancer autophagy and explore the pathogenesis at the molecular level. METHODS Two sets of gene expression profiles of pancreatic cancer/normal tissue (GSE16515 and GSE15471) were collected from the Gene Expression Omnibus. Significance analysis of microarrays algorithm was used to screen differentially expressed genes related to pancreatic cancer. Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to analyze the functional enrichment of the differentially expressed genes. Protein interaction data containing only differentially expressed genes was downloaded from String database and screened. Module mining was carried out by Cytoscape software and ClusterOne plug-in. The interaction relationship between the modules was analyzed and the pivot nodes between the functional modules were determined according to the information of the functional modules and the data of reliable protein interaction network.RESULTS Based on the above two data sets of pancreatic tissue total gene expression, 6098 and 12928 differentially expressed genes were obtained by analysis of genes with higher phenotypic correlation. After extracting the intersection of the two differential gene sets, 4870 genes were determined 展开更多
关键词 Pancreatic cancer Autophagy-related PROTEIN microtubule-associated PROTEIN 1A/1B-light chain 3 Perineural invasion Gene Ontology ANALYSIS Kyoto ENCYCLOPEDIA of Genes and Genomes pathway ANALYSIS Ubiquitin C
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形态学检测在自噬研究中的应用 被引量:3
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作者 花芳 胡卓伟 《药学学报》 CAS CSCD 北大核心 2016年第1期39-44,共6页
自噬是降解损伤及丧失功能的细胞器或蛋白,维持细胞更新和稳态的关键细胞过程。自噬可分为三个相对独立的步骤:自噬泡起始形成阶段、自噬小体形成阶段以及成熟降解阶段,每个阶段都有其相对独特的形态学特征或分子标志物变化。本文综述... 自噬是降解损伤及丧失功能的细胞器或蛋白,维持细胞更新和稳态的关键细胞过程。自噬可分为三个相对独立的步骤:自噬泡起始形成阶段、自噬小体形成阶段以及成熟降解阶段,每个阶段都有其相对独特的形态学特征或分子标志物变化。本文综述了自噬研究中常用的形态学研究方法、结果阐释及这些过程中的关键注意事项。 展开更多
关键词 自噬 透射电子显微镜 免疫荧光 微管相关蛋白1A/1B轻链3 WD重复结构域磷酸肌醇相互作用蛋白1 Keima
自噬流的检测方法 被引量:8
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作者 吕晓希 胡卓伟 《药学学报》 CAS CSCD 北大核心 2016年第1期45-51,共7页
自噬是调节真核细胞生长、死亡和能量代谢的重要生物学机制。细胞自噬行使其生物学功能的前提是自噬流的活化。大量证据表明自噬流受损与多种慢性炎性疾病,特别是肿瘤、神经退行性疾病及组织纤维化的发病密切相关,目前对于自噬流的检测... 自噬是调节真核细胞生长、死亡和能量代谢的重要生物学机制。细胞自噬行使其生物学功能的前提是自噬流的活化。大量证据表明自噬流受损与多种慢性炎性疾病,特别是肿瘤、神经退行性疾病及组织纤维化的发病密切相关,目前对于自噬流的检测是自噬与其相关疾病研究领域的热点。由于自噬流是一个由多个步骤组成的动态过程,因此往往需要精细的实验才能准确判断自噬流状态。本文将介绍自噬流的基本概念并总结目前常规使用的自噬流检测方法。这些方法将有助于自噬生物学机制的研究,并为自噬相关药物筛选提供有效的解决方案。 展开更多
关键词 自噬流 流式细胞术 串联荧光 长寿命蛋白 微管结合蛋白1A/1B-轻链3
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