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紫草的蒙医传统奶炮制工艺及质量标准研究
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作者 苏日娜 牧丹 那生桑 《亚太传统医药》 2019年第2期64-67,共4页
目的:采用传统的蒙药炮制方法,对紫草药材进行奶炮制,通过HPLC等对其生品及其炮制品的有效成分进行含量测定,为紫草的奶炮制品的质量标准控制提供依据。方法:通过TLC进行理化鉴别,UV法测定其生品和炮制品的羟基萘醌总色素的含量,HPLC法... 目的:采用传统的蒙药炮制方法,对紫草药材进行奶炮制,通过HPLC等对其生品及其炮制品的有效成分进行含量测定,为紫草的奶炮制品的质量标准控制提供依据。方法:通过TLC进行理化鉴别,UV法测定其生品和炮制品的羟基萘醌总色素的含量,HPLC法测定其β,β’-二甲基丙烯酰阿卡宁的含量。结果:TLC结果表明,紫草的炮制品和对照药材的斑点一致,表明奶炮制后其总色素成分基本没有发生变化;UV和HPLC的结果表明,炮制品的羟基萘醌总色素(0.56%~2.5%)和β,β’-二甲基丙烯酰阿卡宁(0.020%~0.52%)的含量与生品比较均有不同程度下降。结论:本研究可为今后的蒙医药中紫草的奶炮制品的质量标准及药理实验提供实验依据。 展开更多
关键词 紫草 奶炮制 HPLC β β’-二甲基丙烯酰阿卡宁
跑台运动通过激活AMPK减少APP/PS1小鼠海马Aβ沉积 预览
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作者 闫清伟 赵娜 +2 位作者 夏杰 李百侠 崔海燕 《中国体育科技》 CSSCI 北大核心 2019年第4期71-80,共10页
目的:探讨AMPK介导的运动减少APP/PS1小鼠海马Aβ沉积的分子机制。方法:将3月龄C57BL/6品系的APP/PS1小鼠和野生小鼠各48只分别随机分为APP/PS1安静对照组(ADC,n=24)、APP/PS1运动组(ADE,n=24)、野生安静对照组(WTC,n=24)和野生运动组(W... 目的:探讨AMPK介导的运动减少APP/PS1小鼠海马Aβ沉积的分子机制。方法:将3月龄C57BL/6品系的APP/PS1小鼠和野生小鼠各48只分别随机分为APP/PS1安静对照组(ADC,n=24)、APP/PS1运动组(ADE,n=24)、野生安静对照组(WTC,n=24)和野生运动组(WTE,n=24)。ADE、WTE组进行12周跑台运动,同时ADC、WTC组置于安静跑台环境。采用ELISA实验测海马ATP、AMP含量,RT-qPCR实验测海马AMPK和BACE1mRNA水平,Western Blot实验测海马AMPK及其磷酸化(p-AMPK)蛋白表达、Sirt1、PPARγ、PGC1α、BACE1、Aβ等蛋白表达,免疫组化实验测海马SPs水平。结果:1) 12周跑台运动可提高APP/PS1小鼠海马, ATP、AMP含量,增加AMP/ATP比值(P<0.05);2)上调AMPK mRNA,上调AMPK蛋白表达及p-AMPK水平,上调Sirt1、PPARγ、PGC1α蛋白表达水平(P<0.05);3)下调BACE1 mRNA和蛋白表达水平(P<0.05),同时下调Aβ蛋白和SPs(P<0.01)。结论:12周跑台运动减少APP/PS1小鼠海马Aβ沉积的分子机制可能和运动激活AMPK,改善海马能量代谢,继而调节AMPK-BACE1通路有关。 展开更多
关键词 跑台运动 单磷酸腺苷活化蛋白激酶 海马 能量代谢 β-位淀粉样前体蛋白裂解酶1 Β-淀粉样蛋白
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Rho/ROCK信号通路在阿尔茨海默病中的研究进展 预览
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作者 裴海艳 万君 +1 位作者 崔洁 王翔宇 《海南医学》 CAS 2019年第9期1187-1189,共3页
阿尔茨海默病(Alzheimer's disease,AD)是导致痴呆最常见的病因,随着人口的老龄化,患AD的人数逐年攀升,目前尚无有效的治疗方法。AD的发病机制尚未完全明了,目前认为其是一种多因素参与的疾病,其病理特征主要是β淀粉样蛋白(β-amyl... 阿尔茨海默病(Alzheimer's disease,AD)是导致痴呆最常见的病因,随着人口的老龄化,患AD的人数逐年攀升,目前尚无有效的治疗方法。AD的发病机制尚未完全明了,目前认为其是一种多因素参与的疾病,其病理特征主要是β淀粉样蛋白(β-amyloid protein,Aβ)的沉积和神经纤维素的缠结(neurofibrillary tangles,NFT)。目前认为减少Aβ的产生能延缓或者预防AD的发展,而Rho/ROCK信号通路参与此病理过程,故Rho/ROCK抑制剂成为了AD治疗研究的新靶点。他汀类药物能抑制Rho/ROCK 信号通路,为AD的治疗提供了新的思路,现将就AD、Rho/ROCK信号通路及他汀类药物之间的关系展开综述。 展开更多
关键词 阿尔茨海默病 RHO/ROCK信号通路 AΒ 抑制剂 他汀类药物
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铜催化β,γ-不饱和腙与二硫/硒化物的硫/硒胺基化反应:合成硫/硒化吡唑啉类化合物
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作者 任培星 齐林 +6 位作者 方卓越 吴天舒 高雅蒙 沈松 宋金燕 王力竞 李玮 《有机化学》 SCIE CAS CSCD 北大核心 2019年第6期1776-1786,共11页
发展了一类β,γ-不饱和腙与二硫/硒化物在铜催化下进行的自由基历程的硫/硒胺基化反应,合成了一系列的硫/硒化吡唑啉类化合物.该反应操作简便且反应条件温和.
关键词 铜催化 β γ-不饱和腙 硫/硒胺基化 吡唑啉
胰岛素样生长因子-1对Aβ25~35诱导的SH-SY5Y细胞损伤的保护作用及机制 预览
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作者 崔春英 刘鹏飞 +3 位作者 侯训尧 申超 洪艳 刘雪平 《中国老年学杂志》 CAS 北大核心 2019年第10期2436-2440,共5页
目的 观察胰岛素样生长因子(IGF)-1对Aβ25~35诱导的人神经母细胞瘤SH-SY5Y细胞损伤的保护作用,并初步探讨其可能机制。方法 将SH-SY5Y细胞随机分为对照(Control)组,Aβ25~35处理(Aβ25~35)组,IGF-1治疗(Aβ25~35+IGF-1)组,Wortmannin... 目的 观察胰岛素样生长因子(IGF)-1对Aβ25~35诱导的人神经母细胞瘤SH-SY5Y细胞损伤的保护作用,并初步探讨其可能机制。方法 将SH-SY5Y细胞随机分为对照(Control)组,Aβ25~35处理(Aβ25~35)组,IGF-1治疗(Aβ25~35+IGF-1)组,Wortmannin抑制剂(Aβ25~35+IGF-1+Wort)组,单纯渥曼青霉素(Wortmannin)组。CCK-8法检测细胞活力,检测乳酸脱氧酶(LDH)漏出率,判断细胞损伤程度,荧光探针DCFH-DA法检测细胞内活性氧(ROS)含量的变化,试剂盒测定丙二醛(MAD)含量及超氧化物气化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性,流式细胞仪分析法测定细胞凋亡率,Western印迹法检测相关蛋白表达。结果 与Aβ25~35组相比,Aβ25~35+IGF-1组细胞活力明显升高,LDH漏出率明显下降(P<0.05)。IGF-1可明显减少Aβ25~35诱导SH-SY5Y细胞ROS及MDA生成,增加SOD及GSH-Px酶活性(P<0.05)。与Aβ25~35组相比,IGF-1治疗还能增加蛋白激酶B(Akt)及叉头框蛋白(Fox)O3a磷酸化水平,降低Puma蛋白表达水平及细胞凋亡率(P<0.05)。而加入磷脂酰肌醇-3激酶(PI3K)特异性抑制剂Wortmannin预处理,可阻断上述IGF-1的保护作用(P<0.05)。结论 IGF-1对Aβ25~35诱导的SH-SY5Y细胞损伤的保护作用可能与激活PI3K/Akt信号通路有关。 展开更多
关键词 胰岛素样生长因子-1 Β淀粉样蛋白 蛋白激酶B 氧化应激 凋亡
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糖尿病大鼠前额叶部位淀粉样蛋白水平与自噬水平变化的关系研究 预览
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作者 马楼艳 李洺 +2 位作者 翟佳佳 吕雅丽 成蕊宁 《成都医学院学报》 CAS 2019年第1期6-11,共6页
目的观察糖尿病大鼠前额叶部位淀粉样蛋白(β-amyloid,Aβ)表达与自噬水平变化的关系。方法90只雄性SD大鼠随机分为正常组(n=45)和糖尿病组(n=45)。建立STZ诱导的糖尿病大鼠模型,分别在成模第8、10、12周时,从两组随机取出15只大鼠行Mor... 目的观察糖尿病大鼠前额叶部位淀粉样蛋白(β-amyloid,Aβ)表达与自噬水平变化的关系。方法90只雄性SD大鼠随机分为正常组(n=45)和糖尿病组(n=45)。建立STZ诱导的糖尿病大鼠模型,分别在成模第8、10、12周时,从两组随机取出15只大鼠行Morris水迷宫测试大鼠的学习与记忆能力,电镜检测大鼠前额叶神经元细胞的超微结构,qRT-PCR检测大鼠前额叶APP、自噬相关蛋白Beclin-1、LC3Ⅰ、LC3ⅡmRNA的表达情况,Western blot和免疫组化检测大鼠前额叶Aβ、Beclin-1、LC3Ⅰ、LC3Ⅱ和p62蛋白的表达情况。结果与正常组大鼠相比,糖尿病组大鼠在成模第8周Morris水迷宫测试成绩下降,且随着糖尿病大鼠病程的延长,认知功能下降明显(P<0.05)。与正常组大鼠相比,糖尿病大鼠在成模第8周时前额叶神经元细胞的超微结构改变,线粒体肿胀,到第12周时神经元细胞核碎裂,染色质固缩凝集。第8周糖尿病大鼠前额叶神经元细胞Aβ沉积明显高于正常组大鼠(P<0.05),且随着糖尿病大鼠成模时间的延长,Aβ沉积增加明显,呈时间依赖性(P<0.05)。糖尿病大鼠在成模第8周时,前额叶神经元细胞Beclin-1蛋白及mRNA表达增加(P<0.05),LC3Ⅱ/Ⅰ蛋白及mRNA的比值升高(P<0.05),到第12周时这种差异最为明显。p62表达两组间差异无统计学意义(P>0.05)。第8~12周,随着时间延长,Aβ表达水平与Beclin-1、LC3Ⅱ/Ⅰ表达呈正相关(P<0.01)。结论糖尿病大鼠自成模第8周开始出现认知功能的下降,这与前额叶神经元细胞自噬水平改变、自噬流不通、Aβ清除障碍有关。 展开更多
关键词 糖尿病 认知障碍 前额叶 AΒ 自噬
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有氧运动改善AD模型中枢神经元线粒体通透性转换孔功能
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作者 牟连伟 李岩 +1 位作者 顾博雅 赵丽 《北京体育大学学报》 CSSCI 北大核心 2019年第1期138-146,共9页
目的:观察12周规律有氧运动对APP/PS1双转基因小鼠中枢神经元线粒体通透性转换孔(mitochondrial permeability transition pore,m PTP)功能的影响。方法:6月龄雄性APP/PS1小鼠(AD模型)及C57BL/6J小鼠随机分为AD安静组(AS)、AD运动组(AE... 目的:观察12周规律有氧运动对APP/PS1双转基因小鼠中枢神经元线粒体通透性转换孔(mitochondrial permeability transition pore,m PTP)功能的影响。方法:6月龄雄性APP/PS1小鼠(AD模型)及C57BL/6J小鼠随机分为AD安静组(AS)、AD运动组(AE)、正常安静组(CS)、正常运动组(CE)。CE、AE组进行12周规律跑台运动,5 d/周,60 min/d。前10 min运动速度12 m/min,后50 min运动速度15 m/min,跑台坡度为0°。免疫组织化学、免疫荧光染色及Dot blot检测Aβ低聚物及多聚体含量;荧光染色检测m PTP开放程度;Western blot检测Cyp D、ANT1、ANT2、VDAC-1、COXIV、ABAD蛋白的相对含量;比色法检测COXIV、ABAD蛋白酶活性。结果:1) 9月龄AD组额叶皮层、海马出现明显病理性Aβ沉积,低聚物Aβ显著增加(P<0. 05);运动显著降低额叶皮层、海马多聚体Aβ斑块直径和分布区域(P<0. 05)以及低聚物Aβ含量(P<0. 05)。2)运动显著降低AD组额叶皮层、海马m PTP开放程度(P<0. 05);降低正常对照组额叶、海马m PTP的开放程度(P<0. 05)。3)运动显著降低AD组额叶皮层、海马m PTP相关组成蛋白Cyp D、ANT1及海马的VDAC-1表达(P<0. 05),提高ANT2蛋白表达(P<0. 05);同时显著降低正常对照组额叶皮层、海马Cyp D蛋白表达(P<0. 01),提高额叶皮层ANT2蛋白表达(P<0. 05)。4)运动显著提高AD组海马与线粒体功能相关的COXIV蛋白表达及活性(P<0. 05),提高额叶COXIV活性;降低海马ABAD蛋白表达,提高其活性(P<0. 01);同时提高正常对照组额叶、海马COXIV蛋白表达及活性(P<0. 05)。结论:12周规律有氧运动训练可有效地抑制9月龄APP/PS1双转基因小鼠Aβos含量及多聚体Aβ沉积,减少Aβ与m PTP相关组成蛋白Cyp D、ANT1、ANT2、VDAC-1相互作用,上调线粒体氧化磷酸化相关蛋白酶ABAD、COXIV的活性,进而调控m PTP的开放程度,增强线粒体能量代谢功能,是有氧运动促进神经可塑性的分子机制之一。 展开更多
关键词 有氧运动 APP/PS1小鼠 AΒ m PTP
Increasing the Content of β Phase of Poly(9,9-dioctylfluorene) by Synergistically Controlling Solution Aggregation and Extending Film-forming Time
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作者 Ya-Di Liu Qiang Zhang +2 位作者 Xin-Hong Yu Jian-Gang Liu Yan-Chun Han 《高分子科学:英文版》 SCIE CAS CSCD 2019年第7期664-673,共10页
For poly(9,9-dioctylfluorene)(PFO),β phase (coplanar conformation with the intra-chain torsion angle of 165°) has a greater conjugation length and higher degree of order compared to those of α phase, which favo... For poly(9,9-dioctylfluorene)(PFO),β phase (coplanar conformation with the intra-chain torsion angle of 165°) has a greater conjugation length and higher degree of order compared to those of α phase, which favors charge carrier transport. However, the highest content of β phase obtained so far is 45%. We propose to increase the content of β phase by promoting the solution aggregation of PFO molecules and extending film-forming time. For this purpose, 1,8-diiodooctane (DIO) is added to PFO o-xylene solution, which enhances the interaction of PFO chains and improves the planarity of PFO backbone, resulting in the formation of ordered aggregation. The aggregates act as nucleation centers to promote the formation of β phase. The content of β phase increases with increasing DIO concentration and reaches a platform of 39% as DIO is more than 4 vol%. Furthermore, the film is kept in a sealed environment with oxylene atmosphere for 3 h, thus the PFO molecules have enough time to diffuse to the crystallization front and achieve disorder-order transition. As a result, the crystallinity of PFO is improved significantly and the content of β phase increases to 52%, reaching the highest value reported so far. 展开更多
关键词 β Phase Ordered AGGREGATION Film-forming TIME Disorder-order transition
More than anti-malarial agents:therapeutic potential of artemisinins in neurodegeneration 预览
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作者 Bing-Wen Lu Larry Baum +2 位作者 Kwok-Fai So Kin Chiu Li-Ke Xie 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1494-1498,共5页
Artemisinin,also called qinghaosu,is originally derived from the sweet wormwood plant(Artemisia annua),which is used in traditional Chinese medicine.Artemisinin and its derivatives(artemisinins)have been widely used f... Artemisinin,also called qinghaosu,is originally derived from the sweet wormwood plant(Artemisia annua),which is used in traditional Chinese medicine.Artemisinin and its derivatives(artemisinins)have been widely used for many years as anti-malarial agents,with few adverse side effects.Interestingly,evidence has recently shown that artemisinins might have a therapeutic value for several other diseases beyond malaria,including cancers,inflammatory diseases,and autoimmune disorders.Neurodegeneration is a challenging age-associated neurological disorder characterized by deterioration of neuronal structures as well as functions,whereas neuroinflammation has been considered to be an underlying factor in the development of various neurodegenerative disorders,including Alzheimer’s disease.Recently discovered properties of artemisinins suggested that they might be used to treat neurodegenerative disorders by decreasing oxidation,inflammation,and amyloid beta protein(Aβ).In this review,we will introduce artemisinins and highlight the possible mechanisms of their neuroprotective activities,suggesting that artemisinins might have therapeutic potential in neurodegenerative disorders. 展开更多
关键词 ARTEMISININ inflammation neuroinflammation NEURODEGENERATION Alzheimer’s DISEASE Parkinson’s DISEASE Aβ ANTI-OXIDATION neuroprotection neural regeneration
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AD淀粉样蛋白级联假说相关酶及作用 预览
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作者 姚娜 梁迷 华茜 《继续医学教育》 2019年第4期130-133,共4页
以淀粉样蛋白级联假说为研究基础,假定β淀粉样蛋白(amyloid β-protein , Aβ)在AD 的致病级联反应中处于核心位置,其产生和清除失衡是AD 发病的主要原因。围绕Aβ的产生和清除,介绍了与之相关的各种酶类和受体,包括分泌酶α-分泌酶、... 以淀粉样蛋白级联假说为研究基础,假定β淀粉样蛋白(amyloid β-protein , Aβ)在AD 的致病级联反应中处于核心位置,其产生和清除失衡是AD 发病的主要原因。围绕Aβ的产生和清除,介绍了与之相关的各种酶类和受体,包括分泌酶α-分泌酶、β-分泌酶(BACE1)、γ-分泌酶,降解酶肾胰岛素残基溶酶(neprilysin,NEP)、胰岛素降解酶(insulin-degrading enzyme,IDE),纤溶酶(plasmin),内皮素转化酶(endothelin converting enzyme,ECE)和受体低密度脂蛋白受体相关蛋白(low-density lipoprotein receptorrelated Protein,LRP)和高度糖基化终产物受体(receptor for advanced glycation end products,RAGE)。文章提出随着与Aβ产生、聚集和清除相关的基因不断被发现,这些基因参与的相关通路有可能被作为可能的治疗靶点。 展开更多
关键词 AD淀粉样蛋白酶 AΒ
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Dependence of mechanical properties on the microstructure characteristics of a near β titanium alloy Ti-7333
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作者 Ruifeng Dong Jinshan Li +2 位作者 Hongchao Kou Jiangkun Fan Bin Tang 《材料科学技术学报:英文版》 SCIE EI CAS CSCD 2019年第1期48-54,共7页
In this work, the microstructure and the corresponding tensile properties of the rolled Ti-7Mo-3Nb-3Cr-3Al(Ti-7333) alloy before and after the thermal treatments were investigated. The results show that a strong α-fi... In this work, the microstructure and the corresponding tensile properties of the rolled Ti-7Mo-3Nb-3Cr-3Al(Ti-7333) alloy before and after the thermal treatments were investigated. The results show that a strong α-fiber texture is developed in the rolled Ti-7333 alloy. The deformed matrix and the texture significantly induce the variant selection of β phase. The high strength of the rolled Ti-7333 alloy is attributed to the <110> texture parallel to the tensile direction and the dispersed α phase within the matrix. After the solution treatment followed by the aging treatment, the texture decreases and the microstructure consists of the equiaxed β grains, the spheroidal αp phase and various needle-like α variants. Eventually, the alloy could achieve an optimal combination with the strength of about 1450 MPa,the ductility of about 10.5% and a considerable shear strength of about 775 MPa. This balance can be ascribed to the performance of the spheroidal αp phase and various needle-like αs variants. The results indicate that the Ti-7333 alloy could be a promising candidate material for the high-strength fastener. 展开更多
关键词 NEAR β titanium alloy HOT-ROLLED Ti-7333 TEXTURE Thermal treatment α phase Mechanical properties
阿尔茨海默病患者血浆脂联素及Aβ水平测定的临床意义
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作者 杨玲玲 冯亚静 《微量元素与健康研究》 CAS 2019年第2期24-25,共2页
目的:探讨阿尔茨海默病(AD)患者血浆脂联素(APN)及Aβ沉积相关指标在诊疗过程中的临床意义。方法:选取2016年5月~2017年12月门诊及病房治疗的AD患者40例作为AD组,选取同期住院治疗无中枢神经系统疾病、无痴呆的非神经系统疾病患者30例... 目的:探讨阿尔茨海默病(AD)患者血浆脂联素(APN)及Aβ沉积相关指标在诊疗过程中的临床意义。方法:选取2016年5月~2017年12月门诊及病房治疗的AD患者40例作为AD组,选取同期住院治疗无中枢神经系统疾病、无痴呆的非神经系统疾病患者30例作为对照组。检测两组患者血清中血浆脂联素及Aβ1-40和Aβ1-42的含量。结果:AD患者组APN水平明显低于对照组(P <0.05),AD患者组血清中Aβ1-40及Aβ1-42含量明显高于对照组(P <0.05)。Pearson相关性分析提示,AD患者APN水平与Aβ含量呈负相关(P <0.05)。结论:动态检测AD患者体内血浆脂联素及Aβ水平变化在AD临床诊疗中有着重要的意义,有利于了解病情发展状况、治疗效果及预后判断。 展开更多
关键词 阿尔茨海默病(AD) 血浆脂联素(APN) AΒ
Phytochrome B and AGB1 Coordinately Regulate Photomorphogenesis by Antagonistically Modulating PIF3 Stability in Arabidopsis
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作者 Pengbo Xu Hongli Lian +6 位作者 Feng Xu Ting Zhang Sheng Wang Wenxiu Wang Shasha Du Jirong Huang Hong-Quan Yang 《分子植物:英文版》 SCIE CAS CSCD 2019年第2期229-247,共19页
Phytochrome B (phyB), the primary red light photoreceptor, promotes photomorphogenesis in Arabidopsis by interacting with the basic helix-loop-helix transcriptional factor PIF3 and inducing its phosphorylation and deg... Phytochrome B (phyB), the primary red light photoreceptor, promotes photomorphogenesis in Arabidopsis by interacting with the basic helix-loop-helix transcriptional factor PIF3 and inducing its phosphorylation and degradation. Heterotrimeric G proteins are known to regulate various developmental processes in plants and animals. In Arabidopsis, the G-protein β subunit AGB1 is known to repress photomorphogenesis. However, whether and how phyB and AGB1 coordinately regulate photomorphogenesis are largely unknown. Here we show that phyB physically interacts with AGB1 in a red light-dependent manner and that AGB1 interacts directly with PIF3. Moreover, we demonstrate that the AGB1-PIF3 interaction inhibits the association of PIF3 with phyB, leading to reduced phosphorylation and degradation of PIF3, whereas the phyB-AGB1 interaction represses the association of PIF3 with AGB1, resulting in enhaneed phosphorylation and degradation of PIF3. Our results suggest that phyB and AGB1 antagonistically regulate PIF3 stability by dynamically interacting with each other and PIF3. This dynamic mechanism may allow plants to balanee phyB and G-protein signaling to optimize photomorphogenesis. 展开更多
关键词 ARABIDOPSIS PHYTOCHROME B heterotrimeric G-PROTEIN β sub unit AGB1 PIF3 PHOTOMORPHOGENESIS protein STABILITY
NKX6.3 protects against gastric mucosal atrophy by downregulatingβ-amyloid production 预览
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作者 Jung Hwan Yoon Yeon Soo Lee +4 位作者 Olga Kim Hassan Ashktorab Duane T Smoot Suk Woo Nam Won Sang Park 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第3期330-345,共16页
BACKGROUND Atrophic gastritis is characterized by loss of appropriate glands and reduction in gastric secretory function due to chronic inflammatory processes in gastric mucosa.Moreover,atrophic gastritis is considere... BACKGROUND Atrophic gastritis is characterized by loss of appropriate glands and reduction in gastric secretory function due to chronic inflammatory processes in gastric mucosa.Moreover,atrophic gastritis is considered as a precancerous condition of gastric cancer.However,little is known about the molecular mechanism underlying gastric mucosal atrophy and its contribution to gastric carcinogenesis.Thus,we hypothesized that transcription factor NKX6.3 might be involved in maintaining gastric epithelial homeostasis by regulating amyloidβ(Aβ)production.AIM To determine whether NKX6.3 might protect against gastric mucosal atrophy by regulating Aβproduction.METHODS We identified NKX6.3 depletion induced cell death by cell count and Western blot assay.Production and mechanism of Aβoligomer were analyzed by enzymelinked immunosorbent assay,Western blot,immunoprecipitation,real-time quantitative polymerase chain reaction and immunofluorescence analysis.We further validated the correlation between expression of NKX6.3,Helicobacter pylori CagA,Aβoligomer,apolipoprotein E(ApoE),andβ-secretase 1(Bace1)in 55 gastric mucosae.RESULTS NKX6.3 depletion increased both adherent and floating cell populations in HFE-145 cells.Expression levels of cleaved caspase-3,-9,and poly ADP ribose polymerase were elevated in floating HFE-145shNKX6.3 cells.NKX6.3 depletion produced Aβpeptide oligomers,and increased expression of ApoE,amyloid precursor protein,Aβ,Bace1,low-density lipoprotein receptor,nicastrin,high mobility group box1,and receptor for advanced glycosylation end product proteins.In immunoprecipitation assay,γ-secretase complex was stably formed only in HFE-145shNKX6.3 cells.In gastric mucosae with atrophy,expression of Aβpeptide oligomer,ApoE,and Bace1 was detected and inversely correlated with NKX6.3 expression.Treatment with recombinant Aβ1-42 produced Aβoligomeric forms and decreased cell viability in HFE-145shNKX6.3 cells.Additionally,NKX6.3 depletion increased expression of inflammatory cytokines and cyclooxygena 展开更多
关键词 NKX6.3 GASTRIC MUCOSA ATROPHY AMYLOID β Gastrokine 1
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糖尿病与阿尔茨海默病的研究进展
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作者 徐珊珊 李俊发 赵丽 《神经疾病与精神卫生》 2019年第2期190-194,共5页
目前受阿尔茨海默病(AD)困扰的患者数量呈指数型增长,但AD的发病机制仍不明晰,现有治疗方法只是姑息治疗。有研究发现2型糖尿病患者患AD概率很高,且约四分之三的AD患者患2型糖尿病,提示糖尿病可能与AD存在一定关系。目前研究发现有许多... 目前受阿尔茨海默病(AD)困扰的患者数量呈指数型增长,但AD的发病机制仍不明晰,现有治疗方法只是姑息治疗。有研究发现2型糖尿病患者患AD概率很高,且约四分之三的AD患者患2型糖尿病,提示糖尿病可能与AD存在一定关系。目前研究发现有许多因素将糖尿病和AD联系在一起,如Aβ、tau蛋白、突触损伤、氧化应激、晚期糖基化终末产物(AGEs)的产生、免疫功能异常等。现着重介绍糖尿病通过上述联系引发AD的相关分子机制。 展开更多
关键词 糖尿病 阿尔茨海默病 AΒ TAU 蛋白 综述
Monopole effects, core excitations, and β decay in the A = 130 hole nuclei near 132Sn
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作者 王韩奎 李志宏 +4 位作者 袁岑溪 陈志强 王宁 秦伟 何翊绮 《中国物理C:英文版》 SCIE CAS CSCD 2019年第5期35-40,共6页
The proton and neutron cross-shell excitations across the Z = 50 shell are investigated in the southwest quadrant of 132Sn by large-scale shell-model calculations with extended pairing and multipole-multipole force. T... The proton and neutron cross-shell excitations across the Z = 50 shell are investigated in the southwest quadrant of 132Sn by large-scale shell-model calculations with extended pairing and multipole-multipole force. The model space allows proton(neutron) core excitations, and both the low-and high-energy states for 130 In are well described, as found by comparison with the experimental data. The monopole effects between the proton orbit and neutron orbit are studied as the new monopole correction that perfectly reproduces the first 1+ level in 130In. The energy interval of proton(neutron) core excitations in 130In lies in the range of 4.5-6.5(2.0-4.1) MeV, and the high energy yrast states are predicted as neutron core excitations. The decays are calculated among the A=130 nuclei of 130In, 130Sn and 130Cd. 展开更多
关键词 MONOPOLE EFFECTS core-excitations β DECAY HOLE NUCLEI
细胞自噬在阿尔茨海默症中作用的研究进展 预览
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作者 陈昱 朱伟 +1 位作者 邵国 贾小娥 《生理科学进展》 CAS 北大核心 2019年第1期63-66,共4页
阿尔茨海默症(AD)是一种神经退行性疾病,学习和认知障碍是其主要特征。细胞自噬存在于真核细胞中,异常蛋白质及受损细胞器通过自噬降解。近年来自噬在AD中作用和治疗等方面有了一系列新进展。本文对自噬及其相关的基因、分子、信号通路... 阿尔茨海默症(AD)是一种神经退行性疾病,学习和认知障碍是其主要特征。细胞自噬存在于真核细胞中,异常蛋白质及受损细胞器通过自噬降解。近年来自噬在AD中作用和治疗等方面有了一系列新进展。本文对自噬及其相关的基因、分子、信号通路、功能以及在AD早期阶段变化等方面进行论述,并对自噬在AD发病机制和临床治疗方面进行综述。 展开更多
关键词 自噬 阿尔茨海默症 AΒ TAU
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2型糖尿病与阿尔茨海默病相关性机制的研究进展 预览
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作者 刘敏 谭丽阳 +1 位作者 张军 唐伟 《中国老年学杂志》 CAS 北大核心 2019年第3期746-749,共4页
2型糖尿病(T2DM)是非胰岛素依赖的糖尿病(NIDDM),成人型糖尿病(DM),主要以β细胞功能障碍和胰岛素抵抗为特征[1]。根据疾病防控中心(CDC)的统计,美国2010年新诊断的DM患者达1 700万,是1990年的3倍。DM影响了2 900万美国居民,每年花费24... 2型糖尿病(T2DM)是非胰岛素依赖的糖尿病(NIDDM),成人型糖尿病(DM),主要以β细胞功能障碍和胰岛素抵抗为特征[1]。根据疾病防控中心(CDC)的统计,美国2010年新诊断的DM患者达1 700万,是1990年的3倍。DM影响了2 900万美国居民,每年花费245美元。T2DM与肥胖、慢性炎症,细胞毒性,急性期蛋白产生. 展开更多
关键词 2型糖尿病 阿尔茨海默病(AD) 胰岛素抵抗 AΒ TAU蛋白
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Effect of Quyu Chencuo Formula(去菀陈莝方)on Renal Fibrosis in Obstructive Nephropathy Rats
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作者 ZHU Rui DU Xing-guo +2 位作者 YANG Sheng-lan WU Yan-ran LIU Jian-guo 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第3期190-196,共7页
Objective: To observe the effect of Quyu Chencuo Formula(去菀陈莝方, QCF) on renal fibrosis in rats with obstructive nephropathy. Methods: Twenty-four rats were randomly divided into three groups, 4 for sham operation... Objective: To observe the effect of Quyu Chencuo Formula(去菀陈莝方, QCF) on renal fibrosis in rats with obstructive nephropathy. Methods: Twenty-four rats were randomly divided into three groups, 4 for sham operation as the control group, 10 for unilateral ureteral obstruction(UUO) model group, and the rest 10 for QCF treating UUO model group. All rats were sacrificed under 3% pentobarbital(50 mg/kg) anesthesia on the 14 th day after surgery, then the right kidney samples of rats were harvested for hematoxylin eosin(HE) staining and Masson staining to observe the renal pathological changes. Immunohistochemistry and Western blotting were used to examine the expression of transforming growth factor β1(TGF-β1), and real-time polymerase chain reaction(RT-PCR) was employed to examine the expressions of TGF-β1, α-smooth muscle actin(α-SMA) and E-cadherin mRNA. Results: HE and Masson staining showed that the renal interstitial of the rats in the control group had no significant fibrotic lesion;in the model group, there were obvious interstitial fibrosis;for the QCF group, there were epithelial cell necrosis, infiltration of lymphocytes and mononuclear cells, aggravated interstitial fibrosis in varied degrees, but the pathological changes were less in the QCF group than in the model group. The immunohistochemistry and Western blotting results showed that the TGF-β1 expression was increased significantly in the model group, while decreased significantly in the QCF group(P<0.05);RT-PCR showed that the mRNA expression of α-SMA and TGF-β1 increased significantly in the model group, while both were significantly decreased in the QCF group compared with the model group(P<0.05). The mRNA expression of E-cadherin was decreased significantly in the model group, and it was significantly increased in the QCF group as compared with the model group(P<0.05). Conclusion: QCF may improve renal fibrosis by regulating the expressions of TGF-β1, α-SMA and E-cadherin, and prevent the progress of kidney fibrosis. 展开更多
关键词 UNILATERAL ureteral LIGATION Quyu Chencuo FORMULA E-cadherin transforming growth factor β 1 α-smooth muscle ACTIN kidney FIBROSIS Chinese medicine
基于SPR技术的表皮生长因子受体与新疆紫草中4种活性成分的相互作用
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作者 王嫚 李书文 +1 位作者 董旭 申刚义 《中国实验方剂学杂志》 CSCD 北大核心 2018年第13期32-36,共5页
目的:以人表皮生长因子受体(EGFR)为靶标,从左旋紫草素,乙酰紫草素,β,β-二甲基丙烯酰紫草素,β-乙酰氧基异戊酰紫草素等新疆紫草所含的4种柰醌类活性成分中筛选潜在的EGFR抑制剂。方法:利用表面等离子体共振(SPR)技术,首先通过... 目的:以人表皮生长因子受体(EGFR)为靶标,从左旋紫草素,乙酰紫草素,β,β-二甲基丙烯酰紫草素,β-乙酰氧基异戊酰紫草素等新疆紫草所含的4种柰醌类活性成分中筛选潜在的EGFR抑制剂。方法:利用表面等离子体共振(SPR)技术,首先通过预吸附实验,优化固定的p H和浓度,选择最佳条件,构筑EGFR生物芯片。在此基础上,以p H 7.4,浓度10 mmol·L-1的磷酸缓冲溶液(含0.005%聚山梨酯-20)作为相互作用时的缓冲溶液,实时动态研究EGFR与4种单体的相互作用。通过软件计算动力学参数,基于结合动力学常数大小,筛选抑制剂。结果:选择p H 4.5,10 mmol·L-1的乙酸缓冲液、质量分数为10 mg·L-1的EGFR为其最佳的固定条件,最终固定量为250 RU。相互作用结果显示4种单体中,β,β-二甲基丙烯酰紫草素与EGFR有明显的结合作用,其他3种单体无明确响应。动力学参数计算结果显示,EGFR与β,β-二甲基丙烯酰紫草素相互作用的结合速率常数ka为1.27×104L·mol(-1)·s(-1),解离速率常数kd为2.92×10-2s-1,解离平衡常数KD为2.31×10-6mol·L-1,卡方值(Chi2)为3.25。KD〈1×10-5mol·L-1,表明他们有较强的亲和力。结论:β,β-二甲基丙烯酰紫草素可能为EGFR的一种抑制剂。 展开更多
关键词 表皮生长因子受体 新疆紫草 表面等离子体共振技术 β β-二甲基丙烯酰紫草素
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