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Development of P genome-specific SNPs and their application in tracing Agropyron cristatum introgressions in common wheat 预览
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作者 Huihui Ma Jinpeng Zhang +6 位作者 Jing Zhang Shenghui Zhou Haiming Han Weihua Liu Xinming Yang Xiuquan Li Lihui Li 《作物学报:英文版》 CAS CSCD 2019年第2期151-162,共12页
As an importantwild relative ofwheat,Agropyron cristatumhas been successfully used forwheat improvement.Currently,a few useful agronomic traits of A.cristatum,such as high grain number per spike and resistance to dise... As an importantwild relative ofwheat,Agropyron cristatumhas been successfully used forwheat improvement.Currently,a few useful agronomic traits of A.cristatum,such as high grain number per spike and resistance to diseases,have been transferred into common wheat.However,the effective detection of small A.cristatum segmental introgressions in common wheat is still difficult.The objective of this study was to identify A.cristatum-specific single nucleotide polymorphisms (SNPs) for the detection of small alien segments in wheat.The transcriptome sequences of A.cristatum were aligned against wheat coding DNA sequences (CDS) for SNP calling.As a result,we discovered a total of 167,613 putative SNPs specific to the P genome of A.cristatum compared with the common wheat genomes.Among 230 selected SNPs with functional annotations related to inflorescence development and stress resistance,68 were validated as P genome-specific SNPs inmultiple wheat backgrounds using Kompetitive Allele Specific PCR (KASP) assays.Among them,55 SNPs were assigned to six homoeologous groups of the P genome using wheat-A.cristatum addition lines,and 6P-specific SNP markers were further physically mapped on different segments of chromosome 6P in 6P translocation lines.The P genome-specific SNPs were also validated by Sanger sequencing and used to detect the P chromatin in wheat-A.cristatum cryptic introgression lines.Two SNP markers (Unigene20217-182 and Unigene20307-1420) were detected in two wheat-A.cristatum introgression lines that showed enhanced grain number per spike and high resistance to powdery mildew.Together,thedeveloped P genome-specific SNPmarkers will accelerate thedetection of large numbers of wheat-A.cristatum derivatives andwill be helpful formarker-assisted transfer of desirable traits from A.cristatum into adapted wheat cultivars in wheat breeding programs. 展开更多
关键词 KASP marker Single NUCLEOTIDE polymorphism AGROPYRON cristatum TRITICUM AESTIVUM
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Characterization of TaCOMT genes associated with stem lignin content in common wheat and development of a gene-specific marker 预览
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作者 FU Lu-ping XIAO Yong-gui +5 位作者 YAN Jun LIU Jin-dong WEN Wei-e ZHANG Yong XIA Xian-chun HE Zhong-hu 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第5期939-947,共9页
Stem lignin content(SLC) in common wheat(Triticum aestivum L.) contributes to lodging resistance. Caffeic acid 3-O-methyltransferase(COMT) is a key enzyme involved in lignin biosynthesis. Characterization of TaCOMT ge... Stem lignin content(SLC) in common wheat(Triticum aestivum L.) contributes to lodging resistance. Caffeic acid 3-O-methyltransferase(COMT) is a key enzyme involved in lignin biosynthesis. Characterization of TaCOMT genes and development of gene-specific markers could enable marker-assisted selection in wheat breeding. In the present study, the full-length genomic DNA(gDNA) sequences of TaCOMT genes located on chromosomes 3 A, 3 B, and 3 D were cloned by homologous cloning. Two allelic variants, TaCOMT-3 Ba and TaCOMT-3 Bb, were identified and differed by a 222-bp insertion/deletion(InDel) in the 3′-untranslated region(3′-UTR). A co-dominant gene-specific marker based on this InDel was developed and designated as Ta COMT-3 BM. A total of 157 wheat cultivars and advanced lines grown in four environments were used to validate the associations between allelic patterns and SLC. The SLC of cultivars with TaCOMT-3 Ba was significantly(P<0.01) higher than that of those with TaCOMT-3 Bb, and the marker TaCOMT-3 BM could be effectively used in wheat breeding. 展开更多
关键词 Triticum aestivum L. caffeic acid 3-O-methyltransferase STEM lignin content gene-specific MARKER LODGING resistance
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Seedling and adult plant resistance to leaf rust in 46 Chinese bread wheat landraces and 39 wheat lines with known Lr genes 预览
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作者 ZHANG Pei-pei Takele Weldu Gebrewahid +3 位作者 ZHOU Yue LI Qing-luo LI Zai-feng LIU Da-qun 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第5期1014-1023,共10页
Wheat leaf rust,caused by Puccinia triticina(Pt),is an important foliar disease that has an important influence on wheat yield.The most economic,safe and effective way to control the disease is growing resistant culti... Wheat leaf rust,caused by Puccinia triticina(Pt),is an important foliar disease that has an important influence on wheat yield.The most economic,safe and effective way to control the disease is growing resistant cultivars.In the present study,a total of 46 wheat landraces and 34 wheat lines with known Lr(leaf rust resistance)genes were inoculated with 16Pt pathotypes for postulating seedling resistance gene(s)in the greenhouse.These cultivars and five wheat differential lines with adult plant resistance(APR)genes(Lr12,Lr22b,Lr34,Lr35 and Lr37)were also evaluated for identification of slow rusting resistance in the field trials in Baoding,Hebei Province of China in the 2014–2015 and 2015–2016 cropping seasons.Furthermore,10 functional molecular markers closely linked to 10 known Lr genes were used to detect all the wheat genotypes.Results showed that most of the landraces were susceptible to most of the Pt pathotypes at seedling stage.Nonetheless,Lr1 was detected only in Hongtangliangmai.The field experimental test of the two environments showed that 38 landraces showed slow rusting resistance.Seven cultivars possessed Lr34 but none of the landraces contained Lr37 and Lr46.Lr genes namely,Lr9,Lr19,Lr24,Lr28,Lr29,Lr47,Lr51 and Lr53 were effective at the whole plant stage.Lr18,Lr36 and Lr45 had lost resistance to part of pathotypes at the seedling stage but showed high resistance at the adult plant stage.Lr34 as a slowing rusting gene showed good resistance in the field.Four race-specific APR genes Lr12,Lr13,Lr35 and Lr37 conferred good resistance in the field experiments.Seven race-specific genes,Lr2b,Lr2c,Lr11,Lr16,Lr26,Lr33 and LrB had lost resistance.The 38 landraces showed slow rusting resistance to wheat leaf rust can be used as resistance resources for wheat resistance breeding in China. 展开更多
关键词 TRITICUM AESTIVUM L. WHEAT leaf rust gene postulation adult plant resistance
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有机肥替代化肥对小麦产量及土壤养分和酶活性的影响 预览
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作者 沈冰涛 张孝倩 +4 位作者 陈红 江旭聪 李孝良 汪建飞 肖新 《长江大学学报:自然科学版》 CAS 2019年第5期46-52,I0005,I0006共9页
为探讨有机肥替代化肥对麦田土壤肥力特征的影响,以淮北平原农田砂姜黑土为研究对象,采取大田试验,设置不施肥(CK)、100%化肥(UF)、20%有机肥+80%化肥(OF20)、40%有机肥+60%化肥(OF40)和100%有机肥(OF100)处理,分析有机肥替代化肥处理... 为探讨有机肥替代化肥对麦田土壤肥力特征的影响,以淮北平原农田砂姜黑土为研究对象,采取大田试验,设置不施肥(CK)、100%化肥(UF)、20%有机肥+80%化肥(OF20)、40%有机肥+60%化肥(OF40)和100%有机肥(OF100)处理,分析有机肥替代化肥处理对小麦(Triticum aestivum L.)产量及土壤养分和酶活性的影响。结果表明,有机肥替代20%化肥与单施化肥处理相比,产量提高1.86%,有机质、碱解氮、速效磷、速效钾含量及过氧化氢酶、蔗糖酶、磷酸酶活性,分别提高4.61%、6.39%、2.31%、2.20%、3.12%、2.44%、1.17%。有机肥替代40%化肥与单施化肥相比,有机质、速效磷、速效钾含量及过氧化氢酶、磷酸酶、脲酶活性分别提高4.03%、0.46%、1.46%、3.64%、0.43%、8.41%。由此表明,有机肥替代20%化肥可以较好提高小麦产量及土壤养分和部分酶活性。 展开更多
关键词 有机肥替代化肥 土壤养分 土壤酶活性 小麦(Triticum AESTIVUM L.)
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Transcriptome analysis of salt-stress response in three seedling tissues of common wheat 预览
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作者 Qiaoling Luo Wan Teng +5 位作者 Shuang Fang Hongwei Li Bin Li Jinfang Chu Zhensheng Li Qi Zheng 《作物学报:英文版》 CAS CSCD 2019年第3期378-392,共15页
Xiaoyan 60(XY60) is a new wheat variety bred in the laboratory of Zhensheng Li.After salt treatment, seedlings of XY60 maintain green leaves and produce longer roots than the high yielding cultivar Zhongmai 175(ZM175)... Xiaoyan 60(XY60) is a new wheat variety bred in the laboratory of Zhensheng Li.After salt treatment, seedlings of XY60 maintain green leaves and produce longer roots than the high yielding cultivar Zhongmai 175(ZM175).To explain these different phenotypes we carried out an RNA-Seq analysis using 12 samples from three tissues of both varieties subjected to salt and control treatments.By comparing data from the salt treated plants with the control, 703, 979, and 1197 differentially expressed genes(DEGs) were detected in new leaves, old leaves, and roots of XY60, respectively.The corresponding numbers for ZM175 were 613, 1401, and 1301.The most significantly enriched Gene Ontology(GO) terms and KEGG pathways were associated with polyunsaturated fatty acid(PUFA) metabolism in both new and old leaves from XY60.They were associated with photosynthesis and energy metabolism in ZM175.The most significantly enriched KEGG pathway in roots of both varieties was “glucosinolate biosynthesis”.In addition, jasmonic acid(JA) concentration in XY60 was higher than in ZM175, although it increased significantly in both varieties following salt treatment.Trends in relative expression levels of AOS, MYC2, and JAZ revealed by qRT-PCR were concordant with those from RNA-Seq.Our results suggest that PUFAs may contribute to salt tolerance in common wheat by enhancing the photosynthetic system and JA-related pathways. 展开更多
关键词 Jasmonic acid PHOTOSYNTHESIS RNA-Seq Salt tolerance TRITICUM AESTIVUM
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Reactive oxygen species are involved in cell death in wheat roots against powdery mildew 预览
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作者 LI Cheng-yang ZHANG Nan +2 位作者 GUAN Bin ZHOU Zhu-qing MEI Fang-zhu 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第9期1961-1970,共10页
Inoculation of wheat(Triticum aestivum L.)leaves with wheat powdery mildew fungus(Blumeria graminis f.sp.tritici)induces the cell death in adventitious roots.Reactive oxygen species(ROS)play a key role in respond to b... Inoculation of wheat(Triticum aestivum L.)leaves with wheat powdery mildew fungus(Blumeria graminis f.sp.tritici)induces the cell death in adventitious roots.Reactive oxygen species(ROS)play a key role in respond to biotic stress in plants.To study the involvement of ROS and the degree of cell death in the wheat roots following inoculation,ROS levels and microstructure of root cells were analyzed in two wheat cultivars that are susceptible(Huamai 8)and resistant(Shenmai 8)to powdery mildew fungus.At 18 d after powdery mildew fungus inoculation,only Huamai 8 displayed the leaf lesions,while root cell death occurred in both varieties.Huamai 8 had a high level of ROS accumulation,which is associated with increased root cell degradation,while in Shenmai 8,there was little ROS accumulation correlating with slight root cell degradation.The molecular study about the expression levels of ROS scavenging genes(MnSOD and CAT)in wheat roots showed that these genes expression decreased after the leaves of wheat was inoculated.The difference between Huamai 8 and Shenmai 8 on subcellular localization of H2 O2 and O2^–·was corresponded with the different down-regulation of the genes encoding for superoxide dismutase and catalase in two wheat cultivars.These results suggested that ROS were involved in the process by which powdery mildew fungus induced cell death in wheat roots. 展开更多
关键词 powdery MILDEW wheat(Triticum AESTIVUM L.) reactive oxygen species ULTRASTRUCTURE programmed cell death
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Dissecting conserved cis-regulatory modules of Glu-1 promoters which confer the highly active endosperm-specific expression via stable wheat transformation 预览
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作者 Jihu Li Ke Wang +7 位作者 Genying Li Yulian Li Yong Zhang Zhiyong Liu Xingguo Ye Xianchun Xia Zhonghu He Shuanghe Cao 《作物学报:英文版》 CAS CSCD 2019年第1期8-18,共11页
Wheat high-molecular-weight glutenin subunits(HMW-GS)determine dough elasticity and play an essential role in processing quality.HMW-GS are encoded by Glu-1 genes and controlled primarily at transcriptional level,impl... Wheat high-molecular-weight glutenin subunits(HMW-GS)determine dough elasticity and play an essential role in processing quality.HMW-GS are encoded by Glu-1 genes and controlled primarily at transcriptional level,implemented through the interactions between cis-acting elements and trans-acting factors.However,transcriptional mechanismof Glu-1 genes remains elusive.Here we made a comprehensive analysis of cis-regulatory elements within 1-kb upstream of the Glu-1 start codon(-1000 to-1)and identified 30 conserved motifs.Based on motif distribution pattern,three conserved cis-regulatory modules(CCRMs),CCRM1(-300 to-101),CCRM2(-650 to-400),and CCRM3(-950 to-750),were defined,and their functions were characterized in wheat stable transgenic lines transformed with progressive 5′deletion promoter::GUS fusion constructs.GUS staining,qPCR and enzyme activity assays indicated that CCRM2 and CCRM3 could enhance the expression level of Glu-1,whereas the 300-bp promoter(-300 to-1),spanning CCRM1 and core region(-100 to-1),was enough to ensure accurate Glu-1 initiation at 7 days after flowering(DAF)and shape its spatiotemporal expression pattern during seed development.Further transgenic assays demonstrated that CCRM1-2(-300 to-209)containing CompleteHMWEnhancer(-246 to-209)was important for expression level but had no effect on expression specificity in the endosperm.In contrast,CCRM1-1(-208 to-101)was critical for both expression specificity and level of Glu-1.Our findings not only provide new insights to uncover Glu-1 transcription regulatory machinery but also lay foundations for modifying Glu-1 expression. 展开更多
关键词 CONSERVED cis-regulatory modules GLU-1 TRANSCRIPTIONAL regulation Transgenic wheat Triticum AESTIVUM
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Development and identification of a dwarf wheat- Leymus mollis double substitution line with resistance to yellow rust and Fusarium head blight 预览
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作者 Jixin Zhao Yang Liu +7 位作者 Xueni Cheng Yuhui Pang Jiachuang Li Zhenqi Su Jun Wu Qunhui Yang Guihua Bai Xinhong Chen 《作物学报:英文版》 CAS CSCD 2019年第4期516-526,共11页
Leymus mollis (Trin.) Pilger (2n = 4x = 28, NsNsXmXm,), a wild relative of common wheat, possesses many potentially valuable traits for genetic improvement of wheat, including strong, short stems, long spikes with num... Leymus mollis (Trin.) Pilger (2n = 4x = 28, NsNsXmXm,), a wild relative of common wheat, possesses many potentially valuable traits for genetic improvement of wheat, including strong, short stems, long spikes with numerous spikelets, tolerance to drought and cold stresses, and resistance to many fungal and bacterial diseases. In the present study, a wheat-L. mollis double substitution line DM96 was selected from a F6 progeny of a cross between M842-16 (an octoploid Tritileymus line) and D4286 (a Triticum durum line) using genomic in situ hybridization (GISH), simple sequence repeat (SSR) markers, and expressed sequence tagged sequence site (EST-STS) markers. Chromosome analysis at mitosis and meiosis showed that DM96 had a chromosome constitution of 2n = 42 = 21II. GISH analysis indicated that DM96 carried 38 chromosomes from wheat and two homologous pairs of Ns chromosomes from L. mollis. Fluorescent in situ hybridization (FISH) showed that chromosomes 2Ns and 3Ns from L. mollis had replaced wheat chromosomes 2D and 3D in DM96, which was confirmed by SSR and STS markers. The newly developed substitution line DM96 has shorter height, longer spikes and more kernels than its parents and showed high resistance to stripe rust and Fusarium head blight (FHB). Thus, this line is a new bridge material for the production of useful translocation lines for wheat genetic research and genetic improvement of wheat yield and disease resistance in breeding programs. 展开更多
关键词 Disease RESISTANCE DOUBLE substitution line DWARFING Triticum AESTIVUM
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Variation in allelic frequencies at loci associated with kernel weight and their effects on kernel weight-related traits in winter wheat 预览
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作者 Teng Li Hongwei Liu +8 位作者 Chunyan Mai Guangjun Yu Huili Li Lingzhi Meng Dawei Jian Li Yang Yang Zhou Hongjun Zhang Hongjie Li 《作物学报:英文版》 CAS CSCD 2019年第1期30-37,共8页
Knowledge of allelic frequencies at loci associated with kernel weight and effects on kernel weight-related traits is crucial for yield improvement in wheat.Kernel weight-related traits were evaluated in 200 Chinese w... Knowledge of allelic frequencies at loci associated with kernel weight and effects on kernel weight-related traits is crucial for yield improvement in wheat.Kernel weight-related traits were evaluated in 200 Chinese winter wheat cultivars(lines)grown at the Xinxiang Experimental Station,Chinese Academy of Agricultural Sciences,Xinxiang in Henan Province,for three consecutive years from 2014 to 2016.Alleles associated with kernel weight at nine loci,TaCKX6-D1,TaCwi-A1,TaCWI-4A,TaGS1a,TaGS5-A1,TaGS3-3A,TaGW2-6A,TaSus2-2B,and TaTGW6-A1,were determined for all cultivars(lines).ANOVA showed that genotypes,years and their interactions had significant effects on thousand-kernel weight(TKW),kernel length(KL)and kernel width(KW).The overall mean frequencies of alleles conferring high and low TKW at the nine loci were 65.9%and 33.4%,with the ranges of 37.0%–85.0%and 13.5%–63.0%for single loci.The frequencies of high-TKW alleles were over 50.0%at eight of the loci.Genotypes at each locus with the high-TKW allele had higher TKW than those with the low-TKW allele.The high-TKW allele Hap-H at the TaSus2-2B locus can be preferably used to increase grain yield due to its high TKW(49.32 g).A total of 18 main allelic combinations(ACs)at nine loci were detected.Three ACs(AC1–AC3)had significantly higher TKW than AC6 with high-TKW alleles at all nine loci even though they contained some low-TKW alleles.This indicated that other loci controlling kernel weight were present in the high-TKW cultivars.This work provides important information for parental selection and marker-assisted selection for breeding. 展开更多
关键词 Allelic frequency Allelic VARIATION Functional MARKERS TRITICUM AESTIVUM
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SNP-based linkage mapping for validation of adult plant stripe rust resistance QTL in common wheat cultivar Chakwal 86 预览
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作者 Qingdong Zeng Jianhui Wu +8 位作者 Shuo Huang Fengping Yuan Shengjie Liu Qilin Wang Jingmei Mu Shizhou Yu Li Chen Dejun Han Zhensheng Kang 《作物学报:英文版》 CAS CSCD 2019年第2期176-186,共11页
Wheat crops in China are constantly challenged by stripe rust.Deployment of cultivars with diverse resistances is the best strategy to control the disease.A recombinant inbred line (RIL) population derived from a cros... Wheat crops in China are constantly challenged by stripe rust.Deployment of cultivars with diverse resistances is the best strategy to control the disease.A recombinant inbred line (RIL) population derived from a cross between the resistant cultivar Chakwal 86 and susceptible landrace Mingxian 169 was studied in multiple environments to examine the underlying genetics and to identify quantitative trait loci (QTL) for stripe rust resistance.One hundred and twenty-eight RILs were genotyped with wheat 35K SNP array and a genome-wide linkage map with 1480 polymorphic SNP markers,or bins,was constructed.Two major QTL on chromosomes 1BL and 3BS,and one minor QTL on 6BS had significant effects in reducing stripe rust severity.The QTL were validated using composite interval mapping (CIM) and inclusive composite interval mapping (ICIM).These methods explained 59.0%–74.1% of the phenotype variation in disease response.The QTL on chromosome 1BL was confirmed to be Yr29/Lr46 and the one on 3BS was the resistance allele identified in CIMMYT germplasm but was not Yr30/Sr2.The QTL on 6BS probably corresponded to previously known QTL.RILs with combined QTL were more resistant than those with single or no QTL.Kompetitive allele-specific PCR (KASP) assays for the QTL with largest effect QTL on chromosome 3BS were performed on a subset of RILs and 150 unrelated wheat lines.The QTL on 3BS with its linked KASP markers can be used in marker-assisted selection to improve stripe rust resistance in breeding programs. 展开更多
关键词 Genetic linkage HAPLOTYPE analysis QTL mapping PUCCINIA STRIIFORMIS Single nucleotide polymorphism Triticum AESTIVUM
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TaCML36, a wheat calmodulin-like protein, positively participates in an immune response to Rhizoctonia cerealis 预览
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作者 Lin Lu Wei Rong +2 位作者 Ronghua Zhou Naxin Huo Zengyan Zhang 《作物学报:英文版》 CAS CSCD 2019年第5期608-618,共11页
Sharp eyespot,mainly caused by the soil-borne fungus Rhizoctonia cerealis,affects wheat(Triticum aestivum L.)production worldwide.In this study,we isolated TaCML36 gene encoding a wheat calmodulin-like protein,and stu... Sharp eyespot,mainly caused by the soil-borne fungus Rhizoctonia cerealis,affects wheat(Triticum aestivum L.)production worldwide.In this study,we isolated TaCML36 gene encoding a wheat calmodulin-like protein,and studied its defense role in protection against R.cerealis.Transcription of TaCML36 was significantly elevated by both R.cerealis infection and exogenous ethylene treatment.Transcription was higher in resistant wheat lines than in susceptible ones.There were copies of TaCML36 on chromosomes 5A,5B,and 5D.The TaCML36 protein is composed of 183 amino acids and contains two calcium-binding EFhand domains.Subcellular localization assays in wheat indicated that TaCML36 localizes in both the cytoplasm and nucleus.Virus-induced gene silencing and disease assessment indicated that compared to the controls,TaCML36-silenced wheat plants displayed significantly reduced resistance to R.cerealis and had greater fungal biomass,suggesting that knockdown of TaCML36 impaired host resistance.Knockdown of TaCML36 also significantly repressed expression of pathogenesis-related genes such as Chitinase 1,PDF35,and PR17C,the ethylene response factor-encoding gene TaPIE1,and ethylene biosynthesis gene ACO2.Collectively,our results suggest that TaCML36 positively participates in the innate immune response to R.cerealis infection by modulating expression of defense-associated genes possibly in the ethylene signaling pathway. 展开更多
关键词 Calmodulin-like protein Defense-associated gene Ethylene signaling Sharp eyespot Triticum aestivum
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Wheat functional genomics in the era of next generation sequencing: An update 预览
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作者 Meiling Jia Jiantao Guan +4 位作者 Zhiwen Zhai Shuaifeng Geng Xueyong Zhang Long Mao Aili Li 《作物学报:英文版》 CAS CSCD 2018年第1期7-14,共8页
Bread wheat is not only an important cereal crop but also a model for study of an allopolyploid plant with a large, highly repetitive genome. Advances in next-generation sequencing (NGS) technology provide needed thro... Bread wheat is not only an important cereal crop but also a model for study of an allopolyploid plant with a large, highly repetitive genome. Advances in next-generation sequencing (NGS) technology provide needed throughput to conquer the enormous size of the wheat genome. Multiple high quality reference genome sequences will soon be available. Full-scale wheat functional genomics studies are dawning. In this review we highlight the available tools and methodologies for wheat functional genomics research developed with the assistance of NGS technology and recent progress, particularly the concerted effort in generating multiple reference genomes, strategies to attain genome-wide genetic variation, genome-wide association studies, mutant population generation, and NGS-supported gene cloning and functional characterization. These resources and platforms lay a solid foundation for wheat research, leading to a new era of wheat functional genomics that will bridge the gap between genotype and phenotype.Dissection of wheat genomes and gene functions should assist in genomics-assisted selection and facilitate breeding of elite varieties for sustainable agriculture in China and the world. 展开更多
关键词 GWAS NGS TILLING TRITICUM AESTIVUM
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Current advances in genome sequencing of common wheat and its ancestral species 预览
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作者 Xiaoli Shi Hong-Qing Ling 《作物学报:英文版》 CAS CSCD 2018年第1期15-21,共7页
Common wheat is an important and widely cultivated food crop throughout the world.Much progress has been made in regard to wheat genome sequencing in the last decade.Starting from the sequencing of single chromosomes/... Common wheat is an important and widely cultivated food crop throughout the world.Much progress has been made in regard to wheat genome sequencing in the last decade.Starting from the sequencing of single chromosomes/chromosome arms whole genome sequences of common wheat and its diploid and tetraploid ancestors have been decoded along with the development of sequencing and assembling technologies. In this review, we give a brief summary on international progress in wheat genome sequencing, and mainly focus on reviewing the effort and contributions made by Chinese scientists. 展开更多
关键词 Genome SEQUENCING DIPLOID WHEAT TETRAPLOID WHEAT TRITICUM AESTIVUM
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Wheat genome editing expedited by efficient transformation techniques: Progress and perspectives 预览
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作者 Ke Wang Bisma Riaz Xingguo Ye 《作物学报:英文版》 CAS CSCD 2018年第1期22-31,共10页
Genome editing is one of the most promising biotechnologies to improve crop performance.Common wheat is a staple food for mankind. In the past few decades both basic and applied research on common wheat has lagged beh... Genome editing is one of the most promising biotechnologies to improve crop performance.Common wheat is a staple food for mankind. In the past few decades both basic and applied research on common wheat has lagged behind other crop species due to its complex,polyploid genome and difficulties in genetic transformation. Recent breakthroughs in wheat transformation permit a revolution in wheat biotechnology. In this review, we summarize recent progress in wheat genetic transformation and its potential for wheat improvement. We then review recent progress in plant genome editing, which is now readily available in wheat. We also discuss measures to further increase transformation efficiency and potential applications of genome editing in wheat. We propose that, together with a high quality reference genome, the time for efficient genetic engineering and functionality studies in common wheat has arrived. 展开更多
关键词 TRITICUM AESTIVUM GENOME EDITING CRISPR/Cas9 Genetic TRANSFORMATION
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Genetic improvement of heat tolerance in wheat:Recent progress in understanding the underlying molecular mechanisms 预览
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作者 Zhongfu Ni Hongjian Li +4 位作者 Yue Zhao Huiru Peng Zhaorong Hu Mingming Xin Qixin Sun 《作物学报:英文版》 CAS CSCD 2018年第1期32-41,共10页
As a cool season crop, wheat (Triticum aestivum L.) has an optimal daytime growing temperature of 15 °C during the reproductive stage. With global climate change, heat stress is becoming an increasingly severe co... As a cool season crop, wheat (Triticum aestivum L.) has an optimal daytime growing temperature of 15 °C during the reproductive stage. With global climate change, heat stress is becoming an increasingly severe constraint on wheat production. In this review, we summarize recent progress in understanding the molecular mechanisms of heat tolerance in wheat. We firstly describe the impact of heat tolerance on morphology and physiology and its potential effect on agronomic traits. We then review recent discoveries in determining the genetic and molecular factors affecting heat tolerance, including the effects of phytohormone signaling and epigenetic regulation. Finally, we discuss integrative strategies to improve heat tolerance by utilization of existing germplasm including modern cultivars, landraces and related species. 展开更多
关键词 Heat stress PHYTOHORMONE signaling EPIGENETIC regulation TRITICUM AESTIVUM
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The landscape of molecular mechanisms for salt tolerance in wheat 预览
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作者 Meng Wang Guangmin Xia 《作物学报:英文版》 CAS CSCD 2018年第1期42-47,共6页
Wheat is one of the most important food crops, and its yield is seriously restricted by high salinity and other abiotic stresses. Many attempts have been made to elucidate the major physiological processes associated ... Wheat is one of the most important food crops, and its yield is seriously restricted by high salinity and other abiotic stresses. Many attempts have been made to elucidate the major physiological processes associated with salt tolerance and to identify the genes controlling the processes. In this review, the major role of high-affinity potassium transporter (HKT)genes in enhancing the salt tolerance of wheat is summarized. The link between maintenance of reactive oxygen species (ROS) homeostasis and salt tolerance through a comprehensive study of a wheat introgression line is examined, and the contribution of a set of genes involved in this process is depicted. New research strategies to uncover the mechanisms underlying salt tolerance in wheat based on recent advances in omics will be discussed. 展开更多
关键词 IONIC and ROS HOMEOSTASIS Molecular BREEDING Salt tolerance TRITICUM AESTIVUM
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A journey to understand wheat Fusarium head blight resistance in the Chinese wheat landrace Wangshuibai 预览
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作者 Haiyan Jia Jiyang Zhou +9 位作者 Shulin Xue Guoqiang Li Haisheng Yan Congfu Ran Yiduo Zhang Jinxing Shi Li Jia Xin Wang Jing Luo Zhengqiang Ma 《作物学报:英文版》 CAS CSCD 2018年第1期48-59,共12页
Fusarium head blight (FHB) or scab caused by Fusarium graminearum is a major threat to wheat production in China as well as in the world. To combat this disease, multiple efforts have been carried out internationally.... Fusarium head blight (FHB) or scab caused by Fusarium graminearum is a major threat to wheat production in China as well as in the world. To combat this disease, multiple efforts have been carried out internationally. In this article, we review our long-time effort in identifying the resistance genes and dissecting the resistance mechanisms by both forward and reverse genetics approaches in the last two decades. We present recent progress in resistance QTLidentification, candidate functional gene discovery, marker-assisted improvement of FHB resistant varieties, and findings in investigating association of signal molecules, such as Ca++,SA, JA, and ET, with FHB response, with the assistance from rapidly growing genomics platforms. The information will be helpful for designing novel and efficient approaches to curb FHB. 展开更多
关键词 FUSARIUM head blight QTL Gene discovery Marker-assisted selection TRITICUM AESTIVUM
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Mapping stripe rust resistance genes by BSR-Seq:YrMM58 and YrHY1 on chromosome 2AS in Chinese wheat lines Mengmai 58 and Huaiyang 1 are Yr17 预览
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作者 Yong Wang Huaizhi Zhang +15 位作者 Jingzhong Xie Bingmin Guo Yongxing Chen Huaiyu Zhang Ping Lu Qiuhong Wu Miaomiao Li Deyun Zhang Guanghao Guo Jian Yang Panpan Zhang Yan Zhang Xicheng Wang Hong Zhao Tingjie Cao Zhiyong Liu 《作物学报:英文版》 CAS CSCD 2018年第1期91-98,共8页
Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating fungal diseases in common wheat (Triticum aestivum L.) in China and worldwide. Resistance breeding is the ... Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating fungal diseases in common wheat (Triticum aestivum L.) in China and worldwide. Resistance breeding is the most effective strategy to control diseases in crop plants. Chinese wheat lines Mengmai 58 and Huaiyang 1 are highly resistant to PST race CYR34 (V26) at the adult plant stage. To genetically map the underlying resistance genes we developed segregating populations by crossing Mengmai 58 and Huaiyang 1 with the susceptible cultivar Nongda 399. The stripe rust resistances in Mengmai 58 and Huaiyang 1 were both controlled by single dominant genes, provisionally designated YrMM58 and YrHY1, respectively. Bulked segregant RNA-Seq (BSR-Seq) analysis showed that YrMM58 and YrHY1 were located in the same distal ~16 Mb region on chromosome 2AS.Comparative genomics analysis with the physical map of Aegilops tauschii proved useful for developing additional markers to saturate the genetic linkage map. YrMM58 and YrHY1 were mapped to the distal end of chromosome arm 2AS, with the closest marker WGGB148 being 7.7 cM and 3.8 cM from the resistance gene, which was considered to be Yr17. These markers can be used in marker-assisted selection. 展开更多
关键词 Genetic MAPPING STRIPE rust TRITICUM AESTIVUM BSR-seq SNP
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Molecular mapping of a novel wheat powdery mildew resistance gene Ml92145E8-9 and its application in wheat breeding by marker-assisted selection 预览
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作者 Xiaocheng Yu Shizhong Ren +8 位作者 Lanfei Zhao Jun Guo Yinguang Bao Yingxue Ma Hongwei Wang Herbert W.Ohm Dazhao Yu Hongjie Li Lingrang Kong 《作物学报:英文版》 CAS CSCD 2018年第6期621-627,共7页
Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is one of the most devastating diseases of common wheat(Triticum aestivum L.).The wheat line 92145E8-9 is immune to Bgt isolate E09.Genetic analysis reveals... Powdery mildew,caused by Blumeria graminis f.sp.tritici(Bgt),is one of the most devastating diseases of common wheat(Triticum aestivum L.).The wheat line 92145E8-9 is immune to Bgt isolate E09.Genetic analysis reveals that the powdery mildew resistance in 92145E8-9 is controlled by a single dominant gene,temporarily designated Ml92145E8-9.Bulkedsegregant analysis(BSA)with simple sequence repeat(SSR)markers indicates that Ml92145E8-9 is located on chromosome 2AL.According to the reactions of 92145E8-9,VPM1(Pm4b carrier),and Lankao 906(PmLK906 carrier)to 14 Bgt isolates,the resistance spectrum of 92145E8-9 differs from those of Pm4b and PmLK906,both of which were previously localized to 2AL.To test the allelism among Ml92145E8-9,Pm4b and PmLK906,two F2 populations of 92145E8-9×VPM1(Pm4b)and 92145E8-9×Lankao 906(PmLK906)were developed in this study.Screening of 784 F2 progeny of 92145E8-9×VPM1 and 973 F2 progeny of 92145E8-9×Lankao 906 for Bgt isolate E09 identified 37 and 19 susceptible plants,respectively.These findings indicated that Ml92145E8-9 is non-allelic to either Pm4b or PmLK906.Thus,Ml92145E8-9 is likely to be a new powdery mildew resistance gene on 2AL.New polymorphic markers were developed based on the collinearity of genomic regions of Ml92145E8-9 with the reference sequences of the International Wheat Genome Sequencing Consortium(IWGSC).Ml92145E8-9 was mapped to a 3.6 cM interval flanked by molecular markers Xsdauk13 and Xsdauk682.This study also developed five powdery mildew-resistant wheat lines(SDAU3561,SDAU3562,SDAU4173,SDAU4174,and SDAU4175)using flanking marker-aided selection.The markers closely linked to Ml92145E8-9 would be useful in marker-assisted selection for wheat powdery mildew resistance breeding. 展开更多
关键词 Marker-assisted SELECTION Ml92145E8-9 Powdery MILDEW TRITICUM AESTIVUM L.
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A catalog of gliadin alleles:Polymorphism of 20th-century common wheat germplasm 预览
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作者 Eugene Metakovsky Viktor Melnik +2 位作者 Marta Rodriguez-Quijano Vladimir Upelniek Jose Maria Carrillo 《作物学报:英文版》 CAS CSCD 2018年第6期628-641,共14页
A new,improved version of the catalog of 182 alleles at the six Gli loci of common wheat(T.aestivum L.)shown in electrophoregrams of 128 standard genotypes was used for analysis of 1060 cultivars and lines bred in the... A new,improved version of the catalog of 182 alleles at the six Gli loci of common wheat(T.aestivum L.)shown in electrophoregrams of 128 standard genotypes was used for analysis of 1060 cultivars and lines bred in the 20th century.The most frequent alleles in the studied germplasm occurred with frequencies of 18%–40%,with 30 unique alleles,one in each cultivar.Extremely high genetic diversity was found(average H for the six main Gli loci was 0.870±0.046),nearly identical in winter(H=0.831)and spring(H=0.856)wheats but differing among 28 groups of cultivars released in 22 countries.Each country or region was characterized by its own specific set of the most frequent Gli alleles,and the 28 cultivar groups formed five main relationship clusters if polymorphism at the six Gli loci was considered.However,different levels of similarity between groups of cultivars were found if polymorphism of the A,B,or D genomes of common wheat was tested separately.In general,the 20th century germplasm of common wheat was differentiated and structured by country or region and cultivar type(spring or winter).Each elemental genome(in particular,A and D)contributed to the structure of the polymorphism studied.We propose that the structure of the wheat germplasm was a result of natural selection under the ecoclimatic conditions of cultivation specific to each country or region.As many as 27.4%of cultivars studied violated the requirement of the DUS rules for uniformity,being represented by mixtures of two or more closely related genotypes.However,the composition of a cultivar as a set of related but different genotypes may contribute to its adaptivity,and thereby to the known high plasticity of common wheat. 展开更多
关键词 GLIADIN alleles Genetic variation Gene geography Intra-varietal NON-UNIFORMITY ALLOPOLYPLOID TRITICUM AESTIVUM
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