期刊文献+
共找到4,111篇文章
< 1 2 206 >
每页显示 20 50 100
Early active immunization with Aβ3–10-KLH vaccine reduces tau phosphorylation in the hippocampus and protects cognition of mice 预览
1
作者 Jin-Chun Wang Kun Zhu +3 位作者 Hui-Yi Zhang Guo-Qing Wang Hui-Ying Liu Yun-Peng Cao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期519-527,共9页
Active and passive anti-Aβimmunotherapies have successfully been used for the prevention and treatment of Alzheimer’s disease animal models.However,clinical use of these immunotherapies is not effective,because the ... Active and passive anti-Aβimmunotherapies have successfully been used for the prevention and treatment of Alzheimer’s disease animal models.However,clinical use of these immunotherapies is not effective,because the vaccination is administered too late.At 1 month of age,100μL of Aβ3–10-KLH peptide(vaccine,2μg/μL)was subcutaneously injected into the neck of an amyloid precursor protein/presenilin-1/tau transgenic(3×Tg-AD)mouse model.Aβ3–10-KLH peptide was re-injected at 1.5,2.5,3.5,4.5,5.5,and 6.5 months of age.Serum levels of Aβantibody were detected by enzyme-linked immunosorbent assay,while spatial learning and memory ability were evaluated by Morris water maze.Immunohistochemistry was used to detect total tau with HT7 and phosphorylated tau with AT8(phosphorylation sites Ser202 and Thr205)and AT180(phosphorylation site Thr231)antibodies in the hippocampus.In addition,western blot analysis was used to quantify AT8 and AT180 expression in the hippocampus.The results showed that after vaccine injection,mice produced high levels of Aβantibody,cognitive function was significantly improved,and total tau and phosphorylated tau levels were significantly reduced.These findings suggest that early active immunization with Aβ3–10-KLH vaccine can greatly reduce tau phosphorylation,thereby mitigating the cognitive decline of 3×Tg-AD mice.This study was approved by the Animal Ethics Committee of China Medical University,China(approval No.103-316)on April 2,2016. 展开更多
关键词 3×Tg-AD Aβ3–10-KLH VACCINE Alzheimer’s disease amyloid precursor protein AMYLOID-BETA cognitive DECLINE tau phosphorylation transgenic mouse
在线阅读 下载PDF
δ-Opioid receptor as a potential therapeutic target for ischemic stroke 预览
2
作者 Kalpana Subedi Hongmin Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第1期20-24,共5页
Ischemic stroke is a global epidemic condition due to an inadequate supply of blood and oxygen to a specific area of brain either by arterial blockage or by narrowing of blood vessels.Despite having advancement in the... Ischemic stroke is a global epidemic condition due to an inadequate supply of blood and oxygen to a specific area of brain either by arterial blockage or by narrowing of blood vessels.Despite having advancement in the use of thrombolytic and clot removal medicine,significant numbers of stroke patients are still left out without option for treatment.In this review,we summarize recent research work on the activation ofδ-opioid receptor as a strategy for treating ischemic stroke-caused neuronal injury.Moreover,as activation ofδ-opioid receptor by a non-peptidicδ-opioid receptor agonist also modulates the expression,maturation and processing of amyloid precursor protein andβ-secretase activity,the potential role of these effects on ischemic stroke caused dementia or Alzheimer’s disease are also discussed. 展开更多
关键词 AGONIST AKT AMYLOID precursor protein BDNF ischemic stroke NEUROPROTECTION δ-opioid receptor p38 MAPK PI3K TRKB
在线阅读 下载PDF
Navigating the dynamic landscape of alpha-synuclein morphology:a review of the physiologically relevant tetrameric conformation 预览
3
作者 Heather RLucas Ricardo DFernández 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期407-415,共9页
N-acetylatedα-synuclein(αSyn)has long been established as an intrinsically disordered protein associated with a dysfunctional role in Parkinson’s disease.In recent years,a physiologically relevant,higher order conf... N-acetylatedα-synuclein(αSyn)has long been established as an intrinsically disordered protein associated with a dysfunctional role in Parkinson’s disease.In recent years,a physiologically relevant,higher order conformation has been identified as a helical tetramer that is tailored by buried hydrophobic interactions and is distinctively aggregation resistant.The canonical mechanism by which the tetramer assembles remains elusive.As novel biochemical approaches,computational methods,pioneering purification platforms,and powerful imaging techniques continue to develop,puzzling information that once sparked debate as to the veracity of the tetramer has now shed light upon this new counterpart inαSyn neurobiology.Nuclear magnetic resonance and computational studies on multimericαSyn structure have revealed that the protein folding propensity is controlled by small energy barriers that enable large scale reconfiguration.Alternatively,familial mutations ablate tetramerization and reconfigure polymorphic fibrillization.In this review,we will discuss the dynamic landscape ofαSyn quaternary structure with a focus on the tetrameric conformation. 展开更多
关键词 ALPHA-SYNUCLEIN amyloid FIBRILS intrinsically disordered PROTEIN MULTIMER N-ACETYLATION oligomer Parkinson’s disease PROTEIN folding PROTEIN structure TETRAMER
在线阅读 下载PDF
Administration of pre/probiotics with conventional drug treatment in Alzheimer’s disease 预览
4
作者 Jakub Hort Martin Valis Francesco Angelucci 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期448-449,共2页
Alzheimer’s disease(AD)is a neurodegenerative disease with long preclinical phase,typically followed by a slow decline in memory,thinking and reasoning abilities.The underlying pathological processes are not yet full... Alzheimer’s disease(AD)is a neurodegenerative disease with long preclinical phase,typically followed by a slow decline in memory,thinking and reasoning abilities.The underlying pathological processes are not yet fully elucidated,although two main disease hallmarks have been associated with AD pathology:amyloid beta species and neurofibrillary tangles.The plaques are deposits of a protein fragment called beta-amyloid(Aβ),which accumulates in the spaces between neurons.Tangles are twisted fibers of the tau protein,which accumulates inside the cells. 展开更多
关键词 ALZHEIMER AMYLOID clinical
在线阅读 下载PDF
淀粉样蛋白A、C-反应蛋白与动脉硬化的相关性研究 预览
5
作者 曾俊超 姚冠勇 +9 位作者 徐信 但超 李慧卉 李芳 刘峻宇 郝婷 曾美红 范嫣 陈丹妮 杨锐 《国际检验医学杂志》 CAS 2020年第4期446-449,453,共5页
目的研究淀粉样蛋白A、C-反应蛋白与动脉硬化的关系。方法收集2018年6月至2019年1月该院体检的人群749例,其中动脉硬化程度升高者360例,动脉硬化程度正常者389例,年龄18~84岁。评估体检人群的动脉硬化程度,检测并定量血清中淀粉样蛋白A... 目的研究淀粉样蛋白A、C-反应蛋白与动脉硬化的关系。方法收集2018年6月至2019年1月该院体检的人群749例,其中动脉硬化程度升高者360例,动脉硬化程度正常者389例,年龄18~84岁。评估体检人群的动脉硬化程度,检测并定量血清中淀粉样蛋白A。Logistic回归分析淀粉样蛋白A和C-反应蛋白与动脉硬化的关系。限制性3次样条分析进一步研究自变量与因变量的非线性关系。结果在未调整模型中,动脉硬化组中的C-反应蛋白和淀粉样蛋白A高于对照组(均P<0.05);充分调整协变量后两组间的C-反应蛋白水平差异相对变小(P=0.041),而两组间的淀粉样蛋白A水平差异仍然较大(P=0.003)。单因素Logistic回归发现C-反应蛋白和淀粉样蛋白A均与动脉硬化风险呈正相关,95%CI分别为3.299(1.575~6.910)、2.798(1.670~4.689)。多重Logistic回归发现未调整协变量的淀粉样蛋白A与动脉硬化风险呈正相关,95%CI为2.191(1.165~4.119),而C-反应蛋白与动脉硬化风险无关,这种趋势在充分调整协变量后仍然存在。限制性3次样条回归表明随着淀粉样蛋白A的升高,动脉硬化风险的变化呈现为类似于"指数"的增长模式。结论淀粉样蛋白A与动脉硬化存在正相关关系,而C-反应蛋白与动脉硬化的关系可能依赖于淀粉样蛋白A。 展开更多
关键词 淀粉样蛋白A C-反应蛋白 动脉硬化
在线阅读 下载PDF
Natural stilbenes effects in animal models of Alzheimer’s disease 预览
6
作者 Aline Freyssin Guylène Page +1 位作者 Bernard Fauconneau Agnès Rioux Bilan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第5期843-849,共7页
Alzheimer’s disease is one of the most frequent neurodegenerative diseases.This pathology is characterized by protein aggregates,mainly constituted by amyloid peptide and tau,leading to neuronal death and cognitive i... Alzheimer’s disease is one of the most frequent neurodegenerative diseases.This pathology is characterized by protein aggregates,mainly constituted by amyloid peptide and tau,leading to neuronal death and cognitive impairments.Drugs currently proposed to treat this pathology do not prevent neurodegenerative processes and are mainly symptomatic therapies.However,stilbenes presenting multiple pharmacological effects could be good potential therapeutic candidates.The aim of this review is to gather the more significant papers among the broad literature on this topic,concerning the beneficial effects of stilbenes (resveratrol derivatives) in animal models of Alzheimer’s disease.Indeed,numerous studies focus on cellular models,but an in vivo approach remains of primary importance since in animals (mice or rats,generally),bioavailability and metabolism are taken into account,which is not the case in in vitro studies.Furthermore,examination of memory ability is feasible in animal models,which strengthens the relevance of a compound with a view to future therapy in humans.This paper is addressed to any researcher who needs to study untested natural stilbenes or who wants to experiment the most effective natural stilbenes in largest animals or in humans.This review shows that resveratrol,the reference polyphenol,is largely studied and seems to have interesting properties on amyloid plaques,and cognitive impairment.However,some resveratrol derivatives such as gnetin C,trans-piceid,or astringin have never been tested on animals.Furthermore,pterostilbene is of particular interest,by its improvement of cognitive disorders and its neuroprotective role.It could be relevant to evaluate this molecule in clinical trials. 展开更多
关键词 Alzheimer's disease AMYLOID animal models cognitive impairment inflammation NATURAL STILBENES NEUROPROTECTION RESVERATROL tau
在线阅读 下载PDF
2型糖尿病与阿尔兹海默症的相关性研究 预览
7
作者 李佳 欧思琳 李茹冰 《暨南大学学报:自然科学与医学版》 CAS CSCD 北大核心 2019年第5期436-443,共8页
流行病学资料显示2型糖尿病(T2DM)患者的阿尔兹海默症(AD)发病率较高,认为T2DM是AD发展的重要危险因素.AD和T2DM分别以脑淀粉样蛋白-β(Aβ)和胰岛淀粉样多肽(hIAPP)沉积为特征.淀粉样变蛋白在这两种疾病之间有着相互的作用.本文对现有... 流行病学资料显示2型糖尿病(T2DM)患者的阿尔兹海默症(AD)发病率较高,认为T2DM是AD发展的重要危险因素.AD和T2DM分别以脑淀粉样蛋白-β(Aβ)和胰岛淀粉样多肽(hIAPP)沉积为特征.淀粉样变蛋白在这两种疾病之间有着相互的作用.本文对现有的相关研究进行总结,旨在更好地了解AD和T2DM之间的相关性,从而为下一步的治疗提供一些参考. 展开更多
关键词 2型糖尿病 阿尔兹海默症 淀粉样变蛋白 胰岛淀粉样多肽 脑淀粉样蛋白-β
在线阅读 免费下载
miR-15b-5p targeting amyloid precursor protein is involved in the anti-amyloid eflect of curcumin in swAPP695-HEK293 cells 预览
8
作者 Hong-Ying Liu Xian Fu +4 位作者 You-Fu Li Xian-Liang Li Zhen-Yu Ma Ying Zhang Qing-Chun Gao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1603-1609,共7页
Curcumin exerts a neuroprotective effect on Alzheimer’s disease;however,it is not known whether microRNAs are involved in this protective effect.This study was conducted using swAPP695-HEK293 cells as an Alzheimer’s... Curcumin exerts a neuroprotective effect on Alzheimer’s disease;however,it is not known whether microRNAs are involved in this protective effect.This study was conducted using swAPP695-HEK293 cells as an Alzheimer’s disease cell model.swAPP695-HEK293 cells were treated with 0,0.5,1,2,5,and 10μM curcumin for 24 hours.The changes in miR-15b-5p,miR-19a-3p,miR-195-5p,miR-101-3p,miR-216b-5p,miR-16-5p and miR-185-5p expression were assessed by real-time quantitative polymerase chain reaction.The mRNA and protein levels of amyloid precursor protein,amyloid-β40 and amyloid-β42 were evaluated by quantitative real-time polymerase chain reaction,western blot assays and enzyme-linked immunosorbent assays.swAPP695-HEK293 cells were transfected with miR-15b-5p mimic,or treated with 1μM curcumin 24 hours before miR-15b-5p inhibitor transfection.The effects of curcumin on amyloid precursor protein,amyloid-β40 and amyloid-β42 levels were evaluated by western blot assays and enzyme-linked immunosorbent assay.Luciferase assays were used to analyze the interaction between miR-15b-5p and the 3′-untranslated region of amyloid precursor protein.The results show that amyloid precursor protein and amyloid-βexpression were enhanced in swAPP695-HEK293 cells compared with HEK293 parental cells.Curcumin suppressed the expression of amyloid precursor protein and amyloid-βand up-regulated the expression of miR-15b-5p in swAPP695-HEK293 cells.In addition,we found a negative association of miR-15b-5p expression with amyloid precursor protein and amyloid-βlevels in the curcumin-treated cells.Luciferase assays revealed that miR-15b-5p impaired the luciferase activity of the plasmid harboring the 3′-untranslated region of amyloid precursor protein.These findings indicate that curcumin down-regulates the expression of amyloid precursor protein and amyloid-βin swAPP695-HEK293 cells,which was partially mediated by miR-15b-5p via targeting of the 3′-untranslated region of amyloid precursor protein. 展开更多
关键词 nerve REGENERATION Alzheimer’s disease natural plant drug CURCUMINOIDS miRNAs AMYLOID precursor protein amyloid 3′-untranslated region LUCIFERASE assays neurons neural REGENERATION
在线阅读 下载PDF
Physiological effects of amyloid precursor protein and its derivatives on neural stem cell biology and signaling pathways involved 预览
9
作者 Raquel Coronel Charlotte Palmer +4 位作者 Adela Bernabeu-Zornoza María Monteagudo Andreea Rosca Alberto Zambrano Isabel Liste 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1661-1671,共11页
The pathological implication of amyloid precursor protein(APP)in Alzheimer’s disease has been widely documented due to its involvement in the generation of amyloid-β peptide.However,the physiological functions of AP... The pathological implication of amyloid precursor protein(APP)in Alzheimer’s disease has been widely documented due to its involvement in the generation of amyloid-β peptide.However,the physiological functions of APP are still poorly understood.APP is considered a multimodal protein due to its role in a wide variety of processes,both in the embryo and in the adult brain.Specifically,APP seems to play a key role in the proliferation,differentiation and maturation of neural stem cells.In addition,APP can be processed through two canonical processing pathways,generating different functionally active fragments:soluble APP-α,soluble APP-β,amyloid-β peptide and the APP intracellular C-terminal domain.These fragments also appear to modulate various functions in neural stem cells,including the processes of proliferation,neurogenesis,gliogenesis or cell death.However,the molecular mechanisms involved in these effects are still unclear.In this review,we summarize the physiological functions of APP and its main proteolytic derivatives in neural stem cells,as well as the possible signaling pathways that could be implicated in these effects.The knowledge of these functions and signaling pathways involved in the onset or during the development of Alzheimer’s disease is essential to advance the understanding of the pathogenesis of Alzheimer’s disease,and in the search for potential therapeutic targets. 展开更多
关键词 AMYLOID precursor protein APP SOLUBLE APP alpha SOLUBLE APP BETA AMYLOID BETA peptide APP intracellular domain NEURAL stem CELLS NEURAL progenitor CELLS neurogenesis signaling pathways
在线阅读 下载PDF
银杏胚乳淀粉体的积累规律和发生特性 预览
10
作者 陆彦 郝唯卓 +5 位作者 潘烨 张晓敏 祁琰 凌裕平 金飚 王莉 《植物科学学报》 CAS CSCD 北大核心 2019年第6期788-796,共9页
以银杏(Ginkgo biloba L.)核用品种‘七星果’、‘马铃’和‘龙眼’不同发育天数的胚乳为材料,采用透射电镜和扫描电镜技术,对其胚乳细胞内淀粉体的积累规律和发生特性进行研究。结果显示:3种银杏胚乳形态差异显著,‘七星果’呈梭形、... 以银杏(Ginkgo biloba L.)核用品种‘七星果’、‘马铃’和‘龙眼’不同发育天数的胚乳为材料,采用透射电镜和扫描电镜技术,对其胚乳细胞内淀粉体的积累规律和发生特性进行研究。结果显示:3种银杏胚乳形态差异显著,‘七星果’呈梭形、‘马铃’呈椭圆形、‘龙眼’呈卵圆形;3种银杏胚乳早期均为嫩绿色,后期为黄色;授粉后65~125 d是胚乳体积快速增长时期。淀粉体的积累规律为:在胚乳组织内,淀粉体由糊粉层-外胚乳-内胚乳逐渐积累;在单个胚乳细胞内,淀粉体由细胞壁边缘向内部逐渐充实。银杏淀粉质体起源于类叶绿体质体,淀粉粒最初在类叶绿体质体的内膜上发生。淀粉体通过出芽、缢缩以及出芽和缢缩同时进行的增殖方式产生新淀粉体,成熟淀粉体形态有圆形、椭圆形和不规则形,属于单粒淀粉。研究结果表明银杏淀粉体在胚乳组织内具有由外向内的空间积累规律,淀粉质体起源于类叶绿体质体并通过出芽、缢缩、出芽和缢缩同时存在的方式增殖。 展开更多
关键词 银杏 胚乳 淀粉体 积累规律 发生特性
在线阅读 免费下载
Potential preventive disease-modifying pharmacological strategies to delay late onset Alzheimer’s disease 预览
11
作者 Miren Ettcheto Oriol Busquets Antoni Camins 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1721-1725,共5页
Alzheimer’s disease(AD)is a progressive neurodegenerative disease that was histopathologically characterized in the brain by the presence of extracellular senile plaques made of amyloid β peptides and intracellular ... Alzheimer’s disease(AD)is a progressive neurodegenerative disease that was histopathologically characterized in the brain by the presence of extracellular senile plaques made of amyloid β peptides and intracellular neurofibrillary tangles composed of hyperphosphorylated Tau protein.Over the years,AD has been classified in two subgroups:early onset or familial AD and late onset or sporadic AD.On the one hand,familial AD has been described to be the result of genetic mutations that cause,in some cases,for the overproduction of amyloid β.On the other,the cause of late onset or sporadic AD is still unclear even though several hypotheses have been proposed to explain the process of severe and progressive memory and cognitive loss.In the present review,some of the current hypotheses that try to explain the origin of late onset or sporadic AD have been summarized.Also,their potential implication in the development of new drugs for the presymptomatic treatment of late onset or sporadic AD has been considered. 展开更多
关键词 Alzheimer's DISEASE BETA-SECRETASE NEUROINFLAMMATION Tau amyloid N-METHYL-D-ASPARTATE glutamate
在线阅读 下载PDF
Physical and toxicological profiles of human IAPP amyloids and plaques
12
作者 Aleksandr Kakinen Yunxiang Sun +9 位作者 Ibrahim Javed Ava Faridi Emily H.Pilkington Pouya Faridi Anthony W.Purcell Ruhong Zhou Feng Ding Sijie Lin Pu Chun Ke Thomas P.Davis 《科学通报:英文版》 SCIE EI CSCD 2019年第1期26-35,共10页
Although much has been learned about the fibrillization kinetics,structure and toxicity of amyloid proteins,the properties of amyloid fibrils beyond the saturation phase are often perceived as chemically and biologica... Although much has been learned about the fibrillization kinetics,structure and toxicity of amyloid proteins,the properties of amyloid fibrils beyond the saturation phase are often perceived as chemically and biologically inert,despite evidence suggesting otherwise.To fill this knowledge gap,we examined the physical and biological characteristics of human islet amyloid polypeptide (IAPP)fibrils that were aged up to two months.Not only did aging decrease the toxicity of IAPP fibrils,but the fibrils also sequestered fresh IAPP and suppressed their toxicity in an embryonic zebrafish model.The mechanical properties of IAPP fibrils in different aging stages were probed by atomic force microscopy and sonication,which displayed comparable stiffness but age-dependent fragmentation,followed by self-assembly of such fragments into the largest lamellar amyloid structures reported to date.The dynamic structural and toxicity profiles of amyloid fibrils and plaques suggest that they play active,long-term roles in cell degeneration and may be a therapeutic target for amyloid diseases. 展开更多
关键词 IAPP AMYLOID PLAQUE Self assembly Toxicity
The Theory of Dove-like Particles 预览
13
作者 SUN Zuodong 《美中医学:英文版》 2019年第2期73-99,共27页
Objective:Alzheimer‘s disease(AD)has been reported for more than 100 years since its first discovery in 1906.There has been no significant progress in the study of its real causes and pathogenesis.The viewpoint of th... Objective:Alzheimer‘s disease(AD)has been reported for more than 100 years since its first discovery in 1906.There has been no significant progress in the study of its real causes and pathogenesis.The viewpoint of this paper is a heuristic viewpoint based on brain cell activation theory under such background.In this paper,the pathogenesis of sporadic AD is discussed at molecular level by applying the principles of cell physics and biology.The purpose of this paper is to harmonize the existing theories of etiology of AD and to solve the source problems that have plagued the research field of neurodegenerative diseases for a long time.Method:(1)Discuss the relationship with the existing hypothesis:the Aβprotein hypothesis,the tau protein hypothesis,the presenilin(PS)hypothesis,the apolipoprotein E(ApoE)hypothesis,the cholinergic hypothesis,the inflammatory hypothesis;(2)Demonstration:biophysical proof,medical pathological proof,biological model proof;(3)Interpretation:ion channel and blood-brain barrier,potassium ion and potassium channel,ion pump and epilepsy and cancer,A beta protein and spots and plaques,related AD solutions.Result:(1)Abeta is not the cause of AD,but the remains of brain cells after abnormal apoptosis;(2)The K^+concentration difference of 0.00001%which causes the great change of membrane potential is the effective concentration,which can not be neglected;(3)Abnormal impairment of potassium channels and early entry of sodium ions to occupy potassium positions are related to epilepsy,cancer and HeLa cells;(4)AD is a physical disease,especially transcranial magnetoelectricity stimulation,should be the first choice for treatment,which can activate abnormal neurons accurately without interfering with normal neurons.Conclusion:(1)Basic contents:excess cations are transferred from extracellular to intracellular.They compete position with potassium ions on the inner surface of cell membranes,thus abatementing the membrane potential,making action potential unable to activate calcium channels normally, 展开更多
关键词 Alzheimer ETIOLOGY CATIONIC PLACEMENT AMYLOID PLAQUE cell REMAINS physical mean.
在线阅读 下载PDF
血清降钙素原、C反应蛋白、淀粉样蛋白在鉴别诊断小儿肠炎中的价值 预览
14
作者 张小佛 王曼知 +2 位作者 危松青 李嘉 吴蕾 《安徽医药》 CAS 2019年第10期2044-2046,I0005共4页
目的探讨检测血清降钙素原(PCT)、C反应蛋白(CRP)、淀粉样蛋白(SAA)鉴别诊断小儿肠炎的临床价值。方法回顾性选取2016年3月至2017年9月长沙市中心医院细菌感染性腹泻病儿100例(细菌组)、病毒感染致腹泻病儿100例(病毒组),检测两组病儿血... 目的探讨检测血清降钙素原(PCT)、C反应蛋白(CRP)、淀粉样蛋白(SAA)鉴别诊断小儿肠炎的临床价值。方法回顾性选取2016年3月至2017年9月长沙市中心医院细菌感染性腹泻病儿100例(细菌组)、病毒感染致腹泻病儿100例(病毒组),检测两组病儿血清PCT、CRP、SAA水平,并分析这三种指标单独及联合应用于临床鉴别诊断细菌、病毒感染腹泻病儿的价值。结果细菌组的血清PCT、CRP、SAA、外周血白细胞(WBC)、中性粒细胞(N%)水平显著的高于病毒组,差异有统计学意义(P<0.05);血清PCT鉴别诊断细菌性和病毒性肠炎的临界值为1.02μg/L,诊断的灵敏度为88.12%、特异度为93.17%、受试者工作特征曲线(ROC曲线)下面积(AUC)值为0.908;血清CRP鉴别诊断细菌性和病毒性肠炎的临界值为15.8 mg/L,诊断的灵敏度为78.63%、特异度为85.04%、AUC值为0.829;血清SAA鉴别诊断细菌性和病毒性肠炎的临界值为155.2 mg/L,诊断的灵敏度为81.38%、特异度为87.29%、AUC值为0.843;PCT+CRP+SAA鉴别诊断细菌性和病毒性肠炎的灵敏度为91.44%、特异度为96.91%、漏诊率为8.56%、误诊率为3.09%、AUC值为0.946。结论PCT对细菌性和病毒性肠炎的诊断价值高于CRP、SAA,三者联合应用具有更高的诊断价值。 展开更多
关键词 肠炎 腹泻 儿童 学龄前 婴儿 降钙素原 C反应蛋白 淀粉样蛋白
在线阅读 下载PDF
丹参多酚酸盐注射液对心绞痛患者血清相关因子表达的影响 预览
15
作者 乔尚 郭曙军 高雯 《重庆医学》 CAS 2019年第A01期169-171,共3页
目的观察丹参多酚酸盐治疗心绞痛疗效及其对细胞间黏附因子-1(ICM-1)、C反应蛋白(CRP)和淀粉样蛋白A(SAA)的影响。方法将80例不稳定型心绞痛患者分为对照组和治疗组,每组40例。对照组采用常规治疗,治疗组在常规治疗基础上加用丹参多酚... 目的观察丹参多酚酸盐治疗心绞痛疗效及其对细胞间黏附因子-1(ICM-1)、C反应蛋白(CRP)和淀粉样蛋白A(SAA)的影响。方法将80例不稳定型心绞痛患者分为对照组和治疗组,每组40例。对照组采用常规治疗,治疗组在常规治疗基础上加用丹参多酚酸盐静脉滴注,两组均连续治疗14d,观察心绞痛发作次数、心电图变化等,采用酶联免疫吸附法测定两组患者治疗前后ICM-1、CRP和SAA的变化。结果治疗组患者心绞痛次数明显低于对照组,差异有统计学意义(P<0.05);心电图变化与对照组比较,差异无统计学意义(P>0.05);血清ICM-1、CRP和SAA水平均明显低于对照组,差异均有统计学意义(P<0.05)。结论丹参多酚酸盐对心绞痛患者的使用能明显降低心绞痛发作,降低炎症水平,改善心绞痛患者的临床症状,从而降低心血管事件的发生风险。 展开更多
关键词 丹参多酚酸盐 心绞痛 细胞间黏附因子-1 C反应蛋白 淀粉样蛋白A
在线阅读 下载PDF
参苓白术散加减辅助治疗难治性肾病综合征脾肾阳虚证疗效及对血清SAA和IL-6水平影响
16
作者 刘茜 孙涛 刘彬 《辽宁中医药大学学报》 CAS 2019年第5期191-194,共4页
目的:探讨参苓白术散加减辅助治疗难治性肾病综合征(RNS)脾肾阳虚证的疗效及对血清淀粉样蛋白A(SAA)和白细胞介素(IL)-6水平的影响。方法:将本院就诊的RNS患者58例按数字表法随机分为对照组(29例)和治疗组(29例)。两组给予常规干预措施... 目的:探讨参苓白术散加减辅助治疗难治性肾病综合征(RNS)脾肾阳虚证的疗效及对血清淀粉样蛋白A(SAA)和白细胞介素(IL)-6水平的影响。方法:将本院就诊的RNS患者58例按数字表法随机分为对照组(29例)和治疗组(29例)。两组给予常规干预措施和强的松。对照组采取环磷酰胺静滴,10 mg·kg-1·d-1,每周2次。治疗组在对照组基础上采取参苓白术散加减治疗,1剂/d,2次/d。两组患者连续治疗12周。比较两组脾肾阳虚证症状评分和临床疗效。采取生化分析仪检测两组24 h尿蛋白定量、血浆白蛋白、总胆固醇、甘油三酯水平。检测两组血清SAA和IL-6水平。结果:治疗后,治疗组脾肾阳虚证症状评分显著低于对照组(P<0.01)。治疗组治疗后24 h尿蛋白定量、甘油三酯、总胆固醇及症状评分明显少于对照组,血浆白蛋白显著高于对照组(P<0.01)。治疗组总有效率为93.10%,明显优于对照组为65.52%(P<0.05)。治疗组治疗后血清SAA和IL-6水平显著少于对照组(P<0.01)。结论:参苓白术散加减辅助治疗RNS脾肾阳虚证可促进症状体征改善,提高临床疗效,调节患者体内SAA和IL-6水平可能与其疗效有关。 展开更多
关键词 参苓白术散 难治性肾病综合征 脾肾阳虚证 淀粉样蛋白A 白细胞介素-6
tau蛋白和β-淀粉样蛋白在阿尔茨海默病病理过程中作用及自噬机制的研究概况 预览
17
作者 乔蕾 孙寅轶 曲忠森 《中华诊断学电子杂志》 2019年第2期94-97,共4页
阿尔茨海默病(AD)是引起老年痴呆最常见的一种神经退行性疾病。tau蛋白和β-淀粉样蛋白(Aβ)(1-42)的错误折叠和积聚引起进行性神经元丢失。目前观点认为,AD主要的生物学标志tau蛋白和Aβ能够反映其病理特征,即反映神经元外神经元纤维... 阿尔茨海默病(AD)是引起老年痴呆最常见的一种神经退行性疾病。tau蛋白和β-淀粉样蛋白(Aβ)(1-42)的错误折叠和积聚引起进行性神经元丢失。目前观点认为,AD主要的生物学标志tau蛋白和Aβ能够反映其病理特征,即反映神经元外神经元纤维缠结和神经元内老年斑的沉积。自噬是一种高度保守的溶酶体依赖性的降解程序,可以为机体供能、清理代谢废物、平衡免疫炎症反应等。笔者概述tau蛋白和Aβ在AD病理过程中的作用及自噬机制的研究现状。 展开更多
关键词 阿尔茨海默病 TAU蛋白质类 淀粉样蛋白 病理 自噬
在线阅读 下载PDF
PET和PET/MRI在阿尔茨海默病中的应用 预览
18
作者 刘思睿 有慧 冯逢(审校) 《国际医学放射学杂志》 北大核心 2019年第1期62-65,共4页
阿尔茨海默病(AD)是老年痴呆最常见的原因,AD的早期诊断有利于改善病人预后。PET和PET/MRI技术在AD的早期诊断和病情评估方面有重要的临床应用价值。现就18F-FDG PET、淀粉样蛋白PET、Tau PET以及PET/MRI在AD方面的发展现状、优势和局限... 阿尔茨海默病(AD)是老年痴呆最常见的原因,AD的早期诊断有利于改善病人预后。PET和PET/MRI技术在AD的早期诊断和病情评估方面有重要的临床应用价值。现就18F-FDG PET、淀粉样蛋白PET、Tau PET以及PET/MRI在AD方面的发展现状、优势和局限性,以及发展趋势的预测进行综述。 展开更多
关键词 阿尔茨海默病 正电子发射体层成像 18F-FDG 淀粉样蛋白 磁共振成像
在线阅读 下载PDF
In situ AFM investigation of dual-mode self-assembling peptide 预览
19
作者 Yue-Xian Bao Ming Yuan +4 位作者 Qiqige Du Yu-Bo Li Jing-Yu Gao Abdul Jamil Khan Feng Zhang 《核技术:英文版》 SCIE CAS CSCD 2019年第8期1-11,共11页
Nanostructures/patterns formed by biomolecules can produce different physicochemical properties in terms of hydrophobicity, zeta-potential, color, etc., which play paramount roles in life. Peptides, as the main bio-bu... Nanostructures/patterns formed by biomolecules can produce different physicochemical properties in terms of hydrophobicity, zeta-potential, color, etc., which play paramount roles in life. Peptides, as the main bio-building blocks, can form nanostructures with different functions,either in solutions or on interfaces. Previously, we synthesized a short peptide with the inspiration of an Alzheimer’s disease-related peptide: amyloid β peptide(A-p),namely GAV-9, which can epitaxially self-assemble into regular nanofilaments on liquid-solid interfaces, and it was found that both the hydrophobicity and charge state of the interfaces can significantly influence its assembling behavior. It was also reported that another A-β-containing dipeptide, FF,can self-assemble into nanostructures in solutions. Owing to the close relationship between these two short peptides, it is interesting to conjugate them into a de novo peptide with two separated structural domains and study its self-assembling behavior. To this end, herein we have synthesized the GAV-FF peptide with a sequence of NH2-VGGAVVAGVFF-CONH2 and verified its selfassembling property using the in situ liquid-phase atomic force microscopy. The results show that the GAV-FF peptide can self-assemble into nanofilaments both in solutions and on aqueous-solid interfaces, but with different morphologies. The FF domain accelerates the template-assisted self-assembling(TASA) process of the GAV domain, which in return enhances the solubility of FF in aqueous solutions and further participates in the fibrillization of FF. The current results could help deepen the understanding of the aggregation mechanism of diseaserelated peptides and could also shed light on the strategies to create artificial bio-functional nanostructures/patterns,which hold a significant potential for biomedical applications. 展开更多
关键词 AMYLOID PEPTIDE Nanofilament SELFASSEMBLY Structural domain Atomic force MICROSCOPY
在线阅读 下载PDF
血清YKL-40、RANTES、SSA水平和帕金森病患者MoCA评分的关系
20
作者 冯阳 唐自强 杨莉 《卒中与神经疾病》 2019年第5期595-598,共4页
目的探讨血清人软骨糖蛋白39(YKL-40)、活化T细胞趋化因子(RANTES)、淀粉样蛋白A(SSA)水平和帕金森病患者MoCA评分的关系。方法选择2017年1月-2018年11月本院接诊的80例帕金森病患者作为观察组,并选择同期于本院接受体检的健康体检者60... 目的探讨血清人软骨糖蛋白39(YKL-40)、活化T细胞趋化因子(RANTES)、淀粉样蛋白A(SSA)水平和帕金森病患者MoCA评分的关系。方法选择2017年1月-2018年11月本院接诊的80例帕金森病患者作为观察组,并选择同期于本院接受体检的健康体检者60例作为对照组,比较2组血清YKL-40、RANTES、SSA水平、蒙特利尔认知评估量表(MoCA)评分,记录观察组帕金森病统一评分量表(UPDRS)评分,分析血清YKL-40、RANTES、SSA水平和MoCA评分、UPDRS评分的相关性。结果观察组血清YKL-40、RANTES、SSA水平均明显比对照组高,MoCA评分明显低于对照组(P<0.05);经Spearman相关分析显示,血清YKL-40、RANTES、SSA水平和UPDRSⅠ评分均无明显相关性(P>0.05);血清YKL-40、RANTES、SSA水平和MoCA评分均呈负相关(r=-0.743,-0.812,-0.786,P<0.05),和UPDRSⅡ(r=0.832,0.673,0.753)、UPDRSⅢ(r=0.698,0.739,0.791)评分均呈正相关(P<0.05)。结论血清YKL-40、RANTES、SSA水平在帕金森病患者中呈明显升高趋势,且和患者MoCA评分具有密切联系,也为早期发现、防治疾病提供了一定理论基础。 展开更多
关键词 帕金森病 蒙特利尔认知评估量表评分 人软骨糖蛋白39 活化T细胞趋化因子 淀粉样蛋白A
上一页 1 2 206 下一页 到第
使用帮助 返回顶部 意见反馈