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Mechanical force drives the polarization and orientation of cells 预览
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作者 Shijie He Xiaomeng Li Baohua Ji 《力学学报:英文版》 SCIE EI CAS CSCD 2019年第2期275-288,共14页
Collective cells are organized to form specific patterns which play important roles in various physiological and pathological processes, such as tissue morphogenesis, wound healing, and cancer invasion. Compared to si... Collective cells are organized to form specific patterns which play important roles in various physiological and pathological processes, such as tissue morphogenesis, wound healing, and cancer invasion. Compared to single cell behaviors, which has been intensively studied from many aspects (cell migration, adhesion, polarization, proliferation, etc.) and at various scales (molecular, subcellular, and cellular), the multiple cell behaviors are relatively less understood, particularly in a quantitative manner. In this paper, we will present our recent studies of collective polarization and orientation of multiple cells through both experimental measurement and theoretical modeling, including those cell behaviors on/in 2D and 3D substrate/tissue. We find that the collective cell behaviors, including polarization, alignment and migration are closely related to local stress states in cell layer or tissue, which demonstrate the crucial roles of mechanical forces in the living organisms. Specifically, the cells prefer to polarize and align along the maximum principal stress in the cell layer, and the aspect ratio of cell increases with the in-plane maximum shear stress, suggesting that the maximum shear stress is the underlying driving force of cell polarization and orientation. This theory of stress-driven cell behaviors of polarization and orientation provides a new perspective for understanding cell behaviors in living organisms and the guideline for tissue engineering in biomedical applications. 展开更多
关键词 COLLECTIVE CELL behaviors CELL POLARIZATION CELL ALIGNMENT Quantification CELL MECHANOSENSING
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Truth lies below: A case report and literature review of typical appearing polyps yet with an atypical diagnosis 预览
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作者 Aaron Fisher Edward Yousif Marc Piper 《世界胃肠内镜杂志:英文版(电子版)》 2019年第1期54-59,共6页
BACKGROUND Enteropathy associated T-cell lymphoma (EATL) is a rare form of peripheral Tcell lymphoma and makes up less than 5% of gastrointestinal lymphomas. EATL can be divided into type 1 which is associated with ce... BACKGROUND Enteropathy associated T-cell lymphoma (EATL) is a rare form of peripheral Tcell lymphoma and makes up less than 5% of gastrointestinal lymphomas. EATL can be divided into type 1 which is associated with celiac disease, and monomorphic epitheliotropic intestinal T-cell lymphoma (MEITL), formally type 2, which is not associated with celiac disease. CASE SUMMARY We present a 60-year-old African American female, without celiac disease, who presented with abdominal pain, diarrhea, and 30 lb. weight loss over a 3 month period. She was subsequently diagnosed with EATL throughout her entire gastrointestinal tract. She is currently undergoing chemotherapy with EOCH (Etoposide, Oncovin, Cyclophosphamide, and Hydroxydaunorubicin). EATL is most common in the Asian and Hispanic population yet the incidence in African Americans is uncertain and emphasizes the rarity of this case. A literature review was included to further emphasize similarities and differences between our case and previously reported cases of MEITL. CONCLUSION The patient was diagnosed with EATL, immunochemical testing was not conclusive for MEITL however was suggestive of the disease. 展开更多
关键词 ENTEROPATHY associated T-CELL LYMPHOMA Monomorphic epitheliotropic intestinal T-CELL LYMPHOMA Peripheral T-CELL LYMPHOMA Gastrointestinal LYMPHOMA Endoscopy Case report LITERATURE review
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Donor-Derived CD19-Targeted T Cell Infusion Eliminates B Cell Acute Lymphoblastic Leukemia Minimal Residual Disease with No Response to Donor Lymphocytes after Allogeneic Hematopoietic Stem Cell Transplantation 预览
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作者 Yifei Cheng Yuhong Chen +11 位作者 Chenhua Yan Yu Wang Xiangyu Zhao Yao Chen Wei Han Lanping Xu Xiaohui Zhang Kaiyan Liu Shasha Wang Lungji Chang Lei Xiao Xiaojun Huang 《工程(英文)》 2019年第1期150-155,共6页
Leukemia relapse is still the leading cause of treatment failure after allogeneic hematopoietic stem cell transplantation (allo-HSCT) for B cell acute lymphoblastic leukemia (B-ALL). Relapsed patients with BALL after ... Leukemia relapse is still the leading cause of treatment failure after allogeneic hematopoietic stem cell transplantation (allo-HSCT) for B cell acute lymphoblastic leukemia (B-ALL). Relapsed patients with BALL after allo-HSCT have a very short median survival. Minimal residual disease (MRD) is predictive of forthcoming hematological relapse after hematopoietic stem cell transplantation (HSCT);furthermore, eliminating MRD effectively prevents relapse. Donor lymphoblastic infusion (DLI) is the main established approach to treat B-ALL with MRD after allo-HSCT. However, about one-third of patients with MRD are non-responsive to DLI and their prognosis worsens. Although donor-derived cluster of differentiation (CD)19-directed chimeric antigen receptor-modified (CAR) T cells (CART19s) can potentially cure leukemia, the efficiency and safety of infusions with these cells have not yet been investigated in patients with MRD after HSCT. Between September 2014 and February 2018, six patients each received one or more infusions of CART19s from HSCT donors. Five (83.33%) achieved MRD-negative remission, and one case was not responsive to the administration of CAR T cells. Three of the six patients are currently alive without leukemia. No patient developed acute graft-versus-host disease (aGVHD), and no patient died of cytokine release syndrome. Donor-derived CAR T cell infusions seem to be an effective and safe intervention for patients with MRD in B-ALL after allo-HSCT and for those who were not responsive to DLI. 展开更多
关键词 Donor-derived CD19-targeted T CELL INFUSION Hematopoietic stem CELL transplantation B CELL acute lymphoblastic leukemia Minimal residual disease
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miR-34c inhibits proliferation and enhances apoptosis in immature porcine Sertoli cells by targeting the SMAD7 gene 预览
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作者 RAN Mao-liang WENG Bo +4 位作者 CAO Rong PENG Fu-zhi LUO Hui GAO Hu CHEN Bin 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第2期449-459,共11页
MicroRNAs(miRNAs) are implicated in swine spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation. Recent studies indicated that miR-34 c is indispensable in the late steps of sperm... MicroRNAs(miRNAs) are implicated in swine spermatogenesis via their regulations of cell proliferation, apoptosis, and differentiation. Recent studies indicated that miR-34 c is indispensable in the late steps of spermatogenesis. However, whether miR-34 c plays similar important roles in immature porcine Sertoli cells remain unknown. In the present study, we conducted two experiments using a completely randomised design to study the function roles of miR-34 c. The results from experiment I demonstrated that the relative expression level of miR-34 c in swine testicular tissues increased(P=0.0017) quadratically with increasing age, while the relative expression level of SMAD family member 7(SMAD7) decreased(P=0.0009) with curve. Furthermore, miR-34 c expression levels showed a significant negative correlation(P=0.013) with SMAD7 gene expression levels. The results from experiment II indicated that miR-34 c directly targets the SMAD7 gene using a luciferase reporter assay, and suppresses(P<0.05) SMAD7 mRNA and protein expressions in immature porcine Sertoli cells. Overexpression of miR-34 c inhibited(P<0.05) proliferation and enhanced(P<0.05) apoptosis in the immature porcine Sertoli cells, which was supported by the results from the Cell Counting Kit-8(CCK-8) assay, the 5-Ethynyl-2′-deoxyuridine(EdU) assay, and the Annexin V-FITC/PI staining assay. Furthermore, knockdown of SMAD7 via small interfering RNA(siR NA) gave a similar result. It is concluded that miR-34 c inhibits proliferation and enhances apoptosis in immature porcine Sertoli cells by targeting the SMAD7 gene. 展开更多
关键词 IMMATURE PORCINE Sertoli CELL CELL PROLIFERATION APOPTOSIS SMAD7 miR-34c
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A novel mouse model of adult T-cell leukemia cell invasion into the spinal cord 预览
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作者 Takeo Ohsugi Shuhei Tanaka +5 位作者 Keigo Iwasaki Yusuke Nagano Tomohiro Kozako Kazuya Matsuda Takuya Hirose Kazushige Takehana 《动物模型与实验医学(英文)》 CSCD 2019年第1期64-67,共4页
Adult T-cell leukemia(ATL)is a mature T-cell malignancy caused by human T-cell leukemia virus type I infection,and 10%-25%of patients show central nervous system(CNS)involvement.CNS involvement significantly reduces s... Adult T-cell leukemia(ATL)is a mature T-cell malignancy caused by human T-cell leukemia virus type I infection,and 10%-25%of patients show central nervous system(CNS)involvement.CNS involvement significantly reduces survival and there are no effective treatments for CNS involvement.Therefore,an appropriate animal model is required to evaluate the inhibitory effects of novel drugs on the progression of ATL with CNS involvement.Here,we established a mouse model of ATL with CNS involvement using NOD.Cg-Prkdc scid Il2rg tm1Wjl/SzJ mice inoculated with ATL cells intramuscularly in the postauricular region,and these mice showed paraparesis.Of the 10 mice inoculated with ATL cells intramuscularly(I.M.)at 5 weeks of age,8(80%)showed paraparesis,whereas none of the 10 mice inoculated with ATL cells subcutaneously(S.C.)showed paraparesis.In the I.M.group,PCR detected HTLV-1-specific genes in the thoracic and lumbar vertebrae;however,in the S.C.group,the vertebrae were negative for HTLV-1 genes.Histological analysis revealed a particularly high incidence of tumors,characterized by accumulation of the injected cells,in the thoracic vertebrae of mice in the I.M.group.Tumor cell infiltration was relatively high in the bone marrow.Spinal cord compression caused by invasion of the tumor mass outside the pia mater was observed in the thoracic vertebrae of the spinal cord.In conclusion,we have reported a mouse model of tumor growth with paraparesis that may be used to assess novel therapeutic agents for ATL with CNS involvement. 展开更多
关键词 adult T-CELL leukemia(ATL) central nervous system(CNS) human T-CELL LEUKEMIA virus type I(HTLV-1) MICE NOD.Cg-Prkdc SCID Il2rg tm1Wjl/SzJ MICE
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Exogenous neural stem cell transplantation for cerebral ischemia 预览
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作者 Ling-Yi Liao Benson Wui-Man Lau +1 位作者 Dalinda Isabel Sánchez-Vida?a Qiang Gao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1129-1137,共9页
Cerebral ischemic injury is the main manifestation of stroke,and its incidence in stroke patients is 70–80%.Although ischemic stroke can be treated with tissue-type plasminogen activator,its time window of effectiven... Cerebral ischemic injury is the main manifestation of stroke,and its incidence in stroke patients is 70–80%.Although ischemic stroke can be treated with tissue-type plasminogen activator,its time window of effectiveness is narrow.Therefore,the incidence of paralysis,hypoesthesia,aphasia,dysphagia,and cognitive impairment caused by cerebral ischemia is high.Nerve tissue regeneration can promote the recovery of the aforementioned dysfunction.Neural stem cells can participate in the reconstruction of the damaged nervous system and promote the recovery of nervous function during self-repair of damaged brain tissue.Neural stem cell transplantation for ischemic stroke has been a hot topic for more than 10 years.This review discusses the treatment of ischemic stroke with neural stem cells,as well as the mechanisms of their involvement in stroke treatment. 展开更多
关键词 nerve REGENERATION STEM CELL therapy NEURAL STEM cells CELL transplantation ischemic stroke cerebral ischemia NEUROPLASTICITY functional recovery NEURAL REGENERATION
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Factors in?uencing the somatic cell nuclear transfer ef?ciency in pigs
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作者 Yong JIN Manling ZHANG +15 位作者 Xinrong JU Shuang LIANG Qiang XIONG Lihua ZHAO Xiaowei NIE Daorong HOU Qiang LIU Junzheng WANG Chenyu WANG Xiaokang LI Lining ZHANG Xiaorui LIU Ying WANG Haiyuan YANG Yifan DAI Rongfeng LI 《农业科学与工程前沿:英文版》 2019年第1期73-80,共8页
Using a data set from our laboratory, we assessed the effects of several factors on pig cloning ef?ciency. The results demonstrated that cells at high con?uence( > 90%) used as donor cell resulted in higher pregnan... Using a data set from our laboratory, we assessed the effects of several factors on pig cloning ef?ciency. The results demonstrated that cells at high con?uence( > 90%) used as donor cell resulted in higher pregnancy rate, delivery rate and overall cloning ef?ciency(number of live offspring born per reconstructed embryo transferred to recipients) compared with the cells at 60% to79% con?uence and 80% to 89% con?uence. Cells with four, ?ve and six passages compromised the pregnancy and delivery rates compared with ?rst passage cells. The number of blastocysts transferred by somatic cell nuclear transfer(SCNT) did not signi?cantly affect the cloning ef?ciency, but transfer of blastocyst derived from in vitro culture 5 d after SCNT achieved a signi?cantly higher pregnancy rate compared with one to two cell SCNT embryos from overnight culture. The highest pregnancy rate, delivery rate and the largest litter size were obtained when Bama Miniature pig ?broblasts were used as donor cells and Landrace/Yorkshire hybrid gilts were used as recipients. Recipients treated with chemicals for estrus synchronization had higher pregnancy rates compared with untreated recipients. Our data might be helpful for improving SCNT ef?ciency in pigs. 展开更多
关键词 BLASTOCYST donor CELL ESTRUS synchronization pregnancy rate PIG cloning SOMATIC CELL nuclear transfer
Effect of Yanggan Jiedu Sanjie formula on human hepatocellular carcinoma Bel-7402 cells
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作者 Hu Bing Zhang Tong +5 位作者 An Hongmei Zheng Jialu Yan Xia Huang Xiaowei Tian Jianhui Li Miao 《中医杂志:英文版》 SCIE CAS CSCD 2019年第1期26-33,共8页
OBJECTIVE: To observe the effect of Yanggan Jiedu Sanjie(YGJDSJ) formula on human hepatocellular carcinoma Bel-7402 cells.METHODS: Bel-7402 cells were treated with YGJDSJ. Cell proliferation was detected by cell count... OBJECTIVE: To observe the effect of Yanggan Jiedu Sanjie(YGJDSJ) formula on human hepatocellular carcinoma Bel-7402 cells.METHODS: Bel-7402 cells were treated with YGJDSJ. Cell proliferation was detected by cell counting kit-8 assay. Cell apoptosis was identified by Hoechst 33258 staining and flow cytometric analysis. Cell cycle distribution was quantified by flow cytometric analysis. Caspase activities were measured by commercial kit. Cell senescence was detected by senescence-associated β-galactosidase(SA-β-gal)staining. Protein expression and phosphorylation were identified by Western blot. Protein expression was knocked-down by siRNA.RESULTS: YGJDSJ inhibited proliferation of Bel-7402 cells in a dose-and time-dependent manner.YGJDSJ induced apoptosis and activated caspase-3, 8, and 9 in Bel-7402 cells. YGJDSJ-induced apoptosis was completely abrogated by a pan caspase inhibitor, Z-VAD-FMK. YGJDSJ also induced cell senescence, up-regulated cyclin-dependent kinase inhibitor 1 a(CDKN1 a) and CDKN2 a expression and down-regulated retinoblastoma protein(RB) phosphorylation in Bel-7402 cells. Specific knockdown of CDKN1 a and CDKN2 a significantly reduced YGJDSJ-induce cell senescence in Bel-7402 cells.CONCLUSION: YGJDSJ inhibited cell proliferation,induced caspase-dependent apoptosis and CDKN1 a/CDKN2 a-RB signalling mediated cell senescence in Bel-7402 cells. Our findings suggest that YGJDSJ might be potential for hepatocellular carcinoma treatment. 展开更多
关键词 Carcinoma hepatocellular Chinese herbal FORMULA Apoptosis CELL SENESCENCE CELL cycle Cyclin-dependent KINASE INHIBITOR p21 Cyclindependent KINASE INHIBITOR p16 RETINOBLASTOMA protein
Synergistic effects of combined therapy of curcumin and Fructus Ligustri Lucidi for treatment of osteoporosis: cellular and molecular evidence of enhanced bone formation
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作者 Syed Nasir Abbas Bukhari Fahad Hussain +1 位作者 Hnin Ei Thu Zahid Hussain 《结合医学学报:英文版》 CAS CSCD 2019年第1期38-45,共8页
OBJECTIVE: The present study explored the effects of the combined herbal therapy consisting of curcumin (CUR) and Fructus Ligustri Lucidi (FLL) on aspects of bone regeneration. METHODS: Prior to analyzing the ability ... OBJECTIVE: The present study explored the effects of the combined herbal therapy consisting of curcumin (CUR) and Fructus Ligustri Lucidi (FLL) on aspects of bone regeneration. METHODS: Prior to analyzing the ability of this novel combined herbal therapy to promote aspects of bone regeneration, its cytotoxicity was determined using MC3T3-E1 cells (pre-osteoblast model). Cell proliferation was evaluated using phase-contrast microscopy and cell differentiation was estimated using alkaline phosphatase activity. The effect of the combined herbal therapy (CUR + FLL) was also assessed in terms of mineralization in the extracellular matrix (ECM) of cultured cells. Further, to explore the molecular mechanisms of bone formation, time-dependent expression of bone-regulating protein biomarkers was also evaluated. RESULTS: Combined herbal therapy (CUR + FLL) significantly upregulated the viability, proliferation and differentiation of MC3T3-E1 cells compared to the monotherapy of CUR or FLL. The magnitude of ECM mineralization (calcium deposition) was also higher in MC3T3-E1 cells treated with combined therapy. The time-dependent expression of bone-forming protein biomarkers revealed that the tendency of expression of these bone-regulating proteins was remarkably higher in cells treated with combined therapy.CONCLUSION: The co-administration of CUR and FLL had superior promotion of elements of bone regeneration in cultured cells, thus could be a promising alternative herbal therapy for the management of bone erosive disorders such as osteoporosis. 展开更多
关键词 OSTEOPOROSIS CURCUMIN Fructus Ligustri Lucidi Combined HERBAL therapy CELL proliferation CELL DIFFERENTIATION BONE formation
Extreme hyperbilirubinemia: An indicator of morbidity and mortality in sickle cell disease 预览
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作者 John Paul Haydek Cesar Taborda +4 位作者 Rushikesh Shah Preeti A Reshamwala Morgan L McLemore Fuad El Rassi Saurabh Chawla 《世界肝病学杂志:英文版(电子版)》 2019年第3期287-293,共7页
BACKGROUND Sickle cell disease (SCD) is a disorder that results in increased hospitalizations and higher mortality. Advances in management have resulted in increases in life expectancy and led to increasing awareness ... BACKGROUND Sickle cell disease (SCD) is a disorder that results in increased hospitalizations and higher mortality. Advances in management have resulted in increases in life expectancy and led to increasing awareness of sickle cell hepatopathy (SCH). However, its impact in patients on the natural history and outcomes of SCD is not known. Our study aims to describe the prevalence of extreme hyperbilirubinemia (EH), one form of SCH, its effect on morbidity and mortality, and correlations between sickle cell genotype and SCH type. We hypothesize that EH is associated with higher morbidity and mortality. AIM To investigate the effects of EH on morbidity and mortality among patients with SCD. METHODS This retrospective cohort study was performed using a database of patients with SCD treated at Grady Memorial Hospital between May 2004 and January 2017. Patients with EH (defined as total bilirubin above 13.0 mg/dL) were identified. A control group was identified from the same database with patients with total serum bilirubin ≤ 5.0 mg/dL. Electronic medical records were used to extract demographic information, laboratory values, radiology results, current medications, need for transfusions and mortality data. Two samples T-test, chisquared test and Fisher's exact test were then used to compare the parameters between the two groups. RESULTS Out of the database, fifty-seven charts were found of patients with bilirubin > 13 mg/dL. Prevalence of severe SCH as defined by EH was 4.8%(57/1172). There were no demographic differences between patients with and without EH. Significant genotypic differences existed between the two groups, with hemoglobin SS SCD being much higher in the EH group (P < 0.001). Patients with severe EH had a significant elevations in alanine aminotransferase (157.0 ± 266.2 IU/L vs 19.8 ± 21.3 IU/L, P < 0.001), aspartate aminotransferase (256.5 ± 485.9 U/L vs 28.2 ± 14.7 U/L, P < 0.001) and alkaline phosphatase (218.0 ± 176.2 IU/L vs 85.9 ± 68.4 IU/L, P < 0.001). Patients with EH had significantly 展开更多
关键词 SICKLE CELL disease SICKLE CELL HEPATOPATHY Liver diseases EXTREME HYPERBILIRUBINEMIA MORTALITY
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Cell-imprinted polydimethylsiloxane for the selective cell adhesion
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作者 Lukuan Liu Kaiguang Yang +4 位作者 Zhongpeng Dai Zhen Liang Lihua Zhang Xiaojun Peng Yukui Zhang 《中国化学快报:英文版》 SCIE CAS CSCD 2019年第3期672-675,共4页
Cell adhesion is the basis for some cell isolation methods, and is influenced by both of the biochemical and topographic characteristics of the substrates. Herein, based on cell imprinting and click chemistry,we have ... Cell adhesion is the basis for some cell isolation methods, and is influenced by both of the biochemical and topographic characteristics of the substrates. Herein, based on cell imprinting and click chemistry,we have developed a cell-imprinted polydimethylsiloxane(PDMS) with aptamer functionalization(APTCIS). The atom force microscopic analysis results showed that the hierarchical structure matching well with the target cells is successfully introduced on the surface of the APT-CIS. By using the synergistic effects of hierarchical structure and aptamer affinity, the APT-CIS was successfully used for the selective cell adhesion, and 93.9%± 0.8% of the captured cells could then be released. Thus, the APT-CIS holds promise in selective cell isolation and sorting fields. 展开更多
关键词 CELL adhesion APTAMER CELL IMPRINTING Hierarchical structures CLICK chemistry
Memory stem T cells generated by Wnt signaling from blood of human renal clear cell carcinoma patients 预览
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作者 Cihui Yan Jingjing Chang +6 位作者 Xinmiao Song Fan Yan Wenwen Yu Yang An Feng Wei Lili Yang Xiubao Ren 《癌症生物学与医学:英文版》 CAS CSCD 2019年第1期109-120,共12页
Objective: Memory stem T cells(Tscm) have attracted attention because of their enhanced self-renewal, multipotent capacity, and anti-tumor capacities. However, little is known about Tscm in patients with renal clear c... Objective: Memory stem T cells(Tscm) have attracted attention because of their enhanced self-renewal, multipotent capacity, and anti-tumor capacities. However, little is known about Tscm in patients with renal clear cell carcinoma(RCC) and the role of Wnt signaling in these cells. We evaluated Tscm from RCC patients concerning their activation of Wnt signaling in vitro and explored the mechanism of preferential survival.Methods: Flow cytometry identified surface markers and cytokines produced from accumulated Tscm in the presence of the glycogen synthase kinase beta inhibitor TWS119. Apoptosis was evaluated after induction using tumor necrosis factor-alpha.Immunofluorescence and Western blot analyses were used to investigate the activation of the nuclear factor-kappa B(NF-ΚB)pathway.Results: RCC patients had a similar percentage of CD4~+ and CD8~+ Tscm as healthy donors. Activation of Wnt signaling by TWS119 resulted in the accumulation of Tscm in activated T cells, but reversal of differentiated T cells to Tscm was not achieved.Preferential survival of Tscm was associated with increased anti-apoptotic ability mediated downstream of the NF-ΚB activation pathway.Conclusions: The finding that Tscm can accumulate by Wnt signaling in vitro in blood from RCC patients will help in devising new cancer therapy strategies of Tscm-based adoptive immunotherapy, such as dendritic cell-stimulated Tscm, and T cell receptor or chimeric antigen receptor-engineered Tscm. 展开更多
关键词 MEMORY STEM T CELL TWS119 Wnt signaling apoptosis RENAL CLEAR CELL carcinoma
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Potassium bisperoxo(1,10-phenanthroline)oxovanadate suppresses proliferation of hippocampal neuronal cell lines by increasing DNA methyltransferases 预览
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作者 Xiao-Li Tian Shu-Yuan Jiang +7 位作者 Xiao-Lu Zhang Jie Yang Jun-He Cui Xiao-Lei Liu Ke-Rui Gong Shao-Chun Yan Chun-Yang Zhang Guo Shao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期826-833,共8页
Bisperoxo(1,10-phenanthroline)oxovanadate(BpV)can reportedly block the cell cycle.The present study examined whether BpV alters gene expression by affecting DNA methyltransferases(DNMTs),which would impact the cell cy... Bisperoxo(1,10-phenanthroline)oxovanadate(BpV)can reportedly block the cell cycle.The present study examined whether BpV alters gene expression by affecting DNA methyltransferases(DNMTs),which would impact the cell cycle.Immortalized mouse hippocampal neuronal precursor cells(HT22)were treated with 0.3 or 3μM BpV.Proliferation,morphology,and viability of HT22 cells were detected with an IncuCyte real-time video imaging system or inverted microscope and 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium,respectively.mRNA and protein expression of DNMTs and p21 in HT22 cells was detected by real-time polymerase chain reaction and immunoblotting,respectively.In addition,DNMT activity was measured with an enzyme-linked immunosorbent assay.Effects of BpV on the cell cycle were analyzed using flow cytometry.Results demonstrated that treatment with 0.3μM BpV did not affect cell proliferation,morphology,or viability;however,treatment with 3μM BpV decreased cell viability,increased expression of both DNMT3B mRNA and protein,and inhibited the proliferation of HT22 cells;and 3μM BpV also blocked the cell cycle and increased expression of the regulatory factor p21 by increasing DNMT expression in mouse hippocampal neurons. 展开更多
关键词 nerve REGENERATION hippocampal neurons POTASSIUM bisperoxo(1 10-phenanthroline)oxovanadate DNA METHYLTRANSFERASE p21 HT22 CELL CELL cycle immunoblotting DNA methylation neural REGENERATION
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Regenerative potential of mouse embryonic stem cell-derived PDGFRα+cardiac lineage committed cells in infarcted myocardium 预览
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作者 Seon Pyo Hong Sukhyun Song +5 位作者 Seungjoo Lee Hyeonju Jo Hyoung Kyu Kim Jin Han Jae-Hyeong Park Sung Woo Cho 《世界干细胞杂志:英文版(电子版)》 2019年第1期44-54,共11页
BACKGROUND Pluripotent stem cell-derived cardiomyocytes(CMs)have become one of the most attractive cellular resources for cell-based therapy to rescue damaged cardiac tissue.AIM We investigated the regenerative potent... BACKGROUND Pluripotent stem cell-derived cardiomyocytes(CMs)have become one of the most attractive cellular resources for cell-based therapy to rescue damaged cardiac tissue.AIM We investigated the regenerative potential of mouse embryonic stem cell(ESC)-derived platelet-derived growth factor receptor-α(PDGFRα)^+cardiac lineagecommitted cells(CLCs),which have a proliferative capacity but are in a morphologically and functionally immature state compared with differentiated CMs.METHODS We induced mouse ESCs into PDGFRα^+CLCs andαMHC^+CMs using a combination of the small molecule cyclosporin A,the rho-associated coiled-coil kinase inhibitor Y27632,the antioxidant Trolox,and the ALK5 inhibitor EW7197.We implanted PDGFRα^+CLCs and differentiatedαMHC+^CMs into a myocardial infarction(MI)murine model and performed functional analysis using transthoracic echocardiography(TTE)and histologic analysis.RESULTS Compared with the untreated MI hearts,the anterior and septal regional wall motion and systolic functional parameters were notably and similarly improved in the MI hearts implanted with PDGFRα^+CLCs andαMHC+CMs based on TTE.In histologic analysis,the untreated MI hearts contained a thinner ventricular wall than did the controls,while the ventricular walls of MI hearts implanted with PDGFRα^+CLCs andαMHC^+CMs were similarly thicker compared with that of the untreated MI hearts.Furthermore,implanted PDGFRα+CLCs aligned and integrated with host CMs and were mostly differentiated intoα-actinin^+CMs,and they did not convert into CD31^+end othelial cells orαSMA^+mural cells.CONCLUSION PDGFRα^+CLCs from mouse ESCs exhibiting proliferative capacity showed a regenerative effect in infarcted myocardium.Therefore,mouse ESC-derived PDGFRα^+CLCs may represent a potential cellular resource for cardiac regeneration. 展开更多
关键词 PLURIPOTENT STEM CELL Embryonic STEM CELL CARDIOMYOCYTE Myocardial infarction Regeneration
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PLZF^posc-KIT^pos-delineated A1-A4-differentiating spermatogonia by subset and stage detection upon Bouin fixation
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作者 Rui-Ling Tang Li-Qing Fan 《亚洲男性学杂志:英文版》 SCIE CAS CSCD 2019年第3期309-318,共10页
While hallmarks of rodent spermatogonia stem cell biomarkers' heterogeneity have recently been identified, their stage and subset distributions remain unclear. Furthermore, it is currently difficult to accurately ... While hallmarks of rodent spermatogonia stem cell biomarkers' heterogeneity have recently been identified, their stage and subset distributions remain unclear. Furthermore, it is currently difficult to accurately identify subset-specific SSC marker distributions due to the poor nuclear morphological characteristics associated with fixation in 4% paraformaldehyde. In the present study, testicular cross-sections and whole-mount samples were Bouin fixed to optimize nuclear resolution and visualized by immunohistochemistry (IHC) and immunofluorescence (IF). The results identified an expression pattern of PLZFhighc-KITpos in A1 spermatogonia, while A2–A4-differentiating spermatogonia were PLZFlowc-KITpos. Additionally, this procedure was used to examine asymmetrically expressing GFRA1 and PLZF clones, asymmetric Apr and false clones were distinguished based on the presence or absence of TEX14, a molecular maker of intercellular bridges, despite having identical nuclear morphology and intercellular distances that were <25 μm. In conclusion, this optimized Bouin fixation procedure facilitates the accurate identification of spermatogonium subsets based on their molecular profiles and is capable of distinguishing asymmetric and false clones. Therefore, the findings presented herein will facilitate further morphological and functional analysis studies and provide further insight into spermatogonium subtypes. 展开更多
关键词 asymmetric division cellular homolog of FELINE sarcoma viral oncogene v-kit false CLONES glial CELL line-derived neurotrophic factor receptor alpha 1 PROMYELOCYTIC leukemia zinc finger SPERMATOGONIA stem CELL
Discussion of some‘knowns’and some‘unknowns’about the tumour suppressor p53
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作者 Elizabeth Lieschke Zilu Wang +1 位作者 Gemma L Kelly Andreas Strasser 《分子细胞生物学报:英文版》 SCIE CAS CSCD 2019年第3期212-223,共12页
Activation of the tumour suppressor p53 upon cellular stress can induce a number of different cellular processes?The diverse actions of these processes are critical for the protective function of p53 in preventing the... Activation of the tumour suppressor p53 upon cellular stress can induce a number of different cellular processes?The diverse actions of these processes are critical for the protective function of p53 in preventing the development of cancer.However,it is still not fully understood which process(es)activated by p53 is/are critical for tumour suppression and how this might differ depending on the type of cells undergoing neoplastic transformstion and the nature of the drivers of oncogenesis.Moreover,it is not clear why upon activation of p53 some cells undergo cell cycle arrest and senescence whereas others die by apoptosis.Here we discuss some of the cellular processes that are crucial for p53-mediated tumour suppression and the factors that could impact cell fate upon p53 activation.Finally,we describe therapies aimed either at activating wild-type p53 or at changing the behaviour of mutant p53 to unleash tumour growth suppressive processes for therapeutic benefit in malignant disease. 展开更多
关键词 P53 TUMOUR SUPPRESSION CELL DEATH CELL cycle arrest/senescenee
Ethanol exposed maturing rat cerebellar granule cells show impaired energy metabolism and increased cell death after oxygen-glucose deprivation 预览
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作者 Ana Spataru Diana Le Duc +1 位作者 Leon Zagrean Ana-Maria Zagrean 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第3期485-490,共6页
Alcohol, a widely abused drug, has deleterious effects on the immature nervous system. This study investigates the effect of chronic in vitro ethanol exposure on the metabolism of immature rat cerebellar granular cell... Alcohol, a widely abused drug, has deleterious effects on the immature nervous system. This study investigates the effect of chronic in vitro ethanol exposure on the metabolism of immature rat cerebellar granular cells (CGCs) and on their response to oxygen-glucose deprivation (OGD). Primary CGC cultures were exposed to ethanol (100 mM in culture medium) or to control ethanol-free medium starting day one in vitro (DIV1). At DIV8, the expression of ATP synthase gene ATP5g3 was quantified using real-time PCR, then cultures were exposed to 3 hours of OGD or normoxic conditions. Subsequently, cellular metabolism was assessed by a resazurin assay and by ATP level measurement. ATP5g3 expression was reduced by 12-fold (P = 0.03) and resazurin metabolism and ATP level were decreased to 74.4±4.6% and 55.5 ±6.9%, respectively after chronic ethanol treatment compared to control values (P<0.01). Additionally, after OGD exposure of ethanol-treated cultures, resazurin metabolism and ATP level were decreased to 12.7±1.0% and 9.0±2.0% from control values (P<0.01). These results suggest that chronic ethanol exposure reduces the cellular ATP level, possibly through a gene expression down-regulation mechanism, and increases the vulnerability to oxygen-glucose deprivation. Thus, interventions which improve metabolic function and sustain ATP-levels could attenuate ethanol-induced neuronal dysfunction and should be addressed in future studies. 展开更多
关键词 CELL culture chronic ETHANOL exposure oxygen-glucose DEPRIVATION CEREBELLAR granule cells toxicity gene expression CELLULAR ATP CELLULAR metabolism metabolic impairment CELL death
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Detection of cancer cells based on glycolytic-regulated surface electrical charges
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作者 Wenjun Le Bingdi Chen +2 位作者 Zheng Cui Zhongmin Liu Donglu Shi 《生物物理学报:英文版》 CSCD 2019年第1期10-18,共9页
Over the past decades, cell surface charge, although experimentally observed, has not been well understood particularly from the viewpoint of biophysics. Our recent studies have shown that all cancer cells exhib让 neg... Over the past decades, cell surface charge, although experimentally observed, has not been well understood particularly from the viewpoint of biophysics. Our recent studies have shown that all cancer cells exhib让 negative surface charges that are directly proportional to the secreted lactic acid, a unique cancer metabolic characteristic: high rate of glycolysis. We have therefore designed and developed a set of electrically-charged, fluorescent, and super-paramagnetic nanoprobes, capable of sensitive detection of cancer cells based on the surface charges. These probes are utilized to bind onto cells via electrostatic reaction for capture and magnetic separation. In this fashion, we are able to characterize cell surface charges that are regulated by different metabolic patterns, therefore effectively distinguishing the cancer cells from the normal cells. All 22 cancer cells of different organs are found to be negativelycharged therefore bound strongly by the positively-charged nanoprobes, whereas the normal cells show insignificant binding to the nanoprobes of either charge signs (positive or negative). This finding suggests that all tested cancer cells are negatively-charged and normal cells are either charge-neutral or slightly positive. For diagnosis, cancer cells can be detected, electrostatically bound, and magnetically separated in blood by charged and super-paramagnetic nanoprobes. In therapeutics, circulating cancer cells (CTCs) can be filtered and removed in a continuous fashion to reduce the risk of cancer metastasis. If successful, this new nano technology will revolutionize early cancer diagnosis and potentially enable new therapeutics in clinical settings. 展开更多
关键词 NANOTECHNOLOGY Cancer CELL CELL SURFACE charge CIRCULATING tumor cells
Plasma cell leukemia-one in a million: A case report 预览
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作者 Akriti Gupta Jain Mohammed Faisal-Uddin +3 位作者 Abdul K Khan Mohammed Wazir Qi Shen Manoucher Manoucheri 《世界临床肿瘤学杂志(英文版)》 2019年第3期161-165,共5页
BACKGROUND Plasma cell leukemia(PCL) is diagnosed by the presence of an absolute plasma cell count of > 2 × 109/L or 20% plasma cells in the peripheral blood. Because the incidence of PCL is relatively low, ou... BACKGROUND Plasma cell leukemia(PCL) is diagnosed by the presence of an absolute plasma cell count of > 2 × 109/L or 20% plasma cells in the peripheral blood. Because the incidence of PCL is relatively low, our case report study presents a rare opportunity to describe the clinical and pathological characteristics of this leukemia, as well as different modalities of treatment and outcomes of primary PCL(pPCL).CASE SUMMARY A 56-year-old male with a history of hypertension complained of pain in the left flank area which started four months prior to admission. On admission, his vital signs were stable, and physical examination was completely benign. Laboratory evaluation showed hemoglobin of 5.1 g/dL, white blood cell count of 6.6 cells per cubic millimeter with 16% atypical lymphocytes, and platelet count of 51000 per microliter. Peripheral smear showed more than 10%-15% of plasma cells(Figure1), and flow cytometry of peripheral blood confirmed PCL with 24% plasma cells CD138+. Bone marrow biopsy demonstrated 80% plasma cells(38+, 138+, 117+,10-, 19-, 20-, 56-) with 90% cellularity. The Oncology team was consulted, and VCD therapy was started. After completing therapy at 1, 4, 8, and 11 d, the patient was discharged home. The patient was being considered for a bone marrow transplant evaluation within two months of discharge.CONCLUSION PCL is a rare and aggressive form of leukemia with a poor prognosis. Multicenter studies and clinical trials should be conducted to develop accurate criteria for the initial diagnosis and prompt treatment of this disease. 展开更多
关键词 Primary PLASMA CELL LEUKEMIA Case report Rare LEUKEMIA Secondary PLASMA CELL LEUKEMIA ALLOGENIC transplantation CHEMOTHERAPY
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A Regulatory Module Controlling CA-Mediated Endosperm Cell Expansion Is Critical for Seed Germination in Arabidopsis
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作者 Rocio Sanchez-Montesino Laura Bouza-Morcillo +6 位作者 Julietta Marquez Melania Ghita Salva Duran-Nebreda Luis Gomez Michael J.Holdsworth George Bassel Luis Onate-Sanchez 《分子植物:英文版》 SCIE CAS CSCD 2019年第1期71-85,共15页
A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue.Endosperm cell separation is therefore thought to play a critical role... A key component of seed germination is the interplay of mechanical forces governing embryo growth and the surrounding restraining endosperm tissue.Endosperm cell separation is therefore thought to play a critical role in the control of this developmental transition.Here we demonstrate that in Arabidopsis thaliana seeds,endosperm cell expansion is a key component of germination.Endosperm cells expand to accommodate embryo growth prior to germination.We show that this is an actively regulated process supported by spatiotemporal control of the cell expansion gene EXPANSIN 2 (EXPA2).The NAC transcription factors NAC25 and NACIL were identified as upstream regulators of EXPA2 expression,gibbereliinmediated endosperm expansion,and seed germination.The DELLA protein RGL2 repressed activation of the EXPA2 promoter by NAC25/NACIL.Taken together,our findings uncover a key role of the GA/ DELLA-NAC25/NACIL-EXPA2 network in regulating endosperm cell expansion to control the seed-toseedling transition. 展开更多
关键词 cell expansion CELL-WALL REMODELING enzymes ENDOSPERM EXPANSIN NAC transcription factors seed GERMINATION
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