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Transplantation of adult spinal cord grafts into spinal cord transected rats improves their locomotor function
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作者 He Shen Xi Chen +3 位作者 Xing Li Ke Jia Zhifeng Xiao Jianwu Dai 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第6期725-733,共9页
Grafted embryonic central neural tissue pieces can recover function of hemisected spinal cord in neonatal rats and promote axonal growth in adults. However, spinal cord segments from adults have not been used as donor... Grafted embryonic central neural tissue pieces can recover function of hemisected spinal cord in neonatal rats and promote axonal growth in adults. However, spinal cord segments from adults have not been used as donor segments for allogeneic transplantation. Here, we utilized adult spinal cord tissue grafts(aSCGs) as donor constructs for repairing complete spinal cord injury(SCI). Moreover, to provide a favourable microenvironment for SCI treatment, a growth factor cocktail containing three growth factors(brain-derived neurotrophic factor, neurotrophin-3 and vascular endothelial growth factor), was applied to the aSCG transplants. We found that the locomotor function was significantly improved 12 weeks after transplantation of aSCGs into the spinal cord lesion site in adult rats. Transplantation of aSCGs combined with these growth factors enhanced neuron and oligodendrocyte survival and functional restoration. These encouraging results indicate that treatment of complete SCI by transplanting aSCGs, especially in the presence of growth factors, has a positive effect on motor functional recovery, and therefore could be considered as a possible therapeutic strategy for SCI. 展开更多
关键词 SPINAL CORD injury (SCI) TRANSPLANTATION ADULT SPINAL CORD GRAFTS (aSCGs) function recovery ADULT host transection
Use of ebselen as a neuroprotective agent in rat spinal cord subjected to traumatic injury 预览
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作者 Wojciech Susarczyk Edyta Olakowska +5 位作者 Magdalena Larysz-Brysz Izabella Woszczycka-Korczyńska Daria Gendosz de Carrillo Wladyslaw P.Weglarz Joanna Lewin-Kowalik Wieslaw Marcol 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1255-1261,共7页
Spinal cord injury(SCI)causes disturbances of motor skills.Free radicals have been shown to be essential for the development of spinal cord trauma.Despite some progress,until now no effective pharmacological therapies... Spinal cord injury(SCI)causes disturbances of motor skills.Free radicals have been shown to be essential for the development of spinal cord trauma.Despite some progress,until now no effective pharmacological therapies against SCI have been verified.The purpose of our experiment was to investigate the neuroprotective effects of ebselen on experimental SCI.Twenty-two rats subjected to SCI were randomly subjected to SCI with no further treatment(n=10)or intragastric administration of ebselen(10 mg/kg)immediately and 24 hours after SCI.Behavioral changes were assessed using the Basso,Beattie,and Bresnahan locomotor scale and footprint test during 12 weeks after SCI.Histopathological and immunohistochemical analyses of spinal cords and brains were performed at 12 weeks after SCI.Magnetic resonance imaging analysis of spinal cords was also performed at 12 weeks after SCI.Rats treated with ebselen presented only limited neurobehavioral progress as well as reduced spinal cord injuries compared with the control group,namely length of lesions(cysts/scars)visualized histopathologically in the spinal cord sections was less but cavity area was very similar.The same pattern was found in T2-weighted magnetic resonance images(cavities)and diffusion-weighted images(scars).The number of FluoroGold retrogradely labeled neurons in brain stem and motor cortex was several-fold higher in ebselen-treated rats than in the control group.The findings suggest that ebselen has only limited neuroprotective effects on injured spinal cord.All exprimental procedures were approved by the Local Animal Ethics Committee for Experiments on Animals in Katowice(Katowice,Poland)(approval No.19/2009). 展开更多
关键词 EBSELEN ANTIOXIDANT NEUROPROTECTION NEUROREGENERATION SPINAL CORD injury SPINAL CORD repair functional recovery
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Spinal Cord Stimulation for Pain Treatment After Spinal Cord Injury
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作者 Qian Huang Wanru Duan +6 位作者 Eellan Sivanesan Shuguang Liu Fei Yang Zhiyong Chen Neil C.Ford Xueming Chen Yun Guan 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第3期527-539,共13页
In addition to restoration of bladder, bowel, and motor functions, alleviating the accompanying debilitating pain is equally important for improving the quality of life of patients with spinal cord injury(SCI). Curren... In addition to restoration of bladder, bowel, and motor functions, alleviating the accompanying debilitating pain is equally important for improving the quality of life of patients with spinal cord injury(SCI). Currently,however, the treatment of chronic pain after SCI remains a largely unmet need. Electrical spinal cord stimulation(SCS) has been used to manage a variety of chronic pain conditions that are refractory to pharmacotherapy. Yet, its efficacy, benefit profiles, and mechanisms of action in SCI pain remain elusive, due to limited research, methodological weaknesses in previous clinical studies, and a lack of mechanistic exploration of SCS for SCI pain control. We aim to review recent studies and outline the therapeutic potential of different SCS paradigms for traumatic SCI pain. We begin with an overview of its manifestations,classification, potential underlying etiology, and currentchallenges for its treatment. The clinical evidence for using SCS in SCI pain is then reviewed. Finally, future perspectives of pre-clinical research and clinical study of SCS for SCI pain treatment are discussed. 展开更多
关键词 Pain TRAUMA SPINAL CORD injury SPINAL CORD stimulation NEUROMODULATION ANALGESIA
数据网络重构与云化演进的探索和实践 预览
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作者 屠礼彪 李彤 郭爱鹏 《邮电设计技术》 2019年第1期37-41,共5页
阐述了目前数据网络存在的问题,分析了传统数据业务面临的诸多挑战。提出了基于CORD的新型IP城域架构,并分析了基于新型架构的关键技术和主要特征,最后对中国联通数据网络重构和云化转型案例进行了分析。验证了在CORD的架构下,将SDN、NF... 阐述了目前数据网络存在的问题,分析了传统数据业务面临的诸多挑战。提出了基于CORD的新型IP城域架构,并分析了基于新型架构的关键技术和主要特征,最后对中国联通数据网络重构和云化转型案例进行了分析。验证了在CORD的架构下,将SDN、NFV技术引入城域网,采用DC为核心建设数据网络,推动云化转型才能解决传统网络的弊病。最后,对后续的演进提出了发展思路和对策。 展开更多
关键词 网络重构 CORD 网络云化 SDN NFV
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Micromechanical adaptation as a treatment for spinal cord injury 预览
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作者 Veronica Estrada Hans Werner Muller 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1909-1911,共3页
Spinal cord injury: Thus far injury of the spinal cord is incurable and, in the majority of cases, a devastating and life-changing event. The worldwide incidence rate of spinal cord injury (SCI) ranges from 250,000 to... Spinal cord injury: Thus far injury of the spinal cord is incurable and, in the majority of cases, a devastating and life-changing event. The worldwide incidence rate of spinal cord injury (SCI) ranges from 250,000 to 900,000 (www.who.int, 2013;Kumar et al., 2018) new cases per year. SCI outcome includes the damage of axons, demyelination of axons, loss of signal transduction, and consequential long-lasting motor and sensory deficits. Additionally, the non-use of muscles can lead to atrophy and joint contractures, thereby further reducing the possibility of recovery. Depending on the spinal level and the severity of the injury, the extent of the damage can vary and spontaneous recovery is possible to varying degrees. 展开更多
关键词 MICROMECHANICAL ADAPTATION SPINAL CORD INJURY SPINAL CORD injury(SCI)
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Use of longitudinal magnetic resonance imaging in preclinical models of spinal cord injury 预览
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作者 Harun Najib Noristani Florence Evelyne Perrin 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期771-772,共2页
Spinal cord injury(SCI)is a distressing event with grave socio/economic consequences to our society.Pathophysiological response following SCI involves blood-spinal cord barrier breakdown,neuroinflammation and formatio... Spinal cord injury(SCI)is a distressing event with grave socio/economic consequences to our society.Pathophysiological response following SCI involves blood-spinal cord barrier breakdown,neuroinflammation and formation of a glial scar that altogether govern the feasibility of spontaneous axonal re-growth and limited functional recovery.Great advances in understanding SCI pathophysiology have been achieved using numerous transgenic mouse lines developed in different strains.However,there are inherent strain differences that affect inflammation,gliosis,axon regeneration and ultimately functional recovery after SCI. 展开更多
关键词 SPINAL CORD injury(SCI) SPINAL CORD INJURY
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CNB-001 reduces paraplegia in rabbits following spinal cord ischemia 预览
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作者 Paul A. Lapchak Paul D. Boitano +5 位作者 Rene Bombien Daisy Chou Margot Knight Anja Muehle Mihaela Te Winkel Ali Khoynezhad 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2192-2198,共7页
aortic aneurysm repair and thoracic endovascular aortic repair results in devastating clinical deficits in patients. Because spinal cord ischemia is inadequately treated, we studied the effects of [4-((1E)-2-(5-(4-hyd... aortic aneurysm repair and thoracic endovascular aortic repair results in devastating clinical deficits in patients. Because spinal cord ischemia is inadequately treated, we studied the effects of [4-((1E)-2-(5-(4-hydroxy-3-methoxystyryl-)-1-phenyl-1H-pyrazoyl-3-yl) vinyl)-2-methoxy-phenol)](CNB-001), a novel curcumin-based compound, in a rabbit SCI model. CNB-001 is known to inhibit human 5-lipoxygenase and 15-lipoxygenase and reduce the ischemia-induced inflammatory response. Moreover, CNB- 001 can reduce the level of oxidative stress markers and potentiate brain-derived neurotrophic factor and brain-derived neurotrophic factor receptor signaling. The Tarlov scale and quantal analysis technique results revealed that CNB-001 administered as an intravenous dose (bolus) 30 minutes prior to spinal cord ischemia improved the behaviors of female New Zealand White rabbits. The improvements were similar to those produced by the uncompetitive N-methyl-D-aspartate receptor antagonist memantine. At 48 hours after aortic occlusion, there was a 42.7% increase (P < 0.05) in tolerated ischemia duration (n = 14) for rabbits treated with CNB-001 (n = 16), and a 72.3% increase for rabbits treated with the positive control memantine (P < 0.05)(n = 23) compared to vehicle-treated ischemic rabbits (n = 22). CNB-001 is a potential important novel treatment for spinal cord ischemia induced by aortic occlusion. All experiments were approved by the CSMC Institutional Animal Care and Use Committee (IACUC #4311) on November 1, 2012. 展开更多
关键词 curcumin analog SPINAL CORD injury SPINAL CORD ischemia thoraco-abdominal AORTIC aneurysm thoracic ENDOVASCULAR AORTIC repair motor function neuroprotection NEUROREPAIR
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The role of connexin43 in neuropathic pain induced by spinal cord injury
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作者 Anhui Wang Changshui Xu 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第6期555-561,共7页
Neuropathic pain is caused by the damage or dysfunction of the nervous system. In many neuropathic pain models, there is an increase in the number of gap junction (GJ) channels, especially the upregulation of the expr... Neuropathic pain is caused by the damage or dysfunction of the nervous system. In many neuropathic pain models, there is an increase in the number of gap junction (GJ) channels, especially the upregulation of the expression of connexin43 (Cx43), leading to the secretion of various types of cytokines and involvement in the formation of neuropathic pain. GJs are widely distributed in mammalian organs and tissues, and Cx43 is the most abundant connexin (Cx) in mammals. Astrocytes are the most abundant glial cell type in the central nervous system (CNS),which mainly express Cx43. More importantly, GJs play an important role in regulating cell metabolism, signaling, and function. Many existing literatures showed that Cx43 plays an important role in the nervous system, especially in the CNS under normal and pathological conditions. However, many internal mechanisms have not yet been thoroughly explored. In this review, we summarized the current understanding of the role and association of Cx and pannexin chan nets in neuropathic pain, especially after spinal cord injury, as well as some of our own in sights and thoughts which suggest that Cx43 may become an emerging therapeutic target for future neuropathic pain, bringing new hope to patients. 展开更多
关键词 connexin43 astrocytes gap junction NEUROPATHIC pain SPINAL CORD injury
Pain inhibition through transplantation of fetal neuronal progenitors into the injured spinal cord in rats 预览
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作者 Chary M. Batista Eric D. Mariano +6 位作者 Camila S. Dale Alexandre F. Cristante Luiz R. Britto Jose P. Otoch Manoel J. Teixeira Matthias Morgalla Guilherme Lepski 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期2011-2019,共9页
Neuropathic pain after spinal cord injury(SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy;nevertheless, the ideal cell type in terms of neuroge... Neuropathic pain after spinal cord injury(SCI) is a complex condition that responds poorly to usual treatments. Cell transplantation represents a promising therapy;nevertheless, the ideal cell type in terms of neurogenic potential and effectiveness against pain remains largely controversial. Here, we evaluated the ability of fetal neural stem cells(fNSC) to relieve chronic pain and, secondarily, their effects on motor recovery. Adult Wistar rats with traumatic SCI were treated, 10 days after injury, with intra-spinal injections of culture medium(sham) or fNSCs extracted from telencephalic vesicles(TV group) or the ventral medulla(VM group) of E/14 embryos. Sensory(von Frey filaments and hot plate) and motor(the Basso, Beattie,Bresnahan locomotor rating scale and inclined plane test) assessments were performed during 8 weeks. Thereafter, spinal cords were processed for immunofluorescence and transplanted cells were quantified by stereology. The results showed improvement of thermal hyperalgesia in the TV and VM groups at 4 and 5 weeks after transplantation, respectively. Moreover, mechanical allodynia improved in both the TV and VM groups at 8 weeks. No significant motor recovery was observed in the TV or VM groups compared with sham. Stereological analyses showed that ~70% of TV and VM cells differentiated into NeuN+ neurons,with a high proportion of enkephalinergic and GABAergic cells in the TV group and enkephalinergic and serotoninergic cells in the VM group. Our study suggests that neuronal precursors from TV and VM, once implanted into the injured spinal cord, maturate into different neuronal subtypes, mainly GABAergic, serotoninergic, and enkephalinergic, and all subtypes alleviate pain, despite no significant motor recovery. The study was approved by the Animal Ethics Committee of the Medical School of the University of S?o Paulo(protocol number 033/14) on March 4, 2016. 展开更多
关键词 spinal cord injuries chronic PAIN neural stem cells cell TRANSPLANTATION neuronal differentiation GABAERGIC NEURON serotoninergic NEURON enkephalinergic NEURON
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Changes in neurological and pathological outcomes in a modified rat spinal cord injury model with closed canal 预览
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作者 Xin Sun Xin Sun +5 位作者 Xing-Zhen Liu Jia Wang Hai-Rong Tao Tong Zhu Wen-Jie Jin Kang-Ping Shen 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期697-704,共8页
Most animal spinal cord injury models involve a laminectomy,such as the weight drop model or the transection model.However,in clinical practice,many patients undergo spinal cord injury while maintaining a relatively c... Most animal spinal cord injury models involve a laminectomy,such as the weight drop model or the transection model.However,in clinical practice,many patients undergo spinal cord injury while maintaining a relatively complete spinal canal.Thus,open spinal cord injury models often do not simulate real injuries,and few previous studies have investigated whether having a closed spinal canal after a primary spinal cord injury may influence secondary processes.Therefore,we aimed to assess the differences in neurological dysfunction and pathological changes between rat spinal cord injury models with closed and open spinal canals.Sprague-Dawley rats were randomly divided into three groups.In the sham group,the tunnel was expanded only,without inserting a screw into the spinal canal.In the spinal cord injury with open canal group,a screw was inserted into the spinal canal to cause spinal cord injury for 5 minutes,and then the screw was pulled out,leaving a hole in the vertebral plate.In the spinal cord injury with closed canal group,after inserting a screw into the spinal canal for 5 minutes,the screw was pulled out by approximately 1.5 mm and the flat end of the screw remained in the hole in the vertebral plate so that the spinal canal remained closed;this group was the modified model,which used a screw both to compress the spinal cord and to seal the spinal canal.At 7 days post-operation,the Basso-Beattie-Bresnahan scale was used to measure changes in neurological outcomes.Hematoxylin-eosin staining was used to assess histopathology.To evaluate the degree of local secondary hypoxia,immunohistochemical staining and western blot assays were applied to detect the expression of hypoxia-inducible factor 1α(HIF-1α)and vascular endothelial growth factor(VEGF).Compared with the spinal cord injury with open canal group,in the closed canal group the Basso-Beattie-Bresnahan scores were lower,cell morphology was more irregular,the percentage of morphologically normal neurons was lower,the percentages of HIF-1α-and VEGF-immunorea 展开更多
关键词 Basso-Beattie-Bresnahan scores closed spinal canal HIF-1α hypoxia MODEL nerve regeneration open spinal canal rat secondary injury spinal cord injury VEGF
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Spatiotemporal expression of leukemia inhibitory factor receptor protein during neural tube development in embryos with neural tube defects 预览
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作者 Dong An Xiao-Wei Wei +3 位作者 He-Nan Zhang Dan Liu Wei Ma Zheng-Wei Yuan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期705-711,共7页
Leukemia inhibitory factor receptor(LIFR),as a neuroregulatory cytokine receptor,generally shows a neuroprotective effect in central nervous system injuries.In this study,to understand the effect of LIFR on pathogenes... Leukemia inhibitory factor receptor(LIFR),as a neuroregulatory cytokine receptor,generally shows a neuroprotective effect in central nervous system injuries.In this study,to understand the effect of LIFR on pathogenesis of neural tube defects,we explored spatiotemporal expression of LIFR at different stages of fetal development in normal and neural tube defect embryos.Spina bifida aperta was induced with all-trans retinoic acid on embryonic day 10 in rats,and the spatiotemporal expression of LIFR was investigated in spina bifida aperta rats and healthy rats from embryonic day 11 to 17.Real time-polymerase chain reaction and western blot assay were used to examine mRNA and protein expression of LIFR in healthy control and neural tube defect embryos.Results of the animal experiment demonstrated that expression of LIFR protein and mRNA in the spinal cords of normal rat embryos increased with embryonic development.LIFR was significantly downregulated in the spinal cords of spina bifida aperta rats compared with healthy rats from embryonic days 11 to 17.Immunohistochemical staining showed that the expression of LIFR in placenta and spinal cord in spina bifida aperta rat embryos was decreased compared with that in control embryos at embryonic day 15.Results from human embryo specimens showed that LIFR mRNA expression was significantly down-regulated in spinal cords of human fetuses with neural tube defects compared with normal controls at a gestational age of 24 to 33 weeks.The results were consistent with the down-regulation of LIFR in the animal experiments.Our study revealed spatiotemporal changes in expression of LIFR during embryonic neurulation.Thus,LIFR might play a specific role in neural tube development.All animal and human experimental procedures were approved by the Medical Ethics Committee of Shengjing Hospital of China Medical University,China(approval No.2016PS106K)on February 25,2016. 展开更多
关键词 amniotic fluid DEVELOPMENT EMBRYOGENESIS leukemia inhibitory factor receptor nerve regeneration neural tube defect PLACENTA spatiotemporal expression spina bifida aperta spinal cord serum
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Effects of miR-219/miR-338 on microglia and astrocyte behaviors and astrocyte-oligodendrocyte precursor cell interactions 预览
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作者 Lan Huong Nguyen William Ong +3 位作者 Kai Wang Mingfeng Wang Dean Nizetic Sing Yian Chew 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期739-747,共9页
MiR-219 and miR-338(miR-219/miR-338)are oligodendrocyte-specific microRNAs.The overexpression of these miRs in oligodendrocyte precursor cells promotes their differentiation and maturation into oligodendrocytes,which ... MiR-219 and miR-338(miR-219/miR-338)are oligodendrocyte-specific microRNAs.The overexpression of these miRs in oligodendrocyte precursor cells promotes their differentiation and maturation into oligodendrocytes,which may enhance axonal remyelination after nerve injuries in the central nervous system(CNS).As such,the delivery of miR-219/miR-338 to the CNS to promote oligodendrocyte precursor cell differentiation,maturation and myelination could be a promising approach for nerve repair.However,nerve injuries in the CNS also involve other cell types,such as microglia and astrocytes.Herein,we investigated the effects of miR-219/miR-338 treatment on microglia and astrocytes in vitro and in vivo.We found that miR-219/miR-338 diminished microglial expression of pro-inflammatory cytokines and suppressed astrocyte activation.In addition,we showed that miR-219/miR-338 enhanced oligodendrocyte precursor cell differentiation and maturation in a scratch assay paradigm that re-created a nerve injury condition in vitro.Collectively,our results suggest miR-219/miR-338 as a promising treatment for axonal remyelination in the CNS following nerve injuries.All experimental procedures were approved by the Institutional Animal Care and Use Committee(IACUC),Nanyang Technological University(approval No.A0309 and A0333)on April 27,2016 and October 8,2016. 展开更多
关键词 central nervous system electrospinning gene silencing GLIA hydrogel MYELINATION nanofibers oligodendroglial POLYCAPROLACTONE spinal cord injury
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Effects of neural stem cell transplantation on the motor function of rats with contusion spinal cord injuries:a meta-analysis 预览
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作者 Kai Qian Tuo-Ye Xu +7 位作者 Xi Wang Tao Ma Kai-Xin Zhang Kun Yang Teng-Da Qian Jing Shi Li-Xin Li Zheng Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期748-758,共11页
Objective:To judge the efficacies of neural stem cell(NSC)transplantation on functional recovery following contusion spinal cord injuries(SCIs).Data sources:Studies in which NSCs were transplanted into a clinically re... Objective:To judge the efficacies of neural stem cell(NSC)transplantation on functional recovery following contusion spinal cord injuries(SCIs).Data sources:Studies in which NSCs were transplanted into a clinically relevant,standardized rat model of contusion SCI were identified by searching the PubMed,Embase and Cochrane databases,and the extracted data were analyzed by Stata 14.0.Data selection:Inclusion criteria were that NSCs were used in in vivo animal studies to treat contusion SCIs and that behavioral assessment of locomotor functional recovery was performed using the Basso,Beattie,and Bresnahan lo-comotor rating scale.Exclusion criteria included a follow-up of less than 4 weeks and the lack of control groups.Outcome measures:The restoration of motor function was assessed by the Basso,Beattie,and Bresnahan locomotor rating scale.Results:We identified 1756 non-duplicated papers by searching the aforementioned electronic databases,and 30 full-text articles met the inclusion criteria.A total of 37 studies reported in the 30 articles were included in the meta-analysis.The meta-analysis results showed that transplanted NSCs could improve the motor function recovery of rats following contusion SCIs,to a moderate extent(pooled standardized mean difference(SMD)=0.73;95%confidence interval(CI):0.47–1.00;P<0.001).NSCs obtained from different donor species(rat:SMD=0.74;95%CI:0.36–1.13;human:SMD=0.78;95%CI:0.31–1.25),at different donor ages(fetal:SMD=0.67;95%CI:0.43–0.92;adult:SMD=0.86;95%CI:0.50–1.22)and from different origins(brain-derived:SMD=0.59;95%CI:0.27–0.91;spinal cord-derived:SMD=0.51;95%CI:0.22–0.79)had similar efficacies on improved functional recovery;however,adult induced pluripotent stem cell-derived NSCs showed no significant efficacies.Furthermore,the use of higher doses of transplanted NSCs or the administration of immunosuppressive agents did not promote better locomotor function recovery(SMD=0.45;95%CI:0.21–0.70).However,shorter periods between the contusion induction and the NSC tr 展开更多
关键词 Basso Beattie and Bresnahan locomotor rating scale cell transplantation META-ANALYSIS motor functional recovery neural regeneration neural stem cell neural stem cell transplantation rat model spinal contusion spinal cord injury
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Unfolded protein response in myelin disorders 预览
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作者 Wensheng Lin Sarrabeth Stone 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期636-645,共10页
Activation of the unfolded protein response in response to endoplasmic reticulum stress preserves cell viability and function under stressful conditions.Nevertheless,persistent,unresolvable activation of the unfolded ... Activation of the unfolded protein response in response to endoplasmic reticulum stress preserves cell viability and function under stressful conditions.Nevertheless,persistent,unresolvable activation of the unfolded protein response can trigger apoptosis to eliminate stressed cells.Recent studies show that the unfolded protein response plays an important role in the pathogenesis of various disorders of myelin,including multiples sclerosis,Charcot-Marie-Tooth disease,Pelizaeus-Merzbacher disease,vanishing white matter disease,spinal cord injury,tuberous sclerosis complex,and hypoxia-induced perinatal white matter injury.In this review we summarize the current literature on the unfolded protein response and the evidence for its role in the pathogenesis of myelin disorders. 展开更多
关键词 AXON ER multiples sclerosis MYELIN OLIGODENDROCYTE Schwann cell spinal cord injury UPR
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氟磷酸盐光学玻璃漏料管设计及流量控制的研究 预览
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作者 姜敬陆 李攀 +2 位作者 徐华峰 徐光以 王拓 《玻璃与搪瓷》 CAS 2019年第2期1-5,10共6页
选取氟磷酸盐光学玻璃的漏料成型作为研究对象,针对氟磷酸盐光学玻璃的漏料成型过程中,玻璃易产生析晶和成型条纹而进行设计和研究。通过对漏料管长度及管径的计算和设计,达到控制玻璃流量、提高产品质量的目的。
关键词 氟磷酸盐玻璃 漏料管 温黏曲线 析晶 条纹
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Sciatic nerve injury alters the spatial arrangement of neurons and glial cells in the anterior horn of the spinal cord 预览
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作者 Ali Rashidiani-Rashidabadi Mohammad Hassan Heidari +7 位作者 Ensieh Sajadi Fatemeh Hejazi Fatemeh Fadaei Fathabady Yousef Sadeghi Abbas Aliaghaei Amir Raoofi Mohammad-Amin Abdollahifar Reza Mastery Farahni 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1833-1840,共8页
The spatial arrangement of the cell is important and considered as underlying mechanism for mathematical modeling of cell to cell interaction.The ability of cells to take on the characteristics of other cells in an or... The spatial arrangement of the cell is important and considered as underlying mechanism for mathematical modeling of cell to cell interaction.The ability of cells to take on the characteristics of other cells in an organism,it is important to understand the dynamical behavior of the cells.This method implements experimental parameters of the cell-cell interaction into the mathematical simulation of cell arrangement.The purpose of this research was to explore the three-dimensional spatial distribution of anterior horn cells in the rat spinal cord to examine differences after sciatic nerve injury.Sixteen Sprague-Dawley male rats were assigned to control and axotomy groups.Twelve weeks after surgery,the anterior horn was removed for first-and second-order stereological studies.Second-order stereological techniques were applied to estimate the pair correlation and cross-correlation functions using a dipole probe superimposed onto the spinal cord sections.The findings revealed 7% and 36% reductions in the mean volume and total number of motoneurons,respectively,and a25% increase in the neuroglial cell number in the axotomized rats compared to the control rats.In contrast,the anterior horn volume remained unchanged.The results also indicated a broader gap in the pair correlation curve for the motoneurons and neuroglial cells in the axotomized rats compared to the control rats.This finding shows a negative correlation for the distribution of motoneurons and neuroglial cells in the axotomized rats.The cross-correlation curve shows a negative correlation between the motoneurons and neuroglial cells in the axotomized rats.These findings suggest that cellular structural and functional changes after sciatic nerve injury lead to the alterations in the spatial arrangement of motoneurons and neuroglial cells,finally affecting the normal function of the central nervous system.The experimental protocol was reviewed and approved by the Animal Ethics Committee of Shahid Beheshti University of Medical Sciences(approval No.IR.SBMU.MS 展开更多
关键词 SCIATIC nerve injury spatial arrangement spinal cord MOTORNEURON neuroglial cells AXOTOMY ANTERIOR HORN
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RNA sequencing screening of differentially expressed genes after spinal cord injury 预览
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作者 Yi Li Ying Chen +5 位作者 Xiang Li Jian Wu Jing-Ying Pan Ri-Xin Cai Ri-Yun Yang Xiao-Dong Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1583-1593,共11页
In the search for a therapeutic schedule for spinal cord injury,it is necessary to understand key genes and their corresponding regulatory networks involved in the spinal cord injury process.However,ad hoc selection a... In the search for a therapeutic schedule for spinal cord injury,it is necessary to understand key genes and their corresponding regulatory networks involved in the spinal cord injury process.However,ad hoc selection and analysis of one or two genes cannot fully reveal the complex molecular biological mechanisms of spinal cord injury.The emergence of second-generation sequencing technology(RNA sequencing)has provided a better method.In this study,RNA sequencing technology was used to analyze differentially expressed genes at different time points after spinal cord injury in rat models established by contusion of the eighth thoracic segment.The numbers of genes that changed significantly were 944,1362 and 1421 at 1,4 and 7 days after spinal cord injury respectively.After gene ontology analysis and temporal expression analysis of the differentially expressed genes,C5ar1,Socs3 and CCL6 genes were then selected and identified by real-time polymerase chain reaction and western blot assay.The mRNA expression trends of C5ar1,Socs3 and CCL6 genes were consistent with the RNA sequencing results.Further verification and analysis of C5ar1 indicate that the level of protein expression of C5ar1 was consistent with its nucleic acid level after spinal cord injury.C5ar1 was mainly expressed in neurons and astrocytes.Finally,the gene Itgb2,which may be related to C5ar1,was found by Chilibot database and literature search.Immunofluorescence histochemical results showed that the expression of Itgb2 was highly consistent with that of C5ar1.Itgb2 was expressed in astrocytes.RNA sequencing technology can screen differentially expressed genes at different time points after spinal cord injury.Through analysis and verification,genes strongly associated with spinal cord injury can be screened.This can provide experimental data for further determining the molecular mechanism of spinal cord injury,and also provide possible targets for the treatment of spinal cord injury.This study was approved ethically by the Laboratory Animal Ethics Commit 展开更多
关键词 nerve REGENERATION SPINAL CORD injury RNA sequencing C5ar1 Itgb2 SOCS3 CCL6 ASTROCYTES rats neural REGENERATION
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Identification of microRNAs and messenger RNAs involved in human umbilical cord mesenchymal stem cell treatment of ischemic cerebral infarction using integrated bioinformatics analysis 预览
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作者 Yin-Meng Qu Xin Sun +3 位作者 Xiu-Li Yan Hang Jin Zhen-Ni Guo Yi Yang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1610-1616,共7页
In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are i... In recent years,a large number of differentially expressed genes have been identified in human umbilical cord mesenchymal stem cell(hUMSC)transplants for the treatment of ischemic cerebral infarction.These genes are involved in various biochemical processes,but the role of microRNAs(miRNAs)in this process is still unclear.From the Gene Expression Omnibus(GEO)database,we downloaded two microarray datasets for GSE78731(messenger RNA(mRNA)profile)and GSE97532(miRNA profile).The differentially expressed genes screened were compared between the hUMSC group and the middle cerebral artery occlusion group.Gene ontology enrichment and pathway enrichment analyses were subsequently conducted using the online Database for Annotation,Visualization,and Integrated Discovery.Identified genes were applied to perform weighted gene co-suppression analyses,to establish a weighted co-expression network model.Furthermore,the protein-protein interaction network for differentially expressed genes from turquoise modules was built using Cytoscape(version 3.40)and the most highly correlated subnetwork was extracted from the protein-protein interaction network using the MCODE plugin.The predicted target genes for differentially expressed miRNAs were also identified using the online database starBase v3.0.A total of 3698 differentially expressed genes were identified.Gene ontology analysis demonstrated that differentially expressed genes that are related to hUMSC treatment of ischemic cerebral infarction are involved in endocytosis and inflammatory responses.We identified 12 differentially expressed miRNAs in middle cerebral artery occlusion rats after hUMSC treatment,and these differentially expressed miRNAs were mainly involved in signaling in inflammatory pathways,such as in the regulation of neutrophil migration.In conclusion,we have identified a number of differentially expressed genes and differentially expressed mRNAs,miRNA-mRNAs,and signaling pathways involved in the hUMSC treatment of ischemic cerebral infarction.Bioinformatics and 展开更多
关键词 nerve REGENERATION ischemic cerebral infarction human umbilical cord mesenchymal STEM CELL TREATMENT bioinformatics analysis DIFFERENTIALLY EXPRESSED genes DIFFERENTIALLY EXPRESSED mRNAs inflammatory response STEM CELL therapy weighted gene co-suppression analysis WGCNA protein-protein interaction network PPI hUMSC neural REGENERATION
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Transplantation of adult spinal cord tissue: Transection spinal cord repair and potential clinical translation
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作者 Hang Lin 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第6期870-872,共3页
Spinal cord injury (SCI), often resulting from car accidents,falls and violence, represents a complicated trauma affecting multiple tissues, such as the vertebrate, disks of the spinal column, or the spinal cord itsel... Spinal cord injury (SCI), often resulting from car accidents,falls and violence, represents a complicated trauma affecting multiple tissues, such as the vertebrate, disks of the spinal column, or the spinal cord itself. Direct injury to the spinal cord, which is the focus of this insight, may disrupt the neuronal connections between the brain and the periphery,leading to the loss of motor function and paralysis. Consequently, SCI patients suffer neurologic deficits and disability,and are subjected to high healthcare costs for a lifetime. 展开更多
关键词 SPINAL CORD injury car accidents COSTS for a LIFETIME
Bioinformatics analyses of differentially expressed genes associated with spinal cord injury:a microarray-based analysis in a mouse model 预览
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作者 Lei Guo Jing Lv +2 位作者 Yun-Fei Huang Ding-Jun Hao Ji-Jun Liu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1262-1270,共9页
Gene spectrum analysis has shown that gene expression and signaling pathways change dramatically after spinal cord injury,which may affect the microenvironment of the damaged site.Microarray analysis provides a new op... Gene spectrum analysis has shown that gene expression and signaling pathways change dramatically after spinal cord injury,which may affect the microenvironment of the damaged site.Microarray analysis provides a new opportunity for investigating diagnosis,treatment,and prognosis of spinal cord injury.However,differentially expressed genes are not consistent among studies,and many key genes and signaling pathways have not yet been accurately studied.GSE5296 was retrieved from the Gene Expression Omnibus DataSet.Differentially expressed genes were obtained using R/Bioconductor software(expression changed at least two-fold;P<0.05).Database for Annotation,Visualization and Integrated Discovery was used for functional annotation of differentially expressed genes and Animal Transcription Factor Database for predicting potential transcription factors.The resulting transcription regulatory protein interaction network was mapped to screen representative genes and investigate their diagnostic and therapeutic value for disease.In total,this study identified 109 genes that were upregulated and 30 that were downregulated at 0.5,4,and 24 hours,and 3,7,and 28 days after spinal cord injury.The number of downregulated genes was smaller than the number of upregulated genes at each time point.Database for Annotation,Visualization and Integrated Discovery analysis found that many inflammation-related pathways were upregulated in injured spinal cord.Additionally,expression levels of these inflammation-related genes were maintained for at least 28 days.Moreover,399 regulation modes and 77 nodes were shown in the protein-protein interaction network of upregulated differentially expressed genes.Among the 10 upregulated differentially expressed genes with the highest degrees of distribution,six genes were transcription factors.Among these transcription factors,ATF3 showed the greatest change.ATF3 was upregulated within 30 minutes,and its expression levels remained high at 28 days after spinal cord injury.These key genes screened by bioinf 展开更多
关键词 nerve REGENERATION spinal cord injury differentially expressed GENES BIOINFORMATICS ANALYSES Database for Annotation Visualization and Integrated Discovery ANALYSIS inflammation Kyoto Encyclopedia of GENES and Genomes pathway MICROARRAY transcription factors neural REGENERATION
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