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Endoscopic resection techniques for colorectal neoplasia:Current developments 预览
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作者 Franz Ludwig Dumoulin Ralf Hildenbrand 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第3期300-307,共8页
Endoscopic polypectomy and endoscopic mucosal resection(EMR)are the established treatment standards for colorectal polyps.Current research aims at the reduction of both complication and recurrence rates as well as on ... Endoscopic polypectomy and endoscopic mucosal resection(EMR)are the established treatment standards for colorectal polyps.Current research aims at the reduction of both complication and recurrence rates as well as on shortening procedure times.Cold snare resection is the emerging standard for the treatment of smaller(<5mm)polyps and is possibly also suitable for the removal of noncancerous polyps up to 9 mm.The method avoids thermal damage,has reduced procedure times and probably also a lower risk for delayed bleeding.On the other end of the treatment spectrum,endoscopic submucosal dissection(ESD)offers en bloc resection of larger flat or sessile lesions.The technique has obvious advantages in the treatment of high-grade dysplasia and early cancer.Due to its minimal recurrence rate,it may also be an alternative to fractionated EMR of larger flat or sessile lesions.However,ESD is technically demanding and burdened by longer procedure times and higher costs.It should therefore be restricted to lesions suspicious for high-grade dysplasia or early invasive cancer.The latest addition to endoscopic resection techniques is endoscopic fullthickness resection with specifically developed devices for flexible endoscopy.This method is very useful for the treatment of smaller difficult-to-resect lesions,e.g.,recurrence with scar formation after previous endoscopic resections. 展开更多
关键词 COLORECTAL NEOPLASIA COLORECTAL cancer screening Cold SNARE RESECTION ENDOSCOPIC POLYPECTOMY ENDOSCOPIC mucosal RESECTION ENDOSCOPIC submucosal dissection ENDOSCOPIC full-thickness RESECTION Adenoma recurrence rate
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Preoperative bowel preparation does not favor the management of colorectal anastomotic leak 预览
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作者 Konstantinos A Zorbas Daohai Yu +2 位作者 Aruj Choudhry Howard M Ross Matthew Philp 《世界胃肠外科杂志:英文版(电子版)》 2019年第4期218-228,共11页
BACKGROUND Controversy exists regarding the impact of preoperative bowel preparation on patients undergoing colorectal surgery. This is due to previous research studies, which fail to demonstrate protective effects of... BACKGROUND Controversy exists regarding the impact of preoperative bowel preparation on patients undergoing colorectal surgery. This is due to previous research studies, which fail to demonstrate protective effects of mechanical bowel preparation against postoperative complications. However, in recent studies, combination therapy with oral antibiotics (OAB) and mechanical bowel preparation seems to be beneficial for patients undergoing an elective colorectal operation. AIM To determine the association between preoperative bowel preparation and postoperative anastomotic leak management (surgical vs non-surgical). METHODS Patients with anastomotic leak after colorectal surgery were identified from the 2013 and 2014 Colectomy Targeted American College of Surgeons National Surgical Quality Improvement Program (ACS-NSQIP) database and were employed for analysis. Every patient was assigned to one of three following groups based on the type of preoperative bowel preparation: first groupmechanical bowel preparation in combination with OAB, second groupmechanical bowel preparation alone, and third group-no preparation. RESULTS A total of 652 patients had anastomotic leak after a colectomy from January 1, 2013 through December 31, 2014. Baseline characteristics were assessed andfound that there were no statistically significant differences between the three groups in terms of age, gender, race, American Society of Anesthesiologists score, and other preoperative characteristics. A χ2 test of homogeneity was conducted and there was no statistically/clinically significant difference between the three categories of bowel preparation in terms of reoperation. CONCLUSION The implementation of mechanical bowel preparation and antibiotic use in patients who are going to undergo a colon resection does not influence the treatment of any possible anastomotic leakage. 展开更多
关键词 PREOPERATIVE BOWEL PREPARATION PREOPERATIVE mechanical BOWEL PREPARATION PREOPERATIVE oral antibiotics COLORECTAL anastomotic LEAK Anastomotic LEAK BOWEL PREPARATION COLORECTAL surgery National Surgical Quality Improvement Program
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Patrinia scabiosaefolia Inhibits Growth of 5-FU-Resistant Colorectal Carcinoma Cells via Induction of Apoptosis and Suppression of AKT Pathway
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作者 HUANG Si-zhou LIU Wang-yu +3 位作者 HUANG Yue SHEN A-ling LIU Li-ya PENG Jun 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第2期116-121,共6页
Objective: To investigate the effects of ethanol extract of Patrinia scabiosaefolia(EEPS) on chemo-resistance of colorectal cancer cells(CRC) and explore the possible molecular mechanisms. Methods: 5-fluorouracil(5-FU... Objective: To investigate the effects of ethanol extract of Patrinia scabiosaefolia(EEPS) on chemo-resistance of colorectal cancer cells(CRC) and explore the possible molecular mechanisms. Methods: 5-fluorouracil(5-FU)-resistant human colorectal carcinoma cell line(HCT-8/5-FU) and its parental cells HCT-8 were treated with EEPS(0, 0.25, 0.50, 1 or 2 mg/mL), or 5-FU(0, 100, 200, 400, 800 or 1600 μmol/L). The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to evaluate the cell viability. Cell density was observed by phase-contrast microscope, cell counting and colony formation assay were used to determine the cell proliferation of HCT-8/5-FU cells treated with 0, 0.5, 1 or 2 mg/mL EEPS. Cell apoptosis was determined by Hoechst staining. Western-blot was performed to detect the phosphorylation of AKT as well as the protein expression level of B-cell CLL/lymphoma 2(Bcl-2) and Bcl-2-associated X protein(Bax). Results: Compared with HCT-8 cells, MTT assay results indicated that HCT-8/5-FU cells were resistant to 5-FU treatment(P<0.05), and sensitive to EEPS treatment(P>0.05). Moreover, compared with untreated HCT-8/5-FU cells, 1 and 2 mg/mL of EEPS treatment significantly reduced cell density, cell number, inhibited cell survival(P<0.05), and induced apoptosis in HCT-8/5-FU cells. Furthermore, 1 and 2 mg/mL of EEPS significantly decreased the phosphorylation level of p-AKT and Bcl-2 protein expression, and increased the expression of Bax protein(P<0.05). Conclusion: EEPS is a promising therapeutic agent that may overcome chemo-resistance in cancer cells, likely through suppression of the AKT pathway and promotion of cancer cell apoptosis. 展开更多
关键词 AKT PATHWAY COLORECTAL cancer 5-FLUOROURACIL resistance Patrinia scabiosaefolia Chinese medicine
Stent placement followed by preoperative chemotherapy and elective surgery for acute malignant colorectal obstruction: Six cases of report 预览
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作者 Jun-Jie Liu Teng-Hui Ma +1 位作者 Qi-Yuan Qin Lei Wang 《世界胃肠肿瘤学杂志:英文版(电子版)》 CAS 2019年第3期264-269,共6页
BACKGROUND The self-expandable metal stent is used as a bridge to surgery in the treatment of acute malignant colorectal obstruction(AMCO). However, recent studies have shown inferior long-term outcomes and increased ... BACKGROUND The self-expandable metal stent is used as a bridge to surgery in the treatment of acute malignant colorectal obstruction(AMCO). However, recent studies have shown inferior long-term outcomes and increased risk of tumor dissemination after stent placement. In addition, the optimal interval between stent placement and surgery is not clear. The aim of the current study was to present a new strategy for AMCO: stent placement followed by preoperative chemotherapy and elective surgery.CASE SUMMARY Six patients were diagnosed as acute obstruction. There was one patient with descending cancer, four with sigmoid cancers and one with rectal cancer. The obstructive symptoms of these six patients were relieved within 3 d after stent placement. After receiving two cycles of preoperative chemotherapy, consisting of modified infusional fluorouracil, leucovorin and oxaliplatin [modified FOLFOX6(mFOLFOX6)], they underwent elective surgery of primary tumor resection. None of the 6 patients received colostomy or colonic lavage during surgery. There was no complication of anastomotic leak, ileus or surgical site infection after surgery. In addition, the patients had low operation time and blood loss, adequate lymph nodes harvest and fast postoperative recovery.CONCLUSION The two-cycle mFOLFOX6 preoperative chemotherapy and elective surgery after stent placement is a safe and feasible strategy in the management of AMCO. 展开更多
关键词 STENT COLORECTAL cancer OBSTRUCTION PREOPERATIVE chemotherapy Case REPORT
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Tertiary stent-in-stent for obstructing colorectal cancer: A case report and literature review 预览
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作者 Giuseppe Vanella Chiara Coluccio +2 位作者 Emilio Di Giulio Daniela Assisi Rocco Lapenta 《世界胃肠内镜杂志:英文版(电子版)》 2019年第1期61-67,共7页
BACKGROUND Self-expandable metal stents(SEMSs) are frequently used in the setting of palliation for occluding, inoperable colorectal cancer(CRC). Among possible complications of SEMS positioning, re-obstruction is the... BACKGROUND Self-expandable metal stents(SEMSs) are frequently used in the setting of palliation for occluding, inoperable colorectal cancer(CRC). Among possible complications of SEMS positioning, re-obstruction is the most frequent. Its management is controversial, potentially involving secondary stent-in-stent placement, which has been poorly investigated. Moreover, the issue of secondary stent-in-stent re-obstruction and of more-than-two colonic stenting has never been assessed. We describe a case of tertiary SEMS-in-SEMS placement, and also discuss our practice based on available literature.CASE SUMMARY A 66-year-old male with occluding and metastatic CRC was initially treated by positioning of a SEMS, which had to be revised 6 mo later when a symptomatic intra-stent tumor ingrowth was treated by a SEMS-in-SEMS. We hereby describe an additional episode of intestinal occlusion due to recurrence of intra-stent tumor ingrowth. This patient, despite several negative prognostic factors(splenic flexure location of the tumor, carcinomatosis with ascites, subsequent chemotherapy that included bevacizumab and two previously positioned stents(1 SEMS and 1 SEMS-in-SEMS)) underwent successful management through the placement of a tertiary SEMS-in-SEMS, with immediate clinical benefit and no procedure-related adverse events after 150 d of post-procedural follow-up. This endoscopic management has permitted 27 mo of partial control of a metastatic disease without the need for chemotherapy discontinuation and, ultimately, a good quality of life until death.CONCLUSION Tertiary SEMS-in-SEMS is technically feasible, and appears to be a safe and effective option in the case of recurrent SEMS obstruction. 展开更多
关键词 BEVACIZUMAB Colorectal neoplasms Intestinal OBSTRUCTION PALLIATIVE care Self-expandable metallic STENTS Case report
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Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice 预览
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作者 Chang Xu Kun Wang +2 位作者 Yu-Han Ding Wen-Jing Li Lei Ding 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第5期584-599,共16页
BACKGROUND Claudin-7,one of the important components of cellular tight junctions,is currently considered to be expressed abnormally in colorectal inflammation and colorectal cancer.However,there is currently no effect... BACKGROUND Claudin-7,one of the important components of cellular tight junctions,is currently considered to be expressed abnormally in colorectal inflammation and colorectal cancer.However,there is currently no effective animal model to study its specific mechanism.Therefore,we constructed three lines of Claudin-7 knockout mice using the Cre/LoxP system.AIM To determine the function of the tumor suppressor gene Claudin-7 by generating three lines of Claudin-7 gene knockout mice.METHODS We crossed Claudin-7-floxed mice with CMV-Cre,vil1-Cre,and villin-CreERT2 transgenic mice,and the offspring were self-crossed to obtain conventional Claudin-7 knockout mice,conditional(intestinal specific)Claudin-7 knockout mice,and inducible conditional Claudin-7 knockout mice.Intraperitoneal injection of tamoxifen into the inducible conditional Claudin-7 knockout mice can induce the knockout of Claudin-7.PCR and agarose gel electrophoresis were used to identify mouse genotypes,and Western blot was used to confirm the knockout of Claudin-7.The mental state,body length,and survival time of these mice were observed.The dying mice were sacrificed,and hematoxylin-eosin(HE)staining and immunohistochemical staining were performed to observe changes in intestinal structure and proliferation markers.RESULTS We generated Claudin-7-floxed mice and three lines of Claudin-7 gene knockout mice using the Cre/LoxP system successfully.Conventional and intestinal specific Claudin-7 knockout mice were stunted and died during the perinatal period,and intestinal HE staining in these mice revealed mucosal gland structure disappearance and connective tissue hyperplasia with extensive inflammatory cell infiltration.The inducible conditional Claudin-7 knockout mice had a normal phenotype at birth,but after the induction with tamoxifen,they exhibited a dying state.Intestinal HE staining showed significant inflammatory cell infiltration,and atypical hyperplasia and adenoma were also observed.Intestinal immunohistochemistry analysis showed abnormal expressi 展开更多
关键词 Claudin-7 Gene KNOCKOUT Inflammation ADENOMAS Colorectal carcinoma
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Relationships among KRAS mutation status,expression of RAS pathway signaling molecules,and clinicopathological features and prognosis of patients with colorectal cancer 预览
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作者 Xiang-Bin Wan Ai-Qin Wang +6 位作者 Jian Cao Zhi-Chuang Dong Ning Li Sen Yang Miao-Miao Sun Zhi Li Su-Xia Luo 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第7期808-823,共16页
BACKGROUND The RAS/RAF/MEK/ERK and PI3K/AKT/mTOR signaling pathways all belong to mitogen-activated protein kinase(MAPK)signaling pathways,Mutations in any one of the upstream genes(such as the RAS gene or the BRAF ge... BACKGROUND The RAS/RAF/MEK/ERK and PI3K/AKT/mTOR signaling pathways all belong to mitogen-activated protein kinase(MAPK)signaling pathways,Mutations in any one of the upstream genes(such as the RAS gene or the BRAF gene)may be transmitted to the protein through transcription or translation,resulting in abnormal activation of the signaling pathway.This study investigated the relationship between the KRAS gene mutation and the clinicopathological features and prognosis of colorectal cancer(CRC),and the effect of KRAS mutations on its associated proteins in CRC,with an aim to clarify the cause of tumor progression and drug resistance caused by mutation of the KRAS gene.AIM To investigate the KRAS gene and RAS pathway signaling molecules in CRC and to analyze their relationship with clinicopathological features and prognosis METHODS Colorectal cancer tissue specimens from 196 patients were analyzed for KRAS mutations using quantitative polymerase chain reaction and for KRAS,BRAF,MEK,and ERK protein expression levels using immunohistochemistry of tumor microarrays.To analyze differences of RAS pathway signaling molecule expression levels in different KRAS gene status,the relationships between these parameters and clinicopathological features,4-year progression-free survival,and overall survival were analyzed by independent sample t test,Kaplan-Meier plots,and the log-rank test.Predictors of overall and disease-free survival were assessed using a Cox proportional hazards model.RESULTS Of the 196 patients,62(32%)carried mutations in codon 12(53/62)or codon 13(9/62)in exon 2 of the KRAS gene.KRAS,BRAF,ERK,and MEK protein expression was detected in 71.4%,78.8%,64.3%,and 50.8%of CRC tissues,respectively.There were no significant differences between KRAS mutation status and KRAS,BRAF,MEK,or ERK protein levels.Positive expression of KRAS and ERK was associated with poor tumor differentiation,and KRAS expression was also associated with age<56 years.MEK expression was significantly associated with distant metastasis(P<0.05).T 展开更多
关键词 Colorectal cancer KRAS gene KRAS PROTEIN BRAF PROTEIN MEK PROTEIN ERK PROTEIN
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竖脊肌平面阻滞对腹腔镜结直肠癌根治术患者细胞免疫功能的影响 预览
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作者 叶克平 叶琦刚 +3 位作者 王文伟 李梅 王以瑞 郑芝 《浙江医学》 CAS 2019年第10期1033-1036,1044共5页
目的 探讨竖脊肌平面阻滞(ESP)对腹腔镜结直肠癌根治术患者细胞免疫功能的影响。方法 选择60例择期拟行腹腔镜结直肠癌根治术患者,采用随机数字表法分为3组:竖脊肌平面阻滞复合全凭静脉麻醉组(ESP组)、硬膜外阻滞复合全凭静脉麻醉组(CEP... 目的 探讨竖脊肌平面阻滞(ESP)对腹腔镜结直肠癌根治术患者细胞免疫功能的影响。方法 选择60例择期拟行腹腔镜结直肠癌根治术患者,采用随机数字表法分为3组:竖脊肌平面阻滞复合全凭静脉麻醉组(ESP组)、硬膜外阻滞复合全凭静脉麻醉组(CEP组)和腹横肌平面阻滞复合全凭静脉麻醉组(TAP组),每组20例。ESP组采用超声引导下双侧竖脊肌平面阻滞,CEP组采用硬膜外神经阻滞,TAP组采用超声引导下双侧腹横肌平面阻滞。于手术前1d清晨(T0)、手术结束离开麻醉复苏室前5min(T1)、术后24h(T2)、48h(T3)、72h(T4)、96h(T5)6个时点分别抽取静脉血5ml,应用流式细胞仪检测血液NK细胞数目、Th1/Th2比值,ELISA检测血清中干扰素(IFN)浓度;并观察患者T1~T3视觉模拟评分(VAS)。结果 在T1时点,ESP组和CEP组VAS评分明显低于TAP组(P<0.05),ESP组与CEP组相比差异无统计学意义(P >0.05);在T2、T3时点,ESP组患者VAS评分明显低于CEP组和TAP组(P<0.05)。与T0时点比较,各组患者T1~T5时点NK细胞数目、Th1/Th2比值和IFN浓度均降低,ESP组与CEP组相比较,差异无统计学意义(P >0.05),但与ESP组和CEP组分别相比,TAP组明显下降(P<0.05)。结论 竖脊肌平面阻滞复合全凭静脉麻醉与硬膜外麻醉阻滞复合全凭静脉麻醉同样对腹腔镜结直肠癌根治术患者细胞免疫功能影响较小。 展开更多
关键词 竖脊肌平面阻滞 结直肠 肿瘤 细胞免疫
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Adenoma and advanced neoplasia detection rates increase from 45 years of age 预览
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作者 David Karsenti Gaelle Tharsis +7 位作者 Pascal Burtin Franck Venezia Gilles Tordjman Agnès Gillet Joelle Samama Karine Nahon-Uzan Philippe Cattan Maryan Cavicchi 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第4期447-456,共10页
BACKGROUND Colonoscopy is considered a valid primary screening tool for colorectal cancer(CRC).The decreasing risk of CRC observed in patients undergoing colonoscopy is correlated with the adenoma detection rate(ADR).... BACKGROUND Colonoscopy is considered a valid primary screening tool for colorectal cancer(CRC).The decreasing risk of CRC observed in patients undergoing colonoscopy is correlated with the adenoma detection rate(ADR).Due to the fact that screening programs usually start from the age of 50,very few data are available on the risk of adenoma between 40 and 49 years.However,the incidence of CRC is increasing in young populations and it is not uncommon in routine practice to detect adenomas or even advanced neoplasia during colonoscopy in patients under 50 years.AIM To compare the ADR and advanced neoplasia detection rate(ANDR)according to age in a large series of patients during routine colonoscopy.METHODS All consecutive patients who were scheduled for colonoscopy were included.Exclusion criteria were as follows:patients scheduled for partial colonoscopy or interventional colonoscopy(for stent insertion or stenosis dilation).Colonoscopies were performed in our unit by a team of 30 gastroenterologists in 2016.We determined the ADR and ANDR in each age group in the whole population and in the population with an average risk of CRC(excluding patients with personal or family history of advanced adenoma or cancer).RESULTS 6027 colonoscopies were performed in patients with a median age of 57 years(range,15-96).The ADR and ANDR were 28.6%and 9.7%,respectively,in the whole population.When comparing patients aged 40-44(n=382)and 45-49 years(n=515),a strong increase in all parameters from 45 years was observed,with the ADR rising from 9.7%in patients aged 40-44 to 21.2%between 45 and 49(P<0.001)and the ANDR increasing from 3.1%in patients aged 40-44 to 6.4%in those aged 45-49 years(P<0.03).With regard to patients aged 50-54(n=849),a statistically significant increase in the ADR and ANDR was not observed between patients aged 45-49 and those aged 50-54 years.In the population with an average risk of CRC,the ADR and ANDR were still significantly higher in patients aged 45-49 compared with those aged 40-44 years.CONCLUSION This 展开更多
关键词 COLORECTAL cancer SCREENING ADENOMA detection rate COLONOSCOPY COHORT study
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PZR promotes metastasis of colorectal cancer through increasing FAK and Src phosphorylation
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作者 Dan Tan Wenpeng Zhang +2 位作者 Yu Tao Yesseyeva Galiya Mingliang Wang 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第4期356-364,共9页
Metastasis is the main cause of death in patients with colorectal cancer (CRC), but the molecular mechanism is not yet fully understood. Previous studies have shown that P zero-related protein (PZR), a member of the i... Metastasis is the main cause of death in patients with colorectal cancer (CRC), but the molecular mechanism is not yet fully understood. Previous studies have shown that P zero-related protein (PZR), a member of the imm uno globulin family, can promote fibr onectin-depe ndent migratio n of mouse embryonic fibroblasts as well as invasion and metastasis of hepatic carcinoma cells. However, the role of PZR in CRC remains unclear. In this study, we determined the ectopic expression of PZR in CRC tissues, and results showed that PZR expression was in creased not only in tumors with higher pathological stage, but also in tumors with distant metastasis. Through PZRknockdown and overexpression in CRC cell lines, we found that the expression of PZR had significant effect on the invasion and migration of CRC cells as well as the phosphorylation of prometastasis proteins including focal adhesion kinase (FAK) and Src. Taken together, this study indicates that PZR may promote the invasion and migration of CRC cells through increasing the phosphorylation of FAK and Src, which provides a new theoretical basis and a possible marker for the diag nosis or prog nosis of CRC metastasis. 展开更多
关键词 colorectal cancer PZR METASTASIS FAK SRC
Clinical assessment and identification of immuno-oncology markers concerning the 19-gene based risk classifier in stage Ⅳ colorectal cancer 预览
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作者 Jong Lyul Lee Seon Ae Roh +7 位作者 Chan Wook Kim Yi Hong Kwon Ye Jin Ha Seon-Kyu Kim Seon-Young Kim Dong-Hyung Cho Yong Sung Kim Jin Cheon Kim 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第11期1341-1354,共14页
BACKGROUND Genomic profiling of tumors has contributed to the understanding of colorectal cancer(CRC), facilitating diagnosis, prognosis and selection of treatments,including targeted regimens. A report suggested that... BACKGROUND Genomic profiling of tumors has contributed to the understanding of colorectal cancer(CRC), facilitating diagnosis, prognosis and selection of treatments,including targeted regimens. A report suggested that a 19-gene-based risk classifier(TCA19) was a prognostic tool for patients with stage III CRC. The survival outcomes in patients with stage IV CRC are still poor and appropriate selection of targeted therapies and immunotherapies is challenging.AIM To assess clinical implication of TCA19 in patients with stage IV CRC, and to identify TCA19 with involvement in immune-oncology.METHODS A retrospective review of the medical records of 60 patients with stage IV CRC was conducted, assessing clinicopathological variables and progression-free survival(PFS). TCA19 gene expression was determined by quantitative polymerase chain reaction(qPCR) in matched normal and tumor tissues taken from the study cohort. Expression of potential immune-oncology regulatory proteins and targets was examined by immunohistochemistry(IHC), western blot, immunofluorescence staining in tissues from a validation cohort of 10 patients, and in CRC cell lines co-cultured with monocyte in vitro.RESULTS In the patients with TCA19 score higher than the median, the PFS rates of eight patients who received the targeted regimens were significantly higher than the PFS rates of four patients who received 5-fluorouracil-based regimen(P = 0.041).In multivariate analysis, expression of signaling lymphocytic activation molecule family, member 7(SLAMF7) and triggering receptor expressed on myeloid cells 1(TREM1) was associated with PFS in the 60-patient cohort. After checking another 10 validate set, the expression of the IHC, the level of real-time qPCR,and the level of western blot were lower for SLAMF7 and higher for TREM7 in primary and metastatic tumors than in normal tissues. In CRC cells expressing SLAMF7 that were co-cultured with a monocytic cell line, levels of CD 68 and CD73 were significantly lower at day 5 of co-culture than at day 0.CO 展开更多
关键词 Colorectal cancer Prognosis Immunotherapy Signaling LYMPHOCYTIC activation molecule family member 7 TRIGGERING receptor expressed on MYELOID cells 1
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Diagnostic Value of 128-slice Spiral CT Combined with Virtual Colonoscopy for Colorectal Cancer 预览
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作者 Wen-juan TANG Zhuang NIE +3 位作者 Wen-liang FAN Lan CHENG Zi-qiao LEI Ming YANG 《当代医学科学(英文)》 SCIE CAS 2019年第1期146-152,共7页
The objective of this study was to evaluate the diagnostic value of 128-slice spiral CT combined with virtual colonoscopy in diagnosis of colorectal cancer.We retrospectively analyzed 45 patients of colorectal disease... The objective of this study was to evaluate the diagnostic value of 128-slice spiral CT combined with virtual colonoscopy in diagnosis of colorectal cancer.We retrospectively analyzed 45 patients of colorectal diseases who underwent definition AS+128-slice spiral CT combined with virtual colonoscopy after bowel preparation and gas injection to evaluate the clinical diagnostic value of this technology.All the patients received electronic colonoscopy and were confirmed by pathology.In total,colorectal cancer was confirmed in 42 cases and inflammation in 3 cases.Diagnostic results shows:there were 17 cases of lump,10 cases of infiltration,6 cases of ulcer,9 cases of mixed type,4 cases of liver metastases,and 36 cases of lymph node metastasis.There was no significant difference between 128-slice spiral CT combined with virtual colonoscopy and electronic colonoscopy in detection,localization and characterization of colorectal tumors.CT virtual endoscopy has great advantages in observing the invasion around the lesion and the presence or absence of metastasis in distant organs and lymph node metastasis.It is also possible to understand the shape of the lesion in the intestinal lumen and the length of the lesion involving the lumen of the intestine. 展开更多
关键词 128-slice SPIRAL CT VIRTUAL ENDOSCOPY COLORECTAL cancer
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TIGAR knockdown enhanced the anticancer effect of aescin via regulating autophagy and apoptosis in colorectal cancer cells
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作者 Bin Li Zhong Wang +8 位作者 Jia-ming Xie Gang Wang Li-qiang Qian Xue-mei Guan Xue-ping Shen Zheng-hong Qin Gen-hai Shen Xiao-qiang Li Quan-gen Gao 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第1期111-121,共11页
Our previous study showed that TP53-induced glycolysis and apoptosis regulator (TIGAR)regulated ROS,autophagy,and apoptosis in response to hypoxia and chemotherapeutic drugs.Aescin,a triterpene saponin,exerts anticanc... Our previous study showed that TP53-induced glycolysis and apoptosis regulator (TIGAR)regulated ROS,autophagy,and apoptosis in response to hypoxia and chemotherapeutic drugs.Aescin,a triterpene saponin,exerts anticancer effects and increases,ROS levels.The ROS is a key upstream signaling to activate autophagy.Whether there is a crosstalk between TIGAR and aescin in regulating ROS,autophagy,and apoptosis is unknown.In this study,we found that aescin inhibited cell viability and colony formation,and induced DNA damage,cell cycle arrest,and apoptosis in cancer cell lines HCT-116 and HCT-8 cells.Concurrently, aescin increased the expression of TIGAR,ROS levels,and autophagy activation.Knockdown of TIGAR enhanced the anticancer effects of aescin in vitro and in vivo,whereas overexpression of TIGAR or replenishing TIGAR downstream products,NADPH and ribose,attenuated aescin-induced apoptosis.Furthermore,aescin-induced ROS elevation and autophagy activation were further strengthened by TIGAR knockdown in HCT-116 cells.However,autophagy inhibition by knockdown of autophagy-related gene ATG5 or 3-methyladenine (3-MA)exaggerated aescin-induced apoptosis when TIGAR was knocked down.in conclusion,TIGAR plays a dual role in determining cancer cell fate via inhibiting both apoptosis and autophagy in response to aescin,which indicated that inhibition of TiGAR and/or~autophagy may be a junctional therapeutic target in treatment of cancers with aescin. 展开更多
关键词 TIGAR AESCIN APOPTOSIS AUTOPHAGY COLORECTAL cancer
mTOR-S6K1 pathway mediates cytoophidium assembly
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作者 Zhe Sun Ji-Long Liu 《遗传学报:英文版》 SCIE CAS CSCD 2019年第2期65-74,共10页
CTP synthase(CTPS), the rate-limiting enzyme in de novo CTP biosynthesis, has been demonstrated to assemble into evolutionarily conserved filamentous structures, termed cytoophidia, in Drosophila, bacteria, yeast and ... CTP synthase(CTPS), the rate-limiting enzyme in de novo CTP biosynthesis, has been demonstrated to assemble into evolutionarily conserved filamentous structures, termed cytoophidia, in Drosophila, bacteria, yeast and mammalian cells. However, the regulation and function of the cytoophidium remain elusive. Here, we provide evidence that the mechanistic target of rapamycin(mTOR) pathway controls cytoophidium assembly in mammalian and Drosophila cells. In mammalian cells, we find that inhibition of mTOR pathway attenuates cytoophidium formation. Moreover, CTPS cytoophidium assembly appears to be dependent on the mTOR complex 1(mTORC1) mainly. In addition, knockdown of the mTORC1 downstream target S6 K1 can inhibit cytoophidium formation, while overexpression of the constitutively active S6 K1 reverses mTOR knockdown-induced cytoophidium disassembly. Finally, reducing m TOR protein expression results in a decrease of the length of cytoophidium in Drosophila follicle cells.Therefore, our study connects CTPS cytoophidium formation with the mTOR signaling pathway. 展开更多
关键词 MTOR Cytoophidium CTP SYNTHASE COLORECTAL cancer cell DROSOPHILA
结直肠癌患者瘤组织中EGFR、COX-2表达及其临床意义 预览
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作者 谭志军 蒋宏 《世界最新医学信息文摘(电子版)》 2019年第17期11-12,共2页
目的考察结直肠癌患者瘤组织中EGFR、COX-2表达及其临床意义。方法以2013至2016年在我院接受手术治疗的结直肠癌患者为研究对象,收集患者临床资料,采用免疫组化法分析肿瘤样本中EGFR、COX-2表达水平,卡方检验比较EGFR、COX-2表达与患者... 目的考察结直肠癌患者瘤组织中EGFR、COX-2表达及其临床意义。方法以2013至2016年在我院接受手术治疗的结直肠癌患者为研究对象,收集患者临床资料,采用免疫组化法分析肿瘤样本中EGFR、COX-2表达水平,卡方检验比较EGFR、COX-2表达与患者临床病理特征的关系。结果 EGFR表达与患者瘤径(P<0.05)、N分期显著相关(P<0.05),COX-2表达与患者Dukes分期(P<0.01)、N分期(P<0.05)、肿瘤分级显著相关(P<0.01);EGFR表达与COX-2表达显著相关(P<0.05)。结论肿瘤组织中EGFR和COX-2的表达与结直肠癌瘤组织的病理学特征显著相关,可能与结直肠癌分化和转移有关。 展开更多
关键词 结直肠 肿瘤 临床
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Prognostic significance of PD-L1 expression in patients with colorectal cancer: a meta-analysis 预览
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作者 Siwen Liu Rong Ma +7 位作者 Haixia Cao Dan Chen Changwen Jing Zhuo Wang Junying Zhang Yang Wu Jifeng Feng Jianzhong Wu 《肿瘤学与转化医学:英文版》 2019年第2期68-74,共7页
Background The association between the expression of programmed cell death 1(PD-1) or its ligand [programmed cell death ligand-1(PD-L1)] and colorectal cancer(CRC) survival rates remains unclear. Thus, we conducted a ... Background The association between the expression of programmed cell death 1(PD-1) or its ligand [programmed cell death ligand-1(PD-L1)] and colorectal cancer(CRC) survival rates remains unclear. Thus, we conducted a meta-analysis to investigate the prognostic value of PD-L1 expression in CRC patients.Methods All eligible studies related to evaluation of PD-L1 expression and survival of CRC patients were searched in PubMed, Medline, Cochrane library, and the EMBASE database. Hazard ratios(HRs) and 95% confidence intervals(CI) of overall survival(OS) were examined to assess the effect of PD-L1 expression on the survival of CRC patients. The outcomes of this meta-analysis were synthesized based on randomeffects model. Subgroup analyses were also performed. Results Seven studies, wherein OS data were stratified according to the expression status of PD-L1, were analyzed. CRC patients showing positive PD-L1 expression were associated with significantly poorer prognoses in terms of overall survival, compared with those displaying negative PD-L1 expression(HR = 1.43, 95% CI: 1.07–1.92;P = 0.02). In the subgroup analyses, H-scores as well as the percentage of stained cells indicated that PD-L1 expression was significantly associated with poor prognosis(HR = 1.90, 95% CI: 1.38–2.62, P < 0.01;HR = 1.81, 95% CI: 1.08–3.03, P = 0.02). Immunohistochemical staining, utilizing a rabbit anti-PD-L1 antibody, revealed significantly superior survival in the PD-L1 negative group compared with the PD-L1 positive expression group(HR = 1.92;95% CI, 1.40-2.63;P < 0.01). Moreover, PD-L1 expression was significantly associated with poor prognosis when polyclonal antibodies were used(HR = 1.84;95% CI, 1.30–2.61;P < 0.01). Conclusion Our meta-analysis indicated that PD-L1 expression status is a significant prognostic factor for CRC patients. Positive PD-L1 expression was associated with worse CRC survival. Evaluation via different immunohistochemistry based techniques may partly account for the contradictory results. Therefore, furt 展开更多
关键词 COLORECTAL cancer (CRC) programmed cell DEATH ligand-1 (PD-L1) prognosis META-ANALYSIS
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Safety and efficacy of EFGR and VEGF signaling pathway inhibition therapy in patients with colorectal cancer: a meta-analysis 预览
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作者 Siwen Liu Rong Ma +7 位作者 Haixia Cao Shaorong Yu Dan Chen Changwen Jing Zhuo Wang Junying Zhang Jifeng Feng Jianzhong Wu 《肿瘤学与转化医学:英文版》 2019年第2期80-90,共11页
Objective Epidermal growth factor receptor(EGFR) and vascular endothelial growth factor(VEGF) inhibitors are two targeted therapies for metastatic colorectal cancer(mCRC). However, few studies have focused on the safe... Objective Epidermal growth factor receptor(EGFR) and vascular endothelial growth factor(VEGF) inhibitors are two targeted therapies for metastatic colorectal cancer(mCRC). However, few studies have focused on the safety and efficacy of combined targeted therapy against those of a single inhibition therapy of EFGR or VEGF. This meta-analysis aimed to compare the anti-tumor activity of the combined inhibition therapy and single inhibition therapy in patients with mCRC. Methods We searched PubMed, Medline, the Cochrane library, Embase, and annual meeting proceedings for relevant clinical trials. Objective response rate(ORR), progression-free survival(PFS), overall survival(OS), and adverse events were extracted and calculated.Results Nine trials comprising 3977 patients were selected for the analysis. The combined inhibition therapy showed a 3.7% improvement in ORR compared with single inhibition, and this difference was statistically significant [hazard ratio(HR)= 1.33;95% confidence interval(CI), 1.01–1.74;P = 0.04]. Subgroup analysis showed that the combined EGFR and VEGF inhibitor therapy had an 11.65% improvement in ORR compared with VEGF inhibitor therapy(OR = 2.14;95% CI, 1.34–3.40;P = 0.001). EGFR and VEGF inhibitor therapy and chemotherapy had an 18.08% improvement in ORR compared with chemotherapy(OR = 2.21;95% CI, 1.05–4.64;P = 0.04). Moreover, EGFR and VEGF inhibitor therapy significantly improved PFS compared with VEGF inhibitor therapy(OR = 0.82;95% CI, 0.69–0.97;P = 0.02). VEGF inhibitor therapy and chemotherapy significantly improved PFS compared with EGFR and VEGF inhibitor therapy and chemotherapy(OR = 1.20;95% CI, 1.11–1.30;P = 0.00). In addition, EGFR and VEGF inhibitor therapy showed improved OS compared with VEGF inhibitor therapy(HR = 0.78, 95% CI: 0.65–0.94;P = 0.008). Finally, the combined inhibition therapy showed an obviously increased risk of cutaneous and mucosal effects(RR = 6.45;95% CI: 2.71–15.36;P < 0.01), diarrhea/abdominal pain(RR = 1.97;95% CI: 1.45–2.68;P < 0.01), 展开更多
关键词 colorectal cancer (CRC) EPIDERMAL GROWTH FACTOR receptor (EGFR) vascular endothelial GROWTH FACTOR (VEGF) META-ANALYSIS
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Folate-targeted nanostructured chitosan/chondroitin sulfate complex carriers for enhanced delivery of bortezomib to colorectal cancer cells 预览
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作者 Zar Chi Soe Bijay Kumar Poudel +10 位作者 Hanh Thuy Nguyen Raj Kumar Thapa Wenquan Ou Milan Gautam Kishwor Poudel Sung Giu Jin Jee-Heon Jeong Sae Kwang Ku Han-Gon Choi Chul Soon Yong Jong Oh Kim 《亚洲药物制剂科学(英文)》 2019年第1期40-51,共12页
Folate-targeting self-assembled nanoparticles(NPs) using biocompatible and biodegradable natural polymers chitosan(Cs) and chondroitin sulfate(Chs) were developed to address the major challenge in cancer treatment, th... Folate-targeting self-assembled nanoparticles(NPs) using biocompatible and biodegradable natural polymers chitosan(Cs) and chondroitin sulfate(Chs) were developed to address the major challenge in cancer treatment, the selective delivery of nanoparticles to the target site. In this study, we successfully incorporated a hydrophobic drug, bortezomib(Bor), into folic acid(FA)-conjugated Cs/Chs self-assembled NPs(Bor/Cs/Chs-FA) for colorectal cancer therapy. The particle size and polydispersity index of Bor/Cs/Chs-FA were ~196.5 ± 1.2 nm and ~0.21 ± 0.5, respectively. A pH-dependent release profile was observed, facilitating cancer cell-targeted drug release under an acidic tumor microenvironment. Moreover, in vitro data revealed enhanced cellular uptake and apoptosis in folate receptor-expressing colorectal cancer cells(HCT-116 and HT-29) as compared to that in lung cancer cells(A549), which do not express folate receptors. Furthermore, intravenous administration of Bor/Cs/Chs-FA in a HCT-116 bearing xenograft mouse model showed that the NPs were a safe and effective drug delivery system. The results suggest that folate-targeted nanoparticle can be effectively applied for efficient chemotherapy of colorectal cancer. 展开更多
关键词 BORTEZOMIB CHITOSAN CHONDROITIN SULFATE COLORECTAL cancer Folic acid
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Human colorectal cancer cells frequently express IgG and display unique Ig repertoire 预览
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作者 Zi-Han Geng Chun-Xiang Ye +6 位作者 Yan Huang Hong-Peng Jiang Ying-Jiang Ye Shan Wang Yuan Zhou Zhan-Long Shen Xiao-Yan Qiu 《世界胃肠肿瘤学杂志:英文版(电子版)》 CAS 2019年第3期195-207,共13页
BACKGROUND There is growing evidence proving that many human carcinomas, including colon cancer, can overexpress immunoglobulin(Ig);the non B cancer cell-derived Ig usually displayed unique V(D)J rearrangement pattern... BACKGROUND There is growing evidence proving that many human carcinomas, including colon cancer, can overexpress immunoglobulin(Ig);the non B cancer cell-derived Ig usually displayed unique V(D)J rearrangement pattern that are distinct from B cell-derived Ig. Especially, the cancer-derived Ig plays important roles in cancer initiation, progression, and metastasis. However, it still remains unclear if the colon cancer-derived Ig can display unique V(D)J pattern and sequencing, which can be used as novel target for colon cancer therapy.AIM To investigate the Ig repertoire features expressed in human colon cancer cells.METHODS Seven cancerous tissue samples of colon adenocarcinoma and corresponding noncancerous tissue samples were sorted by fluorescence-activated cell sorting using epithelial cell adhesion molecule as a marker for epithelial cells. Ig repertoire sequencing was used to analyze the expression profiles of all 5 classes of Ig heavy chains(IgH) and the Ig repertoire in colon cancer cells and corresponding normal epithelial cells.RESULTS We found that all 5 IgH classes can be expressed in both colon cancer cells and normal epithelial cells. Surprisingly, unlike the normal colonic epithelial cells that expressed 5 Ig classes, our results suggested that cancer cells most prominently express IgG. Next, we found that the usage of Ig in cancer cells caused the expression of some unique Ig repertoires compared to normal cells. Some VH segments, such as VH3-7, have been used in cancer cells, and VH3-74 was frequently present in normal epithelial cells. Moreover, compared to the normal cell-derived Ig, most cancer cell-derived Ig showed unique VHDJH patterns.Importantly, even if the same VHDJH pattern was seen in cancer cells and normal cells, cancer cell-derived IgH always displayed distinct hypermutation hot points.CONCLUSION We found that colon cancer cells could frequently express IgG and unique IgH repertoires, which may be involved in carcinogenesis of colon cancer. The unique IgH repertoire has the potenti 展开更多
关键词 IMMUNOGLOBULIN REPERTOIRE SEQUENCING COLORECTAL cancer VDJ PATTERN VJ PATTERN
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Colorectal endoscopic submucosal dissection in special locations 预览
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作者 Uayporn Kaosombatwattana Takeshi Yamamura +2 位作者 Masanao Nakamura Yoshiki Hirooka Hidemi Goto 《世界胃肠内镜杂志:英文版(电子版)》 2019年第4期262-270,共9页
Colorectal endoscopic submucosal dissection (ESD) is considered one of the most challenging endoscopic procedures for novice endoscopists. When compared with the stomach, the colon and rectum have a narrower tubular l... Colorectal endoscopic submucosal dissection (ESD) is considered one of the most challenging endoscopic procedures for novice endoscopists. When compared with the stomach, the colon and rectum have a narrower tubular lumen, greater angulation at the flexures, and a thinner muscle layer. These factors make endoscopic control and maneuverability difficult. ESD of the colorectum was considered more difficult than gastric and esophageal ESD. However, with learning from the experts, practicing, and selecting an appropriate technique, most of colorectal ESD could be performed successfully. Nevertheless, some colorectal locations are extremely specialized either from unique anatomy or given unstable scope position. Accordingly, the objective of this review was to provide endoscopists with an overview of the techniques and outcomes associated with ESD at these special colorectal locations. ESD at the discussed special locations of the ileo-colo-rectum was found to be feasible, and outcomes were comparable to those of ESD performed in non-special locations of the ileocolo- rectum. Practice for skill improvement and awareness of the unique characteristics of each special location is the key to performing successful ESD. 展开更多
关键词 COLORECTAL ENDOSCOPIC SUBMUCOSAL DISSECTION ENDOSCOPIC SUBMUCOSAL DISSECTION SPECIAL LOCATIONS
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