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Hydrogel-based local drug delivery strategies for spinal cord repair 认领
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作者 Robert B.Shultz Yinghui Zhong 《中国神经再生研究:英文版》 SCIE CAS 2021年第2期247-253,共7页
Spinal cord injury results in significant loss of motor, sensory, and autonomic functions. Although a wide range of therapeutic agents have been shown to attenuate secondary injury or promote regeneration/repair in an... Spinal cord injury results in significant loss of motor, sensory, and autonomic functions. Although a wide range of therapeutic agents have been shown to attenuate secondary injury or promote regeneration/repair in animal models of spinal cord injury, clinical translation of these strategies has been limited, in part due to difficulty in safely and effectively achieving therapeutic concentrations in the injured spinal cord tissue. Hydrogelbased drug delivery systems offer unique opportunities to locally deliver drugs to the injured spinal cord with sufficient dose and duration, while avoiding deleterious side effects associated with systemic drug administration. Such local drug delivery systems can be readily fabricated from biocompatible and biodegradable materials. In this review, hydrogel-based strategies for local drug delivery to the injured spinal cord are extensively reviewed, and recommendations are made for implementation. 展开更多
关键词 drug carriers drug delivery HYDROGELS MICROPARTICLES nanoparticles neurotrophic factors scaffolds spinal cord injury
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Systematic review on the adverse reactions of oral administration of Indigo Naturalis and its preparations 认领
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作者 Xueyi Deng Rui Lan +7 位作者 Qianwen Xie Jingmin Xiao Jiaqi Lai Jing Chen Yihan He Shaonan Liu Lihong Yang Xinfeng Guo 《TMR现代中药》 2021年第1期34-43,共10页
Objective:This article systematically analyses the effects of adverse drug events/adverse drug reactions(ADEs/ADRs)of oral Indigo Naturalis(Qingdai)preparations in order to provide references for its rational clinical... Objective:This article systematically analyses the effects of adverse drug events/adverse drug reactions(ADEs/ADRs)of oral Indigo Naturalis(Qingdai)preparations in order to provide references for its rational clinical application.Methods:All clinical studies reporting ADE/ADR related to the oral administration of Qingdai preparations were searched through electronic databases,including PubMed,the Cochrane Library,Embase,China National Knowledge Infrastructure(CNKI),China Biology Medicine disc(CBM),VIP Information Chinese Journal Service Platform(VIP),and Wanfang database,from inception to September 27,2020.Information were extracted from these literatures,including primary disease,type of adverse reactions,dose,treatment,outcomes and so on.Incidence of ADE/ADR was estimated,as well as distribution of primary diseases and victim organs and systems were analyzed.Results:A total of 682 articles were included,with 651 clinical population studies and 31 case reports.Among them,604 detailed ADR/AE involving 33459 patients using oral Qingdai preparations,and a total of 5061 cases were found to present adverse events,including 2827 cases of digestive system(abdominal pain,diarrhea,etc.),469 cases of blood system damage(thrombocytopenia,leukopenia,anemia,etc.),313 cases of liver damage(abnormal liver function,liver toxicity,elevated liver enzymes,etc.),327 cases of nervous system reactions(headache,dizziness,poor sleep,etc.)and 1186 cases of other systems and organs.Severe adverse events(SAEs)mainly were liver damage,and could be relived after symptomatic treatment.Conclusion:From the systematic information retrieval and analysis,it is found that oral Qingdai preparations application may clinically cause ADEs/ADRs in terms of gastrointestinal tract and liver damage.Therefore,when using oral Qingdai preparations,liver and stomach protection should be done.At the same time,pay close attention to various biochemical indicators and the patient's drug response during the treatment process,and,if necessary,deal with it in time 展开更多
关键词 Qingdai Indigo Naturalis Oral preparations Adverse drug reactions Adverse drug events Systematic review
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Novel glucose-lowering drugs for non-alcoholic fatty liver disease 认领
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作者 Zuo-Di Fu Xiao-Ling Cai +3 位作者 Wen-Jia Yang Ming-Ming Zhao Ran Li Yu-Feng Li 《世界糖尿病杂志:英文版(电子版)》 SCIE CAS 2021年第1期84-97,共14页
BACKGROUND The efficacy of novel glucose-lowering drugs in treating non-alcoholic fatty liver disease(NAFLD)is unknown.AIM To evaluate the efficacy of glucose-lowering drugs dipeptidyl peptidase-4(DPP-4)inhibitors,glu... BACKGROUND The efficacy of novel glucose-lowering drugs in treating non-alcoholic fatty liver disease(NAFLD)is unknown.AIM To evaluate the efficacy of glucose-lowering drugs dipeptidyl peptidase-4(DPP-4)inhibitors,glucagon-like peptide-1 receptor agonists(GLP-1 RAs),and sodiumglucose cotransporter 2(SGLT2)inhibitors in treating NAFLD and to perform a comparison between these treatments.METHODS Electronic databases were systematically searched.The inclusion criteria were:Randomized controlled trials comparing DPP-4 inhibitors,GLP-1 RAs,or SGLT2 inhibitors against placebo or other active glucose-lowering drugs in NAFLD patients,with outcomes of changes in liver enzyme[alanine aminotransferase(ALT)and/or aspartate aminotransferase(AST)]from baseline.RESULTS Nineteen studies were finally included in this meta-analysis.Compared with placebo or other active glucose-lowering drug treatment,treatment with DPP-4 inhibitors,GLP-1 RAs,and SGLT2 inhibitors all led to a significant decrease in ALT change and AST change from baseline.The difference between the DPP-4 inhibitor and SGLT2 inhibitor groups in ALT change was significant in favor of DPP-4 inhibitor treatment(P<0.05).The trends of reduction in magnetic resonance imaging proton density fat fraction and visceral fat area changes were also observed in all the novel glucose-lowering agent treatment groups.CONCLUSION Treatment with DPP-4 inhibitors,GLP-1 RAs,and SGLT2 inhibitors resulted in improvements in serum ALT and AST levels and body fat composition,indicating a beneficial effect in improving liver injury and reducing liver fat in NAFLD patients. 展开更多
关键词 Non-alcoholic fatty liver disease Glucose-lowering drug META-ANALYSIS Dipeptidyl peptidase-4 inhibitor Sodium-glucose cotransporter 2 inhibitor Glucagon-like peptide-1 receptor agonist
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Triolein emulsion infusion into the hepatic artery increases vascular permeability to doxorubicin in rabbit liver 认领
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作者 Yong-Woo Kim Hak Jin Kim +1 位作者 Byung Mann Cho Seon Hee Choi 《世界胃肠病学杂志:英文版》 SCIE CAS 2021年第2期152-161,共10页
BACKGROUND The infusion of triolein emulsion(TE)induced increased vascular permeability and a negligible and temporary decrease in liver function without specific histopathological damage.AIM To assess changes in doxo... BACKGROUND The infusion of triolein emulsion(TE)induced increased vascular permeability and a negligible and temporary decrease in liver function without specific histopathological damage.AIM To assess changes in doxorubicin concentration according to the percentage of TE infused via a hepatic artery to study the vascular permeability in the rabbit liver.METHODS Thirty-nine healthy rabbits were divided into five groups according to the concentration of emulsified triolein infused into the hepatic arteries:Group 0,saline infusion(control group,n=5);group 1,0.3%TE(n=13);group 2,0.6%TE(n=6);group 3,0.9%TE(n=8);and group 4,1.5%TE(n=6).Doxorubicin(2.4 mg/kg)was infused immediately after TE injection via the hepatic arteries.After 2 h,the livers were harvested,and doxorubicin concentrations were calculated fluorometrically.The doxorubicin concentrations were compared between TE groups and the control group,and the optimal concentrations within the TE groups were calculated.Statistical analysis was performed using the nonparametric Mann-Whitney U test and Kruskal-Wallis test.P<0.05 were considered statistically significant.RESULTS In the liver,doxorubicin concentrations were 2.06,2.07,2.16 and 1.66 times higher in groups 1 through 4,respectively,and significantly higher in the TE groups than in the control group(all P<0.05).However,there were no significant differences in the mean doxorubicin concentrations between the four TE groups(P=0.642).In the lungs,the mean doxorubicin concentrations were not significantly different between the control and TE groups(P>0.05).CONCLUSION TE infusion into the hepatic arteries significantly increased the doxorubicin concentration approximately twofold but was not different between the TE groups.These findings suggest that TE infusion might be a useful adjuvant treatment of liver cancers. 展开更多
关键词 Triolein emulsion Liver tumor CHEMOTHERAPY Drug delivery Vascular permeability Dexorubicin
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Artificial intelligence and the future of medicine: a multidimensional analysis 认领
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作者 Philippe Moingeon 《TMR生命研究》 2021年第1期59-66,共8页
This multidimensional analysis of the impact of artificial intelligence on the future of medicine aims to give some clues on foreseen categories of applications as well as their societal implications in terms of risks... This multidimensional analysis of the impact of artificial intelligence on the future of medicine aims to give some clues on foreseen categories of applications as well as their societal implications in terms of risks/benefits.Artificial intelligence encompasses technologies recapitulating four dimensions of human intelligence,i.e.sensing,thinking,acting and learning.Intelligent machines are converging with advancing biotechnologies to shape the future of medicine,in synergy with continuous progress in our understanding of system biology,brain physiology,biology of aging,computational sciences and decision-making theories.Data-driven predictive models of health-related problems can be generated to inform decisions and actions,allowing to enhance productivity in new drug development,increase the cost-effectiveness of fully integrated health care systems and empower patients and healthy individuals to better manage their disease or their health,respectively.Consequently,the future will likely take the form of a computational precision medicine continuously informed by data capture and modeling to propose preventive measures or therapies precisely tailored to characteristics of each individual. 展开更多
关键词 Artificial intelligence Biotechnologies Computational medicine Disease model Drug development Patient empowerment Precision medicine
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Conferring receptors on recipient cells with extracellular vesicles for targeted drug delivery 认领
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作者 Zachary Quinn Wenjun Mao +2 位作者 Yiqiu Xia Rhea John Yuan Wan 《生物活性材料》 SCIE 2021年第3期749-756,共8页
Triple negative breast cancer(TNBC)is a heterogeneous subset of breast cancer characterized by its lack of estrogen receptor,progesterone receptor,and human epidermal growth factor receptor 2(HER2),which altogether pr... Triple negative breast cancer(TNBC)is a heterogeneous subset of breast cancer characterized by its lack of estrogen receptor,progesterone receptor,and human epidermal growth factor receptor 2(HER2),which altogether prevents TNBC from being treated effectively.For many years,the treatment paradigms and overall survival of patients with TNBC have remained largely stagnant.Recent attempts to convert cold tumors to hot tumors by promoting antigen presentation have shown increased T cell infiltration and significantly induced immune responses for tumor killing.Inspired by this concept,the expression of specific targetable antigens on TNBC cells may further benefit relevant targeted drug delivery.In this study,we successfully conferred sufficient HER2 on the surface of TNBC MDA-MB-231 cells via simple EV-plasma membrane fusion with HER2+extracellular vesicles(EV)derived from HER2 overexpressing BT-474 cells.Subsequently,anti-HER2 antibody conjugated paclitaxel-loaded liposomes were used for HER2-targeted drug delivery.Our findings demonstrated this HER2 grafting,in conjunction with targeted drug delivery,can improve the treatment efficacy in vitro and in vivo.This novel approach represents a facile method of altering cell membrane antigen presentation via convenient EVs uptake and may pave the way for the burgeoning wave of targeted therapy and/or immunotherapy. 展开更多
关键词 Extracellular vesicles Drug delivery Triple-negative breast cancer HER2
The role of peptidase neurolysin in neuroprotection and neural repair after stroke 认领 被引量:1
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作者 Vardan T.Karamyan 《中国神经再生研究:英文版》 SCIE CAS 2021年第1期21-25,共5页
Current experimental stroke research has evolved to focus on detailed understanding of the brain’s self-protective and restorative mechanisms,and harness this knowledge for development of new therapies.In this contex... Current experimental stroke research has evolved to focus on detailed understanding of the brain’s self-protective and restorative mechanisms,and harness this knowledge for development of new therapies.In this context,the role of peptidases and neuropeptides is of growing interest.In this focused review,peptidase neurolysin(Nln)and its extracellular peptide substrates are briefly discussed in relation to pathophysiology of ischemic stroke.Upregulation of Nln following stroke is viewed as a compensatory cerebroprotective mechanism in the acute phase of stroke,because the main neuropeptides inactivated by Nln are neuro/cerebrotoxic(bradykinin,substance P,neurotensin,angiotensin II,hemopressin),whereas the peptides generated by Nln are neuro/cerebroprotective(angiotensin-(1–7),Leu-/Met-enkephalins).This notion is confirmed by experimental studies documenting aggravation of stroke outcomes in mice after inhibition of Nln following stroke,and dramatic improvement of stroke outcomes in mice overexpressing Nln in the brain.The role of Nln in the(sub)chronic phase of stroke is less clear and it is likely,that this peptidase does not have a major role in neural repair mechanisms.This is because,the substrates of Nln are less uniform in modulating neurorestorative mechanisms in one direction,some appearing to have neural repair enhancing/stimulating potential,whereas others doing the opposite.Future studies focusing on the role of Nln in pathophysiology of stroke should determine its potential as a cerebroprotective target for stroke therapy,because its unique ability to modulate multiple neuropeptide systems critically involved in brain injury mechanisms is likely advantageous over modulation of one pathogenic pathway for stroke pharmacotherapy. 展开更多
关键词 compensatory cerebroprotection drug target endogenous neuroprotective mechanism NEUROPEPTIDE neurorestoration PEPTIDASE
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Functional recovery after peripheral nerve injury via sustained growth factor delivery from mineral-coated microparticles 认领
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作者 Daniel J.Hellenbrand Clayton L.Haldeman +9 位作者 Jae-Sung Lee Angela G.Gableman Elena K.Dai Stephen D.Ortmann Jerrod CGotchy Kierra K.Miller Adrianna M.Doucas Nicole C.Nowak William L.Murphy Amgad S.Hanna 《中国神经再生研究:英文版》 SCIE CAS 2021年第5期871-877,共7页
The gold standard for treating peripheral nerve injuries that have large nerve gaps where the nerves cannot be directly sutured back together because it creates tension on the nerve,is to incorporate an autologous ner... The gold standard for treating peripheral nerve injuries that have large nerve gaps where the nerves cannot be directly sutured back together because it creates tension on the nerve,is to incorporate an autologous nerve graft.However,even with the incorporation of a nerve graft,generally patients only regain a small portion of function in limbs affected by the injury.Although,there has been some promising results using growth factors to induce more axon growth through the nerve graft,many of these previous therapies are limited in their ability to release growth factors in a sustained manner and tailor them to a desired time frame.The ideal drug delivery platform would deliver growth factors at therapeutic levels for enough time to grow axons the entire length of the nerve graft.We hypothesized that mineral coated microparticles(MCMs)would bind,stabilize and release biologically active glial cell-derived neurotrophic factor(GDNF)and nerve growth factor(NGF)in a sustained manner.Therefore,the objective of this study was to test the ability of MCMs releasing growth factors at the distal end of a 10 mm sciatic nerve graft,to induce axon growth through the nerve graft and restore hind limb function.After sciatic nerve grafting in Lewis rats,the hind limb function was tested weekly by measuring the angle of the ankle at toe lift-off while walking down a track.Twelve weeks after grafting,the grafts were harvested and myelinated axons were analyzed proximal to the graft,in the center of the graft,and distal to the graft.Under physiological conditions in vitro,the MCMs delivered a burst release of NGF and GDNF for 3 days followed by a sustained release for at least 22 days.In vivo,MCMs releasing NGF and GDNF at the distal end of sciatic nerve grafts resulted in significantly more myelinated axons extending distal to the graft when compared to rats that received nerve grafts without growth factor treatment.The rats with nerve grafts incorporated with MCMs releasing NGF and GDNF also showed significant improvement in hind 展开更多
关键词 AUTOGRAFTS axon growth drug delivery glial cell-derived neurotrophic factor growth factors mineral coatings nerve grafting nerve growth factor
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阿司匹林生物学新作用及其对间充质干细胞功能影响的研究进展 认领
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作者 周颖 靳继德 《中国医药生物技术》 2021年第1期68-72,共5页
阿司匹林又称乙酰水杨酸(acctylsalicylic acid,ASA),是一种非甾体类抗炎药(non-steroidal anti-inflammatory drug,NSAIDs),其疗效稳定、副反应少,已广泛应用于临床,预防和治疗多种疾病,如风湿疾病、心脑血管疾病及糖尿病等。百余年来... 阿司匹林又称乙酰水杨酸(acctylsalicylic acid,ASA),是一种非甾体类抗炎药(non-steroidal anti-inflammatory drug,NSAIDs),其疗效稳定、副反应少,已广泛应用于临床,预防和治疗多种疾病,如风湿疾病、心脑血管疾病及糖尿病等。百余年来,随着对阿司匹林研究的不断深入,发现阿司匹林参与了多种生物学途径,一些新的药理作用也不断被发现。 展开更多
关键词 非甾体类抗炎药 心脑血管疾病 间充质干细胞 阿司匹林 乙酰水杨酸 NSAIDS 多种疾病 drug
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Fabrication of a hydroxyapatite-PDMS microfluidic chip for bone-related cell culture and drug screening 认领
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作者 Qiangqiang Tang Xiaoyu Li +4 位作者 Chen Lai Lei Li Hongkai Wu Yingjun Wang Xuetao Shi 《生物活性材料》 SCIE 2021年第1期169-178,共10页
Bone is an important part of the human body structure and plays a vital role in human health.A microfluidic chip that can simulate the structure and function of bone will provide a platform for bone-related biomedical... Bone is an important part of the human body structure and plays a vital role in human health.A microfluidic chip that can simulate the structure and function of bone will provide a platform for bone-related biomedical research.Hydroxyapatite(HA),a bioactive ceramic material,has a similar structure and composition to bone mineralization products.In this study,we used HA as a microfluidic chip component to provide a highly bionic bone environment.HA substrates with different microchannel structures were printed by using ceramic stereolithography(SLA)technology,and the minimum trench width was 50μm.The HA substrate with microchannels was sealed by a thin polydimethylsiloxane(PDMS)layer to make a HA-PDMS microfluidic chip.Cell culture experiments demonstrated that compared with PDMS,HA was more conducive to the proliferation and osteogenic differentiation of the human foetal osteoblast cell line(hFOB).In addition,the concentration gradient of the model drug doxorubicin hydrochloride(DOX)was successfully generated on a Christmas tree structure HA-PDMS chip,and the half maximal inhibitory concentration(IC50)of DOX was determined.The findings of this study indicate that the HA-PDMS microfluidic chip has great potential in the field of high-throughput bonerelated drug screening and bone-related research. 展开更多
关键词 Hydroxyapatite(HA) Ceramic microfluidic chip Drug screening BIOMIMETIC Bone-on-a-chip
Nanoparticle‑Loaded Polarized‑Macrophages for Enhanced Tumor Targeting and Cell‑Chemotherapy 认领
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作者 Teng Hou Tianqi Wang +7 位作者 Weiwei Mu Rui Yang Shuang Liang Zipeng Zhang Shunli Fu Tong Gao Yongjun Liu Na Zhang 《纳微快报:英文版》 SCIE EI CAS 2021年第1期79-98,共20页
Cell therapy is a promising strategy for cancer therapy.However,its therapeutic efficiency remains limited due to the complex and immunosuppressive nature of tumor microenvironments.In this study,the“cell-chemotherap... Cell therapy is a promising strategy for cancer therapy.However,its therapeutic efficiency remains limited due to the complex and immunosuppressive nature of tumor microenvironments.In this study,the“cell-chemotherapy”strategy was presented to enhance antitumor efficacy.M1-type macrophages,which are therapeutic immune cells with both of immunotherapeutic ability and targeting ability,carried sorafenib(SF)-loaded lipid nanoparticles(M1/SLNPs)were developed.M1-type macrophages were used both as therapeutic tool to provide immunotherapy and as delivery vessel to target deliver SF to tumor tissues for chemotherapy simultaneously.M1-type macrophages were obtained by polarizing macrophages using lipopolysaccharide,and M1/SLNPs were obtained by incubating M1-type macrophages with SLNP.Tumor accumulation of M1/SLNP was increased compared with SLNP(p<0.01),which proved M1/SLNP could enhance tumor targeting of SF.An increased ratio of M1-type macrophages to M2-type macrophages,and the CD3^+CD4^+T cells and CD3^+CD8^+T cell quantities in tumor tissues after treatment with M1/SLNP indicated M1/SLNP could relieve the immunosuppressive tumor microenvironments.The tumor volumes in the M1/SLNP group were significantly smaller than those in the SLNP group(p<0.01),indicating M1/SLNP exhibited enhanced antitumor efficacy.Consequently,M1/SLNP showed great potential as a novel cellchemotherapeutic strategy combining both cell therapy and targeting chemotherapy. 展开更多
关键词 Polarized-macrophages Cell therapy Cell-mediated drug delivery CHEMOTHERAPY Lipid nanoparticles
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Delayed atomoxetine or fluoxetine treatment coupled with limited voluntary running promotes motor recovery in mice after ischemic stroke 认领
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作者 Faisal F.Alamri Abdullah Al Shoyaib +5 位作者 Nausheen Syeara Anisha Paul Srinidhi Jayaraman Serob T.Karamyan Thiruma V.Arumugam Vardan T.Karamyan 《中国神经再生研究:英文版》 SCIE CAS 2021年第7期1244-1251,共8页
Currently, there is an unmet need for treatments promoting post-stroke functional recovery.The aim of this study was to evaluate and compare the dose-dependent effect of delayed atomoxetine or fluoxetine therapy(start... Currently, there is an unmet need for treatments promoting post-stroke functional recovery.The aim of this study was to evaluate and compare the dose-dependent effect of delayed atomoxetine or fluoxetine therapy(starting on post-stroke day 5), coupled with limited physical exercise(2 hours daily voluntary wheel running;post-stroke days 9 to 42), on motor recovery of adult male mice after photothrombotic stroke.These drugs are selective norepinephrine or serotonin reuptake inhibitors indicated for disorders unrelated to stroke.The predetermined primary end-point for this study was motor function measured in two tasks of spontaneous motor behaviors in grid-walking and cylinder tests.Additionally, we quantified the running distance and speed throughout the study, the number of parvalbumin-positive neurons in the medial agranular cortex and infarct volumes.Both sensorimotor tests revealed that neither limited physical exercise nor a drug treatment alone significantly facilitated motor recovery in mice after stroke.However, combination of physical exercise with either of the drugs promoted restoration of motor function by day 42 post-stroke, with atomoxetine being a more potent drug.This was accompanied by a significant decrease in parvalbumin-positive inhibitory interneurons in the ipsilateral medial agranular cortex of mice with recovering motor function, while infarct volumes were comparable among experimental groups.If further validated in larger studies, our observations suggest that add-on atomoxetine or fluoxetine therapy coupled with limited, structured physical rehabilitation could offer therapeutic modality for stroke survivors who have difficulty to engage in early, high-intensity physiotherapy.Furthermore, in light of the recently completed Assessment o F Fluoxet INe In s Troke recover Y(AFFINITY) and Efficacy o F Fluoxetine-a randomis Ed Controlled Trial in Stroke(EFFECTS) trials, our observations call for newly designed studies where fluoxetine or atomoxetine pharmacotherapy is evaluated in combination w 展开更多
关键词 drug repurposing neural repair physical exercise PHYSIOTHERAPY post-stroke recovery pre-clinical trial FLAME trial selective norepinephrine reuptake inhibitor selective serotonin reuptake inhibitor stroke pharmacotherapy
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Saccharomyces cerevisiae in neuroscience:how unicellular organism helps to better understand prion protein? 认领
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作者 Takao Ishikawa 《中国神经再生研究:英文版》 SCIE CAS 2021年第3期489-495,共7页
The baker's yeast Saccharomyces(S.)cerevisiae is a single-celled eukaryotic model organism widely used in research on life sciences.Being a unicellular organism,S.cerevisiae has some evident limitations in applica... The baker's yeast Saccharomyces(S.)cerevisiae is a single-celled eukaryotic model organism widely used in research on life sciences.Being a unicellular organism,S.cerevisiae has some evident limitations in application to neuroscience.However,yeast prions are extensively studied and they are known to share some hallmarks with mammalian prion protein or other amyloidogenic proteins found in the pathogenesis of Alzheimer's,Parkinson's,or Huntington's diseases.Therefore,the yeast S.cerevisiae has been widely used for basic research on aggregation properties of proteins in cellulo and on their propagation.Recently,a yeast-based study revealed that some regions of mammalian prion protein and amyloidβ1–42 are capable of induction and propagation of yeast prions.It is one of the examples showing that evolutionarily distant organisms share common mechanisms underlying the structural conversion of prion proteins making yeast cells a useful system for studying mammalian prion protein.S.cerevisiae has also been used to design novel screening systems for anti-prion compounds from chemical libraries.Yeastbased assays are cheap in maintenance and safe for the researcher,making them a very good choice to perform preliminary screening before further characterization in systems engaging mammalian cells infected with prions.In this review,not only classical red/white colony assay but also yeast-based screening assays developed during last year are discussed.Computational analysis and research carried out using yeast prions force us to expect that prions are widely present in nature.Indeed,the last few years brought us several examples indicating that the mammalian prion protein is no more peculiar protein–it seems that a better understanding of prion proteins nature-wide may aid us with the treatment of prion diseases and other amyloid-related medical conditions. 展开更多
关键词 amyloid artificial prion baker's yeast budding yeast drug screening fusion protein neurodegenerative diseases prion protein yeast-based assay
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不同剂量氯胺酮对小鼠宫颈癌细胞凋亡影响 认领
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作者 尚晓宁 何世华 李万鹏 《中国药物与临床》 CAS 2021年第2期220-222,共3页
目的探讨不同剂量氯胺酮对小鼠宫颈癌细胞凋亡影响。方法小鼠宫颈癌细胞-U14细胞株随机分为3组,实验2组与实验1组分别采用终浓度为5 mmol/L与1 mmol/L的盐酸氯胺酮溶液处理,对照组用等体积的达氏修正伊氏培养基(DMEM)溶液进行处理。噻... 目的探讨不同剂量氯胺酮对小鼠宫颈癌细胞凋亡影响。方法小鼠宫颈癌细胞-U14细胞株随机分为3组,实验2组与实验1组分别采用终浓度为5 mmol/L与1 mmol/L的盐酸氯胺酮溶液处理,对照组用等体积的达氏修正伊氏培养基(DMEM)溶液进行处理。噻唑蓝溶液(MTT)法检测细胞增殖,流式细胞仪检测细胞凋亡,划痕试验检测细胞迁移,蛋白质印迹法检测蛋白表达。结果细胞处理后24 h与36 h,实验1组与实验2组的细胞增殖活性、细胞迁移指数显著低于对照组,细胞凋亡指数、胱天蛋白酶(casepase)-3蛋白相对表达水平显著高于对照组(P<0.05),实验2组与实验1组比较差异具有统计学意义(P<0.05)。结论不同剂量氯胺酮在小鼠宫颈癌细胞中的应用能促进细胞凋亡,诱发casepase-3蛋白的过表达,从而抑制细胞增殖与迁移。 展开更多
关键词 氯胺酮 剂量剂应关系 药物 宫颈肿瘤 细胞凋亡
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Dual-Wavelength Photosensitive Nano-in-Micro Scaffold Regulates Innate and Adaptive Immune Responses for Osteogenesis 认领
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作者 Qin Zhao Miusi Shi +8 位作者 Chengcheng Yin Zifan Zhao Jinglun Zhang Jinyang Wang Kailun Shen Lingling Zhang Hua Tang Yin Xiao Yufeng Zhang 《纳微快报:英文版》 SCIE EI CAS 2021年第2期124-143,共20页
The immune response of a biomaterial determines its osteoinductive effect.Although the mechanisms by which some immune cells promote regeneration have been revealed,the biomaterial-induced immune response is a dynamic... The immune response of a biomaterial determines its osteoinductive effect.Although the mechanisms by which some immune cells promote regeneration have been revealed,the biomaterial-induced immune response is a dynamic process involving multiple cells.Currently,it is challenging to accurately regulate the innate and adaptive immune responses to promote osteoinduction in biomaterials.Herein,we investigated the roles of macrophages and dendritic cells(DCs)during the osteoinduction of biphasic calcium phosphate(BCP)scaffolds.We found that osteoinductive BCP directed M2 macrophage polarization and inhibited DC maturation,resulting in low T cell response and efficient osteogenesis.Accordingly,a dual-targeting nano-in-micro scaffold(BCP loaded with gold nanocage,BCP-GNC)was designed to regulate the immune responses of macrophages and DCs.Through a dual-wavelength photosensitive switch,BCP-GNC releases interleukin-4 in the early stage of osteoinduction to target M2 macrophages and then releases dexamethasone in the later stage to target immature DCs,creating a desirable inflammatory environment for osteogenesis.This study demonstrates that biomaterials developed to have specific regulatory capacities for immune cells can be used to control the early inflammatory responses of implanted materials and induce osteogenesis. 展开更多
关键词 Gold nanocage Drug release Photocatalysis IMMUNOMODULATION OSTEOINDUCTION
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文章速递Application of Liquid Chromatography-High Resolution Time-of-flight Mass Spectrometry in the Detection of Raw Milk and Dairy Products 认领
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作者 Yi LI Luman HUO +5 位作者 Lixue DONG Xuesong WANG Litian ZHANG Ruihuan DU Aijun LI Lei WANG 《农业生物技术:英文版》 CAS 2021年第1期111-113,共3页
Milk and dairy products are more and more popular with consumers due to their various nutrients, and their quality and safety issues have always been concerned. Therefore, the development of rapid, accurate and simple... Milk and dairy products are more and more popular with consumers due to their various nutrients, and their quality and safety issues have always been concerned. Therefore, the development of rapid, accurate and simple screening techniques is of great significance. Liquid chromatography-high resolution time-of-flight mass spectrometry has high-resolution and high-throughput detection functions, and has gradually begun to be applied in the detection of milk and dairy products. This paper summarized the application of milk and dairy products in liquid chromatography-high resolution time-of-flight mass spectrometry, laying a foundation for the development of new methods. 展开更多
关键词 Milk and dairy products High resolution time-of-flight mass spectrometry Pesticide and veterinary drug residues
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文章速递肝细胞癌中bcl-2家族对化疗耐药的研究进展 认领
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作者 吴军 郑庆范 +1 位作者 盛基尧 姚小晓 《中国实验诊断学》 北大核心 2021年第1期129-134,共6页
在全球范围内,肝细胞癌(hepatocellular carcinoma,HCC)是癌症相关死亡第二大肿瘤,占90%[1]。其特点为病死率、致死率高,侵袭性强,对化疗药物敏感性低,易产生耐药性[2]。晚期HCC化疗方案主要得益于阿霉素(adriamycin,DOX),5-氟尿嘧啶(5-... 在全球范围内,肝细胞癌(hepatocellular carcinoma,HCC)是癌症相关死亡第二大肿瘤,占90%[1]。其特点为病死率、致死率高,侵袭性强,对化疗药物敏感性低,易产生耐药性[2]。晚期HCC化疗方案主要得益于阿霉素(adriamycin,DOX),5-氟尿嘧啶(5-Fluorouracil,5-FU)和铂类等药物。但是由于肝细胞癌的多药耐药性(Multi-drug Resistant,MDR)。 展开更多
关键词 化疗药物敏感性 化疗耐药 肝细胞癌 多药耐药性 铂类 侵袭性 drug 阿霉素
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Analysis of Data on Adverse Drug Events Reported to the Food and Drugs Administration of the United States of America 认领
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作者 Emmanuel M. Baah 《统计学期刊(英文)》 2020年第2期203-227,共25页
Background: The Spontaneous Reporting System (SRS) of the Food and Drugs Administration (FDA) of the United States of America (US), known as the FDA Adverse Event Reporting System (FAERS), is a mechanism for collectin... Background: The Spontaneous Reporting System (SRS) of the Food and Drugs Administration (FDA) of the United States of America (US), known as the FDA Adverse Event Reporting System (FAERS), is a mechanism for collecting information on safety concerns associated with the use of drugs for redress, as they are used on large scale. The data which is the subject of this paper came from the FAERS database. This paper reports on the analysis of data covering 2013 to 2018 period, but compares the observed trends in the variables during this period with that of the 2007 to 2012 period to ascertain whether the trends change over time;as this paper is, in a sense, a sequel to an earlier one with a similar title as this but covering the period 2007 to 2012. Objectives: The objectives of the study reported in this paper were to: i) explore the trends in the variables involved with the adverse events problem in the 2013 to 2018 period and compare these trends with that found in the study covering the 2007 to 2012 period;ii) determine whether or not the level of missing variable values in the 2013 to 2018 period is lower than, the same or higher than it was in the 2007 to 2012 period;iii) find out how the first twenty principal suspect drugs most cited to be involved in adverse events occurring during drug use in the 2013 to 2018 period compare with that of the 2007 to 2012 period. Methods: The Food and Drugs Administration (FDA) makes extracts from the FAERS database freely available to the public on quarterly basis. Fourteen (14) out of over fifty (50) variables contained in these extracts were reckoned to be connected with the objectives of the study and were examined using the tools of frequencies, proportions and averages, on account of the nature of the data. Results: For the period 2013 to 2018, adverse events reports submitted to the FDA (US) more than doubled (2.1 times), accounting for an annual average growth rate of 15.8 %, which is considerably lower than the annual average growth rate of 22.1% for the 2007 to 2012 展开更多
关键词 Drugs ADVERSE DRUG Events (ADEs) ADVERSE DRUG Reactions (ADRs) SPONTANEOUS REPORTING System (SRS)
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Threefold Increase in the Number of Drug Resistant TB Cases after Introduction of Universal Drug Susceptibility Testing: Experiences from Two South India Districts 认领
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作者 Sanath Kumar Gurram Krishnamurthy Sharath Burugina Nagaraja +6 位作者 Tanu Anand Karuna D. Sagili Cheluve Gowda Shailaja Basavaraj Poojar Srinath Satyanarayana 《结核病研究(英文)》 2020年第2期42-52,共11页
<b>Background</b><b>: </b>In India, tuberculosis (TB) is a major public health problem, and the advent of drug resistance TB (DR-TB) has worsened the situation. The Revised National TB Control ... <b>Background</b><b>: </b>In India, tuberculosis (TB) is a major public health problem, and the advent of drug resistance TB (DR-TB) has worsened the situation. The Revised National TB Control Programme (RNTCP) has introduced universal drug susceptibility testing (UDST) for all diagnosed TB cases in 2018. We conducted this study to know the advantage of implementing UDST when compared to selective testing existent in 2017 on key diagnostic cascade parameters and to identify the challenges in the implementation of UDST. <b>Methods</b><b>: </b>The study was conducted in two districts of Karnataka, India during January 2017-December 2018. The quantitative part consisted of before-and-after design and the qualitative part consisted of descriptive design.<b> Results</b><b>: </b>In 2017 (during selective testing/“before” period) out of the 2440 TB patients, 80 (3%) were diagnosed with Isoniazid and Rifampicin resistance patients;in contrast in 2018 (during UDST/“after” period) of the 5129 TB patients 258 (5%) were diagnosed with Isoniazid and Rifampicin resistance. However, the proportion of eligible patients tested for rifampicin resistance during the “after” period was 60% when compared to 100% during the “before” period and median turnaround time for testing was also longer during the “after” period when compared to the “before” period (32.5 days vs 27.5 days). Major reasons for these two gaps were found to be difficulties in collecting sputum specimens and transportation. <b>Conclusion</b><b>: </b>The rollout of UDST has led to a three-fold increase in a number of DR-TB cases detected in the region. There is a need for the programme to increase the proportion tested for DST by increasing the laboratory capacity and address the challenges in sputum collection and transportation. 展开更多
关键词 Operational Research Criteria C Line Probe Assay Universal Drug Susceptibility Testing Culture and Drug Susceptibility Testing
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Prescription Analysis and Risk Category Assessment of Drugs Prescribed during Pregnancy in a Tertiary Care Center as per USFDA Classification 认领
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作者 Kota Veena Bharathi Keche Yogendra Narayanrao +2 位作者 Prasanta Kumar Nayak Gaikwad Nitin Rewaram Dhaneria Suryaprakash 《美中医学:英文版》 2020年第3期102-107,共6页
Objective:To carry out prescription analysis and assign risk category as per USFDA(United States Food and Drug Administration)to prescribed drug during pregnancy in a tertiary health care center at Raipur,Chhattisgarh... Objective:To carry out prescription analysis and assign risk category as per USFDA(United States Food and Drug Administration)to prescribed drug during pregnancy in a tertiary health care center at Raipur,Chhattisgarh.Method:This cross-sectional study was carried out over the period of 2 months in obstetric OPD(outpatient department)at tertiary health care center Raipur.Pregnant woman of any trimester was recruited from ANC(antenatal clinic)after obtaining informed written consent.Important information collected was demography,gravida,parity,trimester of pregnancy,reason for visit to clinic,history of any chronic illness,names of drugs prescribed with their doses and frequency of administration.Prescription analysis was carried out and USFDA risk category had been assigned to each of the drug.Results:Total 340 drugs were prescribed for 75 patients.And 51.43%pregnant women were from 3rd trimester of pregnancy.Most of pregnant women(92.86%)reported to OPD for regular ANC checkup.Prescription analysis shows that average number of drugs prescribed during pregnancy was 4.52 per prescription.Average FDCs(fixed dose combinations)prescribed were 1.64 per prescription.Vitamins and minerals FDCs(82.4%)were commonly prescribed FDCs.As per USFDA risk category,prescribed drugs were A(73.83%),B(18.82%),C(7.06%)and D(0.29%)respectively.Antimicrobials were prescribed in 25%pregnant women and commonly prescribed antimicrobial was nitrofurantoin(13.33%)for urinary tract infection.Conclusions:More than four drugs are prescribed in pregnancy.Drugs required to specifically treat associated health problems belong to category B which can be considered safe in pregnancy.Different class of drug though not labelled indication(off-label)needs to be prescribed as per treatment guideline to prevent the pregnancy loss and prevent post-partum complications. 展开更多
关键词 Risk category USFDA Off label use of drug in pregnancy Drug prescribing during pregnancy Rational use of drug during pregnancy
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