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Mycorrhizal fungal community composition in seven orchid species inhabiting Song Mountain, Beijing, China
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作者 Yanhong Chen Yue Gao +3 位作者 Linli Song Zeyu Zhao Shunxing Guo Xiaoke Xing 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第6期838-847,共10页
Mycorrhizal fungi play an important role in the germination and growth of orchids essentially influencing their survival,abundance, and spatial distribution. In this study, we investigated the composition of the mycor... Mycorrhizal fungi play an important role in the germination and growth of orchids essentially influencing their survival,abundance, and spatial distribution. In this study, we investigated the composition of the mycorrhizal fungal community in seven terrestrial orchid species inhabiting Song Mountain, Beijing, China, using Illumina MiSeq high-throughput sequencing. The mycorrhizal communities in the seven orchids were mainly composed of members of the Ceratobasidiaceae, Sebacinales, and Tulasnellaceae, while a number of ectomycorrhizal fungi belonging to the Russulaceae, Tricholomataceae, Thelephoraceae, and Cortinariaceae were occasionally observed. However, the dominant fungal associates and mycorrhizal community differed significantly among the orchid species as well as subhabitats. These findings confirm the previous observation that sympatric orchid species show different preferences for mycorrhizal fungi, which may drive niche partitioning and contribute to their cooccurrence. 展开更多
关键词 ORCHID MYCORRHIZA FUNGAL community terrestrial ORCHIDS FUNGAL diversity
XPS and FTIR studies of fungus-stained Daemonorops margaritae 预览
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作者 Wenhua Lyu Yuan Shi +1 位作者 Yaxian Zheng Xing’e Liu 《林业研究:英文版》 CAS CSCD 2019年第2期739-743,共5页
We explored the discoloration of rattan cane using X-ray photoelectron spectroscopy(XPS)and Fourier transform infrared spectroscopy(FTIR).XPS analysis showed that after the cane was stained by Lasiodiplodia theobromae... We explored the discoloration of rattan cane using X-ray photoelectron spectroscopy(XPS)and Fourier transform infrared spectroscopy(FTIR).XPS analysis showed that after the cane was stained by Lasiodiplodia theobromae,carbon and oxygen elements and the ratio of oxygen to carbon decreased.Considering atomic binding,C1 and C4 contents increased,while C2 and C3 contents decreased,and the ratio of O2 to O1 decreased sharply.The relative contents of lignin,cellulose and polysaccharides increased and new substances with low O2/O1 ratio occurred.FTIR analysis showed that the absorption peaks of O–H at 3346 cm^-1,aliphatic C–H at 2921,2853 and 1464 cm^-1,and C=O at 1723 cm^-1,were characteristic peaks of fungal melanin intensified,indicating that cane discoloration was primarily caused by fungal melanin.The absorption peaks characterizing cellulose and lignin like polysaccharides at 800 cm^-1,C–H at 1374 cm^-1,C–O at 1058 and 1038 cm^-1,phenolic hydroxyl at 1245 cm^-1,aromatic ether bonds at 1270 cm^-1,carbon skeleton at 1608 cm^-1 and benzene ring at 1500 cm^-1 were enhanced since the fungus mainly consumed the extractives in cane cell lumens and the main composition content increased relatively.Regardless of the discoloration caused by natural fungi or inoculated fungi,the discoloring feature and composition changes were identical except that the fungusinoculated cane had more melanin. 展开更多
关键词 RATTAN CANE FUNGAL stain FUNGAL MELANIN FTIR XPS
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Rapamycin liposome gutta inhibiting fungal keratitis of rats
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作者 Zhen-Hua Zhang Feng Teng +3 位作者 Qing-Xiu Sun Shu-Zhen Wang Chao Liu Gui-Qiu Zhao 《国际眼科杂志:英文版》 SCIE CAS 2019年第4期536-541,共6页
AIM: To study the therapeutic effect of rapamycin liposome eyedrops on fungal keratitis (FK) and its effect on the expression of monocyte chemotactic protein-1 (MCP-1). METHODS: This study adopted the thin film disper... AIM: To study the therapeutic effect of rapamycin liposome eyedrops on fungal keratitis (FK) and its effect on the expression of monocyte chemotactic protein-1 (MCP-1). METHODS: This study adopted the thin film dispersion method to prepare rapamycin liposomes eyedrops, as well as used the orthogonal design to analyze and study main influencing factors that affected the quality of liposomes. Totally 96 healthy Wistar rats were randomly divided into four groups: normal control group (A), FK blank control group (B), FK blank liposomes control group (C), and 30 FK rapamycin liposome treatment group (D). Groups B, C, and D were first prepared as FK animal models. The corneal response was recorded in details on day 1, 3, 5, 7, and 14 after modeling. Six rats were obtained and immunohistochemistry and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) were used to detect the expression of MCP-1 protein and mRNA, respectively. RESULTS: The severity of corneal lesions in the rapamycin treatment group was reduced, and the clinical score of the slit lamp examination was lower than that of Groups B and C (P<0.01). The expression of MCP-1 in rapamycin treatment group was significantly inhibited, comparing to that of groups B and C (P<0.01). CONCLUSION: Liposome is a good drug carrier for rapamycin. Rapamycin has a good therapeutic effect on FK. It can reduce FK fungal burden and significantly inhibit the expression of MCP-1 protein and mRNA. 展开更多
关键词 FUNGAL KERATITIS RAPAMYCIN LIPOSOME MONOCYTE chemotactic protein-1 RATS
Non-albicans Candida prosthetic joint infections: A systematic review of treatment 预览
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作者 Christos Koutserimpas Stylianos G Zervakis +4 位作者 Sofia Maraki Kalliopi Alpantaki Argyrios Ioannidis Diamantis P Kofteridis George Samonis 《世界临床病例杂志》 2019年第12期1430-1443,共14页
BACKGROUND Non-albicans Candida prosthetic joint infections (PJIs) are rare. Optimal treatment involves a two-stage revision surgery in combination with an antifungal agent. However, no clear guidelines have been deve... BACKGROUND Non-albicans Candida prosthetic joint infections (PJIs) are rare. Optimal treatment involves a two-stage revision surgery in combination with an antifungal agent. However, no clear guidelines have been developed regarding the agent or treatment duration. Hence, a broad range of antifungal and surgical treatments have been reported so far. AIM To clarify treatment of non-albicans Candida PJIs. METHODS A literature review of all existing non-albicans Candida PJIs cases through April 2018 was conducted. Information was extracted about demographics, comorbidities, responsible species, duration and type of antifungal treatment, type of surgical treatment, time between initial arthroplasty and symptom onset, time between symptom onset and definite diagnosis, outcome of the infection and follow-up. RESULTS A total of 83 cases, with a mean age of 66.3 years, were located. The causative yeast isolated in most cases was C. parapsilosis (45 cases;54.2%), followed by C. glabrata (18 cases;21.7%). The mean Charlson comorbidity index was 4.4 ± 1.5. The mean time from arthropalsty to symptom onset was 27.2 ± 43 mo, while the mean time from symptom onset to culture-confirmed diagnosis was 7.5 ± 12.5 mo. A two stage revision arthroplasty (TSRA), when compared to one stage revision arthroplasty, had a higher success rate (96% vs 73%, P = 0.023). Fluconazole was the preferred antifungal agent (59;71%), followed by amphotericin B (41;49.4%). CONCLUSION The combination of TSRA and administration of prolonged antifungal therapy after initial resection arthroplasty is suggested on the basis of limited data. 展开更多
关键词 Fungal PROSTHETIC JOINT INFECTION Knee ARTHROPLASTY INFECTION Hip ARTHROPLASTY INFECTION ANTIFUNGAL TREATMENT Non-albicans Candida PROSTHETIC JOINT infections
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Trait complementarity between fine roots of Stipa purpurea and their associated arbuscular mycorrhizal fungi along a precipitation gradient in Tibetan alpine steppe
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作者 ZHANG Jing WANG Ping +3 位作者 XUE Kai HAO Yan-bin WANG Yan-fen CUI Xiao-yong 《山地科学学报:英文版》 SCIE CSCD 2019年第3期542-547,共6页
Development of fine roots and formation of symbiosis with arbuscular mycorrhizal(AM) fungi represent two strategies for plants to acquire nutrient and water from soil. Here, we elucidated how fine root development and... Development of fine roots and formation of symbiosis with arbuscular mycorrhizal(AM) fungi represent two strategies for plants to acquire nutrient and water from soil. Here, we elucidated how fine root development and symbolized mycorrhizal fungi with Stipa purpurea responded to the precipitation change in Tibetan alpine steppe ecosystem across a precipitation gradient from 50 mm to 400 mm. As precipitation increased, the proportion of thinner fine roots(diameter < 0.4 mm) in total roots increased significantly;while the mycorrhizal colonization percentage, either associated with thinner or thicker roots, decreased. This phenomenon indicated that fine root development and symbolized mycorrhizal fungi are likely alternative, and plant preferred to develop fine root rather than build a symbiotic relationship with mycorrhizal fungi in more benign niches with higher precipitation. Also, root diameter was negatively correlated with specific root length(SRL), but positively correlated with AM fungal colonization percentage, indicating thicker-root species rely more on mycorrhizal fungi in alpine steppe. The complementarity between fine root and mycorrhizal fungi of S. purpurea is mediated by precipitation in Tibetan alpine steppe. 展开更多
关键词 ARBUSCULAR MYCORRHIZAL fungal colonization Root diameter Trait complementarity Precipitation STIPA PURPUREA ALPINE STEPPE
Ectomycorrhizal and Saprotrophic Fungal Communities Vary Across mm-Scale Soil Microsites Differing in Phosphatase Activity
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作者 Aaron GODIN Denise BROOKS +1 位作者 Sue J. GRAYSTON Melanie D. JONES 《土壤圈:英文版》 SCIE CAS CSCD 2019年第3期344-359,共16页
To understand nutrient cycling in soils, soil processes and microorganisms need be better characterized. To determine whether specific trophic groups of fungi are associated with soil enzyme activity, we used soil imp... To understand nutrient cycling in soils, soil processes and microorganisms need be better characterized. To determine whether specific trophic groups of fungi are associated with soil enzyme activity, we used soil imprinting to guide mm-scale sampling from microsites with high and low phosphatase activities in birch/Douglas-fir stands. Study 1 involved sampling one root window per site at 12 sites of different ages(stands);study 2 was conducted at one of the stem-exclusion stands, at which 5 root windows had been installed. Total fungal and ectomycorrhizal(EM) fungal terminal-restriction fragment length polymorphism(TRFLP)fingerprints differed between high-and low-phosphatase activity microsites at 8 of 12 root windows across 12 sites. Where differences were detected, fewer EM fungi were detected in high-than low-phosphatase activity microsites. Using 5 root windows at one site,next-generation sequencing detected similar fungal communities across microsites, but the ratio of saprotrophic to EM fungal reads was higher in high-phosphatase activity microsites in the two windows that had low EM fungal richness. In windows with differences in fungal communities, both studies indicated that EM fungi were less successful than saprotrophic fungi in colonizing fine-scale,organic matter-rich microsites. Fine-scale sampling linked with in situ detection of enzyme activity revealed relationships between soil fungal communities and phosphatase activity that could not be observed at the scales employed by conventional approaches, thereby contributing to the understanding of fine-scale phosphorus cycling in forest soils. 展开更多
关键词 ectomycorrhizae fine SCALE FUNGAL communities PHOSPHATASE root windows saprotrophic fungi SOIL IMPRINTING
A patatin-like protein synergistically regulated by jasmonate and ethylene signaling pathways plays a negative role in Nicotiana attenuata resistance to Alternaria alternata
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作者 Junbin Cheng Na Song Jinsong Wu 《植物多样性:英文版》 CAS CSCD 2019年第1期7-12,共6页
Although patatin was initially identified as a major storage protein in potato tubers, patatin-like proteins(PLPs) have been recently reported to be widely present in many plant species and shown to be involved in pla... Although patatin was initially identified as a major storage protein in potato tubers, patatin-like proteins(PLPs) have been recently reported to be widely present in many plant species and shown to be involved in plant-pathogen interactions. However, it is not clear whether PLPs are involved in Nicotiana attenuata resistance against the necrotrophic fungal pathogen, Alternaria alternata. In this study we identified a NaPLP gene, whose expression was highly elicited by A. alternata inoculation. Silencing NaPLP enhanced N. attenuata resistance to A. alternata, which was associated with higher induction levels of JA and ethylene biosynthetic genes, NaACS1, NaACO1 and NaLOX3. The induction of NaPLP expression by the fungus was abolished in JA-deficient plants and significantly reduced in ethylene-insensitive plants. In addition, NaPLP transcripts were highly induced by exogenous treatment with either methyl jasmonate(MeJA) or ethephon. Co-treatment with MeJA and ethephon led to a much higher induction level of NaPLP transcripts, and this synergistic induction was largely dependent on endogenous JA and ethylene signaling pathways. Thus, we conclude that the NaPLP gene is elicited by A. alternata via JA and ethylene signaling pathways in a synergistic way;however, unlike other JA-and ethylene-induced defense genes,NaPLP negatively affects plant resistance to the fungus likely by suppressing JA and ethylene biosynthetic gene expression. 展开更多
关键词 ALTERNARIA ETHYLENE JASMONATE Necrotrophic fungal pathogen NICOTIANA Plant RESISTANCE
2014—2017年解放军总医院海南分院真菌分布及耐药性分析
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作者 沈绍清 于帅 +1 位作者 陈怡君 蔡芸 《现代药物与临床》 CAS 2019年第1期252-256,共5页
目的了解2014—2017年解放军总医院海南分院真菌的分布和耐药特点,为促进抗真菌药的合理应用、有效减缓真菌耐药提供参考。方法提取解放军总医院海南分院2014年1月—2017年12月临床真菌分离和药敏相关数据,分析真菌的分布特征及其对常... 目的了解2014—2017年解放军总医院海南分院真菌的分布和耐药特点,为促进抗真菌药的合理应用、有效减缓真菌耐药提供参考。方法提取解放军总医院海南分院2014年1月—2017年12月临床真菌分离和药敏相关数据,分析真菌的分布特征及其对常用抗真菌药的耐药率和变化趋势。结果共纳入1048份阳性真菌样本,呼吸道标本占41.89%,60岁以上患者(61.45%)和重症医学科(25.48%)分布比例最高;共分离出1 329株真菌,白色念珠菌、念珠菌属、热带念珠菌分别占24.53%、15.80%、13.69%。各种念珠菌对两性霉素B和5-氟胞嘧啶的敏感率基本保持在90%以上;热带念珠菌对伊曲康唑耐药率最高,达10%~20%,对氟康唑和伏立康唑的耐药率亦高于两性霉素B和5-氟胞嘧啶;白色念珠菌对伊曲康唑和伏立康唑的耐药率呈逐年快速上升趋势。结论白色念珠菌和热带念珠菌感染应慎用唑类抗真菌药;两性霉素B和5-氟胞嘧啶是念珠菌感染的有效选择。 展开更多
关键词 真菌 热带念珠菌 抗真菌药 耐药性 感染
早产儿真菌性败血症临床分析(附16例报告) 预览
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作者 张佩 夏世文 刘勇 《实用医药杂志》 2019年第2期121-124,共4页
目的探讨早产儿真菌性败血症的临床特点,以提高该疾病的诊治水平。方法选择2013年1月—2014年9月湖北省妇幼保健院新生儿重症监护病房收治的早产儿真菌性败血症病例16例,对患者的一般状况、发病时间、首发症状、血小板、CRP、1,3-β-D... 目的探讨早产儿真菌性败血症的临床特点,以提高该疾病的诊治水平。方法选择2013年1月—2014年9月湖北省妇幼保健院新生儿重症监护病房收治的早产儿真菌性败血症病例16例,对患者的一般状况、发病时间、首发症状、血小板、CRP、1,3-β-D葡聚糖、血培养结果感染指标进行回顾性分析,比较治疗前与治疗中、治疗后的血小板、C反应蛋白及1,3-β-D葡聚糖的变化情况。结果新生儿真菌性败血症的病原菌以热带假丝酵母菌为主,发病日龄为生后5~36d,平均(14±5.5)d;喂养不耐受、频繁呼吸暂停、反应差、发热是新生儿真菌性败血症的常见首发症状;多数患儿出现血小板减少,C反应蛋白、1,3-β-D葡聚糖增高,抗真菌治疗7d后多数患者血小板可恢复正常,治疗2~3周后C反应蛋白、1,3-β-D葡聚糖可恢复正常,应用氟康唑、卡泊芬净治疗新生儿假丝酵母菌性败血症可取得满意疗效。结论早产儿真菌性败血症的临床表现缺乏特异性,对有高危因素的早产儿血小板减少、1,3-β-D葡聚糖增高可考虑早期真菌感染,氟康唑仍为首要治疗新生儿真菌性败血症一线药物,早期发现及时治疗是治疗成功的关键。 展开更多
关键词 败血症 真菌 早产儿 临床分析
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两性霉素B经支气管镜肺部局部注入的理论依据和操作流程 预览
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作者 冯靖 吴波 +1 位作者 张静 李彩丽 《天津医药》 CAS 北大核心 2019年第4期365-367,共3页
肺部真菌感染播散迅速,需药物及时干预。短期内即可形成组织坏死及肺局部结构性毁损,经有效抗真菌药物控制或经患者自身免疫局限后,可迅速形成纤维及肉芽组织包裹。两性霉素B是多烯类抗真菌药,抗真菌谱广且作用较强。经静脉应用时,常用... 肺部真菌感染播散迅速,需药物及时干预。短期内即可形成组织坏死及肺局部结构性毁损,经有效抗真菌药物控制或经患者自身免疫局限后,可迅速形成纤维及肉芽组织包裹。两性霉素B是多烯类抗真菌药,抗真菌谱广且作用较强。经静脉应用时,常用治疗量在肺部所达到的药物浓度对真菌仅具有抑菌作用,毒性大,不良反应多见;经气道黏膜吸收少而缓慢,刺激性不强。根据这些特点,两性霉素B经支气管镜肺部局部注入具有较大优势,值得临床推广。本文根据指南及临床实际应用经验,就两性霉素B经支气管镜肺部局部注入的理论依据和操作流程做详细说明,其中包括两性霉素B的药理机制、局部应用剂量和溶液浓度以及具体操作方法。 展开更多
关键词 两性霉素B 支气管镜检查 肺疾病 真菌性
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Antioxidant and Anti-inflammatory Capacity of Ferulic Acid Released from Wheat Bran by Solid-state Fermentation of Aspergillus niger 预览
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作者 YIN Zhi Na WU Wen Jia +10 位作者 SUN Chong Zhen LIU Hui Fan CHEN Wen Bo ZHAN Qi Ping LEI Zhuo Gui XIN Xuan MA Juan Juan YAO Kun MIN Tian ZHANG Meng Meng WU Hui 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第1期11-21,共11页
Objective A strain of Aspergillus niger(A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflamma... Objective A strain of Aspergillus niger(A. niger), capable of releasing bound phenolic acids from wheat bran, was isolated. This strain was identified by gene sequence identification. The antioxidant and anti-inflammatory capacity of ferulic acid released from wheat bran by this A. niger strain(FA-WB) were evaluated. Methods Molecular identification techniques based on PCR analysis of specific genomic sequences were conducted;antioxidant ability was examined using oxygen radical absorbance capacity(ORAC), cellular antioxidant activity(CAA) assays, and erythrocyte hemolysis assays. RAW264.7 cells were used as a model to detect anti-inflammatory activity. Results The filamentous fungal isolate was identified to be A. niger. ORAC and CAA assay showed that FA-WB had better antioxidant activity than that of the ferulic acid standard. The erythrocyte hemolysis assay results suggested that FA-WB could attenuate AAPH-induced oxidative stress through inhibition of reactive oxy gen species(ROS) generation. FA-WB could significantly restore the AAPH-induced increase in intracellular antioxidant enzyme activities to normal levels as well as inhibit the intracellular malondialdehyde formation. TNF-?, IL-6, and NO levels indicated that FA-WB can inhibit the inflammation induced by lipopolysaccharide(LPS). Conclusion Ferulic acid released from wheat bran by a new strain of A. niger had good anti-inflammatory activity and better antioxidant ability than standard ferulic acid. 展开更多
关键词 FUNGAL identification Fermentation Ferulic acid ANTIOXIDANT ACTIVITY Oxidative HEMOLYSIS ANTI-INFLAMMATORY activity.
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Expression and role of calcitonin gene-related peptide in mouse Aspergillus fumigatus keratitis
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作者 Min Yin Cui Li +6 位作者 Xu-Dong Peng Gui-Qiu Zhao Yuan Wu Heng-Rui Zheng Qian Wang Qiang Xu Nan Jiang 《国际眼科杂志:英文版》 SCIE CAS 2019年第5期697-704,共8页
AIM: To investigate the expression and role of calcitonin gene-related peptide(CGRP) in the mouse models induced by Aspergillus fumigatus(A. fumigatus). METHODS: C57 BL/6 mice were randomized into a control group and ... AIM: To investigate the expression and role of calcitonin gene-related peptide(CGRP) in the mouse models induced by Aspergillus fumigatus(A. fumigatus). METHODS: C57 BL/6 mice were randomized into a control group and A. fumigatus keratitis group. The cornea photography was assessed under the slit lamp and the clinical score was recorded after infection. Western blot, real-time polymerase chain reaction(PCR) and immunohistofluorescence analysis were applied to detect CGRP expression in cornea of both groups. In vitro, tests were conducted with C57 BL/6 mice macrophages to investigate CGRP expression after interaction with A. fumigatus. Cytokines expression induced by exogenous CGRP and the antagonist CGRP8-37 in A. fumigatus-exposed macrophages was evaluated by real-time PCR and ELISA.RESULTS: The cornea expression of CGRP was significantly elevated in C57 BL/6 mice corneas and macrophages after A. fumigatus infection. After treatment with exogenous CGRP, the levels of interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α) and IL-6 were reduced, and IL-10 level was increased in the A. fumigatus stimulatedmacrophages. However, IL-1β, TNF-α and IL-6 levels were upregulated after pretreatment of CGRP8-37. But the m RNA levels of MIP-2, TGF-β and IL-10 were not changed. CONCLUSION: This study provides evidence that A. fumigatus increased CGRP expression. CGRP may play a protective role against inflammation in A. fumigatus keratitis. 展开更多
关键词 CALCITONIN gene-related PEPTIDE FUNGAL KERATITIS MACROPHAGES INFLAMMATION mice
Successful treatment of invasive fungal rhinosinusitis caused by Cunninghamella:A case report and review of the literature 预览
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作者 Yong-Cai Liu Min-Li Zhou +3 位作者 Ke-Jia Cheng Shui-Hong Zhou Xue Wen Cheng-Dong Chang 《世界临床病例杂志》 2019年第2期228-235,共8页
BACKGROUND Invasive fungal rhinosinusitis(IFR)caused by Cunninghamella is very rare but has an extremely high fatality rate.There have been only seven cases of IFR caused by Cunninghamella reported in English and,of t... BACKGROUND Invasive fungal rhinosinusitis(IFR)caused by Cunninghamella is very rare but has an extremely high fatality rate.There have been only seven cases of IFR caused by Cunninghamella reported in English and,of these,only three patients survived.In this article,we present another case of IFR caused by Cunninghamella,in which the patient was initially treated successfully but then deteriorated due to a relapse of leukemia 2 mo later.CASE SUMMARY A 50-year-old woman presented with a 2-mo history of right ocular proptosis,blurred vision,rhinorrhea and nasal obstruction.Nasal endoscopic examination showed that the middle turbinate had become necrotic and fragile.Endoscopic sinus surgery and enucleation of the right orbital contents were performed successively.Additionally,the patient was treated with amphotericin B both systematically and topically.Secretion cultivation of the right eye canthus showed infection with Cunninghamella,while postoperative pathology also revealed fungal infection.The patient’s condition gradually stabilized after surgery.However,the patient underwent chemotherapy again due to a relapse of leukemia 2 mo later.Unfortunately,her leukocyte count decreased dramatically,leading to a fatal lung infection and hemoptysis.CONCLUSION Aggressive surgical debridements,followed by antifungal drug treatment both systematically and topically,are the most important fundamental treatments for IFR. 展开更多
关键词 CUNNINGHAMELLA INVASIVE FUNGAL RHINOSINUSITIS Acute MYELOID LEUKEMIA Treatment Prognosis Case report
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Disparate expression of autophagy in corneas of C57BL/6 mice and BALB/c mice after Aspergillus fumigatus infection
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作者 Chen-Yu Li Cui Li +7 位作者 Hui Li Gui-Qiu Zhao Jing Lin Qian Wang Xu-Dong Peng Qiang Xu Guo-Qiang Zhu Jia-Qian Jiang 《国际眼科杂志:英文版》 SCIE CAS 2019年第5期705-710,共6页
AIM: To determine the disparate expression of autophagy in the Aspergillus fumigatus(A. fumigatus) keratitis between susceptible C57 BL/6 mice and resistant BALB/c mice.METHODS: C57 BL/6 and BALB/c mice were used to e... AIM: To determine the disparate expression of autophagy in the Aspergillus fumigatus(A. fumigatus) keratitis between susceptible C57 BL/6 mice and resistant BALB/c mice.METHODS: C57 BL/6 and BALB/c mice were used to establish fungal keratitis models. Disease severity and inflammatory response were observed by slit lamp microscopy in A. fumigatus-infected corneas of C57 BL/6 and BALB/c mice at 1, 3 and 5 d. Hematoxylin-eosin(H&E) staining was used to detect pathological changes of corneas. The expression of autophagy-related proteins Beclin-1, LC3, SQSTM1/p62, and LAMP-1 was assessed by Western blot in C57 BL/6 and BALB/c mice at 1, 3 and 5 d post infection(p.i.). Immunofluorescent staining was used to test the expression of LC3 in corneas after A. fumigatus infection.RESULTS: Keratitis severity was higher in C57 BL/6 mice versus BALB/c mice at 1, 3 and 5 d p.i. H&E staining showed that the number of inflammatory cells was larger and the severity of ulcer was higher in C57 BL/6 mice than in BALB/c mice after stimulation with A. fumigatus. Higher expression of LAMP-1, Beclin-1, and LC3 was shown in C57 BL/6 mice corneas than in BALB/c mice corneas at 1, 3 and 5 d p.i., while the expression of p62 was lower in C57 BL/6 mice. The fluorescence of LC3 was significantly increased in corneas of C57 BL/6 mice compared with BALB/c mice after A. fumigatus infection.CONCLUSION: The expression of autophagy is higher in corneas of C57 BL/6 mice than in BALB/c mice afterA. fumigatus infection. Autophagy may be positively correlated with keratitis severity and pathological changes. 展开更多
关键词 FUNGAL KERATITIS ASPERGILLUS FUMIGATUS AUTOPHAGY
Expression of macrophage migration inhibitory factor in Aspergillus fumigatus keratitis
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作者 Qiang Xu Li-Ting Hu +4 位作者 Qian Wang Jing Lin Nan Jiang Cui Li Gui-Qiu Zhao 《国际眼科杂志:英文版》 SCIE CAS 2019年第5期711-716,共6页
AIM: To investigate the expression of macrophage migration inhibitory factor(MIF) and detect its role in the innate immune response of fungal keratitis(FK). METHODS: We collected the paraffin-embedded cornea tissues f... AIM: To investigate the expression of macrophage migration inhibitory factor(MIF) and detect its role in the innate immune response of fungal keratitis(FK). METHODS: We collected the paraffin-embedded cornea tissues from 10 FK and 6 ocular trauma patients to explore the MIF expression by immunohistochemistry. Then we cultured telomease-immortalized human corneal epithelial cells(THCEs), stimulated by the hyphae suspension of Aspergillus fumigatus(A. fumigatus) to detect the change of MIF with or without the pretreatment of MIF inhibitor [4-Iodo-6-phenylpyrimidine(4-IPP)] by real-time polymerase chain reaction(PCR). The protein level of MIF was also tested by immunohistochemistry, and the level of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) mRNA were compared between normal, hyphae stimulated and 4-IPP pretreated groups by real-time PCR to study the influence of MIF on the expression of TNF-α and IL-6. Corneal severity of rats’ FK models was documented by clinical scores, and real-time PCR. Western blot and immunohistochemistry were used to test the expression of MIF, TNF-α and IL-6 in rats’ corneas.RESULTS: In the corneas of FK patients, there was much stronger expression of MIF than that in the normal group showed by immunohistochemistry. In cultured THCEs stimulated by A. fumigatus, the expression of MIF became stronger in both immunohistochemistry and PCR at 16, 24, 32 and 48 h post infection(p.i.;P<0.01, P<0.01, P<0.01, P<0.05). After pretreated with 4-IPP, the expression of MIF reduced at 4, 8, 16 h p.i.(P<0.05, P<0.05, P<0.05) and the downstream TNF-α and IL-6 decreased obviously(P<0.05, P<0.01). In rats with A. fumigatus keratitis, the relative mRNA and protein level of MIF increased than thosein the normal group by PCR(at 1 d: P<0.01, 3 d: P<0.01, 5 d: P<0.01), Western blot and immunohistochemistry. After blocked MIF with 4-IPP, the clinical outcomes of rat keratitis showed markedly reduced inflammatory response(P<0.01), with TNF-α and IL-6 decreased in accordance with those in THCEs 展开更多
关键词 macrophage migration INHIBITORY factor FUNGAL KERATITIS INNATE immune A. FUMIGATUS CORNEAL epithelial cells rats
中药抗真菌作用的研究进展
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作者 冯佳佳 陶元勇 《实用皮肤病学杂志》 2019年第1期34-37,共4页
中药作为抗真菌药物,具有价格低廉、资源丰富、不良反应小、不易耐药等优点。该文通过对单味中药、复方中药、中西药协同抗真菌作用及中药抗真菌作用的机制及近年来中药抗真菌的研究现状进行综述,为临床抗真菌治疗提供理论依据。
关键词 真菌 中药 研究进展
Changes in fungal community and diversity in strawberry rhizosphere soil after 12 years in the greenhouse 预览
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作者 LI Wei-hua LIU Qi-zhi 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第3期677-687,共11页
Soil fungi play a very important role in the soil ecological environment. In agricultural production, long-term monoculture and continuous cropping lead to changes in fungal community diversity. However, the effects o... Soil fungi play a very important role in the soil ecological environment. In agricultural production, long-term monoculture and continuous cropping lead to changes in fungal community diversity. However, the effects of long-term monoculture and continuous cropping on strawberry plant health and fungal community diversity have not been elucidated. In this study, using high-throughput sequencing(HTS), we compared the fungal community and diversity of strawberry rhizosphere soil after various durations of continuous cropping(0, 2, 4, 6, 8, 10 and 12 years). The results showed that soil fungal diversity increased with consecutive cropping years. Specifically, the soil-borne disease pathogens Fusarium and Guehomyces were significantly increased after strawberry continuous cropping, and the abundance of nematicidal(Arthrobotrys) fungi decreased from the fourth year of continuous cropping. The results of correlation analysis suggest that these three genera might be key fungi that contribute to the changes in soil properties that occur during continuous cropping. In addition, physicochemical property analysis showed that the soil nutrient content began to decline after the fourth year of continuous cropping. Spearman’s correlation analysis showed that soil pH, available potassium(AK) and ammonium nitrogen(NH4+-N) were the most important edaphic factors leading to contrasting beneficial and pathogenic associations across consecutive strawberry cropping systems. 展开更多
关键词 FUNGAL community soil-borne disease replanted STRAWBERRY RHIZOSPHERE SOIL agricultural SOIL ECOLOGY
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Conjunctival microbiome changes associated with fungal keratitis:metagenomic analysis
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作者 Cheng Ge Chao Wei +2 位作者 Bao-Xia Yang Jun Cheng Yu-Sen Huang 《国际眼科杂志:英文版》 SCIE CAS 2019年第2期194-200,共7页
AIM:To investigate the ocular surface microbiome profile of patients with fungal keratitis(FK) through bacterial 16 S r DNA sequencing. METHODS:The swab samples were collected from 8 patients with FK(Group 1 from the ... AIM:To investigate the ocular surface microbiome profile of patients with fungal keratitis(FK) through bacterial 16 S r DNA sequencing. METHODS:The swab samples were collected from 8 patients with FK(Group 1 from the corneal ulcer, Group 2 from the conjunctival sac of the infected eyes, and Group 3 from the conjunctival sac of the fellow eyes) and 10 healthy eyes(Group 4 from the conjunctival sac). Bacterial 16 S rDNA V4-V5 region sequencing was performed to characterize the bacterial communities on the ocular surfaces of the patients with FK. RESULTS:Our metagenomic data showed that 97% of the sequence reads were categorized into 245 distinct bacterial genera, with 67.75±7.79 genera detected in Group 1, 73.80±13.44 in Group 2, 74.57±14.14 in Group 3, and 89.60±27.49 in Group 4. Compared with the healthy eyes(Group 4), both infected(Groups 1 and 2) and fellow eyes(Group 3) of the patients with FK showed reduced bacterial diversity and altered ocular surface microbiota compositions, with lower abundance of Corynebacterium and Staphylo coccus and higher abundances of Pseudomonas, Achromobacter, Caulobacter and Psychrobacter. CONCLUSION:Our report depicts the altered ocular surface bacterial community structures both in the affected and fellow eyes of patients with FK. These changes may contribute to the pathogenesis of FK or the increased risk for FK. 展开更多
关键词 CONJUNCTIVA MICROBIOTA bacteria METAGENOMICS 16S rDNA fungal KERATITIS
Clinical risk score for invasive fungal diseases in patients with hematological malignancies undergoing chemotherapy:China Assessment of Antifungal Therapy in Hematological Diseases (CAESAR) study
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作者 Ling Wang Ying Wang +16 位作者 Jiong Hu Yuqian Sun He Huang Jing Chen Jianyong Li Jun Ma Juan Li Yingmin Liang Jianmin Wang Yan Li Kang Yu Jianda Hu Jie Jin Chun Wang Depei Wu Yang Xiao Xiaojun Huang 《医学前沿:英文版》 CAS CSCD 2019年第3期365-377,共13页
Invasive fungal disease (IFD) is a major infectious complication in patients with hematological malignancies.In this study,we examined 4889 courses of chemotherapy in patients with hematological diseases to establish ... Invasive fungal disease (IFD) is a major infectious complication in patients with hematological malignancies.In this study,we examined 4889 courses of chemotherapy in patients with hematological diseases to establish a training dataset (n=3500) by simple random sampling to develop a weighted risk score for proven or probable IFD through multivariate regression,which included the following variables: male patients,induction chemotherapy for newly diagnosed or relapsed disease,neutropenia,neutropenia longer than 10 days,hypoalbuminemia,central-venous catheter,and history of IFD.The patients were classified into three groups,which had low (0-10,~1.2%),intermediate (11-15,6.4%),and high risk (> 15,17.5%) of IFD.In the validation set (n=1389),the IFD incidences of the groups were ~1.4%,5.0%,and 21.4%.In addition,we demonstrated that antifungal prophylaxis offered no benefits in low-risk patients,whereas benefits were documented in intermediate (2.1% vs.6.6%,P=0.007) and high-risk patients (8.4% vs.23.3%,P=0.007).To make the risk score applicable for clinical settings,a pre-chemo risk score that deleted all unpredictable factors before chemotherapy was established,and it confirmed that anti-fungal prophylaxis was beneficial in patients with intermediate and high risk of IFD.In conclusion,an objective,weighted risk score for IFD was developed,and it may be useful in guiding antifungal prophylaxis. 展开更多
关键词 invasive FUNGAL diseases HEMATOLOGICAL MALIGNANCIES CHEMOTHERAPY risk score PROPHYLAXIS
呼吸科病房老年病人肺部感染常见病原菌及其耐药性分析 预览
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作者 褚月娇 周楚铭 +3 位作者 韩莹莹 刘丹 万小旭 王佳贺 《安徽医药》 CAS 2019年第1期63-66,共4页
目的对老年病人(年龄≥65岁)肺部感染的常见病原菌及其耐药性进行分析,为临床预防和治疗老年肺部感染提供依据。方法回顾性收集中国医科大学附属盛京医院2013年1月至2015年12月老年病人感染的常见病原菌并对其耐药性进行分析。结果共分... 目的对老年病人(年龄≥65岁)肺部感染的常见病原菌及其耐药性进行分析,为临床预防和治疗老年肺部感染提供依据。方法回顾性收集中国医科大学附属盛京医院2013年1月至2015年12月老年病人感染的常见病原菌并对其耐药性进行分析。结果共分离出318株病原菌,其中革兰阴性杆菌295例,占92.77%。其中鲍曼不动杆菌最多,占43.71%;其次是铜绿假单胞菌和肺炎克雷伯杆菌,分别占16.98%和12.89%。共分离出革兰阳性球菌20例,占6.29%。真菌3例,占0.94%。不同病原菌耐药程度不同,其中鲍曼不动杆菌对青霉素类、头孢类抗生素耐药率达90%~100%,对氨基糖苷类抗生素耐药率亦达38%~90%,仅替加环素耐药率为10.31%。铜绿假单胞菌耐药亦较严重。肺炎克雷伯杆菌耐药率相对乐观,临床可选药物较多。金黄色葡萄球菌对青霉素、头孢类抗生素几乎均耐药,对万古霉素、利奈唑胺、喹努普汀/达福普汀及替加环素最为敏感。结论老年肺部感染以革兰阴性杆菌为主,临床应根据药敏实验结果合理应用抗生素来更好地控制疾病和防止产生耐药菌。 展开更多
关键词 呼吸道感染 抗药性 细菌 抗药性 真菌 革兰阳性菌感染 革兰阴性菌感染 病房 老年人
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