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Ethanol extract from Gynostemma pentaphyllum ameliorates dopaminergic neuronal cell death in transgenic mice expressing mutant A53T human alpha-synuclein 预览
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作者 Hyun Jin Park Ting Ting Zhao +4 位作者 Seung Hwan Kim Chong Kil Lee Bang Yeon Hwang Kyung Eun Lee Myung Koo Lee 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期361-368,共8页
Gynostemma(G.) pentaphyllum(Cucurbitaceae) contains various bioactive gypenosides. Ethanol extract from G. pentaphyllum(GP-EX) has been shown to have ameliorative effects on the death of dopaminergic neurons in animal... Gynostemma(G.) pentaphyllum(Cucurbitaceae) contains various bioactive gypenosides. Ethanol extract from G. pentaphyllum(GP-EX) has been shown to have ameliorative effects on the death of dopaminergic neurons in animal models of Parkinson’s disease(PD) induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-and 6-hydroxydopamine. PD patients exhibit multiple symptoms, so PD-related research should combine neurotoxin models with genetic models. In the present study, we investigated the ameliorative effects of GP-EX, including gypenosides, on the cell death of dopaminergic neurons in the midbrain of A53 T α-synuclein transgenic mouse models of PD(A53 T). Both GP-EX and gypenosides at 50 mg/kg per day were orally administered to the A53 T mice for 20 weeks.α-Synuclein-immunopositive cells and α-synuclein phosphorylation were increased in the midbrain of A53 T mice, which was reduced following treatment with GP-EX. Treatment with GP-EX modulated the reduced phosphorylation of tyrosine hydroxylase, extracellular signal-regulated kinase(ERK1/2), Bcl-2-associated death promoter(Bad) at Ser112, and c-Jun N-terminal kinase(JNK1/2) due to α-synuclein overexpression. In the A53 T group, GP-EX treatment prolonged the latency of the step-through passive avoidance test and shortened the transfer latency of the elevated plus maze test. Gypenosides treatment exhibited the effects and efficacy similar to those of GP-EX. Taken together, GP-EX, including gypenosides, has ameliorative effects on dopaminergic neuronal cell death due to the overexpression of α-synuclein by modulating ERK1/2, Bad at Ser112, and JNK1/2 signaling in the midbrain of A53 T mouse model of PD. Further studies are needed to investigate GP-EX as a treatment for neurodegenerative synucleinopathies, including PD. This study was approved by the Animal Ethics Committee of Chungbuk National University(approval No. CBNUA-956-16-01) on September 21, 2016. 展开更多
关键词 A53T α-synuclein genetic mice ERK1/2 GYNOSTEMMA pentaphyllum GYPENOSIDES Parkinson’s disease retention transfer LATENCY time
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Can mouse models mimic sporadic Alzheimer’s disease? 预览
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作者 Bettina MFoidl Christian Humpel 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期401-406,共6页
Alzheimer’s disease(AD)is a progressive neurodegenerative disorder and the most common form of dementia worldwide.As age is the main risk factor,>97%of all AD cases are of sporadic origin,potentiated by various ri... Alzheimer’s disease(AD)is a progressive neurodegenerative disorder and the most common form of dementia worldwide.As age is the main risk factor,>97%of all AD cases are of sporadic origin,potentiated by various risk factors associated with life style and starting at an age>60 years.Only<3%of AD cases are of genetic origin caused by mutations in the amyloid precursor protein or Presenilins 1 or 2,and symptoms already start at an age<30 years.In order to study progression of AD,as well as therapeutic strategies,mouse models are state-of-the-art.So far many transgenic mouse models have been developed and used,with mutations in the APP or presenilin or combinations(3×Tg,5×Tg).However,such transgenic mouse models more likely mimic the genetic form of AD and no information can be given how sporadic forms develop.Several risk genes,such as Apolipoprotein E4 and TREM-2 enhance the risk of sporadic AD,but also many risk factors associated with life style(e.g.,diabetes,hypercholesterolemia,stress)may play a role.In this review we discuss the current situation regarding AD mouse models,and the problems to develop a sporadic mouse model of AD. 展开更多
关键词 Alzheimer’s disease BETA-AMYLOID cerebral AMYLOID ANGIOPATHY cognitive impairment SPORADIC and genetic mouse models tau vascular risk factors
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Genetic testing in neurology exploiting next generation sequencing: state of art 预览
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作者 Chiara Di Resta Maurizio Ferrari 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期265-266,共2页
Next generations sequencing (NGS) is definitely one of the most revolutionary technology of the last years in genetic and medical field (Kricka and Di Resta, 2013). It brought important changes in genetic testing of i... Next generations sequencing (NGS) is definitely one of the most revolutionary technology of the last years in genetic and medical field (Kricka and Di Resta, 2013). It brought important changes in genetic testing of inherited human disorders, in particular in neurological Mendelian forms, such as inherited neuropathies, ataxias or monogenic form of epilepsy, where “diagnostic odyssey” is quite common. 展开更多
关键词 GENETIC testing NGS DIAGNOSTIC ODYSSEY
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Evaluation of Genetic Diversity and Genetic Relationship of Large Fruited Tomato Core Germplasms Based on Genotypic Value 预览
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作者 Liu Ziji Liu Weixia +1 位作者 Niu Yu Yang Yan 《Plant Diseases and Pests(植物病虫害研究:英文版)》 CAS 2019年第3期1-4,共4页
[Objective] The paper aimed to analyze genetic diversity and genetic relationship of large fruited tomato core germplasms based on genotypic value.[Method] Taking 55 large fruited tomato core germplasms as tested mate... [Objective] The paper aimed to analyze genetic diversity and genetic relationship of large fruited tomato core germplasms based on genotypic value.[Method] Taking 55 large fruited tomato core germplasms as tested materials, the genetic diversity of seven agronomic traits was analyzed, and the correlation between traits and genetic relationship between germplasms were analyzed based on genotypic effect value.[Result] The diversity indexes of seven agronomic traits were 3.98, 3.97, 3.99, 3.99, 3.97, 3.98 and 3.87, respectively, indicating there were rich genetic diversities in large fruited tomato core germplasms. Correlation analysis of traits was conducted based on genotypic effect value. The results showed that there was extremely significant positive correlation between leaf length and leaf width, and the correlation coefficient was 0.66. There was extremely significant positive correlation between fruit length and fruit width, with a correlation coefficient of 0.49. There was extremely significant positive correlation between weight per fruit and fruit width, with a correlation coefficient of 0.46. The genetic distance between large fruited tomato core germplasms was calculated based on the phenotypic value of traits. When the distance was 5, 55 large fruited tomato core germplasms were divided into three groups. The genetic distance between large fruited core germplasms tomato was calculated based on genotypic predictive value of traits. When the distance was 10, 55 large fruited tomato core germplasms were divided into six groups. Comparing the results of two clustering analysis, it was found that the clustering analysis based on genotypic value could better explain the genetic relationship between germplasms.[Conclusion] The results could provide some basis for understanding the population variation of large fruited tomato and genetic improvement of tomato cultivars. 展开更多
关键词 Large fruited TOMATO PHENOTYPIC VALUE GENOTYPIC VALUE GENETIC diversity Correlation GENETIC relationship
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Current status of the genetic susceptibility in attenuated adenomatous polyposis 预览
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作者 Víctor Lorca Pilar Garre 《世界胃肠肿瘤学杂志:英文版(电子版)》 CAS 2019年第12期1101-1114,共14页
Adenomatous polyposis(AP)is classified according to cumulative adenoma number in classical AP(CAP)and attenuated AP(AAP).Genetic susceptibility is the major risk factor in CAP due to mutations in the known high predis... Adenomatous polyposis(AP)is classified according to cumulative adenoma number in classical AP(CAP)and attenuated AP(AAP).Genetic susceptibility is the major risk factor in CAP due to mutations in the known high predisposition genes APC and MUTYH.However,the contribution of genetic susceptibility to AAP is lower and less understood.New predisposition genes have been recently proposed,and some of them have been validated,but their scarcity hinders accurate risk estimations and prevalence calculations.AAP is a heterogeneous condition in terms of severity,clinical features and heritability.Therefore,clinicians do not have strong discriminating criteria for the recommendation of the genetic study of known predisposition genes,and the detection rate is low.Elucidation and knowledge of new AAP high predisposition genes are of great importance to offer accurate genetic counseling to the patient and family members.This review aims to update the genetic knowledge of AAP,and to expound the difficulties involved in the genetic analysis of a highly heterogeneous condition such as AAP. 展开更多
关键词 Attenuated adenomatous polyposis Genetic susceptibility High predisposition gene Genetic heterogeneity Colorectal cancer
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Comparison,Selection and Progeny Test of Natural Populations of White Birch(Betula platyphylla Suk.)in Northern China 预览
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作者 Shibo LAN 《农业生物技术:英文版》 CAS 2019年第4期113-118,共6页
[Objectives]This study was conducted to rationally and effectively use natural genetic resources of white birch(Betula platyphylla Suk.),and improve the scientific and technological content of improved varieties.[Meth... [Objectives]This study was conducted to rationally and effectively use natural genetic resources of white birch(Betula platyphylla Suk.),and improve the scientific and technological content of improved varieties.[Methods]With the progeny of the white birch populations naturally distributed in northern China as objects,based on genetic effect analysis and comprehensive evaluation,the genetic variation,heritability and genetic gain of the growth characters in the progeny forest were systematically analyzed,by which populations or individuals with good growth vigor and strong stress resistance and adaptability were finally selected.[Results]There were extremely significant differences in the growth characters between the natural populations of white birch in northern China.The variation was the smallest in tree height,followed by crown width,and largest in DBH,and the average coefficients of variation were 20.56%,32.25%and 33.00%,respectively.The differences in characters between progeny of the same population were also extremely significant.The growth characters of white birch were more controlled by genes,and the genetic gains were larger.The heritability of tree height,DBH and crown width was 0.971,0.816 and 0.576,respectively,and the genetic gains were 15.97%,21.54%and 13.87%,respectively.[Conclusions]With the growth characters as the main evaluation indexes,taking various influencing factors into account,the Shanxi[SX]population,Hebei[HB]population and Liaoning[LN]population were selected to be excellent populations,and LN305,SX516 and HB403 were excellent individuals,which are advised to be widely used in multi-generation genetic improvement breeding. 展开更多
关键词 BETULA platyphylla Suk. Natural population COMPARISON and SELECTION PROGENY test GENETIC effect GENETIC gain
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Gene redundancy and gene compensation:An updated view
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作者 Jinrong Peng 《遗传学报:英文版》 SCIE CAS CSCD 2019年第7期329-333,共5页
Gene knockdown approaches using antisense oligo nucleotides or analogs such as siRNAs and morpholinos have been widely adopted to study gene functions although the off-target issue has been always a concern in these s... Gene knockdown approaches using antisense oligo nucleotides or analogs such as siRNAs and morpholinos have been widely adopted to study gene functions although the off-target issue has been always a concern in these studies.On the other hand,classic genetic analysis relies on the availability of loss-offunction or gain-of-function mutants.The fast development of genome editing technologies such as TALEN and CRISPR/Cas9 has greatly facilitated the generation of null mutants for the functional studies of target genes in a variety of organisms such as zebrafish.Surprisingly,an unexpected discrepancy was observed between morphant phenotype and mutant phenotype for many genes in zebrafish,i.e.,while the morphant often displays an obvious phenotype,the corresponding null mutant appears relatively normal or only exhibits a mild phenotype due to gene compensation.Two recent reports have partially answered this intriguing question by showing that a pre-mature termination codon and homologous sequence are required to elicit the gene compensation and the histone modifying complex COMPASS is involved in activating the expression of the compensatory genes.Here,I summarize these exciting new progress and try to redefine the concept of genetic compensation and gene compensation. 展开更多
关键词 GENE REDUNDANCY GENE COMPENSATION GENETIC COMPENSATION Non-sense-mediated RNA decay(NMD) COMPASS complex GENETIC model system Zebrafish Capn3 Upf3a
Shallow Genetic Structure of Pholis fangi in Bohai Sea andYellow Sea Inferred from mtDNA Control Region 预览
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作者 GAO Tianxiang LI Lin +4 位作者 FANG Rundong LIU Guanghui WANG Lei XU Hanxiang SONG Na 《中国海洋大学学报:英文版》 SCIE CAS CSCD 2019年第4期947-952,共6页
Pholis fangi is an ecologically important fish species inhabiting Chinese coastal waters of Yellow Sea and Bohai Sea. To investigate the genetic diversity and population genetic structure of P. fangi, a fragment of 48... Pholis fangi is an ecologically important fish species inhabiting Chinese coastal waters of Yellow Sea and Bohai Sea. To investigate the genetic diversity and population genetic structure of P. fangi, a fragment of 487 bp in the first hypervariable region (HVR-1) of the mitochondrial DNA (mtDNA) control region was sequenced for 181 individuals collected from Bohai Sea and Yel- low Sea. A total of 18 polymorphic sites were detected, which defined 25 haplotypes. A moderate level of haplotype diversity (h = 0.7052) and a low level of nucleotide diversity (π= 0.0028) were detected. Both the phylogenetic tree and the haplotype network showed no significant genealogical structure difference among sampling locations. Pairwise FST comparison and hierarchical mo- lecular variance analysis (AMOVA) revealed that no significant genetic structure difference existed throughout the investigated re- gions, suggesting a high gene exchange among different populations. The results of neutrality test and mismatch distribution analysis indicated that a late Pleistocene population expansion (38000 127000 years ago) happened. Seasonal schooling migration may con- tribute to the genetically homogeneous population structure of the species. 展开更多
关键词 Pholis fangi control region of mtDNA GENETIC diversity GENETIC structure DEMOGRAPHIC history
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Identification of patients with pancreatic adenocarcinoma due to inheritable mutation:Challenges of daily clinical practice 预览
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作者 Alexander JP Fulton Angela Lamarca +8 位作者 Christina Nuttall Lynne McCallum Rille Pihlak Derek O’Reilly Fiona Lalloo Mairéad G McNamara Richard A Hubner Tara Clancy Juan W Valle 《世界胃肠肿瘤学杂志:英文版(电子版)》 CAS 2019年第2期102-116,共15页
BACKGROUND Identification of germ-line mutations in pancreatic ductal adenocarcinoma(PDAC)could impact on patient/family.AIM To assess the referral pathways for genetic consultations in PDAC.METHODS Electronic records... BACKGROUND Identification of germ-line mutations in pancreatic ductal adenocarcinoma(PDAC)could impact on patient/family.AIM To assess the referral pathways for genetic consultations in PDAC.METHODS Electronic records of PDAC patients were reviewed retrospectively.Patients eligible for genetic consultation referral were identified following the European Registry of Hereditary Pancreatitis and Familial Pancreatic Cancer(EUROPAC)criteria.RESULTS Four-hundred patients were eligible.Of 113 patients(28.3%)meeting EUROPAC criteria,8.8%were referred for genetic opinion.Germ-line mutations were identified in 0.75%of the whole population.CONCLUSION Earlier referrals and increased awareness may be able to overcome the low rate of successful genetic appointments. 展开更多
关键词 GENETIC COUNSELLING Pancreatic ADENOCARCINOMA GENETIC CONSULTATION BRCA GERMLINE
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Genome-wide developed microsatellite markers for the melon thrips Thrips palmi Karny(Thysanoptera:Thripidae)
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作者 Yongfu Gao Yajun Gong +4 位作者 Ling Ma Lijun Cao Jincui Chen Min Chen Shujun Wei 《动物分类学报:英文版》 CSCD 2019年第2期100-110,共11页
The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtain... The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtained randomly sequenced regions from the genome using next-generation sequencing and assembled 356 Mb genomic sequences. In total, 155,789 microsatellites were identified from the genomic sequences, of which, 64.02% were loci of dinucleotide repeat. Sixty primer pairs were initially validated in seven individuals. Thirty-five polymorphic markers were retained according to the amplification efficiency and polymorphism.These 35 microsatellite loci were then assessed in 96 individuals in four geographical populations collected from China. The allele numbers ranged from 2 to 19 with an average value of 6.9 per locus, while the polymorphism information content ranged from 0.2219 to 0.8490.The observed and expected heterozygosity varied from 0.5252 to 0.5367 and 0.5934 to 0.6148,respectively. Genetic structure was found between populations from southern and northern China. These novel microsatellite markers developed in our study provide abundant molecular markers for the genetic study of T. palmi. 展开更多
关键词 MICROSATELLITE GENETIC marker genome population GENETIC structure
基于遗传算法的带有调纵倾尾板及拦截器的滑行艇水动力性能评估及优化 预览
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作者 Abdollah Sakaki Hassan Ghassemi Shayan Keyvani 《船舶与海洋工程学报:英文版》 CSCD 2019年第2期131-141,共11页
Nowadays,several stern devices are attracting a great deal of attention.The control surface is an effective apparatus for improving the hydrodynamic performance of planing hulls and is considered an important element ... Nowadays,several stern devices are attracting a great deal of attention.The control surface is an effective apparatus for improving the hydrodynamic performance of planing hulls and is considered an important element in the design of planing hulls.Control surfaces produce forces and a pitching moment due to the pressure distribution that they cause,which can be used to change the running state of high-speed marine boats.This work elaborates a new study to evaluate the hydrodynamic performance of a planing boat with a trim tab and an interceptor,and optimizes them by using an optimization algorithm.The trim tab and the interceptor have been used to optimize the running trim and motion control of semi-planing and planing boats at various speeds and sea conditions for many years.In this paper,the usage of trim tab is mathematically verified and experimental equations are utilized to optimize the performance of a planing boat at a specificd trim angle by using an optimization algorithm.The genetic algorithm(GA)is one of the most useful optimizing methods and is used in this study.The planing boat equations were programmed according to Savitsky’s equations and then analyzed in the framework of the GA-based optimization for performance improvement of theplaning hull.The optimal design of trim tab and interceptor for planing boat can be considered a multiobjective problem.The input data of GA include different parameters,such as speed,longitudinal center of gravity,and deadrise angle.We can extract the best range of forecasting the planing boat longitudinal center of gravity,the angle of the trim,and the least drag force at the best trim angle of the boat. 展开更多
关键词 Trimtab INTERCEPTOR Drag force Genetic ALGORITHM Optimization ALGORITHM
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Rol genes enhance content of artemisinin and other secondary metabolites in Shennong hybrid of Artemisia annua
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作者 Sara Zafar Erum Dilshad +2 位作者 Hammad Ismail Chahat Batool Rizvi Bushra Mirza 《中草药:英文版》 CAS 2019年第2期209-215,共7页
Objective: Artemisia annua is the chief source of artemisinin, a potent antimalarial agent, in which other bioactive phytochemicals are also present. Due to low levels of bioactive compounds including artemisinin and ... Objective: Artemisia annua is the chief source of artemisinin, a potent antimalarial agent, in which other bioactive phytochemicals are also present. Due to low levels of bioactive compounds including artemisinin and flavonoids, it is necessary to increase the level of the secondary metabolites by regulating the expression of rol genes in the plant.Methods: A hybrid variety of A. annua(Hyb1209 r, Shennong) developed by the Centre for Novel Agricultural Products, University of York, UK, was selected to produce transgenics of rolB and rolC genes. Genetic transformation was carried out via Agrobacterium tumefaciens GV3101 harboring rolB and rolC genes of Agrobacterium rhizogenes cloned separately. HPLC was used for the qualitative and quantitative analysis of flavonoids and artemisinin. Furthermore, thin layer chromatography(TLC) was also used to analyze artemisinin content.Results: Comparative analysis via HPLC revealed considerable enhancement in the phytochemical content of transgenic A. annua plants as compared to the wild type plant. Transgenics of rolB gene showed an average increase of 321% in rutin, 97.2% in caffeic acid, and 218.4% in myricetin, respectively. In the case of rolC gene transgenics, an average increase of 197.5% in rutin, 76.3% in caffeic acid, and 209.3%in myricetin was observed. Transgenics of rolB and rolC genes showed a 14.3%–28.6% and 2.8%–12.7% increase in artemisinin content respectively by HPLC analysis. TLC analysis showed that an average 142.2%and 110.2% enhancement in artemisinin for rolB and rolC transgenics respectively, compared with the wild type. An enhanced production of total flavonoids(average 30.2% and 25.5% increase in rolB and rolC transgenics, respectively) and total phenolics(average 34.3% and 25.8% increase in rolB and rolC transgenics, respectively) was observed as a result of transformation. Transformed A. annua plants showed improved free radical scavenging activity(average 46.5% and 29.1% increase in rolB and rolC transgenics,respectively) and total reducing po 展开更多
关键词 ARTEMISIA annua L. ARTEMISININ genetic transformation Hyb1209r ROL GENES
Study on scheduling algorithm for multiple handling requests of single automated guided vehicles 预览
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作者 陆远 Feng Kuikui Hu Ying 《高技术通讯:英文版》 CAS 2019年第3期334-339,共6页
To solve the problem of small amount of machining centers in small and medium flexible manufacture systems(FMS), a scheduling mode of single automated guided vehicle(AGV) is adopted to deal with multiple transport req... To solve the problem of small amount of machining centers in small and medium flexible manufacture systems(FMS), a scheduling mode of single automated guided vehicle(AGV) is adopted to deal with multiple transport requests in this paper. Firstly, a workshop scheduling mechanism of AGV is analyzed and a mathematical model is established using Genetic Algorithm. According to several sets of transport priority of AGV, processes of FMS are encoded, and fitness function, selection, crossover, and variation methods are designed. The transport priority which has the least impact on scheduling results is determined based on the simulation analysis of Genetic Algorithm, and the makespan, the longest waiting time, and optimal route of the car are calculated. According to the actual processing situation of the workshop, feasibility of this method is verified successfully to provide an effective solution to the scheduling problem of single AGV. 展开更多
关键词 AUTOMATED GUIDED vehicle(AGV) flexible manufacturing scheduling policy MAKESPAN genetic algorithm PRIORITY
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Genetic algorithm aided density functional theory simulations unravel the kinetic nature of Au(100) in catalytic CO oxidation
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作者 Yi Fang Xueqing Gong 《中国化学快报:英文版》 SCIE CAS CSCD 2019年第6期1346-1350,共5页
Heterogeneous catalysis is of tremendous importance to modern industries. Exposed atoms of heterogeneous catalysts are heavily involved in surface processes such as the adsorption, activation, diffusion and reaction o... Heterogeneous catalysis is of tremendous importance to modern industries. Exposed atoms of heterogeneous catalysts are heavily involved in surface processes such as the adsorption, activation, diffusion and reaction of substrate molecules. Surfaces of metal or metal oxide based catalysts are usually taken as hard templates that only undergo limited relaxation during catalytic reactions, especially in theoretical simulations. In this work, by using genetic algorithm (GA) aided density functional theory (DFT) calculations, we studied the surface processes involved in CO oxidation on the Au(100) surface. The use of GA greatly improves the capacity of DFT calculations in locating the potential energy surface (PES) of the surface reactions, and surprisingly, it has been found that the Au(100) surface can undergo drastic reconstruction under the influence of O adsorption and the adapted partially oxidized Au surface exhibits unique activities for subsequent adsorptions and reactions. This work depicts the kinetic nature of the Au (100) surface in its catalyzed reactions and also significantly expands our understanding of how surface atoms act in heterogeneous catalysis. 展开更多
关键词 Gold CATALYSTS GENETIC algorithm Density functional theory Potential energy SURFACE SURFACE reconstruction
Domestication of marine microalga Nannochloropsis oceanica to freshwater medium and the physiological responses 预览
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作者 GUO Li LIANG Sijie +3 位作者 ZHANG Zhongyi LIU Hang WANG Songwen YANG Guanpin 《海洋湖沼学报(英文)》 SCIE CAS CSCD 2019年第4期1353-1362,共10页
Marine microalga Nannochloropsis oceanica LAMB0001 were domesticated (~730 generations,~two days each) to adapt freshwater BG11 medium. A number of freshwater medium adapted colony-derived strains were obtained. The s... Marine microalga Nannochloropsis oceanica LAMB0001 were domesticated (~730 generations,~two days each) to adapt freshwater BG11 medium. A number of freshwater medium adapted colony-derived strains were obtained. The strains were verified phylogenetically to be N. oceanica LAMB0001 based on the 18S ribosomal RNA gene. Freshwater-medium adapted strain (FA1) grew faster in the BG11 medium prepared with freshwater than wild-type N. oceanica grew in f/2 medium prepared with seawater. We assumed that (1) the expression patterns of the genes that expressed differentially between FA1 and the wild-type N. oceanica exposing to the BG11 medium (WT-F) have been reprogrammed;(2) the physiological processes in which these genes involved have been modified;and (3) a Gene Ontology (GO) term or a KEGG pathway enriched by DEGs between FA1 and WT-F has been up- or down-regulated if it was enriched simultaneously by up- or down-regulated DEGs between FA1 and WT-F, respectively. Under these assumptions, we found that FA1 reprogrammed the expression patterns of a set of genes that involved in cell adhesion, membrane and membrane integrity, material transportation, cell movement, and cellular signaling network. These changes in cellular functions and metabolic pathways indicate that the microalga modified its gene expression pattern in a wide function range and at a high regulation rank in order to adapt to the freshwater medium. It is feasible to domesticate marine microalgae to a freshwater habitat, which may aid to modify their cultivation performances. 展开更多
关键词 NANNOCHLOROPSIS oceanica DOMESTICATION ACCLIMATION ADAPTATION genetic variation
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Did maize domestication and early spread mediate the population genetics of corn leafhopper?
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作者 Julio S. Bernal Amanda M. Davila-Flores +3 位作者 Raul F. Medina Yolanda H. Chen Kyle E. Harrison Kimberly A. Berrier 《昆虫科学:英文版》 SCIE CAS CSCD 2019年第3期569-586,共18页
Investigating how crop domestication and early farming mediated crop attributes, distributions, and interactions with antagonists may shed light on today's agricultural pest problems. Crop domestication generally ... Investigating how crop domestication and early farming mediated crop attributes, distributions, and interactions with antagonists may shed light on today's agricultural pest problems. Crop domestication generally involved artificial selection for traits desirable to early farmers, for example, in creased productivity or yield, and enhanced qualities, though invariably it altered the interactions between crops and insects, and expanded the geographical ranges of crops. Thus, some studies suggest that with crop domestication and spread, insect populations on wild crop ancestors gave rise to pestiferous insect populations on crops. Here, we addressed whether the emergence of corn leafhopper (Dalbulus ma id is) as an agricultural pest may be associated with domestication and early spread of maize (Zea mays mays). We used AFLP markers and mitochondrial COI sequences to assess population genetic structuring and haplotype relationships among corn leafhopper samples from maize and its wild relative Zea diploperennis from multiple locations in Mexico and Argentina. We uncovered seven corn leafhopper haplotypes contained within two haplogroups, one haplogroup containing haplotypes associated with maize and the other containing haplotypes associated with Z. diploperennis in a mountainous habitat. Within the first haplogroup, one haplotype was predominant across Mexican locations, and another across Argentinean locations;both were considered pestiferous. We suggested that the divergence times of the maize-associated haplogroup and of the "pestiferous" haplotypes are correlated with the chronology of maize spread following its domestication. Overall, our results support a hypothesis positing that maize domestication favored corn leafhopper genotypes preadapted for exploiting maize so that they became pestiferous, and that with the geographical expansi on of maize farming, corn leafhopper colonized Z. diploperennis, a host exclusive to secluded habitats that serves as a refuge for archaic corn leafhopper genotypic diversity. B 展开更多
关键词 Dalbulus maidis HAPLOTYPE network analysis PERENNIAL TEOSINTE population genetic structuring ZEA diploperennis ZEA mays mays
Genetic diversity in old populations of sessile oak from Calabria assessed by nuclear and chloroplast SSR
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作者 Antonio LUPINI Meriem Miyassa ACI +5 位作者 Antonio MAUCERI Giuseppe LUZZI Silvio BAGNATO Giuliano MENGUZZATO Francesco MERCATI Francesco SUNSERI 《山地科学学报:英文版》 SCIE CSCD 2019年第5期1111-1120,共10页
In the framework of forest resources conservation, this study aims to understand the dynamic and the genetic structure of sessile oak forests in Calabria, Italy. Two old populations of sessile oak(Quercus petraea(Matt... In the framework of forest resources conservation, this study aims to understand the dynamic and the genetic structure of sessile oak forests in Calabria, Italy. Two old populations of sessile oak(Quercus petraea(Mattuschka) Liebl.) from two areas of Sila and Aspromonte massifs in Calabria were analyzed for genetic diversity and population structure based on 6 nuclear simple sequence repeat(nSSR) and 4 chloroplastic SSR(cpSSR) loci. The populations displayed high amount of genetic diversity, which was toughly structured according to their geographical origins. Number of alleles at SSR loci ranged from 11 to 20 with an average of 13.5 per locus. Gene diversity(expected heterozygosity, He) estimates ranged from 0.575 to 0.834 with a mean of 0.749. The observed heterozygosity(Ho) was on average 0.458 ranging from 0.150 to 0.682. Polymorphism information content(PIC) values ranged from 0.625 to 0.865 with an average of 0.787. The analysis of molecular variance(AMOVA) highlighted a significant higher estimated variance within populations compared to among populations. Finally, the analysis of haplotypes by using cpSSR suggested a higher diversification in the population from Sila. Hierarchical clustering analysis grouped the genotypes into two major clusters, which agreed with the geographic origin of populations, and was confirmed by the Discriminant Analysis of Principal Components(DAPC). The first cluster included plants/population from Sila massif, while the second encompassed mostly plants/population sampled in Aspromonte massif. Finally, model-based clustering by STRUCTURE analysis also supported the presence of clear genetic structuring in the collection with two major populations(K=2) supported to PCoA analysis as well. Finally, our data indicated the Aspromonte population as a marginal forest with fragmented distribution suggesting different strategies of preservation than in Sila massif. 展开更多
关键词 Aspromonte and Sila massifs Genetic structure QUERCUS spp Population GENETICS Simple sequence repeat(SSR)
Autoinflammatory characteristics and short-term effects of delivering high-dose steroids to the surface of the intact endolymphatic sac and incus in refractory Meniere’s disease 预览
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作者 Jing Zou 《中华耳科学杂志:英文版》 CSCD 2019年第2期40-50,共11页
Objective:To investigate immune-related genetic background in intractable Meniere’s disease(MD)and the immediate results of a novel therapy by delivering steroids to the surface of the intact endolymphatic sac(ES)and... Objective:To investigate immune-related genetic background in intractable Meniere’s disease(MD)and the immediate results of a novel therapy by delivering steroids to the surface of the intact endolymphatic sac(ES)and incus in a sustainable manner.Case report and methods:Candidate genes involved in immune regulation were sequenced using a nextgeneration sequencing method in a patient with intractable MD.Mutations were confirmed using the Sanger sequencing method.The ES was exposed,and gelatin sponge particles were immersed in highdose methylprednisolone solution and placed onto the surface of ES.“L”-shaped gelatin sponge strips were immersed in dexamethasone solution and served as a guiding device for the steroids by touching the incus and gelatin sponge particles on the surface of the ES.Gelatin sponge particles immersed in dexamethasone solution were placed around the gelatin sponge strips and sealed using fibrin glue.Results:Autoinflammation in the refractory MD case was indicated by genotype,including novel heterozygous mutations of PRF1,UNC13D,SLC29A3,ITCH,and JAK3,as well as phenotype.The vertigo was fully relieved immediately after operation.Tinnitus and aural fullness were resolved 3 weeks after operation,whereas hearing improved in 2 mon postoperation.No recurrence was noted during the 5-monfollow-up,and the final MRI supported the novel therapeutic hypothesis.Conclusion:Autoinflammation was involved in a refractory MD.This novel therapy,which involves the delivery of steroids to the surface of the intact ES and incus,is effective in relieving vertigo and tinnitus and improves hearing function of refractory MD. 展开更多
关键词 VESTIBULAR function TINNITUS Genetic disorders Hearing
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GPA:A Microbial Genetic Polymorphisms Assignments Tool in Metagenomic Analysis by Bayesian Estimation
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作者 Jiarui Li Pengcheng Du +4 位作者 Adam Yongxin Ye Yuanyuan Zhang Chuan Song Hui Zeng Chen Chen 《基因组蛋白质组与生物信息学报:英文版》 CAS CSCD 2019年第1期106-117,共12页
Identifying antimicrobial resistant(AMR)bacteria in metagenomics samples is essential for public health and food safety.Next-generation sequencing(NGS)technology has provided a powerful tool in identifying the genetic... Identifying antimicrobial resistant(AMR)bacteria in metagenomics samples is essential for public health and food safety.Next-generation sequencing(NGS)technology has provided a powerful tool in identifying the genetic variation and constructing the correlations between genotype and phenotype in humans and other species.However,for complex bacterial samples,there lacks a powerful bioinformatic tool to identify genetic polymorphisms or copy number variations(CNVs)for given genes.Here we provide a Bayesian framework for genotype estimation for mixtures of multiple bacteria,named as Genetic Polymorphisms Assignments(GPA).Simulation results showed that GPA has reduced the false discovery rate(FDR)and mean absolute error(MAE)in CNV and single nucleotide variant(SNV)identification.This framework was validated by whole-genome sequencing and Pool-seq data from Klebsiella pneumoniae with multiple bacteria mixture models,and showed the high accuracy in the allele fraction detections of CNVs and SNVs in AMR genes between two populations.The quantitative study on the changes of AMR genes fraction between two samples showed a good consistency with the AMR pattern observed in the individual strains.Also,the framework together with the genome annotation and population comparison tools has been integrated into an application,which could provide a complete solution for AMR gene identification and quantification in unculturable clinical samples.The GPA package is available at https://github.com/IID-DTH/GPA-package. 展开更多
关键词 Next-generation sequencing Pool-seq Bayesian model METAGENOMICS Genetic polymorphisms
Effects of SLCO1 Bl and GATM gene variants on rosuvastatin-induced myopathy are unrelated to high plasma exposure of rosuvastatin and its metabolites
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作者 Xue Bai Bin Zhang +8 位作者 Ping Wang Guan-lei Wang Jia-li Li Ding-sheng Wen Xing-zhen Long Hong-shuo Sun Yi-bin Liu Min Huang Shi-Iong Zhong 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第4期492-499,共8页
Myotoxicity is a sign ificant factor contributi ng to the poor adhere nee and reduced effective ness in the treatme nt of statins. Gen etic variations and high drug plasma exposure are considered as critique causes fo... Myotoxicity is a sign ificant factor contributi ng to the poor adhere nee and reduced effective ness in the treatme nt of statins. Gen etic variations and high drug plasma exposure are considered as critique causes for statin-induced myopathy (SIM). This study aims to explore the sequential influences of rosuvastatin (RST) pharmacokinetic and myopathy-related single-nucleotide polymorphisms (SNPs) on the plasma exposure to RST and its metabolites: rosuvastatin lactone (RSTL) and /V-desmethyl rosuvastatin (DM-RST), and further on RST-induced myopathy. A total of 758 Chinese patients with coronary artery disease were enrolled and followed up SIM incidents for 2 years. The plasma concentrations of RST and its metabolites were determined through a validated ultra-performanee liquid chromatography mass spectrometry method. Nine SNPs in six genes were genotyped by using the Sequenom MassArray iPlex platform. Results revealed that ABCG2 rs2231142 variations were highly associated with the plasma concentrations of RST, RSTL, and DM-RST (Pad)<0.01, FDR < 0.05). CYP2C9 rs1057910 significantly affected the DM-RST concentration (Padj <0.01, FDR < 0.05). SLCO1B1 rs4149056 variant allele was significantly associated with high SIM risk (OR: 1.741, 95% Cl: 1.180-2.568, P = 0.0052, FDR = 0.0468). Glycine amidinotransferase (GATM) rs9806699 was marginally associated with SIM incidents (OR: 0.617, 95% Cl: 0.406-0.939, P = 0.0240, FDR = 0.0960). The plasma concentrations of RST and its metabolites were not significantly different between the SIM (n = 51) and control groups (n=707)(all P > 0.05). In conclusion, SLCO1B1 and GATM genetic variants are potential biomarkers for predicting RST-induced myopathy, and their effects on SIM are unrelated to the high plasma exposure of RST and its metabolites. 展开更多
关键词 genetic POLYMORPHISM ROSUVASTATIN METABOLITES PLASMA concentration MYOPATHY
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