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Blessing and a curse of outpatient management of delayed graft function 预览
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作者 Justin W Blazel Jennifer A Turk +1 位作者 Brenda L Muth Sandesh Parajuli 《世界移植杂志》 2019年第4期58-61,共4页
Delayed graft function (DGF) is a common complication occurring most often after deceased donor kidney transplant with several donor characteristics as well as immunologic factors that lead to its development post-tra... Delayed graft function (DGF) is a common complication occurring most often after deceased donor kidney transplant with several donor characteristics as well as immunologic factors that lead to its development post-transplant.These patients require dialysis and close kidney function monitoring until sufficient allograft function is achieved.This has resulted in limited options for DGF management,either prolonged hospitalization until graft function improves to the point where dialysis is no longer needed or discharge back to their home dialysis unit with periodic follow up in the transplant clinic.DGF is associated with a higher risk for acute rejection,premature graft failure,and 30-d readmission;therefore,these patients need close monitoring,immunosuppression management,and prompt allograft biopsy if prolonged DGF is observed.This may not occur if these patients are discharged back to their home dialysis unit.To address this issue,the University of Wisconsin-Madison created a clinic in 2011 specialized in outpatient DGF management.This clinic was able to successfully reduce hospital length of stay without an increase in 30-d readmission,graft loss,and patient death. 展开更多
关键词 Delayed graft function KIDNEY transplantation IMMUNOSUPPRESSION Acute rejection KIDNEY DONOR profile INDEX KIDNEY DONOR risk INDEX Dialysis
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Histological and clinical evaluation of marginal donor kidneys before transplantation:Which is best? 预览
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作者 Maurizio Salvadori Aris Tsalouchos 《世界移植杂志》 2019年第4期62-80,共19页
Organ shortage represents one of the major limitations to the development of kidney transplantation.To increase the donor pool and to answer the ever increasing kidney request,physicians are recurring to marginal kidn... Organ shortage represents one of the major limitations to the development of kidney transplantation.To increase the donor pool and to answer the ever increasing kidney request,physicians are recurring to marginal kidneys as kidneys from older donors,from hypertensive or diabetic donors and from nonheart beating donors.These kidneys are known to have frequently a worse outcome in the recipients.To date major problem is to evaluate such kidneys in order to use or to discard them before transplantation.The use of such kidneys create other relevant question as whether to use them as single or dual transplant and to allocate them fairly according transplant programs.The pre-transplant histological evaluation,the clinical evaluation of the donor or both the criteria joined has been used and according the time each criterion prevailed over the others.Aim of this review has been to examine the advantages and the drawbacks of any criterion and how they have changed with time.To date any criterion has several limitations and several authors have argued for the development of new guidelines in the field of the kidney evaluation for transplantation.Several authors argue that the use of omic technologies should improve the organ evaluation and studies are ongoing to evaluate these technologies either in the donor urine or in the biopsies taken before transplantation. 展开更多
关键词 KIDNEY EVALUATION Pre-transplant BIOPSIES KIDNEY DONOR EVALUATION KIDNEY RISK profile index Omic technologies Deceased DONOR SCORE DONOR RISK SCORE
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试论“血之源头在乎肾”
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作者 崔家康 姜泉 《中医学报》 CAS 2019年第4期686-688,共3页
血在脉道中运行,流注周身,循环不已,维持人体正常的生理活动。肾为先天之本,肾主藏精,肾阴肾阳为五脏阴阳之本,其在血生成的过程中起着非常重要的作用,肾也影响着血行,故有'血之源头在乎肾'之说。肾精、肾气、肾阴、肾阳在血化... 血在脉道中运行,流注周身,循环不已,维持人体正常的生理活动。肾为先天之本,肾主藏精,肾阴肾阳为五脏阴阳之本,其在血生成的过程中起着非常重要的作用,肾也影响着血行,故有'血之源头在乎肾'之说。肾精、肾气、肾阴、肾阳在血化生的过程中起着关键的作用,肾是血化生的本原和动力,血液运行同样与肾关系密切,肾虚可导致血虚,亦可引起瘀血、出血等病理改变。在治疗上,当遵'损其肾者益其精''形不足者,温之以气;精不足者,补之以味'的原则,具体方法有填肾精益精髓而资血、温肾阳益肾气而养血、平补肾阴肾阳而生血等。 展开更多
关键词 肾精 肾阴 血行
京尼平交联大鼠肾去细胞生物支架提高支架生物学性能的实验研究
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作者 刘丹 杜奥玲 +4 位作者 王新旺 张德明 唐汇李 王宇航 陈胜华 《中国临床解剖学杂志》 CSCD 北大核心 2019年第1期71-76,共6页
目的通过京尼平交联大鼠肾去细胞生物支架,提高支架的生物学性能。方法取250 g左右的健康SD大鼠80只,分为正常组、未交联支架组、戊二醛交联支架组和京尼平交联支架组。游离大鼠肾、肾动脉,连接蠕动泵,经PBS灌注去血后得到的肾作为正常... 目的通过京尼平交联大鼠肾去细胞生物支架,提高支架的生物学性能。方法取250 g左右的健康SD大鼠80只,分为正常组、未交联支架组、戊二醛交联支架组和京尼平交联支架组。游离大鼠肾、肾动脉,连接蠕动泵,经PBS灌注去血后得到的肾作为正常组。其余大鼠肾依次灌入肝素化PBS溶液、1%TritonX-100、1%十二烷基硫酸钠(SDS)、去离子水,完成大鼠肾去细胞生物支架制备。戊二醛交联支架组继续灌入0.625%戊二醛(GA)1500 mL;京尼平交联支架组浸入0.5%的京尼平溶液于37℃恒温箱中行化学交联24 h;未经戊二醛或京尼平交联的肾去细胞支架作为未交联支架组。对4组肾分别作HE、Masson、免疫荧光染色及电子显微镜扫描,观察支架组织形态学及超微结构改变;力学拉伸试验检测机械力学性能。结果 SD大鼠肾支架经京尼平交联后,HE、Masson染色显示胶原纤维排列更加紧密有序,肾小球处的纤维呈聚集状,Collagen Ⅰ和Collagen Ⅳ荧光染色增强,电镜扫描可见交联后的去细胞支架内蜂窝状孔洞结构更加立体,并可见典型的肾小球龛样结构轮廓更加清晰;交联组肾弹性模量较支架组明显增强。结论京尼平交联大鼠肾支架能提高支架的生物学性能有助于为后期细胞植入和器官再生。 展开更多
关键词 京尼平 戊二醛 生物支架 肾再生
Shen-Jing as a Chinese Medicine Concept Might Be a Counterpart of Stem Cells in Regenerative Medicine
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作者 REN Yan-bo HUANG Jian-hua +1 位作者 CAI Wai-jiao SHEN Zi-yin 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第1期64-70,共7页
As the epitome of the modern regenerative medicine, stem cells were proposed in the basic sense no more than 200 years ago. However, the concept of "stem cells" existed long before the modern medical descrip... As the epitome of the modern regenerative medicine, stem cells were proposed in the basic sense no more than 200 years ago. However, the concept of "stem cells" existed long before the modern medical description. The hypothesis that all things, including our sentient body, were generated from a small origin was shared between Western and Chinese people. The ancient Chinese philosophers considered Jing(also known as essence) as the origin of life. In Chinese medicine(CM), Jing is mainly stored in Kidney(Shen) and the so-called Shen-Jing(Kidney essence). Here, we propose that Shen-Jing is the CM term used to express the meaning of "origin and regeneration". This theoretical discovery has at least two applications. First, the actions underlying causing Shen-Jing deficiency, such as excess sexual intercourse, chronic diseases, and aging, might damage the function of stem cells. Second, a large number of Chinese herbs with Shen-Jing-nourishing efficacy had been proven to affect stem cell proliferation and differentiation. Therefore, if Shen-Jing in CM is equivalent with stem cells in regenerative medicine, higher effective modulators for regulating stem-cell behaviors from Kidney-tonifying herbs would be expected. 展开更多
关键词 Shen-Jing KIDNEY essence KIDNEY tonifying HERBS REGENERATIVE medicine stem cells
虫草素抗肾纤维化研究进展 预览
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作者 代丽娟 宋立群 +3 位作者 贠捷 张德欣 于思明 马艳春 《中国临床保健杂志》 CAS 2019年第1期115-119,共5页
虫草素是从蛹虫草中分离出的一种核苷类抗生素,具有明显的抗肾纤维化作用。通过对肾纤维化病理机制的研究,阐述虫草素可从细胞分子学水平调控多种细胞因子,从而介导七大信号通路,阻碍其信号传导。七种通路相互关联,从而延缓肾纤维化及... 虫草素是从蛹虫草中分离出的一种核苷类抗生素,具有明显的抗肾纤维化作用。通过对肾纤维化病理机制的研究,阐述虫草素可从细胞分子学水平调控多种细胞因子,从而介导七大信号通路,阻碍其信号传导。七种通路相互关联,从而延缓肾纤维化及慢性肾脏病的进展。 展开更多
关键词 虫草素 肾纤维化 肾功能衰竭 慢性 信号传导
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基于妇科生殖疾病古方探讨肾主生殖理论 预览
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作者 杨阳 许昕 +2 位作者 李园白 王静 陈广坤 《世界科学技术:中医药现代化》 CSCD 北大核心 2019年第2期319-326,共8页
目的:基于调治妇科生殖疾病古方,分析用药规律特点,探讨肾主生殖理论。方法:筛选调治妇科生殖疾病的古方,基于关联分析方法,统计分析高频单味药、药物组和关联药物,分析性味归经、功效等规律特点,结合妇科生殖疾病特点探讨肾主生殖理论... 目的:基于调治妇科生殖疾病古方,分析用药规律特点,探讨肾主生殖理论。方法:筛选调治妇科生殖疾病的古方,基于关联分析方法,统计分析高频单味药、药物组和关联药物,分析性味归经、功效等规律特点,结合妇科生殖疾病特点探讨肾主生殖理论。结果:共收集2679首方剂,涉及616味中药,调治生殖疾病的药物以补益气血、滋补肝肾、补肾填精、疏肝理气为主,药性偏温热;药味以甘为主,归经以脾、肾、肝经为主;妇科生殖疾病多精血气血亏虚,有气滞、痰湿的病理存在。古方多从补肾填精直接补益生殖器官;通过补益气血,滋养后天之本脾胃,间接调补生殖机能;通过肝脏疏泄精血、调节脾肾,调治生殖疾病与经产胎孕。在单味药、单方、药物组和关联药物组中,调补功效占据最多,也说明调补肾脏对生殖疾病的影响。结论:古方药物的数据挖掘,佐证了肾主生殖理论的重要性,肾脏发挥主生殖功效,离不开其他脏腑的协同作用。 展开更多
关键词 生殖 古方 肾主生殖
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“天癸”辨析
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作者 易黎 武润哲 白玲玲 《河南中医》 2019年第5期665-667,共3页
纵观《黄帝内经》全书,天癸极少涉及相关疾病,可以推测,天癸的功能较肾的功能更加稳定,不会因妊娠、胎产、哺乳情况而受本质影响或者停止,其作用会一直延续至天癸竭时。天癸是能代表"真元之气"在"肾中癸水"作用的物... 纵观《黄帝内经》全书,天癸极少涉及相关疾病,可以推测,天癸的功能较肾的功能更加稳定,不会因妊娠、胎产、哺乳情况而受本质影响或者停止,其作用会一直延续至天癸竭时。天癸是能代表"真元之气"在"肾中癸水"作用的物质,属于器官层次,主要作用是主导生殖系统功能,在先天物质化生阶段也发挥一定作用,其主导的以月经周期为核心的生理活动较为稳定,开阖有度,有术数规律。影响因素上,短期的饮食失序、劳逸不当、情志不遂不会影响到天癸的稳定活动,时间是目前所认识到的唯一因素。 展开更多
关键词 天癸 《黄帝内经》 补肾药
Pancreatic transplantation:Brief review of the current evidence 预览
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作者 Ahmed Aref Tariq Zayan +2 位作者 Ravi Pararajasingam Ajay Sharma Ahmed Halawa 《世界移植杂志》 2019年第4期81-93,共13页
Kidney transplantation is the treatment of choice for management of end-stage renal disease.However,in diabetic patients,the underlying metabolic disturbance will persist and even may get worse after isolated kidney t... Kidney transplantation is the treatment of choice for management of end-stage renal disease.However,in diabetic patients,the underlying metabolic disturbance will persist and even may get worse after isolated kidney transplantation.Pancreatic transplantation in humans was first introduced in 1966.The initial outcome was disappointing.However,this was changed after the improvement of surgical techniques together with better patient selection and the availability of potent and better-tolerated immune-suppression like cyclosporine and induction antibodies.Combined kidney and pancreas transplantation will not only solve the problem of organ failure,but it will also stabilise or even reverse the metabolic complications of diabetes.Combined kidney and pancreas transplantation have the best long term outcome in diabetic cases with renal failure.Nevertheless,at the cost of an initial increase in morbidity and risk of mortality.Other transplantation options include pancreas after kidney transplantation and islet cell transplantation.We aim by this work to explore various options which can be offered to a diabetic patient with advanced chronic kidney disease.Our work will provide a simplified,yet up-to-date information regarding the different management options for those diabetic chronic kidney failure patients. 展开更多
关键词 Combined KIDNEY pancrease TRANSPLANTATION RENAL TRANSPLANTATION DIABETIC KIDNEY disease DIABETES MELLITUS
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Roles of short-chain fatty acids in kidney diseases
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作者 Ling-Zhi Li Si-Bei Tao +1 位作者 Liang Ma Ping Fu 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第10期1228-1232,共5页
Objective: In kidney diseases, uncontrolled blood pressure, inflammation, oxidative stress, imbalanced immunity response, metabolic dysfunction were associated with the progressive deterioration of renal function. Sho... Objective: In kidney diseases, uncontrolled blood pressure, inflammation, oxidative stress, imbalanced immunity response, metabolic dysfunction were associated with the progressive deterioration of renal function. Short-chain fatty acids (SCFAs), as a group of metabolites fermented by gut microbiota exerted regulatory effects on kidney diseases through their activation of transmembrane G protein-coupled receptors and their inhibition of histone acetylation. In this review article, we updated recent research advances that provided an opportunity to explore our understanding in physiology and function of SCFAs in kidney disease. Data sources: We performed a comprehensive search in both PubMed and Embase using short-chain fatty acids55 and "kidney" with no restrictions on publication date. Study selection: After reading through the title and abstract for early screening, the full text of relevant studies was identified and reviewed to summarize the roles of SCFAs in kidney diseases. Results: Though controversial, growing evidence suggested SCFAs appeared to have a complex but yet poorly understood communications with cellular and molecular processes that affected kidney function and responses to injury. From recent studies, SCFAs influenced multiple aspects of renal physiology including inflammation and immunity, fibrosis, blood pressure, and energy metabolism. Conclusions: The roles of intestinal SCFAs in kidney diseases were exciting regions in recent years;however, clinical trials and animal experiments in kidney diseases were still lacked. Thus, more research would be needed to obtain better understanding of SCFAs' potential effects in kidney diseases. 展开更多
关键词 GUT MICROBIOME Short-chain FATTY acid KIDNEY disease Gut-kidney AXIS
从肾论治变应性鼻炎 被引量:1
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作者 潘梦晨 王东方 《中医学报》 CAS 2019年第2期266-269,共4页
肾脏与变应性鼻炎关系密切。肾藏精,主水,主纳气,为气之根,五脏阴阳之本,肾气充足、肾阳充盛,则鼻窍通利、嗅觉聪敏、呼吸顺畅。肾失温煦,纳气无权以及水液代谢功能障碍,则鼻失温养、清道壅塞、喷嚏频频、清涕量多,其关键在于肾阳亏虚... 肾脏与变应性鼻炎关系密切。肾藏精,主水,主纳气,为气之根,五脏阴阳之本,肾气充足、肾阳充盛,则鼻窍通利、嗅觉聪敏、呼吸顺畅。肾失温煦,纳气无权以及水液代谢功能障碍,则鼻失温养、清道壅塞、喷嚏频频、清涕量多,其关键在于肾阳亏虚。此外,足少阴肾经与鼻有着间接的经络联系,肾与变应性鼻炎在现代病理学和免疫学方面均有相关性。变应性鼻炎临床遣方用药当重视“补肾固本,益气通窍”之法. 展开更多
关键词 变应性鼻炎 补肾固本 益气通窍
Progress of small ubiquitin-related modifiers in kidney diseases
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作者 Ou Li Qian Ma +3 位作者 Fei Li Guang-Yan Cai Xiang-Mei Chen Quan Hong 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第4期466-473,共8页
Objective: Small ubiquitin-related modifiers (SUMOs) are a group of post-translational modification proteins extensively expressed in eukaryotes. Abnormal SUMOylation can lead to the development of various diseases. T... Objective: Small ubiquitin-related modifiers (SUMOs) are a group of post-translational modification proteins extensively expressed in eukaryotes. Abnormal SUMOylation can lead to the development of various diseases. This article summarizes the progress on research of the role of SUMOs in various types of kidney diseases to further increase the understanding of the regulatory functions of SUMOylation in the pathogenesis of kidney diseases. Data sources: This review was based on articles published in the PubMed databases up to January 2018, using the keywords including "SUMOs," "SUMOylation," and "kidney diseases." Study selection: Original articles and critical reviews about SUMOs and kidney disease were selected for this review. A total of 50 studies were in English. Results: SUMO participates in the activation of NF-κB inflammatory signaling pathway, playing a central regulatory role in the inflammation and progression of DN, and the secretion of various chemokines in AKI. SUMO involves in the regulation of TG2 and Nrf2 antioxidant stress, affecting renal tubular injury in AKI. SUMO affects the MAPK/ERK pathway, regulating intracellular signal transduction, modulating the transcription and expression of effector molecules in DN. SUMO contributes to the TGF-β/Smad pathway, leading to fibrosis of the kidney. The conjugate combination of SUMO and p53 regulates cell proliferation and apoptosis, and participates in the regulation of tumorigenesis. In addition, SUMOylation of MITF modulates renal tumors secondary to melanoma, Similarly, SUMOylation of tumor suppressor gene VHL regulates the occurrence of renal cell carcinoma in VHL syndrome. Conclusions: Tissue injury, inflammatory responses, fibrosis, apoptosis, and tumor proliferation in kidney diseases all involve SUMOs. Further research of the substrate SUMOylation and regulatory mechanisms of SUMO in kidney diseases will improve and develop new treatment measures and strategies targeting kidney diseases. 展开更多
关键词 SMALL ubiquitin-related MODIFIERS SUMOYLATION KIDNEY diseases
当归补血汤对肾缺血再灌注损伤大鼠IL-12、IL-17和IL-18的影响 预览
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作者 倪文娟 刘福和 +1 位作者 王国康 俞松林 《中国中医药科技》 CAS 2019年第2期180-185,共6页
目的:探讨当归补血汤对肾缺血再灌注损伤大鼠血清和肾组织白细胞介素-12(IL-12)、白细胞介素-17(IL-17)和白细胞介素-18(IL-18)的影响。方法:共选取45只SD大鼠,以随机数字表法分为3组,每组各15只;假手术组(Sham组)、缺血再灌注模型对照... 目的:探讨当归补血汤对肾缺血再灌注损伤大鼠血清和肾组织白细胞介素-12(IL-12)、白细胞介素-17(IL-17)和白细胞介素-18(IL-18)的影响。方法:共选取45只SD大鼠,以随机数字表法分为3组,每组各15只;假手术组(Sham组)、缺血再灌注模型对照组(IR组)、当归补血汤预处理组(IR+DG组);各组大鼠给予相应处理5 d,夹闭左肾动脉45 min,再灌注2 h造模,采用ELISA法测定造模后1、3、6、12、24、48 h不同时点血清IL-12、IL-17和IL-18的含量,采用免疫组化方法检测肾组织IL-12、IL-17和IL-18表达。结果:造模后1、3、6、12、24、48 h不同时点血清IL-12、IL-17和IL-18的含量在I/R和I/R+DG组中均明显高于Sham组;在I/R组和I/R+DG组中,IL-12、IL-17和IL-18的表达随病程的进展而升高,但是I/R组明显高于I/R+DG组。免疫组化检测结果显示IL-12、IL-17和IL-18在Sham组肾组织中无表达,在IR组的表达明显升高,IL-12、IL-17和IL-18的表达程度均明显高于IR+DG。结论:当归补血汤可以减少肾I/R病理过程中促炎性细胞因子IL-12、IL-17和IL-18的产生,有助于减轻I/R时炎性细胞因子瀑布样级联反应,发挥抗炎作用,减轻肾缺血再灌注性的损伤,保护肾功能。 展开更多
关键词 当归补血汤 缺血再灌注 白细胞介素-12 白细胞介素质-17 白细胞介素-18 大鼠
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Lump type crossed fused renal ectopia with bilateral vesicoureteral reflux: A case report 预览
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作者 Taesoo Choi Koo Han Yoo +1 位作者 Ran Song Dong-Gi Lee 《世界临床病例杂志》 2019年第6期773-777,共5页
BACKGROUND Crossed fused renal ectopia is a rare congenital anomaly of the ascent of the kidney. This anomaly may be observed as a solitary kidney during initial evaluation. A solitary kidney must be evaluated for ass... BACKGROUND Crossed fused renal ectopia is a rare congenital anomaly of the ascent of the kidney. This anomaly may be observed as a solitary kidney during initial evaluation. A solitary kidney must be evaluated for associated anomalies such as duplication, horseshoe kidney, or crossed renal ectopia. CASE SUMMARY An anomaly was observed in a 9-mo-old male child who was subsequently diagnosed with crossed fused renal ectopia and vesicoureteral reflux (VUR). In this condition, recurrent febrile urinary tract infection can be a serious problem, and can easily cause renal damage due to relatively short ureters and high pressure in the kidney. CONCLUSION To prevent urosepsis and preserve renal function, early diagnosis and proper management including surgical correction should be considered for the management of renal ectopia with VUR. 展开更多
关键词 Kidney CONGENITAL ABNORMALITIES Vesicoureteral REFLUX Ureteroneocystostomy Politano-Leadbetter Case report
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蝙蝠蛾拟青霉菌Cs-4菌株对腺嘌呤造成肾脏和睾丸损伤的恢复作用
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作者 闫俊 包海鹰 +3 位作者 闫芳 杨明 图力古尔 李玉 《菌物学报》 CAS CSCD 北大核心 2019年第6期926-937,共12页
本文用腺嘌呤建立肾脏和睾丸损伤的小鼠模型,探索蝙蝠蛾拟青霉菌Cs-4菌株对腺嘌呤造成的肾脏和睾丸损伤的恢复作用。将小鼠随机分为空白组、模型组和蝙蝠蛾拟青霉菌Cs-4菌粉的5个剂量组(350mg/kg、550mg/kg、750mg/kg、1 500mg/kg、3 00... 本文用腺嘌呤建立肾脏和睾丸损伤的小鼠模型,探索蝙蝠蛾拟青霉菌Cs-4菌株对腺嘌呤造成的肾脏和睾丸损伤的恢复作用。将小鼠随机分为空白组、模型组和蝙蝠蛾拟青霉菌Cs-4菌粉的5个剂量组(350mg/kg、550mg/kg、750mg/kg、1 500mg/kg、3 000mg/kg),连续给予相应药物21d,对各组小鼠的肾脏、睾丸通过HE染色、Masson染色,进行组织病理学检查,测定各组小鼠血清中尿素氮(BUN)、肌酐(Cr)、促卵泡刺激素(FSH)、黄体生成素(LH)含量,测定睾丸组织匀浆中的酸性磷酸酶(ACP)、琥珀酸脱氢酶(SDH)和乳酸脱氢酶(LDH)水平。结果表明,蝙蝠蛾拟青霉菌Cs-4菌株具有使小鼠体重逐渐恢复上升趋势、使受损的肾脏和睾丸组织结构恢复的作用,能使BUN、Cr、FSH、LH、ACP、SDH和LDH水平趋于正常化。 展开更多
关键词 蝙蝠蛾拟青霉菌Cs-4菌株 腺嘌呤 肾脏 睾丸 恢复作用
酵母源金属硫蛋白对慢性汞中毒小鼠排汞及肾脏损伤的修复作用
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作者 王欣卉 徐炳政 王颖 《中国食品学报》 EI CAS CSCD 北大核心 2019年第1期41-47,共7页
探讨具有自主知识产权的酵母源金属硫蛋白(MT-1、MT-2)对慢性汞中毒小鼠排汞及汞致肾脏损伤修复作用。SPF级昆明雄性种鼠经氯化汞染毒,灌胃酵母源金属硫蛋白及二巯基丙磺酸钠(DMPS)28 d后测定小鼠体质量、肾脏湿重及肾指数、血液及肾脏... 探讨具有自主知识产权的酵母源金属硫蛋白(MT-1、MT-2)对慢性汞中毒小鼠排汞及汞致肾脏损伤修复作用。SPF级昆明雄性种鼠经氯化汞染毒,灌胃酵母源金属硫蛋白及二巯基丙磺酸钠(DMPS)28 d后测定小鼠体质量、肾脏湿重及肾指数、血液及肾脏汞离子含量、血清尿素氮(BUN)、肌酐(CREA)指标水平,并取肾脏组织制作HE病理学染色切片,观察肾脏病变程度及灌胃药物对肾脏组织的修复情况。试验结果表明,相较于正常对照组,模型对照组小鼠体质量显著下降(P<0.05),肾指数、血液及肾脏汞含量、BUN及CREA水平显著升高(P<0.05)。HE染色切片显示模型对照组小鼠肾脏组织出现肾小球及系膜细胞增生,并伴有大量炎性细胞浸润及组织坏死现象。灌胃给药28 d后,各灌胃给药组小鼠体质量较模型对照组显著增加(P<0.05),酵母源金属硫蛋白高剂量及DMPS给药组小鼠肾指数、血液及肾脏汞含量、BUN及CREA水平显著下降(P<0.05),且病理学切片显示酵母源金属硫蛋白高剂量给药可修复小鼠肾脏受损组织细胞,水肿增生及坏死现象明显减轻。两种酵母源金属硫蛋白对慢性汞中毒小鼠具有良好的排汞及修复汞致小鼠肾脏损伤的作用。 展开更多
关键词 酵母源金属硫蛋白 汞离子 排汞 肾脏 修复
楤木总皂苷对2型糖尿病模型小鼠肾功能及肾脏细胞凋亡的影响
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作者 曾德望 李红艳 +4 位作者 赵世莉 杨椹 张云芳 李文豪 田鲁 《国际中医中药杂志》 2019年第4期381-386,共6页
目的研究楤木总皂苷对T2DM小鼠血糖及肾功能的影响,并从抗氧化及细胞凋亡角度探讨其作用机制。方法将90只雄性小鼠按随机数字表法分为正常组、模型组、阳性对照组及楤木总皂苷低、中、高剂量组。除正常组外,其余各组小鼠采用高糖高脂饲... 目的研究楤木总皂苷对T2DM小鼠血糖及肾功能的影响,并从抗氧化及细胞凋亡角度探讨其作用机制。方法将90只雄性小鼠按随机数字表法分为正常组、模型组、阳性对照组及楤木总皂苷低、中、高剂量组。除正常组外,其余各组小鼠采用高糖高脂饲料1个月及一次性大剂量注射STZ诱导T2DM小鼠模型。正常组和模型组灌胃等体积生理盐水,阳性对照组灌胃贝那普利溶液1 mg/kg,低、中和高剂量组灌胃楤木总皂苷30、60、120 mg/kg,按1 ml/kg小鼠体重灌胃,1次/d,连续灌胃6周后取材。检测小鼠血清胰岛素和SOD、MDA水平,检测小鼠尿液肌酐(creatinine, Cr)、尿素氮(urea nitrogen, UN)、尿酸(uric acid, UA)水平,采用免疫组化染色和Western blot检测肾组织中Bax和Bcl-2蛋白表达。结果与模型组比较,楤木总皂苷低、中和高剂量组小鼠血糖和胰岛素抵抗指数均明显降低(P<0.05);小鼠尿液UN、Cr和UA水平降低(P<0.05);血清SOD升高,MDA水平降低(P<0.05);肾组织Bcl-2平均灰度值[(92.26±11.36)、(107.17±9.26)、(132.65±8.45)比(56.42±16.24)]升高,Bax平均灰度值[(152.62±9.86)、(124.48±10.36)、(92.29±10.10)比(171.38±15.18)]降低(P<0.05);Bax蛋白[(0.81±0.06)、(0.75±0.07)、(0.52±0.09)比(2.02±0.09)]表达降低、Bcl-2蛋白[(0.92±0.08)、(0.94±0.12)、(1.27±0.07)比(0.30±0.09)]表达升高(P<0.05)。结论楤木总皂苷可降低T2DM小鼠血糖水平,改善肾功能,其作用机制可能与提高抗氧化能力、抑制肾脏细胞凋亡有关。 展开更多
关键词 楤木 皂苷类 糖尿病 2型 肾脏 氧化性应激 细胞凋亡 小鼠
子痫前期发病机制与临床治疗研究进展 预览
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作者 李可 朱大伟 +4 位作者 陈建昆 韩健 郑秀慧 郭建新 李力 《解放军医学杂志》 CAS CSCD 北大核心 2019年第5期423-429,共7页
子痫前期(PE)是妊娠期的一种严重并发症,全球发病率为2%~8%,是导致妊娠期妇女与围产儿发病和死亡的重要原因之一。其主要临床表现为高血压(收缩压>140 mmHg或舒张压>90 mmHg)、蛋白尿(>0.3 g/24 h),可同时伴有肾功能不全、血... 子痫前期(PE)是妊娠期的一种严重并发症,全球发病率为2%~8%,是导致妊娠期妇女与围产儿发病和死亡的重要原因之一。其主要临床表现为高血压(收缩压>140 mmHg或舒张压>90 mmHg)、蛋白尿(>0.3 g/24 h),可同时伴有肾功能不全、血小板减少、肝功能障碍、肺水肿等多系统多器官受累。抗磷脂抗体综合征、高血压、糖尿病、慢性肾病、肥胖、PE家族史、多胎妊娠、孕产妇高龄等是导致PE的危险因素。目前,PE的发病机制尚不完全清楚,但是越来越多的证据表明,血管生成因子水平异常和凝血功能障碍是引发该病的主要原因。该文主要就近年来PE发病机制及临床治疗的研究进展加以综述,以供临床借鉴。 展开更多
关键词 子痫前期 发病机制 内皮细胞 胎盘生长因子 可溶性血管内皮生长因子受体 可溶性内皮因子 肾脏
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丹红注射液对老龄家兔肾脏缺血再灌注损伤的保护作用
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作者 李天舒 闻宇 +1 位作者 吕文伟 张丽君 《国际老年医学杂志》 2019年第4期193-195,244共4页
目的探讨丹红注射液对老龄家兔肾脏缺血再灌注损伤的保护作用及可能机制。方法阻断双侧肾动脉1h,开放2h造成家兔肾脏缺血再灌注损伤。选取造模成功的家兔分为4组,分别为假于术组(F组)、缺血再灌注组(I组)、丹红注射液10ml/kg组(D1组)和2... 目的探讨丹红注射液对老龄家兔肾脏缺血再灌注损伤的保护作用及可能机制。方法阻断双侧肾动脉1h,开放2h造成家兔肾脏缺血再灌注损伤。选取造模成功的家兔分为4组,分别为假于术组(F组)、缺血再灌注组(I组)、丹红注射液10ml/kg组(D1组)和20ml/kg组(D2组)。检测血清氧化应激指标丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px);肾脏功能指标肌肝(Cr)及血尿素氮(BUN);炎症因子白介素-1(IL-1)和肿瘤坏死因子-α(TNF-α)含量水平。检测肾脏组织内皮素-1(ET-1)水平。结果与1组相比,D1及D2组的血清氧化应激指标MDA明显降低(P<0.05),而SOD、GSH、GSH-Px明显增高(P<0.05);肾功能指标Cr及BUN明显降低(P<0.05);D2组的炎症因子IL-1及TNE-α明显降低(P<0.05);D1及D2组肾组织ET-1的含量明显降低(P<0.05)。结论丹红注射液可以减轻家兔肾脏缺血再灌注损伤的氧化应激,保护肾脏功能、可能与降低炎症因子及内皮素-1的含量有关。 展开更多
关键词 丹红注射液 肾脏 缺血再灌注损伤 氧化应激 肾功能 炎症因子
Point of care renal ultrasonography for the busy nephrologist: A pictorial review 预览
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作者 Abhilash Koratala Deepti Bhattacharya Amir Kazory 《世界肾病学杂志:英文版》 2019年第3期44-58,共15页
The application of bedside ultrasonography in routine clinical practice has dramatically evolved over the last few decades and will likely continue to grow as technological advances lead to enhanced portability and af... The application of bedside ultrasonography in routine clinical practice has dramatically evolved over the last few decades and will likely continue to grow as technological advances lead to enhanced portability and affordability of the equipment. Despite mounting interest, most nephrology fellowship training programs do not offer formal training in renal ultrasonography and there is inertia among practicing nephrologists to adopt this skill as a practice-changing advancement. Lack of familiarity with the topic is considered a key reason for this inertia. Understanding of basic ultrasound physics, instrumentation, principles of optimal image acquisition and interpretation is critical for enhanced efficiency and patient safety while using this tool. Herein, we provide a brief overview of the basic principles of diagnostic renal ultrasonography as well as introduction to common sonographic pathologies encountered in day-to-day nephrology practice with illustrative images. 展开更多
关键词 Ultrasound Kidney CYST HYDRONEPHROSIS STONE Point of care
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