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Responses of Soil Microbial Community Structure and Activity to Incorporation of Straws and Straw Biochars and Their Effects on Soil Respiration and Soil Organic Carbon Turnover
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作者 TIAN Xiaoping WANG Lei +3 位作者 HOU Yahong WANG Han TSANG Yiu Fai WU Jihua 《土壤圈:英文版》 SCIE CAS CSCD 2019年第4期492-503,共12页
Like straw, biochar incorporation can influence soil microorganisms and enzyme activities and soil carbon(C) responses;however,few studies have compared the various effects of straw and biochar and the underlying mech... Like straw, biochar incorporation can influence soil microorganisms and enzyme activities and soil carbon(C) responses;however,few studies have compared the various effects of straw and biochar and the underlying mechanisms. An experiment was performed to study the changes in soil respiration(SR) and soil organic C(SOC) fluxes in response to the incorporation of three kinds of straw(reed, smooth cordgrass, and rice) and their pyrolyzed products(biochars) at Chongming Island, China. In addition, the microbial activity and community structure of some amended soils were also analyzed to clarify the mechanisms of these responses. The results showed that all biochar incorporation(BC) induced lower SR than the corresponding unpyrolyzed straw incorporation(ST), and the average SR in the soils following BC and ST during the experimental periods was 21.69 and 65.32 μmol CO2 m^-2s^-1, respectively.Furthermore, the average SOC content was 16.97 g kg-1 following BC, which was higher than that(13.71 g kg-1) following ST,indicating that compared to ST, BC was a low-C strategy, even after accounting for the C loss during biochar production. Among the BC treatments, reed-BC induced the lowest SR(17.04 μmol CO2 m^-2s^-1), whereas smooth cordgrass-BC induced the highest SR(27.02 μmol CO2 m^-2s^-1). Furthermore, in contrast with ST, BC significantly increased the abundance of some bacteria with poorer mineralization or better humification ability, which led to lower SR. The lower easily oxidizable C(EOC) and higher total C contents of biochars induced lower SR and higher SOC in the soil following BC compared to that following ST. Among the BC treatments,the higher total nitrogen content of rice biochar led to significantly higher soil microbial biomass, and the lower EOC content of reed biochar led to lower soil microbial activity and SR. 展开更多
关键词 easily oxidizable CARBON SOIL fertility SOIL MICROBIAL abundance SOIL MICROBIAL biomass SOIL MICROBIAL diversity SOIL organic CARBON decomposition
Bar-headed goose into bird and chicks cloaca microbial of contrast analysis 预览
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作者 Xiaotian Zheng 《探索-植物与动物科学》 2019年第1期1-6,共6页
Intestinal Microbial by maintain steady-state, auxiliary digestion and promote immune system development and style maintenance host of health state. Intestinal micro-Biological itself by the host of gene, diet, age an... Intestinal Microbial by maintain steady-state, auxiliary digestion and promote immune system development and style maintenance host of health state. Intestinal micro-Biological itself by the host of gene, diet, age and environment and factors of influence. However intestinal microbial of change and Host Age Between the relationship still have many unknown. This study respectively collection bar-headed goose (Anser indicus)2Only into bird and3Only chicks cloaca Samples, Extraction intestinal microbial totalDNAThe16 S rRNAHigh Flux sequencing of Methods Analysis and compare the two age stage birds intestinal microbial of Flora structure and composition difference. Study found bar-headed goose chicks cloaca microbial belongs9A door content highest of before5A door respectively Is shuttle of door (48.29%), Thick-walled bacteria door (22.21%), Deformation of the door (22.07%), Actinomycetes door (5.02%) And soft wall bacteria door (1.93%). Into bird cloaca microbial belongs17A door most of in turn is the deformation of the door (64.69%), Thick-walled bacteria door (23.92%), Blue bacteria (8.48%), Actinomycetes door (1.43%) And shuttle of door (0.56%). In of the genus level bar-headed goose chicks cloaca microbial belongs18A of made bird containing24A. Into bird cloaca microbialαDiversity was significantly higher than that chicks (P<0.05Welch'sT-Test). 186A operation classification unit (OTU) Belongs to into bird and chicks there are and other640AOTUAnd90AOTUThe respectively belongs to into bird and chicks. Chicks in67.39%OfOtusIs into bird of some. Based onOTUThe clustering results and age group consensus. This results to know birds intestinal microbial and Host age change between the relationship have certain of reference value. 展开更多
关键词 Bar-headed GOOSE cloaca MICROBIAL MICROBIAL group High Flux SEQUENCING age
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微生物驱数值模拟研究进展 预览
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作者 王天源 修建龙 +3 位作者 崔庆锋 黄立信 马原栋 俞理 《中南大学学报:自然科学版》 EI CAS CSCD 北大核心 2019年第6期1474-1484,共11页
基于微生物驱采油机理,从数学模型和软件应用2个方面介绍和总结微生物驱数值模拟研究现状。由于经典微生物驱油模型(Islam模型、Zhang模型和Chang模型)不包含代谢产物组分,分别从产物主要以生物表面活性剂、生物聚合物以及其他产物模型... 基于微生物驱采油机理,从数学模型和软件应用2个方面介绍和总结微生物驱数值模拟研究现状。由于经典微生物驱油模型(Islam模型、Zhang模型和Chang模型)不包含代谢产物组分,分别从产物主要以生物表面活性剂、生物聚合物以及其他产物模型来阐述现存模型的优缺点;主要从MTS,UTCHEM,CMG-STARS,ECLIPSE和MRST这5个方面总结微生物驱数值模拟的软件应用。当前微生物驱数学模型无法准确体现实际微生物驱油过程,均存在些许不足。因此,今后研究的重点和趋势为:1)建立完善的多孔介质空间模型来准确描述多孔介质内部孔隙结构;2)系统地研究微生物的形状尺寸对微生物运移和驱油的影响;3)建立多因素、多组分耦合影响下的微生物驱数学模型。 展开更多
关键词 微生物 微生物提高采收率 数学模型 软件应用
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Sulfide mineral dissolution microbes: Community structure and function in industrial bioleaching heaps 预览
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作者 Yan Jia Qiaoyi Tan +3 位作者 Heyun Sun Yupeng Zhang Hongshan Gao Renman Ruan 《绿色能源与环境:英文版》 CSCD 2019年第1期29-37,共9页
Heap bioleaching is one of the most clean and economical processes for recovery of low-grade and complex ores, because the sulfide minerals are natural habitats for acidophiles capable of iron-and sulfur-oxidation. Th... Heap bioleaching is one of the most clean and economical processes for recovery of low-grade and complex ores, because the sulfide minerals are natural habitats for acidophiles capable of iron-and sulfur-oxidation. The most exciting advances in heap bioleaching are occurring in the field of microbiology, especially with the development of advanced molecular biology approaches. These chemolithotrophic microorganisms living in the acid mine environment fix N2 and CO2 and obtain energy for growth from soluble ferrous iron and reduced inorganic sulfur compounds during oxidation of sulfide minerals. The ferric iron as oxidant and sulfuric acid are a result of microbial activity and provide favorable conditions for the dissolution of sulfide minerals. Various microbial consortia were applied successfully in commercial bioleaching heaps around the world, and microbial community and activity were adapted related to the local climatic conditions, ore characteristics and engineering configuration. This review focuses on diversity of bioleaching microbes, their role in heap bioleaching processes, their community structure and function in industrial heaps and the relation to the ore characteristics and the engineering configuration, to give implications for optimizing leaching efficiency of heap bioleaching. 展开更多
关键词 ACIDOPHILES Engineering configuration HEAP BIOLEACHING MICROBIAL activity MICROBIAL COMMUNITY
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新生儿呼吸道感染产超广谱β-内酰胺酶菌的菌群分布及耐药性分析 预览
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作者 贺潇 申燕 +2 位作者 钟文 谢茜 李秋红 《国际检验医学杂志》 CAS 2019年第10期1205-1208,1212共5页
目的探讨新生儿呼吸道产超广谱β-内酰胺酶(ESBLs)菌的菌群分布及其耐药情况,指导临床合理用药。方法收集2013年1月至2018年9月分离自该院新生儿科患儿呼吸道的产ESBLs菌和非产ESBLs菌,采用肉汤法进行药敏试验。结果新生儿呼吸道感染产E... 目的探讨新生儿呼吸道产超广谱β-内酰胺酶(ESBLs)菌的菌群分布及其耐药情况,指导临床合理用药。方法收集2013年1月至2018年9月分离自该院新生儿科患儿呼吸道的产ESBLs菌和非产ESBLs菌,采用肉汤法进行药敏试验。结果新生儿呼吸道感染产ESBLs菌中肺炎克雷伯菌占72.73%,大肠埃希菌占27.27%,二者的ESBLs检出率分别为48.24%和28.12%。产ESBLs菌对部分抗菌药物耐药率明显高于非产ESBLs株,差异有统计学意义(P<0.05)。对添加β-内酰胺酶抑制剂而言,产ESBLs肺炎克雷伯菌对阿莫西林/克拉维酸、氨苄西林/舒巴坦和哌拉西林/他唑巴坦的耐药率分别为29.17%、90.63%和57.29%;而产ESBLs大肠埃希菌的耐药率分别为8.33%、38.89%和2.78%。产ESBLs肺炎克雷伯菌和大肠埃希菌的多重耐药率分别为97.92%和88.89%。结论新生儿呼吸道感染中产ESBLs菌检出率较高,且新生儿呼吸道产ESBLs菌的多重耐药率高于成人。体外抗菌试验结果显示对导致新生儿呼吸道感染的产ESBLs菌,不同含β-内酰胺酶抑制剂的复合β-内酰胺类抗菌药物其耐药率有差别,将对指导临床合理用药具有积极的意义。 展开更多
关键词 Β内酰胺酶类 婴儿 新生 呼吸道感染 抗药性 微生物 微生物敏感性试验
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Root Exudation of Organic Acids of Herbaceous Pioneer Plants and Their Growth in Sterile and Non-Sterile Nutrient-Poor,Sandy Soils from Post-Mining Sites
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作者 Katja BOLDT-BURISCH Bernd Uwe SCHNEIDER +1 位作者 M.Anne NAETH Reinhard F.HUTTL 《土壤圈:英文版》 SCIE CAS CSCD 2019年第1期34-44,共11页
Nutrient-poor, sandy soils form the prevailing substrate at post-mining sites of the Lusatian region(Brandenburg, Germany) and present a challenge for vegetation development. We studied the organic acid quantity and c... Nutrient-poor, sandy soils form the prevailing substrate at post-mining sites of the Lusatian region(Brandenburg, Germany) and present a challenge for vegetation development. We studied the organic acid quantity and composition of three commonly occurring pioneer plant species, the legumes Lotus corniculatus L. and Trifolium arvense L. and the grass Calamagrostis epigeios(L.) Roth, to determine if plant growth and exudation differed with(non-sterilized soil) and without(sterilized soil) an indigenous soil microbial community. We investigated whether organic acids were found in the rhizosphere and surrounding soil and whether this influenced nutrient mobilization. This study consists of linked field investigations and a greenhouse experiment. Plants were grown in the greenhouse in either sterilized or non-sterilized sandy soil from a reclamation site in the Lusatian mining landscape(Welzow Su¨d, East Germany). After seven months, the plant biomass, root morphology, organic acids, and water-soluble nutrients and root colonization with arbuscular mycorrhizal fungi(AMF) and dark septate endophytes(DSE) were analyzed. Roots of all three plants in the field and greenhouse experiments were highly colonized with AMF. Calamagrostis epigeios and T. arvense had a significantly higher colonization frequency with DSE than L. corniculatus. The quantity and composition of organic acids strongly differed among plant species, with the highest number of organic acids found for L. corniculatus and lowest for C. epigeios. The quantity of organic acids was greatly reduced in all plants under sterilized soil conditions. However, the composition of organic acids and plant growth in sterilized soil were reduced for both legumes, but not for C. epigeios, which had a higher biomass under sterilized conditions. Changes in nutrient concentrations in the field rhizosphere soil relative to those in the control were measurable after seven months. While the spectrum of organic acids and the growth of legumes seemed to be dependent on a highly 展开更多
关键词 Calamagrostis epigeios indigenous soil MICROBIAL community Lotus corniculatus MICROBIAL COLONIZATION mining landscape NUTRIENT mobilization root SYMBIOTIC partner TRIFOLIUM arvense
高原地区慢性化脓性中耳炎及中耳胆脂瘤的病原菌及药敏分析
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作者 吕芸 何健 +2 位作者 卫旭东 张邵婕 姚玉荣 《中国耳鼻咽喉头颈外科》 CSCD 2019年第1期21-24,共4页
目的 通过对高原地区中耳炎患者患耳分泌物病原微生物分布及主要致病菌的药物敏感性分析,指导临床合理用药。方法 收集2016年12月~2018年1月就诊于甘肃省人民医院耳鼻咽喉头颈外科,218例(220耳)甘肃周边各地县区(包括甘南地区)居住十年... 目的 通过对高原地区中耳炎患者患耳分泌物病原微生物分布及主要致病菌的药物敏感性分析,指导临床合理用药。方法 收集2016年12月~2018年1月就诊于甘肃省人民医院耳鼻咽喉头颈外科,218例(220耳)甘肃周边各地县区(包括甘南地区)居住十年以上患者,化脓耳分泌物,进行病原菌分离鉴定及药物敏感性试验。结果 (1)病原菌检出率69.1%,其中细菌感染125例,真菌感染8例。(2)慢性化脓性中耳炎革兰阳性菌检出率较高(68.8%),金黄色葡萄球菌最高。中耳胆脂瘤革兰阴性菌比例较高(30.2%),铜绿假单胞菌最常见。(3)常见致病菌对抗菌药物的敏感性因菌种而异。结论 致病菌主要以金黄色葡萄球菌、铜绿假单胞菌为主。常见致病菌对青霉素、左氧氟沙星等临床常用药物耐药性较高,故条件允许时应行细菌培养,指导合理用药。 展开更多
关键词 中耳炎 化脓性 胆脂瘤 中耳 集落计数 微生物 微生物敏感性试验
Comparative Study of Electrochemical Performance and Microbial Flora in Microbial Fuel Cells by Using Three Kinds of Substrates
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作者 ZHAO Yu FAN Lei +3 位作者 YANG Donghua DONG Zhishuai WANG Yuxue AN Xia 《高等学校化学研究:英文版》 SCIE CAS CSCD 2019年第2期292-298,共7页
This work aimed to investigate the distinct electrochemical performance and microbial flora of microbial fuel cells(MFCs)in relation to different single hazardous fed fuels.Three replicate MFCs were inoculated with th... This work aimed to investigate the distinct electrochemical performance and microbial flora of microbial fuel cells(MFCs)in relation to different single hazardous fed fuels.Three replicate MFCs were inoculated with the same microbial consortium from a coking wastewater treatment plants wherein ammonium chloride(ammoniiim chlo-ride-fed MFC,N-MFC),phenol(phenol-fed MFC,P-MFC)and potassium sulphide(potassium sulphide-fed MFC,S-MFC)were the sole substrates and main components of real coking wastewater.With initial concentrations of am-monium chloride,phenol and potassium sulphide of 0.75,0.60 and 0.55 g/L,the removal efficiencies reached 95.6%,90.6%and 99.9%,respectively,whereas the peak output power densities totalled 697,324 and 1215 mW/m^2.Micro-bial community analysis showed that the respective addition of substrate substantially altered the microbial community structure of anode biofllm,resulting in changes in relative abundance and emergence of new strains and further affecting the electrochemical properties of MFCs.The chemical oxygen demand(COD)removal efficiency of real coking wastewater,in which,the inoculum was the combined biomass from the three MFCs,reached 82.3%. 展开更多
关键词 COMPARISON of MICROBIAL FLORA COMPARISON of electrochemical performance SOLE substrate MICROBIAL fUel cell(MFC)
Based on MBR Microbial Community Structure Analysis of shortcut nitrification initiation in different kinds of mud 预览
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作者 Huang Dianxia Li Ziming 《探索-环境科学与技术》 2019年第1期1-5,共5页
For clear membrane bioreactor (MBR)Microbial Community structure changes before and after the short-cut Nitrification with different sludge inoculation.MBRNitrifying sludge (R1.),Anaerobic nitrification sludge (R2.)An... For clear membrane bioreactor (MBR)Microbial Community structure changes before and after the short-cut Nitrification with different sludge inoculation.MBRNitrifying sludge (R1.),Anaerobic nitrification sludge (R2.)And 1.:1.Mixed inoculation of anaerobic and Denitrifying sludge (R3.)Sludge source for achieving rapid short-cut nitrification.The results show that the combination of Intermittent Aeration and reduced hydraulic retention time (HRT),R1.R2.With R3.Reactor time-consuming 46 d,8 d And 30 d The shortcut nitrification was successfully launched,R2.Shortest startup cycle of Reactor.During the stable operation period,R1.R2.And R3.The average nitrite accumulation rate was 92%,93% And 94%,R3.The reactor showed more stable short-cut Nitrification performance..Ace,Chao,Shannon And Simpson Index results show that after stable operation,R1.And R2.The microbial abundance and diversity of the reactor were significantly lower than that of the inoculated sludge,R3.The abundance of reactor species decreased slightly while the diversity level changed little..After the shortcut nitrification was successfully started,3.The main bacteria in the reactor were Proteus phyla (Proteobacteria)And bacteriocin (Bacteroidetes)And the abundance of the Main Nitrogen Removal Function bacteria is higher than that of the inoculated sludge..Beta-Proteus3.The dominant bacteria in the short-cut Nitrification system of each reactor accounted59. 6%,63. 6%And69. 3%.R1.R2.AndR3.Reactor in the advantage bacteria of are nitrosation single (Nitrosomonas)of proportion respectively up 12. 8%,Natural 20. 2% And 19. 7%.Compared R1 Reactor,R2 And R3 Reactor inoculation sludge in there is a certain proportion of nitrification bacteria more conducive to system short-range nitrification of implementation. 展开更多
关键词 SHORT-RANGE NITRIFICATION Membrane Biological Reactor inoculation sludge start MICROBIAL community structure
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Safety evaluation of microbial pesticide (HaNPV) based on PCR method
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作者 Miao Zhao Shufei Li +3 位作者 Qinghong Zhou Dianming Zhou Ning He Zhiyong Qian 《化学科学与工程前沿:英文版》 EI CAS CSCD 2019年第2期377-384,共8页
Microbial pesticides can prevent and control diseases and pests of crops, and has become one of the important measures to ensure food and environmental safety. However, the potential harm of microbial pesticides to hu... Microbial pesticides can prevent and control diseases and pests of crops, and has become one of the important measures to ensure food and environmental safety. However, the potential harm of microbial pesticides to humans and animals is a serious concern at home and abroad. In this paper, we have investigated the infectivity and pathogenicity of a representative of viral microbial pesticides, helicoverpa armigera nuclear polyhedrosis virus (HaNPV), by specific and highly sensitive polymerase chain reaction technology. The results show that HaNPV can be gradually cleared in a short time after getting into blood of experimental rats, and does not infect other tissues or organs of animals;also indicate that the test subjects are not infectious to experimental rats after intravenous injection of HaNPV. Our method has good specificity and repeatability, and could provide an important reference for establishment of infectivity and pathogenicity detection methods for viral microbial pesticides in future. 展开更多
关键词 MICROBIAL PESTICIDES HANPV acute INTRAVENOUS INJECTION infectivity PATHOGENICITY
Designing an in situ remediation strategy for polluted surface water bodies through the specific regulation of microbial community
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作者 Boran Wu Xiaoli Chai +1 位作者 Youcai Zhao Xiaohu Dai 《中国环境科学与工程前沿:英文版》 SCIE EI CAS CSCD 2019年第1期21-26,共6页
This paper proposes an in situ restoration strategy, ecosystem activation system (EAS), for selfpurification abilities of polluted water ecosystems through the specific regulation of microbial community. EAS utilizes ... This paper proposes an in situ restoration strategy, ecosystem activation system (EAS), for selfpurification abilities of polluted water ecosystems through the specific regulation of microbial community. EAS utilizes polyhydroxyalkanoates (PHA) to modify indigenous microbial communities of polluted water bodies. Microorganisms preferentially utilizing PHA as the carbon source for their enhanced growth are defined as specific, and those with no special selectivity to PHA and raw-water dissolved organic matter are defined as non-specific and can be phased out during EAS operation. As a result, the microbial community can be regulated to the specific structure, which is beneficial for the water quality improvement. The developed model described the above principle with accounting for the growth kinetics of specific microorganisms, competitive inhabitation of specific microorganisms on non-specific microorganisms, and coupled EAS-induced self-purification of polluted water bodies. The conceptual model is believed to be a primary step toward quantitative design, operation, and optimization of EAS. 展开更多
关键词 REMEDIATION Surface water POLYHYDROXYALKANOATES MICROBIAL PROLIFERATION
Microbial Community Diversity and Vertical Distribution in a Columnar Sediment of Maluku Strait 预览
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作者 Yan Wang Fuchao Li +3 位作者 Jin Zhao Huaxin Chen Peng Jiang Xuexi Tang 《大气科学研究(英文)》 2019年第2期51-58,共8页
The sediment samples were collected from Maluku Strait at a depth of 1250 m, which is influenced by Mindanao Current and Indonesian Throughflow. Based on 16S rRNA clone libraries, the community structure and vertical ... The sediment samples were collected from Maluku Strait at a depth of 1250 m, which is influenced by Mindanao Current and Indonesian Throughflow. Based on 16S rRNA clone libraries, the community structure and vertical distribution of archaea and bacteria were studied in a columnar sediment of 2m in length. From the surface sediment, 16S sequences were derived from fourteen bacterial phyla (Gammaproteobacteria, Planctomycetes, Alphaproteobacteria, Deltproteobacteria were dominant), but were limited to two groups of archaea: Crenarchaeota (99%) and Euryarchaeota (1%). Besides, 90% of the archaea clones were ammonia oxidation-related which indicated that the ammonia-oxidizing archaea might make a significant contribution to the chemosynthesis in the surface sediment. Contrastively in the bottom sediment, six bacterial phylogenetic groups were obtained (Gammaproteobacteria and Firmicutes were absolutely dominant), however no archaea 16S rRNA was detected. The microbial diversity of surface sediment was much higher than the bottom and seven unique bacterial phyla were obtained from two sediment respectively. The geochemical elements analysis revealed that the content of C, TOC and S in the surface sediment was much higher than the bottom, but the content of P is contrary. The microbial communities might be in response to the geochemical substance transfer and deposit influenced by the ocean current and it deserves further study compared with the other sediment samples in this area. 展开更多
关键词 Maluku STRAIT SEDIMENT 16S RRNA MICROBIAL COMMUNITY Spatial distribution
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Upgrading VFAs bioproduction from waste activated sludge via co-fermentation with soy sauce residue
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作者 Yanqing Duan Aijuan Zhou +4 位作者 Kaili Wen Zhihong Liu Wenzong Liu Aijie Wang Xiuping Yue 《中国环境科学与工程前沿:英文版》 SCIE EI CAS CSCD 2019年第1期53-62,共10页
Conditioning of extra carbon sources has been widely reported to facilitate fermentation of waste activated sludge (WAS). Soy sauce residue (SSR) was a relatively untapped carbon source for sludge conditioning. This b... Conditioning of extra carbon sources has been widely reported to facilitate fermentation of waste activated sludge (WAS). Soy sauce residue (SSR) was a relatively untapped carbon source for sludge conditioning. This batch study aimed to evaluate the possible implementation of SSR for volatile fatty acids (VFAs) production from WAS. To upgrade the bioavailability of feedstock, three typical pretreatment methods were conducted, i.e., ammonium hydroxide (AH), sulfuric acids (SA) and thermal assisted alkaline (TA). AH pretreated test (AH-PT) outperformed due to a relatively strong structure decomposition of cellulosic materials as revealed by infrared spectroscopic analysis and crystal index. As a result, performed a high hydrolysis rate of 4449 mg COD/d, 1.12-1.23-fold higher than that in TA and SA pretreated tests (TA-PT and SA-PT), and 7.8-fold higher than that in the Control test. Meanwhile, a volatile fatty acids (VFAs) contribution of 401.2 mg COD/g SSR L and a maximum acidification rate of 3.59 d was recorded, with a high sum proportion of mall molecular acetic and propionic 82.2%, 11%-70% increase over the other three tests. Besides, speciation process characterized with functional genus differentiation was identified by microbial diversity and distribution investigation and canonical correspondence analysis (CCA). Finally, a potential market value of 0.49-0.65 Billion €/year was preliminary estimated, showing promise of resource recovery from both WAS and SSR instead of extensive disposal. 展开更多
关键词 Waste activated SLUDGE (WAS) SOY SAUCE residue (SSR) SLUDGE conditioning VOLATILE fatty acids (VFAs) Microbial diversity
Enhanced nitrate reduction in water by a combined bio-electrochemical system of microbial fuel cells and submerged aquatic plant Ceratophyllum demersum
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作者 Peng Xu Enrong Xiao +3 位作者 Junmei Wu Feng He Yi Zhang Zhenbin Wu 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第4期338-351,共14页
High nitrate(NO3-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO3-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerged aq... High nitrate(NO3-)loading in water bodies is a crucial factor inducing the eutrophication of lakes.We tried to enhance NO3-reduction in overlying water by coupling sediment microbial fuel cells(SMFCs)with submerged aquatic plant Ceratophyllum demersum.A comparative study was conducted by setting four treatments:open-circuit SMFC(Control),closed-circuit SMFC(SMFC-c),open-circuit SMFC with C.demersum(Plant),and closed-circuit SMFC with C.demersum(P-SMFC-c).The electrochemical parameters were documented to illustrate the bio-electrochemical characteristics of SMFC-c and P-SMFC-c.Removal pathways of NO3- in different treatments were studied by adding quantitative15NO3- to water column.The results showed that the cathodic reaction in SMFC-c was mainly catalyzed by aerobic organisms attached on the cathode,including algae,Pseudomonas,Bacillus,and Albidiferax.The oxygen secreted by plants significantly improved the power generation of SMFC-c.Both electrogenesis and plants enhanced the complete removal of NO3- from the sediment–water system.The complete removal rates of added15N increased by 17.6% and 10.2% for SMFC-c and plant,respectively,when compared with control at the end of experiment.The electrochemical/heterotrophic and aerobic denitrification on cathodes mainly drove the higher reduction of NO3- in SMFC-c and plant,respectively.The coexistence of electrogenesis and plants further increased the complete removal of NO3- with a rate of 23.1%.The heterotrophic and aerobic denitrifications were simultaneously promoted with a highest abundance of Flavobacterium,Bacillus,Geobacter,Pseudomonas,Rhodobacter,and Arenimonas on the cathode. 展开更多
关键词 SEDIMENT MICROBIAL fuel cells(SMFCs) Ceratophyllum demersum Power generation Nitrate(NO3-) REMOVAL Stable N ISOTOPIC REMOVAL mechanisms
Urban recreational water – potential breeding ground for antibiotic resistant bacteria?
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作者 Jing Yang Xu Connie Le +1 位作者 Baowei Chen Hongquan Zhang 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第7期1-3,共3页
The emergence of multidrug-resistant bacteria, or'superbugs,' has become an urgent and worldwide concern.Antibiotic-resistant bacteria (ARB) cause significant morbidity and mortality (O’Neill, 2016). It has b... The emergence of multidrug-resistant bacteria, or'superbugs,' has become an urgent and worldwide concern.Antibiotic-resistant bacteria (ARB) cause significant morbidity and mortality (O’Neill, 2016). It has been estimated that ARB cause at least 23,000 deaths annually in the United States alone and hundreds of thousands of deaths in developing countries (Bougnom and Piddock, 2017). 展开更多
关键词 Antibiotic RESISTANCE genes (ARGs) MULTIDRUG RESISTANCE in bacteria MICROBIAL PATHOGENS LAKE water and sediment
Hydrogen Production Using “Direct-Starting” Biocathode Microbial Electrolysis Cell and the Analysis of Microbial Communities
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作者 Hong-Yan Dai Hui-Min Yang +2 位作者 Xian Liu Xiu-Li Song Zhen-Hai Liang 《金属学报:英文版》 SCIE EI CAS CSCD 2019年第3期297-304,共8页
In this study, a ‘‘direct-starting’’ procedure was used to activate a single-chamber biocathode microbial electrolysis cell(MEC) and the development of a biocathode was studied through output current curves and cy... In this study, a ‘‘direct-starting’’ procedure was used to activate a single-chamber biocathode microbial electrolysis cell(MEC) and the development of a biocathode was studied through output current curves and cyclic voltammograms. It only took 163 h for a successful start-up, and a current density of 14.75 A/m2 was obtained. In the formal hydrogen-production stage, it was found that the biocathode MEC was comparable with the Pt/C cathode MEC in terms of current density and energy efficiency, and the hydrogen recovery, cathodic hydrogen recovery, and hydrogen production rate of the biocathode MEC were 71.22% ± 8.98%, 79.42% ± 5.94%, and 0.428 ± 0.054 m3 H2/m3 days, respectively, which were slightly higher than those obtained with the Pt/C cathode MEC. Besides, under the effect of applied voltage, the microbial populations in the anodophilic biofilm of MEC(MECan) and the cathodophilic biofilm of MEC(MECca) were less diverse than those of the original aerobic activated sludge(AAS) and the anodophilic biofilm of MEC(MECan). Furthermore, the microbial community structures evidently differed between MECan/MECca and AAS/MFC. 展开更多
关键词 “Direct-starting” procedure BIOCATHODE HYDROGEN production MICROBIAL COMMUNITY structures
Identification of microbial contaminants in sinus rinse squeeze bottles used by allergic rhinitis patients
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作者 Shaun A.Nguyen Marc P.Camilon Rodney J.Schlosser 《世界耳鼻咽喉头颈外科杂志(英文)》 2019年第1期26-29,共4页
Objective:To identify whether irrigation devices become contaminated when used by patients with allergic rhinitis (AR).Methods:Ten AR patients with no clinical or endoscopic evidence of active sinonasal infection were... Objective:To identify whether irrigation devices become contaminated when used by patients with allergic rhinitis (AR).Methods:Ten AR patients with no clinical or endoscopic evidence of active sinonasal infection were given a sinus rinse system and instructed on its proper use,cleaning,and storage.Two squeeze bottles (bottle A and bottle B) were given to each patient for twice-a-day rinsing.Bottle A was used in the morning and analyzed after four weeks.Bottle B was used in the evening and analyzed after 8 weeks of use.Microbial contaminants were cultured from the nose pieces and the inner surface of the bottles obtained from patients.Results:Seventeen sinus rinse devices (17/20) from all individuals in this study grew bacteria commonly in the nozzles.Twenty-four bacterial isolates consisting of 14 different species were cultured and identified with most common organisms being bacilli and staphylococcus.In addition,no correlation was apparent between the length of bottle use and the degree of contamination (r =0.13,p =0.76).During the study period,no patient developed acute sinus infections.Conclusion:Microbial contamination of the sinus rinse system occurs commonly,even in uninfected AR patients;however no evidence exists linking this to clinically relevant sinus infections. 展开更多
关键词 Allergic RHINITIS Microbial contaminants SINUS irrigation SINUS RINSE SQUEEZE BOTTLES
Microbial diversity in Huguangyan Maar Lake of China revealed by high-throughput sequencing 预览
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作者 HOU Qinghua FANG Zhou +1 位作者 ZHU Qingmei DONG Hongpo 《海洋湖沼学报(英文)》 SCIE CAS CSCD 2019年第4期1245-1257,共13页
Huguangyan Maar Lake is a typical maar lake in the southeast of China. It is well preserved and not disturbed by anthropogenic activities. In this study, microbial community structures in sediment and water samples fr... Huguangyan Maar Lake is a typical maar lake in the southeast of China. It is well preserved and not disturbed by anthropogenic activities. In this study, microbial community structures in sediment and water samples from Huguangyan Maar Lake were investigated using a high-throughput sequencing method. We found significant differences between the microbial community compositions of the water and the sediment. The sediment samples contained more diverse Bacteria and Archaea than did the water samples. Actinobacteria, Betaproteobacteria, Cyanobacteria, and Deltaproteobacteria predominated in the water samples while Deltaproteobacteria, Anaerolineae, Nitrospira, and Dehalococcoidia were the major bacterial groups in the sediment. As for Archaea, Woesearchaeota (DHVEG-6), unclassified Archaea, and Deep Sea Euryarchaeotic Group were detected at higher abundances in the water, whereas the Miscellaneous Crenarchaeotic Group, Thermoplasmata, and Methanomicrobia were significantly more abundant in the sediment. Interactions between Bacteria and Archaea were common in both the water column and the sediment. The concentrations of major nutrients (NO^3-, PO4^3-, SiO3^2- and NH4^+) shaped the microbial population structures in the water. At the higher phylogenetic levels including phylum and class, many of the dominant groups were those that were also abundant in other lakes;however, novel microbial populations (unclassified) were often seen at the lower phylogenetic levels. Our study lays a foundation for examining microbial biogeochemical cycling in sequestered lakes or reservoirs. 展开更多
关键词 Huguangyan MAAR LAKE HIGH-THROUGHPUT SEQUENCING MICROBIAL diversity
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两种配方废弃菌棒堆肥中微生物多样性研究 预览
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作者 代晓航 魏超 《农业机械学报》 EI CAS CSCD 北大核心 2019年第7期275-280,321共7页
以废弃菌棒为主要原料,添加牛粪和草炭,进行堆肥发酵,取腐熟完成后的2种肥料样品DNA,采用16SrDNA V3+V4区和ITS2高通量测序方法,研究不同配方粗制肥料中微生物多样性和群落结构的差异。结果表明,“牛粪+菌棒”制得的有机肥生物多样性高... 以废弃菌棒为主要原料,添加牛粪和草炭,进行堆肥发酵,取腐熟完成后的2种肥料样品DNA,采用16SrDNA V3+V4区和ITS2高通量测序方法,研究不同配方粗制肥料中微生物多样性和群落结构的差异。结果表明,“牛粪+菌棒”制得的有机肥生物多样性高于“草炭+菌棒”,且真菌差异大于细菌;“牛粪+菌棒”有机肥相对“草炭+菌棒”有机肥具有10个优势细菌属和6个真菌属,“草炭+菌棒”有机肥相对“牛粪+菌棒”有机肥具有14个优势细菌属和4个真菌属。两种配方堆肥制得的有机肥微生物群落结构无显著性差异。研究发现了多种具有农业价值的功能菌,可为功能性微生物的开发利用提供科学依据。此外,也发现一些潜在危害因子,如黄曲霉和杂色曲霉亚种在“牛粪+菌棒”有机肥中丰度较高,产生的黄曲霉毒素、杂曲霉毒素易造成农作物污染。 展开更多
关键词 菌棒 有机肥 微生物 高通量测序
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Agricultural Risk Factors Influence Microbial Ecology in Honghu Lake
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作者 Maozhen Han Melissa Dsouza +9 位作者 Chunyu Zhou Hongjun Li Junqian Zhang Chaoyun Chen Qi Yao Chaofang Zhong Hao Zhou Jack A Gilbert Zhi Wang Kang Ning 《基因组蛋白质组与生物信息学报:英文版》 CAS CSCD 2019年第1期76-90,共15页
Agricultural activities,including stock-farming,planting industry,and fish aquaculture,can affect the physicochemical and biological characters of freshwater lakes.However,the effects of pollution producing by agricul... Agricultural activities,including stock-farming,planting industry,and fish aquaculture,can affect the physicochemical and biological characters of freshwater lakes.However,the effects of pollution producing by agricultural activities on microbial ecosystem of lakes remain unclear.Hence,in this work,we selected Honghu Lake as a typical lake that is influenced by agriculture activities.We collected water and sediment samples from 18 sites,which span a wide range of areas from impacted and less-impacted areas.We performed a geospatial analysis on the composition of microbial communities associated with physicochemical properties and antibiotic pollution of samples.The co-occurrence networks of water and sediment were also built and analyzed.Our results showed that the microbial communities of impacted and less-impacted samples of water were largely driven by the concentrations of TN,TP,NO3-N,and NO[-N,while those of sediment were affected by the concentrations of Sed-OM and Sed-TN.Antibiotics have also played important roles in shaping these microbial communities:the concentrations of oxytetracycline and tetracycline clearly reflected the variance in taxonomic diversity and predicted functional diversity between impacted and less-impacted sites in water and sediment samples,respectively.Furthermore,for samples from both water and sediment,large differences of network topology structures between impacted and less-impacted were also observed.Our results provide compelling evidence that the microbial community can be used as a sentinel of eutrophication and antibiotics pollution risk associated with agricultural activity;and that proper monitoring of this environment is vital to maintain a sustainable environment in Honghu Lake. 展开更多
关键词 FRESHWATER MICROBIAL COMMUNITIES Agriculture activities ANTIBIOTICS Human impact
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