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Relations between indoor and outdoor PM2.5 and constituent concentrations
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作者 Cong Liu Yinping Zhang 《中国环境科学与工程前沿:英文版》 SCIE EI CAS CSCD 2019年第1期1-20,共20页
Outdoor PM2.5 influences both the concentration and composition of indoor PM2.5. People spend over 80% of their time indoors. Therefore, to assess possible health effects of PM2.5 it is important to accurately charact... Outdoor PM2.5 influences both the concentration and composition of indoor PM2.5. People spend over 80% of their time indoors. Therefore, to assess possible health effects of PM2.5 it is important to accurately characterize indoor PM2.5 concentrations and composition. Controlling indoor PM2.5 concentration is presently more feasible and economic than decreasing outdoor PM2.5 concentration. This study reviews modeling and measurements that address relationships between indoor and outdoor PM2.5 and the corresponding constituent concentrations. The key factors in the models are indooroutdoor air exchange rate, particle penetration, and deposition. We compiled studies that report I/O ratios of PM7.5 and typical constituents (sulfate (SO4^2-), nitrate (NO3 ), ammonium (NH4^+), elemental carbon (EC), and organic carbon (OC), iron (Fe), copper (Cu), and manganese (Mn)). From these studies we conclude that: 1) sulfate might be a reasonable tracer of non-volatile species (EC, Fe, Cu, and Mn) and PM2.5 itself;2) particulate nitrate and ammonium generally desorb to gaseous HNO3 and NH3 when they enter indoors, unless, as seldom happens, they have strong indoor sources;3) indoor-originating semi-volatile organic compounds sorb on indoor PM2.5, thereby increasing the PM2.5 OC load. We suggest further studies on indoor-outdoor relationships of PM2.5 and constituents so as to help develop standards for healthy buildings. 展开更多
关键词 INDOOR air quality Exposure SVOC REACTIVE OXIDATIVE species OXIDATIVE potential Chemical transport model
Chronic Intracerebroventricular Infusion of Metformin Inhibits Salt-Sensitive Hypertension via Attenuation of Oxidative Stress and Neurohormonal Excitation in Rat Paraventricular Nucleus
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作者 Xiao-Jing Yu Ya-Nan Zhao +13 位作者 Yi-Kang Hou Hong-Bao Li Wen-Jie Xia Hong-Li Gao Kai-Li Liu Qing Su Hui-Yu Yang Bin Liang Wen-Sheng Chen Wei Cui Ying Li Guo-Qing Zhu Zhi-Ming Yang Yu-Ming Kang 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第1期57-66,共10页
Metformin(MET), an antidiabetic agent, also has antioxidative effects in metabolic-related hypertension.This study was designed to determine whether MET has anti-hypertensive effects in salt-sensitive hypertensive rat... Metformin(MET), an antidiabetic agent, also has antioxidative effects in metabolic-related hypertension.This study was designed to determine whether MET has anti-hypertensive effects in salt-sensitive hypertensive rats by inhibiting oxidative stress in the hypothalamic paraventricular nucleus(PVN). Salt-sensitive rats received a highsalt(HS) diet to induce hypertension, or a normal-salt(NS)diet as control. At the same time, they received intracerebroventricular(ICV) infusion of MET or vehicle for 6 weeks. We found that HS rats had higher oxidative stress levels and mean arterial pressure(MAP) than NS rats. ICV infusion of MET attenuated MAP and reduced plasma norepinephrine levels in HS rats. It also decreased reactive oxygen species and the expression of subunits of NAD(P)H oxidase, improved the superoxide dismutase activity,reduced components of the renin-angiotensin system, and altered neurotransmitters in the PVN. Our findings suggest that central MET administration lowers MAP in saltsensitive hypertension via attenuating oxidative stress,inhibiting the renin-angiotensin system, and restoring the balance between excitatory and inhibitory neurotransmitters in the PVN. 展开更多
关键词 Hypertension PARAVENTRICULAR NUCLEUS Sympathoexcitation METFORMIN OXIDATIVE stress
Overexpression of steroid receptor coactivators alleviates hyperglycemia-induced endothelial cell injury in rats through activating the PI3K/Akt pathway
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作者 Xiao-juan Quan Chun-lian Liang +2 位作者 Ming-zhu Sun Lin Zhang Xiu-li Li 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第5期648-657,共10页
Hyperglycemia is a major factor in vascular endothelial injury that finally leads to a cardiovascular event. Steroid receptor coactivators (SRCs) are a group of non-DNA binding proteins that induce structural changes ... Hyperglycemia is a major factor in vascular endothelial injury that finally leads to a cardiovascular event. Steroid receptor coactivators (SRCs) are a group of non-DNA binding proteins that induce structural changes in steroid receptors (nuclear receptors) critical for transcriptional activation. SRCs, namely, SRC-1, SRC-2, and SRC-3, are implicated in the regulation of vascular homeostasis. In this study we investigate the role of SRCs in hyperglycemia-induced endothelial injury. Aortic endothelial cells were prepared from normal and diabetic rats, respectively. Diabetic rats were prepared by injection of streptozotocin (50 mg/kg, i.p.). The expression levels of SRC-1 and SRC-3 were signi?cantly decreased in endothelial cells from the diabetic rats. Similar phenomenon was also observed in aortic endothelial cells from the normal rats treated with a high glucose (25 mM) for 4 h or 8 h. The expression levels of SRC-2 were little affected by hyperglycemia. Overexpression of SRC-1 and SRC-3 in high glucose-treated endothelial cells significantly increased the cell viability, suspended cell senescence, and inhibited cell apoptosis compared with the control cells. We further showed that overexpression of SRC-1 and SRC-3 markedly suppressed endothelial injury through restoring nitric oxide production, upregulating the expression of antioxidant enzymes (SOD, GPX, and CAT), and activating the PI3K/Akt pathway. The beneficial effects of SRC-1 and SRC-3 overexpression were blocked by treatment with the PI3K inhibitor LY294002 (10 mM) or with the Akt inhibitor MK-2206 (100 nM). In conclusion, hyperglycemia decreased SRC-1 and SRC-3 expression levels in rat aortic endothelial cells. SRC-1 and SRC-3 overexpression might protect against endothelial injury via inhibition of oxidative stress and activation of PI3K/Akt pathway. 展开更多
关键词 DIABETICS STREPTOZOTOCIN HYPERGLYCEMIA endothelial cells STEROID receptor coactivators CELL SENESCENCE CELL apoptosis oxidative stress PI3K/Akt pathway LY294002 MK-2206
Exposure to Prorocentrum minimum Induces Oxidative Stress and Apoptosis in the Ridgetail White Prawn, Exopalaemon carinicauda 预览
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作者 MU Cuimin GE Qianqian LI Jian 《中国海洋大学学报:英文版》 SCIE CAS CSCD 2019年第3期727-734,共8页
Prorocentrum minimum is a bloom-forming, planktonic mixotrophic dinoflagellate, and can cause stress in shrimp ponds. In this study, healthy Exopalaemon carinicauda were exposed to 5 × 10^4 cells mL^-1 P. minimum... Prorocentrum minimum is a bloom-forming, planktonic mixotrophic dinoflagellate, and can cause stress in shrimp ponds. In this study, healthy Exopalaemon carinicauda were exposed to 5 × 10^4 cells mL^-1 P. minimum for 72 hours to investigate the adverse effect of P. minimum on shrimps. Elevated superoxide dismutase(SOD) activity and malondialdehyde(MDA) content, reduced total antioxidant capacity(T-AOC) and catalase(CAT) activity, and regulatory glutathione peroxidase(GPX) activity were found in the hemolymph of E. carinicauda after exposure to P. minimum. In this study, P. minimum exposure induced oxidative stress and caused significant oxidative damage to E. carinicauda. P. minimum exposure increased the expression of HSP70 gene in the hemocyte, gills and hepatopancreas. Compared with the enhanced level of caspase-3 gene mR NA in the hemocyte and gills, the up-regulation of caspase-3 gene in the hepatopancreas was only observed from 3 to 6 h, and then the mRNA level of glutathione-S-transferase(GST) gene increased. These results indicated that GST might be involved in the shrimp hepatopancreas’ defense against P. minimum exposure. The present study demonstrates that exposure to P. minimum could induce oxidative stress and apoptosis in E. carinicauda. The SOD activity, HSP70 and GST(in the hepatopancreas) were evoked to protect cells from oxidative stress and apoptosis. This study will provide new insights into the toxic mechanism of P. minimum on shrimps. 展开更多
关键词 PROROCENTRUM minimum EXOPALAEMON carinicauda OXIDATIVE stress APOPTOSIS HSP70
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New neuroprotective perspectives in fighting oxidative stress and improving cellular energy metabolism by oleocanthal 预览
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作者 Cristina Angeloni Laura Giusti Silvana Hrelia 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1217-1218,共2页
Neurodegenerative diseases are a heterogeneous group of disorders characterized by a progressive dysfunction and death of neural cells which lead to compromised motor or cognitive function.Morphologically,the loss of ... Neurodegenerative diseases are a heterogeneous group of disorders characterized by a progressive dysfunction and death of neural cells which lead to compromised motor or cognitive function.Morphologically,the loss of neuron is associated with both gliosis and,frequently,with abnormal accumulation of extracellular and intracellular filamentous deposit in specific cell types.With a rapidly increasing aging population,these diseases are becoming a primary health problem(Albers and Beal,2000). 展开更多
关键词 NEW NEUROPROTECTIVE FIGHTING OXIDATIVE
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Up-Regulation of Trem2 Inhibits Hippocampal Neuronal Apoptosis and Alleviates Oxidative Stress in Epilepsy via the PI3K/Akt Pathway in Mice
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作者 Ai-Hua Liu Min Chu Yu-Ping Wang 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第3期471-485,共15页
Epilepsy is a chronic and severe neurological disorder that has negative effects on the autonomous activities of patients. Functionally, Trem2(triggering receptor expressed on myeloid cells-2) is an immunoglobulin rec... Epilepsy is a chronic and severe neurological disorder that has negative effects on the autonomous activities of patients. Functionally, Trem2(triggering receptor expressed on myeloid cells-2) is an immunoglobulin receptor that affects neurological and psychiatric genetic diseases. Based on this rationale, we aimed to assess the potential role of Trem2 integration with the PI3 K/Akt pathway in epilepsy. We used microarray-based gene expression profiling to identify epilepsy-related differentially-expressed genes. In a mouse hippocampal neuron model of epilepsy, neurons were treated with lowMg^2+ extracellular fluid, and the protein and mRNA expression of Trem2 were determined. Using a gain-offunction approach with Trem2, neuronal apoptosis and its related factors were assessed by flow cytometry, RT-qPCR,and Western blot analysis. In a pilocarpine-induced epileptic mouse model, the malondialdehyde(MDA) and8-hydroxy-20-deoxyguanosine(8-OHdG) content and superoxide dismutase(SOD) and glutathione-peroxidase(GSH-Px) activity in the hippocampus were determined,and the protein expression of Trem2 was measured. In addition, the regulatory effect of Trem2 on the PI3 K/Akt pathway was analyzed by inhibiting this pathway in both the cell and mouse models of epilepsy. Trem2 was found to occupy a core position and was correlated with epilepsy.Trem2 was decreased in the hippocampus of epileptic miceand epileptic hippocampal neurons. Of crucial importance,overexpression of Trem2 activated the PI3 K/Akt pathway to inhibit neuronal apoptosis. Moreover, activation of the PI3 K/Akt pathway through over-expression of Trem2 alleviated oxidative stress, as shown by the increased expression of SOD and GSH-Px and the decreased expression of MDA and 8-OHdG. The current study defines the potential role of Trem2 in inhibiting the development of epilepsy, indicating that Trem2 up-regulation alleviates hippocampal neuronal injury and oxidative stress, and inhibits neuronal apoptosis in epilepsy by activating the PI3 K/Akt pathway. 展开更多
关键词 Trem2 EPILEPSY NEURONAL apoptosis PI3K/Akt pathway OXIDATIVE stress
Selenium-catalyzed oxidative carbonylation of 1,2,3-thiadiazol-5-amine with amines to 1,2,3-thiadiazol-5-ylureas
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作者 Xiaopeng Zhang Shuxiang Dong +2 位作者 Qianqian Ding Xuesen Fan Guisheng Zhang 《中国化学快报:英文版》 SCIE CAS CSCD 2019年第2期375-378,共4页
A facile one-pot, economical approach to 1,2,3-thiadiazol-5-ylureas was developed via seleniumcatalyzed oxidative carbonylation of 1,2,3-thiadiazol-5-amine with a series of amines in one-pot manner in the presence of ... A facile one-pot, economical approach to 1,2,3-thiadiazol-5-ylureas was developed via seleniumcatalyzed oxidative carbonylation of 1,2,3-thiadiazol-5-amine with a series of amines in one-pot manner in the presence of CO and O2. This approach is featured with cheap and easily available raw materials,cheap and reusable catalyst selenium, one-pot procedure, high atomic economy, simple operations and no emission of corrosive wastes. 展开更多
关键词 SELENIUM Oxidative CARBONYLATION 1 2 3-Thiadiazol-5-amine 1 2 3-Thiadiazol-5-ylureas PHASE-TRANSFER CATALYSIS
Ginsenoside Rgl protects against ischemic/reperfusioninduced neuronal injury through miR-144/Nrf2/ARE pathway
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作者 Shi-feng Chu Zhao Zhang +10 位作者 Xin Zhou Wen-bin He Chen Chen Piao Luo Dan-dan Liu Qi-di Ai Hai-fan Gong Zhen-zhen Wang Hong-shuo Sun Zhong-ping Feng Nai-hong Chen 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第1期13-25,共13页
Ginsenoside Rg1 (Rg1),a saponin extracted from Panax ginseng,has been well documented to be effective against ischemic/ reperfusion (I/R)neuronal injury.However,the underlying mechanisms remain obscure.In the present ... Ginsenoside Rg1 (Rg1),a saponin extracted from Panax ginseng,has been well documented to be effective against ischemic/ reperfusion (I/R)neuronal injury.However,the underlying mechanisms remain obscure.In the present study,we investigated the roles of Nrf2 and miR-144 in the protective effects of Rgl against I/R-induced neuronal injury.In OGD/R-treated PC12 cells,Rgl (0.01-1 μmol/L)dose-dependently attenuated the cell injury accompanied by prolonging nuclear accumulation of Nrf2,enhancing the transcriptional activity of Nrf2,as well as promoting the expression of ARE-target genes.The activation of the Nrf2/ARE pathway by Rgl was independent of disassociation with Keapl,but resulted from post-translational regulations.Knockdown of Nrf2 abolished all the protective changes of Rgl in OG-D/R-treated PC12 cells.Furthermore,Rgl treatment significantly decreased the expression of miR-144,which downregulated Nrf2 production by targeting its 3'-untranlated region after OGD/R.Knockdown of Nrf2 had no effect on the expression of miR-144,suggesting that miR-144 was an upstream regulator of Nrf2.We revealed that there was a direct binding between Nrf2 and miR-144 in PC12 cells.Application of anti-miR-144 occluded the activation of the Nrf2/ ARE pathway by Rgl in OGD/R-treated PC12 cells.In tMCAO rats,administration of Rgl (20 mg/kg).significantly alleviated ischemic injury,and activated Nff2/ARE pathway.The protective effects of Rgl were abolished by injecting of AAV-HIF-miR-144-shRNA into the predicted ischemic penumbra.In conclusion,our results demonstrate that Rgl alleviates oxidative,stress after I/R through inhibiting miR-144 activity and subsequently promoting the Nrf2/ARE pathway at the post-translational-level. 展开更多
关键词 STROKE GINSENOSIDE Rgl ischemic/reperfusion oxidative stress Nrf2/ARE miR-144 PC12 cells tMCAO rats
Effects of apoptosis on liver aging 预览
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作者 Shao-Jie Hu Sha-Sha Jiang +8 位作者 Jin Zhang Dan Luo Bo Yu Liang-Yan Yang Hua-Hua Zhong Mei-Wen Yang Li-Yu Liu Fen-Fang Hong Shu-Long Yang 《世界临床病例杂志》 2019年第6期691-704,共14页
As an irreversible and perennial process, aging is accompanied by functional and morphological declines in organs. Generally, aging liver exhibits a decline in volume and hepatic blood flow. Even with a preeminent reg... As an irreversible and perennial process, aging is accompanied by functional and morphological declines in organs. Generally, aging liver exhibits a decline in volume and hepatic blood flow. Even with a preeminent regenerative capacity to restore its functions after liver cell loss, its biosynthesis and metabolism abilities decline, and these are difficult to restore to previous standards. Apoptosis is a programmed death process via intrinsic and extrinsic pathways, in which Bcl-2 family proteins and apoptosis-related genes, such as p21 and p53, are involved. Apoptosis inflicts both favorable and adverse influences on liver aging. Apoptosis eliminates transformed abnormal cells but promotes age-related liver diseases, such as nonalcoholic fatty liver disease, liver fibrosis, cirrhosis, and liver cancer. We summarize the roles of apoptosis in liver aging and age-related liver diseases. 展开更多
关键词 APOPTOSIS LIVER AGING OXIDATIVE stress Caloric RESTRICTION CIRRHOSIS
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Polycrystalline Phase WO3/g-C3N4 as a High Efficient Catalyst for Removal of DBT in Model Oil 预览
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作者 Li Xiuping Zhao Rongxiang Mao Chunfeng 《中国炼油与石油化工:英文版》 SCIE CAS 2019年第1期36-45,共10页
The polycrystalline phase WO3/g-C3N4 was synthesized under stirring using tungstenic acid(H2WO4) and graphitic carbon nitride(g-C3N4) as raw materials. The catalyst was characterized by X-ray diffraction(XRD), scannin... The polycrystalline phase WO3/g-C3N4 was synthesized under stirring using tungstenic acid(H2WO4) and graphitic carbon nitride(g-C3N4) as raw materials. The catalyst was characterized by X-ray diffraction(XRD), scanning electron microscopy(SEM),energy dispersive spectroscopy(EDS),the Fourier transform infrared spectroscopy(FT-IR),and the Brunauer-Emmett-Teller analysis(BET). The polycrystalline phase WO3/g-C3N4 was determined by XRD technique. The oxidative desulfurization process was investigated using WO3/g-C3N4 as the catalyst, 30% hydrogen peroxide(H202) as the oxidant, and 1-butyl-3-methylimidazolium tetrafluoroborate([bmim]BF4) ionic liquids(ILs) as the extractant. The operating conditions, including H2WO4 amount, IL dose, H2 O2 volume, temperature, catalyst dosage, and types of sulfur compounds,were systematically researched. The desulfurization rate could reach 98.46% for removing dibenzothiophene(DBT) from the model oil under optimal reaction conditions. In addition, the catalytic activity was slightly decreased after five recycles of catalysts. The reaction kinetics analysis shows that the oxidative desulfurization system was in accord with the first-order reaction kinetics equation. The mechanism of oxidative desulfurization was proposed. 展开更多
关键词 oxidative DESULFURIZATION WO3/g-C3N4 heterogeneous CATALYSIS EXTRACTANT
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Probing the intrinsic catalytic activity of carbon nanotubes for the metal-free oxidation of aromatic thiophene compounds in ionic liquids 预览
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作者 Qingqing Gu Yuxiao Ding +4 位作者 Zigeng Liu Yangming Lin Robert Schlogl Saskia Heumann Dangsheng Su 《能源化学:英文版》 SCIE EI CAS CSCD 2019年第5期131-137,共7页
A metal-free catalytic system combining oxidized carbon nanotubes (oCNTs) and ionic liquids (ILs) is presented for the oxidation of aromatic thiophene compounds with H2O2 as an oxidant. The oCNTs exhibit impressively ... A metal-free catalytic system combining oxidized carbon nanotubes (oCNTs) and ionic liquids (ILs) is presented for the oxidation of aromatic thiophene compounds with H2O2 as an oxidant. The oCNTs exhibit impressively high activity and stability in the system, which show an even better performance than those of some reported metal catalysts. The ILs are proved to have indispensable influence on the enhanced catalytic performance of the oCNTs. Detailed characterization by TG-MS and XPS demonstrates that the carbonyl groups are the active sites for the oxidation process, which is further supported by the deactivation and the model catalysts experiments. The quantitative analysis of different oxygen groups in oCNTs could be achieved by an isothermal temperature programmed TG-MS method. The concentration of carbonyl groups is 1.46 mmol per 1 g oCNTs and the tuiriover frequency of oCNTs could also be obtained (10.7 h^-1 in the presence of OmimPF6). H2O2 decomposition experiments combined with the EPR results reveal that the presence of OmimPF6 can avoid the intermediate HO· to form O2 and then improve the catalytic performance of oCNTs for the oxidation of dibenzothiophene. 展开更多
关键词 Carbon nanotubes METAL-FREE catalysis Oxidative DESULFURIZATION Ionic liquids
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Toxicity and mechanisms of action of titanium dioxide nanoparticles in living organisms
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作者 Jing Hou Luyao Wang +4 位作者 Chunjie Wang Songlin Zhang Haiqiang Liu Shiguo Li Xiangke Wang 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第1期40-53,共14页
Titanium dioxide nanoparticles(TiO2 NPs) are one of the most widely used nanomaterials in the consumer products, agriculture, and energy sectors. Their large demand and widespread applications will inevitably cause da... Titanium dioxide nanoparticles(TiO2 NPs) are one of the most widely used nanomaterials in the consumer products, agriculture, and energy sectors. Their large demand and widespread applications will inevitably cause damage to organisms and ecosystems. A better understanding of TiO2 NP toxicity in living organisms may promote risk assessment and safe use practices of these nanomaterials. This review summarizes the toxic effects of TiO2 NPs on multiple taxa of microorganisms, algae, plants, invertebrates, and vertebrates. The mechanism of TiO2 NP toxicity to organisms can be outlined in three aspects: The Reactive Oxygen Species(ROS)produced by TiO2 NPs following the induction of electron–hole pairs; cell wall damage and lipid peroxidation of the cell membrane caused by NP-cell attachment by electrostatic force owing to the large surface area of TiO2 NPs; and TiO2 NP attachment to intracellular organelles and biological macromolecules following damage to the cell membranes. 展开更多
关键词 TiO2 nanoparticles PARTICLE size EXPOSURE OXIDATIVE stress LIVING ORGANISMS
Neuroprotective effect of Paeoniae Radix Rubra on hippocampal CA1 region of mice induced by transient focal cerebral ischemia via anti-gliosis and anti-oxidant activity
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作者 Xiao-lu Zhu Bing-chun Yan +4 位作者 Cheng Tang Guo-wei Qiu Yao Wu Jie Wang Ping Bo 《中草药:英文版》 CAS 2019年第1期86-91,共6页
Objective: Stroke is the second leading cause of death worldwide. This study aimed to investigate the neuroprotective effect of Paeoniae Radix Rubra(PRR) on ischemic stroke of mice.Methods: The focal ischemic stroke m... Objective: Stroke is the second leading cause of death worldwide. This study aimed to investigate the neuroprotective effect of Paeoniae Radix Rubra(PRR) on ischemic stroke of mice.Methods: The focal ischemic stroke model was produced via middle cerebral artery occlusion. The experimental mice were divided into four groups: vehicle-sham group, PRR-sham group, vehicle-ischemia group, and PRR-treated ischemia group. The cerebral infarction volume was detected with TTC staining.The number of neurons in the hippocampal CA1 of the ischemic side, and the activation of astrocytes and microglia were observed via immunohistochemical staining. Western blotting was used to determine the expression changes of SOD1, SOD2, and Catalase protein levels in the hippocampus.Results: PRR significantly reduced the cerebral infarct volume induced by ischemic injury and inhibited the astrocytes and microglia activation in the hippocampal CA1 region. The decreased levels of SOD1,SOD2, and Catalase that was induced by ischemic reperfusion were simultaneously improved after PRR treatment.Conclusion: PRR improved neuronal injuries that were induced by transient cerebral ischemia via inhibiting gliosis and elevating anti-oxidants. 展开更多
关键词 cerebral ISCHEMIA/REPERFUSION GLIOSIS NEUROPROTECTIVE OXIDATIVE stress Paeoniae RADIX Rubra
MCPIP1 mediates inflammatory responses induced by lipopolysaccharide and lipoteichoic acid in bovine mammary epithelial cells
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作者 Shaopei Qi Lixue Wang +3 位作者 Xiao Chang Yanfen Zhang Yong Zhang Ming-Qing Gao 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第2期150-158,共9页
Monocyte chemoattractant protein-induced protein 1(MCPIP1)is a kind of zinc finger RNA binding protein,which exerts immune responses in a variety of cell types.However,the role of MCPIP1 in bovine mammary epithelial c... Monocyte chemoattractant protein-induced protein 1(MCPIP1)is a kind of zinc finger RNA binding protein,which exerts immune responses in a variety of cell types.However,the role of MCPIP1 in bovine mammary epithelial cells during mastitis has not been studied.In this study,we explored the functions of MCPIP1 in the inflammatory process induced by virulence factors of pathogens in bovine mammary alveolar cell-T(MAC-T)cell line.Our results showed that MCPIP1 was significantly highly expressed both in the mammary tissue of dairy cows with mastitis and in inflammatory MAC-T cells induced by lipopolysaccharide(LPS)or lipoteichoic acid(LTA).Furthermore,we found that overexpression of MCPIP1 in MAC-T cells abated the LPS-induced increase at the gene expression levels of inflammatory mediators tumor necrosis factor-α-α,interleukin(IL)-1β,IL-6 and IL-8,enhanced the LPS-and LTA-induced inhibition of epithelial proliferation and promoted the LPS-and LTA-induced oxidative and DNA damage.These findings indicated that MCPIP1 has an enormous potential in regulating the inflammatory response of bovine mammary epithelial cells during infection and may provide an effective therapeutic target for bovine mastitis to reduce the damage caused by inflammatory reactions. 展开更多
关键词 MCPIP1 BOVINE MAMMARY EPITHELIAL cell inflammatory response DNA DAMAGE OXIDATIVE DAMAGE
Osthole prevents tamoxifen-induced liver injury in mice
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作者 Wen-bo Zhou Xin-xin Zhang +2 位作者 Yun Cai Wu Sun Hao Li 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第5期608-619,共12页
Tamoxifen (TMX) is an antiestrogen drug that is used in the treatment and prevention of all stages of estrogen-dependent breast cancer. Adverse effects of TMX include hepatotoxicity. In this study, we investigated the... Tamoxifen (TMX) is an antiestrogen drug that is used in the treatment and prevention of all stages of estrogen-dependent breast cancer. Adverse effects of TMX include hepatotoxicity. In this study, we investigated the therapeutic effects of osthole, isolated from medicinal plants especially Fructus Cnidii, on TMX-induced acute liver injury in mice. Mice were injected with osthole (100mg/kg, ip) or vehicle, followed by TMX (90mg/kg, ip) 24h later. We showed that a single injection of TMX-induced liver injury and oxidative stress. Pretreatment with osthole attenuated TMX-induced liver injury evidenced by dose-dependent reduction of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities. Pretreatment with osthole also blunted TMX-induced oxidative stress, evidenced by significant increase of reduced glutathione (GSH) as well as reduction of malondialdehyde (MDA) and hydrogen peroxide (H2O2). Consistently, osthole significantly enhanced the expressions of antioxidant genes (GPX1, SOD2, GCL-c, and G6pdh), but suppressed those of pro-oxidant genes (NOX2 and ACOX). Furthermore, osthole inhibited the production of inflammatory cytokines, reduced the metabolic activation of TMX, and promoted its clearance. We further revealed that osthole elevated hepatic cAMP and cGMP levels, but inhibition of PKA or PKG failed to abolish the hepatoprotective effect of osthole. Meanwhile, prominent phosphorylation of p38 was observed in liver in response to TMX, which was significantly inhibited by osthole. Pretreatment with SB203580, a p38 inhibitor, significantly attenuated TMX-induced increase of ALT and AST activities, reduced oxidative stress, and reversed the alterations of gene expression caused by TMX. Moreover, pretreatment with L-buthionine sulfoximine (BSO), an inhibitor of GSH synthesis, partly reversed the effect of osthole on TMX-induced liver injury. Consistently, pretreatment with N-acetyl-L-cysteine (NAC) significantly attenuated TMX-induced increase in ALT and AST activities. Notably, both 展开更多
关键词 HEPATOTOXICITY TAMOXIFEN oxidative stress OSTHOLE MAPK SB203580 L-buthionine sulfoximine N-ACETYL-L-CYSTEINE
Mitochondria-targeted antioxidant peptide SS-31 mediates neuroprotection in a rat experimental glaucoma model
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作者 Xiaoqiong Wu Yu Pang +6 位作者 Zhilin Zhang Xiabin Li Chao Wang Yingqing Lei Ailing Li Ling Yu Jian Ye 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第4期411-421,共11页
To investigate the neuroprotective effects of the mitochondria-targeted antioxidant Szeto-Schiller peptide 31 (SS-31) in a rat experimental glaucoma model, SS-31 was intraperitoneally (IP) injected into Sprague-Dawley... To investigate the neuroprotective effects of the mitochondria-targeted antioxidant Szeto-Schiller peptide 31 (SS-31) in a rat experimental glaucoma model, SS-31 was intraperitoneally (IP) injected into Sprague-Dawley rats, followed by intracameral injection of polystyrene microspheres to induce elevated intraocular pressure (IOP). After 6 weeks, electroretinography (ERG) and flash visual-evoked potentials (F-VEPs) were recorded to assess retinal function. Hematoxylin-eosin staining was performed on retinal cross-sections to measure ganglion cell complex (GCC) thickness. Apoptotic retinal cells were assessed by TUNEL staining. Brn3a-positive retinal ganglion cells (RGCs) were counted in retinal flat mounts via immunofluorescence. The retinal total SOD, SOD2, and MDA expression levels were assessed in retinal tissue homogenates. The cyt c, Baxz and Bcl-2 protein levels in rat retinas were detected by western blot analysis. Bax and Bcl-2 expressions were also evaluated using immunohistochemistry in paraffinized sections. Our results showed that the rats that received microsphere injection developed elevated IOP. SS-31 ameliorated the reductions in the a- and b-wave amplitudes on ERG and the F-VEP amplitude in glaucomatous eyes. GCC thickness was preserved, TUNEL-positive cells were decreased in the retina, and Brn3a-positive RGCs were in creased in the SS-31-treated glaucoma group compared with those in the non-treated glaucoma group. SS-31 significantly reduced MDA levels and increased SOD2 levels after glaucoma induction. Significant suppression of cyt c release, upregulation of Bcl-2, and downregulation of Bax were observed following SS-31 administration. In summary, SS-31 exerts neuroprotective effects in this experimental glaucoma model by inhibiting mitochondrial dysfunction and therefore represents a promising therapeutic agent for glaucoma. 展开更多
关键词 SS-31 GLAUCOMA NEUROPROTECTION oxidative stress MITOCHONDRIAL DYSFUNCTION
The autophagy gene ATG8 affects morphogenesis and oxidative stress tolerance in Sporisorium scitamineum 预览
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作者 ZHANG Bin CUI Guo-bing +5 位作者 CHANG Chang-qing WANG Yi-xu ZHANG Hao-yang CHEN Bao-shan DENG Yi-zhen JIANG Zi-de 《农业科学学报:英文版》 SCIE CAS CSCD 2019年第5期1024-1034,共11页
The basidiomycetous fungus Sporisorium scitamineum causes sugarcane smut that leads to severe economic losses in the major sugarcane growing areas in China,India and Brazil,etc.Autophagy is a conserved pathway in euka... The basidiomycetous fungus Sporisorium scitamineum causes sugarcane smut that leads to severe economic losses in the major sugarcane growing areas in China,India and Brazil,etc.Autophagy is a conserved pathway in eukaryotes for bulk degradation and cellular recycling,and was shown to be important for fungal cell growth,development,and pathogenicity.However,physiological function of autophagy has not been studied in S.scitamineum.In this study,we identified a conserved Atg8 protein,named as SsAtg8 and characterized its function.Our results showed that autophagy was blocked in the ssatg8Δ mutant,in nitrogen starvation.The ssatg8Δ mutant formed pseudohypha frequently and was hypersensitive to oxidative stress.However,mating or filamenation was unaffected in the ssatg8Δ mutant in vitro.Overall we demonstrate that autophagy is dispensable for S.scitamineum mating/filamentation,while critical for oxidative stress tolerance and proper morphology in sporidial stage. 展开更多
关键词 Atg8 AUTOPHAGY FUNGUS Sporisorium scitamineum MORPHOGENESIS OXIDATIVE stress TOLERANCE
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Telfairia occidentalis (Cucurbitaceae) pulp extract mitigates rifampicin-isoniazid-induced hepatotoxicity in an in vivo rat model of oxidative stress
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作者 Lucky Legbosi Nwidu Yibala Ibor Oboma 《结合医学学报:英文版》 CAS CSCD 2019年第1期46-56,共11页
Objective:Drug-induced liver injury complicates antituberculosis drug treatment and is a leading cause of death worldwide. The aim of this study is to establish the ethnomedicinal claim of hepatoprotective effects of ... Objective:Drug-induced liver injury complicates antituberculosis drug treatment and is a leading cause of death worldwide. The aim of this study is to establish the ethnomedicinal claim of hepatoprotective effects of fruit pulp extract of Telfairia occidentalis against rifampicin (RIF) and isoniazid (INH)-induced oxidative stress in rats.Methods:T. occidentalis pulp extract (TOPE) (125-500mg/kg) and silymarin (50mg/kg) were evaluated in an induced hepatotoxicity model of oxidative stress in Wistar rats by intoxication with RIF and INH (100mg/kg each) orally for 60d. Markers indicating oxidative stress and hepatic damage such as alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were assessed. Biomarkers of antioxidant status, including catalase, glutathione reductase, glutathione peroxidase and superoxide dismutase, and marker of lipid peroxidation, malondialdehyde (MDA), were assayed using standard procedures. The hematological profile, lipid profile, serum markers for kidney function and histopathological examination were also assessed.Results:Intoxication with RIF and INH markedly reduced the hematological indices and elevated the biochemical enzyme markers (AST, ALT and ALP, P<0.001) and lipid profile (P<0.001), while antioxidant biomarkers were significantly (P<0.01) depressed and MDA was elevated. However, pretreatment with TOPE significantly (P<0.001) alleviated this alteration and sustained the antioxidant potentials. The histopathological morphology supports the biochemical evidence of hepatoprotection.Conclusion:Current study is indicative of potential antioxidant activity, hepatoprotective effects and plausible therapeutic alleviation of RIF-INH-induced hepatotoxicity of TOPE in laboratory animals. 展开更多
关键词 Telfairia OCCIDENTALIS PULP EXTRACT Oxidative stress Antioxidant Lipid PEROXIDATION HEPATOPROTECTIVE
Continuous Positive Airway Pressure Effectively Alleviates Arrhythmias in Patients with Obstructive Sleep Apnea:Possible Relationship with Counteracting Oxidative Stress 预览
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作者 Xiao-ting WANG Gang ZHAO +7 位作者 Ling TU Zhi-yong YUE Zhen-hua LIU Jie HAN Kun GAO Xuan-chen ZHOU Shuai XU Jian-feng LI 《当代医学科学(英文)》 SCIE CAS 2019年第1期52-58,共7页
This work is aimed at exploring the clinical efficacy of continuous positive airway pressuie(CPAP)in treatment of patients with arrhythmias combined with obstructive sleep apnea(OSA).Through evaluating serum native th... This work is aimed at exploring the clinical efficacy of continuous positive airway pressuie(CPAP)in treatment of patients with arrhythmias combined with obstructive sleep apnea(OSA).Through evaluating serum native thiol,malonaldehyde(MDA)and nicotinamide adenine dinucleotide phosphate oxidase(NADPH oxidase)in these patients and describing the effects on oxidative parameters of CPAP therapy for 3 months,we confirmed the impact of oxidative stress on arrhythmias.A total of 64 patients with OSA combined with arrhythmias were collected from April 2014 to April 2017 with full clinical information.Patients were divided into two groups(paired experiment design):32 patients in group A(control group),who received unchanged anti-arrhythmia treatment and 32 patients in group B,who were subjected to unchanged pharmacological anti-arrhythmia therapy combined with CPAP.OSA related parameters were compared between the two groups after 3-month therapy.And the levels of parameters of oxidative stress in patients were measured before and after CPAP therapy.After 3 months of CPAP therapy,compared with the control group,the percentage of sage N3(NREM 3)and stage R(REM)in total sleep time was significantly increased,while apnea-hypopnea index(AHI)and the Epworth Sleepiness Scale(ESS)score were evidently decreased.Meanwhile,the lowest oxygen saturation(LSpCh)was also elevated after CPAP treatment for 3 months.The CPAP therapy significantly prevented the occurrence of arrhythmias(P<0.05).Both the MDA level and NADPH oxidase levels were significantly lower in the group B than in the group A(P<0.05).But serum native thiol was improved by CPAP treatment(P<0.05).In conclusion,proper use of CPAP therapy provides significant benefits for the treatment of arrhythmia in patients with OSA. 展开更多
关键词 OBSTRUCTIVE SLEEP APNEA ARRHYTHMIAS oxidative stress POLYSOMNOGRAPHY continuous positive AIRWAY pressure
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Physiological Effects of MgO and ZnO Nanoparticles on the Citrus maxima
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作者 肖炼 王守霞 +2 位作者 YANG Daoyong ZOU Zhengkang 李俊丽 《武汉理工大学学报:材料科学英文版》 SCIE EI CAS 2019年第1期243-253,共11页
Toxicity of MgO and ZnO nanoparticles at concentrations of 250, 500 or 1 000 mg/L for Citrus maxima seedlings was investigated to evaluate the potentiality of their use as nano-fertilizers. Uptake and translocation of... Toxicity of MgO and ZnO nanoparticles at concentrations of 250, 500 or 1 000 mg/L for Citrus maxima seedlings was investigated to evaluate the potentiality of their use as nano-fertilizers. Uptake and translocation of metal oxide nanoparticles and lipid peroxidation were measured and compared with those of plants exposed to the highest equivalent concentrations of Mg2+ and Zn2+. MgO nanoparticles were translocated from roots to shoots, while translocation of ZnO nanoparticles was low. Exposure to Mg2+ and MgO at all concentrations entailed severe toxicity and strong oxidative stress. ZnO nanoparticles showed only mild toxicity, while Zn2+ caused leaf vein chlorosis and strong oxidative stress to plant shoots. In conclusion, the toxicity of MgO nanoparticles to the plant resulted from the dissolved Mg2+ concentration, while that of ZnO nanoparticles was not correlated with the dissolved Zn2+ concentration. Our findings are significant for development and application of MgO and ZnO nanoparticles as nano-fertilizers in agriculture. 展开更多
关键词 MGO and ZnO NANOPARTICLES CITRUS MAXIMA TRANSLOCATION lipid PEROXIDATION oxidative stress
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