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Albumin-to-alkaline phosphatase ratio: A novel prognostic index of overall survival in cholangiocarcinoma patients after surgery 预览
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作者 Jian-Ping Xiong Jun-Yu Long +6 位作者 Wei-Yu Xu Jin Bian Han-Chun Huang Yi Bai Yi-Yao Xu Hai-Tao Zhao Xin Lu 《世界胃肠肿瘤学杂志:英文版(电子版)》 CAS 2019年第1期39-47,共9页
AIM To clarify the prognostic significance of preoperative albumin-to-alkaline phosphatase ratio (AAPR) in cholangiocarcinoma (CCA) subjects receiving surgery. METHODS In this retrospective study, we included 303 CCA ... AIM To clarify the prognostic significance of preoperative albumin-to-alkaline phosphatase ratio (AAPR) in cholangiocarcinoma (CCA) subjects receiving surgery. METHODS In this retrospective study, we included 303 CCA patients receiving surgery without preoperative therapy between 2002 and 2014. Clinicopathological characteristics (including AAPR) were analyzed to determine predictors of postoperative overall survival and recurrence-free survival (RFS). In addition, univariate and multivariate Cox proportional hazards models were conducted, followed by application of time-dependent receiver operating curves to identify the optimal cut-off. RESULTS Univariate and multivariate analyses revealed both decreased overall survival [hazard ratio (HR): 2.88, 95%CI: 1.19-5.78] and recurrence-free survival (HR: 2.31, 95%CI: 1.40–3.29) in patients with AAPR < 0.41 compared to those with AAPR ≥ 0.41. The optimal cut-off of AAPR was 0.41. Of the 303 subjects, 253 (83.5%) had an AAPR over 0.41. The overall 1-, 3- and 5-year survival rates were 70.2%, 38.0% and 16.5%, respectively in the low (< 0.41) AAPR group, which were significantly lower than those in the high (≥ 0.41) AAPR group (81.7%, 53.9%, and 33.4%, respectively)(P < 0.0001). Large tumor size, multiple tumors, and advanced clinical stage were also identified as significant predictors of poor prognosis. CONCLUSION Our outcomes showed that AAPR was a potential valuable prognostic indicator in CCA patients undergoing surgery, which should be further confirmed by prospective studies. Moreover, it is necessary to investigate the mechanisms concerning the correlation of low AAPR with poor post-operative survival in CCA patients. 展开更多
关键词 Albumin-to-alkaline PHOSPHATASE RATIO CHOLANGIOCARCINOMA Prognosis SURGERY Survival
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The inhibitory mechanism of aurintricarboxylic acid targeting serine/threonine phosphatase Stpl in Staphylococcus aureus: insights from molecular dynamics simulations
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作者 Ting-ling Liu Teng Yang +4 位作者 Mei-na Gao Kai-xian Chen Song Yang Kun-qian Yu Hua-liang Jiang 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第6期850-858,共9页
Serine/threonine phosphatase (Stpl) is a member of the bacterial Mg^2+- or Mn^2+- dependent protein phosphatase/protein phosphatase 2C family, which is involved in the regulation of Siophylococcus aureus virulence. Au... Serine/threonine phosphatase (Stpl) is a member of the bacterial Mg^2+- or Mn^2+- dependent protein phosphatase/protein phosphatase 2C family, which is involved in the regulation of Siophylococcus aureus virulence. Aurintricarboxylic acid (ATA) is a known Stpl infiibilur wilfi an IC50 of 1,03 mM, but its inhibitory mechanism has not been elucidated in detail because the Stpl-ATA cocrystal structure has not been determined thus far. In this study, we performed 400 ns molecular dynamics (MD) simulations of the apo Stpl and Stpl ATA complex modds. During MD simulations, the flap subdomain of the Stpl-ATA complex experienced a clear conformational transition from an open state to a closed state, whereas the flap domain of apo-stp1 changed from an open state to a semi-open state. In the Stpl-ATA complex model, the hydrogen bond (H-bond) between D137 and NT42 disappeared, whereas critical H-bond intpmrtions wprp formed bptwppn Q160 and H13, Q160/R161 and ATA, well N162 and D198. Finally, four residues (D137, N142,Q160, and R161) in Stpl were mutated to alanine and the mutant enzymes were assessed using phosphate enzyme activity assays, which confirmed their important roles in maintaining Stpl activity. This study indicated the inhibitory mechanism of ATA targeting Stpl using MD simulations and sheds light on the future design of allosteric Stpl inhibitors. 展开更多
关键词 antimicrobial resistance STAPHYLOCOCCUS AUREUS serine/threonine PHOSPHATASE aurintricarboxylic acid molecular dynamics simulations flap subdomairji
3D-QSAR and Surflex Docking Studies of a Series of Alkaline Phosphatase Inhibitors 预览
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作者 舒茂 武涛 +3 位作者 王必武 李静 徐春媚 林治华 《结构化学》 SCIE CAS CSCD 2019年第1期7-16,1共11页
Alkaline phosphatases(APs) include the placental AP(PLAP), germ cell AP(GCAP), intestinal AP(IAP) and tissue nonspecific AP(TNAP). Over expression of TNAP in smooth muscle cells of kidney and vessels provokes the prog... Alkaline phosphatases(APs) include the placental AP(PLAP), germ cell AP(GCAP), intestinal AP(IAP) and tissue nonspecific AP(TNAP). Over expression of TNAP in smooth muscle cells of kidney and vessels provokes the progress of such serious diseases as end-stage renal disease, idiopathic infantile arterial calcification, ankylosis, osteoarthritis and diabetes. In order to design and optimize the potent TNAP inhibitors, comparative molecular field analysis(CoMFA) and comparative molecular similarity indices analysis(CoMSIA) were used to analyze 3D structure-activity relationships(3D-QSAR) of TNAP inhibitors. The 3D-QSAR model(CoMFA with q^2 = 0.521, r^2 = 0.930;CoMSIA with q^2 = 0.529, r^2 = 0.933) had a good predictability. Surflex-dock was used to reveal the binding mode between the inhibitors and TNAP protein. CoMFA, CoMSIA and docking results provide guidance for the discovery of TNAP inhibitors. Finally, eight new compounds as potential TNAP inhibitors were designed. 展开更多
关键词 3D-QSAR surflex-dock ALKALINE PHOSPHATASE INHIBITORS
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Association between Serum Alkaline Phosphatase and Carotid Atherosclerosis in a Chinese Population: A Community-based Cross-sectional Study 预览
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作者 YE Yi Cong LIU Hua Min +1 位作者 ZHOU Yong ZENG Yong 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第6期446-453,共8页
Objective This study aimed to investigate the relationship between alkaline phosphatase (ALP) and comm on carotid intima media thick ness (IMT), carotid plaque, and extracra nial carotid artery stenosis (ECAS). Method... Objective This study aimed to investigate the relationship between alkaline phosphatase (ALP) and comm on carotid intima media thick ness (IMT), carotid plaque, and extracra nial carotid artery stenosis (ECAS). Methods A total of 3,237 participa nts aged > 40 years were recruited from Jidong comm unity in 2013-2014. Participants were divided into five quintile groups based on their serum ALP levels. Carotid atherosclerosis was assessed using ultrasou nd. Abn ormal IMT, carotid plaque, and ECAS were defi ned as IMT > 0.9 mm, IMT > 1.5 mm, and > 50% stenosis in at least one extracra nial carotid artery, respectively. Results Common carotid IMT values and the prevale nee of carotid plaque in creased across serum ALP quin tiles. Higher ALP quin tiles were correlated with an in creased risk of abn ormal IMT [fourth quintile: odds ratio (OR) 1.78, 95% confidence interval (CI) 1.13-2.82, P = 0.0135;fifth quintile: OR = 1.82, 95% CI: 1.15-2.87, P = 0.0110] and ECAS compared to the lowest quintile (fifth quintile: OR = 1.47, 95% CI: 1.09-1.97, P = 0.0106). The association between ALP and prevalence of carotid plaque became insign ifica nt after adjustme nt for con founders. Conclusion Serum ALP levels were independently associated with abnormal common carotid IMT and ECAS. These conclusions need to be further corroborated in future prospective cohort studies. 展开更多
关键词 Alkaline PHOSPHATASE Atherosclerosis INTIMA media thickness CAROTID plaque EXTRACRANIAL CAROTID artery STENOSIS
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白三叶降解对陕西地区苹果园土壤酶活性的影响 预览
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作者 杨文权 卢彪儒 +2 位作者 程宇阳 魏倩倩 寇建村 《草业科学》 CAS CSCD 2019年第2期295-303,共9页
为了解白三叶(Trifolium repens)降解对苹果园土壤酶活性的影响,在苹果典型优生区—陕西的洛川和旬邑苹果园中进行白三叶埋置降解试验,研究白三叶降解后对不同地区苹果园土壤蛋白酶、脲酶、蔗糖酶和碱性磷酸酶活性的影响。结果表明,苹... 为了解白三叶(Trifolium repens)降解对苹果园土壤酶活性的影响,在苹果典型优生区—陕西的洛川和旬邑苹果园中进行白三叶埋置降解试验,研究白三叶降解后对不同地区苹果园土壤蛋白酶、脲酶、蔗糖酶和碱性磷酸酶活性的影响。结果表明,苹果园中埋置白三叶后,除降解3 个月时的碱性磷酸酶活性和旬邑降解6 个月时的脲酶活性无变化外,其余处理的蛋白酶、脲酶、蔗糖酶和碱性磷酸酶的活性均显著提高(P < 0.05);在白三叶埋置6 个月后,土壤蛋白酶和碱性磷酸酶活性增加幅度大;埋置的白三叶降解后对不同地区苹果园土壤酶活性影响不同,对洛川苹果园的影响较旬邑苹果园大;相关性分析表明,土壤蔗糖酶和脲酶之间存在显著的正相关。说明埋置的白三叶降解可以提高土壤酶的活性,在一定程度上改善土壤理化性质,但此作用与苹果栽培地区和白三叶降解时间有一定的关系。 展开更多
关键词 黄土高原 果园种草 埋置 蛋白酶 脲酶 蔗糖酶 碱性磷酸酶
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Diabetic neuropathy research: from mouse models to targets for treatment 预览
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作者 Vuong M. Pham Shinji Matsumura +2 位作者 Tayo Katano Nobuo Funatsu Seiji Ito 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1870-1879,共10页
Diabetic neuropathy is one of the most serious complications of diabetes, and its increase shows no sign of stopping. Furthermore, current clinical treatments do not yet approach the best effectiveness. Thus, the deve... Diabetic neuropathy is one of the most serious complications of diabetes, and its increase shows no sign of stopping. Furthermore, current clinical treatments do not yet approach the best effectiveness. Thus, the development of better strategies for treating diabetic neuropathy is an urgent matter. In this review, we first discuss the advantages and disadvantages of some major mouse models of diabetic neuropathy and then address the targets for mechanism-based treatment that have been studied. We also introduce our studies on each part. Using stem cells as a source of neurotrophic factors to target extrinsic factors of diabetic neuropathy, we found that they present a promising treatment. 展开更多
关键词 brain derived NEUROTROPHIC FACTOR DIABETES extrinsic FACTORS NERVE growth FACTOR NERVE regeneration NEUROTROPHIC FACTORS NON-OBESE type 2 DIABETES phosphatase and tensin homolog stem cell streptozotocin
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Differential localizations of protein phosphatase 1 isoforms determine their physiological function in the heart
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作者 Ruijie Liu Christian Miller +2 位作者 Christiana D'Annibale Kimberly Vo Ashley Jacobs 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第3期323-330,共8页
Protein phosphatase 1 isoforms α,β,and γ(PP1α,PP1β,and PP1γ)are highly homologous in the catalytic domains but have distinct subcellular localizations.In this study,we utilized both primary cell culture and knoc... Protein phosphatase 1 isoforms α,β,and γ(PP1α,PP1β,and PP1γ)are highly homologous in the catalytic domains but have distinct subcellular localizations.In this study,we utilized both primary cell culture and knockout mice to investigate the isoform-specific roles of PP1s in the heart.In both neonatal and adult cardiac myocytes,PP1β was mainly localized in the nucleus,compared to the predominant presence of PP1α and PP1γ in the cytoplasm.Adenovirus-mediated overexpression of PP1α led to decreased phosphorylation of phospholamban,which was not influenced by overexpression of either PP1β or PP1γ.Interestingly,only cardiac-specific knockout of PP1β resulted in increased HDAC7 phosphorylation,consistent with the predominant nuclear localization of PP1β.Functionally,deletion of either PP1 isoform resulted in reduced fractional shortening in aging mice,however only PP1β deletion resulted in interstitial fibrosis in mice as early as 3 weeks of age.Deletion of neither PP1 isoform had any effect on pathological cardiac hypertrophy induced by 2 weeks of pressure overload stimulation.Together,our data suggest that PP1 isoforms have differential localizations to regulate the phosphorylation of their specific substrates for the physiological function in the heart. 展开更多
关键词 cardiac function HISTONE DEACETYLASE PHOSPHOLAMBAN protein PHOSPHATASE 1
Protein tyrosine phosphatase 1B inhibitory activities of ursane-type triterpenes from Chinese raspberry, fruits of Rubus chingii
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作者 ZHANG Xiang-Yu LI Wei +3 位作者 WANG Jian LI Ning CHENG Mao-Sheng KOIKE Kazuo 《中国天然药物:英文版》 SCIE CAS CSCD 2019年第1期15-21,共7页
Protein tyrosine phosphatase 1 B(PTP1 B) has led to an intense interest in developing its inhibitors as anti-diabetes, anti-obesity and anti-cancer agents. The fruits of Rubus chingii(Chinese raspberry) were used as a... Protein tyrosine phosphatase 1 B(PTP1 B) has led to an intense interest in developing its inhibitors as anti-diabetes, anti-obesity and anti-cancer agents. The fruits of Rubus chingii(Chinese raspberry) were used as a kind of dietary traditional Chinese medicine. The methanolic extract of R. chingii fruits exhibited significant PTP1 B inhibitory activity. Further bioactivity-guided fractionation resulted in the isolation of three PTP1 B inhibitory ursane-type triterpenes: ursolic acid(1), 2-oxopomolic acid(2), and 2α, 19α-dihydroxy-3-oxo-urs-12-en-28-oic acid(3). Kinetics analyses revealed that 1 was a non-competitive PTP1 B inhibitor, and 2 and 3 were mixed type PTP1 B inhibitors. Compounds 1-3 and structurally related triterpenes(4-8) were further analyzed the structure-activity relationship, and were evaluated the inhibitory selectivity against four homologous protein tyrosine phosphatases(TCPTP, VHR, SHP-1 and SHP-2). Molecular docking simulations were also carried out, and the result indicated that 1, 3-acetoxyurs-12-ene-28-oic acid(5), and pomolic acid-3β-acetate(6) bound at the allosteric site including α3, α6, and α7 helix of PTP1 B. 展开更多
关键词 Protein TYROSINE PHOSPHATASE 1B RASPBERRY RUBUS chingii TRITERPENE Ursane
Ectomycorrhizal and Saprotrophic Fungal Communities Vary Across mm-Scale Soil Microsites Differing in Phosphatase Activity
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作者 Aaron GODIN Denise BROOKS +1 位作者 Sue J. GRAYSTON Melanie D. JONES 《土壤圈:英文版》 SCIE CAS CSCD 2019年第3期344-359,共16页
To understand nutrient cycling in soils, soil processes and microorganisms need be better characterized. To determine whether specific trophic groups of fungi are associated with soil enzyme activity, we used soil imp... To understand nutrient cycling in soils, soil processes and microorganisms need be better characterized. To determine whether specific trophic groups of fungi are associated with soil enzyme activity, we used soil imprinting to guide mm-scale sampling from microsites with high and low phosphatase activities in birch/Douglas-fir stands. Study 1 involved sampling one root window per site at 12 sites of different ages(stands);study 2 was conducted at one of the stem-exclusion stands, at which 5 root windows had been installed. Total fungal and ectomycorrhizal(EM) fungal terminal-restriction fragment length polymorphism(TRFLP)fingerprints differed between high-and low-phosphatase activity microsites at 8 of 12 root windows across 12 sites. Where differences were detected, fewer EM fungi were detected in high-than low-phosphatase activity microsites. Using 5 root windows at one site,next-generation sequencing detected similar fungal communities across microsites, but the ratio of saprotrophic to EM fungal reads was higher in high-phosphatase activity microsites in the two windows that had low EM fungal richness. In windows with differences in fungal communities, both studies indicated that EM fungi were less successful than saprotrophic fungi in colonizing fine-scale,organic matter-rich microsites. Fine-scale sampling linked with in situ detection of enzyme activity revealed relationships between soil fungal communities and phosphatase activity that could not be observed at the scales employed by conventional approaches, thereby contributing to the understanding of fine-scale phosphorus cycling in forest soils. 展开更多
关键词 ectomycorrhizae fine SCALE FUNGAL communities PHOSPHATASE root windows saprotrophic fungi SOIL IMPRINTING
Age differences in associations of serum alkaline phosphatase and mortality among peritoneal dialysis patients
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作者 Juan Wu Xin-Hui Liu +5 位作者 Rong Huang Hai-Shan Wu Qun-Ying GUO Chun-Yan Yi Xue-Qing YU Xiao Yang 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第2期232-236,共5页
To the Editor:Serum alkaline phosphatase (ALP) is an important bone turnover marker in chronic kidney disease (CKD) patients, with studies indicating that higher serum ALP levels are associated with increased mortalit... To the Editor:Serum alkaline phosphatase (ALP) is an important bone turnover marker in chronic kidney disease (CKD) patients, with studies indicating that higher serum ALP levels are associated with increased mortality in hemodialysis (HD) patients.[1] Our previous study also showed that higher serum ALP levels were independently associated with all-cause and cardiovascular mortality in peritoneal dialysis (PD) patients. 展开更多
关键词 Serum ALKALINE PHOSPHATASE TURNOVER chronic KIDNEY
Exploration of Zinc(II)Complexes as Potent Inhibitors Against Protein Tyrosine Phosphatase IB
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作者 LI Xinhua YUAN Caixia +3 位作者 LU Liping ZHU Miaoli XING Shu FU Xueqi 《高等学校化学研究:英文版》 SCIE CAS CSCD 2019年第2期186-192,共7页
Although protein tyrosine phosphatases(PTPs)do not contain any metals,their activities can be inhibited by some metal complexes.Here we investigated the inhibition of two zinc complexes with Schiff base ligands agains... Although protein tyrosine phosphatases(PTPs)do not contain any metals,their activities can be inhibited by some metal complexes.Here we investigated the inhibition of two zinc complexes with Schiff base ligands against PTPs activity to explore their effect on the cellular metabolism.It has been found that they are potent inhibitors against four recombinant PIPs5 including protein tyrosine phosphatase 1B(PTP1B),T cell protein tyrosine phosphatase(TCPTP),megakaryocyte protein tyrosine phosphatase 2(PTP-MEG2)S and Src-homology phosphatase 1(SHP-1),with exception of Src-homology phosphatase 2(SHP-2).Moreover,tlaey showed moderate selective inhibition against PTP1B with the IG50 values of 0.15 and 0.36μmol/L.Meanwhile,the complexes also inhibited cellular phosphatase activities efficiently.Comparing the inhibitory potency over PTPs mediated by the zinc ion,we found that zinc complexes might be easily developed into potent and selective inhibitors against certain PTP by rationally modifying the organic ligands moieties. 展开更多
关键词 ZINC complex SCHIFF base Protein TYROSINE PHOSPHATASE INHIBITOR
不同水分条件下生物炭对红壤磷素形态及磷酸酶活性的影响 预览
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作者 夏丽丹 曹升 +3 位作者 张虹 胡华英 周垂帆 马祥庆 《农业环境科学学报》 CAS CSCD 北大核心 2019年第5期1101-1111,共11页
为探究土壤不同水分条件下生物炭对红壤磷素形态转化及磷酸酶活性的影响,以期为土壤磷素管理和生物炭合理利用提供参考。通过设置土壤不同含水量(33%、66%、100%)与生物炭添加量(0、0.5%、2%)进行培养试验,测定土壤的有效磷、各磷素形态... 为探究土壤不同水分条件下生物炭对红壤磷素形态转化及磷酸酶活性的影响,以期为土壤磷素管理和生物炭合理利用提供参考。通过设置土壤不同含水量(33%、66%、100%)与生物炭添加量(0、0.5%、2%)进行培养试验,测定土壤的有效磷、各磷素形态(Al-P、Ca-P、Fe-P、O-P)及土壤酸性磷酸酶与碱性磷酸酶活性。结果表明:生物炭的施入显著提高了土壤有效磷含量;在培养前期,生物炭主要增加土壤中难溶态的Al-P含量,这主要是由生物炭带来的可溶性磷进入土壤中转化所导致;在培养后期,水分与生物炭都能够在一定程度上活化土壤中的Ca-P、Fe-P与O-P,释放更多磷素。生物炭本身呈碱性,添加到土壤中,有效中和了土壤酸度,使得土壤pH值上升2.82~3.13个单位,土壤酸性磷酸酶活性下降。此外,淹水条件能够降低土壤的酸性磷酸酶与碱性磷酸酶活性。研究表明,生物炭的添加能够有效提高土壤pH值、有效磷含量,同时降低土壤酸性磷酸酶的活性。 展开更多
关键词 红壤 生物炭 磷素形态 磷酸酶
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Structural Insights into Substrate Selectivity, Catalytic Mechanism,and Redox Regulation of Rice Photosystem Ⅱ Core Phosphatase
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作者 Xiuying Liu Jingchao Chai +2 位作者 Xiaomin Ou Mei Li Zhenfeng Liu 《分子植物:英文版》 SCIE CAS CSCD 2019年第1期86-98,共13页
Photosystem Ⅱ (PSII)core phosphatase (PBCP)selectively dephosphorylates PSII core proteins including D1,D2,CP43,and PsbH.PBCP function is required for efficient degradation of the D1 protein in the repair cycle of PS... Photosystem Ⅱ (PSII)core phosphatase (PBCP)selectively dephosphorylates PSII core proteins including D1,D2,CP43,and PsbH.PBCP function is required for efficient degradation of the D1 protein in the repair cycle of PSII,a supramolecular machinery highly susceptible to photodamage during oxygenic photosynthesis.Here we present structural and functional studies of PBCP from Oryza sativa (OsPBCP).In a symmetrical homodimer of OsPBCP,each monomer contains a PP2C-type phosphatase core domain,a large motif characteristic of PBCPs,and two Small motifs around the active site.The large motif contributes to the formation of a substrate-binding surface groove,and is crucial for the selectivity of PBCP toward PSII core proteins and against the light-harvesting proteins.Remarkably,the phosphatase activity of OsPBCP is strongly inhibited by glutathione and H202.S-Glutathionylation of cysteine residues may introduce steric hindrance and allosteric effects to the active site.Collectively,these results provide detailed mechanistic insights into the substrate selectivity,redox regulation,and catalytic mechanism of PBCP. 展开更多
关键词 PSII CORE PHOSPHATASE structure SUBSTRATE SELECTIVITY redox regulation PSII repair cycle
生物炭-化肥配施对稻田土壤氮磷迁移转化的影响 预览
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作者 崔虎 王莉霞 +4 位作者 欧洋 阎百兴 韩露 李迎新 姜珊 《农业环境科学学报》 CAS CSCD 北大核心 2019年第2期412-421,共10页
在控制外源氮输入量相同的前提下,通过设置不同梯度生物炭配施量[N1+B0(磷酸氢二铵750kg·hm……-2);N2+B5(磷酸氢二铵583kg·hm^-2+生物炭5000kg·hm^-2);N3+B10(磷酸氢二铵416kg·hm^-2+生物炭10000kg·hm-2);N0+... 在控制外源氮输入量相同的前提下,通过设置不同梯度生物炭配施量[N1+B0(磷酸氢二铵750kg·hm……-2);N2+B5(磷酸氢二铵583kg·hm^-2+生物炭5000kg·hm^-2);N3+B10(磷酸氢二铵416kg·hm^-2+生物炭10000kg·hm-2);N0+B20(生物炭20000kg·hm^-2)],探讨无机肥减量配施生物炭对土壤氮、磷动态变化的影响。结果表明:4种处理土壤NH^+4-N和TP浓度均呈单峰变化趋势,分别于施肥后第9d(NH^+4-N)、25d(TP:N0+B20、N1+B0)和55d(TP:N2+B5、N3+B10)达到峰值;N2+B5和N3+B10处理土壤NO-3-N浓度呈双峰变化趋势,于施肥后第10d和55d达到峰值,而N0+B20和N1+B0处理土壤NO-3-N浓度施肥初期(1~10d)基本保持稳定状态,之后缓慢下降至稳定水平;N1+B0处理土壤TN浓度在施肥后1~55d内缓慢下降,此后呈单峰变化趋势,于施肥后第85d达到峰值;N2+B5、N3+B10和N0+B20处理土壤TN浓度呈双峰变化趋势,分别于施肥后的第9d和85d达到峰值。与单施无机肥N1+B0处理比较,配施生物炭N2+B5、N3+B10和N0+B20处理土壤TN和TP浓度分别提高了11.1%、33.3%、11.1%和40.0%、40.0%、40.0%,土壤脲酶和磷酸酶活性分别提高了25.0%、30.0%、10.0%和9.76%、18.3%、15.9%,表明生物炭较化肥具有更持久肥效。施肥初期,配施生物炭可提高土壤氮磷比;水稻成熟期,配施生物炭处理田面水氮磷比显著高于单施无机肥处理,能够持续地给水稻提供营养。N3+B10处理下水田面源污染物NO3--N、NH4+-N、TN和TP的输出负荷分别降低了29.6%、48.1%、49.7%和50.0%,是较适合东北黑土区水田的施肥方式。 展开更多
关键词 生物炭 脲酶 磷酸酶 输出负荷
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Combination of serum gamma-glutamyltransferase and alkaline phosphatase in predicting the diagnosis of asymptomatic choledocholithiasis secondary to cholecystolithiasis 预览
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作者 Yong Mei Li Chen +8 位作者 Peng-Fei Zeng Ci-Jun Peng Jun Wang Wen-Ping Li Chao Du Kun Xiong Kai Leng Chun-Lin Feng Ji-Hu Jia 《世界临床病例杂志》 2019年第2期137-144,共8页
BACKGROUND Gamma-glutamyltransferase(GGT)is one of the most important laboratory tests for the evaluation of liver damage.Through a long-term clinical observation of patients with secondary asymptomatic choledocholith... BACKGROUND Gamma-glutamyltransferase(GGT)is one of the most important laboratory tests for the evaluation of liver damage.Through a long-term clinical observation of patients with secondary asymptomatic choledocholithiasis,we found that most patients had abnormal GGT serum levels.AIM To investigate the combination of serum GGT and alkaline phosphatase(ALP)in predicting the diagnosis of asymptomatic choledocholithiasis secondary to cholecystolithiasis.METHODS In this retrospective cohort study,the clinical data of 829 patients with cholecystolithiasis admitted to the Third Affiliated Hospital of Zunyi Medical College from August 2014 to August 2017 were collected.Among these patients,151 patients had secondary asymptomatic choledocholithiasis and served as the observation group,and the remaining 678 cholecystolithiasis patients served as the control group.Serum liver function indexes were detected in both groups,and the receiver operating characteristic(commonly known as ROC)curves were constructed for markers showing statistical significances.The cutoff value,sensitivity,and specificity of each marker were calculated according to the ROC curves.RESULTS The overall incidence of asymptomatic choledocholithiasis secondary to cholecystolithiasis was 18.2%.The results of liver function indexes including serum aspartate aminotransferase,alanine aminotransferase,direct bilirubin and total bilirubin levels showed no significant differences between the two groups(P >0.05).However,the serum GGT and ALP levels were significantly higher in the observation group than in the control group(P<0.05).The ROC curve analysis showed that the area under the curve was 0.881(95%CI:0.830-0.932),0.647(95%CI:0.583-0.711)and 0.923(0.892-0.953)for GGT,ALP,and GGT+ALP,respectively.The corresponding cut-off values of GGT and ALP were 95.5 U/L and 151.5 U/L,sensitivity were 90.8%and 65.1%,and specificity were 83.6%and 59.8%,respectively.The sensitivity and specificity of GGT+ALP were 93.5%and 85.1%,respectively.CONCLUSION An abnormally elevated 展开更多
关键词 ASYMPTOMATIC CHOLEDOCHOLITHIASIS GAMMA-GLUTAMYLTRANSFERASE CHOLECYSTOLITHIASIS Alkaline PHOSPHATASE DIAGNOSIS Screening
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溶磷细菌筛选及对复垦土壤磷素有效性的评价 预览
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作者 乔志伟 腾飞龙 邵晓贵 《甘肃农业大学学报》 CAS CSCD 北大核心 2019年第2期139-146,共8页
【目的】为了评价溶磷微生物对提高复垦土壤有效性的作用.【方法】通过PVK平板稀释法从石灰性土壤中分离筛选出7株溶磷细菌,7株菌株的溶磷能力在296.5~563.5mg/kg之间,菌株的溶磷能力与溶磷圈直径(D)/菌落直径(d)的比值呈显著正相关;16s... 【目的】为了评价溶磷微生物对提高复垦土壤有效性的作用.【方法】通过PVK平板稀释法从石灰性土壤中分离筛选出7株溶磷细菌,7株菌株的溶磷能力在296.5~563.5mg/kg之间,菌株的溶磷能力与溶磷圈直径(D)/菌落直径(d)的比值呈显著正相关;16sRNA序列分析表明,W1、W6属于Enterobacter sp.,W2、W4属于Burkholderia sp.,W3、W5属于Rahnella sp.,W7属于Fluorescent pseudomonas.选择W1、W3、W4、W7作为试验菌株,研究其对复垦土壤有效磷、磷酸酶及各形态无机磷含量的影响.【结果】溶磷细菌可以提高复垦土壤有效磷含量、降低pH、增加磷酸酶含量,与接种灭菌菌液处理相比,4株溶磷细菌对复垦土壤有效磷增加在0.30~3.72mg/kg之间,降低pH0.03~0.09之间,土壤碱性和酸性磷酸酶提高幅度在9.68~34.22mg/kg和0.21~47.66mg/kg;溶磷细菌不仅可以增加复垦土壤Ca2-P、Ca8-P、Fe-P、Al-P的含量,而且可以显著减少复垦土壤Ca10-P,与接种灭菌菌液处理相比,4株菌株增加Ca2-P、Ca8-P、Fe-P、Al-P的范围分别在4.7%~33.8%、11.1%~26.0%、5.3%~24.1%、4.8%~30.0%,Ca10-P的减少幅度在12.9%~14.9%,溶磷细菌对O-P影响不显著.【结论】溶磷细菌在提高复垦土壤磷素有效性方面发挥着重要的作用. 展开更多
关键词 溶磷细菌 复垦土壤 无机磷形态 磷酸酶
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S1-3 Effects and Mechanisms of Depression on Bone Fracture Healing in Rats 预览
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作者 FU Zhi-jiang LIU Yong +3 位作者 GAO Yin ZHANG Wei GAO Yun LIU Zong-chao 《神经药理学报》 2018年第4期5-6,共2页
Objective:To explore the effect of depression on fracture healingand forecast its agnostic mechanism.Methods:Rats(Sprague Dawley,female,8-month-old,(242.6±11.2)g)wererandomly divided into Control(group A),Sham Co... Objective:To explore the effect of depression on fracture healingand forecast its agnostic mechanism.Methods:Rats(Sprague Dawley,female,8-month-old,(242.6±11.2)g)wererandomly divided into Control(group A),Sham Control(group B)and Depression(group C)groups.Upper 1/3 transverse tibia fracture model and intramedullaryfix were applied to Group A and C and sham operations(similar surgeries with no fracture and fix)were applied to Group B.The serum calcium(Ca),phosphorus(P)and alkaline phosphatase(ALP)were measured on day 7,14,and 21 after operation;the tibia image(DR)and pathology(HE stain)were examined onday14,28,and 56 after operation;the Biomechanics(three-point bending test)were implemented on day 35 and 56 after operation;and the Micro-CT were performed on day 28 and 56 after operation.Results:The tibia image(DR)showed no significant difference in callus volume between Groups A and C on Day 14 after operation(P>0.05,n=10),but had significant difference on Day 28 and 56 after operation(P<0.05,n=10).HE staining showed significantly worse recovery of the fracture zone in Group C than in Group A on Day 14,28,and 56 after operation.The results from three point bending experiment revealed a significantly worse recovery of all bending indices(stiffness,strength,deflection,load,and movement)of Group C than the control Group A on Day 35 and 56 after operation(P<0.05,n=10).Micro-CTalsoshowed that the osteogenesis indicators such as bone volume fraction,thickness of trabecular bone,and number of trabecular bone were significantly lower in Group C than that in the control group A on Day 28 and 56 after operation(P<0.05,n=10).The volume of interstitial bone trabecular space and area of trabecular bone of Group C was significantly greater than that of control Group A(P<0.05,n=10).The serum P and ALP concentrations of Group C were significantly lower than those of group Bon Day 7(P<0.05,P<0.01,n=10),14(P<0.05,P<0.01,n=10),and 21(P<0.05,P<0.01,n=10)after operation,while the serum Ca concentrations did not show any signifi cant 展开更多
关键词 DEPRESSION FRACTURE FRACTURE HEALING ALKALINE PHOSPHATASE RATS
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探讨2型糖尿病性骨质疏松患者骨代谢指标水平变化及其影响因素
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作者 贺立侠 金秀平 +1 位作者 刘佳明 万秋霞 《兰州大学学报:医学版》 CAS 2018年第6期45-49,共5页
目的探讨糖尿病性骨质疏松患者骨代谢指标的变化及其影响因素。方法选取住院2型糖尿病患者218例,记录患者年龄、糖尿病病程、血糖控制水平;测定空腹血糖(FPG)、糖化血红蛋白(HbA1c)、血钙(Ca)、血磷(P)、血碱性磷酸酶(ALP);测定身高、体... 目的探讨糖尿病性骨质疏松患者骨代谢指标的变化及其影响因素。方法选取住院2型糖尿病患者218例,记录患者年龄、糖尿病病程、血糖控制水平;测定空腹血糖(FPG)、糖化血红蛋白(HbA1c)、血钙(Ca)、血磷(P)、血碱性磷酸酶(ALP);测定身高、体重,计算体重指数(BMI);测定骨密度(BMD),记录相应的T、Z值。按骨密度结果分为骨质疏松组(A组),骨量减少组(B组),骨量正常组(C组)。结果骨质疏松在不同性别的检出率不同,女性患者骨质疏松的检出率大于男性(P <0.05);A、B组的年龄、病程、HbA1c明显大于C组(P <0.05);A组的FPG、ALP大于B、C组(P <0.05);A组的BMI低于B、C组(P <0.05);而3组间血Ca、血P差异无统计学意义(P>0.05)。结论糖尿病性骨质疏松患者骨代谢指标血Ca、血P无明显变化,而血ALP升高。年龄、FPG、HbA1c是骨质疏松的独立危险因素。 展开更多
关键词 糖尿病性骨质疏松 骨代谢 血钙 血磷 碱性磷酸酶
解磷菌培养的代谢产物初步研究 预览
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作者 李文谦 卢康 +1 位作者 茅燕勇 陈大兵 《生物化工》 2018年第6期84-86,90共4页
研究了解磷菌发酵液中的有机酸含量、磷酸酶活性、多糖含量、蛋白质含量及磷含量。结果表明,发酵液中有机酸含量最高达33.1mg/L,磷酸酶含量以酸性磷酸酶最高,为242.6μmol/(L·h),多糖含量最高达到54.2mg/L,蛋白质含量最高达51mg/L... 研究了解磷菌发酵液中的有机酸含量、磷酸酶活性、多糖含量、蛋白质含量及磷含量。结果表明,发酵液中有机酸含量最高达33.1mg/L,磷酸酶含量以酸性磷酸酶最高,为242.6μmol/(L·h),多糖含量最高达到54.2mg/L,蛋白质含量最高达51mg/L,磷含量最高达0.956mg/L。表明有机酸、磷酸酶、多糖、蛋白质等物质的分泌与枯草芽孢杆菌的解磷效果有关。 展开更多
关键词 解磷菌 代谢产物 磷酸酶
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灭螺药物作用下的螺类酶学研究
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作者 熊涛 赵琴平 +2 位作者 刘镕 蒋明森 董惠芬 《中国血吸虫病防治杂志》 CSCD 北大核心 2018年第2期237-240,243共5页
在灭螺药物作用机理的研究中,螺类酶学研究起着非常重要的作用。多种酶在药物作用前后活性的变化在大量文献中被报道,本文对灭螺药物作用下的螺类酶学研究进展作一综述。
关键词 螺类 酶学 能量代谢 转氨酶 磷酸酶 神经信号转导
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