期刊文献+
共找到16,944篇文章
< 1 2 250 >
每页显示 20 50 100
MGMT is down-regulated independently of promoter DNA methylation in rats with all-trans retinoic acidinduced spina bifida aperta 预览
1
作者 He-Nan Zhang Yi Guo +3 位作者 Wei Ma Jia Xue Wei-Lin Wang Zheng-Wei Yuan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第2期361-368,共8页
O6-methylguanine DNA methyltransferase (MGMT), a DNA repair enzyme, has been reported in some congenital malformations, but it is less frequently reported in neural tube defects. This study investigated MGMT mRNA expr... O6-methylguanine DNA methyltransferase (MGMT), a DNA repair enzyme, has been reported in some congenital malformations, but it is less frequently reported in neural tube defects. This study investigated MGMT mRNA expression and methylation levels in the early embryo and in different embryonic stages, as well as the relationship between MGMT and neural tube defects. Spina bifida aperta was induced in rats by a single intragastric administration of all-trans retinoic acid on embryonic day (E) 10, whereas normal control rats received the same amount of olive oil on the same embryonic day. DNA damage was assessed by detecting γ-H2A.X in spina bifida aperta rats. Real time-polymerase chain reaction was used to examine mRNA expression of MGMT in normal control and spina bifida aperta rats. In normal controls, the MGMT mRNA expression decreased with increasing embryonic days, and was remarkably reduced from E11 to E14, reaching a minimum at E18. In the spina bifida aperta model, γ-H2A.X protein expression was increased, and mRNA expression of MGMT was markedly decreased on E14, E16, and E18. Bisulfite sequencing polymerase chain reaction for MGMT promoter methylation demonstrated that almost all CpG sites in the MGMT promoter remained unmethylated in both spina bifida aperta rats and normal controls, and there was no significant difference in methylation level between the two groups on either E14 or E18. Our results show that DNA damage occurs in spina bifida aperta rats. The mRNA expression of MGMT is downregulated, and this downregulation is independent of promoter DNA methylation. 展开更多
关键词 nerve REGENERATION NEURAL tube defects spina bifida aperta spinal cord ALL-TRANS retinoic acid O6-methylguanine DNA methyltransferase gene expression DNA methylation PROMOTER BISULFITE sequencing polymerase chain reaction NEURAL REGENERATION
在线阅读 下载PDF
Impacts of Bioinoculants Pseudomonas jesenii MP1 and Rhodococcus qingshengii S10107 on Chickpea (Cicer arietinum L.) Yield and Soil Nitrogen St at us
2
作者 Divya JOSHI Ramesh CHANDRA +2 位作者 Deep Chandra SUYAL Saurabh KUMAR Reeta GOEL 《土壤圈:英文版》 SCIE CAS CSCD 2019年第3期388-399,共12页
Cold-adapted bioinoculants are considered as harbingers of sustainable hill agriculture. Therefore, two previously characterized psychrotolerant diazotrophs, Pseudomonas jesenii MP1 and Rhodococcus qingshengii S10107,... Cold-adapted bioinoculants are considered as harbingers of sustainable hill agriculture. Therefore, two previously characterized psychrotolerant diazotrophs, Pseudomonas jesenii MP1 and Rhodococcus qingshengii S10107, were evaluated for their plant growthpromoting potential for chickpea (Cicer arietinum L.) grown under natural field conditions. Comparative analysis of agronomical and biochemical crop parameters revealed the irrelevance of chemical fertilizers for chickpea production;the diazotrophs alone were sufficient to fulfil the crop's nutritional requirement. However, the integrated use of bacterial strains in combination with urea at 20 kg N ha^-1 as urea was being recommended for higher crop yield and better soil nitrogen status. Quantitative polymerase chain reaction (qPCR) and denaturing gradient gel electrophoresis (DGGE)-based soil bacterial dynamics unveiled the persistence of both diazotrophs until the end of the crop maturation period without affecting the native micro-flora. Therefore, these bioinoculants can be explored as natural nitrogen resource, and an additional incentive in their bio-formulation will be a step towards agricultural sustainability. 展开更多
关键词 agricultural sustainability denaturing gradient gel ELECTROPHORESIS (DGGE) HILL agriculture psychrotolerance N fertilizer N fixation N uptake quantitative polymerase chain reaction (qPCR)
Gene expression changes in dorsal root ganglia following peripheral nerve injury:roles in inflammation,cell death and nociception 预览
3
作者 Sarah L. Martin Adam J. Reid +2 位作者 Alexei Verkhratsky Valerio Magnaghi Alessandro Faroni 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期939-947,共9页
Subsequent to a peripheral nerve injury,there are changes in gene expression within the dorsal root ganglia in response to the damage.This review selects factors which are well-known to be vital for inflammation,cell ... Subsequent to a peripheral nerve injury,there are changes in gene expression within the dorsal root ganglia in response to the damage.This review selects factors which are well-known to be vital for inflammation,cell death and nociception,and highlights how alterations in their gene expression within the dorsal root ganglia can affect functional recovery.The majority of studies used polymerase chain reaction within animal models to analyse the dynamic changes following peripheral nerve injuries.This review aims to highlight the factors at the gene expression level that impede functional recovery and are hence are potential targets for therapeutic approaches.Where possible the experimental model,specific time-points and cellular location of expression levels are reported. 展开更多
关键词 Gene expression polymerase chain reaction dorsal root GANGLIA INFLAMMATION NOCICEPTION cell death peripheral NERVE injury Schwann CELLS satellite GLIAL CELLS NERVE regeneration
在线阅读 下载PDF
Atypical chemokine receptor CCRL2 is overexpressed in prostate cancer cells 预览
4
作者 Niradiz Reyes Ines Benedetti +2 位作者 Juan Rebollo Oscar Correa Jan Geliebter 《生物医学研究杂志:英文版》 CAS CSCD 2019年第1期17-23,共7页
Atypical chemokine receptors have recently emerged as important molecular players in health and diseases;they affect chemokine availability and function and impact a multitude of pathophysiological events,including th... Atypical chemokine receptors have recently emerged as important molecular players in health and diseases;they affect chemokine availability and function and impact a multitude of pathophysiological events,including the tumorigenesis process.This family of atypical receptors comprises five members:ACKR1/DARC,ACKR2/D6,ACKR3/CXCR7,ACKR4/CCRL1,and ACKR5/CCRL2.This work evaluated the differential expression of these receptors in prostate cancer using quantitative PCR.Further evaluation of CCRL2 at the protein level confirmed its overexpression in a metastatic cell line and in malignant prostatic tissues from patients.CCRL2,apresumed member of the atypical chemokine receptor family,plays akey role in lung dendritic cell trafficking to peripherally mphnodes.Recent studies have reported the expression of CCRL2 in different human cancer cell lines and tissues.However,its function and expression in prostate cancer has not been previously addressed. 展开更多
关键词 CHEMOKINE RECEPTOR PROSTATIC NEOPLASMS CCRL2 RECEPTOR real-time POLYMERASE chain reaction tissue array analysis
在线阅读 免费下载
Expression signatures of long non-coding RNA and mRNA in human traumatic brain injury 预览
5
作者 Li-Xiang Yang Li-Kun Yang +3 位作者 Jie Zhu Jun-Hui Chen Yu-Hai Wang Kun Xiong 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期632-641,共10页
Long non-coding RNAs(lncRNAs)play a key role in craniocerebral disease,although their expression profiles in human traumatic brain injury are still unclear.In this regard,in this study,we examined brain injury tissue ... Long non-coding RNAs(lncRNAs)play a key role in craniocerebral disease,although their expression profiles in human traumatic brain injury are still unclear.In this regard,in this study,we examined brain injury tissue from three patients of the 101st Hospital of the People’s Liberation Army,China(specifically,a 36-year-old male,a 52-year-old female,and a 49-year-old female),who were diagnosed with traumatic brain injury and underwent brain contusion removal surgery.Tissue surrounding the brain contusion in the three patients was used as control tissue to observe expression characteristics of lncRNAs and mRNAs in human traumatic brain injury tissue.Volcano plot filtering identified 99 lncRNAs and 63 mRNAs differentially expressed in frontotemporal tissue of the two groups(P<0.05,fold change>1.2).Microarray analysis showed that 43 lncRNAs were up-regulated and 56 lncRNAs were down-regulated.Meanwhile,59 mRNAs were up-regulated and 4 mRNAs were down-regulated.Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses revealed 27 signaling pathways associated with target genes and,in particular,legionellosis and influenza A signaling pathways.Subsequently,a lncRNA-gene network was generated,which showed an absolute correlation coefficient value>0.99 for 12 lncRNA-mRNA pairs.Finally,quantitative real-time polymerase chain reaction confirmed different expression of the five most up-regulated mRNAs within the two groups,which was consistent with the microarray results.In summary,our results show that expression profiles of mRNAs and lncRNAs are significantly different between human traumatic brain injury tissue and surrounding tissue,providing novel insight regarding lncRNAs’involvement in human traumatic brain injury.All participants provided informed consent.This research was registered in the Chinese Clinical Trial Registry(registration number:ChiCTR-TCC-13004002)and the protocol version number is 1.0. 展开更多
关键词 nerve REGENERATION HUMAN TRAUMATIC brain injuries long noncoding RNA messenger RNA GO ANALYSIS real-time quantitative polymerase chain reaction biomarkers microarray ANALYSIS biological processes medical informatics neural REGENERATION
在线阅读 下载PDF
Fibroblast growth factor receptor 4 single nucleotide polymorphism Gly388Arg in head and neck carcinomas 预览
6
作者 Eva Wimmer Stephan Ihrler +3 位作者 Olivier Gires Sylvia Streit Wolfgang Issing Christoph Bergmann 《世界临床肿瘤学杂志(英文版)》 2019年第3期136-148,共13页
BACKGROUND Head and neck squamous cell carcinoma(HNSCC) is considered to be a progressive disease resulting from alterations in multiple genes regulating cell proliferation and differentiation like receptor tyrosine k... BACKGROUND Head and neck squamous cell carcinoma(HNSCC) is considered to be a progressive disease resulting from alterations in multiple genes regulating cell proliferation and differentiation like receptor tyrosine kinases(RTKs) and members of the fibroblast growth factor receptors(FGFR)-family. Singlenucleotide polymorphism(SNP) Arg388 of the FGFR4 is associated with a reduced overall survival in patients with cancers of various types. We speculate that FGFR4 expression and SNP is associated with worse survival in patients with HSNCC.AIM To investigate the potential clinical significance of FGFR4 Arg388 in the context of tumors arising in HNSCC, a comprehensive analysis of FGFR4 receptor expression and genotype in tumor tissues and correlated results with patients’ clinical data in a large cohort of patients with HNSCC was conducted.METHODS Surgical specimens from 284 patients with HNSCC were retrieved from the Institute of Pathology at the Ludwig-Maximilian-University in Germany.Specimens were analyzed using immunohistochemistry and polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). The expression of FGFR4 was analyzed in 284 surgical specimens of HNSCC using immunohistochemstry. FGFR4 polymorphism was detected by PCR-RFLP.Patients’ clinical data with a minimum follow-up of 5 years were statistically evaluated with a special emphasis on survival analysis employing Kaplan-Meier estimator and Cox regression analysis.RESULTS Concerning the invasive tumor areas the intensity of the FGFR4 expression was evaluated in a four-grade system: no expression, low expression, intermediate and high expression. FGFR4 expression was scored as 'high'(+++) in 74(26%),'intermediate'(++) in 103(36.3%), and 'low'(+) in 107(36.7%) cases. Analyzing the FGFR4 mutation it was found in 96 tumors(33.8%), 84 of them(29.6%) having a heterozygous and 12(4.2%) homozygous mutated Arg388 allele. The overall frequency concerning the mutant alleles demonstrated 65% vs 34% mutated alleles in general. FGFR4 Arg388 wa 展开更多
关键词 FIBROBLAST growth factor receptor 4 Single-nucleotide polymorphism Head and NECK SQUAMOUS cell carcinoma Reduced survival Cancer progression POLYMERASE chain reaction IMMUNOHISTOCHEMISTRY Outcome
在线阅读 免费下载
Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus
7
作者 Ryong Gong Dae Young Lee +4 位作者 Jae Won Lee Doo Jin Choi Geum-Soog Kim Sang Hyuk Lee Young-Seob Lee 《亚太热带医药杂志:英文版》 SCIE CAS 2019年第1期14-18,共5页
Objective: To investigate the synergistic effect between rhein(RHE) and oxacillin against Staphylococcus aureus(MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were c... Objective: To investigate the synergistic effect between rhein(RHE) and oxacillin against Staphylococcus aureus(MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/blaR1 gene expressions. 展开更多
关键词 Antimicrobials Mechanism of action STAPHYLOCOCCUS AUREUS POLYMERASE CHAIN reaction
The D253N Mutation in the Polymerase Basic 2 Gene in Avian Influenza(H9N2) Virus Contributes to the Pathogenesis of the Virus in Mammalian Hosts
8
作者 Jinfeng Zhang Rong Su +3 位作者 Xiaoyun Jian Hongliang An Ronbing Jiang Chris Ka Pun Mok 《中国病毒学:英文版》 CAS CSCD 2018年第6期531-537,共7页
Mutations in the polymerase basic 2(PB2) gene of avian influenza viruses are important signatures for their adaptation to mammalian hosts. Various adaptive mutations have been identified around the 627 and nuclear loc... Mutations in the polymerase basic 2(PB2) gene of avian influenza viruses are important signatures for their adaptation to mammalian hosts. Various adaptive mutations have been identified around the 627 and nuclear localization sequence(NLS) domains of PB2 protein, and these mutations contribute to the replicative ability of avian influenza viruses.However, few studies have focused on adaptive mutations in other regions of PB2. In this study, we investigated the functional roles of the D253N mutation in PB2 in an H9N2 virus. This mutation was found to affect an amino acid residue in the middle domain of the PB2 protein. The virus with the D253N mutation showed higher polymerase activity and transiently increased viral replication in human cells. However, the mutant did not show significant differences in viral replication in the respiratory tract of mice upon infection. Our results supported that the D253N mutation in the middle domain of PB2, similar to mutations at the 627 and NLS domains, specifically contributed to the replication of avian influenza viruses in human cells. 展开更多
关键词 AVIAN influenza VIRUS MAMMALIAN adaptation D253N POLYMERASE basic 2(PB2) H9N2
Naturally occurring hepatitis B virus reverse transcriptase mutations related to potential antiviral drug resistance and liver disease progression 预览
9
作者 Yu-Min Choi So-Young Lee Bum-Joon Kim 《世界胃肠病学杂志:英文版》 SCIE CAS 2018年第16期1708-1724,共17页
The annual number of deaths caused by hepatitis B virus(HBV)-related disease,including cirrhosis and hepatocellular carcinoma(HCC),is estimated as 887000.The reported prevalence of HBV reverse transcriptase(RT)mutatio... The annual number of deaths caused by hepatitis B virus(HBV)-related disease,including cirrhosis and hepatocellular carcinoma(HCC),is estimated as 887000.The reported prevalence of HBV reverse transcriptase(RT)mutation prior to treatment is varied and the impact of preexisting mutations on the treatment of na?ve patients remains controversial,and primarily depends on geographic factors,HBV genotypes,HBeAg serostatus,HBV viral loads,disease progression,intergenotypic recombination and co-infection with HIV.Different sensitivity of detection methodology used could also affect their prevalence results.Several genotype-dependent HBV RT positions that can affect the emergence of drug resistance have also been reported.Eight mutations in RT(rtL80I,rtD134N,rtN139K/T/H,rtY141F,rtM204I/V,rtF221Y,rtI224V,and rtM309K)are significantly associated with HCC progression.HBeAg-negative status,low viral load,and genotype C infection are significantly related to a higher frequency and prevalence of preexisting RT mutations.Preexisting mutations are most frequently found in the A-B interdomain of RT which overlaps with the HBsAg“a”determinant region,mutations of which can lead to simultaneous viral immune escape.In conclusion,the presence of baseline RT mutations can affect drug treatment outcomes and disease progression in HBVinfected populations via modulation of viral fitness and host-immune responses. 展开更多
关键词 POLYMERASE HEPATOCELLULAR carcinoma REVERSE transcriptase preexisting MUTATIONS hepatitis B virus
在线阅读 免费下载
长链非编码RNA AC023794.4-201在喉鳞状细胞癌中的表达及临床意义
10
作者 童巧玲 沈志森 +2 位作者 李群 郝文娟 周重昌 《中国耳鼻咽喉头颈外科》 CSCD 2018年第10期564-566,共3页
喉癌是头颈部最常见的恶性肿瘤之一,其中85%~95%为鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)[1]。据报道早期LSCC患者的生存率远高于晚期患者[2]。研究发现,长链非编码RNA(long non-coding RNA,lncRNA)参与调控头颈部... 喉癌是头颈部最常见的恶性肿瘤之一,其中85%~95%为鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)[1]。据报道早期LSCC患者的生存率远高于晚期患者[2]。研究发现,长链非编码RNA(long non-coding RNA,lncRNA)参与调控头颈部肿瘤细胞的增殖、分化、迁移和侵袭等,发挥抑癌基因或癌基因的功能[3~5]。 展开更多
关键词 喉肿瘤(Laryngeal Neoplasms) 鳞状细胞(Carcinoma Squamous Cell) 逆转录聚合酶链反应(Reverse Transcriptase POLYMERASE Chain Reaction) 预后(Prognosis) 长链非编码RNA(long NON-CODING RNA)
Diversity of dsRNA Viruses Infecting Rice Sheath Blight Fungus Rhizoctonia solani AG-1 IA 预览
11
作者 ZHENG Li LIU Chen +2 位作者 ZHANG Meiling YANG Mei ZHOU Erxun 《水稻科学:英文版》 CSCD 2018年第1期57-60,共4页
Rice sheath blight, caused by Rhizoctonia solani (Kühn), is a notorious soil-borne disease prevalent in many rice-growing regions. Although several sporadic studies of mycoviruses in R. solani AG-1 IA have been r... Rice sheath blight, caused by Rhizoctonia solani (Kühn), is a notorious soil-borne disease prevalent in many rice-growing regions. Although several sporadic studies of mycoviruses in R. solani AG-1 IA have been reported for single strain of R. solani AG-1 IA, there have been no reports describing the distribution and diversity of mycoviruses in natural populations. In this study, 43 R. solani AG-1 IA strains collected from different locations in China were examined for the presence of dsRNA elements to confirm the presence of viral infections. Electrophoretypes showed that 16 of the 43 fungal strains (37.2%) contained dsRNAs that can be characterized as viruses. Furthermore, the species-specific reverse transcription PCR (RT-PCR) showed dsRNA bands with similar sizes do not always contain the same virus but exist as mixed mycoviral infections.Thus, our findings indicate mycoviruses infecting R. solani AG-1 IA in China are diverse, widespread and universal. 展开更多
关键词 MYCOVIRUS RHIZOCTONIA SOLANI reverse transcription polymerase chain reaction DIVERSITY dsRNA rice SHEATH blight
在线阅读 下载PDF
Differential expression of mucin 1 and mucin 2 in colorectal cancer 预览
12
作者 Aldona Kasprzak Elzbieta Siodla +4 位作者 Malgorzata Andrzejewska Jacek Szmeja Agnieszka Seraszek-Jaros Szczepan Cofta Witold Szaflarski 《世界胃肠病学杂志:英文版》 SCIE CAS 2018年第36期4164-4177,共14页
AIM To determine tissue expression(mRNA,protein)of two types of mucins[mucin 1(MUC1)and mucin 2(MUC2)]in patients with colorectal cancer(CRC).METHODS Expression of membrane-bound mucin(MUC1)and secretory mucin(MUC2)in... AIM To determine tissue expression(mRNA,protein)of two types of mucins[mucin 1(MUC1)and mucin 2(MUC2)]in patients with colorectal cancer(CRC).METHODS Expression of membrane-bound mucin(MUC1)and secretory mucin(MUC2)in CRC(mRNA,protein)were analyzed in tissue material including fragments of tumors obtained from CRC patients(n=34),and fragments of normal colorectal tissue from the same patients(control).The analysis was conducted using real-time quantitative polymerase chain reaction(RT-qPCR)(transcripts),immunohistochemistry(IHC)(apomucins),and the modern approach for morphometric analysis of IHC reaction(HSV filter software).Results on tissue expression of both mucins(mRNA,protein)were compared to histological alterations in colorectal cancer samples and correlated with selected clinical data in the patients.The statistical analysis was conducted using Statistica PL v.12.0 software.RESULTS Significantly higher expression of the MUC1 mRNA in the CRC,compared with the control and the borderline correlation of mRNA expression with MUC1 protein levels in colorectal samples was observed.The expression of apomucins concerned cell membranes(MUC1)and cytoplasm(MUC2)and occurred both in control tissues and in most cancerous samples.There were no significant relationships between MUC1(mRNA,protein)and the clinicopathological data of patients.MUC2 protein expression was significantly lower as compared to the control,while MUC2 mRNA expression was comparable in both groups.The MUC1/MUC2 ratio was significantly higher in CRC tissues than in the control.The higher expression of MUC2 was a feature of mucinous CRC subtypes,and characterized higher histological stage of tumors.Negative correlations have been obtained between MUC2 and the Ki-67 antigen,as well as between MUC2 and p53 protein expressions in CRC.CONCLUSION A combination of tissue overexpression of MUC1,reduced MUC2 expression,and high ratio of MUC1/MUC2 is a factor of poor prognosis in CRC patients.MUC2 tissue expression allows to differentiate mucinous and nonmucin 展开更多
关键词 MUCINS Real-time quantitative POLYMERASE chain reaction Colorectal cancer IMMUNOHISTOCHEMISTRY HSV filter program
在线阅读 免费下载
以色列急性麻痹病毒(IAPV)RT-PCR检测方法的建立及应用
13
作者 张体银 李丹丹 +4 位作者 郑腾 白泉阳 张志灯 王武军 于师宇 《中国兽医学报》 CSCD 北大核心 2018年第6期1105-1108,共4页
为建立以色列急性麻痹病毒(Israeli acute paralysis virus,IAPV)特异性核酸检测方法,本研究以IAPV polvmerase polyprotein基因为靶基因设计合成1对特异性引物,建立了IAPV的RTPCR检测方法。结果显示,对蜜蜂的5种不同病毒进行RT-... 为建立以色列急性麻痹病毒(Israeli acute paralysis virus,IAPV)特异性核酸检测方法,本研究以IAPV polvmerase polyprotein基因为靶基因设计合成1对特异性引物,建立了IAPV的RTPCR检测方法。结果显示,对蜜蜂的5种不同病毒进行RT-PCR检测,只有IAPV病毒检测结果为阳性,具有良好的特异性;对IAPV的最低检测限为10^3拷贝/μL,具有较高的灵敏度;利用该检测方法对来自福建省不同养蜂场的8份蜜蜂样品进行检测,3份样品为阳性,通过序列比对分析,同源性超过99%,进一步证实为IAPV。结果表明,所建立的IAPV RT-PCR检测方法灵敏度高、特异性强、重复性好,具有良好的实用性。 展开更多
关键词 以色列急性麻痹病毒 RT-PCR POLYMERASE polyprotein基因 蜜蜂
抗耐药疱疹病毒药物的研究进展
14
作者 黄岭 张伊萌 +2 位作者 张晓花 李剑 宋金春 《中国药师》 CAS 2017年第7期1289-1292,共4页
疱疹病毒是人类最常见的感染性疾病,根据人类感染程度可分为不同种类,其中单纯疱疹病毒通常引起人类口腔或生殖器黏膜感染,巨细胞病毒是器官移植和肿瘤患者等免疫功能不全患者最常见机会致病菌,可导致严重的疾病甚至死亡。核苷类似物是... 疱疹病毒是人类最常见的感染性疾病,根据人类感染程度可分为不同种类,其中单纯疱疹病毒通常引起人类口腔或生殖器黏膜感染,巨细胞病毒是器官移植和肿瘤患者等免疫功能不全患者最常见机会致病菌,可导致严重的疾病甚至死亡。核苷类似物是治疗疱疹病毒疗效较好的药物,但耐药病毒株的出现,为疱疹病毒感染患者的治疗设置了新的障碍,尤其对于免疫功能不全的患者。因此临床亟需能够规避疱疹病毒耐药性的有效的抗病毒药物。本文综述了耐药疱疹病毒的治疗药物的研究进展,包括非核苷类抗病毒药物、新型作用靶点、新型作用机制的抗病毒药物的研究进展。 展开更多
关键词 疱疹病毒 耐药 聚合酶 新型作用靶点
Real-world cure rates for hepatitis C virus treatments that include simeprevir and/or sofosbuvir are comparable to clinical trial results 预览
15
作者 Kian Bichoupan Neeta Tandon +20 位作者 James F Crismale Joshua Hartman David Del Bello Neal Patel Sweta Chekuri Alyson Harty Michel Ng Keith M Sigel Meena B Bansal Priya Grewal Charissa Y Chang Jennifer Leong Gene Y Im Lawrence U Liu Joseph A Odin Nancy Bach Scott L Friedman Thomas D Schiano Ponni V Perumalswami Douglas T Dieterich Andrea D Branch 《世界病毒学杂志》 2017年第4期59-72,共14页
AIM To assess the real-world effectiveness and cost of simeprevir(SMV),and/or sofosbuvir(SOF)-based therapy for chronic hepatitis C virus(HCV)infection.METHODS The real-world performance of patients treated with SMV/S... AIM To assess the real-world effectiveness and cost of simeprevir(SMV),and/or sofosbuvir(SOF)-based therapy for chronic hepatitis C virus(HCV)infection.METHODS The real-world performance of patients treated with SMV/SOF±ribavirin(RBV),SOF/RBV,and SOF/RBV with pegylated-interferon(PEG)were analyzed in a consecutive series of 508 patients with chronic HCV infection treated at a single academic medical center.Patients with genotypes 1 through 4 were included.Rates of sustained virological response-the absence of a detectable serum HCV RNA 12 wk after the end of treatment[sustained virological response(SVR)12]-were calculated on an intention-to-treat basis.Costs were calculated from the payer’s perspective using Medicare/Medicaid fees and Redbook Wholesale Acquisition Costs.Patient-related factors associated with SVR12 were identified using multivariable logistic regression.SVR12 rates were as follows:86%(95%CI:80%-91%)among 178 patients on SMV/SOF±RBV;62%(95%CI:55%-68%)among 234 patients on SOF/RBV;and 78%(95%CI:68%-86%)among 96 patients on SOF/PEG/RBV.Mean costs-per-SVR12 were$174442(standard deviation:±$18588)for SMV/SOF±RBV;$223003(±$77946)for SOF/RBV;and$126496(±$31052)for SOF/PEG/RBV.Among patients on SMV/SOF±RBV,SVR12 was less likely in patients previously treated with a protease inhibitor[odds ratio(OR):0.20,95%CI:0.06-0.56].Higher bilirubin(OR:0.47,95%CI:0.30-0.69)reduced the likelihood of SVR12 among patients on SOF/RBV,while FIB-4 score≥3.25 reduced the likelihood of SVR12(OR:0.18,95%CI:0.05-0.59)among those on SOF/PEG/RBV.CONCLUSION SVR12 rates for SMV and/or SOF-based regimens in a diverse real-world population are comparable to those in clinical trials.Treatment failure accounts for 27%of costs. 展开更多
关键词 Cirrhosis Cost SUSTAINED virological response PROTEASE INHIBITOR Polymerase INHIBITOR
在线阅读 免费下载
DNA的滚环扩增合成研究 预览
16
作者 刘淑贞 张志庆 +5 位作者 王芳 周亭 王秀凤 张国栋 刘婷婷 张洪芝 《物理化学学报》 CSCD 北大核心 2017年第10期2052-2057,共6页
滚环扩增(RCA)反应作为一种简单高效的等温酶促反应,现己发展为核酸扩增领域的新技术,其产物在组装体搭建和多功能材料的制备方面有着广泛的应用。本文采用琼脂糖凝胶、紫外和透射电镜(TEM)等手段,探究了时间、三磷酸脱氧核糖核... 滚环扩增(RCA)反应作为一种简单高效的等温酶促反应,现己发展为核酸扩增领域的新技术,其产物在组装体搭建和多功能材料的制备方面有着广泛的应用。本文采用琼脂糖凝胶、紫外和透射电镜(TEM)等手段,探究了时间、三磷酸脱氧核糖核苷(dNTPs)、酶以及引物的浓度等因素对脱氧核糖核酸(DNA)滚环扩增产物的影响。结果表明:在反应开始的前30min,RCA产物的长度受时间的影响比较明显;随着dNTPs浓度的提高,RCA产物的链长增长,浓度也不断提高;酶和引物的浓度对滚环扩增产物的长度没有明显影响,但对RCA产物浓度的影响较大,过量的酶致使RCA产物的含量显著下降。 展开更多
关键词 滚环扩增技术 核酸 dNTPs 聚合酶 引物
在线阅读 下载PDF
通过自诱导方法对乙型肝炎病毒聚合酶TP区进行可溶性表达及鉴定 被引量:4
17
作者 陈可 代娟 +4 位作者 甘春扬 刘亚 罗英英 胡接力 蔡雪飞 《中国生物制品学杂志》 CAS CSCD 2017年第1期25-28,33共5页
目的构建包含乙型肝炎病毒(hepatitis B virus,HBV)聚合酶TP区段的重组原核表达质粒,转化表达型大肠埃希菌,以自诱导培养方法获得可溶性表达,并对其进行鉴定。方法分析HBV(A型)聚合酶N-末端1-192 AA区域的DNA序列,通过网络工具(htt... 目的构建包含乙型肝炎病毒(hepatitis B virus,HBV)聚合酶TP区段的重组原核表达质粒,转化表达型大肠埃希菌,以自诱导培养方法获得可溶性表达,并对其进行鉴定。方法分析HBV(A型)聚合酶N-末端1-192 AA区域的DNA序列,通过网络工具(http://www.jcat.de/)在线优化,并在5′和3′端分别添加NdeⅠ和XhoⅠ限制性内切酶位点后,送英潍捷基(上海)贸易有限公司进行人工合成;将人工合成的TP-DNA双酶切后,插入pET32a(+)原核表达载体,构建重组原核表达质粒,转化大肠埃希菌BL21(DE3)pLysS进行自诱导表达。结果重组原核表达质粒pET32a(+)/POL-TP-Opt经双酶切及测序证明构建正确;在表达菌的破菌上清中有特异性表达的蛋白,相对分子质量约20 000,经SDS-PAGE和Western blot鉴定,为可溶性的重组TP蛋白。结论通过自诱导培养方法,直接成功获得可溶性的重组TP蛋白,改变了长期以来依靠包涵体复性对TP蛋白相关功能进行研究的状况。 展开更多
关键词 自诱导 乙型肝炎病毒 聚合酶 TP区 原核细胞 基因表达
Naturally occurring mutations in the reverse transcriptase region of hepatitis B virus polymerase from treatment-na?ve Korean patients infected with genotype C2 预览
18
作者 Ji-Eun Kim So-Young Lee +3 位作者 Hong Kim Ki-Jeong Kim Won-Hyeok Choe Bum-Joon Kim 《世界胃肠病学杂志:英文版》 SCIE CAS 2017年第23期4222-4232,共11页
AIM To report naturally occurring mutations in the reverse transcriptase region(RT) of hepatitis B virus(HBV) polymerase from treatment na?ve Korean chronic patients infected with genotype C2.METHODS Here, full-length... AIM To report naturally occurring mutations in the reverse transcriptase region(RT) of hepatitis B virus(HBV) polymerase from treatment na?ve Korean chronic patients infected with genotype C2.METHODS Here, full-length HBV reverse transcriptase RT sequences were amplified and sequenced from 131 treatment na?ve Korean patients chronically infected with hepatitis B genotype C2. The patients had two distinct clinical statuses: 59 patients with chronic hepatitis(CH) and 72 patients with hepatocellular carcinoma(HCC). The deduced amino acids(AAs) at42 previously reported potential nucleos(t)ide analog resistance(NAr) mutation positions in the RT region were analyzed. RESULTS Potential NAr mutations involving 24 positions were found in 79 of the 131 patients(60.3%). Notably, AA substitutions at 2 positions(rt184 and rt204) involved in primary drug resistance and at 2 positions(rt80 and rt180) that functioned as secondary/compensatory mutations were detected in 10 patients(1 CH patient and 9 HCC patients) and 7 patients(1 CH and 6 HCC patients), respectively. The overall mutation frequencies in the HCC patients(3.17%, 96/3024 mutations) were significantly higher than the frequencies in the CH patients(2.09%, 52/2478 mutations)(P = 0.003). In addition, a total of 3 NAr positions, rt80, rt139 and rt204 were found to be significantly related to HCC from treatment na?ve Korean patients. CONCLUSION Our data showed that naturally occurring NAr mutations in South Korea might contribute to liver disease progression(particularly HCC generation) in chronic patients with genotype C2 infections. 展开更多
在线阅读 免费下载
Molecular cloning, characterization, and promoter analysis of the isochorismate synthase (AalCS1) gene from Artemisia annua
19
作者 Lu-yao WANG Ying ZHANG +7 位作者 Xue-qing FU Ting-ting ZHANG Jia-wei MA Li-da ZHANG Hong-mei QIAN Ke-xuan TANG Shan LI Jing-ya ZHAO 《浙江大学学报:B卷英文版》 SCIE CAS CSCD 2017年第8期662-673,共12页
Isochorismate synthase (ICS) is a crucial enzyme in the salicylic acid (SA) synthesis pathway. Thefull-length complementary DNA (cDNA) sequence of the ICS gene was isolated from Artemisia annua L. The gene,named... Isochorismate synthase (ICS) is a crucial enzyme in the salicylic acid (SA) synthesis pathway. Thefull-length complementary DNA (cDNA) sequence of the ICS gene was isolated from Artemisia annua L. The gene,named AalCS1, contained a 1710-bp open reading frame, which encoded a protein with 570 amino acids. Bioinfor-matics and comparative study revealed that the polypeptide protein of AalCS1 had high homology with ICSs from otherplant species. Southern blot analysis suggested that AalCS1 might be a single-copy gene. Analysis of the 1470-bppromoter of AalCS1 identified distinct cis-acting regulatory elements, including TC-rich repeats, MYB binding site(MBS), and TCA-elements. An analysis of AalCS1 transcript levels in multifarious tissues of A. annua using quantita-tive real-time polymerase chain reaction (qRT-PCR) showed that old leaves had the highest transcription levels.AalCS1 was up-regulated under wounding, drought, salinity, and SA treatments. This was corroborated by thepresence of the predicted cis-acting elements in the promoter region of AalCSI. Overexpressing transgenic plants andRNA interference transgenic lines of AalCSl were generated and their expression was compared. High-performanceliquid chromatography (HPLC) results from leaf tissue of transgenic A. annua showed an increase in artemisinincontent in the overexpressing plants. These results confirm that AalCS1 is involved in the isochorismate pathway. 展开更多
关键词 Salicylic acid ARTEMISIA annua L. QUANTITATIVE real-time POLYMERASE chain reaction (qRT-PCR) Isochorismate SYNTHASE
核因子蛋白90敲减对肝癌细胞增殖影响及机制的研究 预览
20
作者 宋丹 魏莉 吴家雪 《临床肿瘤学杂志》 CAS 2017年第5期385-390,共6页
目的 探究核因子蛋白90(NF90)敲减对肝癌细胞增殖影响并探讨其可能机制.方法 将靶向NF90的寡核苷酸链克隆至PLKO质粒后,包装出靶向敲减NF90的慢病毒shRNA(带有绿色荧光标签及G418抗性).将肝癌细胞QGY-7703、SMMC-7721分为对照组及... 目的 探究核因子蛋白90(NF90)敲减对肝癌细胞增殖影响并探讨其可能机制.方法 将靶向NF90的寡核苷酸链克隆至PLKO质粒后,包装出靶向敲减NF90的慢病毒shRNA(带有绿色荧光标签及G418抗性).将肝癌细胞QGY-7703、SMMC-7721分为对照组及干扰组,分别感染包含随机序列shRNA和靶向NF90 shRNA的病毒液.48 h后流式分选出带有绿色荧光的阳性细胞,G418筛选阳性单克隆细胞株,Western blotting鉴定NF90的蛋白表达水平,最终在对照组中选取一株细胞命名shRNA-ns,在干扰组中选取NF90敲减效果良好且稳定的两株细胞命名为shRNA-1、shRNA-2.采用CCK-8法检测各株细胞的增殖情况,克隆形成试验分析各株细胞的克隆形成能力.质谱分析预测NF90的相关结合蛋白,内、外源免疫共沉淀确定NF90与多聚ADP核糖合成酶(PARP1)的关系,荧光定位分析NF90与PARP1在细胞内的定位情况.结果 shRNA-1、shRNA-2细胞中的NF90表达水平显著低于shRNA-ns细胞,表明包装的慢病毒敲减NF90效果良好.与shRNA-ns细胞比较,shRNA-1、shRNA-2细胞的生长缓慢,克隆形成数减少,差异均有统计学意义(P〈0.01).NF90与PARP1在细胞内相互结合并且共定位于细胞核.结论 敲减NF90可抑制肝癌细胞增殖,其机制可能与PARP1相互作用有关. 展开更多
关键词 肝细胞癌 核因子蛋白90 多聚ADP核糖合成酶 细胞增殖 NUCLEAR FACTOR 90 POLY (ADP-ribose) POLYMERASE 1
在线阅读 下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部 意见反馈