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噻虫嗪处理的芫菁夜蛾斯氏线虫SF-SN对韭菜迟眼蕈蚊幼虫的杀虫效果和搜寻效应 预览
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作者 武海斌 宫庆涛 +4 位作者 陈珍珍 范昆 公义 许永玉 孙瑞红 《昆虫学报》 CAS CSCD 北大核心 2019年第2期222-232,共11页
【目的】昆虫病原线虫受到化学农药作用后,其对害虫的杀虫效果和搜寻效应可能会发生变化。本研究旨在探讨噻虫嗪处理的昆虫病原线虫对韭菜迟眼蕈蚊 Bradysia odoriphaga 幼虫(韭蛆)的杀虫效果及搜寻效应的影响。【方法】采用培养皿滤纸... 【目的】昆虫病原线虫受到化学农药作用后,其对害虫的杀虫效果和搜寻效应可能会发生变化。本研究旨在探讨噻虫嗪处理的昆虫病原线虫对韭菜迟眼蕈蚊 Bradysia odoriphaga 幼虫(韭蛆)的杀虫效果及搜寻效应的影响。【方法】采用培养皿滤纸法,通过功能反应试验测定了噻虫嗪(15 mg/L)处理的芫菁夜蛾斯氏线虫 Steinernema feltiae SF-SN (Sf)对韭蛆3龄幼虫的致死率和搜寻效应,比较噻虫嗪处理的Sf和未处理的Sf致死功能反应和搜寻效应的差异。【结果】噻虫嗪处理的Sf引起的韭蛆3龄幼虫的校正死亡率高于未处理的Sf引起的校正死亡率,处理6 h时,较未处理的Sf引起的校正死亡率提高了2.13倍。当Sf浓度固定在6 400 IJs/皿时,噻虫嗪处理的和未处理的Sf对该试虫功能反应均拟合Holling Ⅱ和Ⅲ型方程,与未处理的Sf相比,噻虫嗪处理的Sf对该试虫的攻击率( a′=0.5592)提高了42.46%,线虫寻找、寄生及致死该试虫所花费的总时间即处理时间( Th =0.0081 d)则降低了44.90%,消耗率( a′/Th )提高了2.59倍,日最大致死量( Namax )则分别提高了1.81倍(Holling Ⅱ)和1.41倍(Holling Ⅲ)。而噻虫嗪处理的和未处理的Sf对该试虫的搜寻效应均随韭蛆密度的增加而呈线性下降。当韭蛆密度固定在40头/皿时,噻虫嗪处理的和未处理的Sf对该试虫的致死效果均随着韭蛆密度的增加而增大,搜寻效应则均先上升后下降,且噻虫嗪处理的Sf的寻找参数和相互干扰参数均高于未处理Sf的。【结论】噻虫嗪处理的Sf对韭蛆3龄幼虫的校正死亡率、瞬时攻击率、消耗率、日最大致死量和搜寻效应均高于未处理Sf的,而处理时间则显著降低。 展开更多
关键词 芫菁夜蛾斯氏线虫SF-SN 噻虫嗪 韭菜迟眼蕈蚊 杀虫效果 搜寻效应
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Comparative study on production,purification of penicillin by Penicillium chrysogenum isolated from soil and citrus samples
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作者 S Anto Jeya Dayalan Pramod Darwin Prakash S 《亚太热带生物医学杂志:英文版》 2011年第1期15-19,共5页
Objective:To explore various unexplored locations where Penicillium spp.would be available and study the production of penicillin from the isolated Penicillium spp.in different media with altered carbohydrate source.... Objective:To explore various unexplored locations where Penicillium spp.would be available and study the production of penicillin from the isolated Penicillium spp.in different media with altered carbohydrate source.Methods:The collected soil samples were screened for the isolation of Penicillium chrysogenum(P.chrysogenum) by soil dilution plate.The isolated Penicillium species were further grown in different production media with changes in the carbohydrate source.The extracted penicillin from various isolates was analyzed by HPLC for the efficacy of the product.Further the products were screened with various bacterial species including methicillin resistant Staphylococcus aureus(MRSA).And the work was extended to find the possible action on MRSA,along with characterization using other pathogens.Results:From the various soil and citrus samples used for analysis,only the soil sample from Government General Hospital of Bangalore,India,and Sanjay Gandhi Hospital,Bangalore,India,showed some potential growth of the desired fungi P.chrysogenum.Different production media showed varied range of growth of PenicilUum.Optimum production of penicillin was obtained in maltose which proved maximum zone of inhibition during assay.Characterization of penicillin on pathogens,like wild Escherichia coli strain,Klebsiella spp.,and MRSA,gave quite interesting results such as no activity on the later strain as it is resistant.HPLC data provided the analytical and confirmation details of the penicillin produced.Accordingly,the penicillin produced from the soil sample of Government General Hospital had the high milli absorbance unit of 441.5 mAu compared with that of the penicillin produced from Sanjay Gandhi Hospital sample,8S.S2 mAu.Therefore,there was a considerable change in quantity of the penicillin produced from both the samples.Conclusions: The Penicillium spp.could be possibly rich in hospital contaminants and its environments.This research focuses on various unexplored sources of medical ailments,and also shows that the grow 展开更多
关键词 PENICILLIUM chrysogenum SOIL CITRUS PENICILLIN Bioefficacy Antibiotic activity PENICILLIUM spp. PRODUCTION SOIL dilution plate Staphy lococcus aureus Characterization PRODUCTION media Zone of inhibition CARBOHYDRATE source Isolation Efficacy Pathogen
Bioefficacy of Mentha piperita essential oil against dengue fever mosquito Aedes aegypti L 被引量:4
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作者 Sarita Kumar Naim Wahab Radhika Warikoo 《亚太热带生物医学杂志:英文版》 CAS 2011年第2期85-88,共4页
Objective:To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant,Mentha piperita(M.piperita)against the larval and adult stages of Aedes aegypli(Ae.Aegy... Objective:To assess the larvicidal and repellent potential of the essential oil extracted from the leaves of peppermint plant,Mentha piperita(M.piperita)against the larval and adult stages of Aedes aegypli(Ae.Aegypti).Methods:The larvicidal potential of peppermint oil was evaluated against early fourth instar larvae of Ae.aegypli using WHO protocol.The mortality counts were made after 24 and 48 h,and LC<sub>50</sub>and LC<sub>90</sub>values were calculated.The efficacy of peppermint oil as mosquito repellent was assessed using the human-bait technique.The measured area of one arm of a human volunteer was applied with the oil and the other arm was applied with ethanol.The mosquito bites on both the arms were recorded for 3 rain after every 15 min.The experiment continued for 3 h and the percent protection was calculated.Results:The essential oil extracted from M.piperita possessed excellent larvicidal efficiency against dengue vector.The bioassays showed an LC<sub>50</sub>and LC<sub>90</sub>value of 111.9 and 295.18 ppm,respectively after 24 h of exposure.The toxicity of the oil increased 11.8%when the larvae were exposed to the oil for 48h.The remarkable repellent properties of M.piperita essential oil were established against adults Ae.aegypti.The application of oil resulted in 100%protection till 150 min.After next 30 min,only 1-2 bites were recorded as compared with 8-9 bites on the control arm.Conclusions:The peppermint essential oil is proved to be efficient larvicide and repellent against dengue vector.Further studies are needed to identify the possible role of oil as adulticide.oviposition deterrent and ovicidal agent.The isolation of active ingredient from the oil could help in formulating strategies for mosquito control. 展开更多
关键词 Essential oil LARVICIDE REPELLENT %protection Bioefficacy MENTHA piperita DENGUE AEDES aegypti LARVICIDAL potential Adulticide
In vivo and in vitro phytochemical and antibacterial efficacy of Baliospermum montanum (Willd.) Muell.Arg. 被引量:4
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作者 Johnson M Wesely EG +1 位作者 Zahir Hussain MI Selvan N 《亚太热带医药杂志:英文版》 SCIE 2010年第11期894-897,共4页
【正】Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in ... 【正】Objective:To evaluate the phytochemical and anti-bacterial potential of mother plants in vivo and in vitro derived callus of Baliospermum montanum(B.montanum)(Willd.) Muell.-Arg. leaves and root.Methods:The in vitro derived rootlets and leaves segments of B.montanum were cut into 0.5-0.7 cm in length and cultured on Murashige and Skoog solid medium supplemented with 3%sucrose,gelled with 0.7%agar and different concentration of 2,4-D either alone or in combinations.The preliminary phytochemical screening was performed by Harborne method.Antibacterial efficacy was performed by well diffusion method and incubated for 24 h at 37℃.Results:The highest percentage of callus formation(leaves segments 86.9±0.56:root segments 78.7±0.51) was obtained on Murashige and Skoog’s basal medium supplemented with 3%sucrose and 2.0 mg/L of 2.4-Dichlorophenoxy acetic acid.The phytochemical study revealed the high quantity presence of steroids,triterpenoids,glycosides,saponins,alkaloids,flavanoids, phenolic compounds,tannins,sugars etc of root and leaves derived calli.The ethanol extract of leaves segment derived calli of B.montanum showed the maximum solubility and antimicrobial activity with the MIC ranged from 100 to 200μL.Conclusions:The preliminary phytochemical study confirmed that the calli mediated tissues showed the higher percentage of metabolite constituents and extraction value compared to the in vivo leaves and roots.The present study observation suggested that a possibility to establish high yielding genotypes by in vitro culture for production of medicinally important bioactive compounds. 展开更多
关键词 CALLUS Bioefficacy Baliospermum montanum PHYTOCHEMICAL
DL-羟基蛋氨酸钙相对于DL-蛋氨酸的生物学效价 预览 被引量:3
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作者 李博 武书庚 +2 位作者 张海军 岳洪源 齐广海 《中国畜牧杂志》 CAS 北大核心 2010年第15期 35-40,共6页
试验采用肉仔鸡生产性能和胴体品质指标结合回归模型比较了DL-羟基蛋氨酸钙(DL-MHA-Ca)相对于DL-蛋氨酸(DLM)的生物学效价。选取792只体重相近且健康的AA肉仔鸡,随机分为11个处理,每个处理6个重复。DLM或DL-MHA-Ca按照等摩尔水平(0... 试验采用肉仔鸡生产性能和胴体品质指标结合回归模型比较了DL-羟基蛋氨酸钙(DL-MHA-Ca)相对于DL-蛋氨酸(DLM)的生物学效价。选取792只体重相近且健康的AA肉仔鸡,随机分为11个处理,每个处理6个重复。DLM或DL-MHA-Ca按照等摩尔水平(0.03%、0.06%、0.10%、0.15%和0.21%)添加到仅缺乏蛋氨酸的基础日粮中。试验期35 d,分为7~21 d和22~42 d 2个阶段。结果表明:7~21 d和7~42 d,添加蛋氨酸源显著改善了肉仔鸡的体增重、饲料/增重、屠体重、胸肉重、胸肉率、腿肉重和全净膛重(P〈0.05);在等摩尔基础上,DL-MHA-Ca相对于DLM的生物学效价,7~21 d分别是71%(增重)和66%(饲料/增重);7~42 d则分别为63%(增重)、58%(饲料/增重)、74%(屠体重)、60%(胸肉重)、49%(胸肉率)、71%(腿肉重)和72%(全净膛重)。 展开更多
关键词 DL-蛋氨酸 DL-羟基蛋氨酸钙 生物学效价 肉仔鸡
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北京鸭蛋氨酸羟基类似物相对蛋氨酸生物学效价的研究 预览
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作者 谢明 侯水生 +2 位作者 黄苇 赵玲 喻俊英 《中国饲料》 北大核心 2008年第4期 4-6,10,共4页
本试验通过研究DL-蛋氨酸(DLM)及DL-蛋氨酸羟基类似物游离酸(DL-HMB-FA)对北京鸭生产性能的影响,进而探讨了蛋氨酸羟基类似物相对蛋氨酸的生物学效价。试验采用单因子完全随机试验设计,设11个日粮处理。其中,1个蛋氨酸缺乏的基础日... 本试验通过研究DL-蛋氨酸(DLM)及DL-蛋氨酸羟基类似物游离酸(DL-HMB-FA)对北京鸭生产性能的影响,进而探讨了蛋氨酸羟基类似物相对蛋氨酸的生物学效价。试验采用单因子完全随机试验设计,设11个日粮处理。其中,1个蛋氨酸缺乏的基础日粮组,5个DLM添加组(0.03%,0.06%,0.10%,0.15%,0.21%),5个添加与DLM水平等摩尔浓度的DL-HMB-FA添加组(0.034%,0.068%,0.113%,0.170%,0.239%)。704只7日龄雄性北京鸭随机分为11个处理,每个处理8个重复,每个重复8只鸭,饲养期为7~42日龄。结果表明,饲料中添加DLM与DL-HMB-FA均可显著提高7~42日龄北京鸭平均日增重(P〈0.05)。同时,采用非线性模型斜率比法,以等摩尔添加水平为基础,以日增重为评价指标,DL-HMB-FA相对DLM生物学效价为67%。 展开更多
关键词 北京鸭 蛋氨酸 蛋氨酸羟基类似物 生物学效价
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