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MicroRNAs as diagnostic and prognostic biomarkers of age-related macular degeneration:advances and limitations 认领
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作者 Bridget Martinez Philip V.Peplow 《中国神经再生研究:英文版》 SCIE CAS 2021年第3期440-447,共8页
A main cause of vision loss in the elderly is age-related macular degeneration(AMD).Among the cellular,biochemical,and molecular changes linked to this disease,inflammation and angiogenesis appear as being crucial in ... A main cause of vision loss in the elderly is age-related macular degeneration(AMD).Among the cellular,biochemical,and molecular changes linked to this disease,inflammation and angiogenesis appear as being crucial in AMD pathogenesis and progression.There are two forms of the disease:dry AMD,accounting for 80–90%of cases,and wet AMD.The disease usually begins as dry AMD associated with retinal pigment epithelium and photoreceptor degeneration,whereas wet AMD is associated with choroidal neovascularization resulting in severe vision impairment.The new vessels are largely malformed,leading to blood and fluid leakage within the disrupted tissue,which provokes inflammation and scar formation and results in retinal damage and detachment.Micro RNAs are dysregulated in AMD and may facilitate the early detection of the disease and monitoring disease progression.Two recent reviews of micro RNAs in AMD had indicated weaknesses or limitations in four earlier investigations.Studies in the last three years have shown considerable progress in overcoming some of these concerns and identifying specific micro RNAs as biomarkers for AMD.Further large-scale studies are warranted using appropriate statistical methods to take into account gender and age disparity in the study populations and confounding factors such as smoking status. 展开更多
关键词 biomarkers blood plasma blood serum macular degeneration MICRORNAS peripheral blood nuclear cells retinal tissues vitreous humour whole blood
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Cardiovascular System Response to Chronic Exposure to Emissions from Gas Turbines Power Plants 认领
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作者 C. D. Ekpruke V. I. Iyawe 《心血管病(英文)》 2020年第4期208-224,共17页
Objectives: Heart Rate and Blood Pressure have been reported to be susceptible to the effects of air pollution. Effect of emissions from gas turbine power station among workers is relatively unexplored. Therefore, thi... Objectives: Heart Rate and Blood Pressure have been reported to be susceptible to the effects of air pollution. Effect of emissions from gas turbine power station among workers is relatively unexplored. Therefore, this study aimed at assessing the effect of emissions from gas turbines on cardiovascular functions and C-reactive protein level in workers of power generating stations. Methodology: 440 individuals made up of 228 workers of gas-fired power plant stations and 212 non-power generating station workers volunteered for this study. A detailed questionnaire was carefully filled by volunteers and anthropometric data measured and recorded. Blood pressure, heart rate and C-reactive protein level (CRP) were measured using standard laboratory techniques in all subjects. Results were presented as Mean ± Standard Error of Mean (SEM) and appropriate analysis was done using Independent student’s t-test and Analysis of Variance (ANOVA). P-value Results: Result showed increased systolic blood pressure (SBP), 142.45 ± 8.87 mmHg of test subjects compared to that of the control (P 0.05) which was 121.32 ± 2.25 mmHg. However, there was no statistically significant difference in the Diastolic Blood Pressure (DBP) of the test and control groups. The Pulse Rate (PR) increased significantly in the test group (89.00 ± 0.02 bpm) when compared to that of the control group (74.34 ± 1.23 bpm). There was also an elevated plasma level of CRP, 6.69 ± 0.03 mg/L in the test group compared to the control (P Conclusion: Exposure to gas emission from natural gas-fired power plants on workers of power generating stations increased SBP, PR and C-reactive Protein level. 展开更多
关键词 EMISSIONS Heart Rate SYSTOLIC BLOOD PRESSURE DIASTOLIC BLOOD PRESSURE Gas Turbines
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The Roles of Key Electrolytes in Balancing Blood Acid-Base and Nutrient in Broiler Chickens Reared under Tropical Conditions 认领
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作者 Ibukun Olukorede Popoola Oluwabukola Rashidat Popoola +2 位作者 Matthew Oluwatobi Ojeniyi Oluwaseyi Olamide Olajide Eustace Ayemere Iyayi 《自然科学期刊(英文)》 2020年第1期4-11,共8页
The loss of electrolyte balance in diets of broiler chickens has resulted in a serious distur-bance of blood acid-base balance, coupled with elevated body temperature. The body losses carbon dioxide (CO2) and bicarbon... The loss of electrolyte balance in diets of broiler chickens has resulted in a serious distur-bance of blood acid-base balance, coupled with elevated body temperature. The body losses carbon dioxide (CO2) and bicarbonate (HCO3), resulting in respiratory alkalosis or acidosis. Under tropical conditions of high environmental temperatures, the balance of electrolytes in feeds must be set much higher as to maintain equal metabolic and digestive efficiency. However, information on the ideal dietary electrolyte balance (DEB) that could effectively correct acid-base imbalance in broiler chickens under severe heat stress condition is scanty. Therefore, the effects of varying electrolyte balance in diets on haematology, blood glucose and serum inorganic elements were assessed in broiler chickens at starter (0 - 21 d) and finisher (22 - 35 d) phases, under temperature-humidity index of 24.97 - 35.19. One day-old Arbor Acre chicks (n = 300) were procured and randomly allotted to diets supplemented with potassium chloride and sodium bicarbonate, to balance monovalent ions (sodium, potassium and chloride) at 210 (T1), 240 (T2), 270 (T3), 300 (T4), 330 (T5) and 360 (T6) mEq/kg DEB, in a completely randomised design. On days 21 and 35, blood (5 mL) samples were collected from birds in each replicate whose weights were closest to the mean class weight for haematology and serum biochemical indices using standard procedures. Data were analysed using descriptive statistics and ANOVA at α = 0.05. Different levels of DEB did not significantly affect (P > 0.05) haematology and blood glucose at starter phase. However, at finisher phase, heterophil: lymphocyte of birds on 270 and 240 mEq/kg DEB were lower (P < 0.05) compared to other dietary treatments. Blood acid-base balance was relatively enhanced in birds on aggregate DEB level of 360 mEq/kg with reduced chloride ion and relatively lower incidence of hemodilution with respect to high haemoglobin levels as this level is advantageous in balancing blood acid to base ratio in broiler c 展开更多
关键词 BLOOD ACID-BASE Monovalent Ions BROILERS BLOOD GLUCOSE Heat Stress Serum MINERALS
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Discussion on the Theory of Pricking Blood in Nei Jing from the Perspective of Time 认领
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作者 Jing-Jie Yang Hai-Yan Ren 《医学史与医学哲学》 2020年第2期47-53,共7页
Objective:The purpose of this paper is to explore the influence of time factors recorded in Nei Jing,such as changes of Yin Yang in four seasons,the wax and wane of the moon and the rhythm of Yin Yang in a day,on pric... Objective:The purpose of this paper is to explore the influence of time factors recorded in Nei Jing,such as changes of Yin Yang in four seasons,the wax and wane of the moon and the rhythm of Yin Yang in a day,on pricking blood therapy.Methods:The contents of pricking blood according to time in Nei Jing is studied by using the method of literature review.Results:Through the research,this paper concludes that(1)pricking blood according to time takes"the correspondence between nature and man"as the dominant thought,"meridian Qi and Blood"as the regulating object,and"time factor"as the specie operation key,which reflects the influence of time on pricking blood therapy;(2)pricking blood therapy has different speci行c operations according to different time,which can be roughly divided into three situations.One is to prick blood according to the change of seasons,pricking shallowly with expelling less blood in spring and summer and pricking deeply with squeezing more blood out in autumn and winter.The second is pricking blood according to the laws of moon's wax and wane.When the moon is born,the amount of bleeding should be less;when the moon is full,the amount of bleeding should be more;when the moon wanes,pricking blood therapy should not be used.Thirdly,take pricking blood therapy according to circadian rhythm.It is more suitable to use pricking blood therapy in the morning than afternoon.Conclusion:As a characteristic therapy of TCM,pricking blood embodies the idea of"the correspondence between nature and man"in TCM.Adopting different pricking blood rules according to the change of time can improve the clinical therapeutic effect.The thought of pricking according to time plays an important guiding role in the application of pricking blood therapy in clinic. 展开更多
关键词 Nei Jing CIRCADIAN RHYTHM Pricking BLOOD Pricking BLOOD according to TIME CORRESPONDENCE between nature and man MERIDIAN Qi and BLOOD
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文章速递从气、血、水论治肝硬变腹水 认领
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作者 翟怀乐 周晓玲 《河南中医》 北大核心 2020年第10期1478-1481,共4页
肝硬变腹水的主要病机在于气(阳)虚、血虚、血瘀、水停。气(阳)虚则推动及生化无力,导致血虚、血瘀、水停;水停日久,可导致气血生成不足;血瘀日久,可导致新血不生及水停加重。临证治疗以气、血、水三者关系为核心,从气(阳)、血、水论治... 肝硬变腹水的主要病机在于气(阳)虚、血虚、血瘀、水停。气(阳)虚则推动及生化无力,导致血虚、血瘀、水停;水停日久,可导致气血生成不足;血瘀日久,可导致新血不生及水停加重。临证治疗以气、血、水三者关系为核心,从气(阳)、血、水论治,每获良效。阳虚水盛夹瘀证多为臌胀病初中期,治以温补脾肾、化气利水、活血化瘀,方选四逆汤合补阳还五汤合五苓散加减;阳虚血虚水盛证多为臌胀病中后期,治以温补脾肾、化气利水、养血柔肝,方选四逆汤合当归芍药散加减。 展开更多
关键词 肝硬变腹水 “气、血、水”理论 阳虚水盛夹瘀证 阳虚血虚水盛证
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Kidney Function in Frequent Users of Non-Steroidal Anti-Inflammatory Drugs (NSAIDs) 认领
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作者 Uduagbamen PK Salako BL +2 位作者 Hamzat MA Kadiri S Arogundade FA 《内科学期刊(英文)》 2020年第1期69-82,共14页
Background: Non-steroidal anti-inflammatory drugs (NSAIDs) are used for managing painful conditions. They are available as cheap, over-the-counter drugs, and commonly abused. NSAIDs inhibit prostaglandins (PGs) action... Background: Non-steroidal anti-inflammatory drugs (NSAIDs) are used for managing painful conditions. They are available as cheap, over-the-counter drugs, and commonly abused. NSAIDs inhibit prostaglandins (PGs) actions on the kidneys and can cause kidney disease and hypertension, especially when used in excess doses, for prolonged period or in stressed states. Methods: The descriptive study was carried at the Orthopaedic and Family Medicine units of the Federal Medical Centre, Abeokuta. Two hundred respondents participated in the study. One hundred frequent users of NSAIDs (with daily use for ≥ 4 weeks) and age and sex-matched controls with no known risk for kidney disease and had consented were consecutively recruited. Data were entered from history, examination and investigations (urinalysis, serum electrolyte, kidney scan and biopsy). Cases with estimated glomerular filtration rate (eGFR) 2) and dip strip proteinuria ≥ 1+ had kidney biopsy. Statistical analysis was with SPSS 21 software. Student t-test and Chi-square tests were used to compare means and proportions respectively. Pearson’s correlation test was used to determine the strength of association between independent risk factors and kidney dysfunction (KD). Results: Two hundred respondents participated in the study. Fifty one (51) females and Forty nine (49) males were recruited as cases and controls respectively. Thirteen (13) females had KD compared to 9 males, (P = 0.02). The mean age of cases with KD (63.04 yrs ± 4.21) was statistically higher than those without KD (P = 0.01). Majority of the cases were in the working population (30 - 59 yrs). Twenty two (22) frequent NSAIDs users had kidney dysfunction (KD) while six (6%) controls had KD. The proportion of subjects that used herbal medicines was higher in cases with KD than in cases without KD as well as in the controls respectively (P = 0.01). The mean kidney length and cortical thickness were significantly lower in cases with KD than in cases without KD, (P = 0.03) and (P = 0.017) respect 展开更多
关键词 NON-STEROIDAL ANTI-INFLAMMATORY Drugs Glomerular FILTRATION Rate Kidney DYSFUNCTION Body Mass Index Blood Pressure
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Trans-Thoracic Echocardiographic Aortic Blood Flow Peak Velocity Variation, Distance Minute, Aortic Velocity Time Integral and Postoperative Outcome in Pediatric Surgical Patients—An Observational Pilot Study Protocol 认领
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作者 Claudine Kumba 《内科学期刊(英文)》 2020年第1期90-95,共6页
Background: A Randomized Controlled Trial (RCT) has been elaborated where goal directed fluid and hemodynamic therapy (GDFHT) will be realized with trans-thoracic echocardiographic aortic blood flow peak velocity vari... Background: A Randomized Controlled Trial (RCT) has been elaborated where goal directed fluid and hemodynamic therapy (GDFHT) will be realized with trans-thoracic echocardiographic aortic blood flow peak velocity variation (ΔVpeak) and distance minute (DM) to guide fluid therapy and hemodynamics in high risk pediatric surgical patients. This RCT will clarify the impact of GDFHT with ΔVpeak and DM on postoperative outcome in terms of morbidity, length of stay in the intensive care unit (LOSICU), length of mechanical ventilation (LMV) and length of hospital stay (LOS) in children. To determine values of ΔVpeak, DM and VTI predictive of these postoperative outcomes, an observational pilot study will be realized. This pilot study is described here. The primary objective of this study is to determine values of ΔVpeak, DM and ITV predictive of postoperative outcome in children in terms of morbidity. The secondary objectives are to determine values of ΔVpeak, DM and ITV predictive of LOSICU, LMV, LOS, intraoperative, postoperative fluid administration and vasoactive-inotropic therapy. Methods: 500 - 1000 children aged less than 18 years will be included prospectively. Statistic analysis will be realized with XLSTAT 2019.4.2 software or plus. Results and Conclusions: This trial protocol will determine values of ΔVpeak, DM and ITV with echocardiography predictive of postoperative outcome in children. 展开更多
关键词 Children AORTIC Blood Flow Peak VELOCITY Variation AORTIC VELOCITY Time INTEGRAL Distance MINUTE Postoperative Outcome Pilot Study Protocol Trans-Thoracic Echocardiography
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Outcome of Maternal Anaemia on Cord Haemoglobin of the Newborn in Port Harcourt, Nigeria 认领
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作者 Justina Omoikhefe Alegbeleye Dagogo Semenitari Abam Ngozi Clare Orazulike 《生物科学与医学(英文)》 2020年第5期142-152,共11页
Background: Anaemia is a common medical disorder in pregnancy especially in developing countries. Iron deficiency anaemia during pregnancy may be associated with adverse pregnancy outcomes Objectives: To?determine the... Background: Anaemia is a common medical disorder in pregnancy especially in developing countries. Iron deficiency anaemia during pregnancy may be associated with adverse pregnancy outcomes Objectives: To?determine the cord blood haemoglobin and the correlation between maternal haemoglobin concentration and perinatal outcome at the University of Port Harcourt Teaching Hospital. Materials and Methods: This was a prospective cross-sectional study of 155 pregnant women who presented at the labour ward of the department of Obstetrics and Gynaecology from October 1, 2018 to December 31, 2018. Blood sample was collected from the women in labour into ethylenediamine tetra-acetic acid (EDTA) bottles, red blood cell haemoglobin was determined. Umbilical cord blood sample was also collected at delivery and red cell haemoglobin was done. Socio-demographic characteristics, risk factors and perinatal outcome were obtained, entered into a spreadsheet and analyzed using SPSS 22.0. Bivariate logistic regression analysis was performed for multivariate analysis and statistical significance was considered at p Results: The mean age of the women was 31.08 ± 4.39 years, the mean maternal haemoglobin concentration was 11.54 ± 1.86 g/dl while the mean umbilical cord haemoglobin concentration was 12.92 ± 1.92 g/dl. Majority (86.45%) of the women were compliant with the prescribed haematinics. Moderate anaemia was observed in 17.42% of the women, followed by mild anaemia (15.48%) and severe anaemia (1.29%) respectively. Maternal complications were observed in 24.5% of women. There was a significant association between maternal haemoglobin concentration and cord blood haemoglobin concentration (p = 0.001). However, the study did not show an association between parity, mode of delivery, birth weight, Apgar scores, gestational age and maternal haemoglobin concentration (p > 0.05). Conclusion: The mean Haemoglobin in our newborns was less than previously reported values. Cord blood haemoglobin decreases significantly with decreasing matern 展开更多
关键词 MATERNAL HAEMOGLOBIN CORD Blood HAEMOGLOBIN Perinatal OUTCOME NIGERIA
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The Role of Blood Station Quality Management System in the Quality Control of Blood Collection 认领
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作者 Li Zhang 《医学科学进展杂志(英文)》 2020年第1期48-52,共5页
With the continuous development of China’s medical industry in recent years,relevant staff has also paid more attention to the quality management of blood stations,and China has gradually improved during the setting ... With the continuous development of China’s medical industry in recent years,relevant staff has also paid more attention to the quality management of blood stations,and China has gradually improved during the setting of laws and regulations for blood station quality management.The policies set by the Chinese government have been fully implemented through the reasonable implementation of relevant staff.With the continuous development of China’s medical industry in recent years,relevant staff have also paid more attention to the quality management of blood stations,and China has gradually improved during the setting of laws and regulations for blood station quality management.The policies set by the Chinese government have been fully implemented through the reasonable implementation of relevant staff.On this basis,the management model of the Chinese blood station has been continuously innovated in the application process,and the blood collection work of the Chinese blood station has been greatly developed.However,when the blood station in China is conducting blood collection,its quality control program is still not fully mature.Therefore,in this context,it is necessary to do a good job in the construction of the corresponding blood station quality management system and make effective adjustments,which will give full play to the role of the management system in the quality control process of blood collection.In this paper,the construction plan of the blood station quality management system is analyzed to explore the role of the blood station quality management system in the process of blood collection quality control,aiming to provide assistance for the quality management of blood collection in China. 展开更多
关键词 BLOOD STATION QUALITY MANAGEMENT system BLOOD COLLECTION QUALITY control ROLE analysis
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Progress of studies on cancer-related anemia in Chinese and Western medicine 认领
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作者 Kai Zhou Jia-Yu Zhang +2 位作者 Jun-Jin Li Yan-Fang Cheng Pei-Xian Zhang 《精准医学研究》 2020年第3期136-142,共7页
Cancer-related anemia is a common complication in patients with a malignant tumor caused by the tumor itself or the tumor's treatment.It can seriously affect the quality of life of patients and even accelerate the... Cancer-related anemia is a common complication in patients with a malignant tumor caused by the tumor itself or the tumor's treatment.It can seriously affect the quality of life of patients and even accelerate the progression of the tumor.Western medicine is often given a blood transfusion,erythropoietin,and iron agent in cancer-related anemia treatment,but there are also some problems such as increasing tumor-related mortality.Traditional Chinese medicine believes that cancer-related anemia's occurrence is related to the“Deficiency of Spleen and Kidney and Deficiency of Qi and Blood”,so clinical treatment is mainly to“Tonify Spleen and Kidney and Qi and Blood”.The combination of traditional Chinese and Western medicine is useful in cancer-related anemia's treatment,which provides a new idea and method for cancer-related anemia's clinical treatment. 展开更多
关键词 Cancer-related anemia Blood transfusion ERYTHROPOIETIN Deficiency of Spleen and Kidney Deficiency of Qi and Blood
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Study on the Biological Basis of Qi, Blood, and Vessel in Immune Thrombocytopenia Patients With Syndrome of Qi Failing to Govern Blood Based on the Theory of Qi and Blood 认领
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作者 王明镜 陈卓 +7 位作者 朱世荣 丁晓庆 谌海燕 全日城 许勇钢 赵攀 姜云耀 胡晓梅 《世界中西医结合杂志(英文)》 2020年第1期18-25,共8页
Objective: To investigate the biological basis of qi, blood and vessel in immune thrombocytopenia(ITP) patients with syndrome of qi failing to govern blood(SQFGB) based on traditional Chinese medicine.Methods: A total... Objective: To investigate the biological basis of qi, blood and vessel in immune thrombocytopenia(ITP) patients with syndrome of qi failing to govern blood(SQFGB) based on traditional Chinese medicine.Methods: A total of 52 ITP patients with SQFCB were enrolled and divided into bleeding group(38 cases) and non-bleeding group(14 cases).Bleeding group was further divided into mild qi deficiency group(25 cases) and moderate/severe qi deficiency group(13 cases) based on Chinese Medicine syndrome score.20 healthy volunteer were recruited as control group.The count of platelet(PLT) was taken as the blood related indicator.The expressions of cytokines including IL-1β, IL-17 A, TNF-α, CD40 L, and TGF-β, detected by Aim Plex Multiple Immunoassays for Flow, were taken as the qi related indicators.The expressions of VEGF-A, detected by Aim Plex Multiple Immunoassays for Flow and NO, NOS, and ET-1 detected by ELISA, were taken as the vessel related indicators.Results: As compared to the control group, the count of PLT, taken as the blood related indicator, was significantly lower in ITP group patients with SQFCB(P<0.05).The expression levels of IL-17 A and TNF-α, taken as the qi related indicators, were significantly higher, while those of CD40 L, IL-1β, and TGF-β, also taken as the qi related indicators, were significantly lower in ITP patients with SQFCB, respectively(P<0.05).The expression levels of NO and ET-1, taken as the vessel related indicators, were significantly higher, while the expression levels of NOS and VEGF-A also taken as the vessel related indicators, were significantly lower in ITP patients with SQFCB, respectively(P<0.05).The count of PLT, taken as the blood related indicator, was significantly lower in moderate/severe group than those in mild group(P<0.05).The expression levels of CD40 L and TGF-β, taken as the qi related indicators, were also significantly lower in moderate/severe group than those in mild group, respectively(P<0.05).Conclusion: The count of PLT might be the biological basis of b 展开更多
关键词 Syndrome of QI failing to govern BLOOD IMMUNE THROMBOCYTOPENIA Theory of QI and BLOOD Chinese MEDICINE
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Improvement and Application of Acute Blood Stasis Rat Model Aligned with the 3Rs (Reduction, Refinement and Replacement) of Humane Animal Experimentation 认领
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作者 HUANG Shuai XU Feng +4 位作者 WANG Yin-ye SHANG Ming-ying WANG Chao-qun WANG Xuan CAI Shao-qing 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2020年第4期292-298,共7页
Objective:To establish a novel cardiocentesis method for withdrawing venous blood from the right atrium,and to improve an acute blood stasis rat model using an ice bath and epinephrine hydrochloride(Epi)while consider... Objective:To establish a novel cardiocentesis method for withdrawing venous blood from the right atrium,and to improve an acute blood stasis rat model using an ice bath and epinephrine hydrochloride(Epi)while considering the 3Rs(reduction,refinement,and replacement)of humane animal experimentation.Methods:An acute blood stasis model was established in male Sprague-Dawley rats by subcutaneous injection(s.c.)Epi(1.2mg/kg)administration at 0h,followed by a 5-min exposure to an ice-bath at 2h and s.c.Epi administration at 4h.Control rats received physiological saline.Rats were fasted overnight and treated with Angelicae Sinensis Lateralis Radix(ASLR)and Pheretima the following day.Venous blood was collected using our novel cardiocentesis method and used to test whole blood viscosity(WBV),prothrombin time(PT),activated partial thromboplastin time(APTT),and fibrinogen(FIB)8ntent.Results:The rats survived the novel cardiocentesis technique;WBV value returned to normal while hematological parameters such as hemoglobin level and red blood cell count were restored to>94%of the corresponding values in normal rats following a 14-day recovery.Epi(1.2 mg/kg,s.c.)combined with a 5-min exposure to the ice bath replicated the acute blood stasis rat model and was associated with the highest WBV value.In rats showing acute blood stasis,ASLR treatment[4g/(kg-d)for 8 days]decreased WBV by 9.98%,11.09%,9.34%,9.00%,7.66%,and 7.03%(P<0.05),while Pheretima treatment[2.6g/(kg?d),for 8 days]decreased WBV by 25.49%,25.94%,16.28%,17.76%,11.07%,and 7.89%(P<0.01)at shear rates of 1,3,10,30,100,and 180 s'1,respectively.Furthermore,Pheretima treatment increased APTT significantly(P<0.01).Conclusions:We presented a stable,reproducible,and improved acute blood stasis rat model,which could be applied to screen drugs for promoting blood circulation and eliminating blood stasis. 展开更多
关键词 acute BLOOD STASIS rat model cardiocentesis whole BLOOD viscosity COAGULATION parameters Angelicae Sinensis Lateralis Radix PHERETIMA Chinese medicine syndrome
MicroRNAs as disease progression biomarkers and therapeutic targets in experimental autoimmune encephalomyelitis model of multiple sclerosis 认领
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作者 Bridget Martinez Philip V.Peplow 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第10期1831-1837,共7页
Multiple sclerosis is an autoimmune neurodegenerative disease of the central nervous system characterized by pronounced inflammatory infiltrates entering the brain,spinal cord and optic nerve leading to demyelination.... Multiple sclerosis is an autoimmune neurodegenerative disease of the central nervous system characterized by pronounced inflammatory infiltrates entering the brain,spinal cord and optic nerve leading to demyelination.Focal demyelination is associated with relapsing-remitting multiple sclerosis,while progressive forms of the disease show axonal degeneration and neuronal loss.The tests currently used in the clinical diagnosis and management of multiple sclerosis have limitations due to specificity and sensitivity.MicroRNAs(miRNAs)are dysregulated in many diseases and disorders including demyelinating and neuroinflammatory diseases.A review of recent studies with the experimental autoimmune encephalomyelitis animal model(mostly female mice 6–12 weeks of age)has confirmed miRNAs as biomarkers of experimental autoimmune encephalomyelitis disease and importantly at the pre-onset(asymptomatic)stage when assessed in blood plasma and urine exosomes,and spinal cord tissue.The expression of certain miRNAs was also dysregulated at the onset and peak of disease in blood plasma and urine exosomes,brain and spinal cord tissue,and at the post-peak(chronic)stage of experimental autoimmune encephalomyelitis disease in spinal cord tissue.Therapies using miRNA mimics or inhibitors were found to delay the induction and alleviate the severity of experimental autoimmune encephalomyelitis disease.Interestingly,experimental autoimmune encephalomyelitis disease severity was reduced by overexpression of miR-146a,miR-23b,miR-497,miR-26a,and miR-20b,or by suppression of miR-182,miR-181c,miR-223,miR-155,and miR-873.Further studies are warranted on determining more fully miRNA profiles in blood plasma and urine exosomes of experimental autoimmune encephalomyelitis animals since they could serve as biomarkers of asymptomatic multiple sclerosis and disease course.Additionally,studies should be performed with male mice of a similar age,and with aged male and female mice. 展开更多
关键词 animal model BLOOD plasma BLOOD serum brain tissue disease biomarkers experimental AUTOIMMUNE ENCEPHALOMYELITIS MICRORNAS multiple SCLEROSIS spinal cord therapeutic targets urine EXOSOMES
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Screening Donated Blood for Transfusion-Transmissible Cytomegalovirus Infection among Libyans 认领
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作者 Farag Bleiblo Abdelhakim Eljaki +5 位作者 Mohamed Bumadian Khaled Elwaheishi Eman Almismary Mabroka Aljlale Rabea Alghazal Mohammed Abraheem 《生物科学与医学(英文)》 2020年第1期5-12,共8页
Human cytomegalovirus (HCMV) is a ubiquitous DNA-containing herpesvirus causes severe and fatal diseases in immunocompromised patients and a prevalent cause of virus-associated birth defects. Blood transfusion donated... Human cytomegalovirus (HCMV) is a ubiquitous DNA-containing herpesvirus causes severe and fatal diseases in immunocompromised patients and a prevalent cause of virus-associated birth defects. Blood transfusion donated for neonates, pregnant women, and immunocom-promised patients should be adequately screened for evidences of CMV infection prior to use in clinical management. The effective national programmes for quality-assured screening of donated blood have not yet been fully established, hence this study was undertaken to assess whether any bloodborne-CMV infections pose a significant threat to the safety of the blood supplies. A total of 200 voluntary blood donor subjects admitted to the Blood Bank of Benghazi/Libya were screened for transfusion-transmissible CMV (TT-CMV) using a highly sensitive CMV total IgG and IgM antibody enzyme immunoassay as well as CMV pp65 anti-genemia assays. We determined that the overall seropositivity for IgG antibodies (80.50%) was higher than that of IgM antibodies (39.00%), but only 2 (1.00%) individuals out of these donors were seropositive for the CMV-antigenic protein pp65. The frequency of CMV infection based on gender was incomparable due to the small population number of blood-donated females. According to age, there was not influence of various age groups on prevalence of anti-CMV IgG antibodies, while a progressive increase in seropositivity of CMV-IgM antibodies with age was detected. The age groups were not significantly associated with CMV prevalence. In contrast, only 2 (1.00%) patients were shown to be positive for all three performed assays indicating a recurrent infection. Our findings prove a risk of primary transfusion-associated transmission of CMV and may provide a policy guidance on ensuring safe blood supplies accessible to all patients who require transfusion. 展开更多
关键词 CYTOMEGALOVIRUS SEROPREVALENCE BLOOD TRANSFUSION Transfusion-Transmissible CYTOMEGALOVIRUS ENZYME Immunoassays
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Gene-modified leucoconcentrate for personalized ex vivo gene therapy in a mini pig model of moderate spinal cord injury 认领
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作者 Rustem R.Islamov Farid V.Bashirov +11 位作者 Mikhail E.Sokolov Andrei A.Izmailov Filip O.Fadeev Vage A.Markosyan Maria A.Davleeva Olga V.Zubkova Maxim M.Smarov Denis Yu.Logunov Boris S.Naroditskyi Ilnur I.Salafutdinov Albert A.Rizvanov Ramil G.Turaev 《中国神经再生研究:英文版》 SCIE CAS 2021年第2期357-361,共5页
We previously demonstrated that gene-modified umbilical cord blood mononuclear cells overexpressing a combination of recombinant neurotrophic factors are a promising therapeutic approach for cell-mediated gene therapy... We previously demonstrated that gene-modified umbilical cord blood mononuclear cells overexpressing a combination of recombinant neurotrophic factors are a promising therapeutic approach for cell-mediated gene therapy for neurodegenerative diseases,neurotrauma,and stroke.In this study,using a mini pig model of spinal cord injury,we proposed for the first time the use of gene-modified leucoconcentrate prepared from peripheral blood in the plastic blood bag for personalized ex vivo gene therapy.Leucoconcentrate obtained from mini pig peripheral blood was transduced with a chimeric adenoviral vector(Ad5/35 F)that carried an enhanced green fluorescent protein(EGFP)reporter gene in the plastic blood bag.The day after blood donation,the mini pigs were subjected to moderate SCI and four hours post-surgery they were intravenously autoinfused with gene-modified leucoconcentrate.A week after gene-modified leucoconcentrate therapy,fluorescent microscopy revealed EGFP-expressing leucocytes in spinal cord at the site of contusion injury.In the spleen the groups of EGFP-positive cells located in the lymphoid follicles were observed.In vitro flow cytometry and fluorescent microscopy studies of the gene-modified leucoconcentrate samples also confirmed the production of EGFP by leucocytes.Thus,the efficacy of leucocytes transduction in the plastic blood bag and their migratory potential suggest their use for temporary production of recombinant biologically active molecules to correct certain pathological conditions.This paper presents a proof-of-concept of simple,safe and effective approach for personalized ex vivo gene therapy based on gene-modified leucoconcentrate autoinfusion.The animal protocols were approved by the Kazan State Medical University Animal Care and Use Committee(approval No.5)on May 27,2014. 展开更多
关键词 chimeric Ad5/35F virus enhanced green fluorescent protein gene-modified leucoconcentrate mini pig peripheral blood personalized ex vivo gene therapy plastic blood bag spinal cord injury
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In-Host Analysis of Malaria Dynamics in Humans 认领
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作者 Kodwo Annan 《应用科学(英文)》 2020年第5期191-209,共19页
Human malaria infection poses a major global health threat worldwide. Yet, no sophisticated mathematical model exists to study the complex dynamics and interactions between the parasites and host immune response at th... Human malaria infection poses a major global health threat worldwide. Yet, no sophisticated mathematical model exists to study the complex dynamics and interactions between the parasites and host immune response at the blood and liver stages. In this paper, an in-host mathematical model of Plasmodium falciparum malaria dynamics and interactions in an infected host cells are studied at the liver stage by incorporating the red blood cells and the immune system. Numerical simulations are applied to investigate the interactions between the host immune response, the parasite dynamics, and the disease dynamics at both the blood and liver stages. Results show that immunity has a significant impact in clearing infected red blood cells. Furthermore, the infected erythrocytes and hence the severity of malaria tend to increase with increasing density of merozoites in the blood. The result revealed that intervention during malaria infection should focus on minimizing merozoite invasion rate on healthy erythrocytes and the density of merozoites in circulation. 展开更多
关键词 MALARIA Red BLOOD CELLS MATHEMATICAL Modeling MEROZOITE IMMUNE CELLS
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Effects of <i>γ</i>-Polyglutamic Acid on Blood Glucose and Caecal Short Chain Fatty Acids in Adult Male Mice 认领
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作者 Motoi Tamura Sachiko Hori +1 位作者 Atsuko Inose Masuko Kobori 《食品与营养科学(英文)》 2020年第1期8-22,共15页
γ-Polyglutamic acid (γ-PGA) is a major component of Natto. We hypothesized that γ-PGA could reduce postprandial glucose rise and plasma glucose levels. Mice were fed a 0.1% γ-PGA—containing diet or control diet f... γ-Polyglutamic acid (γ-PGA) is a major component of Natto. We hypothesized that γ-PGA could reduce postprandial glucose rise and plasma glucose levels. Mice were fed a 0.1% γ-PGA—containing diet or control diet for 91 days. Maltose and starch tolerance tests were performed, and plasma lipids, glucose levels, and caecal short chain fatty acids (SCFAs) were measured. Mice were co-administered γ-PGA and starch to suppress the initial rise in blood glucose levels. Blood glucose levels at 15 min were significantly lower in the PGA group than in the Con group (P 0.05). The plasma glucose level and NEFA level were also significantly lower in the PGA group (P 0.05), and caecal acetic acid/total caecal SCFAs ratio was significantly increased in the PGA group (P 0.05). Significant negative correlations existed between the caecal acetic acid/propionic acid ratio and the weight of visceral fat/BW (r =?-0.57, P = 0.0318). Our results suggest that γ-PGA may effectively prevent metabolic syndrome by lowering blood glucose levels. 展开更多
关键词 γ-Polyglutamic Acid Mice Blood GLUCOSE Short Chain FATTY ACIDS
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Saudi Scientific Diabetes Society Position Statement: Management of Diabetes Mellitus in the Pandemic of COVID-19 认领
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作者 Abdulrahman Alshaikh Saud Alsifri +4 位作者 Amani Alhozali Hala Mosli Tarif Zawawi Seraj Mira Emad R. Issak 《临床医学国际期刊(英文)》 2020年第5期199-206,共8页
About 10% coronavirus (COVID-19) infected patients are with diabetes comorbidity. Also, diabetes promotes severe progression in COVID-19 patients. Diabetes comorbidity is associated with significant mortality in those... About 10% coronavirus (COVID-19) infected patients are with diabetes comorbidity. Also, diabetes promotes severe progression in COVID-19 patients. Diabetes comorbidity is associated with significant mortality in those people with COVID-19. In this position statement, the management of diabetes in cases of COVID-19, has been presented. The impact of diabetes on the morbidity and mortality of COVID-19, as well as both the target glucose level and the method of blood glucose control have been presented in details. 展开更多
关键词 COVID-19 DIABETES MELLITUS Glycemic Target Blood GLUCOSE Control
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Assessment of Lipid Profile and Atherogenic Indexes of Hepatitis B Virus Carriers amongst Volunteer Blood Donors in Nigeria 认领
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作者 Kingsley Kalawari Odiabara Edna Ogechi Nwachuku +1 位作者 Ndokiari Boisa Ebirien-Agana Samuel Bartimaeus 《生物科学与医学(英文)》 2020年第4期81-95,共15页
Introduction: Infectious agents such as hepatitis B virus can constitute major public health problems as ii affects the liver resulting into inflammatory conditions. This study was designed to assess plasma lipid prof... Introduction: Infectious agents such as hepatitis B virus can constitute major public health problems as ii affects the liver resulting into inflammatory conditions. This study was designed to assess plasma lipid profile and atherogenic indexes of hepatitis B virus carriers among voluntary blood donors in North Central, North East and South-South regions of Nigeria. Materials & Methods: A total of 183 blood samples were collected from subjects in North Central consisting of 137 males and 46 females;176 blood samples from the North East consisting of 129 males and 47 females and 173 blood samples from subjects in South-South region consisting of 123 males and 50 females. The mean ages of the subjects were 34.89 ± 9.19 years, 39.81 ± 9.60 years and 39.92 ± 9.27 years for North Central, North East and South-South regions respectively. The specimens were tested for hepatitis B surface antigen using enzyme-linked immunosorbent assay. All specimens were analyzed for total cholesterol, HDL-C, LDL-C and triglycerides with standard biochemical procedures. The atherogenic indexes were computed using established ratios. The data was analyzed statistically and p-value Results: The mean values of total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, and triglycerides did not vary significantly (p > 0.05) between the hepatitis B positive carriers and hepatitis B negative subjects across the geo-political zones studied although in some of the parameters the values were on the high normal range between the male and female subjects. The AIP and CRR-1 and CRR-11 of the blood donors in the zones in both the HBsAg positive and negative subjects were within the reference range. The atherogenic coefficient value of >3.0 was obtained for some HBsAg negative and positive subjects in some of the zones. Conclusion: The findings thus indicate that asymptomatic HBV infection may be associated with low risk of dyslipidaemia in the population. 展开更多
关键词 HEPATITIS Atherogenic Indices LIPOPROTEINS CARDIOVASCULAR Disease Blood DONORS
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一例冬季疑似牛焦虫病的检测 认领
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作者 和茂盛 《现代畜牧科技》 2020年第8期1-3,共3页
为了检测某病牛是否感染了焦虫。试验采用无菌采取患病牛抗凝血制备血涂片,进行瑞氏染色,显微镜镜检,选取疑似存在虫体的牛血提取核酸,使用焦虫特异性引物进行PCR扩增,阳性产物测序结果提交NCBI进行比对。血涂片染色后可观察到虫体,然... 为了检测某病牛是否感染了焦虫。试验采用无菌采取患病牛抗凝血制备血涂片,进行瑞氏染色,显微镜镜检,选取疑似存在虫体的牛血提取核酸,使用焦虫特异性引物进行PCR扩增,阳性产物测序结果提交NCBI进行比对。血涂片染色后可观察到虫体,然后使用PCR检测方法可成功扩增特异性目的条带,测序结果进行BLAST比对该基因与XM_947905.1的同源性为91.63%,结果表明该牛患有焦虫病。说明在冬季牛也可以患焦虫病。 展开更多
关键词 焦虫 血液 血涂片 瑞氏染色 PCR检测 BLAST比对
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