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Current status and future prospects of stem cell therapy in Alzheimer’s disease 认领
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作者 Fu-Qiang Zhang Jin-Lan Jiang +3 位作者 Jing-Tian Zhang Han Niu Xue-Qi Fu Lin-Lin Zeng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期242-250,共9页
Alzheimer’s disease is a common progressive neurodegenerative disorder, pathologically characterized by the presence of β-amyloid plaques and neurofibrillary tangles. Current treatment approaches using drugs only al... Alzheimer’s disease is a common progressive neurodegenerative disorder, pathologically characterized by the presence of β-amyloid plaques and neurofibrillary tangles. Current treatment approaches using drugs only alleviate the symptoms without curing the disease, which is a serious issue and influences the quality of life of the patients and their caregivers. In recent years, stem cell technology has provided new insights into the treatment of neurodegenerative diseases, including Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis. Currently, the main sources of stem cells include neural stem cells, embryonic stem cells, mesenchymal stem cells, and induced pluripotent stem cells. In this review, we discuss the pathophysiology and general treatment of Alzheimer’s disease, and the current state of stem cell transplantation in the treatment of Alzheimer’s disease. We also assess future challenges in the clinical application and drug development of stem cell transplantation as a treatment for Alzheimer’s disease. 展开更多
关键词 Alzheimer's disease β-amyloid drug development embryonic STEM CELLS induced PLURIPOTENT STEM CELLS mesenchymal STEM CELLS nerve REGENERATION NEURAL REGENERATION NEURAL STEM CELLS NEURODEGENERATIVE disorders STEM cell therapy
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Printing photovoltaics by electrospray 认领
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作者 Xinyan Zhao Weiwei Deng 《光电进展(英文)》 2020年第6期18-39,共22页
Solution processible photovoltaics(PV)are poised to play an important role in scalable manufacturing of low-cost solar cells.Electrospray is uniquely suited for fabricating PVs due to its several desirable characteris... Solution processible photovoltaics(PV)are poised to play an important role in scalable manufacturing of low-cost solar cells.Electrospray is uniquely suited for fabricating PVs due to its several desirable characteristics of an ideal manufacturing process such as compatibility with roll-to-roll production processes,tunability and uniformity of droplet size,capability of operating at atmospheric pressure,and negligible material waste and nano structures.This review begins with an introduction of the fundamentals and unique properties of electrospray.We put emphasis on the evaporation time and residence time that jointly affect the deposition outcome.Then we review the efforts of electrospray printing polymer solar cells,perovskite solar cells,and dye sensitized solar cells.Collectively,these results demonstrate the advantages of electrospray for solution processed PV.Electrospray has also exhibited the capability of producing uniform films as well as nanostructured and even multiscale films.So far,the electrospray has been found to improve active layer morphology,and create devices with efficiencies comparable with that of spin-coating.Finally,we discuss challenges and research opportunities that enable electrospray to become a mainstream technique for industrial scale production. 展开更多
关键词 photovoltaics ELECTROSPRAY solution process polymer solar cells perovskite solar cells dye sensitized solar cells
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Conjugated Polymers as Hole Transporting Materials for Solar Cells 认领
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作者 Dan Ti Kun Gao +1 位作者 Zhi-Pan Zhang Liang-Ti Qu 《高分子科学:英文版》 SCIE CAS CSCD 2020年第5期449-458,I0005,共11页
In principle,conjugated polymers can work as electron donors and thus as low-cost p-type organic semiconductors to transport holes in photovoltaic devices.With the booming interests in high-efficiency and low-cost sol... In principle,conjugated polymers can work as electron donors and thus as low-cost p-type organic semiconductors to transport holes in photovoltaic devices.With the booming interests in high-efficiency and low-cost solar cells to tackle global climate change and energy shortage,hole transporting materials(HTMs)based on conjugated polymers have received increasing attention in the past decade.In this perspective,recent advances in HTMs for a range of photovoltaic devices including dye-sensitized solar cells(DSSCs),perovskite solar cells(PSCs),and silicon(Si)/organic heterojunction solar cells(HSCs)are summarized and perspectives on their future development are also presented. 展开更多
关键词 Conjugated polymers Hole transporting materials Dye-sensitized solar cells Perovskite solar cells Silicon/organic heterojunction solar cells
Outgrowth endothelial cells form a functional cerebral barrier and restore its integrity after damage 认领
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作者 Rais Reskiawan Abdulkadir Mansour Alwjwaj +2 位作者 Othman Ahmad Othman Kamini Rakkar Ulvi Bayraktutan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第6期1071-1078,共8页
Breakdown of blood-brain barrier,formed mainly by brain microvascular endothelial cells(BMECs),represents the major cause of mortality during early phases of ischemic strokes.Hence,discovery of novel agents that can e... Breakdown of blood-brain barrier,formed mainly by brain microvascular endothelial cells(BMECs),represents the major cause of mortality during early phases of ischemic strokes.Hence,discovery of novel agents that can effectively replace dead or dying endothelial cells to restore blood-brain barrier integrity is of paramount importance in stroke medicine.Although endothelial progenitor cells(EPCs)represent one such agents,their rarity in peripheral blood severely limits their adequate isolation and therapeutic use for acute ischemic stroke which necessitate their ex vivo expansion and generate early EPCs and outgrowth endothelial cells(OECs)as a result.Functional analyses of these cells,in the present study,demonstrated that only OECs endocytosed DiI-labelled acetylated low-density lipoprotein and formed tubules on matrigel,prominent endothelial cell and angiogenesis markers,respectively.Further analyses by flow cytometry demonstrated that OECs expressed specific markers for sternness(CD34),immaturity(CD133)and endothelial cells(CD31)but not for hematopoietic cells(CD45).Like BMECs,OECs established an equally tight in vitro model of human BBB with astrocytes and pericytes,suggesting their capacity to form tight junctions.Ischemic injury mimicked by concurrent deprivation of oxygen and glucose(4 hours)or deprivation of oxygen and glucose followed by reperfusion(20 hours)affected both barrier integrity and function in a similar fashion as evidenced by decreases in transendothelial electrical resistance and increases in paracellular flux,respectively.Wound scratch assays comparing the vasculoreparative capacity of cells revealed that,compared to BMECs,OECs possessed a greater proliferative and directional migratory capacity.In a triple culture model of BBB established with astrocytes,pericytes and BMEC,exogenous addition of OECs effectively repaired the damage induced on endothelial layer in serum-free conditions.Taken together,these data demonstrate that OECs may effectively home to the site of vascular injury and re 展开更多
关键词 cell-based therapy ENDOTHELIAL progenitor CELLS ENDOTHELIUM ischemic stroke neurodegeneration novel therapeutics OUTGROWTH ENDOTHELIAL CELLS regenerative MEDICINE stem CELLS translational MEDICINE
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Transfer of mitochondria from mesenchymal stem cells derived from induced pluripotent stem cells attenuates hypoxia-ischemia-induced mitochondrial dysfunction in PC12 cells 认领
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作者 Yan Yang Gen Ye +5 位作者 Yue-Lin Zhang Hai-Wei He Bao-Qi Yu Yi-Mei Hong Wei You Xin Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期464-472,共9页
Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury.Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology,the mechanisms are not fully... Mitochondrial dysfunction in neurons has been implicated in hypoxia-ischemia-induced brain injury.Although mesenchymal stem cell therapy has emerged as a novel treatment for this pathology,the mechanisms are not fully understood.To address this issue,we first co-cultured 1.5×10^5 PC12 cells with mesenchymal stem cells that were derived from induced pluripotent stem cells at a ratio of 1:1,and then intervened with cobalt chloride(CoCl2)for 24 hours.Reactive oxygen species in PC12 cells was measured by Mito-sox.Mitochondrial membrane potential(ΔΨm)in PC12 cells was determined by JC-1 staining.Apoptosis of PC12 cells was detected by terminal deoxynucleotidal transferase-mediated dUTP nick end-labeling staining.Mitochondrial morphology in PC12 cells was examined by transmission electron microscopy.Transfer of mitochondria from the mesenchymal stem cells derived from induced pluripotent stem cells to damaged PC12 cells was measured by flow cytometry.Mesenchymal stem cells were induced from pluripotent stem cells by lentivirus infection containing green fluorescent protein in mitochondria.Then they were co-cultured with PC12 cells in Transwell chambers and treated with CoCl2 for 24 hours to detect adenosine triphosphate level in PC12 cells.CoCl2-induced PC12 cell damage was dose-dependent.Co-culture with mesenchymal stem cells significantly reduced apoptosis and restoredΔΨm in the injured PC12 cells under CoCl2 challenge.Co-culture with mesenchymal stem cells ameliorated mitochondrial swelling,the disappearance of cristae,and chromatin margination in the injured PC12 cells.After direct co-culture,mitochondrial transfer from the mesenchymal stem cells stem cells to PC12 cells was detected via formed tunneling nanotubes between these two types of cells.The transfer efficiency was greatly enhanced in the presence of CoCl2.More importantly,inhibition of tunneling nanotubes partially abrogated the beneficial effects of mesenchymal stem cells on CoCl2-induced PC12 cell injury.Mesenchymal stem cells reduced CoCl2-induced 展开更多
关键词 apoptosis brain injury HYPOXIA-ISCHEMIA INDUCED pluripotent STEM CELLS mesenchymal STEM CELLS MITOCHONDRIAL membrane potential MITOCHONDRIAL TRANSFER PC12 CELLS tunneling nanotubes
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Recent progress of thin-film photovoltaics for indoor application 认领
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作者 Nanfu Yan Chaowei Zhao +2 位作者 Shengyong You Yuefeng Zhang Weiwei Li 《中国化学快报:英文版》 SCIE CAS CSCD 2020年第3期643-653,共11页
Indoor photovoltaics have attracted increasing attentions owing to their great potential in supplying energy for low power devices under indoor light in our daily life.The third generation thin-film solar cells,includ... Indoor photovoltaics have attracted increasing attentions owing to their great potential in supplying energy for low power devices under indoor light in our daily life.The third generation thin-film solar cells,including dye-sensitized solar cells,perovskite solar cells and organic solar cells,have made rapid progress from the aspect of materials design to photovoltaic performance.This review provides an overview on the recent advances in the development of indoor photovoltaic technologies based on the third generation solar cells.The design principles of advanced thin-film indoor photovoltaics were also summarized according to the characteristics of indoor light and the advantages of the third generation solar cells.Finally,after summarizing the current research progress,the perspective on this topic is provided. 展开更多
关键词 INDOOR photovoltaics DYE-SENSITIZED SOLAR CELLS Perovskites SOLAR CELLS ORGANIC SOLAR CELLS Power CONVERSION efficiency
GFAP expression in the optic nerve and increased H2S generation in the integration centers of the rainbow trout (Oncorhynchus mykiss) brain after unilateral eye injury 认领
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作者 Evgeniya V.Pushchina Anatoly A.Varaksin +1 位作者 Dmitry K.Obukhov Igor M.Prudnikov 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第10期1867-1886,共20页
Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular disorders.However,there are few reports on the effects of H2S in the central nervous system during stress or injury.Previous studies on ... Hydrogen sulfide(H2S)is considered as a protective factor against cardiovascular disorders.However,there are few reports on the effects of H2S in the central nervous system during stress or injury.Previous studies on goldfish have shown that astrocytic response occurs in the damaged and contralateral optic nerves.Glial fibrillary acidic protein(GFAP)concentration in the optic nerves of rainbow trout has not been measured previously.This study further characterized the astrocytic response in the optic nerve and the brain of a rainbow trout(Oncorhynchus mykiss)after unilateral eye injury and estimated the amount of H2S-producing enzyme cystathionineβ-synthase(CBS)in the brain of the rainbow trout.Within 1 week after unilateral eye injury,a protein band corresponding to a molecular weight of 50 kDa was identified in the ipsi-and contralateral optic nerves of the rainbow trout.The concentration of GFAP in the injured optic nerve increased compared to the protein concentration on the contralateral side.The results of a quantitative analysis of GFAP+cell distribution in the contralateral optic nerve showed the largest number of GFAP+cells and fibers in the optic nerve head.In the damaged optic nerve,patterns of GFAP+cell migration and large GFAP+bipolar activated astrocytes were detected at 1 week after unilateral eye injury.The study of H2S-producing system after unilateral eye injury in the rainbow trout was conducted using enzyme-linked immunosorbent assay,western blot analysis,and immunohistochemistry of polyclonal antibodies again st CBS in the integrative centers of the brain:telencephalon,optic tectum,and cerebellum.Enzyme-linked immunosorbent assay results showed a 1.7-fold increase in CBS expression in the rainbow trout brain at 1 week after unilateral eye injury compared with that in intact animals.In the ventricular and subventricular regions of the rainbow trout telencephalon,CBS+radial glia and neuroepithelial cells were identified.After unilateral eye injury,the number of CBS+neuroepithelial cells in the 展开更多
关键词 astrocyte-like CELLS GLIAL fibrillary acidic protein hydrogen sulfide NEUROEPITHELIAL CELLS neuroprotection radial GLIAL CELLS rainbow trout(Oncorhynchus mykiss) reactive oxygen species UNILATERAL eye injury
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Detraining after tumor-bearing accelerates tumor growth while continuous training decreases tumor growth in mice 认领
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作者 Zhanyang Fei Dengke Li +4 位作者 Kaiming Li Ming Zhou Yong Li Yiqun Li Zhenxiao Sun 《中医科学杂志(英文)》 2020年第1期75-81,共7页
Objective:Previous studies have shown that exercise suppresses tumor growth.However,the effects of exercise with different intensities and exercise detraining after tumor-bearing on tumor progression remain unclear.Th... Objective:Previous studies have shown that exercise suppresses tumor growth.However,the effects of exercise with different intensities and exercise detraining after tumor-bearing on tumor progression remain unclear.The purpose of this study was to investigate the effects of continuous and disrupted free and exhausted swimming training after tumor-bearing on tumor progression in melanoma B16-F10-bearing C57BL/6 mice.Methods:C57BL/6 mice were subjected to free or exhausted swimming exercise training for 4 weeks prior to the injection of melanoma B16-F10 cells.Subsequently,the B16-F10-bearing mice were maintained with training consisting of free or exhausted swimming or without exercise for 2 weeks during the tumor challenge.Results:The tumor weight was increased by 42%and 109%in mice with 4-week exhausted swimming prior to B16-F10 tumor cells inoculation followed by 2-week training cessation compared with the tumor-bearing control(P<.05)and continuous training groups(P<.01).Tumor weights in groups with exercise detraining after tumor cell inoculation tended to be increased,while the proliferation of splenic T lymphocytes tended to be decreased compared with the group that maintained exercise intensity.After 6-weeks continuous free or exhausted swimming training,the tumor weight of mice was decreased and the proliferation of splenic T lymphocytes was increased compared with the tumor-bearing control group.The frequency of natural killer cells in tumors was increased in all exercise training groups of mice.Conclusions:These results suggest that maintaining exercise intensity after tumor-bearing slows tumor growth in mice,possibly because of the enhanced proliferative activity of splenic lymphocytes rather than natural killer cell infiltration.However,detraining after tumor-bearing might accelerate tumor progression because of the reduced proliferation of splenic T lymphocytes. 展开更多
关键词 Free swimming exercise training Exhausted swimming exercise training Tumor-bearing mice B16-F10 tumor cells Spleen T lymphocytes Natural killer cells
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免疫细胞衰老表现及免疫功能变化的研究进展 认领
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作者 黄丽映 刘韬 《中华细胞与干细胞杂志:电子版》 2020年第2期119-124,共6页
机体衰老的本质是细胞衰老不断累积的过程。免疫系统的衰老既是机体衰老的必然结果,也是导致机体衰老的重要原因。免疫系统作为衰老变化的主要系统之一受到越来越多的学者重视。本文将从适应性免疫系统的T、B细胞及固有免疫系统的自然杀... 机体衰老的本质是细胞衰老不断累积的过程。免疫系统的衰老既是机体衰老的必然结果,也是导致机体衰老的重要原因。免疫系统作为衰老变化的主要系统之一受到越来越多的学者重视。本文将从适应性免疫系统的T、B细胞及固有免疫系统的自然杀伤(NK)细胞、巨噬细胞、中性粒细胞、树突状细胞(DC)和骨髓源性抑制细胞等免疫细胞的亚群、衰老指标和功能等方面在衰老过程中的改变进行总结,进一步明确免疫系统衰老在机体衰老过程中扮演的重要角色。 展开更多
关键词 免疫衰老 T细胞 NK细胞 炎症
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Photo-driven water splitting photoelectrochemical cells by tandem organic dye sensitized solar cells with I-/I3- as redox mediator 认领
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作者 Shicong Zhang Haonan Ye +2 位作者 Haoran Ding Fengtao Yu Jianli Hua 《中国科学:化学英文版》 SCIE EI CAS CSCD 2020年第2期228-236,共9页
Dye-sensitized photoelectrochemical tandem cells have shown the promise for light driven hydrogen production from water owing to the low cost,wide absorption spectra in the visible region and ease to process of their ... Dye-sensitized photoelectrochemical tandem cells have shown the promise for light driven hydrogen production from water owing to the low cost,wide absorption spectra in the visible region and ease to process of their constitutive photoelectrode materials.However,most photo-driven water splitting photoelectrochemical cells driven by organic dye sensitized solar cells exhibit unsatisfactory hydrogen evolution rate,primarily attributed to their poor light capturing ability and low photocurrent performance.Here we present the construction of a tandem system consisting of an organic blue-colored S5 sensitizer-based dyesensitized photoelectrochemical cell(DSPEC) wired in series with three spectral-complemental dyes BTA-2,APP-3 and APP-1 sensitizers-based dye-sensitized solar cell(DSC),respectively.The two spectral-complemental chromophores were used in DSC and DSPEC to ensure that the full solar spectrum could be absorbed as much as possible.The results showed that the photocurrent of tandem device was closely related to the open-circuit voltage(Voc) of sensitized DSC,in which the tandem configuration consisting of S5 based DSPEC and BTA-2 based DSC gave the best photocurrent.On this basis,tandem device with the only light energy and no external applied electrical bias was further constructed of BTA-2 based 2-junction DSC and S5 based DSPEC and obtained a photocurrent of 500 μA cm-2 for hydrogen generation.Furthermore,I-/I3-was used as a redox couple between dye regeneration and O2 production on the surface of Pt-IrO2/WO3.The strategy opens up the application of pure organic dyes in DSC/DSPEC tandem device. 展开更多
关键词 DYE-SENSITIZED PHOTOELECTROCHEMICAL CELLS water SPLITTING TANDEM device DYE-SENSITIZED solar CELLS
A study on melanoma treatment using dendritic cells loaded with antigens purified from melanoma cell lines 认领
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作者 Yanwei Gao Xia Chen +2 位作者 Weishi Gao Xiangji Lu Lin Peng 《肿瘤学与转化医学:英文版》 2020年第1期21-25,共5页
Objective The aim of this study was to purify effective tumor peptide complexes from human melanoma cell lines to enhance the treatment effects on melanoma.Methods We purified heat shock protein 70(HSP70)-peptide comp... Objective The aim of this study was to purify effective tumor peptide complexes from human melanoma cell lines to enhance the treatment effects on melanoma.Methods We purified heat shock protein 70(HSP70)-peptide complexes(PCs)from human melanoma cell lines A375,A875,M21,M14,WM-35,and SK-HEL-1.We named the purified product as M-HSP70-PCs and determined its immunological activities.Autologous HSP70-PCs purified from primary tumor cells of melanoma patients(9 cases)were used as controls.These two tumor antigenic complexes were loaded into dendritic cells(DCs)and used to stimulate an antitumor response against tumor cells in the corresponding patients.Results Mature DCs pulsed with M-HSP70-PCs stimulated autologous T cells to secrete the same levels of type I cytokines as the autologous HSP70-PCs.Moreover,DCs pulsed with M-HSP70-PCs endued CIK cells with an equal ability as autologous HSP70-PCs to kill melanoma cells in the patients.Conclusion M-HSP70-PCs may be used as an efficient and generalized tumor antigen in the treatment of DC-based malignant melanoma. 展开更多
关键词 heat shock protein 70 peptide complexes DENDRITIC CELLS CIK CELLS MELANOMA cellular IMMUNOTHERAPY
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120例头颈部鼻型结外NK/T细胞淋巴瘤误诊分析 认领
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作者 刘鹏利 程泽星 +2 位作者 陆美萍 张立庆 陈海兵 《临床耳鼻咽喉头颈外科杂志》 CAS 北大核心 2020年第1期73-78,共6页
目的:分析首发头颈部的鼻型结外NK/T细胞淋巴瘤(ENKTL)的特征及误诊原因,以提高认识,减少误诊。方法:回顾分析2010-05-2018-04期间病理确诊为ENKTL,首发部位在头颈部的120例患者。按照确诊之前是否有误诊、误治分为误诊组和非误诊组,比... 目的:分析首发头颈部的鼻型结外NK/T细胞淋巴瘤(ENKTL)的特征及误诊原因,以提高认识,减少误诊。方法:回顾分析2010-05-2018-04期间病理确诊为ENKTL,首发部位在头颈部的120例患者。按照确诊之前是否有误诊、误治分为误诊组和非误诊组,比较分析2组患者在确诊前、后临床特征的差异,分析2组患者在治疗后的总生存率(OS)有无差异。结果:该研究总体误诊率为71.7%,主要误诊为鼻窦炎、鼻炎、鼻息肉、上呼吸道感染等疾病。治疗后1年OS为77.8%,3年OS为65.9%,5年OS为49.3%。误诊组和非误诊组确诊前在性别、年龄、首发症状、首发部位、B症状、浅表淋巴结肿大、体重下降方面比较,均差异无统计学意义(P>0.05);在相对特异性体征、首次就诊医院级别、首次就诊至确诊时间方面差异有统计学意义(P<0.05),首次就诊医院级别中三级医院所占比例误诊组明显低于非误诊组;首次就诊至确诊时间误诊组明显长于非误诊组。确诊后在国际预后指数(IPI)评分、Ki-67表达、治疗方案、接受化疗患者的化疗方案、接受放疗患者的放疗剂量方面比较,2组均差异无统计学意义(P>0.05);在Ann Arbor临床分期方面,2组差异有统计学意义(P<0.05),误诊组临床分期要晚于非误诊组。治疗后,误诊组1年OS为73.6%,3年OS为59.3%,5年OS为43.2%;非误诊组1年OS为88.2%,3年OS为82.4%,5年OS为64.2%;2组OS差异有统计学意义(P<0.05),误诊组OS低于非误诊组,总生存时间低于非误诊组。分别比较Ann Arbor临床分Ⅰ~Ⅱ期和Ⅲ~Ⅳ期的OS 2组之间差异无统计学意义(P>0.05)。结论:首发头颈部的ENKTL因临床、影像学特征非特异性及病理特征复杂性易导致误诊,而误诊导致首次就诊至确诊时间延长,从而引起疾病分期进展,最终可能导致OS下降;避免误诊、早诊断非常重要。 展开更多
关键词 淋巴瘤 结外NK-T细胞 T细胞 自然杀伤细胞 误诊
雷公藤多苷对BALB/c裸小鼠诱发性狼疮的干预机制研究 认领
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作者 陈绪华 孙鑫波 《现代中西医结合杂志》 CAS 2020年第12期1275-1279,共5页
目的探讨雷公藤多苷对BALB/c裸小鼠诱发性狼疮的干预机制。方法将40只BALB/c雌性裸小鼠随机均分为空白组、模型组、激素组和雷公藤多苷组,每组10只。除空白组外,其余组均单次腹腔注射Pristane(0.5 mL/鼠)建立BALB/c裸小鼠诱发性狼疮模... 目的探讨雷公藤多苷对BALB/c裸小鼠诱发性狼疮的干预机制。方法将40只BALB/c雌性裸小鼠随机均分为空白组、模型组、激素组和雷公藤多苷组,每组10只。除空白组外,其余组均单次腹腔注射Pristane(0.5 mL/鼠)建立BALB/c裸小鼠诱发性狼疮模型。成模后,空白组和模型组均给予生理盐水灌胃,激素组给予1%醋酸泼尼松灌胃,雷公藤多苷组给予1%雷公藤多苷灌胃,均1mL/只,1次/d,连续4周。采用Western blot法检测各组小鼠外周血单核细胞糖皮质激素受体(GR)蛋白表达水平,采用ELISA法检测外周血狼疮特异性自身抗体(Sm)蛋白表达水平及免疫球蛋白G(IgG)水平,采用直接免疫荧光方法检测脾脏T细胞亚群分类和B细胞计数,采用SP法和RT-PCR法分别检测腹腔内脂肪肉芽肿结节组织匀浆液中血管内皮生长因子(VEGF)、血管内皮生长因子受体1(VEGFR1)蛋白和mRNA表达水平。结果模型组GR蛋白表达水平明显低于空白组(P<0.05),Sm蛋白表达水平和IgG水平均明显高于空白组(P均<0.05);激素组和雷公藤多苷组GR蛋白表达水平均明显高于模型组(P均<0.05),Sm蛋白表达水平和IgG水平均明显低于模型组(P均<0.05)。模型组裸小鼠脾脏组织中CD4^+、CD8^+百分率和B细胞总数均明显高于空白组(P均<0.05),激素组和雷公藤多苷组CD4^+、CD8^+百分率和B细胞总数均明显低于模型组(P均<0.05)。模型组和激素组VEGF、VEGFR1蛋白及mRNA表达水平均明显高于空白组(P均<0.05),雷公藤多苷组VEGF、VEGFR1蛋白及mRNA表达水平均明显低于模型组和激素组(P均<0.05)。结论雷公藤多苷通过提高GR蛋白表达水平,抑制T、B细胞功能调控免疫反应及减少血管新生而发挥对红斑狼疮的治疗作用。 展开更多
关键词 红斑狼疮 雷公藤多苷 T细胞 B细胞 糖皮质激素受体 血管内皮生长因子
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Gastrointestinal cancer stem cells as targets for innovative immunotherapy 认领
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作者 Mihaela Chivu-Economescu Laura G Necula +5 位作者 Lilia Matei Denisa Laura Dragu Ana I Neagu Irina Alexiu Coralia Bleotu Carmen Cristina Diaconu 《世界胃肠病学杂志:英文版》 SCIE CAS 2020年第14期1580-1593,共14页
The role of cancer stem cells in gastrointestinal cancer-associated death has been widely recognized.Gastrointestinal cancer stem cells(GCSCs)are considered to be responsible for tumor initiation,growth,resistance to ... The role of cancer stem cells in gastrointestinal cancer-associated death has been widely recognized.Gastrointestinal cancer stem cells(GCSCs)are considered to be responsible for tumor initiation,growth,resistance to cytotoxic therapies,recurrence and metastasis due to their unique properties.These properties make the current therapeutic trials against GCSCs ineffective.Moreover,recent studies have shown that targeting stem cell surface markers or stemness associated pathways might have an additional off-target effect on the immune system.Recent advances in oncology and precision medicine have opened alternative therapeutic strategies in the form of cancer immunotherapy.This approach differs from classical anti-cancer therapy through its mechanism of action involving the activation and use of a functional immune system against tumor cells,instead of aiming physically destruction of cancer cells through radio-or chemotherapy.New immunological approaches for GCSCs targeting involve the use of different immune cells and various immune mechanisms like targeting specific surface antigens,using innate immune cells like the natural killer and T cells,T-cell chimeric antigen receptor technology,dendritic cell vaccine,or immune checkpoint inhibitors.In this respect,better understandings of immune regulatory mechanisms that govern anti-tumor response bring new hope in obtaining long-term remission for cancer therapy. 展开更多
关键词 IMMUNOTHERAPY GASTROINTESTINAL CANCER CANCER stem CELLS CAR-T DENDRITIC CELLS vaccines Immune CHECKPOINTS inhibitors
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Prostaglandin E1 attenuates high glucose-induced apoptosis in proximal renal tubular cells by inhibiting the JNK/Bim pathway 认领
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作者 Yu-han Zhang Ya-qin Zhang +4 位作者 Cong-cong Guo Li-kang Wang Yu-jiao Cui Jian-jun Dong Lin Liao 《中国药理学报:英文版》 SCIE CAS CSCD 2020年第4期561-571,共11页
Proximal renal tubular damage is a critical process underlying diabetic kidney disease(DKD).Our previous study shows that prostaglandin E1(PGE1)reduces the apoptosis of renal tubular cells in DKD rats.But its underlyi... Proximal renal tubular damage is a critical process underlying diabetic kidney disease(DKD).Our previous study shows that prostaglandin E1(PGE1)reduces the apoptosis of renal tubular cells in DKD rats.But its underlying mechanisms remain unclear.In this study we investigated the protective effects of PGE1 in DKD rats and high glucose(HG,30 mM)-treated HK-2 proximal tubular cells.Four weeks after uninephrectomized streptozotocin-induced diabetic rats were established,the DKD rats were administered PGE1(10μg·kg^?1·d^?1,iv.)for 10 consecutive days.We showed that PGE1 administration did not change blood glucose levels,but alleviated diabetic kidney injury in the DKD rats,evidenced by markedly reduced proteinuria and renal tubular apoptosis.In the in vitro experiments,PGE1(0.1–100μM)signi?cantly enhanced HG-reduced HK-2 cell viability.In HG-treated HK-2 cells,PGE1(10μM)signi?cantly suppressed the c-Jun N-terminal kinase(JNK)and the mitochondrial apoptosis-related protein expressions such as Bim,Bax,caspase-3 and cleaved caspase-3;similar changes were also observed in the kidney of PGE1-treated DKD rats.By using two pharmacological tools-JNK activator anisomycin(AM)and JNK inhibitor SP600125,we revealed that PGE1 blocked HG-triggered activation of JNK/Bim pathway in HK-2 cells;JNK was an upstream regulator of Bim.In conclusion,our results demonstrate that the nephroprotective effects of PGE1 against apoptosis of proximal renal tubule in DKD rats via suppressing JNK-related Bim signaling. 展开更多
关键词 Diabetic kidney disease PROXIMAL renal TUBULAR CELLS prostaglandin E1 apoptosis JNK/Bim HK-2 PROXIMAL TUBULAR CELLS ANISOMYCIN SP600125
Skeletal muscle-derived cells repair peripheral nerve defects in mice 认领
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作者 Zi-Xiang Chen Hai-Bin Lu +3 位作者 Xiao-Lei Jin Wei-Feng Feng Xiao-Nan Yang Zuo-Liang Qi 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第1期152-161,共10页
Skeletal muscle-derived cells have strong secretory function,while skeletal muscle-derived stem cells,which are included in muscle-derived cells,can differentiate into Schwann cell-like cells and other cell types.Howe... Skeletal muscle-derived cells have strong secretory function,while skeletal muscle-derived stem cells,which are included in muscle-derived cells,can differentiate into Schwann cell-like cells and other cell types.However,the effect of muscle-derived cells on peripheral nerve defects has not been reported.In this study,5-mm-long nerve defects were created in the right sciatic nerves of mice to construct a peripheral nerve defect model.Adult female C57BL/6 mice were randomly divided into four groups.For the muscle-derived cell group,muscle-derived cells were injected into the catheter after the cut nerve ends were bridged with a polyurethane catheter.For external oblique muscle-fabricated nerve conduit and polyurethane groups,an external oblique muscle-fabricated nerve conduit or polyurethane catheter was used to bridge the cut nerve ends,respectively.For the sham group,the sciatic nerves on the right side were separated but not excised.At 8 and 12 weeks post-surgery,distributions of axons and myelin sheaths were observed,and the nerve diameter was calculated using immunofluorescence staining.The number,diameter,and thickness of myelinated nerve fibers were detected by toluidine blue staining and transmission electron microscopy.Muscle fiber area ratios were calculated by Masson’s trichrome staining of gastrocnemius muscle sections.Sciatic functional index was recorded using walking footprint analysis at 4,8,and 12 weeks after operation.The results showed that,at 8 and 12 weeks after surgery,myelin sheaths and axons of regenerating nerves were evenly distributed in the muscle-derived cell group.The number,diameter,and myelin sheath thickness of myelinated nerve fibers,as well as gastrocnemius muscle wet weight and muscle area ratio,were significantly higher in the muscle-derived cell group compared with the polyurethane group.At 4,8,and 12 weeks post-surgery,sciatic functional index was notably increased in the muscle-derived cell group compared with the polyurethane group.These criteria of the muscle-derived cel 展开更多
关键词 MUSCLE NERVE conduit myokine NERVE REGENERATION NERVE REPAIR peripheral NERVE REGENERATION polyurethane catheter seed CELLS SKELETAL MUSCLE SKELETAL muscle-derived CELLS tissue-engineered NERVE
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Stroke gets in your eyes:stroke-induced retinal ischemia and the potential of stem cell therapy 认领
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作者 Chase Kingsbury Matt Heyck +2 位作者 Brooke Bonsack Jea-Young Lee Cesar V.Borlongan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第6期1014-1018,共5页
Stroke persists as a global health and economic crisis,yet only two interventions to reduce stroke-induced brain injury exist.In the clinic,many patients who experience an ischemic stroke often further suffer from ret... Stroke persists as a global health and economic crisis,yet only two interventions to reduce stroke-induced brain injury exist.In the clinic,many patients who experience an ischemic stroke often further suffer from retinal ischemia,which can inhibit their ability to make a functional recovery and may diminish their overall quality of life.Despite this,no treatments for retinal ischemia have been developed.In both cases,ischemia-induced mitochondrial dysfunction initiates a cell loss cascade and inhibits endogenous brain repair.Stem cells have the ability to transfer healthy and functional mitochondria not only ischemic neurons,but also to similarly endangered retinal cells,replacing their defective mitochondria and thereby reducing cell death.In this review,we encapsulate and assess the relationship between cerebral and retinal ischemia,recent preclinical advancements made using in vitro and in vivo retinal ischemia models,the role of mitochondrial dysfunction in retinal ischemia pathology,and the therapeutic potential of stem cell-mediated mitochondrial transfer.Furthermore,we discuss the pitfalls in classic rodent functional assessments and the potential advantages of laser Doppler as a metric of stroke progression.The studies evaluated in this review highlight stem cell-derived mitochondrial transfer as a novel therapeutic approach to both retinal ischemia and stroke.Furthermore,we posit the immense correlation between cerebral and retinal ischemia as an underserved area of study,warranting exploration with the aim of these treating injuries together. 展开更多
关键词 laser Doppler MCAO mesenchymal stem CELLS MITOCHONDRIAL network MITOCHONDRIAL transfer ophthalmic artery optic nerve oxygen-glucose DEPRIVATION regenerative medicine retinal GANGLION CELLS visual impairment
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Tissue-resident memory-like ILCs: innate counterparts of TRM cells 认领
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作者 Xianwei Wang Zhigang Tian Hui Peng 《蛋白质与细胞:英文版》 SCIE CAS CSCD 2020年第2期85-96,共12页
Innate lymphoid cells(ILCs)are defined as lymphocytes that lack RAG recombinase and do not express diverse antigen receptors;however,recent studies have revealed the adaptive features of ILCs.Mouse cytomegalovirus(MCM... Innate lymphoid cells(ILCs)are defined as lymphocytes that lack RAG recombinase and do not express diverse antigen receptors;however,recent studies have revealed the adaptive features of ILCs.Mouse cytomegalovirus(MCMV)-and cytokine-induced memory natural killer(NK)cells circulate in the blood and are referred to as conventional memory NK cells.In contrast,virusand hapten-induced memory NK cells,hapten-induced memory ILC1s,and cytokine-induced memory-like ILC2s exhibit long-term residency in the liver or lung,and are referred to as tissue-resident memory ILCs.Considering their similar migration patterns and memory potential,tissue-resident memory ILCs could be regarded as innate counterparts of resident memory T(T r m)cells.Both tissue-resident memory ILCs and TRM cells share common characteristics in terms of dynamics,phenotype,and molecular regulation.The emergence of ILC memory expands the basic biology of ILCs and prompts us to re-examine their functions in disease progression.This review discusses the evidence supporting tissue-resident memory NK cells and other memory ILC subsets,compares them with TRM cells,and highlights key unsolved questions in this emerging field. 展开更多
关键词 tissue-residency INNATE lymphoid CELLS immunological MEMORY TRM CELLS
Endothelial progenitor cells,potential biomarkers for diagnosis and prognosis of ischemic stroke:protocol for an observational case-control study 认领
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作者 Kamini Rakkar Othman Othman +2 位作者 Nikola Sprigg Philip Bath Ulvi Bayraktutan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第7期1300-1307,共8页
Ischemic stroke is a devastating,life altering event which can severely reduce patient quality of life.Despite years of research there have been minimal therapeutic advances.Endothelial progenitor cells(EPCs),stem cel... Ischemic stroke is a devastating,life altering event which can severely reduce patient quality of life.Despite years of research there have been minimal therapeutic advances.Endothelial progenitor cells(EPCs),stem cells involved in both vasculogenesis and angiogenesis,may be a potential therapeutic target.After a stroke,EPCs migrate to the site of ischemic injury to repair cerebrovascular damage,and their numbers and functional capacity may determine patients'outcome.This study aims to determine whether the number of circulating EPCs and their functional aspects may be used as biomarkers to identify the type(cortical or lacunar)and/or severity of ischemic stroke.The study will also investigate if there are any differences in these characteristics between healthy volunteers over and under 65 years of age.100 stroke patients(50 lacunar and 50 cortical strokes)will be recruited in this prospective,observational case-controlled study.Blood samples will be taken from stroke patients at baseline(within 48 hours of stroke)and days 7,30 and90.EPCs will be counted with flow cytometry.The plasma levels of pro-and anti-angiogenic factors and inflammatory cytokines will also be determined.Outgrowth endothelial cells will be cultured to be used in tube formation,migration and proliferation functional assays.Primary outcome is disability or dependence on day 90 after stroke,assessed by the modified Rankin Scale.Secondary outcomes are changes in circulating EPC numbers and/or functional capacity between patient and healthy volunteers,between patient subgroups and between elderly and young healthy volunteers.Recruitment started in February 2017,167 participants have been recruited.Recruitment will end in November 2019.West Midlands-Coventry&Warwickshire Research Ethics Committee approved this study(REC number:16/WM/0304)on September8,2016.Protocol version:2.0.The Bayraktutan Dunhill Medical Trust EPC Study was registered in ClinicalTrials.gov(NCT02980354)on November 15,2016.This study will determine whether the number of EPCs ca 展开更多
关键词 ageing biomarkers cortical STROKE endothelial progenitor CELLS ischemic STROKE LACUNAR STROKE OBSERVATIONAL study stem CELLS
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Ganoderic acid A protects neural cells against NO stress injury in vitro via stimulating β adrenergic receptors 认领
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作者 Zi-ru Yu Wei-hua Jia +5 位作者 Chao Liu Hong-qing Wang Hai-guang Yang Guo-rong He Ruo-yun Chen Guan-hua Du 《中国药理学报:英文版》 SCIE CAS CSCD 2020年第4期516-522,共7页
Excessive nitric oxide(NO)causes extensive damage to the nervous system,and the adrenergic system is disordered in many neuropsychiatric diseases.However,the role of the adrenergic system in protection of the nervous ... Excessive nitric oxide(NO)causes extensive damage to the nervous system,and the adrenergic system is disordered in many neuropsychiatric diseases.However,the role of the adrenergic system in protection of the nervous system against sodium nitroprusside(SNP)injury remains unclear.In this study,we investigated the effect of ganoderic acid A(GA A)against SNP injury in neural cells and the role of adrenergic receptors in GA A neuroprotection.We found that SNP(0.125?2mM)dose-dependently decreased the viability of both SH-SY5Y and PC12 cells and markedly increased NO contents.Pretreatment with GA A(10μM)signi?cantly attenuated SNP-induced cytotoxicity and NO increase in SH-SY5Y cells,but not in PC12 cells.Furthermore,pretreatment with GA A caused signi?cantly higher adrenaline content in SH-SY5Y cells than in PC12 cells.In order to elucidate the mechanism of GA A-protecting SH-SY5Y cells,we added adrenaline,phentolamine,metoprolol,or ICI 1185511 h before GA A was added to the culture medium.We found that addition of adrenaline(10μM)signi?cantly improved GA A protection in PC12 cells.The addition ofβ1-adrenergic receptor antagonist metoprolol(10μM)orβ2-adrenergic receptor antagonist ICI 118551(0.1μM)blocked the protective effect of GA A,whereas the addition ofα-adrenergic receptor antagonist phentolamine(0.1μM)did not affect GA A protection in SH-SY5Y cells.These results suggest thatβ-adrenergic receptors play an important role in the protection of GA A in SH-SY5Y cells against SNP injuries,and excessive adrenaline system activation caused great damage to the nervous system. 展开更多
关键词 β-adrenergic receptors sodium nitroprusside(SNP) SH-SY5Y CELLS PC12 CELLS ganoderic acid A ADRENALINE norepinephrine PHENTOLAMINE METOPROLOL ICI 118551
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