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Roscovitine对TNF-α诱导的大鼠血管平滑肌细胞增殖及细胞周期的影响 预览
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作者 王超 刘玉 +3 位作者 安然 赵京山 孙佳欢 李爱英 《中国药理学通报》 CAS CSCD 北大核心 2019年第3期376-381,共6页
目的观察Roscovitine对TNF-α诱导的大鼠血管平滑肌细胞(VSMC)增殖及细胞周期的影响。方法组织贴块法培养大鼠VSMC细胞,采用TNF-α诱导其增殖,加入不同浓度的Roscovitine预处理15 h,将细胞分为:对照组、TNF-α组、Roscovitine 5、10、15... 目的观察Roscovitine对TNF-α诱导的大鼠血管平滑肌细胞(VSMC)增殖及细胞周期的影响。方法组织贴块法培养大鼠VSMC细胞,采用TNF-α诱导其增殖,加入不同浓度的Roscovitine预处理15 h,将细胞分为:对照组、TNF-α组、Roscovitine 5、10、15、30μmol·L-1组。MTT比色法检测细胞增殖活性;Western blot检测增殖细胞核抗原(PCNA);流式细胞仪检测细胞周期;荧光定量RT-PCR及Western blot检测细胞周期蛋白(Cyclin A、Cyclin B、Cyclin D、Cyclin E)、细胞周期蛋白依赖激酶(CDK4、CDK5)、细胞周期抑制蛋白(p53、p21、p27)的表达。结果Roscovitine能抑制VSMC增殖;抑制细胞周期从G 0/G 1期向S期转化。与TNF-α组比较,Roscovitine 5、10、15、30μmol·L^-1组能降低细胞周期蛋白Cyclin A、Cyclin B、Cyclin D、Cyclin E蛋白表达,降低细胞周期蛋白依赖激酶CDK4、CDK5蛋白表达,升高细胞周期抑制蛋白p53、p21、p27蛋白表达(P<0.05)。结论Roscovitine可抑制大鼠VSMC细胞周期进程及增殖活性。 展开更多
关键词 ROSCOVITINE 血管平滑肌细胞 增殖 细胞周期 细胞周期蛋白 细胞周期蛋白依赖性激酶 细胞周期抑制蛋白
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细胞周期蛋白在精子发生过程中的作用
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作者 蔡滨 孙大林 +1 位作者 邓伟民(综述) 金保方(审校) 《中华男科学杂志》 CAS CSCD 北大核心 2019年第2期168-171,共4页
精子发生是一个复杂而又精确的生殖细胞分化的过程,它经历了精原细胞有丝分裂、精母细胞减数分裂和精子形成3个阶段。这一过程受许多因素的调控,其中细胞周期蛋白的调节起着决定性作用。细胞周期蛋白通过与相应的细胞周期蛋白依赖激酶... 精子发生是一个复杂而又精确的生殖细胞分化的过程,它经历了精原细胞有丝分裂、精母细胞减数分裂和精子形成3个阶段。这一过程受许多因素的调控,其中细胞周期蛋白的调节起着决定性作用。细胞周期蛋白通过与相应的细胞周期蛋白依赖激酶结合形成具有蛋白激酶活性的异源二聚体复合物,磷酸化多种蛋白,推动细胞周期各时相的有序进行。近年来研究发现,细胞周期蛋白A、B、D、E在精子发生的调控中扮演着重要角色,本文综述了这4种细胞周期蛋白在调控精子发生中的作用。 展开更多
关键词 细胞周期蛋白 细胞周期蛋白依赖激酶 精子发生
联合检测Sp1、KLF8、Cyclin D1及P16在卵巢浆液性肿瘤中的表达及预后意义 预览
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作者 顾静凤 陈芳 +2 位作者 张菊平 高小姣 邓志勇 《中国社区医师》 2019年第23期127-128,共2页
目的:探讨卵巢浆液性肿瘤中Sp1、KLF8、Cyclin D1及P16蛋白表达的差异和临床意义。方法:采用免疫组化SP法检测卵巢浆液性囊腺癌50例、卵巢浆液性囊腺瘤20例和卵巢交界性浆液性囊腺瘤20例组织中Sp1、KLF8、Cyclin D1及P16的表达情况,并... 目的:探讨卵巢浆液性肿瘤中Sp1、KLF8、Cyclin D1及P16蛋白表达的差异和临床意义。方法:采用免疫组化SP法检测卵巢浆液性囊腺癌50例、卵巢浆液性囊腺瘤20例和卵巢交界性浆液性囊腺瘤20例组织中Sp1、KLF8、Cyclin D1及P16的表达情况,并分析其与临床病理特征之间的相关性。结果:在卵巢浆液性囊腺癌、交界性浆液性囊腺瘤和浆液性囊腺瘤中,Sp1的阳性表达率分别为68.0%、25.0%和15.0%;KLF8分别为64.0%、40.0%和10.0%;Cyclin D1分别为48.0%、80.0%和20.0%;P16分别为18.0%、50.0%和70.0%。Sp1和P16的阳性表达率在卵巢浆液性囊腺癌与交界性浆液性囊腺瘤或浆液性囊腺瘤相比,差异有统计学意义(P<0.05);KLF8和Cyclin D1的阳性表达率在三组间比较,差异有统计学意义(P<0.05)。结论:Sp1、KLF8及Cyclin D1在卵巢浆液性囊腺癌中高表达,而P16在卵巢浆液性囊腺癌中低表达,提示这4种基因的异常表达可能参与了卵巢浆液性囊腺癌的发生发展。 展开更多
关键词 卵巢浆液性肿瘤 SP1 KLF8 CYCLIN D1 P16
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C-X-C型趋化因子受体4在散发性恶性外周神经鞘瘤患者中的预后价值 预览
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作者 Chao Zhang Fang-Yuan Chang +1 位作者 Wen-Ya Zhou Ji-Long Yang 《癌症》 SCIE CAS CSCD 2019年第1期1-9,共9页
背景与目的最近研究表明C-X-C型趋化因子受体4(C-X-C motif chemokine receptor 4,CXCR4)及其配体C-X-C型趋化因子配体12(C-X-C motif chemokine ligand 12,CXCL12)刺激1型神经纤维瘤相关恶性外周神经鞘瘤(malignant peripheral nerve s... 背景与目的最近研究表明C-X-C型趋化因子受体4(C-X-C motif chemokine receptor 4,CXCR4)及其配体C-X-C型趋化因子配体12(C-X-C motif chemokine ligand 12,CXCL12)刺激1型神经纤维瘤相关恶性外周神经鞘瘤(malignant peripheral nerve sheath tumor,MPNST)细胞中细胞周期调控蛋白Cyclin D1的表达,并促进其增殖。在本研究中,我们测定了中国患者的散发性MPNST组织中CXCR4、CXCL12和Cyclin D1蛋白的表达,并探讨了它们的预后价值。方法使用免疫组化染色对58例中国散发性MPNST患者样本中的CXCR4、CXCL12和Cyclin D1蛋白表达水平进行评价。采用Kaplan-Meier分析和log-rank检验评价它们的预后价值。采用多因素Cox回归分析确定独立预后因素。结果分别在19(32.8%)例、32(55.2%)例和16(27.6%)例样本中观察到CXCR4、CXCL12和Cyclin D1的高表达。CXCR4表达与CXCL12表达(r=0.334,P=0.010)和Cyclin D1表达(r=0.309,P=0.018)呈正相关。与CXCR4低表达的患者相比,CXCR4高表达的患者总生存期更长(χ~2=4.642,P=0.031)。结论对于散发性MPNST,CXCR4高表达可能表征着其中一种预后较好的特殊亚型。 展开更多
关键词 散发性恶性外周神经鞘瘤 C-X-C型趋化因子受体4(CXCR4) C-X-C型趋化因子配体12(CXCL12) CYCLIN D1
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NIMA related kinase 2 promotes gastric cancer cell proliferation via ERK/MAPK signaling 预览
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作者 Wei-Dong Fan Tao Chen Peng-Jun Liu 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第23期2898-2910,共13页
BACKGROUND NIMA related kinase 2(NEK2) is closely related to mitosis, and it is currently considered to be over-expressed frequently in many poorly prognostic cancers.However, the effect of the up-regulated NEK2 on ce... BACKGROUND NIMA related kinase 2(NEK2) is closely related to mitosis, and it is currently considered to be over-expressed frequently in many poorly prognostic cancers.However, the effect of the up-regulated NEK2 on cellular signaling in tumors,such as gastric cancer(GC), is con-fusing.AIM To determine the role of the up-regulation of NEK2 in GC.METHODS To investigate the pathological significance of NEK2 in GC, the expression pattern of NEK2 in GC was investigated based on the 'Oncomain' database and compared between 30 pairs of cancer samples and adjacent tissues. The coexpression of NEK2 and ERK in GC was analyzed using The Cancer Genome Atlas(TCGA) database and confirmed in clinical samples by quantitative realtime PCR(qRT-PCR), and the survival curve was also plotted. Western blot or qRT-PCR was used to analyze the effect of NEK2 on the phosphorylation levels of ERK and c-JUN in two GC cell lines(BGC823 and SGC7901) with NEK2 overexpression, and the expression of the downstream effector cyclin D1.Furthermore, CCK8, EdU incorporation assay, and flow cytometry were used to detect the proliferative ability of BGC823 and SGC7901 cells with stably silenced ERK.RESULTS NEK2 was significantly up-regulated in human GC tissues. ERK was significantly associated with NEK2 expression in human clinical specimens, and combined overexpression of NEK2 and ERK potentially forecasted a poor prognosis andsurvival in GC patients. NEK2 knockdown in GC cells inhibited ERK and c-JUN phosphory-lation and reduced the transcription of cyclin D1. More interestingly,NEK2 can rescue the inhibition of cellular viability, proliferation, and cell cycle progression due to ERK knockdown.CONCLUSION Our results indicate that NEK2 plays a carcinogenic role in the malignant proliferation of GC cells via the ERK/MAPK signaling, which may be important for treatment and improving patient survival. 展开更多
关键词 NIMA RELATED KINASE 2 ERK/MAPK SIGNALING Cyclin D1 Cell proliferation Gastric cancer
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Cyclin Cyc2p is required for micronuclear bouquet formation in Tetrahymena thermophila
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作者 Jing Xu Xiaoxiong Li +2 位作者 Weibo Song Wei Wang Shan Gao 《中国科学:生命科学英文版》 SCIE CAS CSCD 2019年第5期668-680,共13页
Meiotic bouquet formation(known as crescent formation in Tetrahymena thermophila) is indispensable for homologous pairing and recombination, but the regulatory mechanism of bouquet formation remains largely unknown. A... Meiotic bouquet formation(known as crescent formation in Tetrahymena thermophila) is indispensable for homologous pairing and recombination, but the regulatory mechanism of bouquet formation remains largely unknown. As a conjugation specific cyclin gene, CYC2 knockout mutants failed to form an elongated crescent structure and aborted meiosis progress in T. thermophila. γ-H2 A.X staining revealed fewer micronuclear DNA double-strand breaks(DSBs) in cyc2Δ cells than in wild-type cells. Furthermore, cyc2Δ cells still failed to form a crescent structure even though DSBs were induced by exogenous agents,indicating that a lack of DSBs was not completely responsible for failure to enter the crescent stage. Tubulin staining showed that impaired perinuclear microtubule structure may contribute to the blockage in micronuclear elongation. At the same time,expression of microtubule-associated kinesin genes, KIN11 and KIN141, was significantly downregulated in cyc2Δ cells.Moreover, micronuclear specific accumulation of heterochromatin marker trimethylated H3 K23 abnormally increased in the cyc2Δ mutants. Together, these results show that cyclin Cyc2 p is required for micronuclear bouquet formation via controlling microtubule-directed nuclear elongation in Tetrahymena. 展开更多
关键词 TETRAHYMENA MEIOSIS CYCLIN Cyc2p bouquet FORMATION
Brucea javanica oil emulsion improves the effect of radiotherapy on esophageal cancer cells by inhibiting cyclin D1-CDK4/6 axis 预览
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作者 Zhong-Hua Qiu Wei-Wei Zhang +1 位作者 Hong-Hua Zhang Gui-Hua Jiao 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第20期2463-2472,共10页
BACKGROUND Esophageal cancer is one of the most common cancers around the world, and it has high incidence and mortality rates. The conventional therapy for esophageal cancer is radiotherapy, although its effect is hi... BACKGROUND Esophageal cancer is one of the most common cancers around the world, and it has high incidence and mortality rates. The conventional therapy for esophageal cancer is radiotherapy, although its effect is highly limited by the resistance of esophageal cancer cells. Thus, strong radiosensitizers can be very crucial during radiotherapy against esophageal cancer. Brucea javanica oil emulsion (BJOE) is a widely used drug against various cancers, such as liver, colon, and ovarian cancer. However, its anti-cancer effect and mechanism and the use of BJOE as a radiosensitizer have not been explored in esophageal cancer. AIM To evaluate the anti-cancer effect and mechanism of BJOE and explore the potential use of BJOE as a radiosensitizer during radiotherapy. METHODS The inhibitory effect of BJOE and its enhancement function with radiation on cell viability were examined with the calculated half-maximal effective concentration and half-maximal lethal concentration. The influence of BJOE on cell migration and invasion were measured with EC109 and JAR cells by wound-healing and transwell assay. Clonogenesis and apoptotic rate, which was measured by Hoechst staining, were investigated to confirm its enhancement function with radiation. To investigate the molecular pathway underlying the effect of BJOE, the expressions of several apoptosis- and cycle-related proteins was detected by western blotting.cell lines more than normal cell lines, and it markedly reduced migration and invasion in esophageal cancer cells (EC109 and JAR). Moreover, it promoted cell apoptosis and enhanced the effect of radiotherapy against esophageal cancerous cells. In the viability test, the values of half-maximal effective concentration and half-maximal lethal concentration were reduced. Compared to the control, only around 1/5 colonies formed when using BJOE and radiation together in the clonogenic assay. The apoptotic rate in EC109 was obviously promoted when BJOE was added during radiotherapy. Our study suggests that the expression of the 展开更多
关键词 ESOPHAGEAL cancer Brucea JAVANICA oil EMULSION RADIOSENSITIZER Apoptosis Cyclin D1-CDK4/6 AXIS
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非小细胞肺癌中Hath1、p27和Cyclin D1的表达及临床意义 预览
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作者 梁彩霞 吴华 +2 位作者 支梅芬 陈满瑜 庞雅君 《临床医学工程》 2019年第3期304-306,共3页
目的检测Hath1、细胞激酶抑制物p27和细胞周期素D1 (Cyclin D1)在非小细胞肺癌组织中的表达,分析其相互关系及其与临床病理参数的关系,了解其在非小细胞肺癌发生、发展中的作用。方法采用免疫组织化学法和实时荧光定量逆转录聚合酶链反... 目的检测Hath1、细胞激酶抑制物p27和细胞周期素D1 (Cyclin D1)在非小细胞肺癌组织中的表达,分析其相互关系及其与临床病理参数的关系,了解其在非小细胞肺癌发生、发展中的作用。方法采用免疫组织化学法和实时荧光定量逆转录聚合酶链反应(RT-PCR)法分别从蛋白和mRNA的水平检测非小细胞肺癌组织和癌旁组织中Hath1、p27和Cyclin D1的表达水平,并结合收集的临床病理资料如病理分型、分化程度和淋巴结转移情况进行分析。结果 Hath1、p27在非小细胞肺癌组织中的阳性表达低于癌旁组织, Cyclin D1在非小细胞肺癌组织中的阳性表达高于癌旁组织(P均<0.05)。三者的表达均与肿瘤分化程度和淋巴结转移有关。Hath1在蛋白和基因水平的表达与p27呈明显正相关(P <0.05),与Cyclin D1呈明显负相关(P <0.05);而p27在蛋白和基因水平的表达与Cyclin D1呈明显负相关(P <0.05)。结论 Hath1、p27和Cyclin D1可能参与了非小细胞肺癌的分化、侵袭转移等生物学行为,可作为判断非小细胞肺癌的生物学指标。Hath1、p27和Cyclin D1存在相关性,但具体作用机制还有待进一步研究。 展开更多
关键词 表达 临床意义 非小细胞肺癌 Hath1 P27 CYCLIN D1
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Analysis of cyclin E co-expression genes reveals nuclear transcription factor Y subunit alpha is an oncogene in gastric cancer
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作者 Liang-Yu Bie Dan Li +15 位作者 Yu Mu Sheng Wang Bei-Bei Chen Hui-Fang Lyu Li-Li Han Cai-Yun Nie Chang-Cheng Yang Lin Wang Chuan-Chuan Ren Wei-Jie Zhang Ping Guo Feng Shi Qing-Xia Fan Liu-Xing Wang Xiao-Bing Chen Su-Xia Luo 《慢性疾病与转化医学:英文版》 CSCD 2019年第1期44-52,共9页
Objective: To explore genes potentially co-expressed with cyclin E in gastric cancer and discover possible targets for gastric cancer treatment. Methods: The Cancer Genome Atlas (TCGA) stomach adenocarcinoma sequencin... Objective: To explore genes potentially co-expressed with cyclin E in gastric cancer and discover possible targets for gastric cancer treatment. Methods: The Cancer Genome Atlas (TCGA) stomach adenocarcinoma sequencing data were used to predict genes co-expressed with cyclin E. Co-expression genes predicted by cBioPortal online analysis with Pearson correlation coefficient ≥0.4 were analyzed by gene ontology (GO) enrichment annotation using the PANTHER online platform (Ver. 7). Interactions between proteins encoded by these genes were analyzed using the STRING online platform (Ver. 10.5) and Cytoscape software (Ver. 3.5.1). Genes displaying a high degree of connection were analyzed by transcription factor enrichment prediction using FunRich software (Ver. 3). The significant transcription factor and cyclin E expression levels and their impact on gastric cancer progression were analyzed by Western blotting and Kaplan-Meier survival curve analysis. Results: After filtering the co-expression gene prediction results, 78 predicted genes that included 73 protein coding genes and 5 non-coding genes with Pearson correlation coefficient ≥0.4 were selected. The expressions of the genes were considered to be correlated with cyclin E expression. Among the 78 genes co-expressed with cyclin E, 19 genes at the central of the regulatory network associated with cyclin E were discovered. Nuclear transcription factor Y subunit alpha (NF-YA) was identified as a significant transcription factor associated with cyclin E co-expressing genes. Analysis of specimen donors’ clinical records revealed that high expression of NF-YA tended to be associated with increased cyclin E expression. The expression of both was associated with progression of gastric cancer. Western blotting results showed that compared with normal tissues, NF-YA and cyclin E were highly expressed in tumor tissues (P < 0.001). Survival curve analysis clearly demonstrated relatively poor overall survival of gastric cancer patients with high cyclin E or high NF-YA ex 展开更多
关键词 Cyclin E NUCLEAR transcription factor Y SUBUNIT alpha ONCOGENE Gastric cancer
肝再生磷酸酶-2的分子结构与功能研究进展
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作者 杜新月 吴琛耘 王兆军 《生命科学》 CSCD 北大核心 2019年第7期693-698,共6页
肝再生磷酸酶-2 (PRL-2)在机体正常的细胞、组织和器官,以及大部分的肿瘤中普遍高表达,以其致癌原性被广泛认知,但分子机制尚不明确。近几年的研究发现了PRL-2的新型结合伴侣--细胞周期蛋白M (cyclin-M, CNNM)家族,为进一步解析PRL-2的... 肝再生磷酸酶-2 (PRL-2)在机体正常的细胞、组织和器官,以及大部分的肿瘤中普遍高表达,以其致癌原性被广泛认知,但分子机制尚不明确。近几年的研究发现了PRL-2的新型结合伴侣--细胞周期蛋白M (cyclin-M, CNNM)家族,为进一步解析PRL-2的生理生物功能提供新的方向。现总结PRL-2分子在实体瘤、血液肿瘤中的作用,探讨其结构与功能的关系,并对PRL-2及其结合伴侣的生理功能进行全面综述。 展开更多
关键词 肝再生磷酸酶-2 肿瘤 细胞周期蛋白M 生理功能
PRMT2β suppresses autophagy and glycolysis pathway in human breast cancer MCF-7 cell lines
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作者 Yajun Chenx Xianpeng Dai +7 位作者 Yao Yao Jing Wang Xinzhi Yang Yunsheng Zhang Jing Yang Renxian Cao Gebo Wen Jing Zhong 《生物化学与生物物理学报:英文版》 SCIE CAS CSCD 2019年第3期335-337,共3页
Autophagy as a novel therapeutic target can inhibit or increase treatment efficacy in various types of breast cancer in a cell-type-dependent manner [1,2].Several studies have revealed that the coordination between Ak... Autophagy as a novel therapeutic target can inhibit or increase treatment efficacy in various types of breast cancer in a cell-type-dependent manner [1,2].Several studies have revealed that the coordination between Akt and the glycolytic pathway plays an indispensable role in mediating autophagy and caspase-dependent apoptosis,suggesting that a new regulatory mechanism for the process [3,4].Protein arginine N-methyltransferases(PRMTs)are eukaryotic enzymes that catalyze the transfer of methyl groups from S-adenosylmethionine to arginine residues of numerous PRMT substrates [5,6].PRMT2(also known as HRMT1L1)belongs to the arginine methyltransferase family [7].PRMT2β is a novel PRMT2 splice variant isolated from breast cancer cell [8].It occurs at the 3′ end of the PRMT2,resulting in loss of exons 7–9 and downstream frame-shifting [9].PRMT2β possesses 83 new amino acids at the C-terminus and its size is 301 amino acids.Our previous study reported that PRMT2β has potential antitumor effect by suppressing cyclin D1 expression [10].However,little is known about whether PRMT2β could regulate autophagy and glycolysis of MCF-7 cells. 展开更多
关键词 MCF-7 apoptosis expression treatment CYCLIN can Akt its
Battle of the sexes: contrasting roles of testis-specific protein Y-encoded (TSPY) and TSPX in human oncogenesis
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作者 Yun-Fai Chris Lau Yunmin Li Tatsuo Kido 《亚洲男性学杂志:英文版》 SCIE CAS CSCD 2019年第3期260-269,共10页
The Y-located testis-specific protein Y-encoded (TSPY) and its X-homologue TSPX originated from the same ancestral gene, but act as a proto-oncogene and a tumor suppressor gene, respectively. TSPY has specialized in m... The Y-located testis-specific protein Y-encoded (TSPY) and its X-homologue TSPX originated from the same ancestral gene, but act as a proto-oncogene and a tumor suppressor gene, respectively. TSPY has specialized in male-specific functions, while TSPX has assumed the functions of the ancestral gene. Both TSPY and TSPX harbor a conserved SET/NAP domain, but are divergent at flanking structures. Specifically, TSPX contains a C-terminal acidic domain, absent in TSPY. They possess contrasting properties, in which TSPY and TSPX, respectively, accelerate and arrest cell proliferation, stimulate and inhibit cyclin B-CDK1 phosphorylation activities, have no effect and promote proteosomal degradation of the viral HBx oncoprotein, and exacerbate and repress androgen receptor (AR) and constitutively active AR variant, such as AR-V7, gene transactivation. The inhibitory domain has been mapped to the carboxyl acidic domain in TSPX, truncation of which results in an abbreviated TSPX exerting positive actions as TSPY. Transposition of the acidic domain to the C-terminus of TSPY results in an inhibitory protein as intact TSPX. Hence, genomic mutations/aberrant splicing events could generate TSPX proteins with truncated acidic domain and oncogenic properties as those for TSPY. Further, TSPY is upregulated by AR and AR-V7 in ligand-dependent and ligand-independent manners, respectively, suggesting the existence of a positive feedback loop between a Y-located proto-oncogene and male sex hormone/receptors, thereby amplifying the respective male oncogenic actions in human cancers and diseases. TSPX counteracts such positive feedback loop. Hence, TSPY and TSPX are homologues on the sex chromosomes that function at the two extremes of the human oncogenic spectrum. 展开更多
关键词 ANDROGEN receptor AR-V7 cell cycle regulation cyclin B-CDK1 oncogene SEX chromosomes SEX differences TSPX TSPY tumor suppressor
幽门螺旋杆菌感染性胃癌组织中Cyclin D1、MMP-9的表达及意义 预览 被引量:2
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作者 徐文敏 张海燕 +1 位作者 何苗 谭婧宇 《临床与实验病理学杂志》 CAS CSCD 北大核心 2019年第2期213-215,共3页
目的探讨幽门螺旋杆菌(helicobacter pylori,HP)感染性胃癌组织中Cyclin D1、MMP-9的表达及临床意义。方法收集重庆市肿瘤医院手术切除的胃癌组织104例,分为HP阳性组(n=56)和HP阴性组(n=48),另收集40例癌旁正常胃组织作为对照组。采用... 目的探讨幽门螺旋杆菌(helicobacter pylori,HP)感染性胃癌组织中Cyclin D1、MMP-9的表达及临床意义。方法收集重庆市肿瘤医院手术切除的胃癌组织104例,分为HP阳性组(n=56)和HP阴性组(n=48),另收集40例癌旁正常胃组织作为对照组。采用免疫组化SP法检测各组中Cyclin D1和MMP-9蛋白的表达,分析两者表达与胃癌临床病理特征、HP感染的关系及其对预后的影响。结果 HP阳性组Cyclin D1和MMP-9阳性率高于HP阴性组和对照组,差异有统计学意义(P <0. 017);临床分期Ⅲ+Ⅳ期、分化程度低、有淋巴结转移者的Cyclin D1和MMP-9阳性率明显高于临床分期Ⅰ+Ⅱ期、分化程度高、无淋巴结转移者(P <0. 05);Cyclin D1和MMP-9均与HP感染呈正相关(rs=0. 504,rs=0. 532,P均<0. 05);HP阳性、Cyclin D1和MMP-9阳性者生存期均显著较短(P均<0. 05)。结论 Cyclin D1和MMP-9在HP感染性胃癌组织中表达上调,与HP共同促进胃癌的发生、发展,影响预后。 展开更多
关键词 胃肿瘤 幽门螺旋杆菌 CYCLIN D1 MMP-9 免疫组织化学
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活血散结方药物血浆对PDGF干预下兔视网膜色素上皮细胞增殖相关因子及ERK1/2信号转导通路的影响 预览
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作者 刘晓清 彭俊 +4 位作者 张又玮 谭涵宇 李建超 吴权龙 彭清华 《中国中医药信息杂志》 CAS CSCD 2019年第2期62-68,共7页
目的观察活血散结方对血小板源性生长因子(PDGF)干预下兔视网膜色素上皮(RPE)细胞增殖的影响,探讨其分子机制。方法以RPE细胞为研究对象,体外进行RPE细胞原代培养及传代,建立PDGF干预下兔RPE细胞增殖模型,并选取PDGF最佳干预浓度。制备... 目的观察活血散结方对血小板源性生长因子(PDGF)干预下兔视网膜色素上皮(RPE)细胞增殖的影响,探讨其分子机制。方法以RPE细胞为研究对象,体外进行RPE细胞原代培养及传代,建立PDGF干预下兔RPE细胞增殖模型,并选取PDGF最佳干预浓度。制备活血散结方药物血浆,对兔RPE细胞增殖与毒性的影响进行测定,选取最佳的实验浓度。实验分为空白对照组(DMEM)、正常血浆组、PDGF(10μg/L)组、PDGF(10μg/L)+AG1296(10μmol/L)组、PDGF(10μg/L)+10%药物血浆组、PDGF(10μg/L)+20%药物血浆组。分别加入相应处理因素干预48 h,Transwell流式细胞仪检测细胞周期变化;Western blot检测兔RPE细胞cyclinD1蛋白、pERK1/2及ERK1/2蛋白表达。结果 10%、20%活血散结方药物血浆对RPE细胞周期、cyclin D1蛋白及p ERK1/2、ERK1/2蛋白有抑制作用,与AG1296抑制剂作用相当(P>0.05),与其他组比较差异均有统计学意义(P<0.01)。结论活血散结方药物血浆可能通过影响ERK信号通路及对周期蛋白的调控,抑制PDGF干预下兔RPE细胞增殖。 展开更多
关键词 活血散结方 药物血浆 视网膜色素上皮细胞 细胞周期 血小板源性生长因子 cyclin D1蛋白 pERK1/2蛋白 ERK1/2蛋白
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名医验方加味黄芪建中汤对慢性萎缩性胃炎大鼠胃黏膜细胞增殖相关蛋白的调控 预览
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作者 张锐 曹瑞岗 +3 位作者 李永乐 郭志娟 孔令惠 钱占红 《江西中医药》 2019年第5期66-69,共4页
目的:探讨加味黄芪建中汤对慢性萎缩性胃炎(CAG)模型大鼠胃黏膜细胞增殖的影响及机制。方法:选择SPF级SD大鼠48只,随机分为正常组、模型组、维酶素阳性对照组、加味黄芪建中汤低、中、高剂量组,每组8只,采用自由饮用MNNG复制CAG大鼠模型... 目的:探讨加味黄芪建中汤对慢性萎缩性胃炎(CAG)模型大鼠胃黏膜细胞增殖的影响及机制。方法:选择SPF级SD大鼠48只,随机分为正常组、模型组、维酶素阳性对照组、加味黄芪建中汤低、中、高剂量组,每组8只,采用自由饮用MNNG复制CAG大鼠模型,运用HE染色观察胃黏膜组织病理形态学改变,运用Western blot检测胃黏膜组织中PCNA、Cyclin E表达及其含量。结果:加味黄芪建中汤可有效治疗模型大鼠的CAG。与模型组、维酶素阳性对照组相比,加味黄芪建中汤能够降低胃黏膜组织中PCNA、Cyclin E蛋白表达,差异具有统计学意义(P<0.05或P<0.01)。结论:加味黄芪建中汤通过降低CAG模型大鼠胃黏膜中PCNA、Cyclin E的表达水平,抑制G1/S期的转换,能够调节胃黏膜细胞的异常增殖,可能是治疗CAG的关键机制之一。 展开更多
关键词 慢性萎缩性胃炎 加味黄芪建中汤 细胞增殖 PCNA CYCLIN E
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2,3,7,8-Tetrachlorodibenzo-p-dioxin Promotes Proliferation of Astrocyte Cells via the Akt/STAT3/Cyclin D1 Pathway 预览
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作者 WU Chang Yue YIN Kai Zhi +3 位作者 ZHANG Yan JIAO Man ZHAO Xin Yuan WU Qi Yun 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第4期281-290,共10页
Objective The compound 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD), a persistent organic pollutant, is harmful to the nervous system, but its effects on the brain are still unclear. This study aimed to investigate the e... Objective The compound 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD), a persistent organic pollutant, is harmful to the nervous system, but its effects on the brain are still unclear. This study aimed to investigate the effects of TCDD on astrocytes proliferation and underlying molecular mechanism. Methods The cell proliferation was measured by EdU-based proliferation assay and PI staining by flow cytometry. Protein expression levels were detected by Western blotting. Immunofluorescence, cytoplasmic and nuclear fractions separation were used to assess the distribution of signal transducer and activator of transcription 3(STAT3). Results C6 cells treated with 10 and 50 nmol/L TCDD for 24 h showed significant promotion of the proliferation of. The exposure to TCDD resulted in the upregulation in the expression levels of phosphorylated protein kinase B(p-Akt), phosphorylated STAT3, and cyclin D1 in a dose-and time-dependent manner. The inhibition of Akt expression with LY294002 or STAT3 expression with AG490 abolished the TCDD-induced cyclin D1 upregulation and cell proliferation. Furthermore, LY294002 suppressed the activation of STAT3. Finally, TCDD promoted the translocation of STAT3 from the cytoplasm to the nucleus, and LY294002 treatment blocked this effect. Conclusion TCDD exposure promotes the proliferation of astrocyte cells via the Akt/STAT3/cyclin D1 pathway, leading to astrogliosis. 展开更多
关键词 2 3 7 8-tetrachlorodibenzo-p-dioxin (TCDD) AKT STAT3 Cyclin D1 PROLIFERATION Astrocytes
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Mechanisms of microRNA-150, cyclin B1 and mitochondrial-associated protein 2 in regulating apoptosis and inhibiting invasion and migration of Huh-7 hepatocellular carcinoma cells
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作者 Feng Wen Yan Xiang 《海南医科大学学报(英文版)》 2019年第9期11-14,共4页
Objective: To explore the mechanisms of microRNA-150, cyclin B1 and mitochondrial-associated protein 2 in regulating the apoptosis and inhibiting the invasion and migration of Huh-7 cells. Methods: Huh-7 cells were di... Objective: To explore the mechanisms of microRNA-150, cyclin B1 and mitochondrial-associated protein 2 in regulating the apoptosis and inhibiting the invasion and migration of Huh-7 cells. Methods: Huh-7 cells were divided into the control group, the negative control group (NC group) and the miR-150 overexpression group (mimic group). The miR-150 overexpressing cell line was constructed by plasmid transfection. The cell viability and apoptosis were detected by cell counting kit-8 and flow cytometry. The cell migration and invasion capacity were measured by cell wound scratch assay and Transwell. The levels of miRNA and mRNA were detected by real-time quantitative polymerase chain reaction and the relative expression levels of proteins were detected by Western blot. Results: MiR-150 significantly inhibited the cell viability of Huh-7 and promoted its apoptosis (P<0.01). After 24 h of cultivation, the mobility of the control group and the NC group were (83.54±4.66)%and (85.57±4.74)%, respectively. The mobility of the mimic group was (49.63±3.78)%, which was significantly lower than that of the control group and the NC group (P<0.01). After 24 h of cultivation, the invasive rate of the control group and the NC group were (100.56±2.87)%and (101.63±3.74)%, respectively, and the invasive rate of mimic group was (51.63±5.32)%, which was significantly lower than that of the control group and the NC group (P<0.01). The expression levels of cyclin B1 protein and mRNA in the mimic group were significantly lower than those in the control group and the NC group (P<0.01), and the level of mitochondrial-associated protein 2 in the mimic group was significantly higher than that in the control group and the NC group (P<0.01). Conclusions: MiR-150 may inhibit the proliferation, migration, invasion and apoptosis of hepatoma carcinoma cell by regulating cyclin B1 or up-regulating mitochondrial-associated protein 2 levels. 展开更多
关键词 Liver cancer MiR-150 CYCLIN B1 MITOCHONDRIAL fusion protein 2
过表达PTEN基因对甲状腺髓样癌细胞生长及细胞周期的影响 预览
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作者 翟明慧 袁殿宝 +1 位作者 赵峻峰 张敬 《临床与病理杂志》 2019年第3期464-469,共6页
目的:探讨PTEN基因对甲状腺髓样癌(medullary thyroid carcinoma,MTC)细胞生长、周期的影响以及作用机制。方法:使用酶切酶连的方法构建真核表达载体pcDNA3.1-PTEN,采用脂质体转染法将其转入pA10RP8细胞中。实时荧光定量PCR(real-time q... 目的:探讨PTEN基因对甲状腺髓样癌(medullary thyroid carcinoma,MTC)细胞生长、周期的影响以及作用机制。方法:使用酶切酶连的方法构建真核表达载体pcDNA3.1-PTEN,采用脂质体转染法将其转入pA10RP8细胞中。实时荧光定量PCR(real-time quantitative polymerase chain reaction,RT-qPCR)检测细胞内PTEN表达水平的变化,Western印迹法检测PTEN,Ki-67,p21,cyclinD1蛋白表达水平的变化;CCK-8法和平板克隆试验检测PTEN对pA10RP8细胞增殖的影响;细胞流式术检测pA10RP8细胞周期的变化。结果:成功构建了PTEN真核表达载体。转染pcDNA3.1-PTEN质粒的pA10RP8细胞PTEN和p21表达上调,Ki-67和cyclin D1蛋白表达下调;转染pcDNA3.1-PTEN质粒的pA10RP8细胞生长增殖受到抑制;细胞在G0/G1期比例增加,发生周期阻滞。结论:过表达PTEN能够抑制pA10RP8细胞的增殖,诱导细胞周期阻滞,其机制可能与周期蛋白p21上调和cyclin D1下调表达有关。 展开更多
关键词 甲状腺髓样癌 PTEN 细胞周期 细胞周期蛋白
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左归丸含药血清对大鼠卵巢颗粒细胞Smad4及周期蛋白CyclinA表达的影响 预览
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作者 赵粉琴 王馨怡 +2 位作者 李娜娜 王新斌 刘凯 《中国中医药科技》 CAS 2018年第2期180-183,共4页
目的:探讨Smad4蛋白对大鼠卵巢颗粒细胞细胞周期蛋白cyclinA的影响以及左归丸的干预作用。方法:以不同剂量的左归丸含药血清作用于体外培养的大鼠卵巢颗粒细胞,用MTS法检测各组颗粒细胞24、48、72h增殖情况以及用Western bolt法检测... 目的:探讨Smad4蛋白对大鼠卵巢颗粒细胞细胞周期蛋白cyclinA的影响以及左归丸的干预作用。方法:以不同剂量的左归丸含药血清作用于体外培养的大鼠卵巢颗粒细胞,用MTS法检测各组颗粒细胞24、48、72h增殖情况以及用Western bolt法检测卵巢颗粒细胞Smad4和cyclinA蛋白表达。结果:左归丸含药血清对大鼠卵巢颗粒细胞的增殖有促进作用,在作用48h,2.5%左归丸含药血清组作用最为显著(P〈0.05);同时Smad4和cyclinA蛋白也达到最大表达(P〈0.05)。结论:左归丸含药血清对卵巢颗粒细胞增殖的促进作用与增加Smad4信号通路细胞周期蛋白cyclinA表达,抑制细胞分裂有关。 展开更多
关键词 左归丸含药血清 卵巢颗粒细胞 SMAD4蛋白 细胞周期蛋白(cyclin A) 大鼠
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二甲双胍对小鼠睾丸间质细胞的体外抑制作用及其机制研究 预览
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作者 李蕊秀 赵昍朋 +5 位作者 刘婧 吴家栋 何瑞平 岳鑫 张传领 肖瑞 《实用医学杂志》 北大核心 2018年第16期2634-2637,2643共5页
目的探讨二甲双胍在小鼠睾丸发育中的作用及其分子机制。方法不同浓度二甲双胍作用于小鼠睾丸间质细胞(TM3),采用M1Tr法、Annexin V—FITC/PI法及流式细胞术检测二甲双胍对TM3细胞增殖、凋亡及周期的影响。采用real—timePCR检测细... 目的探讨二甲双胍在小鼠睾丸发育中的作用及其分子机制。方法不同浓度二甲双胍作用于小鼠睾丸间质细胞(TM3),采用M1Tr法、Annexin V—FITC/PI法及流式细胞术检测二甲双胍对TM3细胞增殖、凋亡及周期的影响。采用real—timePCR检测细胞周期关键调节蛋白cvclinB1和cvcli。D1的变化,WesternBlot分析二甲双胍抑制TM3细胞增殖中AMPK蛋白的变化。结果MTT结果显示二甲双孤可显著抑制TM3细胞的体外增殖能力;AnnexinV—FITC/PI染色法显示二甲双胍未诱导该细胞发生凋亡;流式细胞术检测发现二甲双胍可诱导TM3细胞周期阻滞在G2/M期;real—timePCR显示二甲双胍可下调cvclinB1和cyclin D1 mRNA的表达;WesternBlot显示二甲双胍显著增加TM3细胞中AMPK在Thr172位点的磷酸化水平。结论二甲双胍显著抑-NTM3细胞的体外增殖并通过诱导TM3细胞周期阻滞在G2/M期.激活AMPK信号通路,发挥其对TM3细胞的体外抑制作用。 展开更多
关键词 二甲双胍 小鼠睾丸间质细胞 AMPK CYCLIN B1 CYCLIN D1
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