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Role of the Putative Polysaccharide Deacetylase BA1836 from <i>B. anthracis</i>in Spore Development and Germination 预览
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作者 Anastasia Tomatsidou Dimitris Koutsioulis +3 位作者 Dimitris Tzamarias Michael Kokkinidis Waldemar Vollmer Vassilis Bouriotis 《微生物学(英文)》 2019年第8期679-702,共24页
Putative and known polysaccharide deacetylases (PDAs) from B. anthracis have key roles in resistance to host lysozyme, stabilization of the cell wall, biogenesis of peptidoglycan (PG) and for neutral polysaccharide mo... Putative and known polysaccharide deacetylases (PDAs) from B. anthracis have key roles in resistance to host lysozyme, stabilization of the cell wall, biogenesis of peptidoglycan (PG) and for neutral polysaccharide modification and attachment to PG. Here we elucidated the physiological role of the putative PDA BA1836 from B. anthracis. The ba1836 gene was expressed upon entrance into the stationary phase of growth and enhanced during the early stages of sporulation. The Δba1836 knockout strain had normal growth rate, did not exhibit any significant alterations in PG pattern of stationary phase cells and was not sensitive to lysozyme, but showed a defect in cell separation. Strikingly, the Δba1836 mutant strain exhibited a severe delay in spore development although mature spores were ultimately developed and had normal morphology. Additionally, digestion of Δba1836 mutant spore PG with mutanolysin produced an almost identical muropeptide pattern compared to peptidoglycan from wild type spores, although the amount of all muropeptides was significantly reduced. Finally, knockout spores exhibited a lower germination rate. To our knowledge, BA1836 has a unique role, among the presently characterized PDAs from B. anthracis, in spore development and germination. 展开更多
关键词 Bacillus ANTHRACIS POLYSACCHARIDE DEACETYLASES BA1836 Spores GERMINATION Spore Development
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Isolation and Characterization of Endophytic Fungi from Purslane and the Effects of Isolates on the Growth of the Host 预览
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作者 Mandlaa   +5 位作者 Yu Zhang Yongqing Wan Ying Tie Bo Zhang Ruigang Wang Guangxia Wang 《微生物学(英文)》 2019年第5期438-453,共16页
Purslane, a common weed, has been used as food or folk medicine in many countries. The growth, medicinal components and nutrient contents of the plant are closely associated with endophytes, especially endophytic fung... Purslane, a common weed, has been used as food or folk medicine in many countries. The growth, medicinal components and nutrient contents of the plant are closely associated with endophytes, especially endophytic fungi. In this study, the endophytic fungi associated with purslane were isolated, and the effects of the isolates on the host were investigated to lay a foundation for further research and development of purslane resources. The results showed that a total of eight endophytic fungi were isolated from purslane (collected from Hohhot, Inner Mongolia, China), and they belonged to the genera Penicillium (isolates K, N, P, M and I), Chaetomium (isolate J), Fusarium (isolate H) and Petriella (isolate O). Moreover, the growth of purslane was significantly influenced by its endophytic fungi. Isolate M can significantly decrease the germination rate, while J can significantly increase the germination rate of purslane. In addition, H, J and M can significantly increase the bud length of purslane, and the fermentation broth of P has a negative influence on the bud length of purslane. M and I can significantly increase the height, fresh weight and chlorophyll content of purslane due, in part, to the lower pH of the fermentation broth of I and M. 展开更多
关键词 ENDOPHYTIC Fungi Purslane PORTULACA OLERACEA L. GROWTH Promotion GERMINATION
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鸡血藤种子催芽试验研究 预览
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作者 林春兰 严坤麟 +3 位作者 罗万业 温秀凤 谢金兰 范剑明 《林业科技情报》 2019年第2期1-2,7共3页
比较不同成熟度与不同处理方式对鸡血藤种子发芽率的影响。结果表明:成熟果实的种子发芽率为71%,未成熟果实的种子发芽率为32.3%;用温水浸泡处理种子发芽率为73.3%,用直接湿沙贮藏处理种子发芽率为71.0%。因此鸡血藤应采摘成熟果实的种... 比较不同成熟度与不同处理方式对鸡血藤种子发芽率的影响。结果表明:成熟果实的种子发芽率为71%,未成熟果实的种子发芽率为32.3%;用温水浸泡处理种子发芽率为73.3%,用直接湿沙贮藏处理种子发芽率为71.0%。因此鸡血藤应采摘成熟果实的种子,催芽方式为40℃温水浸泡处理后,捞出置于温暖处催芽,或直接湿沙贮藏催芽均可。 展开更多
关键词 鸡血藤 催芽 贮藏 发芽率
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Vulnerability of Sunflower Germination and Metal Translocation under Heavy Metals Contamination 预览
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作者 Rumana Sadiq Nazimah Maqbool +3 位作者 Bader-Un-Nisa &ensp Kauser Parveen Mumtaz Hussain 《美国植物学期刊(英文)》 2019年第5期738-751,共14页
The germination and metal translocation ability of two sunflower seedlings were compared to identify the cultivar differences towards metal contamination at juvenile stage. The heavy metal treatments include: 0, 50, 1... The germination and metal translocation ability of two sunflower seedlings were compared to identify the cultivar differences towards metal contamination at juvenile stage. The heavy metal treatments include: 0, 50, 100, 150 and 200 mM Ni, Cd and Pb applied in sand filled pots to Hysun-33 and FH-533 sunflower. The highest germination percentage (79%) and vigor index were recorded for Hysun-33 with no heavy metal treatment. Pb and Cd treatments reduced the growth attributes of 20 days old seedlings of both the cultivars. The Ni translocation effectively enhanced the shoot and root biomass of Hysun-33. The high concentration of 150 and 200 mM Cd and Pb drastically reduced Mn and K contents, vigor, length and biomass of two sunflower cultivars. Among three of the heavy metals, Cd was found more toxic than Pb and Ni. Roots of 20 days old seedlings of Hysun-33 were able to hold more Cd metal and stop its translocation to epigenous parts. Although 150 and 200 mM Ni effects the germination and vigor of sunflower cultivars more than 50 and 100 mM Ni, it is found less toxic in comparison to Cd and Pb. The Cd accumulation in roots suggests that it is physiologically most active sink for Cd metal while epigenous parts of sunflower cultivars are sink for Pb and Ni metal as shoot of sunflower cultivars accumulates high contents of Pb and Ni. 展开更多
关键词 TRANSLOCATION GERMINATION Heavy Metals CONTAMINATION SUNFLOWER
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萌发条件对绿豆芽中γ-氨基丁酸含量的影响研究 预览
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作者 石磊 刘超 +1 位作者 周柏玲 孟婷婷 《粮食与油脂》 北大核心 2019年第3期50-53,共4页
以中绿2号为试验材料,采用析因试验设计,确定绿豆芽最佳的萌芽条件。结果表明:当浸种温度为40℃、浸种时间为24h,萌发时间为96h和萌发温度为15℃时的条件下,绿豆芽中GABA含量最高,测得的GABA含量为0.408mg/mL;考虑到生产应用,选用萌发... 以中绿2号为试验材料,采用析因试验设计,确定绿豆芽最佳的萌芽条件。结果表明:当浸种温度为40℃、浸种时间为24h,萌发时间为96h和萌发温度为15℃时的条件下,绿豆芽中GABA含量最高,测得的GABA含量为0.408mg/mL;考虑到生产应用,选用萌发温度为20℃,浸种温度为20℃,浸种时间为24h的萌发条件。在此条件下,谷氨酸和谷氨酸钠都可促进绿豆芽中GABA的转化生成,谷氨酸的促进作用高于谷氨酸钠。当谷氨酸的浓度为7.5‰时,绿豆芽中GABA含量最高,为0.467mg/mL。 展开更多
关键词 绿豆 萌发 Γ-氨基丁酸 萌芽条件 GABA
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Impact of Drought Stress during Germination on Antioxidant Capacities and Antioxidant Enzymes Activities of Madura Local Maize (<i>Zea mays</i>) Seeds 预览
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作者 Agnes Rezkyta Herwang Dani Tri Agus Siswoyo 《农业科学(英文)》 2019年第11期1506-1516,共11页
The effect of various polyethylene glycol (PEG) concentrations to induced drought stress on the antioxidant capacities and antioxidant enzyme activities was investigated on Madura local maize during germination. Seed ... The effect of various polyethylene glycol (PEG) concentrations to induced drought stress on the antioxidant capacities and antioxidant enzyme activities was investigated on Madura local maize during germination. Seed samples were treated under 5%, 10%, and 15% of PEG for 6 days. The changes in the antioxidant capacities such as 2,2’-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and hydroxyl radical scavenging;antioxidant enzyme activities including catalase (CAT) and ascorbate peroxidase (APX) and gene expression were observed during seeds germination. The result showed the antioxidants capacities and enzyme activities exhibited a significantly higher level in drought-treated. The most effective level of drought stress to elevate the capabilities and enzyme activity of antioxidant as well as it gene expression was observed at 15% PEG, whereas ABTS scavenging activity was increased 20% over the control, hydroxyl scavenging activity up to 14%, CAT and APX activity increased 4 - 5 times over the control. Along with the presence of antioxidant mechanisms, including the capacities and activities of different types of ABTS+, hydroxyl, CAT, and APX might play important roles during the germination phase and be able to manage reactive oxygen species to the normal level under stress condition. 展开更多
关键词 Drought ANTIOXIDANT GERMINATION MAIZE Polyethylene-Glycol
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Effects of mepiquat chloride on yield and main properties of cotton seed under different plant densities 预览
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作者 ZHAO Wenqing YAN Qiang +5 位作者 YANG Hongkun YANG Xiaoni WANG Leran CHEN Binglin MENG Yali ZHOU Zhiguo 《棉花研究(英文)》 2019年第2期77-86,共10页
Background:Cottonseed oil and protein content as well as germination traits are major indicators of seed quality.However,the responses of these indicators to plant density and mepiquat chloride(MC)are still uncertain.... Background:Cottonseed oil and protein content as well as germination traits are major indicators of seed quality.However,the responses of these indicators to plant density and mepiquat chloride(MC)are still uncertain.To investigate plant density and MC effects on cotto nseed yield and main quality parameters,we con ducted a twoyear field experiment including four plant densities(1.35,2.55,3.75 and 4.95 plants·m^-2)and two closes of MC(0 and 135g·hm^-2)in Dafeng,Jiangsu Province,in 2013 and 2014.Results:The application of MC reduced plant height,fruit branch length and fruiting branch number under different plant densities,resulting in a lower and more compact plant canopy.Cottonseed yield showed a nonlinear increase as plant density increasing and achieved the highest value at 3.75 plants·m^-2,regardless of MC application.No significant interactio ns were found between plant density and MC for cotton seed yield and quality parameters.The 100-seed weight,cottonseed oil content and vigor index significantly decreased as plant density increased,while these parameters significantly increased with MC applying under different plant densities.Seed vigor index was positively correlated with 100-seed weight and seed oil con tent across different plant densities and MC treatments.Conclusions:Thus,application of MC could realize a win-win situation between cottonseed yield and main quality parameters under various densities;and plant density of 3.75 plants·m^-2 combined with 135 g·hm^-2 of MC applying is optimal for high cottonseed yield and quality in this cotton production area. 展开更多
关键词 Mepiquat CHLORIDE PLANT density COTTONSEED YIELD Oil content Seed GERMINATION
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不同小麦品种种子萌发生长、叶片叶绿素含量和荧光动力学特征差异分析 预览
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作者 张辉 张蓓蓓 +2 位作者 景琦 校思泽 武悦萱 《江西农业学报》 CAS 2019年第9期9-15,共7页
采用室内水培法,研究了4个小麦品种种子萌发、生长及幼苗叶片叶绿素含量和荧光动力学特征差异,并对叶绿素荧光参数和叶绿素含量及种子萌发指标的关系进行了分析。结果显示:不同小麦品种间发芽率、发芽势、发芽指数、活力指数和根重均达... 采用室内水培法,研究了4个小麦品种种子萌发、生长及幼苗叶片叶绿素含量和荧光动力学特征差异,并对叶绿素荧光参数和叶绿素含量及种子萌发指标的关系进行了分析。结果显示:不同小麦品种间发芽率、发芽势、发芽指数、活力指数和根重均达到极显著差异,芽重达到显著差异,以九麦2号的发芽率、发芽势、发芽指数、活力指数、根重和芽重最高;不同小麦品种间叶片叶绿素a、叶绿素b和总叶绿素含量均达到显著差异,以九麦2号的叶绿素a、b和总含量较高;以九麦2号的荧光强度最强,宝研85号次之;在叶绿素荧光动力学曲线(OJIP)中,以九麦2号的O、K、J、I、P点荧光强度最强,且标准化后的相对荧光强度K点和J点以陕垦2号最高,I点以九麦2号最高;单位反应中心捕获量、用于传递的能量以九麦2号最高,反应中心用于热耗散的能量和比率以陕垦2号最高;叶片叶绿素荧光参数与种子萌发指标达到极显著正相关关系(P<0.01),与叶绿素含量也达到显著正相关关系。 展开更多
关键词 小麦 种子萌发 叶绿素含量 荧光动力学
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中亚大麦品种萌发期抗旱性筛选与鉴定 预览
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作者 任毅 王仙 +4 位作者 张金汕 聂石辉 吴高明 耿洪伟 方伏荣 《新疆农业科学》 CAS CSCD 北大核心 2019年第5期882-889,共8页
【目的】研究不同基因型大麦萌发期抗旱特性,筛选萌发期抗旱性鉴定关键指标,建立萌发期抗旱性研究数学模型,为抗旱大麦品种培育提供理论指导。【方法】采用人工气候箱内发芽盒培养,用20%PEG8000溶液对吉33等12份中亚引进大麦品种进行萌... 【目的】研究不同基因型大麦萌发期抗旱特性,筛选萌发期抗旱性鉴定关键指标,建立萌发期抗旱性研究数学模型,为抗旱大麦品种培育提供理论指导。【方法】采用人工气候箱内发芽盒培养,用20%PEG8000溶液对吉33等12份中亚引进大麦品种进行萌发期抗旱试验,测定相应材料对照和胁迫下种子发芽势(GP)、发芽率(GR)、发芽指数(GI)、根数(RN)、根长(RL)、苗高(SH)和胚芽鞘长(CL)7个生长指标。以各指标相对值作为衡量萌发期抗旱性的依据,利用多元统计分析方法对不同大麦品种萌发期抗旱性进行综合评价。【结果】PEG胁迫下,参试材料各指标均较对照呈下降趋势,各指标彼此之间呈显著或极显著相关性,大麦萌发受到不同程度的抑制。利用隶属函数法综合评价发现不同大麦品种间表现出较大差异,吉33抗旱性最强,吉引2013-7-DM-088抗旱性最弱。依据主成分分析,将7个单项指标转换得到2个相互独立因子,它们代表了所测指标85.94%的信息。进行多元逐步回归线性分析建立大麦萌发期抗旱性评价数学模型,D=0.036+0.392GR+0.382SH+0.233GP(R2=0.992)。【结论】吉33抗旱性最强,发芽率、苗高和发芽势可作为快速评价大麦萌发期抗旱性的关键指标。 展开更多
关键词 大麦 萌发期 抗旱性 主成分分析 回归分析
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芽麦的研究进展 预览
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作者 徐颖 党长英 +1 位作者 沈汪洋 王展 《粮食与油脂》 北大核心 2019年第6期4-7,共4页
发芽会使小麦的品质发生一系列变化,根据国内外研究现状,综述了芽麦的产生原因及应用现状,探讨了小麦发芽后淀粉、蛋白质、γ-氨基丁酸等主要营养成分的变化,对面团特性的影响及其应用研究现状,以期为芽麦的合理利用提供基础理论数据。
关键词 小麦 发芽 品质 应用
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不同配比沙培基质对奶白菜发芽和生长的影响 预览
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作者 樊煜 李治勤 +2 位作者 晏清洪 王泽 左慧琴 《中国瓜菜》 CAS 北大核心 2019年第2期40-42,共3页
为寻求利于奶白菜生长的基质配比,以农业生产中常用的沙子、草炭、腐殖酸为基质,通过调查奶白菜的发芽势、发芽率、株高、开展度、产量等指标,研究不同配比基质对沙培奶白菜发芽和生长的影响。结果表明,各处理下的发芽情况较好,但6种配... 为寻求利于奶白菜生长的基质配比,以农业生产中常用的沙子、草炭、腐殖酸为基质,通过调查奶白菜的发芽势、发芽率、株高、开展度、产量等指标,研究不同配比基质对沙培奶白菜发芽和生长的影响。结果表明,各处理下的发芽情况较好,但6种配比基质对奶白菜的发芽势和发芽率并无显著影响,对奶白菜株高、开展度的影响显著,T2、T3的株高和开展度好于其他处理,株高最高为27.10cm,开展度最大为22.37cm;对奶白菜产量的影响显著,T2组的产量最高,为1.404kg·m^-2,其次是T3,产量为1.341kg·m^-2。生产上推荐使用T2:30%沙子+60%草炭+10%腐殖酸和T3:40%沙子+60%草炭这2种基质。 展开更多
关键词 奶白菜 沙培 发芽 生长
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冷害环境下遮阴处理对大果榛子萌芽生长的影响 预览
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作者 王德新 张晏 +3 位作者 段安安 逄宏扬 龙作义 李丽萍 《经济林研究》 北大核心 2019年第3期198-202,共5页
为给大果榛子的规模引种提供参考,以‘龙榛’品种为材料,就叶芽和花芽萌动期其在冷害和不同光照强度条件下的生长状态进行分析。结果表明:枝条直径生长量方面,遮阴处理的平均值为5.26mm,未遮阴处理的平均值为7.91mm,未遮阴处理的大果榛... 为给大果榛子的规模引种提供参考,以‘龙榛’品种为材料,就叶芽和花芽萌动期其在冷害和不同光照强度条件下的生长状态进行分析。结果表明:枝条直径生长量方面,遮阴处理的平均值为5.26mm,未遮阴处理的平均值为7.91mm,未遮阴处理的大果榛子枝条直径与遮阴处理的存在极显著差异;芽长宽比方面,遮阴处理的平均值为1.61,未遮阴处理的平均值为1.49,2个处理间差异不显著。在遮阴处理下,大果榛子的营养生长(茎粗和叶芽大小)和生殖生长(花芽的萌发)均受到抑制,全光照条件下生长正常。冷害(≥2℃)并未对大果榛子萌发的叶芽和花芽造成低温伤害,光照强度对大果榛子生长影响显著。 展开更多
关键词 大果榛子 萌芽 光强度 冷害 生长发育
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半枫荷不同播种时间育苗试验
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作者 徐水根 《林业建设》 2019年第2期51-53,共3页
采用不完全随机区组设计,分析雨水(Y处理)、惊蛰(J处理)、春分(C处理)三个不同节气播种育苗的种子发芽率、保存率、平均苗高和平均地径的试验结果,试验结果表明三种不同播种时间以J处理生长状况为好,综合分析认为:半枫荷实生播种育苗适... 采用不完全随机区组设计,分析雨水(Y处理)、惊蛰(J处理)、春分(C处理)三个不同节气播种育苗的种子发芽率、保存率、平均苗高和平均地径的试验结果,试验结果表明三种不同播种时间以J处理生长状况为好,综合分析认为:半枫荷实生播种育苗适宜节气为惊蛰前后较为适宜。 展开更多
关键词 半枫荷 种子育苗 不同时间 发芽 生长量
Study on the Ecotoxicity of Three Veterinary Antibiotics Against Alfalfa ( Medicago sativa L.) 预览
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作者 Jisen XU xiangnan YUE 《农业生物技术:英文版》 CAS 2019年第3期166-168,共3页
[Objectives]This study was conducted on the effects of veterinary antibiotics on pasture growth.[Methods]With alfalfa ( Medicago sativa L.) as an experimental material and oxytetracycline,chlortetracycline and enroflo... [Objectives]This study was conducted on the effects of veterinary antibiotics on pasture growth.[Methods]With alfalfa ( Medicago sativa L.) as an experimental material and oxytetracycline,chlortetracycline and enrofloxacin as experimental contaminants,the effects of the three veterinary antibiotics on the germination percentage,germination energy,germination index and vigor index of alfalfa seeds and on the plant height and root length of alfalfa seedlings were study at the concentration gradient of 50,100,150,200 and 250 mg/L,respectively.[Results]The oxytetracycline treatments had no significant effect on the germination percentage of alfalfa seeds,but promoted its germination index.The chlortetracycline treatment solutions and enrofloxacin treatment solutions promoted the germination percentage,germination energy and germination index of alfalfa.The three antibiotics significantly inhibited the vigor index of alfalfa seeds,and had significant inhibitory effects on root length and plant height.[Conclusions]These results provide an important basis for studying the ecotoxicity of veterinary antibiotics on pasture. 展开更多
关键词 VETERINARY ANTIBIOTICS ALFALFA Seed germination SEEDLING growth ECOTOXICITY
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苯磺隆胁迫下油菜萌发期相关性状的全基因组关联分析 预览
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作者 周清元 王倩 +8 位作者 叶桑 崔明圣 雷维 郜欢欢 赵愉风 徐新福 唐章林 李加纳 崔翠 《中国农业科学》 CAS CSCD 北大核心 2019年第3期399-413,共15页
【目的】研究苯磺隆残留对油菜种子萌发的影响,运用全基因组关联分析(genome-wide association analysis,GWAS)揭示苯磺隆胁迫下油菜萌发期相关性状的遗传因子和候选基因,探究油菜在苯磺隆逆境胁迫下的生理形态所反映的基因调控机制,为... 【目的】研究苯磺隆残留对油菜种子萌发的影响,运用全基因组关联分析(genome-wide association analysis,GWAS)揭示苯磺隆胁迫下油菜萌发期相关性状的遗传因子和候选基因,探究油菜在苯磺隆逆境胁迫下的生理形态所反映的基因调控机制,为耐苯磺隆油菜品种的研究提供参考。【方法】以241份甘蓝型油菜品种(系)为材料、25 mg·L-1苯磺隆溶液为处理液、蒸馏水为对照进行发芽试验。发芽7 d测定并计算相对发芽率、相对根长和相对鲜重。结合芸薹属60K SNP芯片分析群体基因型,通过STRUCTURE软件和TASSEL软件分别对该群体进行群体结构分析以及亲缘关系和LD衰减分析。为有效排除假关联的影响,采用一般线性模型(GLM)和混合线性模型(MLM)中的6种模型进行比较,确定每个性状GWAS分析的最优模型。同时,利用TASSEL软件在最优模型下对241份材料的3个性状分别进行全基因组关联分析,根据关联SNP位点的LD区间序列预测候选基因。【结果】241份品种(系)群体可分为P1(94份材料)和P2(147份材料)2个亚群,其中约56.28%的材料之间的亲缘关系值为0。全基因组关联分析(K+PCA模型)共检测到16个与性状显著关联的SNP位点,这些位点可解释9.42%—13.14%的表型变异率。通过分析显著SNP位点的LD区间与甘蓝型油菜对应的区间序列,筛选出25个候选基因可能与油菜耐苯磺隆有关,其中9个为细胞色素P450家族基因,5个参与谷胱甘肽合成或代谢过程,2个为多药耐药相关蛋白基因。同时发现与相对发芽率显著相关的基因ATGSTU19编码谷胱甘肽转移酶,参与毒素分解过程,在各种胁迫反应中起重要作用。在相对根长和相对鲜重共同鉴定到的候选基因BnaC02g27690D功能未知。【结论】共检测到16个SNP位点与耐苯磺隆性状显著关联,筛选出25个候选基因可能与油菜耐苯磺隆有关。 展开更多
关键词 油菜 萌发期 苯磺隆 全基因组关联分析
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3种青稞发芽过程中营养物质的变化 预览
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作者 王波 张文会 《食品研究与开发》 CAS 北大核心 2019年第6期26-30,35共6页
为探究发芽过程中青稞籽粒营养物质的变化机制,以藏青2000、隆子黑青稞和冬青18为原料,在相同条件下发芽,通过测定发芽过程中蛋白质、淀粉、脂肪、膳食纤维、γ-氨基丁酸、β-葡聚糖和总黄酮等含量的变化,研究发芽时间对青稞营养价值的... 为探究发芽过程中青稞籽粒营养物质的变化机制,以藏青2000、隆子黑青稞和冬青18为原料,在相同条件下发芽,通过测定发芽过程中蛋白质、淀粉、脂肪、膳食纤维、γ-氨基丁酸、β-葡聚糖和总黄酮等含量的变化,研究发芽时间对青稞营养价值的影响。结果表明:发芽过程中,蛋白质、淀粉、脂肪和β-葡聚糖在发芽前期均下降,但在后期变化平缓且有上升的趋势;而膳食纤维、总黄酮和γ-氨基丁酸含量有所提高,尤其发芽中期,γ-氨基丁酸增幅明显。在相同培养条件下,不同青稞品种间营养物质的含量没有较显著的差异,但是相比其他两种,发芽隆子黑青稞在富含γ-氨基丁酸、β-葡聚糖和总黄酮方面具有部分优势。通过发芽,能在一定程度上提高青稞籽粒的营养价值,但对其在利用β-葡聚糖相关功能特性方面有一定的限制。 展开更多
关键词 青稞 发芽 不同品种 营养成分 活性物质
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藏药多刺绿绒蒿种子萌发特性研究 预览
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作者 袁芳 宋凯杰 +1 位作者 蔡熙彤 兰小中 《广西植物》 CAS CSCD 北大核心 2019年第7期902-909,共8页
多刺绿绒蒿(Meconopsishorridula)为罂粟科绿绒蒿属一年生草本植物,是一种极具观赏价值和药用价值的高山植物,目前处于濒危状态,因此研究多刺绿绒蒿种子的萌发特性对其种子育苗及人工栽培具有重要意义。为了提高多刺绿绒蒿的种子发芽率... 多刺绿绒蒿(Meconopsishorridula)为罂粟科绿绒蒿属一年生草本植物,是一种极具观赏价值和药用价值的高山植物,目前处于濒危状态,因此研究多刺绿绒蒿种子的萌发特性对其种子育苗及人工栽培具有重要意义。为了提高多刺绿绒蒿的种子发芽率,该研究以多刺绿绒蒿的种子为材料,分析了不同消毒剂、浸种时间、温度和外源植物激素对种子萌发特性的影响。结果表明:(1)最适消毒方法为75%乙醇1min+3%H2O25min,最适浸种时间为24h,最适温度和光照条件为20℃/10℃(光照12h/黑暗12h),用无菌水浸种后的种子发芽率为49.67%。(2)GA3100~600mg·L^-1和NAA5~30mg·L^-1可以提高种子的发芽率、发芽势和发芽指数,缩短发芽启动时间和发芽持续时间,对种子的萌发有促进作用。(3)6-BA5mg·L^-1和10mg·L^-1对种子的萌发有一定的促进作用,但不显著,6-BA浓度≥15mg·L^-1则抑制种子的萌发。(4)用GA3500mg·L^-1浸种后的种子发芽指标最好,发芽率、发芽势和发芽指数分别为69.67%、33.00%、4.51,种子的发芽起始时间和发芽持续时间分别为10.67d、11.67d。 展开更多
关键词 多刺绿绒蒿 种子 萌发 消毒剂 浸种时间 温度 外源植物激素
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青稞籽粒富硒发芽条件优化及其抗氧化能力分析 预览
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作者 宋妍 李粟晋 +4 位作者 陶阳 韩永斌 朱筱玉 谢广杰 叶明儒 《食品工业科技》 CAS 北大核心 2019年第14期188-195,202共9页
采用响应面法研究发芽时间、培养液pH和亚硒酸钠浓度对青稞籽粒中有机硒含量的影响,得到最佳富硒发芽工艺条件。在此条件下,进一步对青稞籽粒发芽过程中抗氧化物质含量及抗氧化能力进行分析。结果表明:青稞籽粒最佳富硒发芽工艺为发芽时... 采用响应面法研究发芽时间、培养液pH和亚硒酸钠浓度对青稞籽粒中有机硒含量的影响,得到最佳富硒发芽工艺条件。在此条件下,进一步对青稞籽粒发芽过程中抗氧化物质含量及抗氧化能力进行分析。结果表明:青稞籽粒最佳富硒发芽工艺为发芽时间48.00h、pH6.00、亚硒酸钠浓度10.00mg/L,在此条件下,青稞籽粒有机硒含量为1.572mg/kgDW,为原料的17.46倍。富硒发芽可显著提高青稞籽粒中总酚、总黄酮醇、有机硒含量以及ABTS+自由基清除能力(p<0.05),尽管降低了β-葡聚糖含量及铁离子还原能力(FRAP),但与未富硒组相比,富硒可有效延缓β-葡聚糖降解。相关性分析表明,总酚含量与有机硒含量、ABTS+自由基清除能力,β-葡聚糖含量与铁离子还原能力(FRAP)以及有机硒含量与ABTS+自由基清除能力均呈极显著正相关(p<0.01),其他指标间呈负相关或不相关。本研究表明,富硒处理可增加发芽青稞籽粒抗氧化物质含量,有效延缓β-葡聚糖降解,增强其抗氧化能力。 展开更多
关键词 青稞籽粒 富硒 发芽 抗氧化物质 抗氧化能力
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60Co-γ辐射对谷稗幼苗生长及生理效应的影响 预览
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作者 李波 邬婷婷 马赫 《云南农业大学学报:自然科学版》 CSCD 北大核心 2019年第1期83-88,共6页
【目的】探究不同60Co-γ射线辐射对朝牧1号谷稗种子幼苗生长以及生理效应的影响。【方法】以朝牧1号谷稗种子为试材,采用0、50、100、150、200和250 Gy的60Co-γ射线辐射谷稗种子,对不同辐射剂量的谷稗种子进行幼苗培养,测定辐射对其... 【目的】探究不同60Co-γ射线辐射对朝牧1号谷稗种子幼苗生长以及生理效应的影响。【方法】以朝牧1号谷稗种子为试材,采用0、50、100、150、200和250 Gy的60Co-γ射线辐射谷稗种子,对不同辐射剂量的谷稗种子进行幼苗培养,测定辐射对其幼苗的生长和叶片生理生化的影响,并对各指标进行相关性和主成分分析。【结果】辐射抑制了谷稗幼苗的生长,250 Gy对其幼苗的生长抑制作用较强;随着辐射剂量的增加,可溶性糖、脯氨酸、可溶性蛋白和总叶绿素含量均呈先增后减的变化趋势,相对电导率和丙二醛含量呈先减后增的变化趋势,过氧化物酶(POD)、过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活性均呈先增后减的变化趋势。【结论】综合主成分的方差贡献率和主成分的原性状相关矩阵值计算出特征向量分析发现,50~200 Gy辐射剂量可用于谷稗的辐射育种。 展开更多
关键词 谷稗 60CO-Γ辐射 幼苗生长 生理指标 相关性分析 主成分分析
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Evaluating germinability of eight desert halophytes under long-term seed storage:Implications for conservation
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作者 Sanjay Gairola Hatem A. Shabana +2 位作者 Tamer Mahmoud Ali El-Keblawy Andrea Santo 《植物多样性:英文版》 CAS CSCD 2019年第4期229-236,共8页
Ex situ conservation in seed banks is a potential complementary conservation strategy for native plant species.It is well established that ex situ seed banking of native wild plants prolongs seed viability and thereby... Ex situ conservation in seed banks is a potential complementary conservation strategy for native plant species.It is well established that ex situ seed banking of native wild plants prolongs seed viability and thereby preserves genetic and species diversity for future use.We evaluated ex situ storage potential of eight halophytic species from deserts in the United Arab Emirates(UAE)by studying seed germination.Specifically,we examined the germinability of freshly collected seeds and seeds stored for three years in a seed bank.We also examined the effect of light conditions on fresh and stored seed germination.Fresh seeds of seven of the eight species tested had a higher germination rates under 12/12 h light/dark fluctuations than did those exposed to total darkness.Storage reduced light sensitivity in Halocnemum strobilaceum,Suaeda aegyptiaca,Salsola drummondii and Salsola imbricata,but increased the requirement for light in Arthrocnemum macrostachyum.In Anabasis setifera,storage decreased germination percentage when there was a 12-hour light/dark fluctuation,but increased germination rate when exposed to the dark treatment.Storage significantly reduced germination in both the light/dark and dark treatments in Suaeda vermiculata and S.aegyptiaca.Germination speed also responded differently to storage;whereas Timson's index significantly increased in A.macrostachyum and H.strobilaceum,it significantly decreased for S.drummondii,S.aegyptiaca and S.vermiculata.Germination of these species at a range of temperatures requires further testing;additionally,we strongly suggest that these laboratory findings be complemented by field studies. 展开更多
关键词 Ex situ CONSERVATION HALOPHYTES SEED bank collection SEED GERMINATION Salt tolerance SSBH
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