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Correlation of Commonly Used Anthropometric Parameters with FPG,FPG-CV and HbA1C 预览
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作者 Ya-Nan Zhao Rui-Hua Wang +2 位作者 Li-Juan Zhang Qiang Xu Yan-Fen Li 《TMR食疗与健康管理》 2019年第1期2-7,共6页
Objective:To investigate the relationship between body mass index(BMI),waist circumference(WC),hip circumference(HC),waist-hip ratio(WHR),waist height ratio(WHtR),fasting blood glucose(FPG),fasting blood glucose varia... Objective:To investigate the relationship between body mass index(BMI),waist circumference(WC),hip circumference(HC),waist-hip ratio(WHR),waist height ratio(WHtR),fasting blood glucose(FPG),fasting blood glucose variation(FPG-CV)and glycosylated hemoglobin(HbA1C)to provide a reference for predicting the risk and development trend of diabetes mellitus.Methods:From October 2016 to December 2017,111 subjects from the Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine were selected to measure their height and weight.BMI,WHR and WHtR were calculated and the concentrations of FPG and HbA1C were detected.Statistical analysis was carried out with SPSS21.0 software,and the data were processed by multiple linear regression analysis.Results:The concentration dependence of WHtR,FPG,FPG-CV and HbA1C were more closely related.There was a significant difference between WHtR and FPG,FPG-CV and HbA1C by multiple linear regression analysis(t=8.531,6.910 and 6.905,respectively,P<0.01),and the correlation coefficient was 0.633,0.552 and 0.552 respectively(P<0.01).Conclusion:There is a significant correlation between WHtR and FPG,FPG-CV and HbA1C.Therefore,measuring the height and waist circumference for the predictive potential of diabetes needs to be emphasized and intensified. 展开更多
关键词 FASTING blood GLUCOSE FASTING GLUCOSE variation GLYCOSYLATED HEMOGLOBIN Human parameters Correlation
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Update on biomarkers of glycemic control 预览
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作者 Maja Krhac Marijana Vucic Lovrencic 《世界糖尿病杂志:英文版(电子版)》 2019年第1期1-15,共15页
Attaining and maintaining good glycemic control is a cornerstone of diabetes care.The monitoring of glycemic control is currently based on the self-monitoring of blood glucose(SMBG)and laboratory testing for hemoglobi... Attaining and maintaining good glycemic control is a cornerstone of diabetes care.The monitoring of glycemic control is currently based on the self-monitoring of blood glucose(SMBG)and laboratory testing for hemoglobin A1c(HbA1c),which is a surrogate biochemical marker of the average glycemia level over the previous 2-3 mo period.Although hyperglycemia is a key biochemical feature of diabetes,both the level of and exposure to high glucose,as well as glycemic variability,contribute to the pathogenesis of diabetic complications and follow different patterns in type 1 and type 2 diabetes.HbA1c provides a valuable,standardized and evidence-based parameter that is relevant for clinical decision making,but several biological and analytical confounders limit its accuracy in reflecting true glycemia.It has become apparent in recent years that other glycated proteins such as fructosamine,glycated albumin,and the nutritional monosaccharide 1,5-anhydroglucitol,as well as integrated measures from direct glucose testing by an SMBG/continuous glucose monitoring system,may provide valuable complementary data,particularly in circumstances when HbA1c results may be unreliable or are insufficient to assess the risk of adverse outcomes.Long-term associations of these alternative biomarkers of glycemia with the risk of complications need to be investigated in order to provide clinically relevant cut-off values and to validate their utility in diverse populations of diabetes patients. 展开更多
关键词 Diabetes mellitus HEMOGLOBIN A1C FRUCTOSAMINE Glycated ALBUMIN 1 5-ANHYDROGLUCITOL Plasma GLUCOSE GLUCOSE variability Diabetic complications
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Mechanism Research of Reducing Obesity-Induced Insulin Resistance in the White Adipose Tissue by Knockdown of Neuropeptide Y Expression in the Dorsomedial Hypothalamus
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作者 ZHAI Qiuran QIN Qian +4 位作者 CHEN Peng CUI Zhihui WANG Jiao ZHANG Jianxiang WANG Shoujun 《武汉大学学报:自然科学英文版》 CAS CSCD 2019年第1期45-56,共12页
This study investigated the specific mechanism of knockdown of neuropeptide Y(NPY)in reducing obesity-induced insulin resistance in the white adipose tissue.Adeno-associated virus(AAV)-mediated RNAi was utilized to do... This study investigated the specific mechanism of knockdown of neuropeptide Y(NPY)in reducing obesity-induced insulin resistance in the white adipose tissue.Adeno-associated virus(AAV)-mediated RNAi was utilized to downregulate NPY expression in rats fed either regular chow or high fat diet.By investigating the differences in rat body weight and food intake,we assessed the effect of knockdown of NPY expression on insulin sensitivity andβ-cell proliferation.Glucose consumption and 2-[3 H]DG uptake in 3 T3-L1 adipocytes were assessed to determine the molecular mechanisms.The results showed that knockdown of NPY expression in the dorsomedial hypothalamus(DMH)reduced obesity-induced insulin resistance,increased glucose consumption,and decreased 2-[3 H]DG uptake in 3 T3-L1 adipocytes via the PI3 K/Akt/GSK-3βsignaling pathways and the NPY Y5 receptor. 展开更多
关键词 KNOCKDOWN of NEUROPEPTIDE Y(NPY) insulin resistance GLUCOSE consumption GLUCOSE INTAKE PI3K/Akt/GSK-3βsignaling PATHWAYS
碳源对O/A-F/F模式积累内源聚合物及反硝化的影响
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作者 崔有为 金常林 +1 位作者 王好韩 李晶 《环境科学》 EI CAS CSCD 北大核心 2019年第1期336-342,共7页
好氧/缺氧-盛宴/饥饿(O/A-F/F)选择模式能够在好氧段实现活性污泥积累内源聚合物的同时在缺氧段原位利用内源聚合物驱动反硝化.为了深入探究不同的碳源类型对O/A-F/F模式下内源聚合物积累和内源反硝化的影响,实验以乙酸和葡萄糖为主要... 好氧/缺氧-盛宴/饥饿(O/A-F/F)选择模式能够在好氧段实现活性污泥积累内源聚合物的同时在缺氧段原位利用内源聚合物驱动反硝化.为了深入探究不同的碳源类型对O/A-F/F模式下内源聚合物积累和内源反硝化的影响,实验以乙酸和葡萄糖为主要碳源探究内源聚合物积累和内源反硝化特性以及富集的活性污泥菌群的结构和功能.结果表明,在O/A-F/F选择模式下,当进水化学需氧量(COD)为500 mg·L^-1左右时,以乙酸为主要碳源系统(Ac-SBR)和以葡萄糖为主要碳源的系统(Gc-SBR)均能实现40 mg·L^-1的硝酸盐氮的内源去除,且各系统均实现了部分短程反硝化.但Ac-SBR实现了更高的亚硝酸盐的积累.乙酸有利于内源聚羟基脂肪酸酯(PHA)积累并驱动内源反硝化过程,PHA产率为0.52,平均反硝化速率(DNR)为9.65 mg·(L·h)^-1.Gc-SBR系统能够实现PHA和糖原(Gly)的同时积累,但Gly产率高于PHA产率,分别为0.36和0.17,DNR为4.35 mg·(L·h)^-1.Gly是实现内源反硝化过程的主要驱动力,反硝化脱氮贡献率占总量的77%.16S rRNA高通量测序表明Proteobacteria门中的β-Proteobacteria在Ac-SBR中为优势菌纲,菌群丰度为40.56%,而在Gc-SBR中菌群丰度为18.05%.α-Proteobacteria可能在Gc-SBR中贡献了微生物的糖原积累.β-Proteobacteria、Unclassified Bacteroidetes和Lgnavibacteria在Ac-SBR中贡献了内源PHA积累. 展开更多
关键词 好氧/缺氧-盛宴/饥饿模式(O/A-F/F) 乙酸 葡萄糖 内源聚合物积累 内源反硝化
Aspirin inhibits the proliferation of hepatoma cells through controlling GLUT 1-mediated glucose metabolism
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作者 Yun-xia Liu Jin-yan Feng +8 位作者 Ming-ming Sun Bo-wen Liu Guang Yang Ya-nan Bu Man Zhao Tian-jiao Wang Wei-ying Zhang Hong-feng Yuan Xiao-dong Zhang 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第1期122-132,共11页
Aspirin can efficiently inhibit liver cancer growth,but the mechanism is poorly,understood.In this study,we-report that aspirin modulates glucose uptake through downregulating glucose transporter 1 (GLUT1),leading to ... Aspirin can efficiently inhibit liver cancer growth,but the mechanism is poorly,understood.In this study,we-report that aspirin modulates glucose uptake through downregulating glucose transporter 1 (GLUT1),leading to the inhibition of hepatoma cell proliferation.Our data showed that aspirin significantly decreased the levels of reactive oxygen species (ROS)and glucose consumption in hepatoma cells.Interestingly,we identified that GLUT1 and HIF1α could be decreased by aspirin.Mechanically,we demonstrated that the -1008/-780 region was the regulatory element of transcriptional factor NF-κB in GLUT1 promoter by luciferase report gene assays.PDTC,an inhibitor of NF-KB,could suppress the expression of GLUT1 in HepG2 and H7402 cells,: followed by affecting the levels of ROS and glucose consumption.CoCl2-activated HIF1α expression could slightly rescue the GLUT1 expression inhibited by aspirin or PDTC,suggesting that aspirin depressed GLUT1 through targeting NF-κB or NF-κB/HIFla signaling.Moreover,we found that GLUT1 was highly expressed in clinical HCC tissues relating to their paired adjacent normal tissues.Importantly,we observed that high level of GLUT1 was significantly correlated with the poor relapse-free survival of HCC patients by analysis of public data.Functionally,overexpression of GLUT1 blocked the PDTC-induced or aspirin-induced inhibition of glucose metabolism in HepG2 cells.Conversely,aspirin failed to work when GLUT1 was stably knocked down in the cells. Administration of aspirin could depress the growth of hepatoma cells through controlling GLUT1 in vitro and in vivo.Thus,our finding provides new insights into the mechanism by which aspirin depresses liver cancer. 展开更多
关键词 ASPIRIN GLUCOSE UPTAKE GLUT1 NF-κB liver cancer
Novel Cancer Treatment Using Targeted Delivery of Short-Lived Radiologically Activated Nanoparticles 预览
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作者 P.L.Andrade J.B.Leao 《健康科学:英文版》 2019年第1期43-47,共5页
The goal of our preliminary study is to show that a minimal of overall body toxicity while maximizing the cancer tumor treatment may be achieved in a novel approach where minute amounts of coated nanoparticles may be ... The goal of our preliminary study is to show that a minimal of overall body toxicity while maximizing the cancer tumor treatment may be achieved in a novel approach where minute amounts of coated nanoparticles may be radiologically activated prior to treatment and“coerce”tumor cells into readily absorb these nanoparticles.This targeted intracytoplasmic delivery of short-lived radiologically activated nanoparticles could provide less“whole-body”radiation dose while delivering a short lived potent tumor localized dose,along with their low toxicity may prove to be another tool in the treatment of diverse cancers. 展开更多
关键词 NANOPARTICLES NEUTRON activation irradiation cancer METABOLISM WARBURG effect GLUTAMINE GLUCOSE
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不同盐度条件下葡萄糖对余氯等水质的影响 预览
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作者 秦海鹏 许威威 +6 位作者 王博 廖栩峥 胡世康 刘雪婷 郑义华 苏玉芹 孙成波 《安徽农业科学》 CAS 2019年第7期94-97,共4页
[目的]研究葡萄糖在不同盐度条件下对余氯的去除效果,水体中微生物的生长衰亡对水体中三氮的影响,以及余氯对水体pH的影响。[方法]在4个盐度梯度(0、10、20、30)条件下设置相同浓度的葡萄糖,研究葡萄糖对水中总余氯的去除效果,同时计算... [目的]研究葡萄糖在不同盐度条件下对余氯的去除效果,水体中微生物的生长衰亡对水体中三氮的影响,以及余氯对水体pH的影响。[方法]在4个盐度梯度(0、10、20、30)条件下设置相同浓度的葡萄糖,研究葡萄糖对水中总余氯的去除效果,同时计算各个盐度7d内的水体水质指标。[结果]在30h时盐度为30的水体中总余氯浓度最先降至0,与其他3组显著差异(P<0.05)。盐度为0时,弧菌无法生长。4个不同盐度梯度下的总菌数目24h后均不同程度的增长,此后细菌数量开始减少衰亡,4组之间差异显著(P<0.05)。在7个时间点内盐度为0的水体pH比其他3个盐度梯度的pH均高,盐度0与另外3组差异显著(P<0.05)。在7个时间内4个盐度梯度的氨氮先缓慢升高然后上升速度逐渐变快,最后快速下降。在7个时间内硝酸盐变化趋向于平缓,波动不大,4组之间差异不显著(P>0.05)。4个盐度梯度的亚硝酸盐含量在0~72h出现微小波动,但72h后开始略有升高且在升高后保持稳定。[结论]在消毒水体中加入葡萄糖作为中和余氯的碳源,不同盐度对葡萄糖中和余氯的效率有影响,盐度越高,中和效率越高,且水体中的三氮随着水体中细菌数量的增长衰亡呈现规律变化。 展开更多
关键词 盐度 葡萄糖 去除余氯 三氮 菌群
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脱脂牛乳体系中乳蛋白-葡萄糖美拉德反应程度及产物功能性质研究 预览
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作者 牟全生 杨敏 +5 位作者 王裕成 包鹏杰 雷敬敬 陈浩 王海霞 杨继涛 《食品工业科技》 CAS 北大核心 2019年第12期1-6,共6页
以脱脂牛乳为研究对象,以葡萄糖为还原糖,对乳蛋白进行美拉德反应修饰,研究葡萄糖添加量为0~9 g/100 mL脱脂乳时反应体系的糖基化程度、pH、中间产物含量、褐变程度、粒径,以及产物乳化性、发泡性和吸油性。结果显示,脱脂乳体系糖基化... 以脱脂牛乳为研究对象,以葡萄糖为还原糖,对乳蛋白进行美拉德反应修饰,研究葡萄糖添加量为0~9 g/100 mL脱脂乳时反应体系的糖基化程度、pH、中间产物含量、褐变程度、粒径,以及产物乳化性、发泡性和吸油性。结果显示,脱脂乳体系糖基化程度在葡萄糖添加量为3 g/100 mL时达到最大值,之后迅速降低;中间产物和褐变程度均在该添加量下达到最低值,之后迅速上升。反应体系pH并未随葡萄糖添加量的增加而显著变化。糖基化反应后,酪蛋白胶束粒径增加。糖基化修饰改善了脱脂乳体系的乳化性,当葡萄糖添加量为3 g/100 mL时,乳化活性和乳化稳定性均达到最大值。糖基化修饰后,脱脂乳体系的发泡性并未显著提高,但该体系10和30 min泡沫稳定显著增加(p<0.05)。糖基化修饰后乳蛋白的吸油性降低。研究结论可为脱脂乳体系中乳蛋白美拉德反应产物的应用提供基础数据。 展开更多
关键词 脱脂乳 糖基化 美拉德反应 葡萄糖
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高海藻糖和葡萄糖对豌豆蚜种群生命参数的影响
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作者 骆富强 王广 +4 位作者 白勇章 李岩 张银芳 李小伟 刘长仲 《中国植保导刊》 北大核心 2019年第5期5-10,共6页
为了明确高海藻糖和葡萄糖对豌豆蚜种群生命参数的影响,将两种色型豌豆蚜的3d若蚜,饥饿12h后,连续人工饲喂4d高浓度(50mg/mL和100mg/mL)海藻糖和葡萄糖后,转接到离体叶片中饲养,观察高海藻糖和葡萄糖对豌豆蚜生长发育的影响。结果表明,... 为了明确高海藻糖和葡萄糖对豌豆蚜种群生命参数的影响,将两种色型豌豆蚜的3d若蚜,饥饿12h后,连续人工饲喂4d高浓度(50mg/mL和100mg/mL)海藻糖和葡萄糖后,转接到离体叶片中饲养,观察高海藻糖和葡萄糖对豌豆蚜生长发育的影响。结果表明,高浓度葡萄糖和海藻糖(100mg/mL)会显著降低绿色型豌豆蚜种群净增殖率(R0),2种糖浓度处理之间无显著差异(P>0.05)。红色型豌豆蚜在2种糖浓度为50mg/mL的处理下R0无差异。2种浓度葡萄糖喂食下,均能降低绿色型豌豆蚜种群内禀增长率(rm)和周限增长率(λ);100mg/mL葡萄糖和海藻糖处理显著提高了2种色型豌豆蚜体内葡萄糖含量;100mg/mL海藻糖处理提高了绿色型豌豆蚜体内海藻糖含量。100mg/mL葡萄糖处理显著降低了2种色型豌豆蚜体内海藻糖含量。其结果表明,高海藻糖和葡萄糖的处理使豌豆蚜体内海藻糖和葡萄糖含量有显著变化,R0和rm显著降低。说明高糖对豌豆蚜的种群数量有降低作用。在红绿两种色型比较中,红色型豌豆蚜比绿色型豌豆蚜更加耐高糖。 展开更多
关键词 豌豆蚜 海藻糖 葡萄糖 生命表参数
Study on Accumulation and Dynamic Changes of Three Carbohydrates in Jerusalem Artichoke in Natural Habitats 预览
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作者 Lihui WANG Xuemei SUN +1 位作者 Long TAN Yi LI 《农业生物技术:英文版》 CAS 2019年第3期57-63,共7页
Two varieties of Jerusalem artichoke (early maturing variety Qingyu No.1 and middle maturing variety Qingyu No.2),which were bred independently by Research and Development Center of Jerusalem Artichoke,Qinghai Academy... Two varieties of Jerusalem artichoke (early maturing variety Qingyu No.1 and middle maturing variety Qingyu No.2),which were bred independently by Research and Development Center of Jerusalem Artichoke,Qinghai Academy of Agriculture and Forestry,were used as materials,and the content and dynamic changes of three kinds of carbohydrates (sucrose,glucose and fructose) in various organs of Jerusalem artichoke in different periods in two natural habitats (water plain and low hill dry land) were studied.The results showed that the content of the three kinds of carbohydrates in Qingyu No.1 and Qingyu No.2 in the water plain and low hill dry land was relatively high in late June and late August.The total content of the three carbohydrates in Qingyu No.1 peaked earlier than that of Qingyu No.2.The total content of the three carbohydrates in the water plain peaked earlier than that of the low hill dry land.In the whole growth period,the total content of the three carbohydrates in the stems and roots of Jerusalem artichoke was higher than that of the leaves.In the leaves,fructose accumulation was relatively obvious in the whole growth period,and the content was high,followed by glucose.In the stems and roots,fructose content was high,followed by sucrose.In the tubers,glucose accumulation was obvious,and sucrose content was lower than glucose and fructose content.When the tubers were harvested,fructose content was high. 展开更多
关键词 JERUSALEM ARTICHOKE Carbohydrates SUCROSE GLUCOSE FRUCTOSE Dynamic CHANGES
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CuO/EMT沸石复合材料的制备及其在无酶葡萄糖传感器中的应用 预览
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作者 黄文峰 陈晋阳 +1 位作者 王英迪 邹米华 《上海大学学报:自然科学版》 CAS CSCD 北大核心 2019年第2期301-308,共8页
在比较温和简单的条件下合成具有高比表面、开放孔结构以及颗粒尺寸小的纳米EMT沸石,再通过浸渍焙烧法获得纳米CuO颗粒并负载于EMT沸石.将制得的材料应用于无酶葡萄糖传感器,结果表明该无酶传感器对葡萄糖的检测具有响应快、线性范围宽... 在比较温和简单的条件下合成具有高比表面、开放孔结构以及颗粒尺寸小的纳米EMT沸石,再通过浸渍焙烧法获得纳米CuO颗粒并负载于EMT沸石.将制得的材料应用于无酶葡萄糖传感器,结果表明该无酶传感器对葡萄糖的检测具有响应快、线性范围宽、检出限低等特点. 展开更多
关键词 EMT沸石 CUO 葡萄糖 无酶检测 传感器
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不同培养基与饲养细胞组合对多房棘球绦虫体外培养模型的影响 预览
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作者 韩振阳 邓子 +1 位作者 王泽宇 张示杰 《中国组织工程研究》 CAS 北大核心 2019年第19期3080-3085,共6页
背景:多房棘球绦虫原头节的体外成囊培养是提取与研究其生发层干细胞的实验基础。D-MEM与RPMI-1640是最适宜用于泡球蚴体外培养的商业培养基,HeLa细胞与大鼠肝癌细胞(ATCC No. CRL-1600)也是常用饲养细胞。目的:构建多房棘球绦虫体外培... 背景:多房棘球绦虫原头节的体外成囊培养是提取与研究其生发层干细胞的实验基础。D-MEM与RPMI-1640是最适宜用于泡球蚴体外培养的商业培养基,HeLa细胞与大鼠肝癌细胞(ATCC No. CRL-1600)也是常用饲养细胞。目的:构建多房棘球绦虫体外培养模型,观察泡球蚴在不同饲养细胞[HeLa细胞与大鼠肝癌细胞(ATCC No.CRL-1600)]及含有体积分数10%胎牛血清的不同培养基[D-MEM(高/低糖型)与RPMI-1640]下生长情况。方法:预先培养HeLa细胞及大鼠肝癌细胞(ATCC No.CRL-1600),从新疆石河子大学第一附属院实验动物中心提供的感染多房棘球绦虫6个月以上的种鼠体内提取原头节后分别与不同饲养细胞及培养基体外共同培养。实验经石河子大学医学院第一附属医院医学伦理委员会批准,批准号:2015-018-01,批准时间:2017-04-07。实验根据饲养细胞与培养基组合分为6组,每组加入1×10~6饲养细胞与50mL含体积分数10%胎牛血清的培养基及5000个原头节,Ⅰ组:HeLa细胞与1640培养基培养;Ⅱ组:HeLa细胞与D-MEM(高糖型)培养基培养;Ⅲ组:大鼠肝癌细胞与1640培养基培养;Ⅳ组:大鼠肝癌细胞与D-MEM(高糖型)培养基培养;Ⅴ组:HeLa细胞与D-MEM(低糖型)培养基培养;Ⅵ组:大鼠肝癌细胞与D-MEM(低糖型)培养基培养,观察泡球蚴原头节的生存、生长和发育,每隔7d计算原头蚴成囊率,使用目镜测微尺测量囊泡平均直径大小。结果与结论:①原头蚴在6种组合中均可长期存活,大部分都形成囊泡,囊泡直径分布在1-6 mm;②培养第56天原头蚴成囊率和囊泡平均直径均为大鼠肝癌细胞与D-MEM(高糖型)培养基组效果最佳(P <0.05),成囊率(72.08±1.79)%,囊泡平均直径(3.379±0.199) mm;③囊泡囊液蛋白定量检测结果显示6组囊液蛋白含量均低于体内囊泡,Ⅳ组蛋白含量高于其他5组(P <0.05);④结果证实:同条件下D-MEM(高糖型)培养基囊泡大小与数量优于其他2种培养基。� 展开更多
关键词 泡球蚴 体外培养 培养基 饲养细胞 囊泡 葡萄糖 囊液 直径 成囊率 蛋白定量
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Hyodeoxycholic acid protects the neurovascular unit against oxygen-glucose deprivation and reoxygenation-induced injury in vitro 预览
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作者 Chang-Xiang Li Xue-Qian Wang +3 位作者 Fa-Feng Cheng Xin Yan Juan Luo Qing-Guo Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1941-1949,共9页
Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin an... Calculus bovis is commonly used for the treatment of stroke in traditional Chinese medicine. Hyodeoxycholic acid(HDCA) is a bioactive compound extracted from calculus bovis. When combined with cholic acid, baicalin and jas-minoidin, HDCA prevents hypoxia-reoxygenation-induced brain injury by suppressing endoplasmic reticulum stress-mediated apoptotic signaling. However, the effects of HDCA in ischemic stroke injury have not yet been studied. Neurovascular unit(NVU) dysfunction occurs in ischemic stroke. Therefore, in this study, we investigated the effects of HDCA on the NVU under ischemic conditions in vitro. We co-cultured primary brain microvascular endothelial cells, neurons and astrocytes using a transwell chamber co-culture system. The NVU was pre-treated with 10.16 or 2.54 μg/mL HDCA for 24 hours before exposure to oxygen-glucose deprivation for 1 hour. The cell counting kit-8 assay was used to detect cell activity. Flow cytometry and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling were used to assess apoptosis. Enzyme-linked immunosorbent assay was used to measure the expression levels of inflammatory cytokines, including interleukin-1β, interleukin-6 and tumor necrosis factor-α, and neurotrophic factors, including brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor. Oxidative stress-related factors, such as superoxide dismutase, nitric oxide, malondialdehyde and γ-glutamyltransferase, were measured using kits. Pretreatment with HDCA significantly decreased blood-brain barrier permeability and neuronal apoptosis, significantly increased transendothelial electrical resistance and γ-glutamyltransferase activity, attenuated oxidative stress damage and the release of inflammatory cytokines, and increased brain-derived neurotrophic factor and glial cell line-derived neurotrophic factor expression. Our findings suggest that HDCA maintains NVU morphological integrity and function by modulating inflammation, oxidation stress, apoptosis, and the expression o 展开更多
关键词 hyodeoxycholic acid oxygen glucose deprivation and REOXYGENATION blood-brain barrier permeability anti-oxidative anti-inflammatory ANTI-APOPTOTIC BRAIN-DERIVED NEUROTROPHIC FACTOR glial cell line-derived NEUROTROPHIC FACTOR ischemic stroke in vitro NEUROVASCULAR unit
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在明串珠菌中构建葡萄糖到甘露醇的转化体系 预览
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作者 金红星 王星 彭钰玮 《食品与发酵工业》 CAS CSCD 北大核心 2019年第12期96-100,108共6页
为了进一步提高甘露醇产量,在明串珠菌构建了葡萄糖到甘露醇的转化体系。通过2次同源重组,将mt1d-m1p表达盒串联体定点插入到染色体上。以90g/L蔗糖为底物时,野生型菌株的甘露醇产量为31.48g/L,Δaldh::(mt1d-m1p)为42.63g/L,Δaldh::(m... 为了进一步提高甘露醇产量,在明串珠菌构建了葡萄糖到甘露醇的转化体系。通过2次同源重组,将mt1d-m1p表达盒串联体定点插入到染色体上。以90g/L蔗糖为底物时,野生型菌株的甘露醇产量为31.48g/L,Δaldh::(mt1d-m1p)为42.63g/L,Δaldh::(mt1d-m1p)Δdts::amy为43.47g/L,Δaldh::(mt1d-m1p)Δdts::(mt1d-m1p)为45.74g/L,Δdts1ΔD-ldhΔpat::mdhΔstpk::mdhΔfk::mdhΔaldh::(mt1d-m1p)为47.26g/L。增加甘露醇的合成途径是增产的手段之一。 展开更多
关键词 甘露醇 明串珠菌 葡萄糖 甘露醇-1-磷酸脱氢酶 甘露醇-1-磷酸酶
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Exploration of the shared pathophysiological mechanisms of gestational diabetes and large for gestational age offspring 预览
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作者 Sofia Nahavandi Sarah Price +1 位作者 Priya Sumithran Elif Ilhan Ekinci 《世界糖尿病杂志:英文版(电子版)》 2019年第6期333-340,共8页
Gestational diabetes mellitus (GDM) and large for gestational age (LGA) offspring are two common pregnancy complications. Connections also exist between the two conditions, including mutual maternal risk factors for t... Gestational diabetes mellitus (GDM) and large for gestational age (LGA) offspring are two common pregnancy complications. Connections also exist between the two conditions, including mutual maternal risk factors for the conditions and an increased prevalence of LGA offspring amongst pregnancies affected by GDM. Thus, it is important to elucidate potential shared underlying mechanisms of both LGA and GDM. One potential mechanistic link relates to macronutrient metabolism. Indeed, derangement of carbohydrate and lipid metabolism is present in GDM, and maternal biomarkers of glucose and lipid control are associated with LGA neonates in such pregnancies. The aim of this paper is therefore to reflect on the existing nutritional guidelines for GDM in light of our understanding of the pathophysiological mechanisms of GDM and LGA offspring. Lifestyle modification is first line treatment for GDM, and while there is some promise that nutritional interventions may favourably impact outcomes, there is a lack of definitive evidence that changing the macronutrient composition of the diet reduces the incidence of either GDM or LGA offspring. The quality of the available evidence is a major issue, and rigorous trials are needed to inform evidence-based treatment guidelines. 展开更多
关键词 GESTATIONAL diabetes MELLITUS Large for GESTATIONAL age Metabolism Biomarkers GLUCOSE LIPIDS
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葡萄糖在咪唑类离子液体中的溶解研究 预览
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作者 郑勇 郑永军 +1 位作者 王振 张艳维 《山东化工》 CAS 2019年第9期35-36,共2页
不同温度下,系统测定了葡萄糖在离子液体1-丁基-3-甲基咪唑四氟硼酸盐和1-丁基-3-甲基咪唑六氟磷酸盐中的溶解度。根据实验结果和理论公式,计算得到了溶解反应的主要热力学函数值。研究表明,1-丁基-3-甲基咪唑四氟硼酸盐对葡萄糖的溶解... 不同温度下,系统测定了葡萄糖在离子液体1-丁基-3-甲基咪唑四氟硼酸盐和1-丁基-3-甲基咪唑六氟磷酸盐中的溶解度。根据实验结果和理论公式,计算得到了溶解反应的主要热力学函数值。研究表明,1-丁基-3-甲基咪唑四氟硼酸盐对葡萄糖的溶解能力更强。葡萄糖在离子液体中的溶解是典型的吸热过程,受温度和溶解焓影响显著。 展开更多
关键词 葡萄糖 离子液体 溶解 热力学
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葡萄糖的添加对富锂锰基正极材料Li1.2Ni0.2Mn0.6O2的影响 预览
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作者 王雷 向延鸿 +5 位作者 廖启军 田林 吴贤文 吴显明 熊利芝 何则强 《吉首大学学报:自然科学版》 CAS 2019年第3期47-52,共6页
采用溶胶凝胶法制备合成富锂锰基正极材料Li1.2Ni0.2Mn0.6O2,在前期配制金属离子溶液时,通过添加不同量的葡萄糖(葡萄糖添加量分别为试剂总质量的0,6%,12%,36%,48%)来分析其对Li1.2Ni0.2Mn0.6O2的结构、形貌、电化学性能以及倍率性能的... 采用溶胶凝胶法制备合成富锂锰基正极材料Li1.2Ni0.2Mn0.6O2,在前期配制金属离子溶液时,通过添加不同量的葡萄糖(葡萄糖添加量分别为试剂总质量的0,6%,12%,36%,48%)来分析其对Li1.2Ni0.2Mn0.6O2的结构、形貌、电化学性能以及倍率性能的影响.恒流充放电测试结果显示,少量葡萄糖(6%,12%)加入,可以明显提高材料首次放电比容量.0.05C首次放电比容量由未加入葡萄糖材料的174 mAh/g提升至添加12%葡萄糖材料的265.9 mAh/g.倍率性能测试结果显示,葡萄糖的加入可以明显提高材料倍率性能.其中葡萄糖添加量为48%的材料倍率性能最好,首次放电比容量达到141 mAh/g,经过0.05C,0.1C,0.2C,0.5C,1C循环测试后再进行0.1C循环测试30次,放电比容量为110 mAh/g,容量保持率为78%. 展开更多
关键词 Li1.2Ni0.2Mn0.6O2 葡萄糖 电化学性能 倍率性能
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采用高内相乳液模板法制备葡萄糖基/麦芽糖基大孔材料及其形貌表征 预览
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作者 皮茂 张守村 +1 位作者 魏杰 李伟达 《材料导报》 EI CAS CSCD 北大核心 2019年第16期2804-2807,共4页
首先,在氢氧化钾的催化作用下,分别将葡萄糖、麦芽糖与甲基丙烯酸缩水甘油酯反应,得到甲基丙烯酰化的葡萄糖(G-GM)和麦芽糖(M-GM)两种水溶性单体。采用傅里叶变换红外光谱(FT-IR)仪、核磁共振波谱(1HNMR)仪确定其结构。然后以G-GM/M-GM... 首先,在氢氧化钾的催化作用下,分别将葡萄糖、麦芽糖与甲基丙烯酸缩水甘油酯反应,得到甲基丙烯酰化的葡萄糖(G-GM)和麦芽糖(M-GM)两种水溶性单体。采用傅里叶变换红外光谱(FT-IR)仪、核磁共振波谱(1HNMR)仪确定其结构。然后以G-GM/M-GM的水溶液为连续相,二氧化碳为分散相,二丙烯酸聚乙二醇酯(M W:1 000)为交联剂,制备水包二氧化碳型高内相乳液(内相体积分数大于74.05%),研究了表面活性剂的用量对乳液稳定性的影响。最后以过硫酸钾/四甲基乙二胺为引发剂,引发连续相中的单体聚合,得到大孔材料。实验结果显示,所得高内相乳液具有较好的稳定性,最高能稳定存在48 h以上。扫描电镜(SEM)分析表明,所得大孔材料的孔径分布与所用表面活性剂的浓度存在关系,其平均孔径分布在10~25μm之间,且存在大量的开孔结构,该材料有望在组织工程领域得到应用。 展开更多
关键词 高内相乳液 大孔材料 麦芽糖 葡萄糖 二氧化碳
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厌氧序批操作反应器中糖原储存及代谢模式研究
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作者 薛旭东 邓彦 +2 位作者 张振文 郑郁 曹筱晗 《环境科学与技术》 CAS CSCD 北大核心 2019年第1期108-111,共4页
文章研究了在以葡萄糖为基质的厌氧序批操作反应器(ASBR)中糖原的储存及代谢模式,结果表明:当进水结束时,基质水解的量占36.22%,基质储存的量占50.48%。胞内糖原的代谢受到糖原总量和胞外挥发性脂肪酸(VFA)含量的影响,糖原总量和代谢速... 文章研究了在以葡萄糖为基质的厌氧序批操作反应器(ASBR)中糖原的储存及代谢模式,结果表明:当进水结束时,基质水解的量占36.22%,基质储存的量占50.48%。胞内糖原的代谢受到糖原总量和胞外挥发性脂肪酸(VFA)含量的影响,糖原总量和代谢速率成正比,最高可达26.36 mg/(g VSS·h);胞外VFA含量和代谢速率成反比。'快速吸收储存、缓慢利用'是微生物利用糖原的过程中的主要特点,糖原的代谢模式为'葡萄糖-胞内糖原储存-VFA'。 展开更多
关键词 糖原 葡萄糖 ASBR 储存 代谢速率
工艺处理对金花茶花营养成分的影响
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作者 罗昭润 刘彰铭 +2 位作者 田雪琴 赵鸿杰 玄祖迎 《林业科技通讯》 2019年第2期49-51,共3页
通过测定活性成分、矿质元素、微量元素、氨基酸等营养成分含量,研究了制作工艺对金花茶花营养成分的影响。结果表明:制作工艺处理金花茶花活性成分茶多酚、茶多糖、黄酮和维生素E、维生素C含量分别升高249.28%、1 129.29%、390.16%和18... 通过测定活性成分、矿质元素、微量元素、氨基酸等营养成分含量,研究了制作工艺对金花茶花营养成分的影响。结果表明:制作工艺处理金花茶花活性成分茶多酚、茶多糖、黄酮和维生素E、维生素C含量分别升高249.28%、1 129.29%、390.16%和181.09%、47.02%,茶皂苷、咖啡碱含量分别下降4 530.36%和101.85%;矿质元素中K、Na、Ca、Mg、P含量分别升高719.06%、112.04%、592.57%、796.86%和872.27%;微量元素中Fe、Mn、Zn、Cu、Se含量分别升高248.74%、227.55%、350.30%、774.25%和477.78%;氨基酸总量增加972.72%。该工艺提高金花茶营养成分,可以用于制作金花茶花茶。 展开更多
关键词 金花茶 CAMELLIA nitidissima 葡萄糖 真空干燥 制作工艺 营养成分
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