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N-methyl-D-aspartate receptor subunit 1 regulates neurogenesis in the hippocampal dentate gyrus of schizophrenia-like mice 预览
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作者 Juan Ding Chun Zhang +4 位作者 Yi-Wei Zhang Quan-Rui Ma Yin-Ming Liu Tao Sun Juan Liu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2112-2117,共6页
N-methyl-D-aspartate receptor hypofunction is the basis of pathophysiology in schizophrenia. Blocking the N-methyl-D-aspartate receptor impairs learning and memory abilities and induces pathological changes in the bra... N-methyl-D-aspartate receptor hypofunction is the basis of pathophysiology in schizophrenia. Blocking the N-methyl-D-aspartate receptor impairs learning and memory abilities and induces pathological changes in the brain. Previous studies have paid little attention to the role of the N-methyl-D-aspartate receptor subunit 1 (NR1) in neurogenesis in the hippocampus of schizophrenia. A mouse model of schizophrenia was established by intraperitoneal injection of 0.6 mg/kg MK-801, once a day, for 14 days. In N-methyl-D-aspartate-treated mice, N-methyl-D-aspartate was administered by intracerebroventricular injection in schizophrenia mice on day 15. The number of NR1-, Ki67- or BrdU-immunoreactive cells in the dentate gyrus was measured by immunofluorescence staining. Our data showed the number of NR1-immunoreactive cells increased along with the decreasing numbers of BrdU- and Ki67-immunoreactive cells in the schizophrenia groups compared with the control group. N-methyl-D-aspartate could reverse the above changes. These results indicated that NR1 can regulate neurogenesis in the hippocampal dentate gyrus of schizophrenia mice, supporting NR1 as a promising therapeutic target in the treatment of schizophrenia. This study was approved by the Experimental Animal Ethics Committee of the Ningxia Medical University, China (approval No. 2014-014) on March 6, 2014. 展开更多
关键词 nerve REGENERATION SCHIZOPHRENIA MK-801 N-METHYL-D-ASPARTATE NEUROGENESIS N-METHYL-D-ASPARTATE receptor N-methyl-Daspartate receptor SUBUNIT 1 BrdU Ki67 HIPPOCAMPAL dentate gyrus HIPPOCAMPAL NEUROGENESIS neural REGENERATION
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涤痰汤糖尿病大鼠认知功能障碍的保护作用及机制 预览
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作者 杨光磊 王国强 +1 位作者 米佳 王秀阁 《世界中医药》 CAS 2019年第5期1163-1167,共5页
目的:观察涤痰汤对糖尿病大鼠认知能力的影响,并探讨其对PI3K-AKT信号通路相关因子的影响。方法:将30只SD鼠纳入研究,随机分为正常对照组、模型组及涤痰汤组,其中模型组及涤痰汤组大鼠均接受糖尿病模型制备,涤痰汤组用0.88 g/mL涤痰汤... 目的:观察涤痰汤对糖尿病大鼠认知能力的影响,并探讨其对PI3K-AKT信号通路相关因子的影响。方法:将30只SD鼠纳入研究,随机分为正常对照组、模型组及涤痰汤组,其中模型组及涤痰汤组大鼠均接受糖尿病模型制备,涤痰汤组用0.88 g/mL涤痰汤药液灌胃,正常对照组及模型组用等量的生理盐水灌胃,3组均连续灌胃28 d。各组采用水迷宫评估大鼠学习记忆功能,Tunel检测神经元细胞凋亡情况,HE染色法观察海马组织病理结构改变,Western blotting法检测各组海马组织PI3K、AKT、p-AKT、Bax及Bcl-2蛋白水平变化。结果:涤痰汤可明显改善糖尿病模型大鼠的学习记忆能力,抑制神经元凋亡率及Bax水平,增加海马区PI3K、p-AKT、Bcl-2蛋白表达。结论:涤痰汤可明显改善糖尿病模型大鼠的认知,其作用机制可能与介导PI3K/AKT信号通路有关。 展开更多
关键词 糖尿病模型 学习记忆能力 涤痰汤 水迷宫 海马 凋亡 PI3K-AKT信号通路 神经保护
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Dexmedetomidine reduces hippocampal microglia inflammatory response induced by surgical injury through inhibiting NLRP3
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作者 Ji Peng Peng Zhang +2 位作者 Han Zheng Yun-Qin Ren Hong Yan 《中华创伤杂志:英文版》 CAS CSCD 2019年第3期161-165,共5页
Purpose: To investigate whether dexmedetomidine (Dex) can reduce the production of inflammatory factor IL-1b by inhibiting the activation of NLRP3 inflammasome in hippocampal microglia, thereby alleviating the inflamm... Purpose: To investigate whether dexmedetomidine (Dex) can reduce the production of inflammatory factor IL-1b by inhibiting the activation of NLRP3 inflammasome in hippocampal microglia, thereby alleviating the inflammatory response of the central nervous system induced by surgical injury. Methods: Exploratory laparotomy was used in experimental models in this study. Totally 48 Sprague Dawley male rats were randomly divided into 4 groups (n = 12 for each), respectively sham control (group A), laparotomy only (group B);and Dex treatment with different doses of 5 mg/kg (group D1) or 10 mg/kg (group D2). Rats in groups D1 and D2 were intraperitoneally injected with corresponding doses of Dex every 6 h. The rats were sacrificed 12 h after operation;the hippocampus tissues were isolated, and frozen sections were made. The microglia activation was estimated by immunohistochemistry. The protein expression of NLRP3, caspase-1, ASC and IL-1b were detected by immunoblotting. All data were presented as mean ± standard deviation, and independent sample t test was used to analyze the statistical difference between groups. Results: The activated microglia in the hippocampus of the rats significantly increased after laparotomy (group B vs. sham control, p < 0.01). After Dex treatment, the number was decreased in a dosedependent way (group D1 vs. D2, p < 0.05), however the activated microglia in both groups were still higher than that of sham controls (both p < 0.05). Further Western blot analysis showed that the protein expression levels of NLRP3, caspase-1, ASC and downstream cytokine IL-1b in the hippocampus from the laparotomy group were significantly higher than those of the sham control group (all p < 0.01). The elevated expression of these proteins was relieved after Dex treatment, also in a dose-dependent way (D2 vs. D1 group, p < 0.05). Conclusion: Dex can inhibit the activation of microglia and NLRP3 inflammasome in the hippocampus of rats after operation, and the synthesis and secretion of IL-1b are also reduced in a 展开更多
关键词 DEXMEDETOMIDINE HIPPOCAMPAL MICROGLIA INFLAMMASOME IL-1β
Sodium butyrate prevents radiation-induced cognitive impairment by restoring pCREB/BDNF expression 预览
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作者 Hae June Lee Yeonghoon Son +6 位作者 Minyoung Lee Changjong Moon Sung Ho Kim In Sik Shin Miyoung Yang Sangwoo Bae Joong Sun Kim 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1530-1535,共6页
Sodium butyrate is a histone deacetylase inhibitor that affects various types of brain damages.To investigate the effects of sodium butyrate on hippocampal dysfunction that occurs after whole-brain irradiation in anim... Sodium butyrate is a histone deacetylase inhibitor that affects various types of brain damages.To investigate the effects of sodium butyrate on hippocampal dysfunction that occurs after whole-brain irradiation in animal models and the effect of sodium butyrate on radiation exposure-induced cognitive impairments,adult C57BL/6 mice were intraperitoneally treated with 0.6 g/kg sodium butyrate before exposure to 10 Gy cranial irradiation.Cognitive impairment in adult C57BL/6 mice was evaluated via an object recognition test 30 days after irradiation.We also detected the expression levels of neurogenic cell markers(doublecortin)and phosphorylated cAMP response element binding protein/brain-derived neurotrophic factor.Radiation-exposed mice had decreased cognitive function and hippocampal doublecortin and phosphorylated cAMP response element binding protein/brain-derived neurotrophic factor expression.Sodium butyrate pretreatment reversed these changes.These findings suggest that sodium butyrate can improve radiation-induced cognitive dysfunction through inhibiting the decrease in hippocampal phosphorylated cAMP response element binding protein/brain-derived neurotrophic factor expression.The study procedures were approved by the Institutional Animal Care and Use Committee of Korea Institute of Radiological Medical Sciences(approval No.KIRAMS16-0002)on December 30,2016. 展开更多
关键词 sodium BUTYRATE RADIOPROTECTOR ionizing radiation hippocampal damage cAMP response element binding BRAIN-DERIVED NEUROTROPHIC factor histone DEACETYLASE inhibitor neurogenesis
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胰酶稀释法提取乳鼠原代海马神经元 预览
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作者 康杨婷 王丽琨 伍国锋 《贵州医科大学学报》 CAS 2019年第1期59-63,共5页
目的:优化SD乳鼠海马神经元的提取方法。方法:选用出生24h内的SD乳鼠,分离双侧海马组织,分别用胰蛋白酶稀释法和未稀释法消化后获得细胞悬液;培养细胞,在培养第7天采用神经元特异性烯醇化酶(NSE)免疫荧光染色鉴定神经元数量及纯度。结果... 目的:优化SD乳鼠海马神经元的提取方法。方法:选用出生24h内的SD乳鼠,分离双侧海马组织,分别用胰蛋白酶稀释法和未稀释法消化后获得细胞悬液;培养细胞,在培养第7天采用神经元特异性烯醇化酶(NSE)免疫荧光染色鉴定神经元数量及纯度。结果:胰酶稀释组提取得到的海马神经元结构特征明显,能形成典型的神经细胞网络,90%以上海马神经细胞呈NSE阳性。结论:用稀释胰蛋白酶消化海马组织并配合特定培养条件可获得生长状态良好、纯度高的原代海马神经细胞。 展开更多
关键词 胰酶 稀释技术 神经元 海马 细胞培养 鉴定
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弥散张量成像测量海马体各向异性分数在帕金森病认知障碍中的应用 预览
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作者 陈旭辉 林志坚 +2 位作者 吴军 叶辰飞 郑晓东 《中风与神经疾病杂志》 CAS 2019年第1期7-9,共3页
目的使用磁共振弥散张量成像(DTI)观察帕金森病(PD)患者海马体各向异性分数(FA值)的变化特点,评估其作为诊断帕金森病认知障碍的应用价值。方法选取2015年1月~2015年12月在我院治疗的12个帕金森病患者(PD组)和18个年龄、性别相符的健康... 目的使用磁共振弥散张量成像(DTI)观察帕金森病(PD)患者海马体各向异性分数(FA值)的变化特点,评估其作为诊断帕金森病认知障碍的应用价值。方法选取2015年1月~2015年12月在我院治疗的12个帕金森病患者(PD组)和18个年龄、性别相符的健康志愿者(对照组)的海马体进行磁共振弥散张量成像,对两组的DTI参数各向异性分数(FA值)进行分析,比较两组海马FA值的差别。结果PD组的左侧、右侧和双侧平均海马体FA值(0.168±0.009、0.178±0.009、0.173±0.009),明显低于健康对照组(0.195±0.010、0.207±0.009、0.201±0.009,P<0.05).健康对照组和PD组的左侧海马体FA值均低于右侧海马体FA值(P<0.05)。结论利用海马体FA参数具有诊断帕金森病认知障碍的前景。 展开更多
关键词 帕金森病 各向异性参数 弥散张量成像 海马体
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适度跑轮运动对幼年小鼠学习记忆能力及海马神经发生的影响
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作者 石扬 李秉章 曹翠丽 《中华行为医学与脑科学杂志》 CAS CSCD 北大核心 2019年第4期322-325,共4页
目的观察适度跑轮运动对幼年小鼠学习记忆能力和海马神经发生的影响。方法取20只雄性1月龄昆明小鼠,随机分为2组:对照组和运动组。运动组经8周跑轮运动后,用Morris水迷宫实验检测两组小鼠空间学习和记忆能力;用免疫组织化学技术检测两... 目的观察适度跑轮运动对幼年小鼠学习记忆能力和海马神经发生的影响。方法取20只雄性1月龄昆明小鼠,随机分为2组:对照组和运动组。运动组经8周跑轮运动后,用Morris水迷宫实验检测两组小鼠空间学习和记忆能力;用免疫组织化学技术检测两组小鼠海马齿状回区反映神经发生的特异性蛋白Sox2、Ki67和DCX的表达情况。结果Morris水迷宫定位航行实验,运动组小鼠平均寻找平台潜伏期[(29.00±1.32)s]低于对照组[(36.30±0.69)s],差异有统计学意义(t=5.154,P<0.05)。空间探索实验,运动组穿越平台次数[(3.73±1.51)次]多于对照组[(1.89±1.63)次],差异有统计学意义(t=3.583,P<0.05)。免疫组织化学结果显示,运动组海马齿状回区Sox2、Ki67和DCX免疫阳性细胞数[分别为(284.40±31.50)个,(54.50±10.75)个,(77.80±11.60)个]多于对照组[分别为(241.40±10.57)个,(37.00±7.81)个,(48.20±11.86)个],均差异有统计学意义(t=4.129,5.789,7.971,均P<0.01)。结论适度跑轮运动可以显著提高幼年小鼠的学习记忆能力,可能与促进海马齿状回的神经发生有关。 展开更多
关键词 适度跑轮运动 MORRIS水迷宫实验 学习记忆 海马 神经发生 幼年小鼠
The detrimental effects of lipopolysaccharideinduced neuroinflammation on adult hippocampal neurogenesis depend on the duration of the pro-inflammatory response 预览
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作者 Martha Pérez-Domínguez Evangelina Aila-Munz +1 位作者 Eduardo Domínguez-Rivas Angélica Zepeda 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期817-825,共9页
Adult hippocampal neurogenesis is a finely tuned process regulated by extrinsic factors.Neuroinflammation is a hallmark of several pathological conditions underlying dysregulation of neurogenesis.In animal models,lipo... Adult hippocampal neurogenesis is a finely tuned process regulated by extrinsic factors.Neuroinflammation is a hallmark of several pathological conditions underlying dysregulation of neurogenesis.In animal models,lipopolysaccharide(LPS)-induced neuroinflammation leads to a neurogenic decrease mainly associated to the early inflammatory response.However,it is not well understood how the neuroinflammatory response progresses over time and if neurogenesis continues to be diminished during the late neuroinflammatory response.Moreover,it is unknown if repeated intermittent administration of LPS along time induces a greater reduction in neurogenesis.We administered one single intraperitoneal injection of LPS or saline or four repeated injections(one per week)of LPS or saline to young-adult mice.A cohort of new cells was labeled with three 5-bromo-2-deoxyuridine injections(one per day)4 days after the last LPS injection.We evaluated systemic and neuroinflammation-associated parameters and compared the effects of the late neuroinflammatory response on neurogenesis induced by each protocol.Our results show that 1)a single LPS injection leads to a late pro-inflammatory response characterized by microglial activation,moderate astrocytic reaction and increased interleukin-6 levels.This response correlates in time with decreased neurogenesis and 2)a repeated intermittent injection of LPS does not elicit a late pro-inflammatory response although activated microglia persists.The latter profile is not accompanied by a continued longterm hippocampal neurogenic decrease.Hereby,we provide evidence that the neuroinflammatory response is a dynamic process that progresses in a milieu-dependent manner and does not necessarily lead to a neurogenic decrease,highlighting the complex interaction between the immune system and neurogenesis. 展开更多
关键词 DENTATE GYRUS subgranular zone inflammation microglia ASTROCYTES IL-6 cytokines cell proliferation neural progenitor cells IMMATURE neurons long-term short-term adult hippocampal NEUROGENESIS
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Potassium bisperoxo(1,10-phenanthroline)oxovanadate suppresses proliferation of hippocampal neuronal cell lines by increasing DNA methyltransferases 预览
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作者 Xiao-Li Tian Shu-Yuan Jiang +7 位作者 Xiao-Lu Zhang Jie Yang Jun-He Cui Xiao-Lei Liu Ke-Rui Gong Shao-Chun Yan Chun-Yang Zhang Guo Shao 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期826-833,共8页
Bisperoxo(1,10-phenanthroline)oxovanadate(BpV)can reportedly block the cell cycle.The present study examined whether BpV alters gene expression by affecting DNA methyltransferases(DNMTs),which would impact the cell cy... Bisperoxo(1,10-phenanthroline)oxovanadate(BpV)can reportedly block the cell cycle.The present study examined whether BpV alters gene expression by affecting DNA methyltransferases(DNMTs),which would impact the cell cycle.Immortalized mouse hippocampal neuronal precursor cells(HT22)were treated with 0.3 or 3μM BpV.Proliferation,morphology,and viability of HT22 cells were detected with an IncuCyte real-time video imaging system or inverted microscope and 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium,respectively.mRNA and protein expression of DNMTs and p21 in HT22 cells was detected by real-time polymerase chain reaction and immunoblotting,respectively.In addition,DNMT activity was measured with an enzyme-linked immunosorbent assay.Effects of BpV on the cell cycle were analyzed using flow cytometry.Results demonstrated that treatment with 0.3μM BpV did not affect cell proliferation,morphology,or viability;however,treatment with 3μM BpV decreased cell viability,increased expression of both DNMT3B mRNA and protein,and inhibited the proliferation of HT22 cells;and 3μM BpV also blocked the cell cycle and increased expression of the regulatory factor p21 by increasing DNMT expression in mouse hippocampal neurons. 展开更多
关键词 nerve REGENERATION hippocampal neurons POTASSIUM bisperoxo(1 10-phenanthroline)oxovanadate DNA METHYLTRANSFERASE p21 HT22 CELL CELL cycle immunoblotting DNA methylation neural REGENERATION
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Predator stress-induced depression is associated with inhibition of hippocampal neurogenesis in adult male mice 预览
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作者 Yan-Ping Wu Hua-Ying Gao +3 位作者 Shu-Hua Ouyang Hiroshi Kurihara Rong-Rong He Yi-Fang Li 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第2期298-305,共8页
Stress has been suggested to disturb the 5-hydroxytryptamine system and decrease neurogenesis, which contribute to the development of depression. Few studies have investigated the effect of predator stress, a type of ... Stress has been suggested to disturb the 5-hydroxytryptamine system and decrease neurogenesis, which contribute to the development of depression. Few studies have investigated the effect of predator stress, a type of psychological stress, on depression and hippocampal neurogenesis in adult mice; we therefore investigated this in the present study. A total of 35 adult male Kunming mice were allocated to a cat stress group, cat odor stress group, cat stress + fluoxetine group, cat odor stress + fluoxetine group, or a control group (no stress/treatment). After 12 days of cat stress or cat odor stress, behavioral correlates of depression were measured using the open field test, elevated plus maze test, and dark-avoidance test. The concentrations of hippocampal 5-hydroxytryptamine and 5-hydroxyindoleacetic acid were measured using high-performance liquid chromatography-electrochemical detection. Neurogenesis was also analyzed using a bromodeoxyuridine and doublecortin double-immunostaining method. Cat stress and cat odor stress induced depression-like behaviors; this effect was stronger in the cat stress model. Furthermore, compared with the control group, cat stress mice exhibited lower 5-hydroxytryptamine concentrations, higher 5-hydroxyindoleacetic acid concentrations, and significantly fewer bromodeoxyuridine+/doublecortin+-labeled cells in the dentate gyrus, which was indicative of less neurogenesis. The changes observed in the cat stress group were not seen in the cat stress + fluoxetine group, which suggests that the effects of predator stress on depression and neurogenesis were reversed by fluoxetine. Taken together, our results indicate that depression-like behaviors induced by predator stress are associated with the inhibition of hippocampal neurogenesis. 展开更多
关键词 nerve REGENERATION DEPRESSIVE disorder CAT STRESS CAT odor STRESS behavioral evaluation open field TEST elevated plus maze TEST dark-avoidance TEST 5-HYDROXYTRYPTAMINE 5-hydroxyindoleacetic acid hippocampal neurogenesis neural REGENERATION
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七氟醚对大鼠海马组织NR2B、Caspase-3的影响 预览
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作者 朱彦东 刘钰 《重庆医学》 CAS 2019年第13期2189-2191,2196共4页
目的探讨七氟醚对大鼠海马组织N-甲基-D-天冬氨酸-2B受体(NR2B)、半胱氨酸蛋白酶3(Caspase-3)的影响。方法选取7d龄SD大鼠60只分为对照组和麻醉组,每组再分为3个时间点,即麻醉前、麻醉后24h及麻醉后7周,每组10只。麻醉组给予2.5%七氟醚... 目的探讨七氟醚对大鼠海马组织N-甲基-D-天冬氨酸-2B受体(NR2B)、半胱氨酸蛋白酶3(Caspase-3)的影响。方法选取7d龄SD大鼠60只分为对照组和麻醉组,每组再分为3个时间点,即麻醉前、麻醉后24h及麻醉后7周,每组10只。麻醉组给予2.5%七氟醚麻醉4h,对照组仅给予空氧混合气。采用Western blot检测各组大鼠海马组织NR2B、Caspase-3蛋白表达,Morris水迷宫检测麻醉后7周大鼠的空间学习记忆能力。结果麻醉后24h,麻醉组大鼠海马组织NR2B、Caspase-3蛋白表达明显高于对照组及麻醉前(P<0.05);麻醉前及麻醉后7周,麻醉组和对照组大鼠海马组织NR2B、Caspase-3蛋白表达比较差异无统计学意义(P>0.05)。麻醉组大鼠第1、3天游泳潜伏期分别为(70.23±8.10)s和(41.03±7.39)s,总路程分别为(18.20±2.02)cm和(11.03±1.98)cm,明显高于对照组(P<0.05);麻醉组和对照组大鼠游泳速度比较差异无统计学意义(P>0.05)。结论七氟醚麻醉可即刻造成幼鼠海马组织NR2B、Caspase-3蛋白表达升高,同时其成年后的学习记忆能力有一定损伤,但成年后海马组织NR2B、Caspase-3蛋白表达无明显异常。 展开更多
关键词 七氟醚 大鼠 Sprague-Dawley 海马 NR2B 半胱氨酸天冬氨酸蛋白酶3
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血管性痴呆大鼠海马神经元LC3和Beclin-1的表达 被引量:1
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作者 高健 王德秀 +1 位作者 王凤斌 王玉良 《神经解剖学杂志》 CSCD 北大核心 2018年第4期467-472,共6页
目的:在血管性痴呆(vascular dementia,VD)模型大鼠,观察自噬相关蛋白LC3和Beclin-1在海马CA1区神经元中的表达变化情况。方法:采用重复夹闭双侧颈总动脉(CCA)同时腹腔注射硝普钠溶液方法制备VD大鼠模型,在1、3、5和7 d四个时间点... 目的:在血管性痴呆(vascular dementia,VD)模型大鼠,观察自噬相关蛋白LC3和Beclin-1在海马CA1区神经元中的表达变化情况。方法:采用重复夹闭双侧颈总动脉(CCA)同时腹腔注射硝普钠溶液方法制备VD大鼠模型,在1、3、5和7 d四个时间点,分别采用Morris水迷宫检测大鼠空间学习记忆能力;HE染色观察CA1区神经元形态变化情况;免疫组化和Western Blot观察海马CA1区神经元上LC3和Beclin-1的表达情况。结果:模型组大鼠各时间点的逃逸潜伏期(escape latency,EL)比假手术组均明显延长(P〈0.01)。HE染色结果发现,与假手术组相比,模型组大鼠海马神经元出现严重损伤,且随时间延长逐渐加重。免疫组化和Western Blot积分光密度值测定结果显示,模型组大鼠各时间点LC3和Beclin-1的表达均比假手术组明显升高(P〈0.01),5 d时间点达到高峰。结论:VD模型大鼠缺血再灌注短时间内海马CA1区神经元出现严重损伤,伴有自噬相关的LC3和Beclin-1一过性表达升高。 展开更多
关键词 VD模型 自噬 LC3 BECLIN-1 海马
Dedicated Magnetic Resonance Imaging Sequences:Contribution in the Diagnosis of Focal Epilepsy in the Lebanese Population 预览
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作者 Najo A.Jomaa Marwan Haddad +2 位作者 Grace Y.Adwane Amira J.Zaylaa Abdallah Rahbani 《健康科学:英文版》 2018年第6期439-445,共7页
There have been many advances in the diagnosis and management of focal epilepsies particularly with neuroimaging techniques and magnetic resonance imaging(MRI).Special MRI sequences can be employed to localize and res... There have been many advances in the diagnosis and management of focal epilepsies particularly with neuroimaging techniques and magnetic resonance imaging(MRI).Special MRI sequences can be employed to localize and resect the epileptogenic lesions responsible for focal epilepsy.This study aims to show the benefit of dedicated epilepsy MRI sequences in the diagnosis of focal epilepsies.A general electric 1.5 Tesla MRI machine was used with standard and special sequences.Also,a Nihon Kohden electroencephalography(EEG)machine was used.This is a prospective observational study that included 51 patients with focal epilepsies who had an initial negative brain imaging.They underwent epilepsy MRI sequences along with a prolonged video EEG monitoring to localize the lesion,and then results were analyzed statistically using SPSS 22 program.The majority of patients were males(62.75%)with a mean age of 30 years.The grand majority of patients(74.5%,p value of 0.001)had their lesion localized by the epilepsy MRI.The most commonly found pathology was mesial temporal sclerosis.A significant number of patients(23.5%)were sent for an epilepsy surgery(p value 0.002).This study shows the significant impact of dedicated epilepsy MRI sequences on the diagnosis and management of focal epilepsy in the Lebanese population. 展开更多
关键词 Epilepsy magnetic resonance imaging DEDICATED SEQUENCES focal epileptogenic lesions hippocampal SCLEROSIS CORTICAL dysplasia.
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温阳补肾灸对血管性痴呆大鼠NF-κB信号通路的影响
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作者 朱婉丽 杨坤 +2 位作者 蔡圣朝 王莹 宋小鸽 《世界针灸杂志:英文版》 CSCD 2018年第1期44-49,I0006,I0007共8页
目的:观察温阳补肾灸对血管性痴呆(VD)大鼠行为学、海马IL-1β、TNF-α及NF-κB相关基因与蛋白表达的影响,探讨温阳补肾灸抑制VD炎性反应的机制。方法:SD大鼠随机分为假手术组、模型组、艾灸组、西药组,每组10只。采用双侧颈总动脉... 目的:观察温阳补肾灸对血管性痴呆(VD)大鼠行为学、海马IL-1β、TNF-α及NF-κB相关基因与蛋白表达的影响,探讨温阳补肾灸抑制VD炎性反应的机制。方法:SD大鼠随机分为假手术组、模型组、艾灸组、西药组,每组10只。采用双侧颈总动脉缺血再灌注法制造VD大鼠模型。造模后,艾灸组大鼠悬灸"大椎""命门""关元",每次15 min,每日一次,连续治疗4周;西药组大鼠用尼莫地平灌胃(2 mg·kg-1·d-1)连续治疗4周。运用Morris水迷宫测试对各组大鼠进行行为学检测。用HE染色观察其组织病理学改变。运用RT-qPCR法和Westernblot法检测海马IL-1β、TNF-α及NF-κB相关蛋白与基因的表达。结果:造模后手术组大鼠Morris水迷宫逃避潜伏期较假手术组明显延长(P〈0.05)。HE染色显示模型组神经元细胞排列松散,细胞膜模糊,细胞质不均匀,核固缩,坏死量增多,炎细胞显著增加。与模型组相比,艾灸组与西药组炎细胞数目明显减少,神经元坏死减少。治疗后,与模型组比较,艾灸组、西药组大鼠海马组织TNF-α、p-IκB、NF-κBp65蛋白表达量显著降低(P〈0.05);与模型组比较,艾灸组、西药组大鼠海马组织IL-1β、TNF-α、NF-κBp65 mRNA表达显著降低(P〈0.05)。结论:温阳补肾灸可能通过降低脑内IL-1β、TNF-α及NF-κB相关基因的表达水平,抑制NF-κB信号通路,降低炎性反应,以达到治疗VD的目的。 展开更多
关键词 艾灸 血管性痴呆大鼠 炎症反应 NF-ΚB信号通路
Distinct effect of potassium 2-(l-hydroxypentyl) - benzoate on hippocampal neurons, synapses and dystrophic axons in APP/PS1 mice
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作者 HUANG Long-jian ZHANG Yong +2 位作者 LAN Jia-qi WANG Xiao-liang PENG Ying 《中国药理学与毒理学杂志》 CSCD 北大核心 2018年第9期691-692,共2页
OBJECTIVE To study the protective effect of potassium 2-(l-hydroxypentyl)-benzoate (PHPB) on hippocampal neurons, synapses and dystrophic axons in APP/PS1 mice. METHODS Ten-month-old male APP/PS1 transgenic mice and a... OBJECTIVE To study the protective effect of potassium 2-(l-hydroxypentyl)-benzoate (PHPB) on hippocampal neurons, synapses and dystrophic axons in APP/PS1 mice. METHODS Ten-month-old male APP/PS1 transgenic mice and age-matched wild-type mice were randomly divided into three groups: wild-type group (WT Con group, n =10), APP / PS1 group (Tg Con group, n=10) and PHPB treated APP / PS1 group (PHPB group, n= 10). PHPB group received 30 mg·kg^-1 PHPB by oral gavage once daily for 3 months. WT Con group and Tg Con group received the same volume of water. Three months later, mice were sacrificed for biochemical and pathological testing such as transmission electron microscopy, Golgi staining and Western boltting analysis. RESULTS Under the transmission electron microscope, most hippocampal neurons and subcellular organelles in WT Con group exhibited normal morphology. However, the degenerative changes were observed in Tg Con group such as nuclear fragmentation, mitochondrial swelling, ribosomes detachment and autophagic vacuoles accumulation. The hippocampal synapses number and the thickness of postsynaptic density (PSD) were significantly decreased in Tg Con group compared with the WT Con group (P< 0.05). After PHPB treatment, the degenerative changes in APP/PS1 mice were alleviated to some extent. The synapse number has been elevated significantly (P<0.05) and the PSD has been thickened as well. Golgi staining showed that the spine density of secondary and tertiary apical dendritic branches was significantly decreased in CA1 and DG areas of Tg Con group (P<0.05). Sholl analysis revealed a decrease of dendritic complexity in Tg Con group compared with WT Con group (P<0.05). These abnormalities were alleviated to some extent after PHPB treatment. Western blotting study showed that the protein levels of synaptic marker PSD-95 and synaptophysin were significantly decreased in the hippocampus of Tg Con group (P<0.05). A significant increase of PSD-95 (P<0.05) and a slight increase of SYP were observed after the PHPB trea 展开更多
关键词 POTASSIUM 2-(l-hydroxypentyl)-benzoate APP/PS1 MICE hippocampal SYNAPSE dysfunction dystrophic AXONS
伴双侧海马病变的LGI 1-Ab型自身免疫性脑炎1例
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作者 侯思佳 赵继巍 +2 位作者 段淑荣 于宏丽 赵敬堃 《神经疾病与精神卫生》 2018年第9期682-684,共3页
富亮氨酸胶质瘤活基因1(leucine-rich glioma inactivated gene-1, LGI 1)-Ab型脑炎是一种近年来新发现的神经元表面抗体综合征,是一种由LGI 1抗体参与致病的自身免疫性脑炎.作为电压门控钾通道复合体(voltage gated potassium chann... 富亮氨酸胶质瘤活基因1(leucine-rich glioma inactivated gene-1, LGI 1)-Ab型脑炎是一种近年来新发现的神经元表面抗体综合征,是一种由LGI 1抗体参与致病的自身免疫性脑炎.作为电压门控钾通道复合体(voltage gated potassium channel complex, VGKC) 抗体脑炎最常见的类型,除具有边缘叶脑炎常见的认知障碍及癫痫和精神症状外,还具有特征性的面-臂肌张力障碍发作(faciobrachial dystonic seizure,FBDS)和顽固性低钠血症,免疫治疗效果较好.现对1例LGI 1-Ab型自身免疫性脑炎的病例进行分析,报道如下. 展开更多
关键词 认知障碍 癫痫 自身免疫性脑炎 海马
Real-time Microwave Exposure Induces Calcium Efflux in Primary Hippocampal Neurons and Primary Cardiomyocytes 预览
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作者 WANG Hui ZHANG Jing +4 位作者 HU Shao Hua TAN Sheng Zhi ZHANG Bo ZHOU Hong Mei PENG Rui Yun 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2018年第8期561-571,共11页
Objective To detect the effects of microwave on calcium levels in primary hippocampal neurons and primary cardiomyocytes by the real-time microwave exposure combined with laser scanning confocal microscopy. Methods Th... Objective To detect the effects of microwave on calcium levels in primary hippocampal neurons and primary cardiomyocytes by the real-time microwave exposure combined with laser scanning confocal microscopy. Methods The primary hippocampal neurons and primary cardiomyocytes were cultured and labeled with probes, including Fluo-4 AM, Mag-Fluo-AM, and Rhod-2, to reflect the levels of whole calcium [Ca2+], endoplasmic reticulum calcium [Ca2+]ER, and mitochondrial calcium [Ca2+]MIT, respectively. Then, the cells were exposed to a pulsed microwave of 2.856 GHz with specific absorption rate (SAR) values of 0, 4, and 40 W/kg for 6 min to observe the changes in calcium levels. Results The results showed that the 4 and 40 W/kg microwave radiation caused a significant decrease in the levels of [Ca2+], [Ca2+]ER, and [Ca2+]MIT in primary hippocampal neurons. In the primary cardiomyocytes, only the 40 W/kg microwave radiation caused the decrease in the levels of [Ca2+], [Ca2+]ER, and [Ca2+]MIT. Primary hippocampal neurons were more sensitive to microwave exposure than primary cardiomyocytes. The mitochondria were more sensitive to microwave exposure than the endoplasmic reticulum. Conclusion The calcium efflux was occurred during microwave exposure in primary hippocampal neurons and primary cardiomyocytes. Additionally, neurons and mitochondria were sensitive cells and organelle respectively. 展开更多
关键词 Real time Microwave CALCIUM PRIMARY HIPPOCAMPAL neurons PRIMARY CARDIOMYOCYTES
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Artemisinin protected human SY5Y and hippocampal neurons from H2O2-induced oxidative damage through activation of AMPK pathway
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作者 JIANG Yi-zhou ZHAO Xia ZHENG Wen-hua 《中国药理学与毒理学杂志》 CSCD 北大核心 2018年第9期703-704,共2页
Oxidative stress is one of the main causes of neurodegenerative diseases such as Alzheimer disease (AD). Our previous studies have shown that artemisinin, a anti-malaria Chinese medicine, with neuroprotective effect, ... Oxidative stress is one of the main causes of neurodegenerative diseases such as Alzheimer disease (AD). Our previous studies have shown that artemisinin, a anti-malaria Chinese medicine, with neuroprotective effect, however, the antioxidative effect of artemisinin and its potential mechanism remain to be elucidated. In the present study, the protective effect and the underlying mechanism of artemisinin against injury of hydrogen peroxide (H2O2) in SH-SY5Y and hippocampal neurons were studied. Our results show that artemisinin protected SH-SY5Y and hippocampal neuronal cells from H2O2-induced cell death at clinically relevant concentrations in a concentration-dependent manner. Further studies showed that artemisinin significantly reduced cell death caused by H2O2 by restoring nuclear morphology, abnormal changes in intracellular ROS, activation of caspase 3, lactate dehydrogenase release and mitochondrial membrane potential. Hoechst staining and flow cytometry showed that artemisinin significantly reduced the apoptosis of SH-SY5Y cells exposed to H2O2. Western blotting analysis showed that artemisinin stimulated the phosphorylation and activation of AMP-activated protein kinase (AMPK) in SH-SY5Y cells in a time and concentration-dependent manner, whereas the application of AMPK inhibitor Compound C or decrease in expression of AMPKα with shRNA specific for AMPKα blocked the protective effect of artemisinin. Similar results were obtained in primary cultured hippocampal neurons. Taken together, these results indicate that artemisinin can protect neuronal cells from oxidative damage, at least in part through the activation of AMPK. Because artemisinin is relatively inexpensive and has few side effects, our findings support the role of artemisinin as a potential therapeutic agent for neurodegenerative diseases. 展开更多
关键词 ARTEMISININ hydrogen PEROXIDE SHSY5Y cells HIPPOCAMPAL neurons AMPK PATHWAY
GABAA Receptor Activity Suppresses the Transition from Inter- ictal to Ictal Epileptiform Discharges in Juvenile Mouse Hippocampus
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作者 Yan-Yan Chang Xin-Wei Gong +3 位作者 Hai-Qing Gong Pei-Ji Liang Pu-Ming Zhang Qin-Chi Lu 《神经科学通报:英文版》 SCIE CAS CSCD 2018年第6期1007-1016,共10页
Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment.We ... Exploring the transition from inter-ictal to ictal epileptiform discharges(IDs) and how GABAAreceptormediated action affects the onset of IDs will enrich our understanding of epileptogenesis and epilepsy treatment.We used Mg2+-free artificial cerebrospinal fluid(ACSF) to induce epileptiform discharges in juvenile mouse hippocampal slices and used a micro-electrode array to record the discharges. After the slices were exposed to Mg2+-free ACSF for 10 min–20 min, synchronous recurrent seizurelike events were recorded across the slices, and each event evolved from inter-ictal epileptiform discharges(IIDs) to pre-ictal epileptiform discharges(PIDs), and then to IDs.During the transition from IIDs to PIDs, the duration of discharges increased and the inter-discharge interval decreased. After adding 3 lmol/L of the GABAAreceptor agonist muscimol, PIDs and IDs disappeared, and IIDs remained. Further, the application of 10 lmol/L muscimol abolished all the epileptiform discharges. When the GABAAreceptor antagonist bicuculline was applied at 10 lmol/L, IIDs and PIDs disappeared, and IDs remained at decreased intervals. These results indicated that there are dynamic changes in the hippocampal network preceding the onset of IDs, and GABAAreceptor activity suppresses the transition from IIDs to IDs in juvenile mouse hippocampus. 展开更多
关键词 EPILEPTIFORM discharge Gamma-aminobutyric acid BICUCULLINE MUSCIMOL Micro-electrode array Hippocampal slice
螺旋藻对运动疲劳大鼠海马损伤的保护作用及机制研究 预览
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作者 朱洪竹 张莹 +2 位作者 朱梅菊 伍人乐 曾志刚 《中国应用生理学杂志》 CAS CSCD 北大核心 2018年第6期562-567,共6页
目的:研究BDNF/Trk B神经营养信号在运动疲劳大鼠海马神经元损伤中的作用与螺旋藻改善运动致脑海马损伤的作用及其可能的机制。方法:60只雄性SD大鼠随机分为:正常对照组(NC组)、正常+螺旋藻灌胃组(NS组)、运动模型组(EM组)、运动+螺旋... 目的:研究BDNF/Trk B神经营养信号在运动疲劳大鼠海马神经元损伤中的作用与螺旋藻改善运动致脑海马损伤的作用及其可能的机制。方法:60只雄性SD大鼠随机分为:正常对照组(NC组)、正常+螺旋藻灌胃组(NS组)、运动模型组(EM组)、运动+螺旋藻灌胃组(ES组)、阳性对照组(PC组),每组12只。EM组、ES组和PC组采用3周的递增式跑台训练建立运动疲劳模型。NC组不施加任何干预,用作对照。NS组和ES组按每天300mg/kg体重灌胃螺旋藻,PC组以同等体积的人参提取物(1.92 g/kg)灌胃,连续灌胃3周。实验末,用免疫组化和免疫印迹法检测各组大鼠海马BDNF、Trk B、p-TrkB蛋白表达水平,并用尼氏染色法观察海马CA1区形态结构的变化,同时观察大鼠体重等一般情况。结果:与NC组比较,EM组大鼠的体重降低,海马CA1区神经元细胞形态异常且排列紊乱,部分细胞固缩呈不规则变化,部分神经元消失不见,海马BDNF、Trk B和p-TrkB蛋白表达均明显升高(P<0.01);与EM组比较,ES组大鼠的体重增加,海马神经元损伤得到明显改善,神经元数目和尼氏小体数量增加,神经元排列渐趋规则,形态较完整,ES组海马BDNF、Trk B和p-TrkB蛋白表达均明显升高(P<0.05或P<0.01),且与PC组相比,已无明显差别(P>0.05)。结论:BDNF/Trk B神经营养信号可能参与运动致疲劳大鼠海马神经元损伤的修复过程;螺旋藻补充能改善运动疲劳大鼠海马神经元损伤,其原因可能与其上调BDNF和其受体(Trk B)及其受体磷酸化(p-TrkB)蛋白表达而发挥神经保护作用有关。 展开更多
关键词 螺旋藻 BDNF/Trk B通路蛋白 海马 大鼠 运动疲劳
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