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Differences in pathological changes between two rat models of severe traumatic brain injury 预览
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作者 Yi-Ming Song Yu Qian +6 位作者 Wan-Qiang Su Xuan-Hui Liu Jin-Hao Huang Zhi-Tao Gong Hong-Liang Luo Chuang Gao Rong-Cai Jiang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1796-1804,共9页
The rat high-impact free weight drop model mimics the diffuse axonal injury caused by severe traumatic brain injury in humans,while severe controlled cortical impact can produce a severe traumatic brain injury model u... The rat high-impact free weight drop model mimics the diffuse axonal injury caused by severe traumatic brain injury in humans,while severe controlled cortical impact can produce a severe traumatic brain injury model using precise strike parameters.In this study,we compare the pathological mechanisms and pathological changes between two rat severe brain injury models to identify the similarities and differences.The severe controlled cortical impact model was produced by an electronic controlled cortical impact device,while the severe free weight drop model was produced by dropping a 500 g free weight from a height of 1.8 m through a plastic tube.Body temperature and mortality were recorded,and neurological deficits were assessed with the modified neurological severity score.Brain edema and bloodbrain barrier damage were evaluated by assessing brain water content and Evans blue extravasation.In addition,a cytokine array kit was used to detect inflammatory cytokines.Neuronal apoptosis in the brain and brainstem was quantified by immunofluorescence staining.Both the severe controlled cortical impact and severe free weight drop models exhibited significant neurological impairments and body temperature fluctuations.More severe motor dysfunction was observed in the severe controlled cortical impact model,while more severe cognitive dysfunction was observed in the severe free weight drop model.Brain edema,inflammatory cytokine changes and cortical neuronal apoptosis were more substantial and blood-brain barrier damage was more focal in the severe controlled cortical impact group compared with the severe free weight drop group.The severe free weight drop model presented with more significant apoptosis in the brainstem and diffused blood-brain barrier damage,with higher mortality and lower repeatability compared with the severe controlled cortical impact group.Severe brainstem damage was not found in the severe controlled cortical impact model.These results indicate that the severe controlled cortical impact model is relat 展开更多
关键词 nerve REGENERATION severe traumatic brain INJURY animal model comparison free weight drop controlled cortical impact NEUROLOGICAL impairment NEUROINFLAMMATION blood-brain barrier damage neuronal apoptosis diffuse AXONAL INJURY BRAINSTEM INJURY neural REGENERATION
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Independent lung ventilation:Implementation strategies and review of literature 预览
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作者 Sheri Berg Edward A Bittner +2 位作者 Lorenzo Berra Robert M Kacmarek Abraham Sonny 《世界重症医学杂志》 2019年第4期49-58,共10页
Independent lung ventilation,though infrequently used in the critical care setting,has been reported as a rescue strategy for patients in respiratory failure resulting from severe unilateral lung pathology.This involv... Independent lung ventilation,though infrequently used in the critical care setting,has been reported as a rescue strategy for patients in respiratory failure resulting from severe unilateral lung pathology.This involves isolating and ventilating the right and left lung differently,using separate ventilators.Here,we describe our experience with independent lung ventilation in a patient with unilateral diffuse alveolar hemorrhage,who presented with severe hypoxemic respiratory failure despite maximal ventilatory support.Conventional ventilation in this scenario leads to preferential distribution of tidal volume to the nondiseased lung causing over distension and inadvertent volume trauma.Since each lung has a different compliance and respiratory mechanics,instituting separate ventilation strategies to each lung could potentially minimize lung injury.Based on review of literature,we provide a detailed description of indications and procedures for establishing independent lung ventilation,and also provide an algorithm for management and weaning a patient from independent lung ventilation. 展开更多
关键词 UNILATERAL LUNG INJURY UNILATERAL PNEUMONIA Double LUMEN tube Differential LUNG ventilation Acute LUNG INJURY VENTILATOR induced LUNG INJURY
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Serum Copeptin and In-Hospital Major Adverse Events after Head Trauma: A Multicenter Study 预览
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作者 Yu-Hao Shen Quan Du +11 位作者 Wen-Hua Yu Xiao-Qiao Dong Ding-Bo Yang Jian-Wei Pan Jian-Feng Weng Li-Feng Luo Meng Cen Zu-Yong Zhang Yong-Ke Zheng Wei Hu Li-Xin Zhang Mao-Fa Xu 《脑血管病电子杂志(英文)》 2019年第1期15-24,共10页
Background: Copeptin is a biomarker for brain injury. However,it is unclear whether it is discriminative regarding in-hospital major adverse events (IMAEs),including acute lung injury,acute traumatic coagulopathy,prog... Background: Copeptin is a biomarker for brain injury. However,it is unclear whether it is discriminative regarding in-hospital major adverse events (IMAEs),including acute lung injury,acute traumatic coagulopathy,progressive hemorrhagic injury and post-traumatic cerebral infarction,in patients with traumatic brain injury (TBI). This study was aimed at investigating the relationship between the serum copeptin level and occurrences of IMAEs. Methods: In this multicenter observational study,we recruited 173 severe TBI patients and 173 healthy controls. Serum levels of copeptin,interleukin-6,tumor necrosis factor-alpha,C-reactive protein,myelin basic protein,glial fibrillary astrocyte protein,S100B,neuron-specific enolase,phosphorylated axonal neurofilament subunit H,tau protein and ubiquitin carboxyl-terminal hydrolase L1 were quantified using available enzyme-linked immunosorbent assays. Areas under the receiver operating characteristic curves (AUCs) were estimated to determine and compare their discriminatory ability for IMAEs. Results: Patients had dramatically elevated levels of the afore-mentioned biomarkers,as compared with controls. In the discrimination of IMAEs,serum copeptin had a significantly higher AUC than serum interleukin-6,tumor necrosis factor-alpha and C-reactive protein and its AUC was similar to that of the Glasgow coma scale (GCS) score and the other remaining biomarkers mentioned above. Except copeptin,no other biomarkers could significantly improve the AUC of the GCS score. Conclusion: Serum copeptin levels combined with the GCS score might aid in discriminating IMAEs following TBI. 展开更多
关键词 TRAUMATIC brain INJURY COPEPTIN ACUTE lung INJURY ACUTE TRAUMATIC COAGULOPATHY ACUTE progressive hemorrhagic INJURY POST-TRAUMATIC cerebral infarction
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The pig as a preclinical traumatic brain injury model: current models,functional outcome measures,and translational detection strategies 预览
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作者 Holly A.Kinder Emily W.Baker Franklin D.West 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第3期413-424,共12页
Traumatic brain injury(TBI) is a major contributor of long-term disability and a leading cause of death worldwide. A series of secondary injury cascades can contribute to cell death, tissue loss, and ultimately to the... Traumatic brain injury(TBI) is a major contributor of long-term disability and a leading cause of death worldwide. A series of secondary injury cascades can contribute to cell death, tissue loss, and ultimately to the development of functional impairments. However, there are currently no effective therapeutic interventions that improve brain outcomes following TBI. As a result, a number of experimental TBI models have been developed to recapitulate TBI injury mechanisms and to test the efficacy of potential therapeutics. The pig model has recently come to the forefront as the pig brain is closer in size, structure, and composition to the human brain compared to traditional rodent models, making it an ideal large animal model to study TBI pathophysiology and functional outcomes. This review will focus on the shared characteristics between humans and pigs that make them ideal for modeling TBI and will review the three most common pig TBI models–the diffuse axonal injury, the controlled cortical impact, and the fluid percussion models. It will also review current advances in functional outcome assessment measures and other non-invasive, translational TBI detection and measurement tools like biomarker analysis and magnetic resonance imaging. The use of pigs as TBI models and the continued development and improvement of translational assessment modalities have made significant contributions to unraveling the complex cascade of TBI sequela and provide an important means to study potential clinically relevant therapeutic interventions. 展开更多
关键词 traumatic brain INJURY large animal MODEL PIG MODEL diffuse AXONAL INJURY FUNCTIONAL outcome assessment measures controlled cortical impact MODEL fluid percussion INJURY MODEL magnetic resonance imaging biomarkers
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Contralateral C7 transfer combined with acellular nerve allografts seeded with differentiated adipose stem cells for repairing upper brachial plexus injury in rats 预览
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作者 Jian-Tao Yang Jin-Tao Fang +3 位作者 Liang Li Gang Chen Ben-Gang Qin Li-Qiang Gu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1932-1940,共9页
Nerve grafting has always been necessary when the contralateral C7 nerve root is transferred to treat brachial plexus injury. Acellular nerve allograft is a promising alternative for the treatment of nerve defects, an... Nerve grafting has always been necessary when the contralateral C7 nerve root is transferred to treat brachial plexus injury. Acellular nerve allograft is a promising alternative for the treatment of nerve defects, and results were improved by grafts laden with differentiated adipose stem cells. However, use of these tissue-engineered nerve grafts has not been reported for the treatment of brachial plexus injury. The aim of the present study was to evaluate the outcome of acellular nerve allografts seeded with differentiated adipose stem cells to improve nerve regeneration in a rat model in which the contralateral C7 nerve was transferred to repair an upper brachial plexus injury. Differentiated adipose stem cells were obtained from Sprague-Dawley rats and transdifferentiated into a Schwann cell-like phenotype. Acellular nerve allografts were prepared from 15-mm bilateral sections of rat sciatic nerves. Rats were randomly divided into three groups: acellular nerve allograft, acellular nerve allograft + differentiated adipose stem cells, and autograft. The upper brachial plexus injury model was established by traction applied away from the intervertebral foramen with micro-hemostat forceps. Acellular nerve allografts with or without seeded cells were used to bridge the gap between the contralateral C7 nerve root and C5–6 nerve. Histological staining, electrophysiology, and neurological function tests were used to evaluate the effect of nerve repair 16 weeks after surgery. Results showed that the onset of discernible functional recovery occurred earlier in the autograft group first, followed by the acellular nerve allograft + differentiated adipose stem cells group, and then the acellular nerve allograft group;moreover, there was a significant difference between autograft and acellular nerve allograft groups. Compared with the acellular nerve allograft group, compound muscle action potential, motor conduction velocity, positivity for neurofilament and S100, diameter of regenerating axons, myelin sheath thickness, 展开更多
关键词 NERVE REGENERATION peripheral NERVE INJURY brachial plexus INJURY CONTRALATERAL C7 NERVE root acellular NERVE adipose stem CELLS Schwann CELLS tissue engineering NERVE NERVE grafting NERVE defect neural REGENERATION
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Mechanism of persistent hyperalgesia in neuropathic pain caused by chronic constriction injury 预览
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作者 Qin-Yi Chen Chao-Yang Tan +3 位作者 Yang Wang Ke-Tao Ma Li Li Jun-Qiang Si 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期1091-1098,共8页
Transmembrane member 16A(TMEM16A)is involved in many physiological functions,such as epithelial secretion,sensory conduction,nociception,control of neuronal excitability,and regulation of smooth muscle contraction,and... Transmembrane member 16A(TMEM16A)is involved in many physiological functions,such as epithelial secretion,sensory conduction,nociception,control of neuronal excitability,and regulation of smooth muscle contraction,and may be important in peripheral pain transmission.To explore the role of TMEM16A in the persistent hyperalgesia that results from chronic constriction injury-induced neuropathic pain,a rat model of the condition was established by ligating the left sciatic nerve.A TMEM16A selective antagonist(10μg T1 6Ainh-A01)was intrathecally injected at L5–6.For measurement of thermal hyperalgesia,the drug was administered once at 14 days and thermal withdrawal latency was recorded with an analgesia meter.For measurement of other indexes,the drug was administered at 12 days,once every 6 hours,totally five times.The measurements were performed at 14 days.Western blot assay was conducted to analyze TMEM16A expression in the L4–6 dorsal root ganglion.Immunofluorescence staining was used to detect the immunoreactivity of TMEM16A in the L4–6 dorsal root ganglion on the injured side.Patch clamp was used to detect electrophysiological changes in the neurons in the L4–6 dorsal root ganglion.Our results demonstrated that thermal withdrawal latency was shortened in the model rats compared with control rats.Additionally,TMEM16A expression and the number of TMEM16A positive cells in the L4–6 dorsal root ganglion were higher in the model rats,which induced excitation of the neurons in the L4–6 dorsal root ganglion.These findings were inhibited by T16Ainh-A01 and confirm that TMEM16A plays a key role in persistent chronic constriction injury-induced hyperalgesia.Thus,inhibiting TMEM16A might be a novel pharmacological intervention for neuropathic pain.All experimental protocols were approved by the Animal Ethics Committee at the First Affiliated Hospital of Shihezi University School of Medicine,China(approval No.A2017-170-01)on February 27,2017. 展开更多
关键词 NERVE REGENERATION TMEM16A calcium-activated chloride channels T16Ainh-A01 NEUROPATHIC pain dorsal root ganglia HYPERALGESIA action potential rheobase chronic CONSTRICTION INJURY peripheral NERVE INJURY neural REGENERATION
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Acute drivers of neuroinflammation in traumatic brain injury 预览
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作者 Kathryn L.Wofford David J.Loane D.Kacy Cullen 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1481-1489,共9页
Neuroinflammation is initiated as a result of traumatic brain injury and can exacerbate evolving tissue pathology.Immune cells respond to acute signals from damaged cells,initiate neuroinflammation,and drive the patho... Neuroinflammation is initiated as a result of traumatic brain injury and can exacerbate evolving tissue pathology.Immune cells respond to acute signals from damaged cells,initiate neuroinflammation,and drive the pathological consequences over time.Importantly,the mechanism(s)of injury,the location of the immune cells within the brain,and the animal species all contribute to immune cell behavior following traumatic brain injury.Understanding the signals that initiate neuroinflammation and the context in which they appear may be critical for understanding immune cell contributions to pathology and regeneration.Within this paper,we review a number of factors that could affect immune cell behavior acutely following traumatic brain injury. 展开更多
关键词 traumatic BRAIN INJURY inflammation NEUROINFLAMMATION MICROGLIA macrophage ACUTE diffuse BRAIN INJURY cytokines ADENOSINE 5′-triphosphoate glutamate calcium
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臂丛神经损伤规范化康复治疗的疗效分析 预览
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作者 张静 许东东 王焱 《中国卫生标准管理》 2019年第14期59-61,共3页
目的探析规范化康复治疗用于臂丛神经损伤患者中的临床效果。方法纳入2017年10月-2018年11月本院接收的92例臂丛神经损伤患者,遵从随机化原则分成参照组与试验组,参照组患者(n=46)提供常规治疗,试验组患者(n=46)提供规范化康复治疗。比... 目的探析规范化康复治疗用于臂丛神经损伤患者中的临床效果。方法纳入2017年10月-2018年11月本院接收的92例臂丛神经损伤患者,遵从随机化原则分成参照组与试验组,参照组患者(n=46)提供常规治疗,试验组患者(n=46)提供规范化康复治疗。比较两组治疗效果和臂丛神经功能。结果治疗后试验组总疗效(95.65%)高于参照组(82.61%),臂丛神经功能评分均高于参照组,差异有统计学意义(P<0.05)。结论臂丛神经损伤采用规范化康复治疗效果显著,有利于提高臂丛神经功能。 展开更多
关键词 康复治疗 治疗效果 臂丛神经 损伤 神经功能 全臂丛受损
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Protective Effects and Mechanisms of Shenhua Tablet (肾华片)on Toll-Like Receptors in Rat Model of Renal Ischemia-Reperfusion Injury
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作者 LI Qing-ping WEI Ri-bao +5 位作者 YANG Xi ZHENG Xiao-yong SU Ting-yu HUANG Meng-jie YIN Zhong CHEN Xiang-mei 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第1期37-44,共8页
Objective: To investigate the protective effects and potential mechanisms of Shenhua Tablet(肾华片, SHT) on the toll-like receptors(TLRs)-mediated signaling pathways in a rat model of kidney ischemia-reperfusion injur... Objective: To investigate the protective effects and potential mechanisms of Shenhua Tablet(肾华片, SHT) on the toll-like receptors(TLRs)-mediated signaling pathways in a rat model of kidney ischemia-reperfusion injury(IRI). Methods: Sixty male Wistar rats were randomly divided into 5 groups: sham surgery, model control, astragaloside(150 mg·kg-1·d-1), low-and high-dose SHT(1.5 and 3.0 g·kg-1·d-1, repectively) groups. One week after drug treatment, rats underwent surgery to establish the IRI models. At 24 h and 72 h after the modeling, serum creatinine(Scr) and blood urea nitrogen(BUN) were analyzed; pathological damage were scored after periodic acid-Schiff staining. TLR2, TLR4 and myeloid differentiation factor 88(My D88) protein and m RNA expressions were detected by inmmunohistochemistry, Western blot and qPCR. Tumor necrosis factor α(TNF-α) and interleukin-6(IL-6) protein expressions were detected by enzyme linked immunosorbent assay. Results: Compared with the sham group, the model group exhibited severe change in renal function(Scr: 189.42±21.50, P<0.05), pathological damage(damage score: 4.50±0.55, P<0.05), and the expression levels of TLR2, TLR4, MyD88, TNF-α, IL-6 were significantly higher than other groups. Meanwhile, the levels of TLRs in model group showed upward tendency from 24 to 72 h, unparalleled with pathological and functional changes. The aforementioned parameters were alleviated to a certain extent, and, in addition to TLRs, presented the obvious downward trending from the 24 to 72 h after the intervention in the SHT and astragaloside groups relative to the model(P<0.05); in particular, the most significant mitigation of these changes was observed in the SHT-H group(P<0.05). Conclusions: TLRs may be an important spot to treat and research in acute kidney injury. SHT could effectively mitigate renal injuries and promote recovery of IRI injuries through suppression of degeneration induced by up-regulation of TLR2 and TLR4 expression levels in the My D88-dependent signaling pathway 展开更多
关键词 SHENHUA TABLET Chinese medicine kidney INJURY TOLL-LIKE receptors MYELOID differentiation factor 88 ISCHEMIA-REPERFUSION INJURY
常山县电动自行车道路交通伤害病例特征分析 预览
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作者 刘胜 曾红霞 +2 位作者 吴正福 段家福 汪瑛 《预防医学》 2019年第7期666-668,672共4页
目的了解常山县2013—2016年电动自行车道路交通伤害病例特征,为制定预防控制措施和交通法规提供依据。方法收集常山县3家产品伤害监测哨点医院2013—2016年电动自行车道路交通伤害病例资料,采用描述流行病学方法分析人口学特征、伤害... 目的了解常山县2013—2016年电动自行车道路交通伤害病例特征,为制定预防控制措施和交通法规提供依据。方法收集常山县3家产品伤害监测哨点医院2013—2016年电动自行车道路交通伤害病例资料,采用描述流行病学方法分析人口学特征、伤害发生部位和严重程度。结果2013—2016年常山县报告电动自行车道路交通伤害病例7078例,占道路交通伤害病例的49.58%,女性电动自行车道路交通伤害占道路交通伤害的比例高于男性(P<0.05);病例以30~64岁为主,4893例占69.13%;文化程度以初中及以下为主,5562例占78.58%;电动自行车道路交通伤害主要发生在7—9月,2005例占28.33%;发生时间主要为8时—19时,5216例占73.69%;伤害部位以头部为主,2178例占30.77%;伤害严重程度以轻度为主,5306例占74.96%;伤害结局以处理后离院为主,5386例占76.09%。结论电动自行车道路交通伤害是常山县发生率较高的道路交通伤害类型,应重点提高女性和30~64岁人群安全意识,减少伤害发生。 展开更多
关键词 伤害 电动自行车 道路交通伤害
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AIS-ISS评分在法医病理学伤病关系分析中的应用
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作者 孙乐平 缪麒 +7 位作者 王殿深 张付 余彦耿 缪启锋 张曼婷 汤丽娟 赵卫东 李冬日 《中国法医学杂志》 CSCD 2019年第3期218-221,共4页
目的探讨AIS-ISS评分法在法医病理学伤病关系分析中的适用性和局限性。方法收集40例法医病理学伤病关系分析案例,对法医病理学死因分析和AIS-ISS评分结果进行比较。结果 40例案例中,AIS-ISS评分法与法医病理学死因分析评定结果相符的案... 目的探讨AIS-ISS评分法在法医病理学伤病关系分析中的适用性和局限性。方法收集40例法医病理学伤病关系分析案例,对法医病理学死因分析和AIS-ISS评分结果进行比较。结果 40例案例中,AIS-ISS评分法与法医病理学死因分析评定结果相符的案例为36例,占总数的90.0%,经双变量相关分析,得Kendall相关系数为0.872,Spearman相关系数为0.916,均为P <0.01。4例结果不相符,占总数的10.0%,其中3例为外伤后因急性肺动脉血栓栓塞或脂肪栓塞等严重并发症而死亡,法医病理学死因分析评定结果为外伤与其死亡后果之间存在因果关系,然而原发性损伤较轻;另一例其损伤程度较重,同时伴有较严重的自身疾病,法医病理学死因分析评定结果为外伤与自身疾病共同构成死亡后果,而根据AIS-ISS评分法得出外伤与死亡后果之间存在主要因果关系。结论两种评定结果具有较高的契合度,反映AIS-ISS评分法在法医病理学伤病关系分析中具有良好的应用价值,除了在外伤后因肺动脉血栓栓塞或脂肪栓塞等严重并发症致死的案例中存在局限性之外,AIS-ISS评分法在伤病关系分析中对明确外伤对死亡结果的影响程度方面更具优势,并且AIS-ISS评分法对人体不同部位和器官的损伤进行了量化和分级,其更具客观性,更容易被理解和接受。 展开更多
关键词 AIS-ISS评分法 外伤 疾病 伤病关系 法医病理学
Micromechanical adaptation as a treatment for spinal cord injury 预览
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作者 Veronica Estrada Hans Werner Muller 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1909-1911,共3页
Spinal cord injury: Thus far injury of the spinal cord is incurable and, in the majority of cases, a devastating and life-changing event. The worldwide incidence rate of spinal cord injury (SCI) ranges from 250,000 to... Spinal cord injury: Thus far injury of the spinal cord is incurable and, in the majority of cases, a devastating and life-changing event. The worldwide incidence rate of spinal cord injury (SCI) ranges from 250,000 to 900,000 (www.who.int, 2013;Kumar et al., 2018) new cases per year. SCI outcome includes the damage of axons, demyelination of axons, loss of signal transduction, and consequential long-lasting motor and sensory deficits. Additionally, the non-use of muscles can lead to atrophy and joint contractures, thereby further reducing the possibility of recovery. Depending on the spinal level and the severity of the injury, the extent of the damage can vary and spontaneous recovery is possible to varying degrees. 展开更多
关键词 MICROMECHANICAL ADAPTATION SPINAL CORD INJURY SPINAL CORD injury(SCI)
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Qian-Zheng-San promotes regeneration after sciatic nerve crush injury in rats 预览
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作者 Zhi-Yong Wang Li-Hua Qin +2 位作者 Wei-Guang Zhang Pei-Xun Zhang Bao-Guo Jiang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第4期683-691,共9页
Qian-Zheng-San,a traditional Chinese prescription consisting of Typhonii Rhizoma,Bombyx Batryticatus,Scorpio,has been found to play an active therapeutic role in central nervous system diseases.However,it is unclear w... Qian-Zheng-San,a traditional Chinese prescription consisting of Typhonii Rhizoma,Bombyx Batryticatus,Scorpio,has been found to play an active therapeutic role in central nervous system diseases.However,it is unclear whether Qian-Zheng-San has therapeutic value for peripheral nerve injury.Therefore,we used Sprague-Dawley rats to investigate this.A sciatic nerve crush injury model was induced by clamping the right sciatic nerve.Subsequently,rats in the treatment group were administered 2 mL Qian-Zheng-San(1.75 g/mL)daily as systemic therapy for 1,2,4,or 8 weeks.Rats in the control group were not administered Qian-Zheng-San.Rats in sham group did not undergo surgery and systemic therapy.Footprint analysis was used to assess nerve motor function.Electrophysiological experiments were used to detect nerve conduction function.Immunofluorescence staining was used to assess axon counts and morphological analysis.Immunohistochemical staining was used to observe myelin regeneration of the sciatic nerve and the number of motoneurons in the anterior horn of the spinal cord.At 2 and 4 weeks postoperatively,the sciatic nerve function index,nerve conduction velocity,the number of distant regenerated axons and the axon diameter of the sciatic nerve increased in the Qian-Zheng-San treatment group compared with the control group.At 2 weeks postoperatively,nerve fiber diameter,myelin thickness,and the number of motor neurons in the lumbar spinal cord anterior horn increased in the Qian-Zheng-San treatment group compared with the control group.These results indicate that Qian-Zheng-San has a positive effect on peripheral nerve regeneration. 展开更多
关键词 NERVE REGENERATION traditional Chinese medicine CRUSH INJURY peripheral NERVE REGENERATION NERVE conduction velocity SCIATIC function index NERVE INJURY NERVE repair formula SCORPION neural REGENERATION
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Effects of chondro-itinrase ABC combined with Jisuikang on neurological recovery and TGF-β1, HIF-1α and Nog-oNgR signaling pathways after spinal cord injury in rats
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作者 Yi-Ze Huang Lin Peng Ge Zhang 《海南医科大学学报(英文版)》 2019年第2期15-19,共5页
Objective:To explore the effects of chondroitinase ABC (Ch ABC) combined with spinal cord on neurological recovery and TGF-β1, HIF-1 and Nog-oNgR signaling pathways after spinal cord injury in rats.Methods: Sixty rat... Objective:To explore the effects of chondroitinase ABC (Ch ABC) combined with spinal cord on neurological recovery and TGF-β1, HIF-1 and Nog-oNgR signaling pathways after spinal cord injury in rats.Methods: Sixty rats were randomly divided into 4 groups: control group, model group, Ch ABC group and Ch ABC+ spinal cord. A rat spinal cord injury model was established using the Allen's method. Spinal nerve function was assessed by Basso Beattie Bresnahan (score) and somatosensory evoked potential (sEP) assays. mRNA levels were detected using RT-qPCR. Western blot was used to detect protein levels.Results: After modeling, the BBB scores of the rats were significantly decreased. After the intervention, the BBB scores of the three groups were improved. The BBB scores of the Ch ABC+ spinal cord group were significantly higher than those of the Ch ABC group. After modeling, the SEP of the rats was significantly increased. After the intervention, the BBB scores of the three groups were decreased. The BBB scores of the Ch ABC+ spinal cord group were significantly lower than those of the Ch ABC group. The TGF-β1 in the Ch ABC group and the Ch ABC+ spinal group was significantly higher than that in the model group, and the HIF-1 was significantly lower than the model group. The level of TGF-β1 in Ch ABC+ spinal cord group was significantly higher than that in Ch ABC group and HIF-1 was significantly lower than that in Ch ABC group. After treatment, the expression levels of Nog-oNgR pathway in Ch ABC group and Ch ABC + spinal cord group were significantly lower than those in model group, and the expression levels of Nogo-A, NgR and LINGO-1 in Ch ABC+ spinal cord group were significant after intervention. Lower than Ch ABC group.Conclusion: ChABC combined with spinal cord has a stronger role in promoting the recovery of neurological function. The combination of spinal cord can further inhibit the level of HIF-1 and increase the level of TGF-β1, and improve the prognosis to promote spinal healing. And the mechanism of actio 展开更多
关键词 SPINAL CORD INJURY SPINAL INJURY Chondro-itinrase ABC JISUIKANG
2017年北京市顺义区中小学生伤害流行现况调查
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作者 赵芳芳 赵瑞兰 +1 位作者 段军伟 李莉 《预防医学情报杂志》 CAS 2019年第1期47-50,共4页
目的了解北京市顺义区中小学生伤害流行现状,为制定干预措施提供科学依据。方法采用多阶段随机抽样方法,抽取顺义区6所中小学校学生进行问卷调查,了解过去12个月伤害发生情况和伤害相关行为发生情况。结果顺义区中小学生伤害共计调查109... 目的了解北京市顺义区中小学生伤害流行现状,为制定干预措施提供科学依据。方法采用多阶段随机抽样方法,抽取顺义区6所中小学校学生进行问卷调查,了解过去12个月伤害发生情况和伤害相关行为发生情况。结果顺义区中小学生伤害共计调查1093名学生,伤害发生率为16.83%(184/1093),其中男生为19.63%(105/535),女生为14.16%(79/558);小学生、初中生和高中生伤害发生率分别为16.39%(89/543)、19.93%(55/276)和14.60%(40/274);城区学校学生伤害发生率为22.22%(120/540),乡镇学校伤害发生率为11.57%(64/553)。伤害类型以跌倒/坠落(52.17%,96/184)、钝器伤(10.87%,20/184)、锐器伤(10.33%,19/184)为主;伤害发生地点以学校最多,占44.57%(82/184);伤害发生时前3位的活动依次为行走(28.80%,53/184)、体育活动(25.54%,47/184)和休闲娱乐(20.11%,37/184)。受伤部位以下肢和上肢为主,共占73.91%(136/184)。受伤后主要以急诊室/门诊/校医室就诊为主,占55.98%(103/184)。伤害相关行为发生率较高的主要是运动前经常不做热身和乘车经常不系安全带,分别为60.66%(663/1093)和59.19%(647/1093)。结论顺义区中小学生伤害发生率较高,伤害相关行为发生情况不容乐观,应加强社会、学校及家庭的联合干预。 展开更多
关键词 伤害 伤害相关行为 现况调查
浙江省常山县居民伤害流行状况调查
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作者 黄业伟 曾红霞 +4 位作者 刘胜 郭水梅 赵鸣 吴正福 段家福 《中国公共卫生管理》 2019年第3期371-374,共4页
目的了解浙江省常山县居民伤害流行状况及其分布特征,为制定伤害预防控制措施提供依据。方法采用多阶段整群随机抽样的方法,随机抽取常山县6个行政村共计1 200户家庭,并统一采用自制的调查问卷对2017年6月1日—2018年5月31日期间伤害发... 目的了解浙江省常山县居民伤害流行状况及其分布特征,为制定伤害预防控制措施提供依据。方法采用多阶段整群随机抽样的方法,随机抽取常山县6个行政村共计1 200户家庭,并统一采用自制的调查问卷对2017年6月1日—2018年5月31日期间伤害发生情况进行回顾性调查。结果本次共计调查2 745人,其中男性1 438人(52.39%),女性1 307人(47.61%),男女性别比为1.10∶1。浙江省常山县居民伤害发生率5.98%,其中男性高于女性(χ~2=5.406,P<0.05),死亡率15.18/10万。前3位伤害发生率分别是跌伤/坠落伤(2.05%)、交通伤(1.43%)和刀伤/锐器伤(1.29%)。各年龄段伤害发生率不同,其中以≥60岁年龄组最高(10.95%),5~15岁年龄组最低(3.51%)。受伤部位前3位分别为上肢(30.80%)、下肢(26.79%)和躯干(20.09%)。因伤害所致人均休息天数为56.02天,活动受限天数为64.43天,医疗费用为6 385.03元。结论浙江省常山县居民伤害发生率较高,因伤害所致经济和社会负担较大,应尽快制定有针对性的预防控制措施。 展开更多
关键词 居民 伤害 流行状况 发生率 流行病学
Deferoxamine promotes recovery of traumatic spinal cord injury by inhibiting ferroptosis 预览
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作者 Xue Yao Yan Zhang +12 位作者 Jian Hao Hui-Quan Duan Chen-Xi Zhao Chao Sun Bo Li Bao-You Fan Xu Wang Wen-Xiang Li Xuan-Hao Fu Yong Hu Chang Liu Xiao-Hong Kong Shi-Qing Feng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第3期532-541,共10页
Ferroptosis is an iron-dependent novel cell death pathway. Deferoxamine, a ferroptosis inhibitor, has been reported to promote spinal cord injury repair. It has yet to be clarified whether ferroptosis inhibition repre... Ferroptosis is an iron-dependent novel cell death pathway. Deferoxamine, a ferroptosis inhibitor, has been reported to promote spinal cord injury repair. It has yet to be clarified whether ferroptosis inhibition represents the mechanism of action of Deferoxamine on spinal cord injury recovery. A rat model of Deferoxamine at thoracic 10 segment was established using a modified Allen’s method. Ninety 8-week-old female Wistar rats were used. Rats in the Deferoxamine group were intraperitoneally injected with 100 mg/kg Deferoxamine 30 minutes before injury. Simultaneously, the Sham and Deferoxamine groups served as controls. Drug administration was conducted for 7 consecutive days. The results were as follows: (1) Electron microscopy revealed shrunken mitochondria in the spinal cord injury group. (2) The Basso, Beattie and Bresnahan locomotor rating score showed that recovery of the hindlimb was remarkably better in the Deferoxamine group than in the spinal cord injury group. (3) The iron concentration was lower in the Deferoxamine group than in the spinal cord injury group after injury. (4) Western blot assay revealed that, compared with the spinal cord injury group, GPX4, xCT, and glutathione expression was markedly increased in the Deferoxamine group. (5) Real-time polymerase chain reaction revealed that, compared with the Deferoxamine group, mRNA levels of ferroptosis-related genes Acyl-CoA synthetase family member 2 (ACSF2) and iron-responsive element-binding protein 2 (IREB2) were up-regulated in the Deferoxamine group. (6) Deferoxamine increased survival of neurons and inhibited gliosis. These findings confirm that Deferoxamine can repair spinal cord injury by inhibiting ferroptosis. Targeting ferroptosis is therefore a promising therapeutic approach for spinal cord injury. 展开更多
关键词 nerve REGENERATION iron SPINAL cord INJURY secondary INJURY ferroptosis DEFEROXAMINE GPX4 xCT treatment ASTROGLIOSIS lipid PEROXIDATION neural REGENERATION
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Applying the Socio-Ecological Model to barriers to implementation of ACL injury prevention programs: A systematic review
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作者 Rima L.Bogardus Ryan J.Martin +1 位作者 Alice R.Richman Anthony S.Kulas 《运动与健康科学:英文版》 SCIE 2019年第1期8-16,共9页
Background: Preventing anterior cruciate ligament(ACL) injuries is important to avoid long-term adverse health consequences. Identifying barriers to implementation of these prevention programs is crucial to reducing t... Background: Preventing anterior cruciate ligament(ACL) injuries is important to avoid long-term adverse health consequences. Identifying barriers to implementation of these prevention programs is crucial to reducing the incidence of these injuries. Our purpose was to identify barriers of implementation for ACL injury prevention programs and suggest mechanisms for reducing the barriers through application of a SocioEcological Model(SEM).Methods: Studies investigating ACL prevention program effectiveness were searched in Medline via PubMed and the Cochrane Library, and a subsequent review of the references of the identified articles, yielded 15 articles total. Inclusion criteria encompassed prospective controlled trials, published in English, with ACL injuries as the primary outcome. Studies were independently appraised by 2 reviewers for methodological quality using the PEDro scale. Barriers to implementation were identified when reported in at least 2 separate studies. A SEM was used to suggest ways to reduce the identified barriers.Results: Five barriers were identified: motivation, time requirements, skill requirements for program facilitators, compliance, and cost. The SEM suggested ways to minimize the barriers at all levels of the model from the individual through policy levels.Conclusion: Identification of barriers to program implementation and suggesting how to reduce them through the SEM is a critical first step toward enabling ACL prevention programs to be more effective and ultimately reducing the incidence of these injuries. 展开更多
关键词 Anterior CRUCIATE LIGAMENT INJURY PREVENTION Barriers to IMPLEMENTATION NEUROMUSCULAR training programs Socio-Ecological Model Sports INJURY PREVENTION
The effect of performance demands on lower extremity biomechanics during landing and cutting tasks
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作者 Boyi Dai William E.Garrett +3 位作者 Michael T.Gross Darin A.Padua Robin M.Queen Bing Yu 《运动与健康科学:英文版》 SCIE 2019年第3期228-234,共7页
Background:Anterior cruciate ligament(ACL)injuries commonly occur during the early phase of landing and cutting tasks that involve sudden decelerations.The purpose of this study was to investigate the effects of jump ... Background:Anterior cruciate ligament(ACL)injuries commonly occur during the early phase of landing and cutting tasks that involve sudden decelerations.The purpose of this study was to investigate the effects of jump height and jump speed on lower extremity biomechanics during a stop-jump task and the effect of cutting speed on lower extremity biomechanics during a side-cutting task.Methods:Thirty-six recreational athletes performed a stop-jump task under 3 conditions:jumping fast,jumping for maximum height,and jumping for 60%of maximum height.Participants also performed a side-cutting task under 2 conditions:cutting at maximum speed and cutting at 60%of maximum speed.Three-dimensional kinematic and kinetic data were collected.Results:The jumping fast condition resulted in increased peak posterior ground reaction force(PPGRF),knee extension moment at PPGRF,and knee joint stiffness and decreased knee flexion angle compared with the jumping for maximum height condition.The jumping for 60%of maximum height condition resulted in decreased knee flexion angle compared with the jumping for maxrimum height condition.Participants demonstrated greater PPGRF,knee extension moment at PPGRF,knee valgus angle and varus moment at PPGRF,knee joint stiffness,and knee flexion angle during the cutting at maximum speed condition compared with the cutting at 60%maximum speed condition.Conclusion:Performing jump landing at an increased jump speed resulted in lower extremity movement patterns that have been previously associated with an increase in ACL loading.Cutting speed also affected lower extremity biomechanics.Jump speed and cutting speed need to be considered when designing AC.L injury risk screening and injury prevention programs. 展开更多
关键词 ACL INJURY INJURY prevention KINEMATICS Kinetics Loading mechanism Risk factor
Serum and cerebrospinal fluid tau protein level as biomarkers for evaluating acute spinal cord injury severity and motor function outcome 预览
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作者 Ying Tang Hong-Liang Liu +6 位作者 Ling-Xia Min Hao-Shi Yuan Lei Guo Peng-Bo Han Yu-Xin Lu Jian-Feng Zhong Dong-Lin Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第5期896-902,共7页
Tau protein,a microtubule-associated protein,has a high specific expression in neurons and axons.Because traumatic spinal cord injury mainly affects neurons and axons,we speculated that tau protein may be a promising ... Tau protein,a microtubule-associated protein,has a high specific expression in neurons and axons.Because traumatic spinal cord injury mainly affects neurons and axons,we speculated that tau protein may be a promising biomarker to reflect the degree of spinal cord injury and prognosis of motor function.In this study,160 female Sprague-Dawley rats were randomly divided into a sham group,and mild,moderate,and severe spinal cord injury groups.A laminectomy was performed at the T8 level to expose the spinal cord in all groups.A contusion lesion was made with the NYU-MASCIS impactor by dropping a 10 g rod from heights of 12.5 mm(mild),25 mm(moderate)and 50 mm(severe)upon the exposed dorsal surface of the spinal cord.Tau protein levels were measured in serum and cerebrospinal fluid samples at 1,6,12,24 hours,3,7,14 and 28 days after operation.Locomotor function of all rats was assessed using the Basso,Beattie and Bresnahan locomotor rating scale.Tau protein concentration in the three spinal cord injury groups(both in serum and cerebrospinal fluid)rapidly increased and peaked at 12 hours after spinal cord injury.Statistically significant positive linear correlations were found between tau protein level and spinal cord injury severity in the three spinal cord injury groups,and between the tau protein level and Basso,Beattie,and Bresnahan locomotor rating scale scores.The tau protein level at 12 hours in the three spinal cord injury groups was negatively correlated with Basso,Beattie,and Bresnahan locomotor rating scale scores at 28 days(serum:r=?0.94;cerebrospinal fluid:r=?0.95).Our data suggest that tau protein levels in serum and cerebrospinal fluid might be a promising biomarker for predicting the severity and functional outcome of traumatic spinal cord injury. 展开更多
关键词 nerve REGENERATION spinal cord INJURY tau INJURY SEVERITY OUTCOME cerebrospinal fluid SERUM biomarker Basso Beattie and Bresnahan LOCOMOTOR rating scale neural REGENERATION
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