期刊文献+
共找到22,230篇文章
< 1 2 250 >
每页显示 20 50 100
MicroRNA changes of bone marrow-derived mesenchymal stem cells differentiated into neuronallike cells by Schwann cell-conditioned medium 预览
1
作者 Zhi-Jian Wei Bao-You Fan +9 位作者 Yang Liu Han Ding Hao-Shuai Tang Da-Yu Pan Jia-Xiao Shi Peng-Yuan Zheng Hong-Yu Shi Heng Wu Ang Li Shi-Qing Feng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第8期1462-1469,共8页
Bone marrow-derived mesenchymal stem cells differentiate into neurons under the induction of Schwann cells. However, key microRNAs and related pathways for differentiation remain unclear. This study screened and ident... Bone marrow-derived mesenchymal stem cells differentiate into neurons under the induction of Schwann cells. However, key microRNAs and related pathways for differentiation remain unclear. This study screened and identified differentially expressed microRNAs in bone marrow- derived mesenchymal stem cells induced by Schwann cell-conditioned medium, and explored targets and related pathways involved in their differentiation into neuronal-like cells. Primary bone marrow-derived mesenchymal stem cells were isolated from femoral and tibial bones, while primary Schwann cells were isolated from bilateral saphenous nerves. Bone marrow-derived mesenchymal stem cells were cultured in unconditioned (control group) and Schwann cell-conditioned medium (bone marrow-derived mesenchymal stem cell + Schwann cell group). Neuronal differentiation of bone marrow-derived mesenchymal stem cells induced by Schwann cell-conditioned medium was observed by time-lapse imaging. Upon induction, the morphology of bone marrow-derived mesencaymal stem cells changed into a neural shape with neurites. Results of quantitative reverse transcription-polymerase chain reaction revealed that nestin mRNA expression was upregulated from 1 to 3 days and downregulated from 3 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group. Compared with the control group, microtubule-associated protein 2 mRNA expression gradually increased from 1 to 7 days in the bone marrow-derived mesenchymal stem cell + Schwann cell group. After 7 days of induction, microRNA analysis iden:ified 83 significantly differentially expressed microRNAs between the two groups. Gene Ontology analysis indicated enrichment of microRNA target genes for neuronal projection development, regulation of axonogenesis, and positive regulation of cell proliferation. Kyoto Encyclopedia of Genes and Genomes pathway analysis demonstrated that Hippo, Wnt, transforming growth factor-beta, and Hedgehog signaling pathv/ays were potentially associated with neural differentiation of b 展开更多
关键词 nerve REGENERATION MICRORNA analysis bone marrow-derived mesenchymal stem cells: Schwann CELLS neuronal-like CELLS neuronal differentiation Gene Ontology analysis Hippo SIGNALING PATHWAY Wnt SIGNALING PATHWAY transforming growth FACTOR-BETA SIGNALING PATHWAY Hedgehog SIGNALING PATHWAY neural REGENERATION
在线阅读 下载PDF
脊髓损伤后肌肉萎缩基因谱的生物信息学分析 预览
2
作者 黄晖 王广积 《中国组织工程研究》 CAS 北大核心 2019年第27期4269-4274,共6页
背景:脊髓损伤后常伴随肌肉萎缩的发生,但是它的基本机制仍然不是十分清楚。目的:旨在探讨脊髓损伤后肌肉萎缩的分子生物学机制。方法:分析基因表达数据库中的脊髓损伤后肌肉萎缩的基因谱GSE45550。基因表达谱GSE45550包括对照组(脊髓... 背景:脊髓损伤后常伴随肌肉萎缩的发生,但是它的基本机制仍然不是十分清楚。目的:旨在探讨脊髓损伤后肌肉萎缩的分子生物学机制。方法:分析基因表达数据库中的脊髓损伤后肌肉萎缩的基因谱GSE45550。基因表达谱GSE45550包括对照组(脊髓损伤前)、实验组1(脊髓损伤后3 d)、实验组2(脊髓损伤后8 d)、实验组3(脊髓损伤后14 d)。组织为SD大鼠的比目鱼肌,每组6例。随后对4组样本数据进行差异基因分析、GO分析、通路分析。结果与结论:确定了2 513个差异表达基因,其中Wnt16、Obfc1、Ufd1l、LOC100361067、Hhatl、Fxyd1、Psmc4、Tasp1、Mettl21c、Ufd1l差异表达最显著。GO分析显示差异基因的主要生物学过程为biological_process、G蛋白偶联受体信号通路、对药物的反应、DNA依赖性转录、DNA依赖性转录的正调节、氧化还原过程、泛素依赖性蛋白分解代谢过程、凋亡过程、RNA聚合酶转录的正调控及脂肪酸β-氧化。信号通路如MAPK信号、细胞凋亡、柠檬酸循环(TCA循环)可能起到重要的作用。研究比较完整地揭示了脊髓损伤后肌肉萎缩基因谱的差异表达基因和所涉及的生物学过程和信号通路,其中Wnt16可能是脊髓损伤后肌肉萎缩中的关键基因,为未来的治疗进展提供分子靶点。 展开更多
关键词 脊髓损伤 肌肉萎缩 通路 基因 生物学过程 差异基因分析 GO分析 通路分析
在线阅读 下载PDF
Estrogen and insulin synergistically promote endometrial cancer progression via crosstalk between their receptor signaling pathways 预览
3
作者 Wenyan Tian Fei Teng +7 位作者 Jinping Gao Chao Gao Guoyan Liu Yanfang Zhang Shizhu Yu Wei Zhang Yingmei Wang Fengxia Xue 《癌症生物学与医学:英文版》 CAS CSCD 2019年第1期55-65,共11页
Objective: Despite evidence that estrogens and insulin are involved in the development and progression of many cancers, their synergistic role in endometrial carcinoma(EC) has not been analyzed yet.Methods: Here, we i... Objective: Despite evidence that estrogens and insulin are involved in the development and progression of many cancers, their synergistic role in endometrial carcinoma(EC) has not been analyzed yet.Methods: Here, we investigated how estrogens act synergistically with insulin to promote EC progression. Cell growth in vitro and in vivo, effects of estradiol and insulin on apoptosis and cell cycle distribution, and expression and activation of estrogen receptor(ER), insulin receptor(InsR), and key proteins in the PI3K and MAPK pathways were examined after combined stimulation with estradiol and insulin.Results: Compared to EC cells treated with estradiol or insulin alone, those treated with both estradiol and insulin exhibited stronger stimulation. Estradiol significantly induced phosphorylation of InsR-β and IRS-1, whereas insulin significantly induced phosphorylation of ER-α. In addition, treatment with both insulin and estradiol together significantly increased the expression and phosphorylation of Akt, MAPK, and ERK. Notably, InsR-β inhibition had a limited effect on estradiol-dependent proliferation,cell cycle, and apoptosis, whereas ER-α inhibition had a limited insulin-dependent effect, in EC cell lines. Insulin and estradiol individually and synergistically promoted EC xenograft growth in mice.Conclusions: Estrogen and insulin play synergistic roles in EC carcinogenesis and progression by activating InsR-β and ER-α,promoting a crosstalk between them, and thereby resulting in the activation of downstream PI3K/Akt and MAPK/ERK signaling pathways. 展开更多
关键词 ENDOMETRIAL cancer(EC) ESTROGEN INSULIN InsR-β ER-α PI3K/Akt PATHWAY MAPK/ERK PATHWAY
在线阅读 下载PDF
Patrinia scabiosaefolia Inhibits Growth of 5-FU-Resistant Colorectal Carcinoma Cells via Induction of Apoptosis and Suppression of AKT Pathway
4
作者 HUANG Si-zhou LIU Wang-yu +3 位作者 HUANG Yue SHEN A-ling LIU Li-ya PENG Jun 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第2期116-121,共6页
Objective: To investigate the effects of ethanol extract of Patrinia scabiosaefolia(EEPS) on chemo-resistance of colorectal cancer cells(CRC) and explore the possible molecular mechanisms. Methods: 5-fluorouracil(5-FU... Objective: To investigate the effects of ethanol extract of Patrinia scabiosaefolia(EEPS) on chemo-resistance of colorectal cancer cells(CRC) and explore the possible molecular mechanisms. Methods: 5-fluorouracil(5-FU)-resistant human colorectal carcinoma cell line(HCT-8/5-FU) and its parental cells HCT-8 were treated with EEPS(0, 0.25, 0.50, 1 or 2 mg/mL), or 5-FU(0, 100, 200, 400, 800 or 1600 μmol/L). The 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide(MTT) assay was performed to evaluate the cell viability. Cell density was observed by phase-contrast microscope, cell counting and colony formation assay were used to determine the cell proliferation of HCT-8/5-FU cells treated with 0, 0.5, 1 or 2 mg/mL EEPS. Cell apoptosis was determined by Hoechst staining. Western-blot was performed to detect the phosphorylation of AKT as well as the protein expression level of B-cell CLL/lymphoma 2(Bcl-2) and Bcl-2-associated X protein(Bax). Results: Compared with HCT-8 cells, MTT assay results indicated that HCT-8/5-FU cells were resistant to 5-FU treatment(P<0.05), and sensitive to EEPS treatment(P>0.05). Moreover, compared with untreated HCT-8/5-FU cells, 1 and 2 mg/mL of EEPS treatment significantly reduced cell density, cell number, inhibited cell survival(P<0.05), and induced apoptosis in HCT-8/5-FU cells. Furthermore, 1 and 2 mg/mL of EEPS significantly decreased the phosphorylation level of p-AKT and Bcl-2 protein expression, and increased the expression of Bax protein(P<0.05). Conclusion: EEPS is a promising therapeutic agent that may overcome chemo-resistance in cancer cells, likely through suppression of the AKT pathway and promotion of cancer cell apoptosis. 展开更多
关键词 AKT PATHWAY COLORECTAL cancer 5-FLUOROURACIL resistance Patrinia scabiosaefolia Chinese medicine
Bioinformatics Based Therapeutic Effects of Sinomenium Acutum
5
作者 LI Yu-yan ZHENG Guang LIU Liang 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第2期122-130,共9页
Objective: To decipher the possible mechanisms of Sinomenium Acutum(SA) in treating diseases by a bioinformatics method. Methods: SA ingredients were searched according to Chinese Pharmacopoeia, Chinese Medicine Dicti... Objective: To decipher the possible mechanisms of Sinomenium Acutum(SA) in treating diseases by a bioinformatics method. Methods: SA ingredients were searched according to Chinese Pharmacopoeia, Chinese Medicine Dictionary and Traditional Chinese Medicines Database(TCMD). Active compounds and target proteins of SA were acquired through the Pubchem platform. Pathway, network and function analyses of SA were performed with ingenuity pathway analysis(IPA), a bioinformatics analysis platform. Disease, biofunction-target networks were established with Cytoscape. Results: Eighteen ingredients from SA were obtained. Seven active ingredients with 31 active target proteins were acquired according to PubChem Bioassay test. By IPA analysis, 277 canonical pathways belonging to 17 function categories were collected, 23 kinds of diseases, 21 categories bio-functions were obtained. Based on P value, calculated by IPA, the top 5 significant pathway of SA targets include phosphatidylinositol 3 kinase/Akt(PI3 K/Akt) signaling, prostate cancer signaling, macrophage migration inhibitory factor(MIF) regulation of innate immunity, Guanosine-binding protein coupled receptor(GPCR) signaling, and ataxia telangiectasia mutated protein(ATM) signaling. Disease and bio-function network analysis indicated that mitogen activated protein kinase 1(MAPK1), MAPK3, p65 nuclear factor κB(RELA), nuclear factor of κB inhibitor alpha(NFκBIA), interleukin 1β(IL-1β), prostaglandin G/H synthase 2(PTGS2) and tumor protein 53(TP53) were the critical targets in various diseases treated by SA. Conclusions: In the different view of target, pathway, disease and bio-function, inflammation was found to be a central theme in many chronic conditions. SA could be used not only as an anti-inflammatory agent, but also for the treatment of cancers, neurological diseases, psychological disorders and metabolic diseases. 展开更多
关键词 Sinomenium Acutum BIOINFORMATICS ANALYSIS INGENUITY PATHWAY ANALYSIS NETWORK PHARMACOLOGY
Atsttrin reduces lipopolysaccharide-induced neuroinflammation by inhibiting the nuclear factor kappa B signaling pathway 预览
6
作者 Lian Liu Yuan Qu +7 位作者 Yi Liu Hua Zhao He-Cheng Ma Ahmed Fayyaz Noor Chang-Jiao Ji Lin Nie Meng Si Lei Cheng 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1994-2002,共9页
Progranulin is closely related to neuronal survival in a neuroinflammatory mouse model and attenuates inflammatory reactions. Atsttrin is an engineered protein composed of three progranulin fragments and has been show... Progranulin is closely related to neuronal survival in a neuroinflammatory mouse model and attenuates inflammatory reactions. Atsttrin is an engineered protein composed of three progranulin fragments and has been shown to have an effect similar to that of progranulin. Atsttrin has anti-inflammatory actions in multiple arthritis mouse models, and it protects against further arthritis development. However, whether Atsttrin has a role in neuroinflammation remains to be elucidated. In this study, we produced a neuroinflammatory mouse model by intracerebroventricular injection of 1 μL lipopolysaccharide(10 μg/μL). Atsttrin(2.5 mg/kg) was administered via intraperitoneal injection every 3 days over a period of 7 days before intracerebroventricular injection of 1 μL lipopolysaccharide(10 μg/μL). In addition, astrocyte cultures were treated with 0, 100 or 300 ng/mL lipopolysaccharide, with 200 ng/mL Atsttrin simultaneously. Immunohistochemistry, enzyme-linked immunosorbent assay and real-time reverse transcription-polymerase chain reaction were performed to examine the protein and mRNA levels of inflammatory mediators and to assess activation of the nuclear factor kappa B signaling pathway. Progranulin expression in the brain of wild-type mice and in astrocyte cultures was increased after lipopolysaccharide administration. The protein and mRNA expression levels of tumor necrosis factor-α, interleukin-1β and inducible nitric oxide synthase were increased in the brain of progranulin knockout mice after lipopolysaccharide administration. Atsttrin treatment reduced the lipopolysaccharide-induced increase in the protein and mRNA levels of tumor necrosis factor-α, interleukin-1β, matrix metalloproteinase-3 and inducible nitric oxide synthase in the brain of progranulin knockout mice. Atsttrin also reduced the expression of cyclooxygenase-2, inducible nitric oxide synthase and matrix metalloproteinase 3 mRNA in lipopolysaccharide-treated astrocytes in vitro, and decreased the concentration of tumor necrosis factor α 展开更多
关键词 nerve REGENERATION progranulin Atsttrin NEUROINFLAMMATION inflammatory cytokines LIPOPOLYSACCHARIDE INTRACEREBROVENTRICULAR injection ASTROCYTE nuclear factor kappa B signaling pathway progranulin knockout mouse CEREBROSPINAL fluid neural REGENERATION
在线阅读 下载PDF
水通道蛋白AQP4对乳腺癌细胞增殖的影响及机制 预览
7
作者 李英彬 孙圣荣 《现代肿瘤医学》 CAS 2019年第6期943-946,共4页
目的:研究AQP4在人乳腺癌细胞中的表达,并进一步探讨AQP4对肿瘤细胞增殖的影响及机制。方法:通过siRNA干扰技术下调乳腺癌细胞T47D和MCF-7细胞系中水通道蛋白4表达,检测这种下调对乳腺癌细胞增殖的影响并初步探讨其作用机制。结果:AQP4... 目的:研究AQP4在人乳腺癌细胞中的表达,并进一步探讨AQP4对肿瘤细胞增殖的影响及机制。方法:通过siRNA干扰技术下调乳腺癌细胞T47D和MCF-7细胞系中水通道蛋白4表达,检测这种下调对乳腺癌细胞增殖的影响并初步探讨其作用机制。结果:AQP4表达下降抑制乳腺癌细胞的增殖,可能通过ERK通路/E-钙黏蛋白实现的。结论:AQP4表达水平与乳腺癌细胞增殖正相关,相应的肿瘤抑制剂可以起到控制肿瘤生长作用。 展开更多
关键词 水通道蛋白4 小干扰RNA 增殖 通路
在线阅读 下载PDF
Pathways of Influence of the Northern Hemisphere Mid–high Latitudes on East Asian Climate:A Review 预览
8
作者 Jianping LI Fei ZHENG +2 位作者 Cheng SUN Juan FENG Jing WANG 《大气科学进展:英文版》 SCIE CAS CSCD 2019年第9期902-921,共20页
This paper reviews recent progress made by Chinese scientists on the pathways of influence of the Northern Hemisphere mid-high latitudes on East Asian climate within the framework of a“coupled oceanic-atmospheric(lan... This paper reviews recent progress made by Chinese scientists on the pathways of influence of the Northern Hemisphere mid-high latitudes on East Asian climate within the framework of a“coupled oceanic-atmospheric(land-atmospheric or seaice-atmospheric)bridge”and“chain coupled bridge”.Four major categories of pathways are concentrated upon,as follows:Pathway A—from North Atlantic to East Asia;Pathway B—from the North Pacific to East Asia;Pathway C—from the Arctic to East Asia;and Pathway D—the synergistic effects of the mid-high latitudes and tropics.In addition,definitions of the terms“combined effect”,“synergistic effect”and“antagonistic effect”of two or more factors of influence or processes and their criteria are introduced,so as to objectively investigate those effects in future research. 展开更多
关键词 East Asian climate Northern HEMISPHERE mid-high LATITUDES COUPLED oceanic-land-sea-ice-atmospheric BRIDGE chain COUPLED BRIDGE pathway synergistic effect
在线阅读 下载PDF
Attenuation of lipopolysaccharide-induced neuroinflammatory events in BV-2 microglial cells by Moringa oleifera leaf extract
9
作者 Gothai Sivaprakasam Palanivel Ganesan +5 位作者 Katyakyini Muniandy Shin-Young Park Duk-Yeon Cho Joon-So Kim Palanisamy Arulselvan Dong-Kug Choi 《亚太热带生物医学杂志:英文版》 CAS 2019年第3期109-115,共7页
Objective: To determine the anti-neuroinflammatory activity of Moringa oleifera leaf extract(MLE) under lipopolysaccharide stimulation of mouse murine microglia BV2 cells in vitro. Methods: The cytotoxicity effect of ... Objective: To determine the anti-neuroinflammatory activity of Moringa oleifera leaf extract(MLE) under lipopolysaccharide stimulation of mouse murine microglia BV2 cells in vitro. Methods: The cytotoxicity effect of MLE was investigated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide assay. The inflammatory response of BV-2 cells were induced with lipopolysaccharide. The generation of nitric oxide levels was determined by using Griess assay and the level of pro-inflammatory cytokines(IL-1β, IL-6 and TNF-α) was evaluated by ELISA kit. The expression of iNOS, COX-2 as well as IκB-ααwas carried out by immunoblot analysis. Results: MLE reduced the nitric oxide production in concentration-dependent manner, and maintained the viability of BV-2 microglial cells which indicated absence of toxicity. In addition, MLE repressed the activation of nuclear factor kappa B by arresting the deterioration of IκB-α, consequently resulted in suppression of cytokines expression such as COX-2 and iNOS. Conclusions: MLE inhibitory activities are associated with the inhibition of nuclear factor kappa B transcriptional activity in BV2 microglial cells. Thus MLE may offer a substantial treatment for neuroinflammatory diseases. 展开更多
关键词 Moringa oleifera leaf extract BV2 MICROGLIAL cells Neuro-inflammation PRO-INFLAMMATORY CYTOKINES NF-κB signaling pathway
Effect of crystalline structure on terbuthylazine degradation by H2O2-assisted TiO2 photocatalysis under visible irradiation
10
作者 Jianjun Tang Yiqing Chen Zijun Dong 《环境科学学报:英文版》 SCIE EI CAS CSCD 2019年第5期153-160,共8页
Various methods for shifting the optical response of TiO2 into the visible(Vis) range have been reported. Herein, we reported the application of a TiO2/H2O2/Vis process and the effects of TiO2 crystalline structure on... Various methods for shifting the optical response of TiO2 into the visible(Vis) range have been reported. Herein, we reported the application of a TiO2/H2O2/Vis process and the effects of TiO2 crystalline structure on the degradation of terbuthylazine. The results indicated that TiO2 crystalline structure and H2O2 addition had significant effects on terbuthylazine degradation: its degradation rate could be increased from 7% to 70% with H2O2 addition after 180 min of reaction, the synergistic degradation of terbuthylazine by TiO2-Fe3+ was substantially accelerated, with the degradation rate reaching up to 100% after20 min of reaction, and rutile TiO2 showed better photocatalytic activity and a more obvious synergistic effect than anatase TiO2. The addition of free-radical scavengers(tert-butyl alcohol or methanol) inhibited the degradation efficiency of rutile TiO2, but had a relatively minor effect on anatase TiO2. Fluorescence spectrophotometry analysis indicated that hydroxyl free radicals could be continuously produced when using rutile TiO2 as the photocatalyst. Degradation of terbuthylazine catalyzed by rutile TiO2 occurred mainly in solution, but occurred on the particle surface of the photocatalyst when catalyzed by anatase TiO2. This study provides new insight into the role of TiO2 crystalline structure on the degradation of terbuthylazine and its photocatalytic degradation mechanism. 展开更多
关键词 VISIBLE-LIGHT PHOTOCATALYSIS Reaction pathway HYDROXYL free RADICAL Hydrogen PEROXIDE
Wnt signaling pathways in myocardial infarction and the therapeutic effects of Wnt pathway inhibitors
11
作者 Wen-bin Fu Wei Eric Wang Chun-yu Zeng 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第1期9-12,共4页
Myocardial infarction (MI)is one of the most serious health threats,resulting in huge physical and economic burdens worldwide. Wnt signaling pathways play an important role in developmental processes such as tissue pa... Myocardial infarction (MI)is one of the most serious health threats,resulting in huge physical and economic burdens worldwide. Wnt signaling pathways play an important role in developmental processes such as tissue patterning,cell differentiation and cell division.Appropriate regulation of the activities of Wnt signaling pathways is also important for heart development and healing in post-MI heart.Moreover,Wnt pathway inhibitors have been identified as novel antitumor drugs and applied in ongoing clinical trials.This research progress has generated increasing interests for investigating the effects of Wnt pathway inhibitors on MI healing.In this short review,we summarize the roles of Wnt signaling pathways in post-MI heart and the therapeutic effects of Wnt pathway inhibitors on MI,and discuss the underlying mechanisms of Wnt pathway inhibitors in cardiac repairing. 展开更多
关键词 Wnt pathway INHIBITORS MYOCARDIAL INFARCTION CARDIAC repairing THERAPEUTIC effect
The aldose reductase inhibitor epalrestat exerts nephritic protection on diabetic nephropathy in db/db mice through metabolic modulation
12
作者 Jun He Hao-xue Gao +9 位作者 Na Yang Xiao-dong Zhu Run-bin Sun Yuan Xie Cai-hong Zeng Jing-wei Zhang Jian-kun Wang Fei Ding Ji-ye Aa Guang-ji Wang 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第1期86-97,共12页
Epalrestat is an inhibitor of aldose reductase in the polyol pathway and is used for the management of diabetic neuropathy clinically.Our pilot experiments and accumulated evidences showed that epalrestat inhibited po... Epalrestat is an inhibitor of aldose reductase in the polyol pathway and is used for the management of diabetic neuropathy clinically.Our pilot experiments and accumulated evidences showed that epalrestat inhibited polyol pathway and reduced sorbitol production,and suggested the potential renal protection effects of epalrestat on diabetic nephropathy (DN).To evaluate the protective effect of epalrestat,the db/db mice were used and exposed to epalrestat for 8 weeks,both the physiopathological condition and function of kidney were examined.For the first time,we showed that epalrestatmarkedlyreduced albuminuria and alleviated the podocyte foot process fusion and interstitial fibrosis of db/db mice.Metabolomics was employed,and metabolites in the plasma,renal cortex,and urine were profiled using a gas chromatography-mass spectrometry (GC/MS)-based metabolomic platform.We observed an elevation of sorbitol and fructose,and a decrease of myo-inositol in the renal cortex of db/db mice. Epalrestat reversed the renal accumulation of the polyol pathway metabolites of sorbitol and fructose,and increased myo-inositol level.Moreover,the upregulation of aldose reductase,fibronectin,collagen Ⅲ,and TGF-β1 in renal cortex of db/db mice was downregulated by epalrestat.The data suggested that epalrestat has protective effects on DN,and the inhibition ofaldose reductas and the modulation of polyol pathway in nephritic cells be a potentially therapeutic strategy for DN. 展开更多
关键词 diabetes DIABETIC NEPHROPATHY POLYOL pathway metabolomics ALDOSE REDUCTASE
Long noncoding RNA LINC00520 prevents the progression of cutaneous squamous cell carcinoma through the inactivation of the PI3K/Akt signaling pathway by downregulating EGFR
13
作者 Xue-Ling Mei Shah Zhong 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第4期454-465,共12页
Background: Long noncoding RNAs (lncRNAs) play pivotal roles in various malignant tumors. Epidermal growth factor receptor (EGFR) signaling is associated with the pathogenesis of cutaneous squamous cell carcinoma (cSC... Background: Long noncoding RNAs (lncRNAs) play pivotal roles in various malignant tumors. Epidermal growth factor receptor (EGFR) signaling is associated with the pathogenesis of cutaneous squamous cell carcinoma (cSCC). This study aimed to explore the role of LINC00520 in the development of cSCC via EGFR and phosphoinositide 3-kinase-protein kinase B (PI3K/Akt) signaling pathways. Methods: A microarray analysis was applied to screen differentially expressed lncRNAs in cSCC samples. The A431 cSCC cell line was transfected and assigned different groups. The expression patterns of LINC00520, EGFR, and intermediates in the PI3K/Akt pathway were characterized using reverse transcription quantitative polymerase chain reaction (RT-qPCR) and Western blotting analysis. Cell proliferation, migration, and invasion were detected using the MTT assay, scratch test, and Transwell assay, respectively. Cell-based experiments and a tumorigenicity assay were conducted to assess the effect of LINC00520 on cSCC progression. This study was ended in September 2017. Comparisons between two groups were analyzed with t-test and comparisons among multiple groups were analyzed using one-way analysis of variance. The nonparametric Wilcoxon rank sum test was used to analyze skewed data. The enumerated data were analyzed using the chi-square test or Fisher exact test. Results: Data from chip GSE66359 revealed depletion of LINC00520 in cSCC. Cells transfected with LINC00520 vector and LINC00520 vector + si-EGFR showed elevated LINC00520 level but decreased levels of the EGFR, PI3K, AKT, VEGF, MMP-2 and MMP-9 mRNAs and proteins, and inhibition of the growth, migration and adhesion of cSCC cells, while the si-LINC00520 group showed opposite trends (all P < 0.05). Compared with the LINC00520 vector group, the LINC00520 vector + si-EGFR group showed decreased levels of the EGFR, PI3K, AKT, VEGF, MMP-2 and MMP-9 mRNAs and proteins, and inhibition of the growth, migration and adhesion of cSCC cells, while the LINC00520 vector+ EGFR vector group show 展开更多
关键词 LINC00520 EGFR PI3K/AKT signaling pathway CUTANEOUS SQUAMOUS cell carcinoma LYMPHATIC vessel INVASION INVASION Metastasis
Revelation of the birth and extinction dynamics of solitons in SWNT-mode-locked fiber lasers
14
作者 Yudong Cui Xueming Liu 《光子学研究:英文版》 CSCD 2019年第4期423-430,共8页
The dispersive Fourier transform(DFT) technique opens a fascinating pathway to explore ultrafast non-repetitive events and has been employed to study the build-up process of mode-locked lasers. However, the shutting p... The dispersive Fourier transform(DFT) technique opens a fascinating pathway to explore ultrafast non-repetitive events and has been employed to study the build-up process of mode-locked lasers. However, the shutting process for the mode-locked fiber laser seems to be beyond the scope of researchers, and the starting dynamics under nearzero dispersion remains unclear. Here, the complete evolution dynamics(from birth to extinction) of the conventional soliton(CS), stretched pulse(SP), and dissipative soliton(DS) are investigated by using the DFT technique.CS, SP, and DS fiber lasers mode locked by single-walled carbon nanotubes(SWNTs) are implemented via engineering the intracavity dispersion map. The relaxation oscillation can always be observed before the formation of stable pulse operation due to the inherent advantage of SWNT, but it exhibits distinct evolution dynamics in the starting and shutting processes. The shutting processes are dependent on the dispersion condition and turn-off time, which is against common sense. Some critical phenomena are also observed, including transient complex spectrum broadening and frequency-shift interaction of SPs and picosecond pulses. These results will further deepen understanding of the mode-locked fiber laser from a real-time point of view and are helpful for laser design and applications. 展开更多
关键词 dispersive Fourier transform(DFT) fascinating pathway PICOSECOND pulses
Inhibitory effects of petasin on human colon carcinoma cells mediated by inactivation of Akt/mTOR pathway
15
作者 Xi Lyu Ai-Lin Song +3 位作者 Yin-Liang Bai Xiao-Dong Xu Dong-Qiang He You-Cheng Zhang 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第9期1071-1078,共8页
Background:Colorectal cancer is the third most common cancer worldwide and still lack of effective therapy so far.Petasin,a natural product found in plants of the genus Petasites,has been reported to possess anticance... Background:Colorectal cancer is the third most common cancer worldwide and still lack of effective therapy so far.Petasin,a natural product found in plants of the genus Petasites,has been reported to possess anticancer activity.The present study aimed to investigate the anticolon cancer activity of petasin both in vitro and in vivo.The molecular mechanism of petasin was also further explored.Methods:Caco-2,LoVo,SW-620,and HT-29 cell lines were used to detect the inhibitory effect of petasin on colon cancer proliferation.Cell viability was determined using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay.Cell apoptosis was analyzed by flow cytometry.Hoechst 33258 staining was used to visualize morphological changes.Cell migration was assessed using a wound-healing migration assay,and cell invasion was investigated using Transwell chambers.Western blotting assays were employed to evaluate the expression levels of proteins in the protein kinase B/mammalian target of rapamycin (Akt/mTOR) signaling pathway.Finally,in vivo activity of petasin was evaluated using the SW-620 subcutaneous tumor model established in Balb/c nude mice.Twelve rats were randomly divided into control group and 10 mg/kg petasin group.The tumor volume was calculated every 7 days for 28 days.Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay was performed to assess the apoptotic effect of petasin.Differences between two groups were assessed by analysis of independent-sample t tests.Results:Petasin significantly inhibited the proliferation of human colon carcinoma cell lines,induced apoptosis,and suppressed migration and invasion in SW-620 cells.Western blotting results showed that petasin decreased the phosphorylation of Akt (1.01 ± 0.16 vs.0.74 ± 0.06,P = 0.042),mTOR (0.71 ± 0.12 vs.0.32 ± 0.11,P = 0.013),and P70S6K (1.23 ± 0.21 vs.0.85 ± 0.14,P = 0.008),elevated the expression of caspase-3 (0.41 ± 0.09 vs.0.74 ± 0.12,P = 0.018) and caspase-9 (1.10 ± 0.27 vs.1.98 ± 0.22,P = 0. 展开更多
关键词 Petasin COLON cancer Apoptosis MIGRATION INVASION AKT/MTOR PATHWAY
Umbelliferone ameliorates renal function in diabetic nephropathy rats through regulating inflammation and TLR/NF-κB pathway
16
作者 WANG Han-Qing WANG Sha-Sha +2 位作者 Chiufai Kuok WANG Qi CHENG Xiao-Lan 《中国天然药物:英文版》 SCIE CAS CSCD 2019年第5期346-354,共9页
Diabetic nephropathy (DN) is a leading cause of renal failure, contributing to severe morbidity and mortality in diabetic patients. Umbelliferae (Umb) has been well characterized to exert protective effects in diabete... Diabetic nephropathy (DN) is a leading cause of renal failure, contributing to severe morbidity and mortality in diabetic patients. Umbelliferae (Umb) has been well characterized to exert protective effects in diabetes. However, the action and mechanism of Umb in DN remains unclear. In this work, we studied the effect of Umb in a streptozotocin (STZ)-induced DN rat model and explore its underlying mechanism. DN rats were treated with Umb (20, 40 mg·kg^-1) or irbesartan (15 mg·kg^-1) for 4 weeks. Levels of serum glucose, insulin, blood uric acid, creatinine, triglycerides (TG) and total cholesterol (TC) were measured by commercial assay kits, respectively. Histopathological changes and inflammatory cytokine levels including IL-6, IL-1β and TNF-α in the kidney were also evaluated. Alterations in the expression of podocin, CD2AP and TLR/NF-κB were assessed by western blotting. Our results showed that Umb reduced renal injury in DN rat model, as evidenced by the decrease in blood glucose, 24 h urinary protein, serum creatinine, and blood uric acid. Umb also significantly ameliorated the renal histopathological alteration, and down-regulated the expression of epithclial-to-mesenchymal transition-related molecular markers podocin and CD2AP. Moreover, Umb inhibited TLR2, TLR4, MyD88 expressions, NF-κB activation and considerably reduced levels of other downstream inflammatory molecules (TNF-α, IL-6, IL-1β). These findings indicated that Umb improved renal function through regulating inflammation and TLR/NF-κB pathway, suggesting the potential efficacy of Umb in DN treatment. 展开更多
关键词 DIABETIC NEPHROPATHY Umbelliferone STREPTOZOTOCIN INFLAMMATION TLR/NF-kB pathway
Effect of Soothing Gan(Liver) and Invigorating Pi(Spleen) Recipes on TLR4-p38 MAPK Pathway in Kupffer Cells of Non-alcoholic Steatohepatitis Rats
17
作者 GONG Xiang-wen XU Yong-jian +4 位作者 YANG Qin-he LIANG Yin-ji ZHANG Yu-pei WANG Guan-long LI Yuan-yuan 《中国结合医学杂志:英文版》 SCIE CAS CSCD 2019年第3期216-224,共9页
Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and ... Objective: To investigate the mechanism of inflammatory-mediated toll-like receptor 4(TLR4)-p38 mitogen-activated protein kinase(p38 MAPK) pathway in Kupffer cells(KCs) of non-alcoholic steatohepatitis(NASH) rats and the intervention effect of soothing Gan(Liver) and invigorating Pi(Spleen) recipes on this pathway. Methods: After 1 week of acclimatization, 120 Sprague-Dawley male rats were randomly divided into 8 groups using a random number table(n=15 per group): normal group, model group, low-dose Chaihu Shugan Powder(柴胡疏肝散, CHSG) group(3.2 g/kg), high-dose CHSG group(9.6 g/kg), low-dose Shenling Baizhu Powder(参苓白术散, SLBZ) group(10 g/kg), high-dose SLBZ(30 g/kg) group, and low-and highdose integrated recipe(L-IR, H-IR) groups. All rats in the model and treatment groups were fed with a high-fat diet(HFD). The treatments were administrated by gastrogavage once daily and lasted for 26 weeks. The liver tissues were detected with hematoxylin-eosin(HE) and oil red O staining. Levels of liver lipids, serum lipids and transaminases were measured. KCs were isolated from the livers of rats to evaluate the mRNA expressions of TLR4 and p38 MAPK by real-time fluorescence quantitative polymerase chain reaction, and proteins expressions of TLR4, p-p38 MAPK and p38 MAPK by Western blot. Levels of inflammatory cytokines including tumor necrosis factor α(TNF-α), interleukin(IL)-1 and IL-6 in KCs were measured by enzyme-linked immunosorbent assay. Results: After 26 weeks of HFD feeding, HE and oil red O staining showed that the NASH model rats successfully reproduced typical pathogenesis and histopathological features. Compared with the normal group, the model group exhibited significant increases in body weight, liver weight, liver index, serum levels of total cholesterol(TC), triglyceride(TG), low-density lipoprotein cholesterol, and aspartate aminotransferase as well as TC and TG levels in liver tissues, and significant decrease in serum level of high-density lipoprotein cholesterol(P<0.05 or P<0.01), whi 展开更多
关键词 non-alcoholic steatohepatitis soothing GAN (Liver)and invigorating PI (Spleen)recipes Kupffer cel toll-like receptor 4-p38 MITOGEN-ACTIVATED protein kinase signaling pathway inflammation Chinese medicine
Naloxone attenuates ischemic brain injury in rats through suppressing the NIK/IKKα/NF-κB and neuronal apoptotic pathways
18
作者 Xuan Wang Zu-jun Sun +5 位作者 Jun-lu Wu Wen-qiang Quan Wei-dong Xiao Helen Chew Cui-min Jiang Dong Li 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第2期170-179,共10页
Although naloxone has been documented to exert neuroprotection in animal model of cerebral ischemia, the mechanism is not well understood. In this present study we investigated whether naloxone affected the mitochondr... Although naloxone has been documented to exert neuroprotection in animal model of cerebral ischemia, the mechanism is not well understood. In this present study we investigated whether naloxone affected the mitochondrial apoptotic pathway in ischemic brain injury of rats. SD rats were subjected to a permanent middle cerebral artery occlusion surgery, and received naloxone (0.5, 1, 2 mg/kg, i.v.) immediately after ischemia. Neurological deficits were evaluated 24 h after ischemia using the McGraw Stroke Index, and then the rats were killed, and the brains were collected for further analyses. We show that naloxone treatment dose-dependently decreased the infarction volume and morphological injury, improved motor behavioral function, and markedly curtailed brain edema. Furthermore, naloxone administration significantly inhibited the nuclear translocation of NF-κB p65 and decreased the levels of nuclear NF-κB p65 in the ischemic penumbra. Naloxone administration also dose-dependently increased the NF-κB inhibitory protein (IκBα) levels and attenuated phosphorylated NIK and IKKα levels in the ischemic penumbra. In addition, naloxone administration dose-dependently increased Bcl-2 levels, decreased Bax levels, stabilized the mitochondrial transmembrane potential, and inhibited cytochrome c release and caspase 3 and caspase 9 activation. These results indicate that the neuroprotective effects of naloxone against ischemic brain injury involve the inhibition of NF-κB activation via the suppression of the NIK/IKKα/IκBα pathway and the obstruction of the mitochondrial apoptotic pathway in neurons. 展开更多
关键词 cerebral ischemia NALOXONE NF-ΚB mitochondrial APOPTOTIC pathway NEUROPROTECTION
Overexpression of steroid receptor coactivators alleviates hyperglycemia-induced endothelial cell injury in rats through activating the PI3K/Akt pathway
19
作者 Xiao-juan Quan Chun-lian Liang +2 位作者 Ming-zhu Sun Lin Zhang Xiu-li Li 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第5期648-657,共10页
Hyperglycemia is a major factor in vascular endothelial injury that finally leads to a cardiovascular event. Steroid receptor coactivators (SRCs) are a group of non-DNA binding proteins that induce structural changes ... Hyperglycemia is a major factor in vascular endothelial injury that finally leads to a cardiovascular event. Steroid receptor coactivators (SRCs) are a group of non-DNA binding proteins that induce structural changes in steroid receptors (nuclear receptors) critical for transcriptional activation. SRCs, namely, SRC-1, SRC-2, and SRC-3, are implicated in the regulation of vascular homeostasis. In this study we investigate the role of SRCs in hyperglycemia-induced endothelial injury. Aortic endothelial cells were prepared from normal and diabetic rats, respectively. Diabetic rats were prepared by injection of streptozotocin (50 mg/kg, i.p.). The expression levels of SRC-1 and SRC-3 were signi?cantly decreased in endothelial cells from the diabetic rats. Similar phenomenon was also observed in aortic endothelial cells from the normal rats treated with a high glucose (25 mM) for 4 h or 8 h. The expression levels of SRC-2 were little affected by hyperglycemia. Overexpression of SRC-1 and SRC-3 in high glucose-treated endothelial cells significantly increased the cell viability, suspended cell senescence, and inhibited cell apoptosis compared with the control cells. We further showed that overexpression of SRC-1 and SRC-3 markedly suppressed endothelial injury through restoring nitric oxide production, upregulating the expression of antioxidant enzymes (SOD, GPX, and CAT), and activating the PI3K/Akt pathway. The beneficial effects of SRC-1 and SRC-3 overexpression were blocked by treatment with the PI3K inhibitor LY294002 (10 mM) or with the Akt inhibitor MK-2206 (100 nM). In conclusion, hyperglycemia decreased SRC-1 and SRC-3 expression levels in rat aortic endothelial cells. SRC-1 and SRC-3 overexpression might protect against endothelial injury via inhibition of oxidative stress and activation of PI3K/Akt pathway. 展开更多
关键词 DIABETICS STREPTOZOTOCIN HYPERGLYCEMIA endothelial cells STEROID receptor coactivators CELL SENESCENCE CELL apoptosis oxidative stress PI3K/Akt pathway LY294002 MK-2206
基于通路及网络分析方法探究肝细胞肝癌差异表达基因
20
作者 岳晓粉 李建彪 +1 位作者 李爽 陆伟 《生物医学工程与临床》 CAS 2019年第2期212-221,共10页
目的基于通路及网络分析方法,对肝细胞肝癌(HCC)相关差异表达基因进行系统分析,旨在由分子、通路及网络层面理解HCC发生、发展机制,并对HCC的防治靶点进行预测,为后续实验提供一些有意义的信息。方法由公共基因表达数据库(GEO)下载4组与... 目的基于通路及网络分析方法,对肝细胞肝癌(HCC)相关差异表达基因进行系统分析,旨在由分子、通路及网络层面理解HCC发生、发展机制,并对HCC的防治靶点进行预测,为后续实验提供一些有意义的信息。方法由公共基因表达数据库(GEO)下载4组与HCC相关的生物基因芯片(GSE62232、GSE49515、GSE19665和GSE29721),利用相应R包对基因表达阵列的差异表达基因进行计算与比较,确定HCC相关差异表达基因。利用功能富集分析方法 ,对HCC相关差异表达基因进行生物过程及通路注释。利用网络分析方法,对HCC相关差异表达基因进行生物调控网络构建,并对关键基因进行筛选,同时利用分子复合物检测(MCODE)方法对HCC相关疾病模块进行搜索。结果确定442个HCC相关差异表达基因,这些差异表达基因主要调控细胞周期、核分裂、染色体分离及代谢相关生物过程;参与影响细胞周期通路、有丝分裂过程通路、生物氧化通路、金属离子反应通路、生物代谢通路等多条信号通路的正常信号转导。网络分析提示,HCC相关差异表达基因之间互作关系紧密,进一步分析鉴定出20个处于HCC调控网络中心的关键Hub基因,推测Hub基因是HCC防治靶点。同时,鉴定出5个与HCC紧密相关的疾病模块,分别为细胞周期处理模块、趋化因子介导的信号处理模块、血管生成调控模块、与氧化应激和代谢过程相关功能模块及细胞核分裂模块。结论利用生物信息学方法,可以系统分析HCC的发生、发展机制,并对其防治靶点进行预测,为临床诊疗及后续基础实验提供有价值的信息。 展开更多
关键词 肝细胞肝癌 差异表达基因 通路 网络分析 致病机制 药物靶点
上一页 1 2 250 下一页 到第
使用帮助 返回顶部 意见反馈