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生物肽不同结构肽链对生物修饰金属材料疏水性能的影响 预览
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作者 麻春英 袁成清 肖劲飞 《表面技术》 EI CAS CSCD 北大核心 2019年第1期141-146,共6页
目的通过调整与金属反应的肽的肽链结构,提高反应后金属表面的疏水性。方法取同种肽两种不同结构(L-型和D-型反序结构)分别与金属不锈钢作用,通过CA测定材料疏水性的变化,采用FTIR显示有机肽在材料表面的附着情况。分别在L-型和D-型肽... 目的通过调整与金属反应的肽的肽链结构,提高反应后金属表面的疏水性。方法取同种肽两种不同结构(L-型和D-型反序结构)分别与金属不锈钢作用,通过CA测定材料疏水性的变化,采用FTIR显示有机肽在材料表面的附着情况。分别在L-型和D-型肽上接入疏水基团十二烷酸,获得两种新的不同结构的肽:L.-型肽和D.-型肽。两种肽分别与金属不锈钢作用,利用FTIR和CA研究疏水基团的接入及其对材料疏水性的影响情况。结果与金属不锈钢作用之后,D型肽反序结构修饰的不锈钢表面接触角比L型肽修饰有所提高,由原来的39.7.变成80.1.。接入疏水基团后的肽在PBS中的溶解度减小,用有机溶剂初溶之后再溶入PBS中,生物肽全部溶解。该肽溶液与金属材料作用之后,D.型肽比L.型肽作用的表面接触角更高,分别为89.4.和87.4.,前者作用效果更好。结论通过调整生物肽肽链的结构,可以改变改性金属材料表面的疏水特性,为进一步通过调整金属反应肽的肽链结构和疏水基团的种类得到具有良好疏水性能的金属材料提供了有力的数据支撑。 展开更多
关键词 生物肽 肽链结构 手性异构 表面改性 疏水性 接触角
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Notoginseng Saponin Rg1 Prevents Cognitive Impairment through Modulating APP Processing in Aβ1-42-injected Rats 预览
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作者 Shang-zhi LIU Wei CHENG +6 位作者 Jia-wei SHAO Yun-fan GU Yi-yi ZHU Qi-jing DONG Si-yu BAI Ping WANG Li LIN 《当代医学科学(英文)》 SCIE CAS 2019年第2期196-203,共8页
With the intensification of the aging process of the world,Alzheimer's disease(AD),which is the main type of senile dementia,has become a primary problem in the present society.Lots of strategies have been used to... With the intensification of the aging process of the world,Alzheimer's disease(AD),which is the main type of senile dementia,has become a primary problem in the present society.Lots of strategies have been used to prevent and treat AD in animal nlodels and clinical trials,but most of them ended in failure.Panax notoginseng saponins(PNS)contain a variety of monomer compositions which have been separated and identified.Among of the monomer compositions,notoginseng saponin Rg1(Rg1)accounts for 20%of the cultivation of panax notoginseng roots.And now PNS have been reported to be widely used to treat cardicerebrovascular diseases and have neuroprotective effects to restrain theβ-amyloid peptide(Aβ)25-35-niediated apoptosis.Moreover,it is reported that PNS could accelerate the growth of nerve cells,increase the length of axons and promote synaptic plasticity.Whether Rg1 can ameliorate the cognitive impairment and the underlying mechanism has not been elucidated.To study the preventive effect of Rg1 on cognitive impairment and the possible mechanism,we established the cognitive impairment model in rats through Aβ1-42(2.6μg/μL,5μL)injection and then treated the rats with Rg1(25,50 and 100 mg/kg)administered intragastrically for 4 weeks.We observed that Aβ1-42 could induce spatial learning and memory deficits in rats.Simultaneously,Aβ1-42 injection also resulted in the reduced neuron number in comuammonis 1(CA1)and dentate gyrus(DG)of hippocampus,as well as the increased level of hyperphosphorylatedβ-amyloid precursor protein(APP)at Thr668 site with up-regulation ofβ-APP cleaving enzyme 1(BACE1)and presenilin 1(PS1)and down-regulation of a disintegrin and metalloprotease domain-containing protein 10(ADAM 10)and insulindegrading enzyme(IDE).Administration of Rg1 effectively rescued the cognitive impairment and neuronal loss,and inhibited theβ-secretase processing of APP through reducing APP-Thr668 phosphorylation and BACE1/PS1 expression,and increasing the expression of ADAM 10 and IDE.We concluded that Rg1 migh 展开更多
关键词 notoginseng SAPONIN RG1 Alzheimer's disease spatial learning and memory deficits Β-AMYLOID peptide SECRETASE degrading cnzyme
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多肽药物关键水解酶体外高通量分析方法的建立 预览
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作者 张凡 田浤 姚文兵 《中国药科大学学报》 CAS CSCD 北大核心 2019年第3期352-356,共5页
为了建立测定多肽药物体内代谢关键水解酶的高通量分析方法,本研究基于高效液相色谱技术,确定通用的体外酶解反应条件为:pH7.8或9.0,缓冲体系为0.01mol/LPBS或50mmol/L Tris缓冲液,实现对多肽药物关键水解酶的统一高通量体外检测。利用... 为了建立测定多肽药物体内代谢关键水解酶的高通量分析方法,本研究基于高效液相色谱技术,确定通用的体外酶解反应条件为:pH7.8或9.0,缓冲体系为0.01mol/LPBS或50mmol/L Tris缓冲液,实现对多肽药物关键水解酶的统一高通量体外检测。利用该方法对多肽药物普兰林肽的关键水解酶进行分析,研究结果显示,基肽释放相关酶5和二肽基肽酶4对普兰林肽的水解作用最强,与微量热泳动分析结果一致。因此,本研究所建立的方法可用于分析多肽药物的关键水解酶,为优化多肽药物的酶稳定性提供方法参考与指导。 展开更多
关键词 多肽 酶解代谢 高效液相色谱 蛋白水解酶 高通量分析 普兰林肽
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Cleavage of the Babuvirus Movement Protein B4 into Functional Peptides Capable of Host Factor Conjugation is Required for Virulence
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作者 Jun Zhuang Wenwu Lin +4 位作者 Christopher J.Coates Pengxiang Shang Taiyun Wei Zujian Wu Lianhui Xie 《中国病毒学:英文版》 CAS CSCD 2019年第3期295-305,共11页
Banana bunchy top virus(BBTV)poses a serious danger to banana crops worldwide.BBTV-encoded protein B4 is a determinant of pathogenicity.However,the relevant molecular mechanisms underlying its effects remain unknown.I... Banana bunchy top virus(BBTV)poses a serious danger to banana crops worldwide.BBTV-encoded protein B4 is a determinant of pathogenicity.However,the relevant molecular mechanisms underlying its effects remain unknown.In this study,we found that a functional peptide could be liberated from protein B4,likely via proteolytic processing.Site-directed mutagenesis indicated that the functional processing of protein B4 is required for its pathogenic effects,including dwarfism and sterility,in plants.The released protein fragment targets host proteins,such as the large subunit of RuBisCO(RbcL)and elongation factor 2(EF2),involved in protein synthesis.Therefore,the peptide released from B4(also a precursor)may act as a non-canonical modifier to influence host-pathogen interactions involving BBTV and plants. 展开更多
关键词 BANANA bunchy top virus(BBTV) MOVEMENT protein B4 Functional peptide PATHOGENICITY
基于纳升电喷雾离子化质谱技术(nano ESI-MS)建立登革病毒检测方法
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作者 王玉娜 刘丽娟 +2 位作者 李丽丽 张丽萍 赵景波 《中国国境卫生检疫杂志》 CAS 2019年第1期5-8,共4页
目的建立基于纳升电喷雾离子化质谱技术(nano ESI-MS)检测登革病毒的方法。方法采用生物工程技术,合成Ⅱ型登革病毒(DENV-Ⅱ)NS1蛋白特异性肽段作为识别标志,通过优化实验条件,建立基于nano ESI-MS技术的DENV-ⅡNS1蛋白特异性肽段的检... 目的建立基于纳升电喷雾离子化质谱技术(nano ESI-MS)检测登革病毒的方法。方法采用生物工程技术,合成Ⅱ型登革病毒(DENV-Ⅱ)NS1蛋白特异性肽段作为识别标志,通过优化实验条件,建立基于nano ESI-MS技术的DENV-ⅡNS1蛋白特异性肽段的检测方法;借助特异性胰蛋白酶酶解技术,实现对DENV-ⅡNS1蛋白的检测验证。结果在2.5 kV电压、以甲醇、水按体积比6∶4比例并加入0.1%甲酸作为蛋白稀释液条件下,应用nano ESI-MS技术可成功检测出DENV-ⅡNS1蛋白特异性标志性肽段,灵敏度达10 ng/ml。结论 nano ESI-MS技术灵敏度高,操作简单,可在敞开式大气压环境下实现检测。该方法的建立为扩展nano ESI-MS技术应用范围提供了新思路。 展开更多
关键词 纳升电喷雾离子化质谱 登革病毒 非结构蛋白 肽段
Cytoprotective Mechanism of the Novel Gastric Peptide BPC157 in Gastrointestinal Tract and Cultured Enteric Neurons and Glial Cells
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作者 Xi-Yu Wang Meihua Qu +6 位作者 Rui Duan Dengping Shi Ling Jin Jinping Gao Jackie D.Wood Junhua Li Guo-Du Wang 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第1期167-170,共4页
Dear Editor,Body protection compound (BPC) 157 is a stable gastric pentadecapeptide. Predrag Sikiric’s team has carried out many investigations of its cytoprotective effects in different organs and tissues (1, 2)Thei... Dear Editor,Body protection compound (BPC) 157 is a stable gastric pentadecapeptide. Predrag Sikiric’s team has carried out many investigations of its cytoprotective effects in different organs and tissues (1, 2)Their evidence indicates that BPC157 has potent cytoprotection in neural injury and gastrointestinal (GI) ulcers. Nevertheless. 展开更多
关键词 BPC HT GI CYTOPROTECTIVE Mechanism of the NOVEL GASTRIC PEPTIDE BPC157 in Gastrointestinal Tract and CULTURED ENTERIC Neurons and Glial Cells
Identifying peptides that specifically bind to MDA-MB-468 breast cancer cells 预览
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作者 Xiaoli Dai Qing Zhang +1 位作者 Huili Zhang Hongtao Xu 《肿瘤学与转化医学:英文版》 2019年第3期119-122,共4页
Objective To use phage display technique to screen for small polypeptides that specifically bind to MDA-MB-468 cells.Methods A random heptapeptide phage display library was used for in vitro screening against target M... Objective To use phage display technique to screen for small polypeptides that specifically bind to MDA-MB-468 cells.Methods A random heptapeptide phage display library was used for in vitro screening against target MDA-MB-468 cells.SC1180 cells were used for subtractive selection.High-affinity phage DNA was extracted,and peptides were sequenced.Results(1)The original library capacity of the polypeptide library was 2×10^13 pfu/mL,and phage titer was determined over 4 rounds.The average library capacity was 1.8×10^13 pfu/mL.(2)Subtractive screening showed that the phage library volume of each round was 1.8×10^12 pfu/mL,and that there was an enrichment effect in each subsequent round.Screening was stopped after the fourth round.(3)PCR results showed that the size of 39 products(78.0%)and 11 products(22%),were 300 bp and 258 bp,respectively.Thirty positive phages were selected for DNA extraction and sequencing,and the corresponding amino acid sequence was LMTRXSK.The sequence had no homology with known genes or proteins.Conclusion Using the phage display technique,we identified that the short polypeptide,LMTRXSK,specifically binds MDA-MB-468 human breast cancer cells. 展开更多
关键词 PHAGE BREAST cancer specific binding PEPTIDE
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酶解大豆分离蛋白的抗原活性变化及其抗原表位分析 预览
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作者 王章存 袁路阳 +3 位作者 张露 胡金强 安广杰 赵学伟 《食品科学》 EI CAS CSCD 北大核心 2019年第10期64-69,共6页
研究酶解过程中大豆蛋白分子成分和抗原性变化规律及酶解物中是否存在抗原表位序列。用碱性蛋白酶水解大豆分离蛋白后,首先分别用电泳和酶联免疫法分析蛋白质分子组分和抗原活性变化,结果发现,酶解过程中新生成23 ku和21 ku抗酶解肽链... 研究酶解过程中大豆蛋白分子成分和抗原性变化规律及酶解物中是否存在抗原表位序列。用碱性蛋白酶水解大豆分离蛋白后,首先分别用电泳和酶联免疫法分析蛋白质分子组分和抗原活性变化,结果发现,酶解过程中新生成23 ku和21 ku抗酶解肽链直到酶解120min时仍未消失,同时酶解物中剩余28%抗原活性。进一步通过胰蛋白酶对这两个肽链胶内酶解并用基质辅助激光解析串联飞行时间质谱仪分析后,将所得肽段氨基酸序列分别与抗原数据库中抗原表位序列和大豆分离蛋白氨基酸序列进行匹配解析,结果发现,在21 ku组分中含有两个完整的序列抗原表位(LQRFNQRSPQLQNLR和SEDKPFNLRSRDPIYSNKLGKFFEITPEKN),且均来自β-伴大豆球蛋白中的α-亚基,21 ku组分抗原剩余率为15.7%;在23 ku肽链中含有抗原表位的部分氨基酸序列,但未发现含有完整的抗原表位序列,23 ku组分抗原剩余率为2.1%,该组分中是否含有新的未知抗原表位或是否与糖链有关尚待深入研究。上述研究结果揭示了大豆蛋白酶解物中仍残留抗原性的部分原因。 展开更多
关键词 大豆分离蛋白 酶解 抗原活性 抗原表位
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呈味氨基酸和肽对发酵食品中风味的作用 预览
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作者 耿瑞蝶 王金水 《中国调味品》 CAS 北大核心 2019年第7期176-178,183共4页
发酵食品因其独特的风味及营养价值一直受到人们的高度重视。蛋白质经酶促水解产生的味觉活性氨基酸、氨基酸衍生物及肽等风味化合物是发酵食品中主要的呈味物质,除了具有良好的风味以外,其抗高血压、抗氧化及抗癌等功能特性也逐渐受到... 发酵食品因其独特的风味及营养价值一直受到人们的高度重视。蛋白质经酶促水解产生的味觉活性氨基酸、氨基酸衍生物及肽等风味化合物是发酵食品中主要的呈味物质,除了具有良好的风味以外,其抗高血压、抗氧化及抗癌等功能特性也逐渐受到广泛的关注。文章主要介绍了发酵食品中能够呈现不同风味的氨基酸和肽及其功能特性。 展开更多
关键词 发酵食品 氨基酸 呈味特性
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Biomarkers enhance the long-term predictive ability of the KAMIR risk score in Chinese patients with ST-elevation myocardial infarction
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作者 Jian-Jun Wang Yan Fan +5 位作者 Yan Zhu Jian-Dong Zhang Su-Mei Zhang Zhao-Fei Wan Hong-Ling Su Na Jiang 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第1期30-41,共12页
Background:The Global Registry of Acute Coronary Events (GRACE) score is recommended by current ST-elevation myocardial infarction (STEMI) guidelines.But it has inherent defects.The present study aimed to investigate ... Background:The Global Registry of Acute Coronary Events (GRACE) score is recommended by current ST-elevation myocardial infarction (STEMI) guidelines.But it has inherent defects.The present study aimed to investigate the more compatible risk stratification for Chinese patients with STEMI and to determine whether the addition of biomarkers to the Korea Acute Myocardial Infarction Registry (KAMIR) score could enhance its predictive value for long-term outcomes.Methods:A total of 1093 consecutive STEMI patients were included and followed up 48.2 months.Homocysteine,hypersensitive C-reactive protein (hs-CRP),and N-terminal pro-B-type natriuretic peptide (NT-proBNP) were detected.The KAMIR score and the GRACE score were calculated.The performance between the KAMIR and the GRACE was compared.The predictive power of the KAMIR alone and combined with biomarkers were assessed by the receiver-operating characteristic (ROC) curve.Results:The KAMIR demonstrated a better risk stratification and predictive ability than the GRACE (death:AUC = 0.802 vs.0.721,P<0.001;major adverse cardiovascular events (MACE):AUC = 0.683 vs.0.656,P<0.001).It showed that the biomarkers could independently predict death [homocysteine:HR= 1.019 (1.015–1.024),P<0.001;hs-CRP:HR= 1.052 (1.000–1.104),P= 0.018;NT-pro BNP:HR= 1.142 (1.004–1.280),P= 0.021] and MACE [homocysteine:HR= 1.019 (1.015–1.024),P<0.001;hs-CRP:HR= 1.012 (1.003–1.021),P= 0.020;NT-pro BNP:HR= 1.136 (1.104–1.168),P= 0.006].When they were used in combination with the KAMIR,the area under the ROC curve (AUC) significantly increased for death [homocysteine:AUC = 0.802 vs.0.890,Z = 5.982,P<0.001;hs-CRP:AUC = 0.802 vs.0.873,Z= 3.721,P<0.001;NT-pro BNP:AUC= 0.802 vs.0.871,Z = 2.187,P= 0.047;homocysteine,hs-CRP and NT-pro BNP:AUC = 0.802 vs.0.940,Z = 6.177,P<0.001] and MACE [homocysteine:AUC = 0.683 vs.0.771,Z= 6.818,P<0.001;hs-CRP:AUC= 0.683 vs.0.712,Z= 2.022,P= 0.031;NT-pro BNP:AUC= 0.683 vs.0.720,Z= 2.974,P= 0.003;homocysteine,hs-CRP and NT-pro BNP:AUC= 0.683 vs.0.789,Z= 6.900, 展开更多
关键词 ST-elevation MYOCARDIAL INFARCTION the Korea ACUTE MYOCARDIAL INFARCTION REGISTRY RISK SCORE the Global REGISTRY of ACUTE Coronary Events RISK SCORE homocysteine hypersensitive C-reactive protein N-terminal pro-B-type natriuretic peptide
棉花CLE多肽家族的全基因组鉴定与生物信息学分析 预览
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作者 袁娜 李阳 +2 位作者 杨郁文 张保龙 杜建厂 《棉花学报》 CSCD 北大核心 2019年第4期263-281,共19页
【目的】CLE(CLAVATA3/Embryo surrounding region-related)多肽是一类小分子分泌蛋白,在植物生长发育过程中,对于维持分生组织细胞增殖与分化间的平衡起到重要的信号转导和调控作用。CLE家族是迄今为止报道的最大的植物多肽分子家族,... 【目的】CLE(CLAVATA3/Embryo surrounding region-related)多肽是一类小分子分泌蛋白,在植物生长发育过程中,对于维持分生组织细胞增殖与分化间的平衡起到重要的信号转导和调控作用。CLE家族是迄今为止报道的最大的植物多肽分子家族,也是近十年来研究最为热门的植物多肽激素。尽管CLE基因家族在拟南芥和水稻等模式植物中取得较多的研究进展,但在棉花中有关CLE多肽基因家族的研究尚属空白。通过对CLE多肽家族的鉴定及演化研究,可以为进一步挖掘棉花中的功能多肽及其信号转导研究提供一定的理论基础。【方法】本文以亚洲棉(Gossypium arboreum L.)、雷蒙德氏棉(G. raimondii Ulbrich)、陆地棉(G. hirsutum L.)和海岛棉(G. barbadense L.)为研究对象,利用生物信息学方法和已鉴定的拟南芥CLE基因序列,在棉花基因组中进行同源比对,并对4个棉种中的CLE家族成员进行全基因组鉴定和分析。【结果】共得到148个棉花CLE候选基因。陆地棉和海岛棉中CLE基因数量都分别是亚洲棉和雷蒙德氏棉的2倍。聚类分析将所有CLE基因分为5个亚组。Ka/Ks分析表明大部分基因都经历了负选择作用,有11对基因经历了显著的正选择作用。转录组数据分析发现,除海岛棉中的GbCLE39、GbCLE13、GbCLE43,陆地棉中GhCLE34、GhCLE9以及亚组棉中的GaCLE4外,大部分CLE基因在棉花组织中的表达量较低,这与多肽在植物体内含量较低相一致。CLE核心保守基序分析发现了12个棉花特有的多肽,其中有2个多肽来源于受到强烈正选择作用的基因,因此在后续研究中可以对其进行进一步深入分析。【结论】本文利用生物信息学、比较基因组学等方法,首次对棉花的CLE基因家族进行了鉴定和分析,对于进一步挖掘棉花中的功能多肽及其信号调控网络研究提供了一定的理论基础。 展开更多
关键词 CLE基因 多肽 棉花 生物信息学
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南极磷虾自溶前后氨基酸和胰蛋白酶降解产物的变化 预览
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作者 胡玲萍 张晓梅 +5 位作者 张鸿伟 孙维维 温运启 林黎明 薛长湖 姜晓明 《食品科学》 EI CAS CSCD 北大核心 2019年第11期1-6,共6页
南极磷虾富含内源自溶酶,死后易快速自溶,限制了其加工利用。为研究南极磷虾自溶特性,测定了不同条件下南极磷虾自溶酶的最适温度和pH值,然后在最适自溶条件下研究了南极磷虾自溶3 h前后的氨基酸态氮含量和组成变化,并对南极磷虾自溶前... 南极磷虾富含内源自溶酶,死后易快速自溶,限制了其加工利用。为研究南极磷虾自溶特性,测定了不同条件下南极磷虾自溶酶的最适温度和pH值,然后在最适自溶条件下研究了南极磷虾自溶3 h前后的氨基酸态氮含量和组成变化,并对南极磷虾自溶前后的胰蛋白酶降解产物进行了分析。结果表明:南极磷虾自溶酶最适温度为55℃,最适pH值为7.5,在此条件下南极磷虾自溶后氨基酸态氮质量浓度升高;甘氨酸和脯氨酸为南极磷虾的主要游离氨基酸,其次为谷氨酸和丙氨酸,南极磷虾自溶后鲜味氨基酸如谷氨酸、甘氨酸、丙氨酸质量分数降低,而苦味氨基酸如亮氨酸、异亮氨酸、酪氨酸和苯丙氨酸质量分数升高;南极磷虾自溶后胰蛋白酶降解产物分子质量分布向小分子质量范围偏移。本实验为进一步研究南极磷虾自溶特性提供了理论依据。 展开更多
关键词 南极磷虾 自溶 氨基酸 胰蛋白酶 多肽
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Interleukin-4 affects microglial autophagic flux 预览
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作者 Run-Hong Tang Rui-Qun Qi Hua-Yan Liu 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1594-1602,共9页
Interleukin-4 plays an important protective role in Alzheimer’s disease by regulating microglial phenotype,phagocytosis of amyloid-β,and secretion of anti-inflammatory and neurotrophic cytokines.Recently,increasing ... Interleukin-4 plays an important protective role in Alzheimer’s disease by regulating microglial phenotype,phagocytosis of amyloid-β,and secretion of anti-inflammatory and neurotrophic cytokines.Recently,increasing evidence has suggested that autophagy regulates innate immunity by affecting M1/M2 polarization of microglia/macrophages.However,the role of interleukin-4 in microglial autophagy is unknown.In view of this,BV2 microglia were treated with 0,10,20 or 50 ng/mL interleukin-4 for 24,48,or 72 hours.Subsequently,light chain 3-II and p62 protein expression levels were detected by western blot assay.BV2 microglia were incubated with interleukin-4(20 ng/mL,experimental group),3-methyladenine(500μM,autophagy inhibitor,negative control group),rapamycin(100 nM,autophagy inductor,positive control group),3-methyladenine+interleukin-4(rescue group),or without treatment for 24 hours,and then exposed to amyloid-β(1μM,model group)or vehicle control(control)for 24 hours.LC3-II and p62 protein expression levels were again detected by western blot assay.In addition,expression levels of multiple markers of M1 and M2 phenotype were assessed by real-time fluorescence quantitative polymerase chain reaction,while intracellular and supernatant amyloid-βprotein levels were measured by enzyme-linked immunosorbent assay.Our results showed that interleukin-4 induced microglial autophagic flux,most significantly at 20 ng/mL for 48 hours.Interleukin-4 pretreated microglia inhibited blockade of amyloid-β-induced autophagic flux,and promoted amyloid-βuptake and degradation partly through autophagic flux,but inhibited switching of amyloid-β-induced M1 phenotype independent on autophagic flux.These results indicate that interleukin-4 pretreated microglia increases uptake and degradation of amyloid-βin a process partly mediated by autophagy,which may play a protective role against Alzheimer’s disease. 展开更多
关键词 nerve REGENERATION Alzheimer’s disease INTERLEUKIN-4 amyloid-β MICROGLIAL autophagy MICROGLIAL polarization MICROGLIA M1 PHENOTYPE M2 PHENOTYPE peptide degradation neural REGENERATION
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Protective potential of glucagon like peptide 2(GLP-2) against the neurodegeneration 预览
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作者 Antonella Amato Flavia Mule 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1901-1902,共2页
Neurodegeneration consists in loss of neuron specific types, pattern and distribution, leading to progressive dysfunctions of the central nervous system. Neurodegenerative diseases include diverse pathological conditi... Neurodegeneration consists in loss of neuron specific types, pattern and distribution, leading to progressive dysfunctions of the central nervous system. Neurodegenerative diseases include diverse pathological conditions, among which Alzheimer’s and Parkinson’s diseases are the most prevalent ones. Alzheimer’s disease is known as a growing dementia, characterized by progressive language, memory, and cognitive loss, while Parkinson’s disease is primarily characterized as a motor disorder. Senile plaques, caused by amyloid β peptide, hyperphosphorylated taubased neurofibrillary tangles and synapse loss, are the principal pathological hallmarks of Alzheimer’s disease. 展开更多
关键词 Protective POTENTIAL GLUCAGON LIKE PEPTIDE 2(GLP-2) NEURODEGENERATION
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响应面法优化燕窝不溶性蛋白酶解工艺的研究 预览
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作者 郑心怡 李致瑜 +2 位作者 曾红亮 郑宝东 张怡 《中国农学通报》 2019年第6期151-157,共7页
研究旨在应用酶解技术将燕窝中不溶性蛋白转换为水溶性多肽,有利于人体消化吸收,以期为燕窝新产品的开发奠定基础。以水解度和肽得率为指标,筛选出碱性蛋白酶对燕窝中不溶性蛋白进行酶解。通过单因素实验研究酶解时间、酶加量、底物浓... 研究旨在应用酶解技术将燕窝中不溶性蛋白转换为水溶性多肽,有利于人体消化吸收,以期为燕窝新产品的开发奠定基础。以水解度和肽得率为指标,筛选出碱性蛋白酶对燕窝中不溶性蛋白进行酶解。通过单因素实验研究酶解时间、酶加量、底物浓度对燕窝多肽得率的影响,并利用响应曲面法对燕窝不溶性蛋白酶解工艺进行优化。酶解的最佳工艺为:底物浓度为2.5%,酶加量为4%,酶解时间为3.6 h,肽得率为33.59%。通过试验对该模型进行验证:得到肽得率为33.38%,相对误差为0.56%,说明该模型能较好地反映出燕窝不溶性蛋白酶解情况。此外,通过凝胶电泳试验证明燕窝不溶性蛋白通过碱性蛋白酶酶解,分子量有效降低。该试验获得了燕窝多肽,为后续产品进一步深入开发和应用提供一定理论基础。 展开更多
关键词 燕窝 多肽 响应曲面 凝胶电泳
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蛋白质组学在人乳蛋白质研究中的应用 预览
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作者 于文皓 祁艳霞 靳艳 《色谱》 CAS CSCD 北大核心 2019年第5期471-476,共6页
人乳是新生儿最理想的天然食物,蛋白质是人乳中最主要的营养成分之一。随着蛋白质组学技术的发展,利用蛋白质组学的方法研究人乳蛋白质也取得了一些研究成果。本文综述了近年来蛋白质组学技术在人乳蛋白质研究中的应用,分别从人乳蛋白... 人乳是新生儿最理想的天然食物,蛋白质是人乳中最主要的营养成分之一。随着蛋白质组学技术的发展,利用蛋白质组学的方法研究人乳蛋白质也取得了一些研究成果。本文综述了近年来蛋白质组学技术在人乳蛋白质研究中的应用,分别从人乳蛋白质的组成研究、动态变化、人乳与其他来源乳汁的蛋白质差异比较、人乳磷酸化蛋白和糖基化蛋白研究、人乳内源肽的研究及人乳蛋白与疾病等几个方面进行了阐述。蛋白质组学技术使人乳蛋白质的研究进入了微量营养研究的时代,人乳蛋白质组学的研究成果将为母婴健康提供更好的保障。 展开更多
关键词 人乳 蛋白质组学 蛋白质 综述
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预消化的肠内营养对ICU危重症患者的营养状况及肠内营养耐受性的影响
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作者 孙丽娟 薛森海 +3 位作者 闫凤 代玉洁 徐赤红 赵长海 《现代生物医学进展》 CAS 2019年第10期1883-1888,共6页
目的:研究预消化的肠内营养制剂对ICU危重症患者营养状况及肠内营养耐受性的影响。方法:本试验为前瞻、随机、对照、单盲研究,将符合入组条件的ICU患者随机分为试验组和对照组。试验组给予预消化型肠内营养制剂,而对照组给予整蛋白型肠... 目的:研究预消化的肠内营养制剂对ICU危重症患者营养状况及肠内营养耐受性的影响。方法:本试验为前瞻、随机、对照、单盲研究,将符合入组条件的ICU患者随机分为试验组和对照组。试验组给予预消化型肠内营养制剂,而对照组给予整蛋白型肠内营养制剂进行营养支持。比较两组患者入组及营养支持2周后的营养指标、肠内营养耐受性及ICU住院天数等。结果:治疗2周后,试验组前白蛋白、转铁蛋白较对照组明显升高(P<0.05),肠内营养不耐受、腹内压升高、腹泻、肠鸣音减弱及胃肠道出血发生率较对照组明显降低(P<0.05),ICU住院时间也较对照组明显缩短(P<0.05)。结论:预消化的肠内营养制剂对ICU患者有良好的治疗效果,可降低胃肠道不良反应发生率,提高耐受性,明显改善患者营养状况。 展开更多
关键词 肠内营养 短肽 危重症患者
Relationship between different healing degree and C peptide in elderly patients with diabetic foot ulcers
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作者 Lin Qu Ya-Li Zhou +1 位作者 Teng Zhou Yan-Hu Dong 《海南医科大学学报(英文版)》 2019年第4期50-55,共6页
Objective:To explore the relationship between different healing degree and C peptide in elderly patients with diabetic foot ulcers.Methods:Totally, two hundred and forty-one DFU patients were selected into our researc... Objective:To explore the relationship between different healing degree and C peptide in elderly patients with diabetic foot ulcers.Methods:Totally, two hundred and forty-one DFU patients were selected into our research from September 2nd, 2011 to September 2nd, 2012. After admission, the patients' sex, age, the course of diabetes, the days of hospitalization, and BMI were recorded. After examination, blood routine and blood biochemistry, urine routine, hepatorenal work were recorded. Follow up 5 years, patients with unhealed ulcers were selected into bad prognosis group during follow-up, and patients with ulcerative healing into good prognosis group. Single factor analysis showed factors affecting the healing of DFU patients;Cox proportional risk model was further evaluated the effect of all factors with statistical significance on the healing of DFU patients. Using the ROC curve to analyze the statistically significant factors and to calculate the area under the curve to evaluate the predictive ability of the healing of the patients with DFU.Results: At the end of the follow-up, 6 patients were lost, 177 had the end point, and the healing rate was 73.44%. Single factor and multifactor analysis showed that the course of diabetes and HbA1C were independent risk factors affecting the prognosis. FCP was an independent protective factor affecting the prognosis. In ROC, the AUC of diabetes course was 0.754 under the ROC curve. The AUC of HbA1C under the line is 0.791, while the AUC of FCP level is 0.581 under the ROC curve.Conclusion: Low FCP is an independent risk factor for DFU healing, but FCP has little predictive effect on patient healing. 展开更多
关键词 DIABETIC FOOT C PEPTIDE OLD age HEALING
Effect of Jiawei Shenfu decoction on tumor necrosis factor-alpha and nuclear factor-kappa B in patients who have chronic heart failure with syndromes of deficiency of heart Yang
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作者 Yu Mei Guo Hangyuan +3 位作者 Ye Lingling Bian Jiaping Ma Lijuan Zheng Chunli 《中医杂志:英文版》 SCIE CAS CSCD 2019年第3期418-424,共7页
OBJECTIVE:To examine the clinical efficacy of Jiawei Shenfu decoction on tumor necrosis factor-alpha (TNF-cα) and nuclear factor-kappa B (NF-KB) levels in patients who have chronic heart failure with syndromes of def... OBJECTIVE:To examine the clinical efficacy of Jiawei Shenfu decoction on tumor necrosis factor-alpha (TNF-cα) and nuclear factor-kappa B (NF-KB) levels in patients who have chronic heart failure with syndromes of deficiency of heart Yang.METHODS:A total of 63 patients with syndromes of deficiency of heart Yang (chronic heart failure)were enrolled.Patients were randomly divided into the control group and Jiawei Shenfu group.All patients received standard medications for treatment of chronic heart failure.Patients in the Jiawei Shenfu group were additionally provided Jiawei Shenfu decoction one dose daily.Treatments continued for 4 consecutive weeks.The primary endpoint was the change in plasma B-type natriuretic peptide (BNP),NF-KB,and TNF-cα levels during 4 weeks of treatment.RESULTS:At the 4-week follow-up,a significant reduction in BNP levels compared with baseline was observed in both groups,but the Jiawei Shenfu decoction group showed a significantly greater reduction than did the control group.The Jiawei Shenfu group also showed superior performance regarding the Minnesota Living with Heart Failure Questionnaire score,the Chinese medicine syndrome score,heart rate,left ventricular ejection fraction,and 6-min walking distance compared with the control group.The degree of changes in NF-KB and TNF-α levels in the Jiawei Shenfu group was more significant than that in the control group.CONCLUSION:Routine medicine combined with Jiawei Shenfu decoction for patients with heart Yang deficiency syndrome in chronic heart failure can improve the left ventricular ejection fraction and cardiac function,and reduce BNP levels.The mechanism may be related to inhibition of pro-inflammatory cytokines and the NF-KB-induced kinase pathway,leading to amelioration of the inflammatory response. 展开更多
关键词 HEART failure NATRIURETIC peptide brain Tumor necrosis FACTOR-ALPHA NF-KAPPA B Heart-Yang DEFICIENCY Jiawei SHENFU decoction
Tauroursodeoxycholic acid(TUDCA) inhibits influenza A viral infection by disrupting viral proton channel M2
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作者 Ning Li Yanxu Zhang +9 位作者 Shuangxiu Wu Ruodan Xu Zhiqing Li Jindong Zhu Hongliang Wang Xiao Li Mingyao Tian Huijun Lu Ningyi Jin Chengyu Jiang 《科学通报:英文版》 SCIE EI CSCD 2019年第3期180-188,共9页
Influenza is a persistent threat to human health and there is a continuing requirement for updating antiinfluenza strategies. Initiated by observations of different endoplasmic reticulum(ER) responses of host to seaso... Influenza is a persistent threat to human health and there is a continuing requirement for updating antiinfluenza strategies. Initiated by observations of different endoplasmic reticulum(ER) responses of host to seasonal H1N1 and highly pathogenic avian influenza(HPAI) A H5N1 infections, we identified an alternative antiviral role of tauroursodeoxycholic acid(TUDCA), a clinically available ER stress inhibitor, both in vitro and in vivo. Rather than modulating ER stress in host cells, TUDCA abolished the proton conductivity of viral M2 by disrupting its oligomeric states, which induces inefficient viral infection. We also showed that M2 penetrated cells, whose intracellular uptake depended on its proton channel activity,an effect observed in both TUDCA and M2 inhibitor amantadine. The identification and application of TUDCA as an inhibitor of M2 proton channel will expand our understanding of IAV biology and complement current anti-IAV arsenals. 展开更多
关键词 INFLUENZA TUDCA M2 proton channel Virus ENTRY Cell-penetrating peptide OLIGOMERIZATION inhibitor
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