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Novel miRNA,miR-sc14,promotes Schwann cell proliferation and migration 预览
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作者 Xi-Meng Ji Shan-Shan Wang +5 位作者 Xiao-Dong Cai Xing-Hui Wang Qian-Yan Liu Pan Wang Zhang-Chun Cheng Tian-Mei Qian 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第9期1651-1656,共6页
MicroRNAs refer to a class of endogenous,short non-coding RNAs that mediate numerous biological functions.MicroRNAs regulate various physiological and pathological activities of peripheral nerves,including peripheral ... MicroRNAs refer to a class of endogenous,short non-coding RNAs that mediate numerous biological functions.MicroRNAs regulate various physiological and pathological activities of peripheral nerves,including peripheral nerve repair and regeneration.Previously,using a rat sciatic nerve injury model,we identified many functionally annotated novel microRNAs,including miR-sc14.Here,we used real-time reverse transcription-polymerase chain reaction to examine miR-sc14 expression in rat sciatic nerve stumps.Our results show that miRsc14 is noticeably altered following sciatic nerve injury,being up-regulated at 1 day and diminished at 7 days.EdU and transwell chamber assay results showed that miR-sc14 mimic promoted proliferation and migration of Schwann cells,while miR-sc14 inhiThe study was approved by the Jiangsu Provincial Laboratory Animal Management Committee,China on March 4,2015(approval No.20150304-004).bitor suppressed their proliferation and migration.Additionally,bioinformatic analysis examined potential target genes of miR-sc14,and found that fibroblast growth factor receptor 2 might be a potential target gene.Specifically,our results show changes of miR-sc14 expression in the sciatic nerve of rats at different time points after nerve injury.Appropriately,up-regulation of miR-sc14 promoted proliferation and migration of Schwann cells.Consequently,miR-sc14 may be an intervention target to promote repair of peripheral nerve injury.The study was approved by the Jiangsu Provincial Laboratory Animal Management Committee,China on March 4,2015(approval No.20150304-004). 展开更多
关键词 NERVE REGENERATION novel microRNAs miR-sc14 PERIPHERAL NERVE injury cell PROLIFERATION cell MIGRATION Schwann cells fibroblast growth factor receptor 2 biological functions PERIPHERAL NERVE REGENERATION regulatory mechanisms neural REGENERATION
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Comparative study of microarray and experimental data on Schwann cells in peripheral nerve degeneration and regeneration:big data analysis 预览
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作者 Ulfuara Shefa Junyang Jung 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期1099-1104,共6页
A Schwann cell has regenerative capabilities and is an important cell in the peripheral nervous system.This microarray study is part of a bioinformatics study that focuses mainly on Schwann cells.Microarray data provi... A Schwann cell has regenerative capabilities and is an important cell in the peripheral nervous system.This microarray study is part of a bioinformatics study that focuses mainly on Schwann cells.Microarray data provide information on differences between microarray-based and experiment-based gene expression analyses.According to microarray data,several genes exhibit increased expression(fold change)but they are weakly expressed in experimental studies(based on morphology,protein and mRNA levels).In contrast,some genes are weakly expressed in microarray data and highly expressed in experimental studies;such genes may represent future target genes in Schwann cell studies.These studies allow us to learn about additional genes that could be used to achieve targeted results from experimental studies.In the current big data study by retrieving more than 5000 scientific articles from PubMed or NCBI,Google Scholar,and Google,1016(up-and downregulated)genes were determined to be related to Schwann cells.However,no experiment was performed in the laboratory;rather,the present study is part of a big data analysis.Our study will contribute to our understanding of Schwann cell biology by aiding in the identification of genes.Based on a comparative analysis of all microarray data,we conclude that the microarray could be a good tool for predicting the expression and intensity of different genes of interest in actual experiments. 展开更多
关键词 Schwann cells big data analysis PERIPHERAL NERVE DEGENERATION PERIPHERAL NERVE REGENERATION MICROARRAY matched GENES promising GENES gene ranking
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On the development of optical peripheral nerve interfaces 预览
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作者 Hans E.Anderson Richard F.ff.Weir 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第3期425-436,共12页
Limb loss and spinal cord injury are two debilitating conditions that continue to grow in prevalence.Prosthetic limbs and limb reanimation present two ways of providing affected individuals with means to interact in t... Limb loss and spinal cord injury are two debilitating conditions that continue to grow in prevalence.Prosthetic limbs and limb reanimation present two ways of providing affected individuals with means to interact in the world.These techniques are both dependent on a robust interface with the peripheral nerve.Current methods for interfacing with the peripheral nerve tend to suffer from low specificity,high latency and insufficient robustness for a chronic implant.An optical peripheral nerve interface may solve some of these problems by decreasing invasiveness and providing single axon specificity.In order to implement such an interface three elements are required:(1)a transducer capable of translating light into a neural stimulus or translating neural activity into changes in fluorescence,(2)a means for delivering said transducer and (3)a microscope for providing the stimulus light and detecting the fluorescence change.There are continued improvements in both genetically encoded calcium and voltage indicators as well as new optogenetic actuators for stimulation.Similarly,improvements in specificity of viral vectors continue to improve expression in the axons of the peripheral nerve.Our work has recently shown that it is possible to virally transduce axons of the peripheral nerve for recording from small fibers.The improvements of these components make an optical peripheral nerve interface a rapidly approaching alternative to current methods. 展开更多
关键词 PERIPHERAL NERVE INTERFACES optogenetics OPTICAL neural interface OPTICAL PERIPHERAL NERVE interface GCaMP ArcLight adenoassociated VIRAL vector lentiviral VECTORS VIRAL VECTORS implantable microscopy
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Effect of exogenous spastin combined with polyethylene glycol on sciatic nerve injury 预览
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作者 Yao-Fa Lin Zheng Xie +3 位作者 Jun Zhou Hui-Hao Chen Wan-Wan Shao Hao-Dong Lin 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第7期1271-1279,共9页
Polyethylene glycol can connect the distal and proximal ends of an injured nerve at the cellular level through axonal fusion to avoid Wallerian degeneration of the injured distal nerve and promote peripheral nerve reg... Polyethylene glycol can connect the distal and proximal ends of an injured nerve at the cellular level through axonal fusion to avoid Wallerian degeneration of the injured distal nerve and promote peripheral nerve regeneration.However,this method can only prevent Wallerian degeneration in 10%of axons because the cytoskeleton is not repaired in a timely fashion.Reconstruction of the cytoskeletal trunk and microtubule network has been suggested to be the key for improving the efficiency of axonal fusion.As a microtubule-severing protein,spastin has been used to enhance cytoskeletal reconstruction.Therefore,we hypothesized that spastin combined with polyethylene glycol can more effectively promote peripheral nerve regeneration.A total of 120 male Sprague-Dawley rats were randomly divided into sham,suture,polyethylene glycol,and polyethylene glycol+spastin groups.In suture group rats,only traditional nerve anastomosis of the end-to-end suture was performed after transection of the sciatic nerve.In polyethylene glycol and polyethylene glycol+spastin groups,50μL of polyethylene glycol or 25μL of polyethylene glycol+25μL of spastin,respectively,were injected immediately under the epineurium of the distal suture.Sensory fiber regeneration distance,which was used to assess early nerve regeneration at 1 week after surgery,was shortest in the suture group,followed by polyethylene glycol group and greatest in the polyethylene glycol+spastin group.Behavioral assessment of motor function recovery in rats showed that limb function was restored in polyethylene glycol and polyethylene glycol+spastin groups at 8 weeks after surgery.At 1,2,4 and 8 weeks after surgery,sciatic functional index values and percentages of gastrocnemius muscle wet weight were highest in the sham group,followed by polyethylene glycol+spastin and polyethylene glycol groups,and lowest in the suture group.Masson staining was utilized to assess the morphology of muscle tissue.Morphological changes in skeletal muscle were detectable in suture,polyethylene g 展开更多
关键词 NERVE REGENERATION PERIPHERAL NERVES Wallerian degeneration polyethylene glycol axonal fusion SPASTIN PERIPHERAL NERVE injuries MASSON staining microtubule neural REGENERATION
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Differential gene and protein expression between rat tibial nerve and common peroneal nerve during Wallerian degeneration 预览
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作者 Yao-Fa Lin Zheng Xie +2 位作者 Jun Zhou Gang Yin Hao-Dong Lin 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第12期2183-2191,共9页
Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this i... Wallerian degeneration and nerve regeneration after injury are complex processes involving many genes, proteins and cytokines. After different peripheral nerve injuries the regeneration rate can differ. Whether this is caused by differential expression of genes and proteins during Wallerian degeneration remains unclear. The right tibial nerve and the common peroneal nerve of the same rat were exposed and completely cut through and then sutured in the same horizontal plane. On days 1, 7, 14, and 21 after surgery, 1–2 cm of nerve tissue distal to the suture site was dissected out from the tibial and common peroneal nerves. The differences in gene and protein expression during Wallerian degeneration of the injured nerves were then studied by RNA sequencing and proteomic techniques. In the tibial and common peroneal nerves, there were 1718, 1374, 1187, and 2195 differentially expressed genes, and 477, 447, 619, and 495 differentially expressed proteins on days 1, 7, 14, and 21 after surgery, respectively. Forty-seven pathways were activated during Wallerian degeneration. Three genes showing significant differential expression by RNA sequencing (Hoxd4, Lpcat4 and Tbx1) were assayed by real-time quantitative polymerase chain reaction. RNA sequencing and real-time quantitative polymerase chain reaction results were consistent. Our findings showed that expression of genes and proteins in injured tibial and the common peroneal nerves were significantly different during Wallerian degeneration at different time points. This suggests that the biological processes during Wallerian degeneration are different in different peripheral nerves after injury. The procedure was approved by the Animal Experimental Ethics Committee of the Second Military Medical University, China (approval No. CZ20160218) on February 18, 2016. 展开更多
关键词 NERVE REGENERATION PERIPHERAL NERVES PERIPHERAL NERVE injuries Wallerian degeneration TIBIAL NERVE common PERONEAL NERVE RNA sequencing proteomic real-time quantitative polymerase chain reaction neural REGENERATION
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Simultaneous enhancement of efficiency and stability of OLEDs with thermally activated delayed fluorescence materials by modifying carbazoles with peripheral groups
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作者 Yunge Zhang Dongdong Zhang +2 位作者 Taiju Tsuboi Yong Qiu Lian Duan 《中国科学:化学英文版》 SCIE EI CAS CSCD 2019年第3期393-402,共10页
Albeit their high efficiencies, the operational stability of the organic light emitting diodes(OLEDs) based on thermally activated delayed fluorescence(TADF) emitters is still far from satisfaction, and few strategies... Albeit their high efficiencies, the operational stability of the organic light emitting diodes(OLEDs) based on thermally activated delayed fluorescence(TADF) emitters is still far from satisfaction, and few strategies have been proposed to improve their stability. Here, we show that by modifying the carbazole unit, one of the most commonly used donors in TADF emitters, with peripheral groups, both the device efficiency and operational stability can be greatly improved. Awell-known TADF molecule-4,5-di(9H-carbazol-9-yl)phthalonitrile(2CzPN) was chosen as the prototype and modified by introducing peripheral tert-butyl and phenyl groups to the 3,6-positions of the carbazole(named 2tBuCzPN and 2PhCzPN, respectively). The introduced groups not only improve the compounds’ electrochemical stabilities referred to the cyclic voltammetry multi-sweep results, but also promote their photoluminescence quantum yields. Furthermore, reduced singlet-triplet energy gaps are observed, leading to the shortened exciton lifetimes which are benefit to suppress the exciton annihilations. Besides, the steric hindrance of introduced phenyl groups can partly restrain the concentration quenching of the TADF emitter. Consequently, OLEDs based on 2tBuCzPN and 2PhCzPN achieved improved maximum external quantum efficiencies(EQEs) of 17.0% and 14.0%, respectively(compared to 8.5% for 2CzPN). Meanwhile, 2PhCzPN based OLED showed reduced roll-off characteristics and a longer lifetime of 7.8 times higher than that of 2CzPN, testifying the effectiveness of subtle modification of the unstable moieties in simultaneous enhancement of efficiency and stability of OLEDs based on TADF emitters. 展开更多
关键词 TADF OLED STABILITY CARBAZOLE PERIPHERAL group
MicroRNAs in blood and cerebrospinal fluid as diagnostic biomarkers of multiple sclerosis and to monitor disease progression 预览
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作者 Bridget Martinez Philip V.Peplow 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期606-619,共14页
Multiple sclerosis is a chronic autoimmune disease of the central nervous system.It is the main cause of non-traumatic neurological disability in young adults.Multiple sclerosis mostly affects people aged 20–50 years... Multiple sclerosis is a chronic autoimmune disease of the central nervous system.It is the main cause of non-traumatic neurological disability in young adults.Multiple sclerosis mostly affects people aged 20–50 years;however,it can occur in young children and much older adults.Factors identified in the distribution of MS include age,gender,genetics,environment,and ethnic background.Multiple sclerosis is usually associated with progressive degrees of disability.The disease involves demyelination of axons of the central nervous system and causes brain and spinal cord neuronal loss and atrophy.Diagnosing multiple sclerosis is based on a patient’s medical history including symptoms,physical examination,and various tests such as magnetic resonance imaging,cerebrospinal fluid and blood tests,and electrophysiology.The disease course of multiple sclerosis is not well correlated with the biomarkers presently used in clinical practice.Blood-derived biomarkers that can detect and distinguish the different phenotypes in multiple sclerosis may be advantageous in personalized treatment with disease-modifying drugs and to predict response to treatment.The studies reviewed have shown that the expression levels of a large number of miRNAs in peripheral blood,serum,exosomes isolated from serum,and cerebrospinal fluid are altered in multiple sclerosis and can distinguish the disease phenotypes from each other.Further studies are warranted to independently validate these findings so that individual or pairs of miRNAs in serum or cerebrospinal fluid can be used as potential diagnostic markers for adult and pediatric multiple sclerosis and for monitoring disease progression and response to therapy. 展开更多
关键词 clinically isolated syndrome CSF disease progression EXOSOMES humans microRNA multiple sclerosis peripheral blood PHENOTYPES serum
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Protective effect of hydrogen sulfide on oxidative stress-induced neurodegenerative diseases 预览
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作者 Rubaiya Tabassum Na Young Jeong Junyang Jung 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第2期232-241,共10页
Hydrogen sulfide is an antioxidant molecule that has a wide range of biological effects against oxidative stress. Balanced oxidative stress is also vital for maintaining cellular function in biological system, where r... Hydrogen sulfide is an antioxidant molecule that has a wide range of biological effects against oxidative stress. Balanced oxidative stress is also vital for maintaining cellular function in biological system, where reactive oxygen species are the main source of oxidative stress. When the normal redox balance is disturbed, deoxyribonucleic acid, lipid, and protein molecules are oxidized under pathological conditions, like diabetes mellitus that leads to diabetic peripheral neuropathy. In diabetes mellitus-induced diabetic peripheral neuropathy, due to hyperglycemia, pancreatic beta cell(β cell) shows resistance to insulin secretion. As a consequence, glucose metabolism is disturbed in neuronal cells which are distracted from providing proper cell signaling pathway. Not only diabetic peripheral neuropathy but also other central damages occur in brain neuropathy. Neurological studies regarding type 1 diabetes mellitus patients with Alzheimer’s disease, Parkinson’s disease, and amyotrophic lateral sclerosis have shown changes in the central nervous system because high blood glucose levels(HbA1 c) appeared with poor cognitive function. Oxidative stress plays a role in inhibiting insulin signaling that is necessary for brain function. Hydrogen sulfide exhibits antioxidant effects against oxidative stress, where cystathionine β synthase, cystathionine γ lyase, and 3-mercaptopyruvate sulfurtransferase are the endogenous sources of hydrogen sulfide. This review is to explore the pathogenesis of diabetes mellitus-induced diabetic peripheral neuropathy and other neurological comorbid disorders under the oxidative stress condition and the anti-oxidative effects of hydrogen sulfide. 展开更多
关键词 Alzheimer's DISEASE amyotrophic lateral SCLEROSIS antioxidant diabetic peripheral NEUROPATHY DNA oxidation hydrogen SULFIDE mitochondrial dysfunction oxidative stress Parkinson's DISEASE reactive oxygen species
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Relationship between Maternal PBMC HBV cccDNA and HBV Serological Markers and its Effect on HBV Intrauterine Transmission 预览
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作者 WANG Dan Dan YI Lin Zhu +7 位作者 WU Li Na YANG Zhi Qing HAO Hai Yun SHI Xiao Hong WANG Bo FENG Shu Ying FENG Yong Liang WANG Su Ping 《生物医学与环境科学:英文版》 SCIE CAS CSCD 2019年第5期315-323,共9页
Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and... Objective To investigate the relationship between maternal peripheral blood mononuclear cells (PBMC) hepatitis B virus(HBV) covalenty closed circular deoxyribonucleic acid(cccDNA) and other HBV serological markers and its effects on HBV intrauterine transmission. Methods We enrolled 290 newborns and their hepatitis B surface antigen(HBsAg) positive mothers. HBV cccDNA in PBMC and HBV DNA in serum were detected by a real‐time PCR‐TaqM an probe while HBV serological markers were detected with an electrochemiluminescence immunoassay. Results There was a positive correlation between the levels of PBMC HBV cccD NA and serum HBV DNA and HBeA g(r = 0.436 and 0.403, P < 0.001). The detection rate of pattern A [‘HBsA g(+), HBeA g(+), and anti‐HBc(+)’] was significantly higher in the PBMC HBV cccD NA positive group than in the control group(χ^2 = 48.48, P < 0.001). There was a significant association between HBV intrauterine transmission and PBMC HBV cccD NA(χ^2 = 9.28, P = 0.002). In the presence of serum HBV DNA, HBeA g, and PBMC HBV cccD NA, the risk of HBV intrauterine transmission was three times higher(OR = 3.69, 95% CI: 1.30‐10.42) than that observed in their absence. The risk of HBV intrauterine transmission was the greatest(OR = 5.89, 95% CI: 2.35‐14.72) when both PBMC HBV cccD NA and pattern A were present. A Bayesian network model showed that maternal PBMC HBV cccD NA was directly related to HBV intrauterine transmission. Conclusion PBMC HBV cccDNA may be a direct risk factor for HBV intrauterine transmission. Our study suggests that serological markers could be combined with PBMC‐related markers in prenatal testing. 展开更多
关键词 Hepatitis B virus INTRAUTERINE transmission Peripheral blood MONONUCLEAR cells Covalently closed circular deoxyribonucleic acid SEROLOGICAL markers
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TrkA regulates the regenerative capacity of bone marrow stromal stem cells in nerve grafts 预览
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作者 Mei-Ge Zheng Wen-Yuan Sui +8 位作者 Zhen-Dan He Yan Liu Yu-Lin Huang Shu-Hua Mu Xin-Zhong Xu Ji-Sen Zhang Jun-Le Qu Jian Zhang Dong Wang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第10期1765-1771,共7页
We previously demonstrated that overexpression of tropomyosin receptor kinase A(TrkA)promotes the survival and Schwann celllike differentiation of bone marrow stromal stem cells in nerve grafts,thereby enhancing the r... We previously demonstrated that overexpression of tropomyosin receptor kinase A(TrkA)promotes the survival and Schwann celllike differentiation of bone marrow stromal stem cells in nerve grafts,thereby enhancing the regeneration and functional recovery of the peripheral nerve.In the present study,we investigated the molecular mechanisms underlying the neuroprotective effects of TrkA in bone marrow stromal stem cells seeded into nerve grafts.Bone marrow stromal stem cells from Sprague-Dawley rats were infected with recombinant lentivirus vector expressing rat TrkA,TrkA-shRNA or the respective control.The cells were then seeded into allogeneic rat acellular nerve allografts for bridging a 1-cm right sciatic nerve defect.Then,8 weeks after surgery,hematoxylin and eosin staining showed that compared with the control groups,the cells and fibers in the TrkA overexpressing group were more densely and uniformly arranged,whereas they were relatively sparse and arranged in a disordered manner in the TrkA-shRNA group.Western blot assay showed that compared with the control groups,the TrkA overexpressing group had higher expression of the myelin marker,myelin basic protein and the axonal marker neurofilament 200.The TrkA overexpressing group also had higher levels of various signaling molecules,including TrkA,pTrkA(Tyr490),extracellular signal-regulated kinases 1/2(Erkl/2),pErk1/2(Thr202/Tyr204),and the anti-apoptotic proteins Bcl-2 and Bcl-xL.In contrast,these proteins were downregulated,while the pro-apoptotic factors Bax and Bad were upregulated,in the TrkA-shRNA group.The levels of the TrkA effectors Akt and pAkt(Ser473)were not different among the groups.These results suggest that TrkA enhances the survival and regenerative capacity of bone marrow stromal stem cells through upregulation of the Erk/Bcl-2 pathway.All procedures were approved by the Animal Ethical and Welfare Committee of Shenzhen University,China in December 2014(approval No.AEWC-2014-001219). 展开更多
关键词 NERVE REGENERATION bone marrow stromal stem cells TROPOMYOSIN RECEPTOR kinase A RECEPTOR LENTIVIRAL vector shRNA extracellular SIGNAL-REGULATED protein kinases 1/2 Bcl-2 NERVE grafts peripheral NERVE REGENERATION survival neural REGENERATION
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Association between serum uric acid and large-nerve fiber dysfunction in type 2 diabetes:a cross-sectional study
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作者 Tian-Nan Jiang Yu-Feng Li +4 位作者 Li-Li Huo Qian Zhang Lian-Ying Wang Cui-Ling Zhao Li-Ge Liu 《中华医学杂志:英文版》 SCIE CAS CSCD 2019年第9期1015-1022,共8页
Background:Large-nerve fiber dysfunction,as assessed by vibration perception threshold (VPT) predicts risks of ulceration,amputation,and mortality in diabetes.Serum uric acid (UA) is closely associated with various me... Background:Large-nerve fiber dysfunction,as assessed by vibration perception threshold (VPT) predicts risks of ulceration,amputation,and mortality in diabetes.Serum uric acid (UA) is closely associated with various metabolic disorders,especially diabetes.Thus,we sought to investigate the clinical relevance of UA to large-nerve fiber dysfunction,among patients with type 2 diabetes (T2D).Methods:Medical records of consecutive patients with T2D who were admitted to Beijing Friendship Hospital Pinggu Campus between May 2014 and December 2016 were collected.Data for the 824 eligible patients included in the final analysis were extracted using a structured form.A VPT value ≥15 in either foot was defined as abnormal.We compared the clinical characteristics between patients with abnormal VPT and those with normal VPT (VPT value <15 in both feet) in the overall population and in gender subgroups.Logistic regression analysis was performed to explore the association of abnormal VPT with UA level.One-way analysis of variance was used to compare VPT values across four UA quartiles.Results:UA levels were significantly lower in T2D patients with abnormal VPT than in those with normal VPT (294.5 ± 84.0 vs.314.9 ± 92.8 μmol/L,P<0.01),especially among male patients (311.7 ± 85.2 vs.336.9 ± 89.6 μmol/L,P<0.01).From the logistic regression analysis,hyperuricemia (males >420 μmol/L;females >360 μmol/L) was associated with a reduced risk of abnormal VPT (odds ratio [OR],0.60;95% confidence interval [CI],0.39–0.91;P<0.05).This association was robust in male patients (OR,0.43;95% CI,0.24–0.76;P<0.01) but not in female patients (OR,0.92;95% CI,0.47–1.82;P = 0.816),even after adjustment for confounding factors.For the younger male subgroup (age <65 years),VPT values decreased as the UA level increased (P for trend = 0.002),but this trend was not significant in older male subgroup (age ≥65 years;P for trend = 0.400).Conclusions:Low serum UA levels showed a significant association with an increased risk of large-nerve fibe 展开更多
关键词 PERIPHERAL NEUROPATHY Uric ACID Type 2 DIABETES
Remnant neuromuscular junctions in denervated muscles contribute to functional recovery in delayed peripheral nerve repair 预览
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作者 Leyang Li Hiroyuki Yokoyama +5 位作者 Hidetoshi Kaburagi Takashi Hirai Kunikazu Tsuji Mitsuhiro Enomoto Yoshiaki Wakabayashi Atsushi Okawa 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期731-738,共8页
Schwann cell proliferation in peripheral nerve injury(PNI)enhances axonal regeneration compared to central nerve injury.However,even in PNI,long-term nerve damage without repair induces degeneration of neuromuscular j... Schwann cell proliferation in peripheral nerve injury(PNI)enhances axonal regeneration compared to central nerve injury.However,even in PNI,long-term nerve damage without repair induces degeneration of neuromuscular junctions(NMJs),and muscle atrophy results in irreversible dysfunction.The peripheral regeneration of motor axons depends on the duration of skeletal muscle denervation.To overcome this difficulty in nerve regeneration,detailed mechanisms should be determined for not only Schwann cells but also NMJ degeneration after PNI and regeneration after nerve repair.Here,we examined motor axon denervation in the tibialis anterior muscle after peroneal nerve transection in thy1-YFP mice and regeneration with nerve reconstruction using allografts.The number of NMJs in the tibialis anterior muscle was maintained up to 4 weeks and then decreased at 6 weeks after injury.In contrast,the number of Schwann cells showed a stepwise decline and then reached a plateau at 6 weeks after injury.For regeneration,we reconstructed the degenerated nerve with an allograft at 4 and 6 weeks after injury,and evaluated functional and histological outcomes for 10 to 12 weeks after grafting.A higher number of pretzel-shaped NMJs in the tibialis anterior muscle and better functional recovery were observed in mice with a 4-week delay in surgery than in those with a 6-week delay.Nerve repair within 4 weeks after PNI is necessary for successful recovery in mice.Prevention of synaptic acetylcholine receptor degeneration may play a key role in peripheral nerve regeneration.All animal experiments were approved by the Institutional Animal Care and Use Committee of Tokyo Medical and Dental University on 5 July 2017,30 March 2018,and 15 May 2019(A2017-311C,A2018-297A,and A2019-248A),respectively. 展开更多
关键词 AXON nerve allograft nerve regeneration NEURODEGENERATION neuromuscular junction peripheral nerve injury Schwann cell skeletal muscle
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一例早产儿下肢静脉机化物形成致PICC拔管困难的处理体会
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作者 冯小芳 张丽萍 +1 位作者 胡卫洁 孙彩霞 《中国实用护理杂志》 2019年第18期1413-1416,共4页
目的分析1例早产儿下肢静脉机化物形成致PICC拔管困难的原因和多学科合作的应用。方法通过对PICC拔管困难进行原因分析和处理,在多学科合作综合干预下拔管。结果患儿在数字减影血管造影下成功拔除PICC,但腘静脉内机化物残留,随访6个月... 目的分析1例早产儿下肢静脉机化物形成致PICC拔管困难的原因和多学科合作的应用。方法通过对PICC拔管困难进行原因分析和处理,在多学科合作综合干预下拔管。结果患儿在数字减影血管造影下成功拔除PICC,但腘静脉内机化物残留,随访6个月无机化物脱落、血管堵塞等不良后果。结论新生儿PICC拔管困难,可给予合适体位、湿热敷、局部按摩等方法,必要时B超确认有无血栓、机化物及纤维蛋白鞘形成等,应用多学科合作能提高最小创伤拔管成功率,针对拔管困难的原因采取有效的预防措施是PICC置管护理的重点。 展开更多
关键词 导管插入术 外周 早产儿 拔管困难 护理 多学科合作
Gene expression changes in dorsal root ganglia following peripheral nerve injury:roles in inflammation,cell death and nociception 预览
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作者 Sarah L. Martin Adam J. Reid +2 位作者 Alexei Verkhratsky Valerio Magnaghi Alessandro Faroni 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期939-947,共9页
Subsequent to a peripheral nerve injury,there are changes in gene expression within the dorsal root ganglia in response to the damage.This review selects factors which are well-known to be vital for inflammation,cell ... Subsequent to a peripheral nerve injury,there are changes in gene expression within the dorsal root ganglia in response to the damage.This review selects factors which are well-known to be vital for inflammation,cell death and nociception,and highlights how alterations in their gene expression within the dorsal root ganglia can affect functional recovery.The majority of studies used polymerase chain reaction within animal models to analyse the dynamic changes following peripheral nerve injuries.This review aims to highlight the factors at the gene expression level that impede functional recovery and are hence are potential targets for therapeutic approaches.Where possible the experimental model,specific time-points and cellular location of expression levels are reported. 展开更多
关键词 Gene expression polymerase chain reaction dorsal root GANGLIA INFLAMMATION NOCICEPTION cell death peripheral NERVE injury Schwann CELLS satellite GLIAL CELLS NERVE regeneration
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Management of pain in peripheral vascular disease with Jalauka Avacharana followed by Manjishtadi Kshara Basti-a case series 预览
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作者 Heramb Pundalik Hattikar Gururaja D Tantry 《TMR临床研究》 2019年第3期91-99,共9页
Peripheral vascular disease with increasing prevalence rate is one among many conditions where a dependable cure is still elusive.Peripheral arterial disease(PAD),of which is a common form that results from atheroscle... Peripheral vascular disease with increasing prevalence rate is one among many conditions where a dependable cure is still elusive.Peripheral arterial disease(PAD),of which is a common form that results from atherosclerosis of arteries leading to reduced blood flow to the extremities.Commonly affecting the lower extremity,PAD presents with pain during ambulation,which is known as“intermittent claudication”which later manifests into“rest pain”.Due to the cry of the dying nerves,the pain is so severe that even common analgesics fail to relieve it.It is observed that the pathogenesis and clinical manifestation of PAD has striking similarities with“gambhira vatarakta”which is characterised by burning sensation,severe pain,excessive sweating,thirst,suppuration and tenderness.Keeping this view in mind the patients diagnosed with vatarakta were selected for jalauka avacharana(Leech therapy)followed by manjishtadi kshara basti(Decoction enema prepared by adding several drugs).This protocol showed remarkable results in relieving pain and ultimately vyadhi upashaya. 展开更多
关键词 Peripheral Vascular Disease Intermittent claudication Rest Pain Vatarakta Jalauka Avacharana Manjishtadi Kshara Basti
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Study on gene expression patterns and functional pathways of peripheral blood monocytes reveals potential molecular mechanism of surgical treatment for periodontitis 预览
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作者 Jin-Ji Ma Hong-Mei Liu +2 位作者 Xiang-Hua Xu Li-Xin Guo Qing Lin 《世界临床病例杂志》 2019年第12期1383-1392,共10页
BACKGROUND Periodontitis is a chronic inflammation of periodontal supporting tissue caused by local factors. Periodontal surgery can change the gene expression of peripheral blood mononuclear cells. However, little is... BACKGROUND Periodontitis is a chronic inflammation of periodontal supporting tissue caused by local factors. Periodontal surgery can change the gene expression of peripheral blood mononuclear cells. However, little is known about the potential mechanism of surgical treatment for periodontitis. AIM To explore the potential molecular mechanism of surgical treatment for periodontitis. METHODS First, based on the expression profiles of genes related to surgical treatment for periodontitis, a set of expression disorder modules related to surgical treatment for periodontitis were obtained by enrichment analysis. Subsequently, based on crosstalk analysis, we proved that there was a significant crosstalk relationship between module 3 and module 5. Finally, based on predictive analysis of multidimensional regulators, we identified a series of regulatory factors, such as endogenous genes, non-coding RNAs (ncRNAs), and transcription factors, which have potential regulatory effects on periodontitis. RESULTS A total of 337 genes related to surgical treatment for periodontitis were obtained, and 3896 genes related to periodontitis were amplified. Eight expression modules of periodontitis were obtained, involving the aggregation of 2672 gene modules. These modules are mainly involved in G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger, and adenylate cyclasemodulating G-protein coupled receptor signaling pathway. In addition, eight endogenous genes (including EGF, RPS27A, and GNB3) were screened by network connectivity analysis. Finally, based on this set of potential dysfunction modules, 94 transcription factors (including NFKB1, SP1, and STAT3) and 1198 ncRNAs (including MALAT1, CRNDE, and ANCR) were revealed. These core regulators are thought to be involved in the potential molecular mechanism of periodontitis after surgical treatment. CONCLUSION Based on the results of this study, we can show biologists and pharmacists a new idea to reveal the potential molecular mechanism of surgic 展开更多
关键词 Peripheral blood MONONUCLEAR cells GENE EXPRESSION DYSREGULATION module POTENTIAL molecular mechanism GENE EXPRESSION pattern
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Professor Jiping ZHAO's experiences in the differentiation and treatment of peripheral facial paralysis with acupuncture
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作者 郑媛媛 王朋 赵吉平 《世界针灸杂志:英文版》 CSCD 2019年第1期76-79,共4页
Professor Jiping ZHAO has unique thoughts on the diagnosis and treatment of peripheral facial paralysis.She pays the great attention to clinical differentiation in treatment with acupuncture,especially meridian differ... Professor Jiping ZHAO has unique thoughts on the diagnosis and treatment of peripheral facial paralysis.She pays the great attention to clinical differentiation in treatment with acupuncture,especially meridian differentiation.She believes that the differentiation of lesion is core and the early judgment is critical for the better therapeutic effects on in tractable facial paralysis.The local acupoints are selected in the guide of the theory of meridian sinew and stimulated on the base of three-needling theory.The distal acupoints are decided in light of the syndrome differentiation,of which,Hegu(合谷LI 4),Zusanli(足三里ST 36)and Taichong(太冲LR 3)are the basic acupoints.Additionally,Professor ZHAO lays the emphasis on the 5th branch of facial nerve(cervical branch)in diagnosis and treatment. 展开更多
关键词 Peripheral facial PARALYSIS Lesion MERIDIAN muscle region Three NEEDLING Experiences of FAMOUS PHYSICIAN
Relationship of visual impairment and peripheral artery disease with the occurrence of diabetic foot ulcers in Dr. Moewardi Hospital 预览
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作者 Okti Sri Purwanti Krisna Yetti Tuti Herawati 《护理前沿(英文)》 CAS 2019年第2期157-160,共4页
Objective: One of the diabetes mellitus (DM) complications is foot ulcers. Foot ulcers are influenced by many factors. DM puts patients at risk of injury because of visual impairment. Hyperglycemia can damage blood ve... Objective: One of the diabetes mellitus (DM) complications is foot ulcers. Foot ulcers are influenced by many factors. DM puts patients at risk of injury because of visual impairment. Hyperglycemia can damage blood vessels, which results in peripheral artery disease (PAD). The purpose of this study was to find the relationship of visual impairment and PAD with diabetic foot ulcers. Methods: This research used observational analytic study with case–control methods. It used purposive sampling in 34 DM patients with foot ulcers and 34 DM patients without foot ulcers. The research instruments were an interview guide to assess visual impairment, physical examination to assess PAD, and documentation study, which was used to know the incidence of diabetic ulcers. Results: The results showed that the respondents who experienced ulcers with visual impairment were 44.1% and the respondents who had foot ulcers with PAD were 73.5%. The results of statistical tests showed that there was no relationship between visual impairment and the incidence of ulcers (P = 0.166). The respondents with PAD will have a chance to suffer from foot ulcers 5.808 times higher than those who do not have PAD. Conclusions: There is no relationship between visual impairment and the incidence of diabetic foot ulcers in Dr. Moewardi Hospital, but there is relationship between PAD and the incidence of diabetic foot ulcers in Dr. Moewardi Hospital. Suggestions of this study are to do foot screening and educate about the importance of foot care to prevent the occurrence of diabetic ulcers. 展开更多
关键词 visual IMPAIRMENT peripheral ARTERY DISEASE foot ULCERS diabetes MELLITUS ARTERY DISEASE RETINOPATHY
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Animal models used to study direct peripheral nerve repair:a systematic review 预览
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作者 Francisco Javier Vela Guadalupe Martínez-Chacón +3 位作者 Alberto Ballestín JoséLuis Campos Francisco Miguel Sánchez-Margallo Elena Abellán 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第3期491-502,共12页
Objective:Peripheral nerve repair is required after traumatic injury.This common condition represents a major public health problem worldwide.Recovery after nerve repair depends on several factors,including the severi... Objective:Peripheral nerve repair is required after traumatic injury.This common condition represents a major public health problem worldwide.Recovery after nerve repair depends on several factors,including the severity of the injury,the nerve involved,and the surgeon’s technical skills.Despite the precise microsurgical repair of nerve lesions,adequate functional recovery is not always achieved and,therefore,the regeneration process and surgical techniques are still being studied.Pre-clinical animal models are essential for this research and,for this reason,the focus of the present systematic review(according to the PRISMA statement)was to analyze the different animal models used in pre-clinical peripheral nerve repair studies.Data sources:Original articles,published in English from 2000 to 2018,were collected using the Web of Science,Scopus,and PubMed databases.Data selection:Only preclinical trials on direct nerve repair were included in this review.The articles were evaluated by the first two authors,in accordance with predefined data fields.Outcome measures:The primary outcomes included functional motor abilities,daily activity and regeneration rate.Secondary outcomes included coaptation technique and animal model.Results:This review yielded 267 articles,of which,after completion of the screening,49 studies were analyzed.There were 1425 animals in those 49 studies,being rats,mice,guinea pigs,rabbits,cats and dogs the different pre-clinical models.The nerves used were classified into three groups:head and neck(11),forelimb(8)and hindlimb(30).The techniques used to perform the coaptation were:microsuture(46),glue(12),laser(8)and mechanical(2).The follow-up examinations were histology(43),electrophysiological analysis(24)and behavioral observation(22).Conclusion:The most widely used animal model in the study of peripheral nerve repair is the rat.Other animal models are also used but the cost-benefit of the rat model provides several strengths over the others.Suture techniques are currently the first option for 展开更多
关键词 NERVE MICROSURGERY peripheral nerve regeneration repair reconstruction direct nerve repair animal model coaptation PRISMA systematic review
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云南澜沧老厂铅锌矿外围云山村一带地质特征及土壤Pb高值异常形成原因 预览
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作者 樊金虎 孙立秋 王鹏 《吉林地质》 2019年第1期49-54,共6页
老厂铅锌银多金属矿床位于云南省澜沧县境内,它是昌宁-孟连成矿带的南段唯一大型铅锌矿床,至今已有600余年的开采历史。但矿山企业目前面临资源枯竭的严峻形势。近年来,由于危机矿山接替资源项目的实施,矿区深部又发现细脉-浸染状钼(铜... 老厂铅锌银多金属矿床位于云南省澜沧县境内,它是昌宁-孟连成矿带的南段唯一大型铅锌矿床,至今已有600余年的开采历史。但矿山企业目前面临资源枯竭的严峻形势。近年来,由于危机矿山接替资源项目的实施,矿区深部又发现细脉-浸染状钼(铜)矿化。在目标指向深部的同时多家单位对老厂外围展开了找矿潜力评价工作,经局部土壤测量工作于矿区东侧的云山村一带发现多处Pb元素高值异常,多点异常值超过10 000 ppm。本文从多处搜集证据对Pb元素高值异常的特征及形成原因加以分析,进一步缩小找矿靶区,指导找矿方向。 展开更多
关键词 老厂铅锌银矿 外围 土壤测量 Pb异常特征 形成原因
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