目的了解惠州地区育龄期妇女无乳链球菌(Streptococcus agalactiae,S.agalactiae),又称B群链球菌(Group B Streptococcus,GBS)的检出情况,为惠州地区是否在孕晚期妇女中开展GBS筛查提供依据。方法收集2015年1月-2018年12月惠州市妇幼保...目的了解惠州地区育龄期妇女无乳链球菌(Streptococcus agalactiae,S.agalactiae),又称B群链球菌(Group B Streptococcus,GBS)的检出情况,为惠州地区是否在孕晚期妇女中开展GBS筛查提供依据。方法收集2015年1月-2018年12月惠州市妇幼保健计划生育服务中心就诊患者送检各类标本的细菌培养鉴定结果,分析GBS的检出情况及分布状况。结果2015年1月-2018年12月期间送检的48228份细菌培养鉴定标本中,共分离无乳链球菌525株,其中育龄期妇女标本中检出487株,主要分离自宫颈分泌物(88.91%)、阴道分泌物(6.98%)及羊水标本(2.05%);三类标本经细菌培养鉴定检出的病原菌中,GBS占比分别达20.16%、10.73%、29.41%,均位于前三位;2015-2018年间惠州地区育龄期妇女生殖系统标本中GBS的总检出率为3.9%,各年度间GBS的检出率差异无统计学意义(P>0.05);GBS阳性者主要集中于21~30岁、31~40岁两个年龄段,分别占65.41%、28.51%,但GBS引起育龄期妇女生殖系统感染的机率不存在年龄差异;生殖系统标本GBS的检出率分别为:宫颈分泌物3.81%、阴道分泌物4.81%、羊水7.19%,经分析差异无统计学意义(P>0.05)。结论GBS是引起惠州地区育龄期妇女生殖系统感染的主要病原体之一,惠州地区GBS的检出率不因年度而改变,且GBS引起育龄期妇女生殖系统感染的机率不存在年龄差异,鉴于其对妊娠结局的特殊影响,做好本地区孕晚期妇女GBS的筛查十分必要。展开更多
Objective: In traditional medicine, the seeds of Thai Mucuna pruriens(T-MP) are used to treat male dysuria and are believed to enhance fertility. However, information pertaining to the toxicity of T-MP and its interac...Objective: In traditional medicine, the seeds of Thai Mucuna pruriens(T-MP) are used to treat male dysuria and are believed to enhance fertility. However, information pertaining to the toxicity of T-MP and its interaction with other properties is limited. This study was thus conducted to evaluate the antioxidant capacity and subacute toxicity of T-MP in the reproductive system.Methods: Total phenolic content and antioxidant capacity of T-MP seed extract were determined using total phenolic content, 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays.Male and female adult rats were treated orally with T-MP at a dosage of 150 or 300 mg/kg body weight for 14 consecutive days. Sex hormones and functional parameters in the liver and kidney were evaluated.Histopathology of all tissue was conducted using Masson’s trichrome staining. Sperm parameters, including concentration, morphology, acrosome reaction status and DNA damage, were also examined.Expression of tyrosine phosphorylated protein(Tyr Pho), androgen receptor and A-kinase-anchoring protein 4(AKAP4) were investigated using the Western blot technique.Results: T-MP seed extract contained phenolic compounds and exhibited high antioxidant capacity with no toxicity at the tested doses. It did not affect liver or kidney function parameters in the male rats, but increased estradiol, aspartate aminotransferase and alanine aminotransferase levels in the females.Additionally, it decreased serum progesterone and alkaline phosphatase levels in female rats. Serum and intratesticular testosterone levels were significantly lower in male rats that received a high dosage of T-MP. Histopathological changes were not observed in any tissue treated with T-MP. T-MP also significantly increased sperm concentration(but did not affect sperm parameters), and enhanced testicular Tyr Pho protein and androgen receptor and expression of AKAP4 in sperms.Conclusion: T-MP seed extract exhibited antioxidant capacity and was not harmful to reproductive tissues. It also ha展开更多
The potato/tomato psyllid Bactericera cockerelli causes serious damage to several solanaceous crops by direct feeding and vectoring Candidatus Liberibacter solanacearum,a bacterial pathogen.Electron beam(eBeam)irradia...The potato/tomato psyllid Bactericera cockerelli causes serious damage to several solanaceous crops by direct feeding and vectoring Candidatus Liberibacter solanacearum,a bacterial pathogen.Electron beam(eBeam)irradiation is an environmentally friendly,chemical-free alternative method that is increasing in use for disinfestation of insect pests.We hypothesize that this irradiation technology will have detrimental effects on potato psyllid and thus impede its disease vectoring.To this end,we explored the effects of eBeam treatment ranging from 50 to 500 Gy on survival,development and reproduction of this pest.Impact on psyllids was apparently dose-dependent.When irradiated at 350 Gy,eggs could not hatch,1st instar nymphs failed to emerge,and although a small portion of irradiated 5th instar nymphs survived,the emerged adults were mostly deformed.Abnormality in eclosed adults suggests harmful effects of eBeam on metamorphosis.Reproduction was seriously impaired when female psyllids were exposed to eBeam at the 5th instar nymphal or young adult stage,presumably due to inability to form oocytes.In addition,reciprocal crosses between irradiated and untreated psyllids indicated that female psyllids were more radiosensitive than males to eBeam.Taken together,these findings indicate that eBeam negatively impacted potato psyllid development and reproduction,which would inevitably compromise its disease transmission capacity.A dose of 350 Gy can be considered as a reference dose for effective control of potato psyllids.展开更多
文摘目的了解惠州地区育龄期妇女无乳链球菌(Streptococcus agalactiae,S.agalactiae),又称B群链球菌(Group B Streptococcus,GBS)的检出情况,为惠州地区是否在孕晚期妇女中开展GBS筛查提供依据。方法收集2015年1月-2018年12月惠州市妇幼保健计划生育服务中心就诊患者送检各类标本的细菌培养鉴定结果,分析GBS的检出情况及分布状况。结果2015年1月-2018年12月期间送检的48228份细菌培养鉴定标本中,共分离无乳链球菌525株,其中育龄期妇女标本中检出487株,主要分离自宫颈分泌物(88.91%)、阴道分泌物(6.98%)及羊水标本(2.05%);三类标本经细菌培养鉴定检出的病原菌中,GBS占比分别达20.16%、10.73%、29.41%,均位于前三位;2015-2018年间惠州地区育龄期妇女生殖系统标本中GBS的总检出率为3.9%,各年度间GBS的检出率差异无统计学意义(P>0.05);GBS阳性者主要集中于21~30岁、31~40岁两个年龄段,分别占65.41%、28.51%,但GBS引起育龄期妇女生殖系统感染的机率不存在年龄差异;生殖系统标本GBS的检出率分别为:宫颈分泌物3.81%、阴道分泌物4.81%、羊水7.19%,经分析差异无统计学意义(P>0.05)。结论GBS是引起惠州地区育龄期妇女生殖系统感染的主要病原体之一,惠州地区GBS的检出率不因年度而改变,且GBS引起育龄期妇女生殖系统感染的机率不存在年龄差异,鉴于其对妊娠结局的特殊影响,做好本地区孕晚期妇女GBS的筛查十分必要。
基金The National Research Council of Thailand and Khon Kaen University (research project number: 6100018) provided grants for T-MP preparation and animal experimentThe Research Institute for Human High Performance and Health Promotion, Khon Kaen University provided apparatus and chemicals for antioxidant assays.
文摘Objective: In traditional medicine, the seeds of Thai Mucuna pruriens(T-MP) are used to treat male dysuria and are believed to enhance fertility. However, information pertaining to the toxicity of T-MP and its interaction with other properties is limited. This study was thus conducted to evaluate the antioxidant capacity and subacute toxicity of T-MP in the reproductive system.Methods: Total phenolic content and antioxidant capacity of T-MP seed extract were determined using total phenolic content, 2,2-diphenyl-1-picrylhydrazyl and ferric reducing antioxidant power assays.Male and female adult rats were treated orally with T-MP at a dosage of 150 or 300 mg/kg body weight for 14 consecutive days. Sex hormones and functional parameters in the liver and kidney were evaluated.Histopathology of all tissue was conducted using Masson’s trichrome staining. Sperm parameters, including concentration, morphology, acrosome reaction status and DNA damage, were also examined.Expression of tyrosine phosphorylated protein(Tyr Pho), androgen receptor and A-kinase-anchoring protein 4(AKAP4) were investigated using the Western blot technique.Results: T-MP seed extract contained phenolic compounds and exhibited high antioxidant capacity with no toxicity at the tested doses. It did not affect liver or kidney function parameters in the male rats, but increased estradiol, aspartate aminotransferase and alanine aminotransferase levels in the females.Additionally, it decreased serum progesterone and alkaline phosphatase levels in female rats. Serum and intratesticular testosterone levels were significantly lower in male rats that received a high dosage of T-MP. Histopathological changes were not observed in any tissue treated with T-MP. T-MP also significantly increased sperm concentration(but did not affect sperm parameters), and enhanced testicular Tyr Pho protein and androgen receptor and expression of AKAP4 in sperms.Conclusion: T-MP seed extract exhibited antioxidant capacity and was not harmful to reproductive tissues. It also ha
基金the AgriLife Research Insect Vector Diseases Grant Programthe USDA-AFRI grant(2014-67013-21781)+1 种基金USDA-APHIS(AP18PPQS&T00C235)China Scholarship Council.
文摘The potato/tomato psyllid Bactericera cockerelli causes serious damage to several solanaceous crops by direct feeding and vectoring Candidatus Liberibacter solanacearum,a bacterial pathogen.Electron beam(eBeam)irradiation is an environmentally friendly,chemical-free alternative method that is increasing in use for disinfestation of insect pests.We hypothesize that this irradiation technology will have detrimental effects on potato psyllid and thus impede its disease vectoring.To this end,we explored the effects of eBeam treatment ranging from 50 to 500 Gy on survival,development and reproduction of this pest.Impact on psyllids was apparently dose-dependent.When irradiated at 350 Gy,eggs could not hatch,1st instar nymphs failed to emerge,and although a small portion of irradiated 5th instar nymphs survived,the emerged adults were mostly deformed.Abnormality in eclosed adults suggests harmful effects of eBeam on metamorphosis.Reproduction was seriously impaired when female psyllids were exposed to eBeam at the 5th instar nymphal or young adult stage,presumably due to inability to form oocytes.In addition,reciprocal crosses between irradiated and untreated psyllids indicated that female psyllids were more radiosensitive than males to eBeam.Taken together,these findings indicate that eBeam negatively impacted potato psyllid development and reproduction,which would inevitably compromise its disease transmission capacity.A dose of 350 Gy can be considered as a reference dose for effective control of potato psyllids.