期刊文献+
共找到2篇文章
< 1 >
每页显示 20 50 100
Antiepileptic geissoschizine methyl ether is an inhibitor of multiple neuronal channels 认领
1
作者 Zhu-qing Xie Xiao-ting Tian +5 位作者 Yue-ming Zheng Li Zhan Xue-qin Chen Xiao-ming Xin Cheng-gang Huang Zhao-bing Gao 《中国药理学报:英文版》 SCIE CAS CSCD 2020年第5期629-637,共9页
Geissoschizine methyl ether(GM)is an indole alkaloid isolated from Uncaria rhynchophyll(UR)that has been used for the treatment of epilepsy in traditional Chinese medicine.An early study in a glutamate-induced mouse s... Geissoschizine methyl ether(GM)is an indole alkaloid isolated from Uncaria rhynchophyll(UR)that has been used for the treatment of epilepsy in traditional Chinese medicine.An early study in a glutamate-induced mouse seizure model demonstrated that GM was one of the active ingredients of UR.In this study,electrophysiological technique was used to explore the mechanism underlying the antiepileptic activity of GM.We first showed that GM(1−30μmol/L)dose-dependently suppressed the spontaneous firing and prolonged the action potential duration in cultured mouse and rat hippocampal neurons.Given the pivotal roles of ion channels in regulating neuronal excitability,we then examined the effects of GM on both voltage-gated and ligand-gated channels in rat hippocampal neurons.We found that GM is an inhibitor of multiple neuronal channels:GM potently inhibited the voltage-gated sodium(NaV),calcium(CaV),and delayed rectifier potassium(IK)currents,and the ligand-gated nicotinic acetylcholine(nACh)currents with IC50 values in the range of 1.3−13.3μmol/L.In contrast,GM had little effect on the voltage-gated transient outward potassium currents(IA)and four types of ligand-gated channels(γ-amino butyric acid(GABA),N-methyl-D-aspartate(NMDA),α-amino-3-hydroxy-5-methylisoxazole-4-propionate/kainite(AMPA/KA receptors)).The in vivo antiepileptic activity of GM was validated in two electricity-induced seizure models.In the maximal electroshock(MES)-induced mouse seizure model,oral administration of GM(50−100 mg/kg)dose-dependently suppressed generalized tonic-clonic seizures.In 6-Hz-induced mouse seizure model,oral administration of GM(100 mg/kg)reduced treatment-resistant seizures.Thus,we conclude that GM is a promising antiepileptic candidate that inhibits multiple neuronal channels. 展开更多
关键词 geissoschizine methyl ether Uncaria rhynchophyll antiepileptic drug hippocampal neurons action potential nicotinic acetylcholine receptors voltage-gated ion channels mouse seizure model
光照强度对钩藤生长及有效成分含量的影响 认领
2
作者 王健健 姬拉拉 +3 位作者 邓小红 吕立堂 喻理飞 关萍 《中国中药杂志》 CAS CSCD 北大核心 2019年第23期5118-5123,共6页
该文旨在研究不同光照强度对钩藤生长、生物量积累与分配、叶绿素含量及有效成分的影响,探索钩藤幼苗人工栽培适宜光强条件。以三年生钩藤幼苗为试验材料,采用不同层数黑色遮阳网,设置4个光强水平(100%,70%~75%,30%~35%,5%~10%)进行遮... 该文旨在研究不同光照强度对钩藤生长、生物量积累与分配、叶绿素含量及有效成分的影响,探索钩藤幼苗人工栽培适宜光强条件。以三年生钩藤幼苗为试验材料,采用不同层数黑色遮阳网,设置4个光强水平(100%,70%~75%,30%~35%,5%~10%)进行遮阴处理。结果表明,随光强减弱,钩藤幼苗株高、基径、(根、茎、叶、钩)生物量均呈"增大-减小"的变化趋势,70%~75%光强下,钩藤幼苗株高、基径、(根、茎、叶、钩)生物量显著高于其他处理(P<0. 05);5%~10%光强下,株高、基径、(根、钩)生物量显著低于其他处理(P<0. 05);随光强减弱,钩藤幼苗叶绿素含量逐渐增多,100%光照下叶绿素含量最小,5%~10%光强下叶绿素含量最高;钩藤幼苗不同器官钩藤碱与异钩藤碱含量随光照强度降低呈不同的变化规律,光强30%~35%下钩藤钩中钩藤碱与异钩藤碱及茎中钩藤碱含量最高,光强5%~10%下钩藤叶片中钩藤碱与异钩藤碱及茎中异钩藤碱含量最高。综上,光强70%~75%适宜幼苗生长和生物量积累;光强30%~35%适宜钩藤幼苗有效成分的积累。 展开更多
关键词 钩藤 光照强度 生长 有效成分
上一页 1 下一页 到第
使用帮助 返回顶部 意见反馈