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代谢组学在衰老研究中的进展 预览
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作者 刘传铃 王佳贺 《中国医药导报》 CAS 2019年第1期57-60,共4页
代谢组学是近年来快速发展的“组学”,其优势在于高敏感度、高精确度、费用较低,具有广阔的发展前景。细胞衰老是随着生物体年龄增大,细胞增殖、分化能力和生理功能逐步退变的过程。代谢组学技术可以动态监测机体随细胞衰老代谢产物的... 代谢组学是近年来快速发展的“组学”,其优势在于高敏感度、高精确度、费用较低,具有广阔的发展前景。细胞衰老是随着生物体年龄增大,细胞增殖、分化能力和生理功能逐步退变的过程。代谢组学技术可以动态监测机体随细胞衰老代谢产物的改变。代谢组学在衰老研究中具有重要意义,本文综述了代谢组学在衰老研究中的进展。这些研究发展加深了人们对于细胞衰老机制及过程的了解,也为衰老相关性疾病的研究提供了新的研究方法及思路。 展开更多
关键词 代谢组学 衰老 细胞衰老 衰老相关性疾病
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Effect of 2LMISEN<sup>®</sup>on Long-Term Hippocampal Neurons Culture as a Screening Senescent Cells Model: p16<sup>INK4A</sup>and Caspase 3 Quantification 预览
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作者 Nicoletta Libera Lilli Delphine Révy +2 位作者 Christelle Volteau Sandra Robelet Béatrice Lejeune 《老年问题研究(英文)》 2019年第6期155-164,共10页
The aim of the present study was to investigate the effect of one capsule of the micro-immunotherapy medicine (MIM) 2LMISEN&reg;compared to vehicle, in a neuronal aging model. Senescence and apoptosis of hippocamp... The aim of the present study was to investigate the effect of one capsule of the micro-immunotherapy medicine (MIM) 2LMISEN&reg;compared to vehicle, in a neuronal aging model. Senescence and apoptosis of hippocampal neurons were evaluated by measuring p16INK4a and caspase 3 levels, respectively. The data presented is a single observation. Mice hippocampal neuron cultures were treated with MIM (11 mM) or vehicle (11 mM) from 22 days in vitro (DIV) until 27 DIV. After incubation, hippocampal neuron cultures were fixed at 15 (control condition), 22, 25 and 27 DIV and then incubated with primary antibodies p16INK4a, MAP2 and Caspase 3. Quantification of p16INK4a and Caspase 3-positive neurons was done using Developer software. We found that vehicle had no effect on p16INK4a expression, whereas MIM was able to decrease p16INK4a levels at 22, 25 and 27 DIV in a statistically significant manner. The MIM had no significative effect on Caspase 3 expression. Our preliminary results showed that the MIM capsule significantly reduced neuronal senescence and not apoptosis. 展开更多
关键词 Neurons SENESCENCE Micro-Immunotherapy Low DOSE
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Hepatic senescence,the good and the bad 预览
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作者 Nazmul Huda Gang Liu +3 位作者 Honghai Hong Shengmin Yan Bilon Khambu Xiao-Ming Yin 《世界胃肠病学杂志:英文版》 SCIE CAS 2019年第34期5069-5081,共13页
Gradual alterations of cell’s physiology and functions due to age or exposure to various stresses lead to the conversion of normal cells to senescent cells.Once becoming senescent,the cell stops dividing permanently ... Gradual alterations of cell’s physiology and functions due to age or exposure to various stresses lead to the conversion of normal cells to senescent cells.Once becoming senescent,the cell stops dividing permanently but remains metabolically active.Cellular senescence does not have a single marker but is characterized mainly by a combination of multiple markers,such as,morphological changes,expression of cell cycle inhibitors,senescence associatedβ-galactosidase activity,and changes in nuclear membrane.When cells in an organ become senescent,the entire organism can be affected.This may occur through the senescence-associated secretory phenotype(SASP).SASP may exert beneficial or harmful effects on the microenvironment of tissues.Research on senescence has become a very exciting field in cell biology since the link between age-related diseases,including cancer,and senescence has been established.The loss of regenerative and homeostatic capacity of the liver over the age is somehow connected to cellular senescence.The major contributors of senescence properties in the liver are hepatocytes and cholangiocytes.Senescent cells in the liver have been implicated in the etiology of chronic liver diseases including cirrhosis and hepatocellular carcinoma and in the interference of liver regeneration.This review summarizes recently reported findings in the understanding of the molecular mechanisms of senescence and its relationship with liver diseases. 展开更多
关键词 SENESCENCE SENESCENCE associated SECRETORY phenotype HEPATOCYTE CHOLANGIOCYTE HEPATIC stellate CELL CELL cycle arrest DNA damage
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Erythrocyte Senescent Markers by Flow Cytometry 预览
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作者 María Alejandra Ensinck Melina Eliana Luján Brajovich +2 位作者 Silvia Estela García Borrás Carlos Miguel Cotorruelo Claudia Silvia Biondi 《血液病期刊(英文)》 2019年第3期47-59,共13页
Background: Mature red blood cells lack protein synthesis and are unable to restore inactivated enzymes, damaged cytoskeleton and membrane proteins. An oxidation breakdown of band 3 is probably part of the mechanism l... Background: Mature red blood cells lack protein synthesis and are unable to restore inactivated enzymes, damaged cytoskeleton and membrane proteins. An oxidation breakdown of band 3 is probably part of the mechanism leading to the generation of a senescent cell antigen. This specific signal serves for the clearance of RBCs by inducing the binding of autologous IgG and C3, leading to phagocytosis. In addition, phosphatidilserin molecules appear in the outer membrane and the CD47 expression diminishes. Methods: Erythrocytes of different ages from whole blood were studied by flow cytometry analysing light scatter proprieties, binding of autologous IgG, C3 complement deposits, externalization of phosphatidylserine and CD47 expression. Dot-plot analysis based on forward scatter versus side scatter parameters showed two RBCs populations of different sizes and density. RBCs were further incubated with Alexa 488 IgG, APC-anti-C3, PE-annexin-V and PE-CD47. The comparison of the values obtained for the different variables studied in SeRBC and YRBC populations was carried out by the Student t-test for matched samples or by the Wilcoxon test (after verification of the normality assumption). Results: The percentage of IgG and C3 positive cells was significantly higher in senescent red blood cells population. The fraction of annexin-V positive RBCs was also larger in SeRBCs while the CD47 expression was lower in this population. Conclusions: These results indicate that flow cytometry allow differenciation of erythrocytes populations of different ages, turning this tool into an useful alternative option to study erythrocyte aging process. These findings will contribute to a better understanding of the process and mechanisms involved in erythrocyte senescence process. 展开更多
关键词 ERYTHROCYTE SENESCENCE Flow CYTOMETRY
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Enhancing survival,engraftment,and osteogenic potential of mesenchymal stem cells 预览
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作者 Daniel García-Sánchez Darío Fernández +1 位作者 JoséC Rodríguez-Rey Flor M Pérez-Campo 《世界干细胞杂志:英文版(电子版)》 2019年第10期748-763,共16页
Mesenchymal stem cells(MSCs)are promising candidates for bone regeneration therapies due to their plasticity and easiness of sourcing.MSC-based treatments are generally considered a safe procedure,however,the long-ter... Mesenchymal stem cells(MSCs)are promising candidates for bone regeneration therapies due to their plasticity and easiness of sourcing.MSC-based treatments are generally considered a safe procedure,however,the long-term results obtained up to now are far from satisfactory.The main causes of these therapeutic limitations are inefficient homing,engraftment,and osteogenic differentiation.Many studies have proposed modifications to improve MSC engraftment and osteogenic differentiation of the transplanted cells.Several strategies are aimed to improve cell resistance to the hostile microenvironment found in the recipient tissue and increase cell survival after transplantation.These strategies could range from a simple modification of the culture conditions,known as cell-preconditioning,to the genetic modification of the cells to avoid cellular senescence.Many efforts have also been done in order to enhance the osteogenic potential of the transplanted cells and induce bone formation,mainly by the use of bioactive or biomimetic scaffolds,although alternative approaches will also be discussed.This review aims to summarize several of the most recent approaches,providing an up-to-date view of the main developments in MSCbased regenerative techniques. 展开更多
关键词 Mesenchymal stem cells Bone regeneration Hypoxia ANOIKIS PRECONDITIONING Bioactive scaffolds SENESCENCE ENGRAFTMENT HOMING OSTEOGENESIS
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Aging:A cell source limiting factor in tissue engineering 预览
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作者 Mohammadhossein Khorraminejad-Shirazi Mohammadreza Dorvash +3 位作者 Alireza Estedlal Amir Human Hoveidaei Mohsen Mazloomrezaei Pouria Mosaddeghi 《世界干细胞杂志:英文版(电子版)》 2019年第10期787-802,共16页
Tissue engineering has yet to reach its ideal goal,i.e.creating profitable off-theshelf tissues and organs,designing scaffolds and three-dimensional tissue architectures that can maintain the blood supply,proper bioma... Tissue engineering has yet to reach its ideal goal,i.e.creating profitable off-theshelf tissues and organs,designing scaffolds and three-dimensional tissue architectures that can maintain the blood supply,proper biomaterial selection,and identifying the most efficient cell source for use in cell therapy and tissue engineering.These are still the major challenges in this field.Regarding the identification of the most appropriate cell source,aging as a factor that affects both somatic and stem cells and limits their function and applications is a preventable and,at least to some extents,a reversible phenomenon.Here,we reviewed different stem cell types,namely embryonic stem cells,adult stem cells,induced pluripotent stem cells,and genetically modified stem cells,as well as their sources,i.e.autologous,allogeneic,and xenogeneic sources.Afterward,we approached aging by discussing the functional decline of aged stem cells and different intrinsic and extrinsic factors that are involved in stem cell aging including replicative senescence and Hayflick limit,autophagy,epigenetic changes,miRNAs,mTOR and AMPK pathways,and the role of mitochondria in stem cell senescence.Finally,various interventions for rejuvenation and geroprotection of stem cells are discussed.These interventions can be applied in cell therapy and tissue engineering methods to conquer aging as a limiting factor,both in original cell source and in the in vitro proliferated cells. 展开更多
关键词 AGING SENESCENCE REJUVENATION Geroprotection TISSUE engineering Stem CELL therapy
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不同浓度雌二醇对过氧化氢诱导的人脐静脉内皮细胞衰老模型的影响
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作者 林捷琪 阮云军 +3 位作者 杨儒于 王玉筵 苏双 吴赛珠 《中国临床解剖学杂志》 CSCD 北大核心 2019年第5期528-533,541共7页
目的 探究不同浓度的17β-雌二醇(17β-Estradiol,17β-E2)对过氧化氢(hydrogen peroxide,H2O2)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)衰老模型的影响。方法构建HUVECs衰老模型后将细胞分为空白组,衰... 目的 探究不同浓度的17β-雌二醇(17β-Estradiol,17β-E2)对过氧化氢(hydrogen peroxide,H2O2)诱导的人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)衰老模型的影响。方法构建HUVECs衰老模型后将细胞分为空白组,衰老组,17β-E2阴性对照组,低浓度、生理浓度及高浓度17β-E2组,CCK-8检测细胞活力;Western blot检测细胞衰老程度相关蛋白p-Rb、Rb、细胞自噬水平相关蛋白P62、LC3B以及衰老相关β半乳糖苷酶(SA-β-Gal)染色。结果 与空白组相比,衰老组p-Rb/Rb比值增加,细胞活力下降,SA-β-Gal染色阳性细胞数目多,与衰老组比较,高浓度和生理浓度17β-E2组p-Rb/Rb比值降低,SA-β-Gal染色阳性细胞数减少,P62表达量减少,LC3-Ⅱ/LC3-I蛋白表达升高,细胞活力增加。结论 17β-E2能通过上调自噬减轻H2O2诱导的HUVECs衰老,且与其浓度有关。 展开更多
关键词 自噬 血管内皮细胞 衰老 17Β-雌二醇
基于iTRAQ技术研究采后1-甲基环丙烯和乙烯利处理对茭白线粒体蛋白质组变化的影响 预览
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作者 罗海波 周涛 +5 位作者 孔晓雪 陶明煊 姜丽 王利斌 王韦华 郁志芳 《食品科学》 EI CAS CSCD 北大核心 2019年第1期216-225,共10页
为探讨茭白采后衰老的分子机制,应用同位素标记相对与绝对定量蛋白质组学技术研究了茭白常温贮藏期间线粒体蛋白质表达谱的变化及1-甲基环丙烯(1-methyleyelopropene,1-MCP)和乙烯利(ethylene,ET)处理对茭白线粒体蛋白质组变化的影响。... 为探讨茭白采后衰老的分子机制,应用同位素标记相对与绝对定量蛋白质组学技术研究了茭白常温贮藏期间线粒体蛋白质表达谱的变化及1-甲基环丙烯(1-methyleyelopropene,1-MCP)和乙烯利(ethylene,ET)处理对茭白线粒体蛋白质组变化的影响。结果表明:共鉴定到肽段数大于等于2的可信蛋白1908个,与贮藏0d相比,对照(CK)组、ET和1-MCP处理组茭白贮藏3d和6d后,共有315个蛋白表达量变化倍数在2.0倍以上且重复组数据统计学差异显著(P<0.05)。生物信息学分析显示代谢途径、次生代谢产物生物合成、氨基酸生物合成与代谢、核苷酸代谢、含碱基小分子代谢途径等可能与茭白采后衰老有关,三羧酸循环、氧化磷酸化、磷酸戊糖途径、C5支链二元酸代谢及氨基酸代谢途径可能在茭白采后衰老中发挥重要作用。这些差异表达蛋白的生物学功能分析表明,茭白采后碳水化合物水解加速,磷酸戊糖途径加强而糖酵解途径和氧化磷酸化减弱,导致能量合成减少,同时形成氧化胁迫,这可能激活Ca^2+/MAPKs、细胞色素c和茉莉酸等信号途径,造成初级代谢紊乱和次级代谢产物(如木质素)积累,从而促进细胞凋亡或细胞坏死,最终加速衰老。 展开更多
关键词 茭白 线粒体 同位素标记相对与绝对定量 生物信息学 衰老
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机体衰老对骨细胞力学响应的影响
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作者 杜静珂 于志锋 《医用生物力学》 CAS CSCD 北大核心 2019年第3期333-338,共6页
骨骼是一个动态变化的器官,骨细胞的形态、结构和功能随力学刺激大小、方向、形式的不同而发生变化。适当的力学刺激是维持骨形成和骨吸收动态平衡的关键。随着年龄的增加,骨组织衰老会引起包括骨组织微环境、骨细胞形态、骨细胞内信号... 骨骼是一个动态变化的器官,骨细胞的形态、结构和功能随力学刺激大小、方向、形式的不同而发生变化。适当的力学刺激是维持骨形成和骨吸收动态平衡的关键。随着年龄的增加,骨组织衰老会引起包括骨组织微环境、骨细胞形态、骨细胞内信号通路等在内的一系列变化,使骨骼力学响应能力减弱,进而引起骨质疏松等多种疾病。因此,研究衰老如何影响骨细胞的力学响应具有重要意义。重点讨论机体衰老对骨细胞力学响应的影响。 展开更多
关键词 力学响应 衰老 骨细胞 骨重建
硝普钠对生姜根茎抗氧化酶和苯丙烷途径的影响 预览
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作者 李雪 葛永红 +4 位作者 李灿婴 段斌 魏美林 唐琦 陈延儒 《食品工业科技》 CAS 北大核心 2019年第2期276-281,共6页
以生姜根茎为试材,研究采后不同浓度硝普钠溶液浸泡处理10 min对其呼吸强度和失重率的影响并筛选最佳浓度,同时分析最佳浓度处理后生姜根茎抗氧化酶活性及苯丙烷途径关键酶活性和产物含量的变化。结果表明,0.25 mmol/L硝普钠处理降低了... 以生姜根茎为试材,研究采后不同浓度硝普钠溶液浸泡处理10 min对其呼吸强度和失重率的影响并筛选最佳浓度,同时分析最佳浓度处理后生姜根茎抗氧化酶活性及苯丙烷途径关键酶活性和产物含量的变化。结果表明,0.25 mmol/L硝普钠处理降低了生姜根茎呼吸强度、抑制了失重率的升高,硝普钠处理还显著提高了生姜根茎过氧化氢含量、超氧化物歧化酶、过氧化物酶和抗坏血酸过氧化物酶的活性。此外,硝普钠处理还提高了苯丙氨酸解氨酶活性、显著提高了4-香豆酰CoA连接酶的活性,促进了木质素、总酚和类黄酮的积累。由此表明,硝普钠处理通过调节生姜根茎抗氧化酶活性和苯丙烷途径延缓其衰老。 展开更多
关键词 生姜根茎 硝普钠 衰老 呼吸强度
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能量状态在果蔬采后衰老中的作用及其调控研究进展 预览
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作者 李美玲 林育钊 +2 位作者 王慧 林毅雄 林河通 《食品科学》 EI CAS CSCD 北大核心 2019年第9期290-295,共6页
能量是采后果蔬维持正常生理代谢活动的基础。近年来的研究表明,能量亏缺是导致采后果蔬衰老的重要因素。本文从采后果蔬的呼吸代谢、活性氧和细胞膜完整性与能量状态的关系等方面阐述能量状态在果蔬采后衰老中的作用,并对采后果蔬能量... 能量是采后果蔬维持正常生理代谢活动的基础。近年来的研究表明,能量亏缺是导致采后果蔬衰老的重要因素。本文从采后果蔬的呼吸代谢、活性氧和细胞膜完整性与能量状态的关系等方面阐述能量状态在果蔬采后衰老中的作用,并对采后果蔬能量状态的调控措施以及调控机制等方面的研究进展进行综述。此外,从能量合成、转运、耗散和感知相关基因在转录和蛋白水平的表达与调控,采后果蔬衰老的起始因子,能量亏缺的应激反应等方面进行展望,旨在为从能量角度探究采后果蔬衰老的机制和研发果蔬采后保鲜新技术提供参考。 展开更多
关键词 果蔬 衰老 能量状态 能量亏缺 能量调控
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白藜芦醇对氧化应激环境中HUVECs细胞衰老、增殖、凋亡的影响及机制 预览
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作者 陈昌喆 宋晨曦 +3 位作者 李彬 张冬 张睿 窦克非 《中国老年学杂志》 CAS 北大核心 2019年第16期4027-4031,共5页
目的探讨白藜芦醇(RSV)在氧化应激环境中对人脐静脉内皮细胞(HUVECs)衰老、增殖及凋亡的影响,并对相关机制进行探索。方法利用β-半乳糖苷酶染色HUVECs衰老的细胞并记录阳性细胞数,观察过氧化氢(H2O2)单独处理以及联合RSV后,细胞衰老情... 目的探讨白藜芦醇(RSV)在氧化应激环境中对人脐静脉内皮细胞(HUVECs)衰老、增殖及凋亡的影响,并对相关机制进行探索。方法利用β-半乳糖苷酶染色HUVECs衰老的细胞并记录阳性细胞数,观察过氧化氢(H2O2)单独处理以及联合RSV后,细胞衰老情况的变化;采用长时程动态活细胞成像及分析系统检测H2O2单独处理以及联合RSV后细胞增殖能力的变化并绘制生长曲线;利用流式细胞技术检测H2O2单独及联合RSV对HUVECs细胞周期及细胞凋亡情况的影响;采用Western印迹检测相关蛋白表达情况。结果HUVECs经H2O2处理后,相比于H2O2组,RSV组β-半乳糖苷酶染色阳性细胞比例明显减低,细胞增殖能力增强,二者差异显著(P<0.05);相比于RSV组,H2O2组细胞周期多被阻滞在G1期,且细胞增殖相关蛋白(p-AKT和pERK)表达减少,而衰老相关蛋白p53、p21、p16、p-Rb表达明显增多;而RSV可拮抗H2O2对HUVECs的作用,使HUVECs凋亡细胞数显著减少,Survivin蛋白表达明显增多(P<0.05)。结论RSV可抑制氧化应激环境中HUVECs细胞的衰老及凋亡、促进细胞增殖,对HUVECs细胞具有保护作用,其机制与RSV改变相关蛋白表达有关。 展开更多
关键词 白藜芦醇 HUVECs细胞 衰老 增殖 细胞周期 凋亡
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二至丸抗大鼠肾细胞衰老的GCMS代谢组学研究 预览
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作者 徐佳 刘其南 +4 位作者 翟园园 冯丽 谢彤 姚卫峰 张丽 《南京中医药大学学报》 CAS CSCD 北大核心 2019年第4期458-464,共7页
目的研究D-半乳糖诱导的衰老大鼠肾细胞(NRK)内源性代谢物的变化,寻找与衰老有关的代谢生物标志物,探讨二至丸对衰老模型的调节作用及其抗衰老的可能机制.方法采用D-半乳糖复制NRK细胞衰老模型,借助气相色谱-质谱联用(GC-MS)技术,对各... 目的研究D-半乳糖诱导的衰老大鼠肾细胞(NRK)内源性代谢物的变化,寻找与衰老有关的代谢生物标志物,探讨二至丸对衰老模型的调节作用及其抗衰老的可能机制.方法采用D-半乳糖复制NRK细胞衰老模型,借助气相色谱-质谱联用(GC-MS)技术,对各组细胞样本进行测定,采用Kruskal-Wallis非参数检验及Dunn's多重比较进行单变量统计分析来筛选差异性代谢物.结果经PLS-DA模式识别分析,对照组、模型组和EZW组细胞样本能够得到很好的区分,共鉴定出31种潜在生物标志物,二至丸含药血清给药后衰老NRK细胞胞内的内源性代谢物水平发生不同程度的回调.结论二至丸可以使D-半乳糖诱导的衰老NRK细胞胞内的异常代谢有所恢复,其治疗作用可能与机体内31个代谢物及10条相关代谢通路的调节有关. 展开更多
关键词 衰老 二至丸 细胞代谢组学 GC-MS 大鼠肾细胞 含药血清
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作物STAYGREEN功能与调控的研究进展 预览
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作者 陈耀宇 王曙光 孙黛珍 《生物学杂志》 CAS CSCD 北大核心 2019年第3期83-87,共5页
STAYGREEN(SGR)/NON-YELLOWING(NYE)基因是一种编码叶片衰老相关蛋白的基因,该基因对叶绿素降解至关重要。许多植物的SGR基因突变体都会产生持绿表型,但不同SGR的功能有所差异。综述了SGR的功能、SGR与叶绿素代谢、SGR的调控3个方面的... STAYGREEN(SGR)/NON-YELLOWING(NYE)基因是一种编码叶片衰老相关蛋白的基因,该基因对叶绿素降解至关重要。许多植物的SGR基因突变体都会产生持绿表型,但不同SGR的功能有所差异。综述了SGR的功能、SGR与叶绿素代谢、SGR的调控3个方面的最新研究进展,并展望了SGR在中国主要粮食作物中的研究方向。 展开更多
关键词 SGR 持绿 叶绿素降解 衰老
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不同林龄红锥人工林细根垂直分布和衰老生理特征
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作者 吴敏 邓平 +2 位作者 赵英 钟道发 曾令鑫 《生态学杂志》 CAS CSCD 北大核心 2019年第9期2622-2629,共8页
以广西桂东和桂南地区3种林龄(7、14和25 a)红锥人工林细根为对象,在红锥生长旺季,采用根钻法,分表层(0~20 cm)和亚表层土壤(20~40 cm)获取细根,研究不同林龄红锥人工林细根在不同土层的形态、生物量以及衰老生理生化指标变化特征,以期... 以广西桂东和桂南地区3种林龄(7、14和25 a)红锥人工林细根为对象,在红锥生长旺季,采用根钻法,分表层(0~20 cm)和亚表层土壤(20~40 cm)获取细根,研究不同林龄红锥人工林细根在不同土层的形态、生物量以及衰老生理生化指标变化特征,以期深入认识红锥生长发育机制及林分生产力,为红锥人工林可持续经营提供依据。结果表明:在相同土层中,随林龄增加,红锥细根根长密度、根表面积密度、生物量、比表面积、细根活力、可溶性糖和可溶性蛋白含量均显著增加,而比根长、游离脯氨酸和丙二醛(MDA)含量显著减少;在相同林龄下,随土壤深度增加,红椎细根比根长、比表面积、细根活力、各渗透调节物质含量均显著增加,而根长密度、表面积密度、生物量、MDA含量显著减少;林龄和土层的交互作用对细根生物量、可溶性糖、可溶性蛋白和MDA含量有显著影响。细根根长密度、根表面积密度和生物量与各土壤因子间呈显著正相关;细根游离脯氨酸含量与各土壤因子间呈显著负相关;细根活力、可溶性糖和可溶性蛋白仅与土壤含水率、速效氮呈显著正相关。因此,7 a红锥细根易衰老,更新快,14 a林次之,25 a林细根更新慢;表层土(0~20cm)的红锥细根代谢旺盛,周转速率快,寿命较短。 展开更多
关键词 红锥 人工林 衰老 细根形态 生理生化
Late-entry commercial thinning effects on Pinus banksiana:growth,yield,and stand dynamics in Quebec,Canada 预览
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作者 Martin-Michel Gauthier Stephane Tremblay 《林业研究:英文版》 CAS CSCD 2019年第1期95-106,共12页
We studied late-entry commercial thinning effects on growth,yield,and regeneration in a 48-year-old jack pine(Pinus banksiana Lamb.)stand.Applied thinning intensities were 27,32,and 47%of merchantable basal area(BA)ex... We studied late-entry commercial thinning effects on growth,yield,and regeneration in a 48-year-old jack pine(Pinus banksiana Lamb.)stand.Applied thinning intensities were 27,32,and 47%of merchantable basal area(BA)excluding skidding trails.After 15 years,mean diameter at breast height of surviving trees in the 47%BA removal increased by 4.9 cm(25%)compared to the unthinned control.The 47%BA removal also increased gross merchantable volume(GMV)tree^-1 by 46%compared to the control.The 27%BA removal had twice as much GMV ha^-1 compared to the 47%BA removal after 15 years.Moreover,cumulative GMV ha^-1 was much higher in the 27%BA removal than in the unthinned control.The highest thinning intensity produced larger trees on average,while the lowest thinning intensity maximized volume production per hectare.Maintenance of acceptable growing stock throughout the 15-year period in the 27%BA removal could provide other ecosystem functions such as biodiversity enhancement or wildlife habitat by delaying senescence.Regeneration data showed that a shift in species composition occurred in the understory.After 15 years,the understory was dominated by black spruce(Picea mariana(Mill.)B.S.P.),white birch(Betula papyrifera Marsh.),and trembling aspen(Populus tremuloides Michx.).If regenerating jack pine is an objective after final overstory removal,additional efforts will be needed to re-establish this species. 展开更多
关键词 Jack PINE COMMERCIAL THINNING Stand YIELD SUCCESSION Delayed SENESCENCE Tree regeneration
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氯化钙处理对滑子菇采后贮藏品质的影响 预览 被引量:1
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作者 潘晓玉 刘晔 +3 位作者 宋静雅 阎文飞 李文香 程凡升 《中国果菜》 2019年第1期1-6,共6页
文章研究了氯化钙处理对滑子菇保鲜效果的影响。结果表明,氯化钙处理不仅降低了滑子菇的失重率、开伞率、褐变度和褐变的速率,而且还降低了膜的过氧化,延缓了滑子菇衰老,增强了滑子菇的抗氧化能力,有效保持滑子菇品质,减少了营养流失。... 文章研究了氯化钙处理对滑子菇保鲜效果的影响。结果表明,氯化钙处理不仅降低了滑子菇的失重率、开伞率、褐变度和褐变的速率,而且还降低了膜的过氧化,延缓了滑子菇衰老,增强了滑子菇的抗氧化能力,有效保持滑子菇品质,减少了营养流失。贮藏期间,滑子菇氯化钙处理组抗坏血酸含量始终高于对照组,有效保持滑子菇清除自由基能力。 展开更多
关键词 滑子菇 氯化钙 贮藏品质 衰老
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ECRG4: a new potential target in precision medicine
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作者 Xin Qin Ping Zhang 《医学前沿:英文版》 CAS CSCD 2019年第5期540-546,共7页
Given the rapid development in precision medicine, tremendous efforts have been devoted to discovering new biomarkers for disease diagnosis and treatment. Esophageal cancer-related gene-4 (ECRG4),which is initially kn... Given the rapid development in precision medicine, tremendous efforts have been devoted to discovering new biomarkers for disease diagnosis and treatment. Esophageal cancer-related gene-4 (ECRG4),which is initially known as a new candidate tumor suppressor gene, is emerging as a sentinel molecule for gauging tissue homeostasis. ECRG4 is unique in its cytokine-like functional pattern and epigenetically-regulated gene expression pattern. The gene can be released from the cell membrane upon activation and detected in liquid biopsy, thus offering considerable potential in precision medicine. This review provides an updated summary on the biology of ECRG4, with emphasis on its important roles in cancer diagnosis and therapy. The future perspectives of ECRG4 as a potential molecular marker in precision medicine are also discussed in detail. 展开更多
关键词 ECRG4 tumor SUPPRESSOR gene SENTINEL molecule PRECISION MEDICINE cell SENESCENCE EPITHELIUM homeostasis
Decreased numbers of circulating endothelial progenitor cells are associated with hyperglycemia in patients with traumatic brain injury 预览
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作者 Hui-Jie Wei Li Liu +7 位作者 Fang-Lian Chen Dong Wang Liang Wang Zeng-Guang Wang Rong-Cai Jiang Jing-Fei Dong Jie-Li Chen Jian-Ning Zhang 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第6期984-990,共7页
Hyperglycemia reduces the number of circulating endothelial progenitor cells,accelerates their senescence and impairs their function.However,the relationship between blood glucose levels and endothelial progenitor cel... Hyperglycemia reduces the number of circulating endothelial progenitor cells,accelerates their senescence and impairs their function.However,the relationship between blood glucose levels and endothelial progenitor cells in peripheral blood of patients with traumatic brain injury is unclear.In this study,101 traumatic brain injury patients admitted to the Department of Neurosurgery,Tianjin Medical University General Hospital or the Department of Neurosurgery,Tianjin Huanhu Hospital,China,were enrolled from April 2005 to March 2007.The number of circulating endothelial progenitor cells and blood glucose levels were measured at 1,4,7,14 and 21 days after traumatic brain injury by flow cytometry and automatic biochemical analysis,respectively.The number of circulating endothelial progenitor cells and blood sugar levels in 37 healthy control subjects were also examined.Compared with controls,the number of circulating endothelial progenitor cells in traumatic brain injury patients was decreased at 1 day after injury,and then increased at 4 days after injury,and reached a peak at 7 days after injury.Compared with controls,blood glucose levels in traumatic brain injury patients peaked at 1 day and then decreased until 7 days and then remained stable.At 1,4,and 7 days after injury,the number of circulating endothelial progenitor cells was negatively correlated with blood sugar levels(r=?0.147,P<0.05).Our results verify that hyperglycemia in patients with traumatic brain injury is associated with decreased numbers of circulating endothelial progenitor cells.This study was approved by the Ethical Committee of Tianjin Medical University General Hospital,China(approval No.200501)in January 2015. 展开更多
关键词 nerve REGENERATION endothelial progenitor cells VASCULAR repair VASCULAR remodeling angiogenesis NEOVASCULARIZATION blood glucose HYPERGLYCEMIA traumatic BRAIN injury MOBILIZATION suppression senescence alternative therapy BRAIN damage neural REGENERATION
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不同水分条件下小麦持绿相关性状与产量的关系 预览
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作者 陈耀宇 王曙光 +3 位作者 闫雪 梁增浩 史雨刚 孙黛珍 《山西农业科学》 2019年第6期991-997,共7页
雨养和灌溉2种水分条件下,观测小麦旱选10号/鲁麦14DH群体花后不同时期旗叶的功能绿叶面积(GLAD)和主茎绿叶数(GLNMS),并利用非线性回归分析计算小麦旗叶最大衰老速率(MRS)、衰老起始时间(Ts)、达到最大衰老速率的时间(TMRS)、衰老终止... 雨养和灌溉2种水分条件下,观测小麦旱选10号/鲁麦14DH群体花后不同时期旗叶的功能绿叶面积(GLAD)和主茎绿叶数(GLNMS),并利用非线性回归分析计算小麦旗叶最大衰老速率(MRS)、衰老起始时间(Ts)、达到最大衰老速率的时间(TMRS)、衰老终止时间(To)、最大衰老速率时GLAD的百分比(PGMS)、花后0d到旗叶保持3/4绿叶面积的天数(75%G)、花后0d到旗叶保持1/4绿叶面积的天数(25%G)和75%G与25%G之间的天数(50%G);成熟后测定单株产量(YPP)和千粒质量(TKW),然后进行性状间的相关分析。结果表明,2种水分条件下,DAA22(花后22d),DAA25(花后25d),DAA28(花后28d)和DAA31(花后31d)的旗叶GLAD均与DAA20(花后20d),DAA22和DAA30(花后30d)的GLNMS呈显著或极显著正相关,与MRS呈极显著负相关,与Ts,To,PGMS,TMRS,75%G,50%G和25%G呈显著或极显著正相关。GLNMS在开花后的不同时期与Ts,To,TMRS,75%G和25%G呈显著或极显著正相关,与50%G和PGMS的相关性不显著;DAA20,DAA25和DAA30的GLNMS与MRS呈显著或极显著负相关。干旱胁迫条件下,DAA16(花后16d),DAA19(花后19d),DAA22,DAA25,DAA28的GLAD以及灌溉条件下DAA16,DAA19,DAA22的GLAD均与千粒质量呈显著或极显著正相关,表明灌浆中期的GLAD对千粒质量影响较大。2种水分条件下,单株产量和千粒质量与50%G和Ts呈显著或极显著正相关,与MRS呈显著或极显著负相关,表明Ts与MRS是影响小麦产量的2个最主要的特征性状。育种实践中应该选育衰老起始晚、功能绿叶面积大、衰老缓慢即最大衰老速率较小的品种。 展开更多
关键词 小麦 衰老 持绿性 产量 相关性
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