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MicroRNAs in blood and cerebrospinal fluid as diagnostic biomarkers of multiple sclerosis and to monitor disease progression 预览
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作者 Bridget Martinez Philip V.Peplow 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期606-619,共14页
Multiple sclerosis is a chronic autoimmune disease of the central nervous system.It is the main cause of non-traumatic neurological disability in young adults.Multiple sclerosis mostly affects people aged 20–50 years... Multiple sclerosis is a chronic autoimmune disease of the central nervous system.It is the main cause of non-traumatic neurological disability in young adults.Multiple sclerosis mostly affects people aged 20–50 years;however,it can occur in young children and much older adults.Factors identified in the distribution of MS include age,gender,genetics,environment,and ethnic background.Multiple sclerosis is usually associated with progressive degrees of disability.The disease involves demyelination of axons of the central nervous system and causes brain and spinal cord neuronal loss and atrophy.Diagnosing multiple sclerosis is based on a patient’s medical history including symptoms,physical examination,and various tests such as magnetic resonance imaging,cerebrospinal fluid and blood tests,and electrophysiology.The disease course of multiple sclerosis is not well correlated with the biomarkers presently used in clinical practice.Blood-derived biomarkers that can detect and distinguish the different phenotypes in multiple sclerosis may be advantageous in personalized treatment with disease-modifying drugs and to predict response to treatment.The studies reviewed have shown that the expression levels of a large number of miRNAs in peripheral blood,serum,exosomes isolated from serum,and cerebrospinal fluid are altered in multiple sclerosis and can distinguish the disease phenotypes from each other.Further studies are warranted to independently validate these findings so that individual or pairs of miRNAs in serum or cerebrospinal fluid can be used as potential diagnostic markers for adult and pediatric multiple sclerosis and for monitoring disease progression and response to therapy. 展开更多
关键词 clinically isolated syndrome CSF disease PROGRESSION EXOSOMES humans microRNA multiple SCLEROSIS PERIPHERAL blood PHENOTYPES serum
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Spatiotemporal expression of leukemia inhibitory factor receptor protein during neural tube development in embryos with neural tube defects 预览
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作者 Dong An Xiao-Wei Wei +3 位作者 He-Nan Zhang Dan Liu Wei Ma Zheng-Wei Yuan 《中国神经再生研究:英文版》 SCIE CAS CSCD 2020年第4期705-711,共7页
Leukemia inhibitory factor receptor(LIFR),as a neuroregulatory cytokine receptor,generally shows a neuroprotective effect in central nervous system injuries.In this study,to understand the effect of LIFR on pathogenes... Leukemia inhibitory factor receptor(LIFR),as a neuroregulatory cytokine receptor,generally shows a neuroprotective effect in central nervous system injuries.In this study,to understand the effect of LIFR on pathogenesis of neural tube defects,we explored spatiotemporal expression of LIFR at different stages of fetal development in normal and neural tube defect embryos.Spina bifida aperta was induced with all-trans retinoic acid on embryonic day 10 in rats,and the spatiotemporal expression of LIFR was investigated in spina bifida aperta rats and healthy rats from embryonic day 11 to 17.Real time-polymerase chain reaction and western blot assay were used to examine mRNA and protein expression of LIFR in healthy control and neural tube defect embryos.Results of the animal experiment demonstrated that expression of LIFR protein and mRNA in the spinal cords of normal rat embryos increased with embryonic development.LIFR was significantly downregulated in the spinal cords of spina bifida aperta rats compared with healthy rats from embryonic days 11 to 17.Immunohistochemical staining showed that the expression of LIFR in placenta and spinal cord in spina bifida aperta rat embryos was decreased compared with that in control embryos at embryonic day 15.Results from human embryo specimens showed that LIFR mRNA expression was significantly down-regulated in spinal cords of human fetuses with neural tube defects compared with normal controls at a gestational age of 24 to 33 weeks.The results were consistent with the down-regulation of LIFR in the animal experiments.Our study revealed spatiotemporal changes in expression of LIFR during embryonic neurulation.Thus,LIFR might play a specific role in neural tube development.All animal and human experimental procedures were approved by the Medical Ethics Committee of Shengjing Hospital of China Medical University,China(approval No.2016PS106K)on February 25,2016. 展开更多
关键词 amniotic fluid DEVELOPMENT EMBRYOGENESIS LEUKEMIA INHIBITORY factor receptor nerve regeneration neural tube defect PLACENTA SPATIOTEMPORAL expression spina bifida aperta spinal cord serum
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Effects of Gender, Age, and Clot Formation on the Measurements of Tryptophan Metabolites in Blood 预览
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作者 Junichi Masuda Masato Karayama +3 位作者 Takafumi Suda Masato Maekawa Fumiko Shimizu Akikazu Takada 《食品与营养科学(英文)》 2019年第7期761-775,共15页
Background: Tryptophan metabolism plays important roles in health and diseases. Although simultaneous measurements of tryptophan metabolites are successfully measured, influences of age, gender, and clot formation on ... Background: Tryptophan metabolism plays important roles in health and diseases. Although simultaneous measurements of tryptophan metabolites are successfully measured, influences of age, gender, and clot formation on the measurements have not been reported. Methods: We took blood from young and old Japanese men and women and compared plasma levels of tryptophan metabolites. We also took plasma and serum from the blood of middle-aged men (n = 10). Metabolites analysis was performed by a liquid chromatograph tandem mass spectrometer, the LCMS-8060 quadrupole mass spectrometer combined with Nexera X2 liquid chromatograph system (Shimadzu Corporation, Kyoto, Japan). Body mass index (BMI) and TRP metabolites have been measured in healthy young men (n = 48), young women (n = 47), old men (n = 44), and old women (n = 39). TRP metabolites were measured by using the ultrahigh speed liquid chromatography-mass spectroscopy (Shimadzu Corporation). Results: Tryptophan and its metabolites such as serotonin, 5-hydroxyindole acetic acid, indole-3-acetic acid, kynurenine, anthranilic acid, were higher in young women and old men than young men and old women. Plasma levels of 3-hydroxykynurenine and xanthurenic acid were lower in young women and old men. Comparison of plasma and serum indicates that most of metabolites were higher in serum than plasma except for 3-hydroxy-kynurenine and quinolinic acid. Conclusion: Metabolites of the upper stream of degradation of tryptophan were higher in young women and old men than young men and old women, which suggests that the degradation of tryptophan was accelerated in young men and old women than young women and old men. Serum preparation may activate tryptophan degradation resulting in higher levels of metabolites in serum than in plasma. 展开更多
关键词 Plasma Serum Platelet TRYPTOPHAN SEROTONIN 5-Hydroxyindole Acetic ACID KYNURENINE 3-Hydroxykynurenine Kynurenic ACID Anthranilic ACID Xanthurenic ACID Indole-3-Acetic ACID
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Seroprevalence of Cryptosporidium and risks of cryptosporidiosis in residents of Sothern Egypt: A cross-sectional study
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作者 Ismail Elshahawy Fatma AbouElenien 《亚太热带医药杂志:英文版》 SCIE CAS 2019年第5期232-238,共7页
Objective: To identify the serological epidemiology of Cryptosporidium infections and to follow up on the changes in the infection profile in Southern Egypt in order to establish a suitable scheme for control and prev... Objective: To identify the serological epidemiology of Cryptosporidium infections and to follow up on the changes in the infection profile in Southern Egypt in order to establish a suitable scheme for control and prevention of cryptosporidiosis.Methods: A total of 1 912(960 from human and 952 from animals) stool specimens and sera were screened for Cryptosporidium species using modified Ziehl Neelsen technique and a newly-developed enzyme-linked immunosorbent assay(ELISA).Environmental risk factors and socioeconomic data were surveyed by questionnaire between September 2016 and December 2017.Results: Totally, 20.83% of the human subjects were positive for Cryptosporidium infection tested by ELISA.The seropositivity was positively correlated with age.The prevalence of Cryptosporidium infections in females was significantly higher than in males(P<0.05).The sensitivity and specificity of ELISA for Cryptosporidium were 99.06% and 88.88%, respectively.Furthermore, a high prevalence of Cryptosporidium in domestic animals(42.20%).Conclusions: The study observed that Cryptosporidium infections are common in the study area, with water sanitation, socioeconomic level;eating habits and hygienic status are considered the main risk factors for cryptosporidiosis.Therefore, environmental sanitation and health education will be useful in reducing the prevalence of infection. 展开更多
关键词 CRYPTOSPORIDIUM EPIDEMIOLOGY ELISA test Risk factors Serum EGYPT
Contrast Induced Nephropathy after Radial or Femoral Access for Invasive Management of Acute Coronary Syndrome 预览
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作者 Neveen I. Samy Walaafareed   +2 位作者 Ahmed Abdelbaky S. Ahmed Mohamed Osama 《心血管病(英文)》 2019年第8期572-583,共12页
Background: Percutaneous coronary intervention is now the best way of management of acute coronary syndrome (ACS). Contrast induced nephropathy is a serious complication and greatly dependent on several factors. It is... Background: Percutaneous coronary intervention is now the best way of management of acute coronary syndrome (ACS). Contrast induced nephropathy is a serious complication and greatly dependent on several factors. It is still unclear whether the vascular access migrates CIN risk. Objective: To study the impact of Radial Access (RA) compared with Femoral Access (FA) on developing contrast-induced nephropathy (CIN) in patients undergoing invasive management of acute coronary syndrome (ACS). Methods: Sixty patients eligible for invasive management of ACS at cardiology department (Menoufia University hospital and National Heart Institute) were randomized into two groups. Group I: included 30 patients with femoral approach and Group II: included 30 patients with radial approach. The occurrence of CIN estimated by KDIGO definition (absolute increase in serum creatinine (SCr) by ≥0.5 mg/dl within 48 hours;or increase in SCr to ≥25% of baseline) was estimated in both groups. Results: Only 9 patients (15%) developed CIN, 5 patients (55.6%) of them underwent PCI through FA without statistically significant difference between the two approaches.Conclusion: CIN is considered a potential complication of percutaneous coronary intervention (PCI). Our study did not show the preference of using an approach over the other. 展开更多
关键词 CONTRAST Induced NEPHROPATHY Serum CREATININE PERCUTANEOUS Coronary Intervention
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Clinical Significance of Serum Galectin-1 and Its Tissue Immunohistochemical Expression in Serous Ovarian Carcinoma Patients 预览
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作者 Mai Mohamed Abdelwahab Huda Fathy Ebian +5 位作者 Taiseer Refat Ibrahim Mohammad Samir Badr Mohamed El-Bakry Lashin Mahmoud Abdou Yassin Adel Mohamed Ismail Ahmed Ali Obaya 《妇产科期刊(英文)》 2019年第7期937-953,共17页
Objectives: Serous ovarian carcinoma (SOC) is the commonest ovarian carcinoma type with poor prognosis due to early metastasis and first presentation with advanced stage. In this work, we investigated serum level of G... Objectives: Serous ovarian carcinoma (SOC) is the commonest ovarian carcinoma type with poor prognosis due to early metastasis and first presentation with advanced stage. In this work, we investigated serum level of Galactin-1 (Gal-1) and its tissue immunohistochemical expression in SOC patients at different stages trying to find out its significance as a diagnostic and prognostic marker. Patients and methods: The study included 95 females I-Control group: Twenty five healthy females;II-Patients group: Seventy females diagnosed as SOC at different stages;Stage I: 8 cases, Stage II: 12 cases, Stage III: 32 cases and Stage VI:18 cases. Serum Galectin-1 and CA-125 were measured by ELIZA and tissue Galectin-1 was assessed by immunohistochemistry. All patients were followed for up to 3 years after surgery. Results: Serum Gal-1 and CA-125 levels were significantly higehr in SOC patients compared to controls (p 0.001). We found a direct positive statistically significant correlation between serum Gal-1 and CA125 levels (p 0.001). Serum Gal-1 at cut off value > 135 ng/ml was superior to CA-125 a cut off value > 49 u/ml with sensitivity, specificity of 100%, vs 88.57, 96% for CA-125. Serum Gal-1 was significantly associated with tumor stage (p 0.001). Immunohistochemistry showed that patients with strong Gal-1 expression had higher serum level (p = 0.002). Stromal and tumor Gal-1 expression were significantly correlated with tumor grade (p 0.001) and stage (p = 0.001). Serum Gal-1, CA-125 and IHC Gal-1 expression were associated with poor survival (p 0.001, p = 0.009 and p = 0.002) respectively. Conclusion: Serum Gal-1 and its tissue IHC expression are useful diagnostic and prognostic markers for SOC patients. 展开更多
关键词 SEROUS OVARIAN Carcinoma SERUM IMMUNOHISTOCHEMISTRY GALECTIN-1 Overall Survival
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连云港地区健康成人血清尿酸浓度的分布及其参考区间的建立
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作者 刘倩 杨伏猛 +3 位作者 何传梅 王舜 陈伟民 刘晓东 《中国临床实用医学》 2019年第2期17-20,共4页
目的探讨连云港地区20~79岁健康成人血清尿酸的分布情况,并初步建立连云港地区健康成人血清尿酸的参考区间。方法采用完全随机化方法选取2017年7月至2018年6月连云港市第二人民医院体检中心的健康志愿者29 304例,男16 066例,女13 238例... 目的探讨连云港地区20~79岁健康成人血清尿酸的分布情况,并初步建立连云港地区健康成人血清尿酸的参考区间。方法采用完全随机化方法选取2017年7月至2018年6月连云港市第二人民医院体检中心的健康志愿者29 304例,男16 066例,女13 238例,年龄20~79岁。将志愿者按照性别分为男性组和女性组,再按照年龄将两组分别分为男(20~29岁)组、男(30~39岁)组、男(40~49岁)组、男(50~59岁)组、男(60~69岁)组、男(70~79岁)组和女(20~29岁)组、女(30~39岁)组、女(40~49岁)组、女(50~59岁)组、女(60~69岁)组、女(70~79岁)组。采用AU5821全自动生化分析仪及其配套检测试剂测定血清尿酸浓度,并依据CLSI C28-A3和WS/T 402-2012《临床实验室检验项目参考区间的制定》文件建立血清尿酸的参考区间。结果连云港地区健康成人血清中尿酸的浓度分布在性别方面比较,差异有统计学意义(P<0.05),但在年龄方面比较,差异无统计学意义(P>0.05),故依据性别建立血清尿酸参考区间,男(20~79岁)为233~539 μmol/L;女(20~79岁)为170~411 μmol/L。结论血清尿酸参考区间的建立,能够为临床相关疾病的辅助诊断及临床决策的制定提供重要的指导价值。 展开更多
关键词 血清 尿酸 性别 参考区间
基于L-NAME诱导的高血压小鼠血清代谢组学研究 预览
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作者 杜梦繁 张光远 +2 位作者 王志玮 周婷婷 马鑫 《中国药理学通报》 CAS CSCD 北大核心 2019年第12期1775-1776,共2页
高血压是心脑血管的主要危险因素,是导致我国居民死亡的主要原因之一[1-2]。高血压的发生和发展受遗传因素和环境因素的共同影响[3],而在近年来的研究中,造成高血压的主要因素60%与代谢异常有关,约80%的高血压患者存在各种形式的代谢紊... 高血压是心脑血管的主要危险因素,是导致我国居民死亡的主要原因之一[1-2]。高血压的发生和发展受遗传因素和环境因素的共同影响[3],而在近年来的研究中,造成高血压的主要因素60%与代谢异常有关,约80%的高血压患者存在各种形式的代谢紊乱[4]。 展开更多
关键词 高血压 代谢组学 UPLC-QTOF/MS L-NAME 血清 差异分子
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基于GIS的广西原发性肝癌患者血清微囊藻毒素-LR的空间分布
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作者 贾雪姣 农清清 +8 位作者 黄海 赵慧柳 唐咸艳 麦浩 李亚林 黄秋月 关斌 费梦雪 马智星 《环境与健康杂志》 CAS 北大核心 2019年第4期335-337,376共4页
目的探讨广西原发性肝癌患者血清中微囊藻毒素-LR(microcystin-LR,MC-LR)的空间分布规律。方法采集2015—2017年广西14个地级市原发性肝癌患者的静脉血,并采集当地的生活饮用水,采用酶联免疫吸附法测定血清和饮用水中的MC-LR浓度。采用... 目的探讨广西原发性肝癌患者血清中微囊藻毒素-LR(microcystin-LR,MC-LR)的空间分布规律。方法采集2015—2017年广西14个地级市原发性肝癌患者的静脉血,并采集当地的生活饮用水,采用酶联免疫吸附法测定血清和饮用水中的MC-LR浓度。采用地理信息系统(geographic information system,GIS)空间插值分析技术和全局空间自相关评估广西肝癌患者血清中MC-LR浓度的空间分布特征及空间聚集性。采用GIS叠加分析观察血清中MC-LR与饮用水中MC-LR的空间分布吻合程度,并采用Pearson相关分析两者的相关程度。结果 GIS绘图显示广西肝癌患者血清中MC-LR浓度呈现'南高北低'的分布规律:高值地区主要包括崇左市、南宁市、防城港市和钦州市,低值地区主要包括桂林市等。全局自相关分析结果显示,广西肝癌患者血清中MC-LR浓度存在空间聚集性。GIS叠加分析显示,肝癌患者血清中MC-LR浓度的空间分布与饮用水中MC-LR浓度的空间分布吻合度较高。肝癌患者血清中MC-LR浓度与饮用水中MC-LR浓度呈统计学正相关(P<0.05)。结论广西地区原发性肝癌患者血清MC-LR浓度存在空间异质性,可能与当地饮用水中MC-LR的水平有关。 展开更多
关键词 肝癌 血清 饮用水 微囊藻毒素-LR 地理信息系统
高热惊厥患儿血清和脑脊液中MMP9及IL-18水平的研究 预览
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作者 王均林 王淑丽 +1 位作者 李玉芬 徐丽云 《山东医学高等专科学校学报》 2019年第2期105-107,共3页
目的探讨评估高热惊厥患儿脑损伤的方法。方法选取96例高热惊厥患儿,将其分为复杂性高热惊厥组(46例)和单纯性高热惊厥组(50例);另选同期住院的40例患儿为对照组。使用ELISA法测定各组血清和脑脊液中MMP9和IL-18水平。结果复杂性高热惊... 目的探讨评估高热惊厥患儿脑损伤的方法。方法选取96例高热惊厥患儿,将其分为复杂性高热惊厥组(46例)和单纯性高热惊厥组(50例);另选同期住院的40例患儿为对照组。使用ELISA法测定各组血清和脑脊液中MMP9和IL-18水平。结果复杂性高热惊厥组血清和脑脊液中MMP9和IL-18水平最高,单纯性高热惊厥组次之,对照组最低(q=14.59~77.99,P<0.05)。结论MMP9和IL-18是评估高热惊厥患儿脑损伤程度及预后的指标。 展开更多
关键词 MMP9 IL-18 高热惊厥 血清 脑脊液
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间歇蓝光照射联合静脉滴注白蛋白治疗新生儿病理性黄疸患儿的临床疗效 预览
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作者 陈海云 崔华英 陈博文 《中华妇幼临床医学杂志(电子版)》 CAS 2019年第5期579-584,共6页
目的 探讨不同蓝光照射方式联合静脉滴注白蛋白治疗新生儿病理性黄疸的临床疗效及其安全性。方法 选取2015年2月至2017年10月,东营市东营区人民医院收治的82例新生儿病理性黄疸患儿为研究对象。采用掷硬币随机方法进行分组,将其分为实验... 目的 探讨不同蓝光照射方式联合静脉滴注白蛋白治疗新生儿病理性黄疸的临床疗效及其安全性。方法 选取2015年2月至2017年10月,东营市东营区人民医院收治的82例新生儿病理性黄疸患儿为研究对象。采用掷硬币随机方法进行分组,将其分为实验组(n=41,接受间歇蓝光照射+静脉滴注白蛋白)和对照组(n=41,接受连续蓝光照射+静脉滴注白蛋白)。采用成组t检验,对2组患儿的新生儿出生体重、胎龄,以及血清总胆红素(TBIL)、直接胆红素(DBIL)、间接胆红素(IBIL)、超敏C反应蛋白(HS-CRP)、总胆汁酸(TBA)、游离脂肪酸(FFA)、碱性磷酸酶(AKP)及γ-谷氨酰转肽酶(γ-GT)水平等进行统计学比较。采用χ2检验,对2组患儿的性别构成比、治疗总有效率及不良反应率进行统计学分析。本研究遵循的程序符合东营市东营区人民医院伦理委员会所制定的伦理学标准,得到该委员会批准(批准文号:20150103),所有受试儿监护人均签署临床研究知情同意书。结果 ①2组患儿的性别构成比、新生儿出生体重和胎龄分别比较,差异均无统计学意义(P>0.05)。②实验组患儿治疗后第7天血清TBIL、DBIL与IBIL水平分别为(153.9±9.6)μmol/L、(8.2±2.4)μmol/L和(139.6±8.9)μmol/L,均显著低于治疗前的(208.2±11.2)μmol/L、(20.9±2.8)μmol/L和(191.3±8.1)μmol/L,组内分别比较,差异均有统计学意义(t=23.549、23.055、27.429,P<0.001)。对照组患儿治疗后第7天血清TBIL、DBIL与IBIL水平分别为(157.4±7.9)μmol/L、(8.9±1.5)μmol/L、(142.0±7.2)μmol/L,亦显著低于治疗前的(210.8±10.9)μmol/L、(21.3±3.1)μmol/L、(192.9±8.9)μmol/L,并且差异均有统计学意义(t=25.400、22.154、28.470,P<0.001)。此外,2组患儿治疗前与治疗后第7天的血清TBIL、DBIL与IBIL水平分别组间比较,差异均无统计学意义(P>0.05)。③实验组患儿治疗后第7天的HS-CRP、TBA、FFA、AKP和γ-GT水平分别为(1.3±0.3)mg/L、(12.3±3.4)μm 展开更多
关键词 黄疸 新生儿 光疗法 血清 胆红素 C反应蛋白质 婴儿 新生
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不同病理类型肺癌早期诊断中血清标志物联合检测的应用分析 预览
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作者 阮福明 《黑龙江医学》 2019年第1期74-76,共3页
目的探讨血清多项指标联合检测在不同病理类型肺癌诊断中的应用价值。方法比较检测指标单项检查与联合检查在不同类型肺癌中的敏感性和特异性。结果本研究采用的检测指标为癌胚抗原(CEA)、神经原特异烯醇化酶(NSE)、细胞角蛋白19片断(CY... 目的探讨血清多项指标联合检测在不同病理类型肺癌诊断中的应用价值。方法比较检测指标单项检查与联合检查在不同类型肺癌中的敏感性和特异性。结果本研究采用的检测指标为癌胚抗原(CEA)、神经原特异烯醇化酶(NSE)、细胞角蛋白19片断(CYFRA21-1)、胃泌素释放肽前体(ProGRP)、鳞状细胞癌相关抗原(SCC)等5种。在小细胞肺癌的诊断中,CEA+NSE+ProGRP方案的敏感性和特异性分别为90.0%和92.1%,明显高于大细胞肺癌的的敏感性和特异性;在鳞癌的诊断中,CEA+CYFRA21-1+SCC敏感性和特异性分别为91.1%和93.6%,明显高于小细胞肺癌和腺癌的敏感性和特异性;CEA+NSE+CYFRA21-1对各种类型肺癌的诊断具有良好的敏感性和特异性,可用于肺癌筛查。结论联合检测过多肿瘤标志物增加了患者的经济负担,对肺癌的早期诊断的敏感性和特异性无明显影响,不推荐使用。 展开更多
关键词 血清 肿瘤标志物 联合检测 病理类型 小细胞肺癌
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血清微小RNA-21水平与糖尿病肾病患者氧化应激的相关性研究
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作者 佀思聪 杨伟 +3 位作者 罗鸿宇 王洁妤 刘佳 赵欢 《现代生物医学进展》 CAS 2019年第9期1786-1789,共4页
目的:探讨血清微小RNA-21(miR-21)水平与糖尿病肾病(DN)患者氧化应激的相关性。方法:选择2015年1月到2017年5月我院就诊的169例DN患者作为研究对象,其中DN分期早期组95例,中晚期组74例。另选同期在我院接受健康体检者90例作为对照组,对... 目的:探讨血清微小RNA-21(miR-21)水平与糖尿病肾病(DN)患者氧化应激的相关性。方法:选择2015年1月到2017年5月我院就诊的169例DN患者作为研究对象,其中DN分期早期组95例,中晚期组74例。另选同期在我院接受健康体检者90例作为对照组,对比各组血清mi R-21水平和氧化应激指标,分析患者血清mi R-21水平与氧化应激指标的相关性。结果:各组血清mi R-21水平相比差异有统计学意义(P<0.05),中晚期DN组及早期DN组血清mi R-21水平均明显低于对照组,且中晚期DN组又明显低于早期DN组,差异均有统计学意义(P<0.05)。各组氧化应激指标相比差异有统计学意义(P<0.05),中晚期DN组及早期DN组丙二醛(MDA)、超氧化物歧化酶(SOD)、晚期氧化蛋白产物(AOPP)及NADPH氧化酶4(NOX4)均明显高于对照组,且中晚期DN组又明显高于早期DN组;而血红素氧合酶1(HO-1)明显低于对照组,且中晚期DN组又明显低于早期DN组,差异均有统计学意义(P<0.05)。依据Spearman法分析相关性发现,血清miR-21水平与MDA、SOD、AOPP及NOX4均呈负相关(P<0.05),与HO-1呈正相关(P<0.05)。结论:血清mi R-21水平与DN患者氧化应激指标均具有明显的相关性,临床上可尝试将miR-21纳入到DN患者病情监测的指标体系中,从而有助于疾病的诊治及患者的预后。 展开更多
关键词 糖尿病肾病 血清 MIR-21 氧化应激 相关性
Effect of Pelleted Total Mixed Ration on Production Performance and Serum Biochemical Index of Growing Hainan Black Goats 预览
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作者 Liu Quanwei Wang Feng +4 位作者 Wei Limin Huang Lili Sun Ruiping Xing Manping Zheng Xinli 《动物与饲料科学:英文版》 CAS 2019年第1期19-22,31共5页
[Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with ... [Objective] The paper was to investigate effects of pelleted total mixed ration on production performance and serum biochemical index of growing Hainan black goats.[Method] Twenty-four growing Hainan black goats with the same genetic backgrounds and similar initial weight of(10.05±0.049) kg were randomly assigned into two treatments, and supplied with Juncao + concentrate(control group), pelleted total mixed ration(experimental group), respectively. The trial lasted 50 d.[Result] The final weight, daily gain and feed intake of experimental group were significantly higher than those of control group(P<0.05), but the feed/gain ratio of experimental group was markedly lower than that of control group(P<0.05). Compared with the control group, the gross profit of experimental group significantly increased( P <0.05).[Conclusion] Pelleted total mixed ration led to better production performance of growing Hainan black goat. 展开更多
关键词 GROWING HAINAN black GOAT Pelleted total mixed RATION Production performance SERUM BIOCHEMICAL parameters
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Effect of Pelleted Total Mixed Ration of Different Particle Sizes on Production Performance and Serum Biochemical Index of Fattening Hainan Black Goats 预览
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作者 Liu Quanwei Wei Limin +4 位作者 Wang Feng Tan Shuyi Sun Ruiping Huang Lili Zheng Xinli 《动物与饲料科学:英文版》 CAS 2019年第1期23-27,共5页
[Objective] The paper to investigate the effects of pelleted total mixed ration of different particle size on production performance and serum biochemical index of fattening Hainan black goats.[Method] Thirty-six fatt... [Objective] The paper to investigate the effects of pelleted total mixed ration of different particle size on production performance and serum biochemical index of fattening Hainan black goats.[Method] Thirty-six fattening Hainan black goats with the same genetic backgrounds and similar initial weight of(19.02±0.22) kg were randomly divided into three treatments. Goats were fed with Juncao+concentrate(control group), pel-leted total mixed ration I(particle size 6 mm, experimental group I) and II(particle size 5 mm, experimental group II), respectively. The trial lasted 45 d.[Result](1) The final weight and daily gain of experimental group II were significantly higher than those of control group( P<0.05), and the daily gain of experimental group II was also higher than that of experimental group I(P<0.05);the feed intake of experimental group II was markedly higher than that of control group(P<0.05), but was significantly lower than that of experimental group I(P<0.05). The feed/gain ratio of experimental group II was significantly lower than those of control group and experimental group I(P<0.05).(2) The feed gain cost of experimental group II was markedly lower than that of control group(P<0.05), but the gross profit was significantly higher than those of control group and experimental group I(P<0.05).(3) Compared with the control group, the serum total protein content of experimental group II increased markedly( P<0.05) while the urea nitrogen level decreased(P>0.05).[Conclusion] The pelleted total mixed ration with the particle size of 5 mm obtained better fattening ef-fect, and obviously improved the serum total protein level. 展开更多
关键词 FATTENING HAINAN black GOAT Pelleted total mixed RATION Particle size Production performance SERUM BIOCHEMICAL parameters
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器官移植术后高尿酸血症的研究进展 预览
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作者 李韵 林俊 《实用器官移植电子杂志》 2019年第3期221-225,共5页
近年来,随着经济的发展、人们生活方式和饮食结构的改变及免疫抑制剂等药物的广泛使用,高尿酸血症(hyperuricemia,HUA)的发病率呈逐年增高趋势,是器官移植术后的常见并发症。HUA 是由于嘌呤代谢障碍导致尿酸合成增加和(或)排泄减少的一... 近年来,随着经济的发展、人们生活方式和饮食结构的改变及免疫抑制剂等药物的广泛使用,高尿酸血症(hyperuricemia,HUA)的发病率呈逐年增高趋势,是器官移植术后的常见并发症。HUA 是由于嘌呤代谢障碍导致尿酸合成增加和(或)排泄减少的一种代谢性疾病,国际上将男性血尿酸(serum uric acid,SUA)水平高于420 μmol/L,女性高于360 μmol/L 定义为HUA 的诊断标准。HUA 的患病率受多种因素影响。 展开更多
关键词 高尿酸血症 器官移植术 嘌呤代谢障碍 免疫抑制剂 常见并发症 代谢性疾病 serum HUA
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血清降钙素原对骨折内固定术后深部感染早期诊断的应用研究
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作者 蒙宇志 肖忠武 +2 位作者 聂杰辉 韦世强 李小炎 《中国伤残医学》 2019年第11期16-18,共3页
目的:研究血清降钙素原用于骨折内固定术后深部感染早期诊断的应用。方法:此文研究资料是2016年11月-2018年12月广东省德庆县人民医院骨外科病房行骨折内固定术治疗术后疑似手术部位感染的110例患者,比对手术部位感染组、非手术部位感... 目的:研究血清降钙素原用于骨折内固定术后深部感染早期诊断的应用。方法:此文研究资料是2016年11月-2018年12月广东省德庆县人民医院骨外科病房行骨折内固定术治疗术后疑似手术部位感染的110例患者,比对手术部位感染组、非手术部位感染组血清降钙素原检测值、C反应蛋白检测值,且评估深部感染组、浅表感染组血清降钙素原检测值。结果:手术部位感染组血清降钙素原检测值明显高于非手术部位感染组相关数值,深部感染组血清降钙素原检测值明显高于浅表感染组相关数值,P<0.05,体现组间指标显著统计学意义,血清降钙素原诊断手术部位感染的灵敏度是33.33%,特异度是99.07%。结论:在骨折内固定术后深部感染早期诊断采取血清降钙素原指标检测的特异度高,但灵敏度低,不适宜用作骨折内固定术患者术后深部感染早期诊断的指标。 展开更多
关键词 血清 降钙素原 骨折内固定术 术后深部感染 早期诊断
不同标本类型C反应蛋白检测结果的一致性分析 预览
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作者 王鹏 江海洋 +3 位作者 赵超 徐广峰 陈诚 谢晖 《东南国防医药》 2019年第3期254-257,共4页
目的探讨PENTRAMSCRP分析仪检测全血标本与TBA2000FR全自动生化分析仪检测血浆、血清标本C反应蛋白(CRP)结果的可比性及相关性。方法随机留取2017年5-7月东部战区总医院(原八二医院)93例门诊及住院患者EDTA-K2抗凝血与非抗凝血标本(57... 目的探讨PENTRAMSCRP分析仪检测全血标本与TBA2000FR全自动生化分析仪检测血浆、血清标本C反应蛋白(CRP)结果的可比性及相关性。方法随机留取2017年5-7月东部战区总医院(原八二医院)93例门诊及住院患者EDTA-K2抗凝血与非抗凝血标本(57例抗凝标本,36例血清标本),分别采用PENTRAMSCRP分析仪检测全血标本CRP浓度,TBA2000FR全自动生化分析仪检测血浆与血清CRP浓度。根据全血CRP浓度,将受试者分为低值组(<5μg/mL)、中值组(5~20μg/mL)和高值组(>20μg/mL),分别对全血CRP浓度与血浆、血清CRP浓度进行比对分析。结果在低值组、中值组及高值组中,全血CRP与血浆CRP之间(r值分别为0.941、0.974、0.964),全血CRP与血清CRP之间(r值分别为0.995、0.966、0.996)均呈良好的相关性,差异均有统计学意义(P<0.05),其中全血CRP与血清CRP之间差异在临床可接受范围内。结论血浆标本不适于直接在TBA2000FR全自动生化分析仪检测,全血标本与血清标本分别在2个检测系统的检验结果具有一致性。 展开更多
关键词 C反应蛋白 全血 血浆 血清 对比分析
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青海省人群维生素D水平与甲状腺过氧化物酶水平的关系
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作者 米娜 崇显瑾 +3 位作者 李正 杨历新 李国彤 姜志皛 《中华地方病学杂志》 CAS CSCD 北大核心 2019年第3期185-188,共4页
目的探讨青海省人群维生素D水平与甲状腺过氧化物酶(TPO)水平的关系。方法2015、2016年,采取分层整群抽样方法抽取青海省9个地区人群1 873例,其中男性935人、女性938人,年龄为(52.3 ± 10.9)岁,汉族1 040人、藏族833人。采集人群空... 目的探讨青海省人群维生素D水平与甲状腺过氧化物酶(TPO)水平的关系。方法2015、2016年,采取分层整群抽样方法抽取青海省9个地区人群1 873例,其中男性935人、女性938人,年龄为(52.3 ± 10.9)岁,汉族1 040人、藏族833人。采集人群空腹静脉血,采用全自动化学发光免疫分析系统检测维生素D和TPO水平,分析不同性别、民族、海拔高度人群维生素D和TPO水平。结果人群维生素D水平为(16.3 ± 7.5)μg/L,维生素D异常率为89.2%(1 670/1 873);TPO水平[中位数(四分位数)]为23.8(16.0 ~ 35.0)U/ml,TPO阳性率为26.8%(502/1 873)。男、女性维生素D水平分别为(16.9 ± 7.6)、(15.6 ± 7.6)μg/L,二者比较差异有统计学意义(t = 3.684,P < 0.01);TPO水平分别为23.8(16.4 ~ 31.0)、23.8(15.9 ~ 37.6)U/ml,二者比较差异无统计学意义(Z =- 1.084,P > 0.05)。汉族、藏族人群维生素D水平分别为(16.2 ± 7.8)、(16.3 ± 7.3)μg/L,二者比较差异无统计学意义(t =- 0.110,P > 0.05);TPO水平分别为23.5(15.7 ~ 34.8)、24.0(16.5 ~ 35.1)U/ml,二者比较差异无统计学意义(Z =- 0.078,P > 0.05)。中海拔(1 500 ~ 3 000 m)、高海拔(> 3 000 m)人群维生素D水平分别为(16.6 ± 7.7)、(15.8 ± 7.5)μg/L,二者比较差异无统计学意义(t = 2.126,P > 0.05);TPO水平分别为23.4(16.0 ~ 33.0)、24.0(16.0 ~ 36.8)U/ml,二者比较差异无统计学意义(Z =- 1.296,P > 0.05)。维生素D水平与TPO水平、海拔高度呈负相关(r =- 0.150、- 0.052,P均< 0.05),与民族未见相关性(r = 0.003,P > 0.05)。结论青海地区人群维生素D水平与TPO水平呈负相关,且维生素D异常率高,男性维生素D水平高于女性。 展开更多
关键词 浆液 免疫血清 血清 维生素D 甲状腺过氧化物酶
脑卒中并发睡眠障碍患者血清细胞因子及神经递质表达的变化研究 预览 被引量:1
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作者 邓介华 温艳 林文 《世界睡眠医学杂志》 2019年第3期269-271,共3页
目的:探讨脑卒中并发睡眠障碍患者血清细胞因子及神经递质表达的变化情况。方法:选取2017年2月至2018年11月攀枝花市中心医院仁和分院收治的脑卒中并发睡眠障碍患者60例为A组,同时期的脑卒中无睡眠障碍患者60例为B组,体检健康者60名为C... 目的:探讨脑卒中并发睡眠障碍患者血清细胞因子及神经递质表达的变化情况。方法:选取2017年2月至2018年11月攀枝花市中心医院仁和分院收治的脑卒中并发睡眠障碍患者60例为A组,同时期的脑卒中无睡眠障碍患者60例为B组,体检健康者60名为C组。比较3组的血清细胞因子及神经递质表达水平,同时比较A组中不同失眠严重指数者的血清细胞因子及神经递质表达水平。结果:A组的血清细胞因子及神经递质表达水平均高于B组及C组,B组则高于C组,且A组中不同失眠严重指数者的血清细胞因子及神经递质表达水平比较,差异有统计学意义(P<0.05)。结论:脑卒中并发睡眠障碍患者血清细胞因子及神经递质均呈现异常表达的状态,且睡眠障碍越为严重的患者其异常程度相对突出。 展开更多
关键词 脑卒中 睡眠障碍 血清 细胞因子 神经递质
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