Calcium carbonate(CaCO3) application is widely used to ameliorate soil acidification. To counteract soil and bacterial community response to CaCO3 application in an acidic paddy soil in southern China, a field experim...Calcium carbonate(CaCO3) application is widely used to ameliorate soil acidification. To counteract soil and bacterial community response to CaCO3 application in an acidic paddy soil in southern China, a field experiment was conducted with four different dosages of CaCO3 addition, 0, 2.25, 4.5 and 7.5 tons/ha, respectively. After one seasonal growth of rice, soil physicochemical properties, soil respiration and bacterial communities were investigated. Results showed that soil p H increased accordingly with increasing dose of CaCO3 addition, and 7.5 tons/ha addition increased soil p H to neutral condition. Moderate dose of CaCO3 application(4.5 tons/ha) significantly increased soil dissolved organic carbon(DOC) and dissolved organic nitrogen(DON) content, enhanced soil respiration, while the excessive CaCO3 application(7.5 tons/ha) decreased these soil properties. High-throughput sequencing results illustrated that moderate dose of CaCO3 application increased the richness and alpha diversity of soil bacterial community. Compared with control, the relative abundance of Anaerolineaceae family belonging to Chloroflexi phylum increased by 38.7%, 35.4% and 24.5% under 2.25, 4.5 and 7.5 tons/ha treatments, respectively.Redundancy analysis(RDA) showed that soil p H was the most important factor shaping soil bacterial community. The results of this study suggest that proper dose of CaCO3 additions to acid paddy soil in southern China could have positive effects on soil properties and bacterial community.展开更多
氮(N)沉降增加带来的土壤酸化问题已经得到广泛的关注,然而土壤酸化是否受到未来降水格局改变的影响研究相对匮乏.本研究基于内蒙古温带典型草原5年(2013—2017年)的N添加(10和40 g N·m^-2·a^-1)和增雨(增雨量80 mm,分2 mm...氮(N)沉降增加带来的土壤酸化问题已经得到广泛的关注,然而土壤酸化是否受到未来降水格局改变的影响研究相对匮乏.本研究基于内蒙古温带典型草原5年(2013—2017年)的N添加(10和40 g N·m^-2·a^-1)和增雨(增雨量80 mm,分2 mm×40次、5 mm×16次、10 mm×8次、20 mm×4次、40 mm×2次5种处理)控制试验分析了水分对N添加后土壤酸化的影响.结果表明:40 g N·m^-2·a^-1 N添加在土壤酸化出现的时间、酸化程度以及酸化随时间的变化速率上均大于10 g N·m^-2·a~(-1)N添加.40 g N·m^-2·a^-1 N添加一年后即在各层土壤中观测到了显著的土壤pH降低,而10 g N·m^-2·a^-1 N添加只有土壤表层(0~5 cm)在N添加一年后出现显著的土壤pH降低,5~10和10~20 cm土层显著的土壤pH降低分别出现在氮添加4年和5年后.氮添加后土壤pH的降低幅度随氮添加年限的延长而增加,40 g N·m^-2·a~(-1)N添加土壤pH随时间的降低速率大于10 g N·m^-2·a^-1N添加.增雨不改变氮添加后土壤pH降低的结果,但中小强度增雨方式(2~20 mm)在干旱年份有缓解10 g N·m^-2·a^-1 N添加处理土壤酸化的趋势,而所有增雨方式在湿润年份均有加剧氮添加(10和40 g N·m^-2·a^-1)后土壤酸化的趋势,尤其是表层土壤,但缓解和加剧的程度均不显著.高强度增雨方式后(10~40 mm)土壤无机氮的淋溶可能是增雨加剧氮添加后土壤酸化的一个重要原因.本研究将为预测草原生态系统对未来氮沉降和降水格局改变的响应提供科学依据.展开更多
基金the Special Fund for AgroScientific Research in the Public Interest(No.201403015)the National Natural Science Foundation of China(No.21677157)the National Key Research and Development Program(Nos.2017YFD0801502 and 2018YFD0800202).
文摘Calcium carbonate(CaCO3) application is widely used to ameliorate soil acidification. To counteract soil and bacterial community response to CaCO3 application in an acidic paddy soil in southern China, a field experiment was conducted with four different dosages of CaCO3 addition, 0, 2.25, 4.5 and 7.5 tons/ha, respectively. After one seasonal growth of rice, soil physicochemical properties, soil respiration and bacterial communities were investigated. Results showed that soil p H increased accordingly with increasing dose of CaCO3 addition, and 7.5 tons/ha addition increased soil p H to neutral condition. Moderate dose of CaCO3 application(4.5 tons/ha) significantly increased soil dissolved organic carbon(DOC) and dissolved organic nitrogen(DON) content, enhanced soil respiration, while the excessive CaCO3 application(7.5 tons/ha) decreased these soil properties. High-throughput sequencing results illustrated that moderate dose of CaCO3 application increased the richness and alpha diversity of soil bacterial community. Compared with control, the relative abundance of Anaerolineaceae family belonging to Chloroflexi phylum increased by 38.7%, 35.4% and 24.5% under 2.25, 4.5 and 7.5 tons/ha treatments, respectively.Redundancy analysis(RDA) showed that soil p H was the most important factor shaping soil bacterial community. The results of this study suggest that proper dose of CaCO3 additions to acid paddy soil in southern China could have positive effects on soil properties and bacterial community.
文摘氮(N)沉降增加带来的土壤酸化问题已经得到广泛的关注,然而土壤酸化是否受到未来降水格局改变的影响研究相对匮乏.本研究基于内蒙古温带典型草原5年(2013—2017年)的N添加(10和40 g N·m^-2·a^-1)和增雨(增雨量80 mm,分2 mm×40次、5 mm×16次、10 mm×8次、20 mm×4次、40 mm×2次5种处理)控制试验分析了水分对N添加后土壤酸化的影响.结果表明:40 g N·m^-2·a^-1 N添加在土壤酸化出现的时间、酸化程度以及酸化随时间的变化速率上均大于10 g N·m^-2·a~(-1)N添加.40 g N·m^-2·a^-1 N添加一年后即在各层土壤中观测到了显著的土壤pH降低,而10 g N·m^-2·a^-1 N添加只有土壤表层(0~5 cm)在N添加一年后出现显著的土壤pH降低,5~10和10~20 cm土层显著的土壤pH降低分别出现在氮添加4年和5年后.氮添加后土壤pH的降低幅度随氮添加年限的延长而增加,40 g N·m^-2·a~(-1)N添加土壤pH随时间的降低速率大于10 g N·m^-2·a^-1N添加.增雨不改变氮添加后土壤pH降低的结果,但中小强度增雨方式(2~20 mm)在干旱年份有缓解10 g N·m^-2·a^-1 N添加处理土壤酸化的趋势,而所有增雨方式在湿润年份均有加剧氮添加(10和40 g N·m^-2·a^-1)后土壤酸化的趋势,尤其是表层土壤,但缓解和加剧的程度均不显著.高强度增雨方式后(10~40 mm)土壤无机氮的淋溶可能是增雨加剧氮添加后土壤酸化的一个重要原因.本研究将为预测草原生态系统对未来氮沉降和降水格局改变的响应提供科学依据.