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Umbelliferone ameliorates renal function in diabetic nephropathy rats through regulating inflammation and TLR/NF-κB pathway
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作者 WANG Han-Qing WANG Sha-Sha +2 位作者 Chiufai Kuok WANG Qi CHENG Xiao-Lan 《中国天然药物:英文版》 SCIE CAS CSCD 2019年第5期346-354,共9页
Diabetic nephropathy (DN) is a leading cause of renal failure, contributing to severe morbidity and mortality in diabetic patients. Umbelliferae (Umb) has been well characterized to exert protective effects in diabete... Diabetic nephropathy (DN) is a leading cause of renal failure, contributing to severe morbidity and mortality in diabetic patients. Umbelliferae (Umb) has been well characterized to exert protective effects in diabetes. However, the action and mechanism of Umb in DN remains unclear. In this work, we studied the effect of Umb in a streptozotocin (STZ)-induced DN rat model and explore its underlying mechanism. DN rats were treated with Umb (20, 40 mg·kg^-1) or irbesartan (15 mg·kg^-1) for 4 weeks. Levels of serum glucose, insulin, blood uric acid, creatinine, triglycerides (TG) and total cholesterol (TC) were measured by commercial assay kits, respectively. Histopathological changes and inflammatory cytokine levels including IL-6, IL-1β and TNF-α in the kidney were also evaluated. Alterations in the expression of podocin, CD2AP and TLR/NF-κB were assessed by western blotting. Our results showed that Umb reduced renal injury in DN rat model, as evidenced by the decrease in blood glucose, 24 h urinary protein, serum creatinine, and blood uric acid. Umb also significantly ameliorated the renal histopathological alteration, and down-regulated the expression of epithclial-to-mesenchymal transition-related molecular markers podocin and CD2AP. Moreover, Umb inhibited TLR2, TLR4, MyD88 expressions, NF-κB activation and considerably reduced levels of other downstream inflammatory molecules (TNF-α, IL-6, IL-1β). These findings indicated that Umb improved renal function through regulating inflammation and TLR/NF-κB pathway, suggesting the potential efficacy of Umb in DN treatment. 展开更多
关键词 DIABETIC NEPHROPATHY UMBELLIFERONE STREPTOZOTOCIN INFLAMMATION TLR/NF-kB pathway
重组人神经生长因子对糖尿病大鼠难愈创面愈合的影响 预览
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作者 王甜甜 程玉芳 徐江平 《中国药理学通报》 CAS CSCD 北大核心 2019年第6期793-796,共4页
目的 研究重组人神经生长因子(recombinant human nerve growth factor,rhNGF)对糖尿病大鼠合并难愈创面的治疗效果。方法 腹腔注射链脲佐菌素(60 mg·kg^-1 )诱导大鼠糖尿病模型,并在大鼠背部造成一圆形烫伤创面,建立糖尿病合并难... 目的 研究重组人神经生长因子(recombinant human nerve growth factor,rhNGF)对糖尿病大鼠合并难愈创面的治疗效果。方法 腹腔注射链脲佐菌素(60 mg·kg^-1 )诱导大鼠糖尿病模型,并在大鼠背部造成一圆形烫伤创面,建立糖尿病合并难愈创面的动物模型。用药组rhNGF(3、6、12 μg·kg ^-1 )肌肉注射给药,每天1次,连续21 d。测定大鼠伤口面积,HE染色和透射电镜检查创面皮肤组织病理形态学变化。结果 rhNGF明显减小糖尿病大鼠烫伤创伤面积( P < 0.05);HE染色结果表明,rhNGF明显促进糖尿病大鼠烫伤创面纤维细胞和毛细血管再生;透射电镜结果表明,rhNGF对糖尿病大鼠烫伤创面皮肤的上皮细胞结构排列、细胞连接以及上皮下胶原等超微结构有明显改善作用。结论 rhNGF对糖尿病大鼠烫伤性难愈创面具有促进愈合的作用。 展开更多
关键词 重组人神经生长因子 链脲佐菌素 糖尿病 大鼠 溃疡 创面愈合
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金钗石斛总生物碱对糖尿病大鼠骨骼肌组织GLUT4表达的影响
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作者 黄琦 廖鑫 +1 位作者 吴芹 石京山 《中国新药杂志》 CAS CSCD 北大核心 2019年第13期1625-1628,共4页
目的:研究金钗石斛总生物碱(Dendrobium nobile Lindl. alkaloids,DNLA)对糖尿病大鼠骨骼肌组织葡萄糖转运蛋白4(GLUT4)表达的影响。方法:高脂高糖饲料喂养SD大鼠6周后,腹腔注射链脲佐菌素(streptozotocin,STZ)制备糖尿病大鼠模型。糖... 目的:研究金钗石斛总生物碱(Dendrobium nobile Lindl. alkaloids,DNLA)对糖尿病大鼠骨骼肌组织葡萄糖转运蛋白4(GLUT4)表达的影响。方法:高脂高糖饲料喂养SD大鼠6周后,腹腔注射链脲佐菌素(streptozotocin,STZ)制备糖尿病大鼠模型。糖尿病大鼠按血糖、体质量随机分为模型组,二甲双胍组(100mg·kg^-1)、DNLA 20 mg·kg-1组、DNLA 40 mg·kg^-1组和DNLA 80 mg·kg^-1组,另设空白对照组。灌胃(ig)给药4周以后,检测大鼠空腹血糖(FPG)、三酰甘油(TG)、总胆固醇(TC)、空腹胰岛素(FINS),计算胰岛素抵抗指数(HOMA-IR),Real time PCR检测各组大鼠骨骼肌组织中GLUT4 mRNA表达,同时制备相同部位骨骼肌病理切片,采用免疫组化法检测其中GLUT4表达。结果:糖尿病大鼠模型FPG,TG,FINS,HOMA-IR明显升高(P <0. 05);骨骼肌组织中GLUT4 mRNA表达明显减少(P <0. 05);免疫组化检测到大鼠骨骼肌组织中GLUT4表达较空白组减少;DNLA 40 mg·kg^-1组、DNLA 80 mg·kg-1组及二甲双胍组FPG,FINS,HOMA-IR较模型组显著降低(P <0. 05);骨骼肌组织中GLUT4 mRNA表达明显增加(P <0. 05);镜下观察免疫组化染色显示骨骼肌组织中GLUT4表达有不同程度增加。结论:DNLA通过减轻糖尿病大鼠胰岛素抵抗、上调骨骼肌组织GLUT4表达而发挥其降血糖作用。 展开更多
关键词 金钗石斛 生物碱 链脲佐菌素 糖尿病 大鼠 葡萄糖转运蛋白4
格列美脲对链脲佐菌素诱导的阿尔茨海默病模型小鼠认知行为的影响 预览
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作者 吕维名 黄樱 +3 位作者 许荣 袁建清 王瑒 宋紫檀 《赣南医学院学报》 2019年第6期549-551,共3页
目的:探讨格列美脲对链脲佐菌素(STZ)诱导阿尔茨海默病(AD)模型小鼠认知行为的影响。方法:将30只昆明小鼠随机分为空白对照组、模型组及格列美脲3组,每组10只,模型组与格列美脲组小鼠侧脑室注射STZ,建立小鼠阿尔茨海默病(AD)模型,格列... 目的:探讨格列美脲对链脲佐菌素(STZ)诱导阿尔茨海默病(AD)模型小鼠认知行为的影响。方法:将30只昆明小鼠随机分为空白对照组、模型组及格列美脲3组,每组10只,模型组与格列美脲组小鼠侧脑室注射STZ,建立小鼠阿尔茨海默病(AD)模型,格列美脲组持续给药30d,采用水迷宫实验,检测格列美脲对STZ-ICV模型小鼠认知行为的影响。结果:Morris水迷宫:定位航行试验中,AD模型组平均逃避潜伏期和路径长度较对照组明显增加,格列美脲治疗组平均逃避潜伏期和路径长度较AD模型组明显减少(P<0.05);空间探索试验中,AD模型组穿越隐匿平台次数较对照组明显减少,格列美脲治疗组穿越隐匿平台次数较AD模型组显著增加(P<0.05)。结论:格列美脲对STZ诱导的AD模型小鼠的学习记忆能力有明显的改善作用。 展开更多
关键词 格列美脲 链脲佐菌素 阿尔茨海默病 认知行为
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Antidiabetic effect of Opuntia dillenii seed oil on streptozotocin-induced diabetic rats
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作者 Mohamed Bouhrim Hayat Ouassou +6 位作者 El Hassania Loukili Mohammed Ramdani Hassane Mekhfi Abderrahim Ziyyat Abdelkhaleq Legssyer Mohammed Aziz Mohamed Bnouham 《亚太热带生物医学杂志:英文版》 CAS 2019年第9期381-388,共8页
Objective: To assess the antidiabetic effect of Opuntia dillenii seed oil on rats with diabetes mellitus. Methods: A rat diabetes model was established by intraperitoneal injection of rats with 50 mg/kg streptozotocin... Objective: To assess the antidiabetic effect of Opuntia dillenii seed oil on rats with diabetes mellitus. Methods: A rat diabetes model was established by intraperitoneal injection of rats with 50 mg/kg streptozotocin. Thirty albino Wistar rats were divided into five groups: the diabetic control group and normal control group were treated only with distilled water, two diabetic groups received 1 and 2 m L/kg of oil per day, respectively, for 30 days and one diabetic group received 2 mg/kg of glibenclamide. In addition, blood glucose was determined weekly. Body weight, average daily food, water intake and urinary volume of each animal were determined before and after the treatment period. After the treatment period, hepatic glycogen was determined using the anthrone reagent, and glycosuria, total cholesterol, triglycerides, alanine aminotransferase, aspartate aminotransferase, urea, creatinine and uric acid were estimated using common clinical diagnostic kits. Results: Oral intake of the oil at 1 and 2 m L/kg for the diabetic animals significantly diminished blood glucose, glycosuria, total cholesterol, triglycerides, alanine aminotransferase, aspartate aminotransferase, urea, creatinine and uric acid, accompanied by a noticeable elevation in the amount of hepatic glycogen in comparison with the diabetic control group. Similarly, Opuntia dillenii seed oil significantly increased the food intake and decreased the urinary volume per day in treated rats of the same groups in comparison with the period before the treatment intervention and attenuated body weight loss in the diabetic rats. Moreover, this effect of the oil was dose dependent. On the other hand, the oil did not affect their need for water. Conclusions: The results show that Opuntia dillenii seed oil has a very important antidiabetic effect on streptozotocin-induced diabetic rats. Hence, we suggest it as a preventive control of diabetes mellitus. 展开更多
关键词 OPUNTIA dillenii SEEDS oil STREPTOZOTOCIN DIABETES Rats BIOCHEMICAL parameters
链脲佐菌素构建糖尿病小鼠视网膜病变模型及评价
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作者 张昊瑞 高鑫 +1 位作者 赵子畅 沈炜 《第二军医大学学报》 CAS CSCD 北大核心 2019年第9期977-983,共7页
目的建立链脲佐菌素(STZ)诱导的糖尿病小鼠视网膜病变模型,研究早期糖尿病小鼠视网膜的病理改变及其发展过程中血管内皮生长因子(VEGF)及其受体(VEGFR1、VEGFR2)在动物模型中的表达情况。方法 6~8周龄C57BL/6J小鼠腹腔连续注射STZ(55 mg... 目的建立链脲佐菌素(STZ)诱导的糖尿病小鼠视网膜病变模型,研究早期糖尿病小鼠视网膜的病理改变及其发展过程中血管内皮生长因子(VEGF)及其受体(VEGFR1、VEGFR2)在动物模型中的表达情况。方法 6~8周龄C57BL/6J小鼠腹腔连续注射STZ(55 mg/kg)5 d,注射后1周时测量空腹血糖浓度,将糖尿病模型建立成功的小鼠与对照组小鼠均喂养5个月。注射后5个月时,通过石蜡切片H-E染色、伊文思蓝灌注造影、视网膜血管网铺片等方法分析糖尿病小鼠视网膜组织形态变化。通过实时定量PCR、蛋白质印迹法分析糖尿病视网膜病变过程中VEGF及其受体VEGFR1、VEGFR2的表达情况。结果与对照组相比,注射后1周、1~5个月时模型组小鼠血糖水平均升高(均高于16.5 mmol/L),差异均有统计学意义(P均<0.01)。注射后5个月时模型组小鼠视网膜全层变薄,感光细胞层、内核层及外核层细胞数量减少,排列紊乱;血管走行迂曲,出现渗漏及渗漏斑;血管内皮细胞数量增加,形态改变,周细胞数量减少,可见无细胞毛细血管、管腔闭塞;VEGF、VEGFR1、VEGFR2的蛋白质和mRNA表达均增加,与对照组相比差异均有统计学意义(P均<0.01)。结论成功构建糖尿病小鼠视网膜病变模型,该模型表明在糖尿病发展5个月后出现增殖性糖尿病视网膜病变,且糖尿病小鼠视网膜中VEGF、VEGFR1和VEGFR2表达水平均上升。 展开更多
关键词 糖尿病视网膜病变 链脲佐菌素 血管内皮生长因子 血管内皮生长因子受体
1型与2型糖尿病大鼠周围神经病变的比较 预览
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作者 杨丹 崔雅忠 +4 位作者 张倩 王超 张改丽 梁晓春 吴群励 《基础医学与临床》 CSCD 2019年第1期27-31,共5页
目的观察链脲佐菌素(STZ)诱导的1型与2型糖尿病大鼠周围神经病变的区别。方法SD大鼠分组,每组各10只,腹腔注射STZ建立1型糖尿病(T1DM)模型;高脂高糖饲养6周后按体质量35mg/kg单次腹腔注射STZ建立2型糖尿病(T2DM)模型;设立正常对照(Con)... 目的观察链脲佐菌素(STZ)诱导的1型与2型糖尿病大鼠周围神经病变的区别。方法SD大鼠分组,每组各10只,腹腔注射STZ建立1型糖尿病(T1DM)模型;高脂高糖饲养6周后按体质量35mg/kg单次腹腔注射STZ建立2型糖尿病(T2DM)模型;设立正常对照(Con)组。检测不同时间点大鼠的体质量、血糖水平。成模8周后,电子VonFrey仪检测机械痛阈值,热水甩尾试验检测甩尾潜伏期,并取坐骨神经进行病理形态学检查。结果成模后第1、8周,与对照组及T2DM组相比,T1DM组大鼠的体质量显著下降(P<0.01);T1DM组与T2DM组的血糖均显著高于对照组(P<0.01);DM组的甩尾潜伏期均较对照组延长(P<0.01),T2DM组较T1DM组延长更显著(P<0.05)。DM组的机械痛阈值均较对照组显著降低(P<0.01),T2DM组较T1DM组降低更明显(P<0.01);DM组大鼠的坐骨神经有髓神经纤维排列紊乱,轴索肿胀或皱缩,髓鞘密度不均匀,空泡变性,部分神经纤维髓鞘脱失;T2DM组的神经损害程度较T1DM严重。结论STZ诱导的2型糖尿病大鼠的周围神经病变程度严重于1型糖尿病大鼠。 展开更多
关键词 糖尿病周围神经病变 链脲佐菌素 机械痛阈 热水甩尾试验 病理形态
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Phloroglucinol,a nutraceutical for IR‐induced cardiac damage in diabetic rats 预览
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作者 B.Pranav Nayak K.R.Ganesha +2 位作者 Nathani Minaz Rema Razdan Sumanta Kumar Goswami 《动物模型与实验医学(英文)》 CSCD 2019年第3期210-216,共7页
Background:Myocardial injury due to ischemia‐reperfusion(IR)is aggravated in diabetes which is associated with oxidative stress.Alleviating oxidative stress via use of antioxidants has been shown to be effective at m... Background:Myocardial injury due to ischemia‐reperfusion(IR)is aggravated in diabetes which is associated with oxidative stress.Alleviating oxidative stress via use of antioxidants has been shown to be effective at minimizing myocardial cell death and improving cardiac function.The aim of the present study was to evaluate the cardioprotective effect of phloroglucinol against myocardial reperfusion injury(MRI)in diabetic rats.Methods:Diabetes was induced in female rats with streptozotocin(50 mg/kg).The diabetic rats were orally treated with phloroglucinol(100 and 200 mg/kg daily for 28 days).After treatment the hearts were isolated and mounted on a Langendorff apparatus.The hearts were subjected to 15 minutes of IR to induce myocardial damage.Cardiac functions including heart rate(HR),resting and developed tension,and rate of change of contraction(+dP/dtmax)were recorded.Cardiac injury biomarkers lactate dehydrogenase(LDH)and creatine kinase(CK‐MB)were measured in the heart perfusate.Levels of the antioxidant enzymes reduced glutathione(GSH)and malondialdehyde(MDA)were measured.Hematoxylin and eosin(H&E)staining was also performed.Results:After IR injury,a decrease in HR and+dP/dtmax in hearts from diabetic rat was seen compared to healthy rat hearts,which was reversed by phloroglucinol treatment.Myocardial infarct size,measured by H&E staining,was increased in diabetic rats compared to healthy rats and an increase in the activity of LDH and CK‐MB in the heart perfusate in diabetic rats was decreased by phloroglucinol treatment.An increase in MDA levels and a decrease in levels of antioxidant enzymes were observed in diabetic rats,which was reversed with phloroglucinol treatment.Conclusion:Phloroglucinol treatment has potential therapeutic promise in the treatment of MRI in diabetes. 展开更多
关键词 Langendroff apparatus myocardial reperfusion injury PHLOROGLUCINOL STREPTOZOTOCIN
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光果甘草提酸废渣萃取物降血糖活性的研究
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作者 朱子博 樊金玲 +3 位作者 程源斌 余颖 马艺萌 喻玲 《食品科技》 CAS 北大核心 2019年第6期233-241,共9页
实验研究光果甘草提取甘草酸后废渣的萃取物(WEG)对链脲佐菌素(STZ)诱导糖尿病小鼠的降血糖活性,探讨WEG对糖尿病小鼠的血糖、血脂、抗氧化能力的影响以及对肾脏、肝脏的保护作用;采用高效液相色谱-二极管阵列检测-质谱联用检测技术(HPL... 实验研究光果甘草提取甘草酸后废渣的萃取物(WEG)对链脲佐菌素(STZ)诱导糖尿病小鼠的降血糖活性,探讨WEG对糖尿病小鼠的血糖、血脂、抗氧化能力的影响以及对肾脏、肝脏的保护作用;采用高效液相色谱-二极管阵列检测-质谱联用检测技术(HPLC-DAD-MS),结合与标准品相对照的方法,初步鉴定萃取物中主要色谱峰的化学结构,并采用HPLC法测定主要成分的含量。WEG高剂量组(150 mg/kg)能够恢复糖尿病小鼠的血糖、肝糖原、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)水平以及超氧化物歧化酶(SOD)活力至正常水平,显著降低丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)水平,提高总蛋白(TP)、白蛋白(ALB)水平,并能显著降低肌酐(Cre)和尿素氮(UREA)水平,降血糖效果与阳性高剂量组(盐酸二甲双胍300 mg/kg)无显著差异。WEG中主要色谱峰成分为光甘草定,含量为6.46%。上述研究结果表明:WEG能够显著改善糖尿病小鼠的糖代谢紊乱,有效调节机体氧化应激水平,减轻高脂血症,修复肝、肾损伤,具有成为治疗糖尿病的新型保健品或功能食品的潜在应用价值。 展开更多
关键词 光果甘草 链脲佐菌素 糖尿病 光甘草定
Overexpression of steroid receptor coactivators alleviates hyperglycemia-induced endothelial cell injury in rats through activating the PI3K/Akt pathway
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作者 Xiao-juan Quan Chun-lian Liang +2 位作者 Ming-zhu Sun Lin Zhang Xiu-li Li 《中国药理学报:英文版》 SCIE CAS CSCD 2019年第5期648-657,共10页
Hyperglycemia is a major factor in vascular endothelial injury that finally leads to a cardiovascular event. Steroid receptor coactivators (SRCs) are a group of non-DNA binding proteins that induce structural changes ... Hyperglycemia is a major factor in vascular endothelial injury that finally leads to a cardiovascular event. Steroid receptor coactivators (SRCs) are a group of non-DNA binding proteins that induce structural changes in steroid receptors (nuclear receptors) critical for transcriptional activation. SRCs, namely, SRC-1, SRC-2, and SRC-3, are implicated in the regulation of vascular homeostasis. In this study we investigate the role of SRCs in hyperglycemia-induced endothelial injury. Aortic endothelial cells were prepared from normal and diabetic rats, respectively. Diabetic rats were prepared by injection of streptozotocin (50 mg/kg, i.p.). The expression levels of SRC-1 and SRC-3 were signi?cantly decreased in endothelial cells from the diabetic rats. Similar phenomenon was also observed in aortic endothelial cells from the normal rats treated with a high glucose (25 mM) for 4 h or 8 h. The expression levels of SRC-2 were little affected by hyperglycemia. Overexpression of SRC-1 and SRC-3 in high glucose-treated endothelial cells significantly increased the cell viability, suspended cell senescence, and inhibited cell apoptosis compared with the control cells. We further showed that overexpression of SRC-1 and SRC-3 markedly suppressed endothelial injury through restoring nitric oxide production, upregulating the expression of antioxidant enzymes (SOD, GPX, and CAT), and activating the PI3K/Akt pathway. The beneficial effects of SRC-1 and SRC-3 overexpression were blocked by treatment with the PI3K inhibitor LY294002 (10 mM) or with the Akt inhibitor MK-2206 (100 nM). In conclusion, hyperglycemia decreased SRC-1 and SRC-3 expression levels in rat aortic endothelial cells. SRC-1 and SRC-3 overexpression might protect against endothelial injury via inhibition of oxidative stress and activation of PI3K/Akt pathway. 展开更多
关键词 DIABETICS STREPTOZOTOCIN HYPERGLYCEMIA endothelial cells STEROID receptor COACTIVATORS CELL senescence CELL apoptosis oxidative stress PI3K/Akt pathway LY294002 MK-2206
没食子酸氧钒对STZ诱导的糖尿病小鼠的降糖作用研究 预览
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作者 廉亚楠 高金波 +3 位作者 赵胜男 张云杰 彭翔 侯艳秋 《黑龙江大学自然科学学报》 CAS 2019年第3期335-343,共9页
为了探究没食子酸氧钒对STZ诱导的糖尿病小鼠的降糖作用,采用链脲佐菌素(STZ)建立糖尿病小鼠模型。实验动物分为正常组、模型组、阳性组、GAOV高、中、低剂量组以及VOSO4高、中、低剂量组,造模成功后灌胃28d,监测糖尿病小鼠饮食量、饮... 为了探究没食子酸氧钒对STZ诱导的糖尿病小鼠的降糖作用,采用链脲佐菌素(STZ)建立糖尿病小鼠模型。实验动物分为正常组、模型组、阳性组、GAOV高、中、低剂量组以及VOSO4高、中、低剂量组,造模成功后灌胃28d,监测糖尿病小鼠饮食量、饮水量、体重和FBG的变化,测定小鼠血清生化指标(UREA、Cr、ALB、ALT)和血脂四项指标(CHO、TG、LDL-C、HDL-C),计算胰腺、肝、肾、脾等脏器指数,采用SPSS19.0分析软件对实验数据进行单因素方差分析。结果显示,与模型组相比,GAOV组小鼠体重、饮食饮水量、FBG及各血清生化指标、血脂四项指标变化均存在显著性差异(P<0.05)。其中,GAOV中剂量组与模型组有极显著性差异(P<0.01),与阳性组无显著性差异(P>0.05)。综上所述,没食子酸氧钒对STZ诱导的糖尿病小鼠有较好的体内降糖活性,且降糖作用起效快、效果好,为糖尿病药物的开发提供了新的方向。 展开更多
关键词 没食子酸 氧钒配合物 链脲佐菌素 糖尿病小鼠 降糖活性
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Potential of phloroglucinol to improve erectile dysfunction associated with streptozotocin-induced diabetes in rats
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作者 Syed Kashif Rema Razdan Ramanaiah Illuri 《结合医学学报:英文版》 CAS CSCD 2019年第4期282-287,共6页
Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the e... Objective: Diabetes is a common metabolic disease with several complications in its patients. Often, people living with diabetes develop erectile dysfunction (ED). The primary aim of this work was to investigate the effect of phloroglucinol in diabetes-induced ED in rats. Methods: Male Wistar rats were given 52 mg/kg of streptozotocin, by intraperitoneal injection, to induce diabetes and ED. Subsequently, animals were grouped into three groups: group 1, diabetic control;group 2, low-dose phloroglucinol (150 mg/kg body weight);and group 3, high-dose phloroglucinol (250 mg/kg body weight). A group of six normal rats served as a normal control. The rats were treated with phloroglucinol for six weeks and then were assessed for treatment effects. Sexual behavior, glycosylated hemoglobin Ale (HbAlc) values, serum testosterone, serum nitric oxide (NO), blood pressure and sperm count were measured after the end of treatment. Results: After a 6-week treatment period, the high dose of phloroglucinol significantly decreased HbAlc values in diabetic rats. Rats treated with phloroglucinol had increased serum testosterone, NO and sperm count. Animals treated with 250 mg/kg phloroglucinol performed similar to normal rats in the sexual behavioral study, suggesting the reversal of complications of ED. Conversely, a decrease in the blood pressure in treated groups was observed. Conclusion: The results highlight the protective effect of phloroglucinol in diabetes-induced ED in rats warranting further studies. 展开更多
关键词 DIABETES STREPTOZOTOCIN Erectile DYSFUNCTION PHLOROGLUCINOL Glycated HEMOGLOBIN A
Diabetic neuropathy research: from mouse models to targets for treatment 预览
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作者 Vuong M. Pham Shinji Matsumura +2 位作者 Tayo Katano Nobuo Funatsu Seiji Ito 《中国神经再生研究:英文版》 SCIE CAS CSCD 2019年第11期1870-1879,共10页
Diabetic neuropathy is one of the most serious complications of diabetes, and its increase shows no sign of stopping. Furthermore, current clinical treatments do not yet approach the best effectiveness. Thus, the deve... Diabetic neuropathy is one of the most serious complications of diabetes, and its increase shows no sign of stopping. Furthermore, current clinical treatments do not yet approach the best effectiveness. Thus, the development of better strategies for treating diabetic neuropathy is an urgent matter. In this review, we first discuss the advantages and disadvantages of some major mouse models of diabetic neuropathy and then address the targets for mechanism-based treatment that have been studied. We also introduce our studies on each part. Using stem cells as a source of neurotrophic factors to target extrinsic factors of diabetic neuropathy, we found that they present a promising treatment. 展开更多
关键词 brain derived NEUROTROPHIC FACTOR DIABETES extrinsic FACTORS NERVE growth FACTOR NERVE regeneration NEUROTROPHIC FACTORS NON-OBESE type 2 DIABETES phosphatase and tensin homolog stem cell streptozotocin
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肉桂多酚对链脲佐菌素致糖尿病小鼠的保护作用 预览 被引量:1
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作者 廖作庄 徐灵源 +2 位作者 王金妮 谭宏棣 韦丽美 《西安交通大学学报:医学版》 CAS CSCD 北大核心 2019年第1期162-166,共5页
目的探讨肉桂多酚对链脲佐菌素(streptozotocin,STZ)致糖尿病小鼠的保护作用及其分子机制。方法建立单剂量STZ240mg/kg诱导糖尿病小鼠模型,随机分成5组:STZ模型对照组、二甲双胍阳性组(0.3g/kg)及肉桂多酚低、中、高剂量组(0.3、0.6、1.... 目的探讨肉桂多酚对链脲佐菌素(streptozotocin,STZ)致糖尿病小鼠的保护作用及其分子机制。方法建立单剂量STZ240mg/kg诱导糖尿病小鼠模型,随机分成5组:STZ模型对照组、二甲双胍阳性组(0.3g/kg)及肉桂多酚低、中、高剂量组(0.3、0.6、1.2g/kg),持续每天灌胃,共14d。并设置正常对照组。末次给药后,血糖试纸测定空腹血糖(fasting blood-glucose,FBG),生化法测定血清胰淀粉酶含量,酶联免疫吸附测定法(enzyme-linked immunosorbent assay,ELISA)测定血清胰岛素和胰高血糖素浓度,免疫组化法检测胰岛细胞中胰岛素阳性细胞数量,蛋白免疫印迹(Western blot)法检测胰脏细胞中蛋白激酶B(AKT)及其磷酸化水平。结果与STZ模型对照组小鼠比较,肉桂多酚干预组小鼠的血糖明显降低(P<0.05),血清胰岛素增加而胰高血糖素降低(P<0.05),胰岛细胞中胰岛素阳性细胞显著增加(P<0.05),胰脏细胞中蛋白激酶B(AKT)及其磷酸化水平同时增加(P<0.05),且表现为一定的剂量依赖性。结论肉桂多酚对STZ所致糖尿病小鼠胰脏损害表现出有效的细胞保护作用,其分子机制可能通过调节胰岛细胞AKT信号通路促进胰岛β细胞分泌胰岛,从而发挥降血糖药理活性。 展开更多
关键词 肉桂多酚 链脲佐菌素 糖尿病 胰岛素 AKT通路
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Galangin ameliorates changes of membrane-bound enzymes in rats with streptozotocin-induced hyperglycemia
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作者 Amal A.Aloud Chinnadurai Veeramani +2 位作者 Chandramohan Govindasamy Mohammed A.Alsaif Khalid S.AlNumair 《亚太热带生物医学杂志:英文版》 CAS 2019年第7期284-290,共7页
Objective: To assess the protective effect of galangin on membrane bound enzymes in rats with streptozotocin-induced diabetes. Methods: A single low dose of streptozotocin was injected to adult male albino rats to ind... Objective: To assess the protective effect of galangin on membrane bound enzymes in rats with streptozotocin-induced diabetes. Methods: A single low dose of streptozotocin was injected to adult male albino rats to induce hyperglycemia. Galangin(8 mg/kg) or glibenclamide 600 μg/kg as a standard drug was given orally once daily for 45 days by gavage. Membrane-bound adenosine triphosphatases were determined including total ATPase, sodium-potassium-ATPase, calcium-ATPase and magnesium-ATPase in erythrocytes and tissues(kidney, liver, and heart). Results: The levels of total ATPases, sodium-potassium-ATPase, calcium-ATPase and magnesium-ATPase in erythrocytes and tissues were significantly altered in diabetic rats as compared to that in normal rats. After 45 days of treatment with galangin or glibenclamide, the levels of these enzymes were similar to that of normal control rats. Conclusions: Oral administration of galangin or glibenclamide can improve activities of these membrane-bound ATPases towards normal levels. Mechanism of galangin needs to be further explored in future. 展开更多
关键词 STREPTOZOTOCIN HYPERGLYCEMIA Sodium-potassium-ATPase Calcium-ATPase Magnesium-ATPase GALANGIN
两种方法建立的妊娠期糖尿病小鼠模型 预览
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作者 郝秋云 宋成文 《世界最新医学信息文摘(电子版)》 2019年第6期185-186,共2页
目的比较链脲佐菌素一次高剂量注射法与多次低剂量注射法建立妊娠期糖尿病小鼠模型的成模率及稳定性。方法将30只C57孕鼠随机分为对照组(l0只)和实验组(STZ 50mg/kg组、30mg/kg组,每组10只)。对照组一次性腹腔注射等剂量的柠檬酸缓冲液... 目的比较链脲佐菌素一次高剂量注射法与多次低剂量注射法建立妊娠期糖尿病小鼠模型的成模率及稳定性。方法将30只C57孕鼠随机分为对照组(l0只)和实验组(STZ 50mg/kg组、30mg/kg组,每组10只)。对照组一次性腹腔注射等剂量的柠檬酸缓冲液,实验组分别予一次性腹腔注射现配链脲佐菌素溶液50mg/kg和30mg/kg间隔24h腹腔注射,共注射3次。之后均给予普通饲料喂养,自由进水。分别于妊娠第0、6、9、19天测空腹血糖与体重,并观察饮水量与尿量变化,比较孕鼠成模情况。结果 50mg/kg链脲佐菌素组成模率为50%,30mg/kg组成膜率70%,空腹血糖与对照组相比较其高血糖状态持续时间长,且体重增加明显下降,差异具有统计学意义(P<0.05)。结论链脲佐菌素30mg/kg,间隔24h腹腔注射,共注射3次是建立妊娠期糖尿病小鼠模型相对较好的方法,具有较好的稳定性。 展开更多
关键词 链脲佐菌素 妊娠期糖尿病 小鼠 模型
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经典Wnt/β catenin/TCF7L2信号通路在1型糖尿病心肌病中的作用 预览
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作者 邱晓霞 李逸朗 +4 位作者 梁关凤 张贵平 罗健东 袁文常 侯宁 《中国药理学通报》 CAS CSCD 北大核心 2019年第8期1104-1109,共6页
目的 探讨Wnt/β-catenin/TCF7L2信号通路在1型糖尿病小鼠心肌病中的作用。方法利用7~8周龄♂C57BL/6小鼠腹腔注射链脲佐菌素,建立1型糖尿病模型,糖尿病小鼠成模4周后,随机分为糖尿病组、β-catenin通路抑制剂iCRT14(2.5、5 mg·kg^... 目的 探讨Wnt/β-catenin/TCF7L2信号通路在1型糖尿病小鼠心肌病中的作用。方法利用7~8周龄♂C57BL/6小鼠腹腔注射链脲佐菌素,建立1型糖尿病模型,糖尿病小鼠成模4周后,随机分为糖尿病组、β-catenin通路抑制剂iCRT14(2.5、5 mg·kg^-1)组。连续腹腔给药8周后,HE染色检测心脏细胞形态;免疫组化及Western blot检测β-catenin、TCF7L2蛋白表达;荧光定量PCR检测β-catenin、Tcf7l2、Nppa、c-Myc mRNA表达。结果糖尿病组心肌细胞排列相对紊乱、细胞核大小不规则;Western blot和免疫组化结果显示,糖尿病组小鼠心脏β-catenin、TCF7L2表达升高,心肌细胞核内β-catenin、TCF7L2表达明显增多;qPCR显示,β-catenin/TCF7L2通路下游基因c-Myc、心肌肥大基因Nppa表达上调。iCRT14组小鼠心肌细胞排列相对规则,形态趋于正常;β-catenin、TCF7L2蛋白表达降低,核内表达减少;肥大基因Nppa、下游基因c-Myc mRNA表达明显减低。结论 Wnt/β-catenin/TCF7L2通路活化在糖尿病心肌病发生、发展中发挥重要作用,β-catenin抑制剂能明显减缓1型糖尿病小鼠心肌病的进程。 展开更多
关键词 糖尿病 链脲佐菌素 心肌肥厚 Wnt/β-catenin/TCF7L2信号通路 iCRT14 c-Myc Nppa
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链脲佐菌素诱导1型糖尿病大鼠模型的最佳禁食时间与最优剂量
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作者 叶桐江 郑博文 +1 位作者 赵琳 张选奋 《兰州大学学报:医学版》 CAS 2019年第2期52-55,共4页
目的通过观察不同禁食时间及不同剂量链脲佐菌素(STZ)对建立1型糖尿病(T1DM)大鼠模型的影响,探讨STZ建立T1DM动物模型的最佳禁食时间及最优剂量。方法选取清洁级雌性SD大鼠105只,随机分为7组,正常对照组(N组)及实验组(A、B、C、D、E、F... 目的通过观察不同禁食时间及不同剂量链脲佐菌素(STZ)对建立1型糖尿病(T1DM)大鼠模型的影响,探讨STZ建立T1DM动物模型的最佳禁食时间及最优剂量。方法选取清洁级雌性SD大鼠105只,随机分为7组,正常对照组(N组)及实验组(A、B、C、D、E、F组),每组15只。A组禁食12 h,STZ 50 mg/kg;B组禁食16 h,STZ 50 mg/kg;C组禁食20 h,STZ 50 mg/kg;D组禁食24 h,STZ 50 mg/kg;E组禁食20 h,STZ 60 mg/kg;F组禁食20 h,STZ 70 mg/kg;N组禁食20 h,等体积的柠檬酸盐缓冲液。注射STZ后第3天的随机血糖值>16.7 mmo1/L,并表现出明显的多饮、多食及多尿者判定为T1DM大鼠模型造模成功。结果 A组成模率60.00%,死亡率0;B组成模率66.70%,死亡率6.67%;C组成模率93.33%,死亡率6.67%;D组成模率93.33%,死亡率26.67%;E组成模率100.00%,死亡率26.67%;F组成模率93.33%,死亡率40.00%。结论禁食时间20 h,腹腔一次性注射STZ 50 mg/kg为建立T1DM大鼠模型的最佳禁食时间和最优剂量。 展开更多
关键词 链脲佐菌素 1型糖尿病 大鼠 成模率 死亡率
链脲佐菌素对糖尿病小鼠性腺功能的影响 预览
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作者 杨文娟 张嘉园 +3 位作者 马养民 龚频 王兰 陈瑶 《陕西科技大学学报》 CAS 2019年第5期63-66,71共5页
研究了链脲佐菌素(streptozotocin,STZ)对糖尿病小鼠性腺功能的影响.取性功能正常雄性昆明小鼠70只,随机分为对照组(10只)和模型组(60只),模型组腹腔注射STZ(75mg/kg),连续注射2d,1次/d,对照组腹腔注射柠檬酸缓冲液.末次注射72h后测定... 研究了链脲佐菌素(streptozotocin,STZ)对糖尿病小鼠性腺功能的影响.取性功能正常雄性昆明小鼠70只,随机分为对照组(10只)和模型组(60只),模型组腹腔注射STZ(75mg/kg),连续注射2d,1次/d,对照组腹腔注射柠檬酸缓冲液.末次注射72h后测定空腹血糖(fasting blood glucose,FBG),取血糖值≥11.1mmol/L模型组小鼠进行后续试验,分别于7d、14d、21d、28d测定空腹血糖,并进行交配实验,监测跨骑次数和交配次数,于28d交配实验后处死小鼠,测定血清睾酮水平.结果表明,与对照组相比,模型组小鼠在注射STZ后7d、14d、21d、28d,血糖均明显升高(P<0.05);且7d、14d、21d、28d交配试验跨骑次数及交配次数均明显减少(P<0.05,P<0.01),28d小鼠血清睾酮水平显著降低(P<0.01).因此,STZ小剂量多次腹腔注射可成功诱导糖尿病小鼠性腺功能减退. 展开更多
关键词 链脲佐菌素 性腺功能减退症 糖尿病
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C57BL/6J糖尿病小鼠模型的优化研究
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作者 张吟 黄丹丹 +2 位作者 张淑芬 许秋霞 李营营 《中国现代应用药学》 CAS CSCD 北大核心 2019年第6期655-660,共6页
目的优化链脲佐菌素(streptozotocin,STZ)诱导的C57BL/6J糖尿病小鼠模型。方法采用单用不同剂量STZ(180 mg·kg-1单次给药和275 mg·kg-1均分5次,每次55 mg·kg-1,连续5 d)或4周高脂饮食联合不同剂量STZ(50 mg·kg-1单... 目的优化链脲佐菌素(streptozotocin,STZ)诱导的C57BL/6J糖尿病小鼠模型。方法采用单用不同剂量STZ(180 mg·kg-1单次给药和275 mg·kg-1均分5次,每次55 mg·kg-1,连续5 d)或4周高脂饮食联合不同剂量STZ(50 mg·kg-1单次给药;100 mg·kg-1单次给药;150 mg·kg-1单次给药;200 mg·kg-1均分2次给药,间隔72 h),建立糖尿病小鼠模型,检测各组小鼠空腹血糖、体质量、日饮水量及日进食量,比较各组造模成功率及稳定性。结果STZ 180 mg·kg-1单次给药、高脂饮食4周+STZ 150 mg·kg-1单次给药、高脂饮食4周+STZ 200 mg·kg-1均分2次给药得到的糖尿病小鼠模型,其高血糖的持续时间较长且稳定。结论STZ 180 mg·kg-1单次给药是较为理想的1型糖尿病模型;4周高脂饮食联合STZ 150mg·kg-1单次给药或200 mg·kg-1均分2次给药均是较为理想的2型糖尿病模型。 展开更多
关键词 糖尿病模型 小鼠 链脲佐菌素 C57BL/6J小鼠 模型优选
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