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Reactive oxygen species mediates a metabolic memory of high glucose stress signaling in bovine retinal pericytes
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作者 Li-Wei Zhang Han Zhao Bai-Hua Chen 《国际眼科杂志:英文版》 SCIE CAS 2019年第7期1067-1074,共8页
AIM: To investigate the role of reactive oxygen species (ROS) and antioxidant mechanism underlying the metabolic memory of bovine retinal pericytes (BRPs) induced by high glucose.METHODS: Effects of high glucose level... AIM: To investigate the role of reactive oxygen species (ROS) and antioxidant mechanism underlying the metabolic memory of bovine retinal pericytes (BRPs) induced by high glucose.METHODS: Effects of high glucose levels and culture time on BRPs viability were evaluated by CCK-8. BRPs were grown in high-glucose media (30 mmol/L) for 4d followed by culture in normal glucose condition (5.6 mmol/L) for 4d in an experimental group. In contrast, in negative and positive control groups, BRPs were grown in either normal-glucose media or high-glucose media for 8d, respectively. The ROS levels, apoptosis, the expression and activity of manganese superoxide dismutase (MnSOD) in BRPs, as well as the protective effect of adeno-associated viral (AAV)-mediated over expression of MnSOD were determined separately by DCHFA, ELISA and Western blot.RESULTS: Comparing the result of cells apoptosis, activity and protein expression of MnSOD and caspase-3, the cell culture system that exposed in sequence in 30 mmol/L and normal glucose for 4d was demonstrated as a suitable model of metabolic memory. Furthermore, delivery of antioxidant gene MnSOD can decrease BRPs apoptosis, reduce activated caspase-3, and reverse hyperglycemic memory by reducing the ROS of mitochondria.CONCLUSION: Increased ROS levels and decreased MnSOD levels may play important roles in pericyte loss of diabetic retinopathy. BRPs cultured in high glucose for 4d followed by normal glucose for 4d could be an appropriate model of metabolic memory. rAAV-MnSOD gene therapy provides a promising strategy to inhibit this blinding disease. 展开更多
关键词 diabetic RETINOPATHY metabolic memory manganese superoxide DISMUTASE molecular THERAPEUTICS reactive oxygen species
Visual dual chemodynamic/photothermal therapeutic nanoplatform based on superoxide dismutase plus Prussian blue
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作者 Shan Lei Jinxing Chen +2 位作者 Kun Zeng Mozhen Wang Xuewu Ge 《纳米研究:英文版》 SCIE EI CAS CSCD 2019年第5期1071-1082,共12页
Enzyme-based anticancer therapy is more attractive for the less side effect than conventional chemotherapy.However,the poor stability and low membrane permeability of enzymes during the intracellular delivery are cons... Enzyme-based anticancer therapy is more attractive for the less side effect than conventional chemotherapy.However,the poor stability and low membrane permeability of enzymes during the intracellular delivery are constraints for its practical applications.In this work,we synthesized novel near-infrared (NIR)-responsive core-shell-structured Prussian blue@fibrous SiO2 (PBFS) nanoparticles as the carrier of superoxide dismutase (SOD) and a glutathione (GSH)-activated Fenton reagent (DiFe).The PBFS nanoparticles are further modified with aGSH-responsive cationic polymer (poly(2-(acryloyloxy)-N,N-dimethyl-N-(4-(((2-((2-(((4-methyl-2-oxo-2H-chromen-7-yl)carbamoyl)oxy)ethyl)disulfaneyl)ethoxy)carbonyl)amino)benzyl)ethan-1-aminium,PSS) containing disulfide bonds and fluorophores.After SOD and DiFe are loaded on the PBFS-PSS nanoparticles,dual chemodynamic/photothermal therapeutic nanoparticulate systems (PBFS-PSS/DiFe/SOD) are obtained.In vitro experiments show that PBFS-PSS/DiFe/SOD nanoparticles have good biocompatibility and can be tracked under fluorescence microscope during the intracellular delivery process in MCF-7 tumor cells due to the GSH-activated release of fluorophores.They also exhibit high efficiency in NIR photothermal conversion and GSH-activated Fenton reaction in tumor cells,thus achieving high-efficient killing effect of tumor cells based on the combination of photothermal and chemodynamic therapeutic performance (PTT and CDT).This work offers a novel pathway to construct a visual multifunctional nanomedicine platform for future cancer therapy. 展开更多
关键词 copper-zinc superoxide DISMUTASE Fenton REAGENT Prussian blue NEAR-INFRARED (NIR) PHOTOTHERMAL therapy fluorescence
Enhanced Peroxymonosulfate Activation by NixCo1-xOOH for Efficient Catalytic Oxidation of Organic Pollutants
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作者 LYU Cong LI Yicheng +3 位作者 FANG Chen FENG Wei SUN Wentian ZHANG Qihui 《高等学校化学研究:英文版》 SCIE CAS CSCD 2019年第3期440-448,共9页
Peroxymonosulfate(PMS) has received increasing attention as viable technology for recalcitrant organics removal from polluted waters. Although promising, alternative heterogeneous catalysts with stable structure, stro... Peroxymonosulfate(PMS) has received increasing attention as viable technology for recalcitrant organics removal from polluted waters. Although promising, alternative heterogeneous catalysts with stable structure, strong hydrophilicity, environmental friendliness and excellent catalytic performance are highly desirable to facilitate the wide industrialization of PMS. In this work, Ni doped CoOOH catalyst was employed as PMS activator. Ni dopant had a significant influence on the morphology, structure and catalytic performance of CoOOH. NiojCoo.gOOH exhibited the best catalytic performance. Reaction rate ofNio.2Coo.8OOH was 2, 4, and 4.4 times that of CoOOH, CoFe2O4 and CO3O4, respectively. Moreover, Ni。2C00.8OOH/PMS system had potential application to organic pollutants and displayed a great catalytic activity over a broader pH value(e.g., 4-10). More importantly, Ni doping accelerated the transfonnation of Co(Ⅲ) and Co(Ⅱ) and formed active species CoOH^+ and NiOH^+ which were responsible to the enhancement of PMS activation.OH, SO4^-:O2^- and 1^O2 were detected, indicating both non-radical and radical processes in the Nio.2Coo.8OOH/PMS system. These findings provide a promising alternative to mixed-metal oxyhydroxides catalysts for PMS activation, demonstrating a great potential in environmental remediation and wastewater treatment. 展开更多
关键词 Ni DOPANT Sulfate RADICAL Superoxide RADICAL SINGLET oxygen Electron transfer
Cellular and Molecular Mechanisms Underlying Arterial Baroreceptor Remodeling in Cardiovascular Diseases and Diabetes
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作者 Huiyin Tu Dongze Zhang Yu-Long Li 《神经科学通报:英文版》 SCIE CAS CSCD 2019年第1期98-112,共15页
Clinical trials and animal experimental studies have demonstrated an association of arterial baroreflex impairment with the prognosis and mortality of cardiovascular diseases and diabetes. As a primary part of the art... Clinical trials and animal experimental studies have demonstrated an association of arterial baroreflex impairment with the prognosis and mortality of cardiovascular diseases and diabetes. As a primary part of the arterial baroreflex arc, the pressure sensitivity of arterial baroreceptors is blunted and involved in arterial baroreflex dysfunction in cardiovascular diseases and diabetes.Changes in the arterial vascular walls, mechanosensitive ion channels, and voltage-gated ion channels contribute to the attenuation of arterial baroreceptor sensitivity. Some endogenous substances(such as angiotensin II and superoxide anion) can modulate these morphological and functional alterations through intracellular signaling pathways in impaired arterial baroreceptors. Arterial baroreceptors can be considered as a potential therapeutic target to improve the prognosis of patients with cardiovascular diseases and diabetes. 展开更多
关键词 Cardiovascular disease DIABETES BAROREFLEX BARORECEPTOR Vascular wall MECHANOSENSITIVE ION CHANNELS Voltage-gated ION CHANNELS Angiotensin Superoxide Nuclear factor-kappa B
Ln2Zr2O7 compounds (Ln=La, Pr, Sm, Y) with varied rare earth A sites for low temperature oxidative coupling of methane
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作者 Xiuzhong Fang Lianghui Xia +7 位作者 Liang Peng Yuan Luo Junwei Xu Luoji Xu Xianglan Xu Wenming Liu Renyang Zheng Xiang Wang 《中国化学快报:英文版》 SCIE CAS CSCD 2019年第6期1141-1146,共6页
With the objective to develop catalysts having application potential for oxidative coupling of methane (OCM) at relatively lower temperature. A series of Ln2Zr2O7 compounds with varied rare earth A sites have been pre... With the objective to develop catalysts having application potential for oxidative coupling of methane (OCM) at relatively lower temperature. A series of Ln2Zr2O7 compounds with varied rare earth A sites have been prepared by a co-precipitation method. XRD and Raman have proved that pure Ln2Zr2O7 compounds have been successfully prepared for all the catalysts. By decreasing the rA/rB ratio, their crystalline structure transform from an ordered pyrochlore (La2Zr2O7) to a less ordered pyrochlore (Pr2Zr2O7 and Sm2Zr2O7) and eventually to a defective cubic fluorite phase (Y2Zr2O7). H2-TPR, O2-TPD and XPS have testified that the amount of surface active O2-species follows the order of La2Zr2O7>Pr2Zr2O7>Sm2Zr2O7 > Y2Zr2O7, which is well consistent with the reaction performance, indicating that the abundance of surface O2- sites is a critical factor influencing the reaction performance. CO2-TPD has demonstrated that a better catalyst generally possesses a larger amount of surface moderate alkaline sites, which is another factor to affect the reaction performance. It is concluded that the concerted interaction between the two types of surface active sites controls the reaction performance of the Ln2Zr2O7 catalysts. In comparison with the state-of-the art Mn/Na2WO4/SiO2, La2Zr2O7, the best catalyst, exhibits much improved reaction performance below 750 ℃. 展开更多
关键词 PYROCHLORE COMPOUNDS Low temperature OCM A site SUBSTITUTION effects SURFACE superoxide SITES SURFACE alkaline SITES
Thai pigmented rice bran extracts inhibit production of superoxide, nitric oxide radicals and inducible nitric oxide synthase in cellular models
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作者 Kedsara Junmarkho Pintusorn Hansakul 《亚太热带生物医学杂志:英文版》 CAS 2019年第7期291-298,共8页
Objective:To study the inhibitory effect of rice bran extracts of Thai black Kam Muang and red Hawm Dawk Mali Deang on oxidative stress factors including superoxide(O2·-),nitric oxide(NO·),and inducible nitr... Objective:To study the inhibitory effect of rice bran extracts of Thai black Kam Muang and red Hawm Dawk Mali Deang on oxidative stress factors including superoxide(O2·-),nitric oxide(NO·),and inducible nitric oxide synthase(iNOS).Methods:Bran extracts(40%ethanol)of Kam Muang and Hawm Dawk Mali Deang were obtained and evaluated for in vitro 2-2′-azino-di-(3-ethylbenzthiazoline sulfonate)(ABTS)and NO·scavenging activity.Their inhibitory effects on cellular O2·-and NO·were measured in phorbol 12-myristate 13-acetate-stimulated neutrophil-like HL-60 cells and lipopolysaccharidestimulated RAW264.7 macrophages,respectively,and their viability was monitored using the MTT assay.The effect on iNOS expression was also assessed by the Western blotting assay.Total contents of phenolics,flavonoids,and subtypes were also determined.Results:Hawm Dawk Mali Deang exhibited about 3.5-fold greater cellular O2·-inhibitory activity than Kam Muang[EC50 values of(23.57±4.54)and(81.98±1.45)μg/mL,respectively]in phorbol 12-myristate 13-acetate-stimulated HL-60 cells.Hawm Dawk Mali Deang exhibited about 2-fold higher in vitro ABTS·+and NO·scavenging activity than Kam Muang,but it exerted cellular NO·inhibitory activity of only about 26%(undetermined EC50 value)in lipopolysaccharide-stimulated RAW264.7 cells.Conversely,Kam Muang exerted potent cellular NO·inhibitory activity[EC50 value:(281.13±59.18)μg/mL]and dose-dependently decreased iNOS levels.No cytotoxicity of both extracts was detected in both cell types.As for corresponding contents,Hawm Dawk Mali Deang contained higher contents of phenolics and flavonoids than Kam Muang.Moreover,Kam Muang and Hawm Dawk Mali Deang had a high content of total anthocyanins[(14.73±0.52)mg C3GE/g of extract]and total proanthocyanidins[(115.13±1.47)mg CE/g of extract],respectively.Conclusions:Based on these data,bran extracts of Thai black Kam Muang and red rice Hawm Dawk Mali Deang can help lower oxidative stress and inflammation attributed partly to O2·-and NO·. 展开更多
关键词 PIGMENTED rice BRAN EXTRACTS Nitric oxide INHIBITORY ACTIVITY Superoxide radical INHIBITORY ACTIVITY iNOS
Tinospora cordifolia attenuates antipsychotic drug induced hyperprolactinemia in Wistar rats
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作者 Prashant Tiwari Sunil Kumar Dubey Pratap Kumar Sahu 《亚太生殖杂志(英文版)》 2019年第3期132-140,共9页
Objective: To evaluate the anti-hyperprolactinemic effect of methanolic extract of Tinospora cordifolia against antipsychotic/neuroleptic drug induced hyperprolactinemia. Methods: A total of 48 Wistar albino rats were... Objective: To evaluate the anti-hyperprolactinemic effect of methanolic extract of Tinospora cordifolia against antipsychotic/neuroleptic drug induced hyperprolactinemia. Methods: A total of 48 Wistar albino rats were chosen in the study. To induce hyperprolactinemia, haloperidol at 5 mg/kg/day was intraperitoneally administered for 16 continuous days and sulpiride at 20 mg/kg/day was administered intraperitoneally for 28 continuous days. Methanolic extract of Tinospora cordifolia at 200 mg/kg/day and 400 mg/kg/day were administered orally 30 min before administration of haloperidol and sulpiride for 16 and 28 days, respectively. Then, we had evaluated prolactin, dopamine and antioxidant status in the treatment group as compared to haloperidol and sulpiride. Results: There was a significant (P<0.05) increase in serum prolactin level and decrease in dopamine level in the haloperidol and sulpiride treated animals. However, methanolic extract of Tinospora cordifolia significantly (P<0.05) decreased serum prolactin level and increased brain dopamine level. Further, superoxide dismutase and catalase level were also decreased significantly in the haloperidol and sulpiride treated groups as compared to those of the control group and the antioxidant status was restored significantly on treatment with methanolic extract of Tinospora cordifolia. Furthermore, methanolic extract of Tinospora cordifolia also reduced total leukocyte count, and increased red blood cell count and hemoglobin concentration. In addition, the spleen did not show signs of infection or inflammation in the experiments. Conclusions: Methanolic extract of Tinospora cordifolia has a significant anti-hyperprolactinemic effect which may be attributed to neuroprotective and antioxidant effects of its signature constituents like stepharanine. 展开更多
关键词 ANTIPSYCHOTIC NEUROLEPTIC Dopamine PROLACTIN Superoxide DISMUTASE CATALASE
Binding studies of trans-resveratrol with superoxide dismutase (SOD1): Docking assessment and Thermoanalysis 预览
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作者 Janhvi Dureja Renu Chadha +2 位作者 Maninder Karan Akshita Jindal Kunal Chadha 《制药与生物制药研究杂志(英文)》 2019年第1期21-27,共7页
The binding pursuits of trans-resveratrol(t-RSV),an amazing health supplement are investigated with an antioxidant enzyme,superoxide dismutase(SOD1).The aim of the study is to dock t-RSV on the adrenaline binding site... The binding pursuits of trans-resveratrol(t-RSV),an amazing health supplement are investigated with an antioxidant enzyme,superoxide dismutase(SOD1).The aim of the study is to dock t-RSV on the adrenaline binding site on SOD1 in order to explore its potential to act as a safety net against amyotrophic lateral sclerosis(ALS),a fatal neurodegenerative disorder that affects motor neurons.In silico GLIDE docking methodology and in vitro microcalorimetry technique is utilized for the investigation of binding parameters of t-RSV with SOD1.The study provides useful and distinct information about the amino acids involved in the interactions at molecular level along with the nature of forces involved in binding of t-RSV with SOD1.The docking analysis using the scoring functions of Schrodinger’s Glide package depicts that GLU100,PRO28,LYS23,TRP32 residues of the peptide backbone on SOD1 interact with phenolic groups of t-RSV.The information on thermodynamic parameters,i.e.binding constant(Kb),free energy(△G)and enthalpy(△H)generated through calorimetric titrations suggests that the reaction between t-RSV and SOD1 is spontaneous and exothermic.Both the studies are found to be in close agreement with each other based as far as the magnitude of binding constant(Kb=9.9×10^4)is concerned. 展开更多
关键词 TRANS-RESVERATROL superoxide DISMUTASE DOCKING MICROCALORIMETRY binding constant free energy
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姜黄素对心肌细胞缺血再灌注损伤的作用及机制研究 预览 被引量:1
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作者 孙萌 徐琨 +4 位作者 郭敏 史红涛 韩清华 贾永平 王睿 《中西医结合心脑血管病杂志》 2018年第2期147-152,共6页
目的 观察姜黄素(Cur)对心肌细胞缺血再灌注(IR)损伤的保护作用并探讨其可能机制.方法 通过培养H9C2 心肌细胞进行体外模拟心肌细胞缺血再灌注实验.观察Cur 预处理是否减轻IR 损伤心肌细胞,按随机区组法分为对照组、IR 组和Cur(2.5... 目的 观察姜黄素(Cur)对心肌细胞缺血再灌注(IR)损伤的保护作用并探讨其可能机制.方法 通过培养H9C2 心肌细胞进行体外模拟心肌细胞缺血再灌注实验.观察Cur 预处理是否减轻IR 损伤心肌细胞,按随机区组法分为对照组、IR 组和Cur(2.5μmol,5μmol、10μmol)+IR 组.采用MTT 法检测不同浓度Cur 预处理SIRT3 对IR 损伤心肌细胞的保护作用,按随机区组法分为IR 组、Cur+IR 组、Cur+3 TYP(SIRT3 抑制剂)+IR 组、3 TYP+IR 组.TUNEL 法检测心肌细胞凋亡,Western blotting 法检测心肌细胞沉默信息调节因子(SIRT3)、AcSOD、BCl 2、BAX 表达水平,氧化应激试剂盒检测超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH Px)和丙二醛(MDA)氧化应激指标.结果 与IR 组比较,Cur 组(2.5μmol、5 μmol、10 μmol)预处理显著增加细胞存活率(P 〈0.01);不同浓度Cur 组组间比较,Cur(5μmol、10μmol)组与Cur(2.5μmol)组比较显著增加细胞活力(P 〈0.01).与对照组和IR组比较,Cur 预处理显著降低心肌细胞凋亡指数(P 〈 0.01).与IR组比较,Cur + IR组和Cur + 3 TYP+ IR组的SOD和GSH Px 活性显著升高(P 〈0.01),Cur+IR 组MDA 水平显著降低(P 〈0.01);与Cur+IR 组比较,Cur+3 TYP+IR 组和3 TYP+IR 组MDA 的水平显著增加(P 〈0.01).与IR 组比较,Cur+IR 组心肌细胞BCl 2、SIRT3 表达水平显著增加(P 〈0.01),Bax 和AcSOD 表达水平显著降低(P 〈0.01);与3-TYP 预处理组比较,Cur 预处理对SIRT3 和乙酰化SOD 的表达水平逆转(P 〈0.01).结论 Cur 可保护心肌细胞缺血再灌注损伤,其机制可能与Cur 通过上调AcSOD 水平促进SIRT3 表达而发挥抗损伤的保护作用. 展开更多
关键词 缺血再灌注 姜黄素 沉默信息调节因子3 心肌细胞 超氧化物歧化酶 谷胱甘肽过氧化物酶 丙二醛
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Visible light-driven superoxide generation by conjugated polymers for organic synthesis
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作者 Feili Lai Yue Wang +5 位作者 Dandan Li Xianshun Sun Juan Peng Xiaodong Zhang Yupeng Tian Tianxi Liu 《纳米研究:英文版》 SCIE EI CAS CSCD 2018年第2期1099-1108,共10页
得益于他们的唯一的去除的电子结构,结合的基于聚合物的半导体广泛地在器官的电子学,传感器,和生物医学的应用的地里被使用。然而,因为,结合的聚合物的 photocatalytic 性质很少被学习了他们的不合适乐队结构。此处,我们有创作力... 得益于他们的唯一的去除的电子结构,结合的基于聚合物的半导体广泛地在器官的电子学,传感器,和生物医学的应用的地里被使用。然而,因为,结合的聚合物的 photocatalytic 性质很少被学习了他们的不合适乐队结构。此处,我们有创作力地证明乐队结合的聚合物组织,提供为有悦耳的轻吸收性质的没有金属的光催化剂的设计的有效策略是强烈与他们聚合(DP ) 的度有关。poly 拿(3- hexylthiophene )(PHT ) 作为一个例子,我们证明有合适的 DP 的 PHT nanofibers 是新奇可见光驱动的光催化剂,它能乐意地把分子的氧变换成 superoxide 离子。得益于产生 superoxides 的高选择, PHT nanofibers 显示为进 imines 与的胺的氧气的氧化的突出的活动将近百分之百变换和选择。这研究为先进结合的基于聚合物的光催化剂的设计提供新策略。 展开更多
关键词 聚合物 光驱动 器官 合成 电子结构 光催化剂 吸收性质 生物医学
苏木对糖尿病大血管病变中SOD、TNF-α、MCP-1的影响
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作者 刘影哲 温宇婷 +3 位作者 闫博馨 丁家喻 王雪 潘祥宾 《现代生物医学进展》 CAS 2018年第14期2637-2640,共4页
目的:探讨苏木对糖尿病大血管病变大鼠血清超氧化物歧化酶(SOD)、肿瘤坏死因子α(TNF-α)、单核细胞趋化因子1(MCP-1)水平的影响。方法:将24只雄性SD大鼠随机分为4组,每组6只,分别为对照组、模型组、α-硫辛酸组和苏木组。除去... 目的:探讨苏木对糖尿病大血管病变大鼠血清超氧化物歧化酶(SOD)、肿瘤坏死因子α(TNF-α)、单核细胞趋化因子1(MCP-1)水平的影响。方法:将24只雄性SD大鼠随机分为4组,每组6只,分别为对照组、模型组、α-硫辛酸组和苏木组。除去对照组,余下的18只大鼠单次腹腔注射STZ50 mg/kg制备糖尿病大鼠模型,对照组予以注射等体积的柠檬酸-柠檬酸钠缓冲液。成功造模后,苏木组给予0.15 g/kg/d的苏木,α-硫辛酸组给予20 mg/kg/d的α-硫辛酸,每日1次给药,连续给药8周。8周末,检测和比较两组大鼠血清中SOD、TNF-α、MCP-1水平的变化。结果:治疗后,苏木组及α-硫辛酸组血清SOD含量均高于模型组(P〈0.05),但苏木组和α-硫辛酸组组间比较无统计学差异(P〉0.05);苏木组及α-硫辛酸组血清TNF-α、MCP-1含量均显著低于模型组(P〈0.05),与对照组没有明显差别(P〉0.05)。结论:苏木可提高糖尿病大血管病大鼠血清SOD含量,降低血清TNF-α、MCP-1含量。 展开更多
关键词 苏木 糖尿病大血管病变 Superoxide DISMUTASE Tumor NECROSIS factor-α MONOCYTE CHEMOATTRACTANT protein-1
Evaluation of in vitro antioxidant, antiglycation and antimicrobial potential of indigenous Myanmar medicinal plants
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作者 The Su Moe Htet Htet Win +3 位作者 Thin Thin Hlaing War War Lwin Zaw Min Htet Khin Mar Mya 《结合医学学报:英文版》 CAS CSCD 2018年第5期358-366,共9页
关键词 抗氧化剂 抗菌剂 植物 药用 PSEUDOMONAS 评估 生土 CANDIDA
The effect of curcumin as an antioxidant on cochlea fibroblasts in ototoxic rat models
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作者 Tengku Siti Hajar Haryuna Agustinus Hamonangan Winston Purba +1 位作者 Farhat Farhat Soehartono Taat Putra 《中国药学:英文版》 CAS CSCD 2018年第12期847-854,共8页
Aminoglycosides (e.g. Gentamicin) are ototoxic drugs and widely prescribed due to their effective antimicrobial actions and affordable prices. This study focused on determining protective effect of curcumin against th... Aminoglycosides (e.g. Gentamicin) are ototoxic drugs and widely prescribed due to their effective antimicrobial actions and affordable prices. This study focused on determining protective effect of curcumin against the damage caused by aminoglycosides. We aimed to demonstrate the potential of curcumin as an antioxidant to increase the expressions of superoxide dismutase (SOD) in fibroblasts of cochlea lateral wall in ototoxic rat models. The experiment was conducted with randomized post test-only control group design by using 32 male Rattusnorvegicus adults which received a combination of gentamicin and curcumin with different durations and doses. Then, the rats underwent terminations and immunohistochemical assay to determine the expression of SOD. The rats receiving gentamicin injection showed significantly decreased expression of SOD (P<0.05), and the administration of curcumin before and after the gentamicin injection showed significantly increased expression of SOD (P<0.05). Collectively, we showed that curcumin was an antioxidant against oxidative stress due to ototoxicity evidenced by the expression of SOD. 展开更多
关键词 CURCUMIN ANTIOXIDANT GENTAMICIN Superoxide DISMUTASE Preventive Rat Experimental
Superoxide dismutase mimetic ability of Mn-doped ZnS QDs
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作者 Xiangling Ren Mingqian Wang +6 位作者 Xing He Zheng Li Jing Zhang Wei Zhang Xudong Chen Hong Ren Xianwei Meng 《中国化学快报:英文版》 SCIE CAS CSCD 2018年第12期1865-1868,共4页
Superoxide dismutase(SOD) is an important antioxidant enzyme in the body. SOD has special physiological activity and is the primary substance for scavenging free radicals in living organisms.However, the expensive and... Superoxide dismutase(SOD) is an important antioxidant enzyme in the body. SOD has special physiological activity and is the primary substance for scavenging free radicals in living organisms.However, the expensive and complex extraction processes, low SOD yield, as well as difficult to store at room temperature have seriously hindered its application pace. Herein, the enzyme mimetic function of Mn-doped ZnS quantum dots(QDs) was discovered. The improved Marklund and McCord method both showed that Mn-doped ZnS QDs possess intrinsic SOD-like activity. The effects of temperature and pH on the mimetic enzyme activity of Mn-doped ZnS QDs have been investigated compared with SOD enzymes.The low cost and easy to synthesize white Mn-doped ZnS QDs with good biocompatibility are expected to be used as a new type of SOD nanozymes in the biology-relevant fields. 展开更多
关键词 MN-DOPED ZNS QDS Superoxide DISMUTASE Nanozymes Marklund method PYROGALLOL
动物血红素过氧化物酶参与细菌氧化Mn(Ⅱ)的研究进展
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作者 郑洁 孟佑婷 刘桂君 《微生物学报》 CSCD 北大核心 2017年第7期969-977,共9页
锰氧化物是自然环境中一种重要的高活性矿物,在多种元素的生物地球化学循环中起着重要作用。细菌对锰氧化物的形成具有推动作用。截至目前,研究者已从环境中分离出多株锰氧化细菌,并在氧化机理的研究上取得了一定的进展。目前细菌中已... 锰氧化物是自然环境中一种重要的高活性矿物,在多种元素的生物地球化学循环中起着重要作用。细菌对锰氧化物的形成具有推动作用。截至目前,研究者已从环境中分离出多株锰氧化细菌,并在氧化机理的研究上取得了一定的进展。目前细菌中已知的锰氧化酶包括多铜氧化酶和动物血红素过氧化物酶。与多铜氧化酶相比,动物血红素过氧化物酶在蛋白结构与氧化方式上都具有自己的特点。本文结合国内外最新研究结果,在氧化菌株、氧化酶和基因、氧化方式及影响因素等方面对动物血红素过氧化物酶参与细菌氧化Mn(Ⅱ)的研究进行了总结,对未来研究方向进行了展望。 展开更多
关键词 锰氧化细菌 锰氧化物 锰氧化酶 动物血红素过氧化物酶 超氧化物
Correlation of serum GP73, SOD and GPC3 contents with cell proliferation and angiogenesis in liver cancer lesion
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作者 Hua Xin 《海南医科大学学报(英文版)》 2017年第22期74-77,共4页
Objective: To study the correlation of serum GP73, SOD and GPC3 contents with cell proliferation and angiogenesis in liver cancer lesion. Methods: Patients who were diagnosed with primary liver cancer in Jianghan Oilf... Objective: To study the correlation of serum GP73, SOD and GPC3 contents with cell proliferation and angiogenesis in liver cancer lesion. Methods: Patients who were diagnosed with primary liver cancer in Jianghan Oilfield General Hospital between June 2014 and February 2017 were selected as liver cancer group, and healthy subjects who received physical examination in Jianghan Oilfield General Hospital during the same period were selected as control group. Serum was collected from two groups of subjects to determine the contents of GP73, SOD and GPC3;liver cancer lesion and adjacent lesion were collected from liver cancer group to determine the expression of cell proliferation molecules and angiogenesis molecules. Results: Serum GP73 and GPC3 levels of liver cancer group were obviously higher than those of control group while SOD content was obviously lower than that of control group;DNMT3B, STC2, SIRT6, LETM1, EphB4, SULT2B1, HIF-1 , VEGF, Ang-2, HGF and TGF-β1 protein expression levels in liver cancer lesion of liver cancer group were significantly higher than those in adjacent lesion;DNMT3B, STC2, SIRT6, LETM1, EphB4, SULT2B1, HIF-1 , VEGF, Ang-2, HGF and TGF-β1 protein expression levels in liver cancer lesion of liver cancer group were positively correlated with serum GP73 and GPC3 levels, and negatively correlated with serum SOD level. Conclusion: The changes of GP73, SOD and GPC3 levels in the serum of patients with liver cancer are closely related to the cell proliferation and angiogenesis in liver cancer lesion. 展开更多
关键词 Primary liver cancer GOLGI protein 73 Superoxide DISMUTASE GLYPICAN 3 Proliferation ANGIOGENESIS
利用电子顺磁共振和紫外/可见吸收光谱研究熔融KNO3-NaNO2-NaNO3盐分解过程中的超氧负离子 预览
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作者 刘舒婷 苏涛 +2 位作者 张鹏 费泽杰 刘洪涛 《化学物理学报》 SCIE CAS CSCD 2017年第4期372-378,I0001共8页
本实验研制了高温原位紫外-可见吸收光谱仪,并结合标准的电子顺磁共振谱仪来研究HTS(53wt%KNO3—40wt%NaNO2-7wt%NaNO3)熔盐分解过程中是否产生超氧离子.实验结果表明NaNO2相对于NaNO3更易于产生超氧负离子,并且熔盐中的超氧负... 本实验研制了高温原位紫外-可见吸收光谱仪,并结合标准的电子顺磁共振谱仪来研究HTS(53wt%KNO3—40wt%NaNO2-7wt%NaNO3)熔盐分解过程中是否产生超氧离子.实验结果表明NaNO2相对于NaNO3更易于产生超氧负离子,并且熔盐中的超氧负离子在420~440nm有吸收峰,在HTS熔盐中该吸收峰随温度升高发生红移.这个吸收峰可归属为NaO2或KO2电荷转移跃迁,该跃迁将会导致分解后熔盐显黄色.同时实验还获取了高温熔融态的NaNO2和NaNO3紫外可见吸收光谱,并在文中与HTS结果进行详细对比和讨论. 展开更多
关键词 超氧负离子 HTS熔盐分解 高温原位紫外-可见吸收光谱 电子顺磁共振谱
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不同玉米品种对盐胁迫的生理响应 预览 被引量:1
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作者 魏岚岚 《黑龙江农业科学》 2017年第3期27-29,共3页
为了探索外源因素影响玉米耐盐性的机理,以3个玉米品种龙单42、庆单4号和先玉335为材料,研究其在不同浓度盐胁迫下SOD活性、POD活性和脯氨酸含量的变化情况。结果表明:随着处理NaCl溶液浓度的升高,所有品种的这3个指标均表现升高;且在N... 为了探索外源因素影响玉米耐盐性的机理,以3个玉米品种龙单42、庆单4号和先玉335为材料,研究其在不同浓度盐胁迫下SOD活性、POD活性和脯氨酸含量的变化情况。结果表明:随着处理NaCl溶液浓度的升高,所有品种的这3个指标均表现升高;且在NaCl溶液浓度为300mmol·L~(-1)时,达到最高值;先玉335的指标均表现最好,说明其耐盐能力较高。 展开更多
关键词 玉米 苗期 SOD POD 脯氨酸
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荫蔽花生转入自然光照下光合作用的光抑制及光保护机制 预览 被引量:3
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作者 吴正锋 孙学武 +8 位作者 左绍玲 沈浦 刘俊华 万书波 郑永美 于天一 王才斌 陈殿绪 郑亚萍 《中国油料作物学报》 CSCD 北大核心 2017年第5期648-654,共7页
为探明荫蔽花生转入自然光照下的光抑制及光合恢复过程中的光保护机制,为麦套花生高产栽培提供理论依据。大田条件下以花生品种白沙1016为材料,用遮阴网设置不遮光(Control)、遮光50%(S50)和遮光85%(S85)3个处理,研究荫蔽花生转... 为探明荫蔽花生转入自然光照下的光抑制及光合恢复过程中的光保护机制,为麦套花生高产栽培提供理论依据。大田条件下以花生品种白沙1016为材料,用遮阴网设置不遮光(Control)、遮光50%(S50)和遮光85%(S85)3个处理,研究荫蔽花生转入自然光照下光合荧光参数、活性氧离子产生速率及活性氧清除酶活性的变化动态。结果表明,遮阴解除后0-5d花生功能叶片净光合速率(Pn)和光系统Ⅱ最大光化学效率(Fv/Fm值)持续降低,Pn和Fv/Fm值各处理均表现为Control〉遮光50%(S50)〉遮光85%(S85);遮光50%和遮光85%处理净光合速率分别于遮阴解除后10d和20d逐渐恢复到对照水平,Fv/Fm值分别于遮阴解除后12和15d左右恢复对照水平。遮阴解除后0-10d反映热耗散强度的初始荧光F0值、非光化学淬灭系数(NPQ)显著上升,随后逐步降低恢复到对照水平。进一步研究表明,遮阴解除后0-5d超氧阴离子O2-·的产生速率迅速增加,第5d超氧阴离子O2-·产生速率S85〉S50〉Control;而后随SOD、CAT等活性氧清除酶活性的增强,超氧阴离子O2-·的产生速率降低。由以上可看出,遮阴解除后5d光合作用的光抑制或光破坏持续加重,活性氧的产生是导致光破坏的重要原因,热耗散和活性氧的清除在光合恢复中起重要的保护作用。 展开更多
关键词 花生 光抑制 光保护 活性氧 热耗散
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菜青虫感染金龟子绿僵菌后体内几种保护酶活性的变化 预览 被引量:3
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作者 李世广 窦婷婷 +2 位作者 付小伶 刘祝琴 何超 《植物保护》 CAS CSCD 北大核心 2016年第3期133-136,共4页
为了明确菜青虫感染金龟子绿僵菌后体内的防御机制,采用分光光度法测定了菜青虫被金龟子绿僵菌感染后体内保护酶活性的变化。结果表明,用金龟子绿僵菌处理后菜青虫体内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)均... 为了明确菜青虫感染金龟子绿僵菌后体内的防御机制,采用分光光度法测定了菜青虫被金龟子绿僵菌感染后体内保护酶活性的变化。结果表明,用金龟子绿僵菌处理后菜青虫体内超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)均呈现先上升后下降趋势,反映出菜青虫在金龟子绿僵菌侵染初期防御能力增强,后期降低。其中,SOD、POD和CAT活力分别在接种后36、24和48h达到高峰,说明菜青虫在抵抗金龟子绿僵菌侵染过程中,首先发生作用的保护酶是POD,随后为SOD,而CAT是最后发生作用的保护酶。 展开更多
关键词 菜青虫 金龟子绿僵菌 超氧化物歧化酶 过氧化物酶 过氧化氢酶
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