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淫羊藿素对人肺癌A549细胞存活、迁移和侵袭能力的影响及作用机制研究 预览
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作者 林云 李宁 +3 位作者 李艳 银建华 蒋碧佳 卢春兰 《癌症进展》 2019年第15期1763-1765,1770共4页
目的探讨淫羊藿素对人肺癌A549细胞存活、迁移和侵袭能力的影响及作用机制。方法将采用10mmol/L和20mmol/L淫羊藿素处理的人肺腺癌A549细胞,分别作为ICT-L组和ICT-H组,将未接受淫羊藿素处理的细胞作为对照组。采用CCK8法检测细胞存活率... 目的探讨淫羊藿素对人肺癌A549细胞存活、迁移和侵袭能力的影响及作用机制。方法将采用10mmol/L和20mmol/L淫羊藿素处理的人肺腺癌A549细胞,分别作为ICT-L组和ICT-H组,将未接受淫羊藿素处理的细胞作为对照组。采用CCK8法检测细胞存活率,划痕实验检测细胞迁移能力,Transwell小室检测细胞侵袭能力,蛋白质印迹法(Westernblot)检测PIM1蛋白的相对表达量。结果ICT-H组和ICL-L组人肺腺癌A549细胞的存活率、迁移率和侵袭率均明显低于对照组细胞,差异均有统计学意义(P﹤0.01);ICT-H组人肺腺癌A549细胞的存活率、迁移率和侵袭率均明显低于ICL-L组细胞,差异均有统计学意义(P﹤0.01)。ICT-H组和ICL-L组人肺腺癌A549细胞中PIM1蛋白的相对表达量均明显低于对照组细胞,差异均有统计学意义(P﹤0.01);ICT-H组人肺腺癌A549细胞中PIM1蛋白的相对表达量明显低于ICL-L组细胞,差异均有统计学意义(P﹤0.01)。结论淫羊藿素可能通过抑制PIM1蛋白的表达,抑制人肺腺癌A549细胞存活、迁移及侵袭能力。 展开更多
关键词 淫羊藿素 肺腺癌 存活 迁移 侵袭
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因势利导与自生能力:可持续基层治理创新的分析框架 预览
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作者 杨文欢 《吉首大学学报:社会科学版》 CSSCI 北大核心 2019年第4期134-142,共9页
基层治理创新是推进基层治理体系和治理能力现代化的重要内容。在政府因势利导和社会自生能力的分析框架中研究基层治理创新兴衰的发生机制,发现:为做出政绩,采取不符合地区要素禀赋结构的创新战略,引发创新制度的扭曲和社会自生能力的... 基层治理创新是推进基层治理体系和治理能力现代化的重要内容。在政府因势利导和社会自生能力的分析框架中研究基层治理创新兴衰的发生机制,发现:为做出政绩,采取不符合地区要素禀赋结构的创新战略,引发创新制度的扭曲和社会自生能力的弱化,导致基层治理的恶性循环,造成创新的衰退;以人民为中心,采取符合地区要素禀赋结构的创新战略,催生新规则新制度,增强社会自生能力,促进基层治理的良性循环,推动基层治理创新的持续。面向未来的基层治理创新,必须根据未来地区要素禀赋结构和地区发展目标的变化,构建简约高效的创新体系,提高社会自生能力,营造良性创新生态。 展开更多
关键词 因势利导 自生能力 基层治理创新 创新生态
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低氧处理不同时间对人肺腺癌A549细胞增殖的影响及相应缺氧模型探讨
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作者 张璐 沈红 +2 位作者 沈立 朱祎娜 崔进 《现代生物医学进展》 CAS 2019年第1期21-25,共5页
目的:观察低氧处理不同时间对人肺腺癌A549细胞增殖的影响,探讨合理的人肺腺癌细胞株A549体外模拟缺氧时间。方法:将人肺腺癌细胞A549细胞株在低氧环境下分别培养12 h、24 h、48 h、72 h,设置常氧对照组,通过CCK8法测定A549细胞存活率,R... 目的:观察低氧处理不同时间对人肺腺癌A549细胞增殖的影响,探讨合理的人肺腺癌细胞株A549体外模拟缺氧时间。方法:将人肺腺癌细胞A549细胞株在低氧环境下分别培养12 h、24 h、48 h、72 h,设置常氧对照组,通过CCK8法测定A549细胞存活率,RT-PCR和免疫印迹分别检测细胞缺氧诱导因子-1α(hypoxia-inducible factor-1α, HIF-1α)和血管内皮生长因子(vascularendothelial growth factor, VEGF)mRNA及蛋白的表达。结果:低氧24 h组A549细胞存活率最高,低氧48 h、72 h组A549细胞存活率呈时间依赖性明显下降(P<0.001)。自低氧12 h起,A549细胞HIF-1αmRNA和VEGFmRNA的表达开始随低氧时间延长而显著增加(P均<0.001);HIF-1α和VEGF蛋白表达自24 h开始随低氧时间延长而显著增加(P均<0.001)。结论:低氧诱导的A549细胞存活率呈时间依赖性降低,而HIF-1α、VEGF表达呈时间依赖性增高,人肺癌细胞株A549缺氧模型最适时间为24 h。 展开更多
关键词 低氧 活性 缺氧诱导因子-1Α 血管内皮生长因子 模型
The Logic of Chinese Farmer’s Citizenization 预览
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作者 Fu Zhihu Wu Lingwei 《当代社会科学(英文)》 2019年第3期144-155,共12页
Farmers’ citizenization concerns three logical aspects. First, farmers’ viability determines whether they can truly be citizenized and whether they can realize scale management of rural land. Second, farmers’ viabi... Farmers’ citizenization concerns three logical aspects. First, farmers’ viability determines whether they can truly be citizenized and whether they can realize scale management of rural land. Second, farmers’ viability also determines the time for the realization of scale management of rural land and subsequently determines the coming of the Lewis turning point and commercialization point. Third, the key to viability’s coming into play lies in the government’s institutional supply. Citizenization is in step with the long process of scale management of farmland, the coming of the Lewis turning point and commercialization point, the eventual eradication of the impact of the household registration system, and the development of professional farmers. Farmers’ viability is the ultimate key to such synchronized challenges. There are two approaches to the enhancement of farmers’ viability. The first is “to invest in farmers” and the second is to alleviate the existing household registration system’s constraints on farmers. The first approach outweighs the second, for its quintessential essence is to increase farmers’ per capita capital, enhance their viability and transform China from a country with vast human resources into a country with quality human resources. 展开更多
关键词 CITIZENIZATION VIABILITY scale of FARMLAND management the Lewis TURNING POINT and COMMERCIALIZATION POINT household registration system (hukou)
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LncRNA LOC90024对肺癌细胞生长、迁移和侵袭的影响 预览
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作者 李雪萍 梁鑫 +4 位作者 刘政 杨陟华 朱茂祥 周平坤 顾永清 《西安交通大学学报:医学版》 CAS CSCD 北大核心 2019年第1期43-48,共6页
目的探索新长链非编码RNA(LncRNA)LOC90024对肺癌A549细胞生长、迁移和侵袭的影响。方法RT-qPCR检测LOC90024在人肺癌细胞和人正常肺上皮细胞的表达水平。构建LOC90024过表达质粒并在A549细胞内表达,RT-qPCR检测LOC90024表达效果,MTT、... 目的探索新长链非编码RNA(LncRNA)LOC90024对肺癌A549细胞生长、迁移和侵袭的影响。方法RT-qPCR检测LOC90024在人肺癌细胞和人正常肺上皮细胞的表达水平。构建LOC90024过表达质粒并在A549细胞内表达,RT-qPCR检测LOC90024表达效果,MTT、划痕实验和Transwell小室法分别检测LOC90024对A549细胞生长、迁移和侵袭的影响。结果成功构建LOC90024过表达质粒,RT-qPCR结果证实实现过表达;RT-qPCR结果显示LOC90024在肺癌细胞的表达高于人正常肺上皮细胞;MTT检测结果显示在A549细胞中过表达组较空载体组吸光值显著增加;划痕实验结果显示过表达组与空载体组相比,细胞划痕愈合能力明显增加;Transwell小室实验结果显示过表达组细胞穿膜数较空载体组明显增加,A620的吸光度值增高。结论过表达LOC90024可促进肺癌细胞A549的生长、迁移和侵袭能力,提示LncRNALOC90024可能在肺癌发生发展中发挥癌基因的作用,有望成为肺癌治疗的新靶点。 展开更多
关键词 长链非编码RNA LOC90024 肺癌 细胞生长 迁移 侵袭
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Physiological Responses of Contrasting Rice Genotypes to Salt Stress at Reproductive Stage 预览
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作者 Maria Elisa B.GERONA Marjorie P. DEOCAMPO +2 位作者 James A.EGDANE Abdelbagi M.ISMAIL Maribel L. DIONISIO-SESE 《水稻科学:英文版》 CSCD 2019年第4期207-219,I0001共14页
Salinity is a major abiotic stress affecting plant growth and productivity. Considerable genetic variation is present in rice in response to salt stress, with higher sensitivity during early seedling and reproductive ... Salinity is a major abiotic stress affecting plant growth and productivity. Considerable genetic variation is present in rice in response to salt stress, with higher sensitivity during early seedling and reproductive stage. In this study, physiological changes in leaves and developing panicles of rice genotypes (IR686, Sadri, Rc222, CSR28, IR670 and Pokkali) contrasting in salt tolerance at the reproductive stage were evaluated in greenhouse experiment under salt stress. The results showed that IR670 and the tolerant-check Pokkali maintained lower Na^+/K^+ ratio, less reduction in chlorophyll concentration, lower malondialdehyde (MDA) production, higher concentrations of reduced ascorbate (reduced AsA), higher proline accumulation and lower percentage reduction in pollen viability than the salt-sensitive genotypes under salt stress. The higher concentration of reduced AsA suggests an efficient ROS-scavenging system. Physiological measurements and pollen viability analysis revealed that Sadri (moderately tolerant at the seedling stage) is sensitive to salt stress at the flowering stage. The findings will be useful in breeding salt tolerant varieties at both seedling and reproductive stages by selecting appropriate genotypes and phenotypes. 展开更多
关键词 Na^+/K^+ ratio PROLINE reproductive stage rice salinity stress reduced ASCORBATE pollen VIABILITY
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“双一流”建设政策的制度调适、实施逻辑与推进机制 预览
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作者 周志刚 宗晓华 《现代教育管理》 CSSCI 北大核心 2019年第6期11-17,共7页
中国将世界一流大学建设纳入了'国家行动'框架,通过重点建设政策推进世界一流大学建设。作为重点建设政策的延续,'双一流'在遴选机制、拨款机制、动态调整机制方面进行了制度调适,同时,'马太效应'、分层区隔、... 中国将世界一流大学建设纳入了'国家行动'框架,通过重点建设政策推进世界一流大学建设。作为重点建设政策的延续,'双一流'在遴选机制、拨款机制、动态调整机制方面进行了制度调适,同时,'马太效应'、分层区隔、身份固化问题依然存在。'双一流'建设需构建开放竞争的高等教育系统,培养重点建设高校的'自生能力';坚持学科基础,弱化分层区隔,避免'双一流'成为新的院校层级划分方式;完善动态调整机制,破除身份壁垒,摆脱路径依赖。 展开更多
关键词 “双一流” 世界一流大学 重点建设政策 自生能力
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胡桃楸开花物候特性及花粉生活力 预览
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作者 张丽杰 果冲 +4 位作者 秦柏婷 陆秀君 杨雨春 祁永会 沈海龙 《东北林业大学学报》 CAS CSCD 北大核心 2019年第5期4-8,共5页
为了掌握胡桃楸(Juglans mandshurica Maxim)不同交配类型开花结实特性及遗传规律,调控其性别分化,增加胡桃楸果实产量,以结实盛期胡桃楸为研究对象,观察了胡桃楸开花物候特征和花粉形态,并采用染色法和培养基法测定了不同贮藏条件下花... 为了掌握胡桃楸(Juglans mandshurica Maxim)不同交配类型开花结实特性及遗传规律,调控其性别分化,增加胡桃楸果实产量,以结实盛期胡桃楸为研究对象,观察了胡桃楸开花物候特征和花粉形态,并采用染色法和培养基法测定了不同贮藏条件下花粉生活力和萌发情况。结果表明:胡桃楸属于雌雄同株异花异熟物种,大多具有2种交配类型,而且这2种交配类型在种群中是随机分布的;雄先型雄花序与展叶同步,雌先型雌花发育与展叶同步,雌先型比雄先型的雌花期早5~8 d,雄花期晚5~6 d。同一类型树木的雌花可授期与雄花散粉期多不一致,雌雄花期先后有序相遇性很差,且十分稳定,雌雄花数量相差也较多。扫描电镜下胡桃楸花粉形态为扁球形,花粉表面有8~16个萌发孔,表面有纹饰;花粉收集之后立即测定,联苯胺法测定的胡桃楸花粉生活力最高,离体培养基法更适合其测定和观察花粉萌发力。4℃条件下更适合胡桃楸花粉贮藏。 展开更多
关键词 胡桃楸 雌雄异熟 生殖特性 花粉形态 生活力 萌发率
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真核翻译起始因子5A-1基因在宫颈癌发生中的作用及机制研究 预览
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作者 刘柱 陈文 +3 位作者 史德功 卢彦敏 孙建伟 牟晓峰 《实用老年医学》 CAS 2019年第5期441-444,共4页
目的探讨真核翻译起始因子5A-1(eIF5A-1)在宫颈癌细胞发生中的作用及机制。方法以宫颈癌SiHa细胞系、宫颈癌组织及其相应的癌旁正常组织为研究对象,采用免疫印迹法检测组织中eIF5A-1蛋白表达;采用甲基噻唑基四唑(MTT)比色法检测SiHa细... 目的探讨真核翻译起始因子5A-1(eIF5A-1)在宫颈癌细胞发生中的作用及机制。方法以宫颈癌SiHa细胞系、宫颈癌组织及其相应的癌旁正常组织为研究对象,采用免疫印迹法检测组织中eIF5A-1蛋白表达;采用甲基噻唑基四唑(MTT)比色法检测SiHa细胞的活力;采用 3H-胸腺嘧啶核苷酸参入量( 3H-TdR)检测宫颈癌细胞增殖的变化。结果eIF5A-1基因在宫颈癌组织中的表达明显高于其对应的癌旁正常组织( P < 0.01),其中老年组eIF5A-1蛋白在宫颈癌组织中的表达高于非老年组( P < 0.05);与Empty vector组比较,eIF5A-1 vector载体组宫颈癌细胞活力和增殖能力显著增强;与Negative siRNA组比较,eIF5A-1 siRNA组宫颈癌细胞活力和增殖能力显著减弱。结论 eIF5A-1基因在宫颈癌发生中发挥重要作用,其有望成为宫颈癌防治的分子新靶标。 展开更多
关键词 宫颈癌 真核翻译起始因子5A-1 活力 增殖
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Enclosed stigma contributes to higher spikelet fertility for rice(Oryza sativa L.) subjected to heat stress 预览
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作者 Chao Wu Kehui Cui +4 位作者 Qiuqian Hu Wencheng Wang Lixiao Nie Jianliang Huang Shaobing Peng 《作物学报:英文版》 CAS CSCD 2019年第3期335-349,共15页
With global warming, rice plants may be subjected to heat stress more regularly during the heatsensitive flowering stage, causing spikelet sterility and grain yield loss.Stigma exsertion is considered to increase poll... With global warming, rice plants may be subjected to heat stress more regularly during the heatsensitive flowering stage, causing spikelet sterility and grain yield loss.Stigma exsertion is considered to increase pollen reception and promote female reproductive success.The aim of this study was to investigate the role of stigma exsertion on spikelet fertility at high temperatures.Five rice cultivars(Liangyoupeijiu, Shanyou 63, Huanghuazhan, Nagina 22, and IR64) with differing degrees of stigma exsertion were cultivated and exposed to high temperature at anthesis.Heat-tolerant cultivars did not always show a high percentage of spikelets with exserted stigmas, and vice versa.Irrespective of the presence of more pollen grains on exserted stigmas, spikelets with exserted stigmas did not show greater spikelet fertility than spikelets with fewer exserted stigmas or hidden stigmas under heat stress.GA3 application augmented the percentage of spikelets with exserted stigmas;however, it did not increase spikelet fertility under heat stress.Spikelet fertility of whole panicles was negatively correlated with the percentage of spikelets with exserted stigmas, but positively with that with hidden stigmas.Viability of the hidden stigmas was less reduced than that of exserted stigmas under heat stress, suggesting that hidden stigmas have an advantage in maintaining viability.Heat stress delayed anther dehiscence and reduced the viabilities of both exserted stigmas and pollens, thereby causing low spikelet fertility.Together, these results suggest that high spikelet fertility does not depend on stigma exsertion and that enclosed stigma generally contributes to higher spikelet fertility and heat tolerance under high-temperature conditions during flowering in rice. 展开更多
关键词 Heat stress RICE (Oryza sativa L.) SPIKELET FERTILITY STIGMA exsertion STIGMA and POLLEN VIABILITY
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TTC法快速测定芍药种子生活力 预览
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作者 雷慧霞 于营 +5 位作者 刘亚苓 张新静 邢丽伟 鲁海坤 吴健勇 郭靖 《种子》 北大核心 2019年第3期41-44,共4页
采用2,3,5-氯化三苯基四氮唑染色法(TTC法)测定芍药种子生活力,利用正交试验设计研究浸种温度、浸种时间、TTC浓度、染色温度和染色时间对芍药种子染色效果的影响。结果表明,TTC法能快速测定芍药种子的生活力,其染色最佳条件为30℃浸种1... 采用2,3,5-氯化三苯基四氮唑染色法(TTC法)测定芍药种子生活力,利用正交试验设计研究浸种温度、浸种时间、TTC浓度、染色温度和染色时间对芍药种子染色效果的影响。结果表明,TTC法能快速测定芍药种子的生活力,其染色最佳条件为30℃浸种12 h,30℃条件下0.3%TTC溶液避光染色12 h。 展开更多
关键词 芍药 种子 TTC染色法 生活力
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Systematic study of Nd3+ on structural properties of ZnO nanocomposite for biomedical applications;in-vitro biocompatibility,bioactivity, photoluminescence and antioxidant properties
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作者 Saeed Jafarirad Maryam Salmasi +1 位作者 Baharak Divband Mohammadhassan Sarabchi 《中国稀土学报:英文版》 SCIE EI CAS CSCD 2019年第5期508-514,共7页
Owing to the inconformity in ionic radius between Nd3+ and Zn2+, the successful incorporation of Nd3+ ion into the ZnO nanocrystals still remains a great challenge. In the present study various doping ratios containin... Owing to the inconformity in ionic radius between Nd3+ and Zn2+, the successful incorporation of Nd3+ ion into the ZnO nanocrystals still remains a great challenge. In the present study various doping ratios containing 1 wt%, 5 wt%, 7 wt% and 10 wt% of Nd3+ doped ZnO nanoparticles(Nd/ZnO NPs) were synthesized in which a bio-layer caped the NPs. SEM/EDX analysis was performed on the ZnO and Nd/ZnO NPs. In addition, the as-synthesized NPs were characterized using X-ray diffraction(XRD), dynamic light scattering(DLS), differential reflectance spectroscopy(DRS) and photoluminescence(PL) spectroscopy.The average size of Nd(5 wt%)/ZnO NPs was in the range of 6.22 and 15 e18 nm based on XRD and SEM techniques, respectively. The measured band gap values for pure ZnO and Nd/ZnO NCs with doping ratios of 1 wt%, 5 wt%, 7 wt% and 9 wt% were equal to 3.46, 3.26, 3.05, 3.25 and 3.29, respectively. After inhalation, nanoparticles first interact with lung surfactant system and accordingly their toxic effects will appear on lungs cells such as A549 cell line. The effect of Nd/ZnO NPs to interact by human A549 cell line was evaluated by means of cell viability test. According to cell viability test the concentrations of 0.3 and 0.5 mg/mL of Nd/ZnO NPs induce a low toxicity. The present study shows that these toxic effects of Nd/ZnO NPs can be rectified by capping its surface via the addition of a bio-layer around particles in order to prevent them from interacting A549 cell line. 展开更多
关键词 NEODYMIUM Rare earth elements Cell VIABILITY test NANOSTRUCTURES
Left Ventricular Dysfunction in Ischemic Heart Disease 预览
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作者 Robert F.Hamburger 《心血管创新与应用》 2019年第B01期297-303,共7页
Ischemic heart disease is a growing cause of heart failure owing to the increasing prevalence of diabetes,hypertension,and obesity.It is important to understand the concepts of myocardial stunning and hibernation so w... Ischemic heart disease is a growing cause of heart failure owing to the increasing prevalence of diabetes,hypertension,and obesity.It is important to understand the concepts of myocardial stunning and hibernation so we as physicians can order appropriate testing on patients with ischemic heart disease,such as myocardial viability studies.Ventricular remodeling is associated with an elevated risk of death after myocardial infarction.The extent of myocardial viability determines the clinical outcome of patients after myocardial infarction.Multiple impactful clinical trials have changed the way we manage ischemic cardiomyopathy over the past few decades,and medications such as angiotensin-converting enzyme inhibitors,angiotensin II receptor blockers,beta-blockers,and aldosterone antagonists have improved outcomes in this patient population.Similarly,much has been reported on the effect of coronary artery bypass surgery on outcomes in patients with ischemic heart disease and heart failure,but further research studies are needed on the role of percutaneous coronary intervention. 展开更多
关键词 ISCHEMIC HEART DISEASE ISCHEMIC CARDIOMYOPATHY HEART FAILURE VIABILITY
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高光谱技术无损检测单粒小麦种子生活力的特征波段筛选方法研究 预览
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作者 张婷婷 向莹莹 +2 位作者 杨丽明 王建华 孙群 《光谱学与光谱分析》 SCIE EI CAS CSCD 北大核心 2019年第5期1556-1562,共7页
种子活力是种子质量的一项重要指标,高活力的种子具有较强的抗逆性、生长优势及生产潜力。而种子活力在种子生理成熟时最高,随后随着贮藏时间的延长而发生着自然不可逆的降低。因此,在播种前及时、准确地对种子活力进行检测和筛选具有... 种子活力是种子质量的一项重要指标,高活力的种子具有较强的抗逆性、生长优势及生产潜力。而种子活力在种子生理成熟时最高,随后随着贮藏时间的延长而发生着自然不可逆的降低。因此,在播种前及时、准确地对种子活力进行检测和筛选具有重要的实践意义。针对传统种子活力检测方法存在的操作过程复杂繁琐、耗时长、重复性差且对种子有破坏性等缺点,研究尝试利用高光谱成像技术建立单粒小麦种子生活力快速、无损、精确的检测方法。以高温高湿老化后的190粒小麦种子(发芽128粒,不发芽62粒)作为研究样本,先利用可见-近红外(Vis-NIR)高光谱成像系统采集样本种子的光谱图像和进行标准发芽试验,并确保光谱采集试验和标准发芽试验的小麦种子一一对应。随后提取种子光谱图像的感兴趣区域并对其光谱数据进行平均和特征分析。分别采用一阶导数(FD)、均值中心化(MC)、正交信号校正(OSC)和多元散射校正(MSC)对原始光谱数据进行预处理,结合偏最小二乘辨别分析(PLS-DA)建立全波段PLS-DA模型,比较分析,并筛选出最适预处理方法。分别利用无信息变量消除算法(UVE)、竞争性自适应重加权算法(CARS)、连续投影算法(SPA)及耦合不同变量筛选方法对特征波段进行筛选提取,再分别基于所提取出的特征波段建立PLS-DA定性判别模型,对比分析,最终确立提取与单粒小麦种子生活力相关性最高的高光谱特征波段方法体系。结果表明:不同光谱预处理建立的模型其表现有所差异,在MC,FD,OSC和MSC中,采用MC对原始高光谱数据进行预处理,建立的全波段MC-PLS-DA判别模型,其校正集和预测集对小麦种子生活力的整体鉴别正确率分别为82.5%和83.0%,优于原始及其他预处理后建立的全波段PLS-DA判别模型,其校正集和预测集对小麦种子活种子鉴别正确率分别为94.8%和90.6%。进一步对比 展开更多
关键词 高光谱技术 小麦种子 生活力 检测 特征波段
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中西医结合康复护理在脑卒中患者恢复期的干预效果研究
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作者 林海红 胡剑华 《中华全科医学》 2019年第3期515-517,520共4页
目的探究在脑卒中患者恢复期间实行中西医结合康复护理的干预效果。方法选取2016年3月—2018年3月收治在舟山市中医院的脑卒中患者80例,采用数字随机法分为常规护理组和结合护理组,每组40例。常规护理组患者采用常规康复护理,结合护理... 目的探究在脑卒中患者恢复期间实行中西医结合康复护理的干预效果。方法选取2016年3月—2018年3月收治在舟山市中医院的脑卒中患者80例,采用数字随机法分为常规护理组和结合护理组,每组40例。常规护理组患者采用常规康复护理,结合护理组患者采用中西医结合康复护理进行干预。比较2组患者护理干预后的总有效率,统计患者的临床指标、神经功能缺损程度、日常运动功能和生活能力、干预后的生活质量以及复发率和护理满意度。结果结合护理组患者护理干预后的总有效率高于常规护理组(P<0.05);结合护理组患者床上开始活动时间、下床活动时间和住院时间均短于常规护理组患者(均P<0.05);结合护理组患者干预后的CSS评分、Barthel指数和MAS评分均优于常规护理组患者护理后(均P<0.05);结合护理组患者护理干预后的生活质量各项目评分均高于常规护理组(均P<0.05);结合护理组患者护理干预后的复发率低于常规护理组,护理满意度高于常规护理组(均P<0.05)。结论中西医结合康复护理能够缩短脑卒中患者的恢复时间,改善患者的运动功能和日常生活能力,提高其生活质量,且复发率低,安全性高。 展开更多
关键词 中西医康复护理 脑卒中 生活能力 生活质量
蛋白酶体抑制剂MG132对急性髓系白血病细胞增殖与凋亡的影响 预览
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作者 郑晓辉 黄家福 +6 位作者 徐淑娟 李鑫 许雅苹 房纯正 翁乐斌 汪洁 黄黎月 《中国药理学通报》 CAS CSCD 北大核心 2019年第3期327-334,共8页
目的探讨蛋白酶体抑制剂MG132对急性髓系白血病(acutemyeloidleukemia,AML)细胞增殖与凋亡的影响。方法qRT-PCR检测9株血液肿瘤细胞株内ADRM1mRNA表达。利用shRNA干扰HL60细胞内ADRM1表达,将不同浓度的MG132作用于ADRM1干扰前后的HL60细... 目的探讨蛋白酶体抑制剂MG132对急性髓系白血病(acutemyeloidleukemia,AML)细胞增殖与凋亡的影响。方法qRT-PCR检测9株血液肿瘤细胞株内ADRM1mRNA表达。利用shRNA干扰HL60细胞内ADRM1表达,将不同浓度的MG132作用于ADRM1干扰前后的HL60细胞24h,CCK-8法检测各组细胞增殖与活力;Westernblot检测各组细胞内ADRM1、UCH37蛋白表达;流式细胞仪分析不同浓度MG132作用下,HL60、NB4细胞凋亡情况。结果血液肿瘤细胞株内ADRM1mRNA表达上调。成功构建ADRM1shRNA与scrambledshRNAHL60细胞株。ADRM1基因干扰及给予MG132后,各实验组细胞增殖抑制,活力下降,此时细胞内ADRM1、UCH37蛋白表达下调。随着MG132浓度提高,HL60、NB4细胞凋亡增加;MG132对HL60细胞的促凋亡作用强于NB4细胞。结论血液肿瘤细胞株内ADRM1mRNA过表达;ADRM1蛋白下调后,UCH37蛋白亦下调,细胞增殖抑制;MG132通过下调ADRM1、UCH37蛋白表达,诱导AML细胞凋亡,抑制细胞增殖与活力;MG132对不同分型AML细胞的促凋亡作用存在个体差异。 展开更多
关键词 ADRM1 MG132 急性髓系白血病细胞 细胞增殖 活力 凋亡
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Effects of Seed Viability and Number of Generations on Genetic Integrity of Soybean Germplasm Zhonghuang 18 by AFLP Markers 预览
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作者 Dong WANG Xiaodong ZHANG +7 位作者 Runfang LI Lingyun LU Xiaomu WANG Xiaohong GU Xia XIN Guangkun YIN Xinxiong LU Hanfeng DING 《农业生物技术:英文版》 CAS 2019年第2期10-17,共8页
The seeds of a soybean cultivar Zhonghuang 18 were subjected to accelerated aging for 0 (population G 0-1), 112 (population G 0-2), 154 (population G 0-3) and 196 d (population G 0-4), whose germination percentage was... The seeds of a soybean cultivar Zhonghuang 18 were subjected to accelerated aging for 0 (population G 0-1), 112 (population G 0-2), 154 (population G 0-3) and 196 d (population G 0-4), whose germination percentage was found to be 98.0%, 95.0%, 81.0%, and 79.0%, respectively. Then, the four populations were regenerated twice in the field. The first descendant populations were marked as G 1-1, G 1-2, G 1-3 and G 1-4, and the second were marked as G 2-1, G 2-2, G 2-3 and G 2-4, respectively. The genetic variation between the control population (G 0-1) and the experimental populations was analyzed using 12 AFLP primer combinations. The results showed that there was no significant difference in genetic similarity between the 11 experimental populations and the control population G 0-1. The genetic similarity between population G 2-4 and G 0-1 was still as high as 0.933 3, indicating that the F 2 generation of the population whose germination percentage was only 79.0% still had a high genetic similarity to the control population. The results of t -tests revealed that the populations G 1-1, G 2-1, G 1-2 and G 2-2 showed no significant difference from the control population G 0-1 in effective number of alleles per locus (Ae), genetic diversity index (H) and Shannon s diversity index (I), while these indices of populations G 0-3, G 0-4, G 1-3, G 1-4, G 2-3 and G 2-4 were significantly reduced.χ 2 tests indicated that the populations G 1-1 and G 2-1 showed little difference, and the populations G 0-2, G 0-3, G 0-4, G 1-2, G 1-3, G 1-4, G 2-2, G 2-3, and G 2-4 showed great difference in allele frequency distribution from the control population G 0-1, and the difference was greater when the seed viability was lower. Compared with the control population G 0-1, the number of rare alleles (Nr) of the populations G 0-2, G 1-1, G 2-1, G 1-2 and G 2-2 showed no significant difference, while that of the populations G 0-3, G 0-4, G 1-3, G 1-4, G 2-3 and G 2-4 declined obviously. These results revealed that compared with the control 展开更多
关键词 SOYBEAN Seed VIABILITY NUMBER of generations AFLP Genetic INTEGRITY
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贮藏温度和贮藏时间对垂柳种子生活力的影响 预览
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作者 曹帮华 史峻峰 +3 位作者 张鑫 李业宇 祁琳 秦光华 《山东林业科技》 2019年第1期9-12,共4页
以成熟的垂柳种子为材料,测定了不同贮藏温度和不同贮藏时间下垂柳种子发芽率、发芽势、活力指数等生活力指标的变化,以探索贮藏温度和贮藏时间对垂柳种子生活力的影响。结果表明:适当的低温-10℃、-5℃、4℃可以延缓垂柳种子的劣变,延... 以成熟的垂柳种子为材料,测定了不同贮藏温度和不同贮藏时间下垂柳种子发芽率、发芽势、活力指数等生活力指标的变化,以探索贮藏温度和贮藏时间对垂柳种子生活力的影响。结果表明:适当的低温-10℃、-5℃、4℃可以延缓垂柳种子的劣变,延长其贮藏时间;常温20℃与极低温-20℃条件下会降低种子的生活力,表现为活力指数降低;随贮藏天数的增加,发芽率、发芽势、发芽指数表现出先升高后降低的趋势,发芽速则是先降低后升高。常温20℃贮藏7天种子发芽率显著下降,在-5℃贮藏3天的条件下种子生活力最强,可应用于垂柳种子萌发及人工种苗培育的实践中。 展开更多
关键词 垂柳种子 贮藏温度 贮藏时间 生活力 发芽
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金娃娃萱草花粉的贮藏以及生活力的测定 预览
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作者 宋立功 孙晓光 《河北林业科技》 2019年第1期21-24,共4页
以金娃娃萱草(Hemerocallis fulva‘Golden Doll’)花粉作为试验材料,利用培养基发芽法对花粉生活力进行了测定,筛选出了花粉的适宜染色剂和染色时间,并且研究了不同贮藏条件及不同蔗糖浓度对花粉生活力的影响。结果表明:适宜的染色剂为... 以金娃娃萱草(Hemerocallis fulva‘Golden Doll’)花粉作为试验材料,利用培养基发芽法对花粉生活力进行了测定,筛选出了花粉的适宜染色剂和染色时间,并且研究了不同贮藏条件及不同蔗糖浓度对花粉生活力的影响。结果表明:适宜的染色剂为TTC,染色时间为35~40min。不同贮藏条件下,金娃娃萱草花粉生活力有很大差异,其中以低温干燥贮藏法对保持金娃娃萱草花粉生活力的效果最好。金娃娃萱草花粉萌发的适宜液体培养基蔗糖浓度为150g/L。 展开更多
关键词 金娃娃萱草 花粉 贮藏 生活力
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Scutellarin protects human cardiac microvascular endothelial cells with hypoxia-reoxygenation injury via JAK2/STAT3 signal pathway
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作者 Chen Chen Zhi-ying Weng +7 位作者 You-lan Wang Chang-bo Zheng Yang Li Jian Yang Ze-lan Dai Bai-xi Ji Chuang Xiao Wei-min Yang 《中草药:英文版》 CAS 2019年第1期103-107,共5页
Objective: To investigate the antagonistic cell injury effect and molecular mechanism of scutellarin(SCU)in hypoxia reoxygenation(HR) treated human cardiac microvascular endothelial cells(HCMECs).Methods: The method o... Objective: To investigate the antagonistic cell injury effect and molecular mechanism of scutellarin(SCU)in hypoxia reoxygenation(HR) treated human cardiac microvascular endothelial cells(HCMECs).Methods: The method of 12 h hypoxia following by 12 h reoxygenation was used to culture HCMECs in vitro to built cell injury model. The groups were divided into control group, model(HR) group, and HR + SCU(0.1 μmol/L, 1 μmol/L, and 10 μmol/L) group. The cell viability was determined by MTT, and oxidative stress was detected by malondialdehyde(MDA) levels by biochemical assay kit. Protein expression of JAK2/p-JAK2 and STAT3/p-STAT3 were evaluated by Western blot.Results: The results of MTT and MDA showed that HR decreased the cell viability(P < 0.05) and increased MDA level significantly(P < 0.05), SCU played a contrary role in these processes. Western blot analysis indicates that, the expression of JAK2 and p-JAK2, STAT3, and p-STAT3 were increased in model group when compared with control group(P < 0.05);Compared with model group, their expression were reduced by SCU(P < 0.05).Conclusion: SCU took a protective effect on HR-treated HCMECs, and the molecular mechanism may be associated with the inhibition of JAK2/STAT3 signal transduction pathway. 展开更多
关键词 cell VIABILITY HCMECs hypoxia REOXYGENATION JAK2-STAT3 pathway MDA SCUTELLARIN
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