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Treatment of high-grade dysplasia and intramucosal carcinoma using radiofrequency ablation or endoscopic mucosal resection + radiofrequency ablation: Meta-analysis and systematic review 预览
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作者 Mileine Valente de Matos Alberto Machado da Ponte-Neto +7 位作者 Diogo Turiani Hourneaux de Moura Ethan Dwane Maahs Dalton Marques Chaves Elisa Ryoka Baba Edson Ide Rubens Sallum Wanderley Marques Bernardo Eduardo Guimaraes Hourneaux de Moura 《世界胃肠内镜杂志:英文版(电子版)》 2019年第3期239-248,共10页
BACKGROUND The progression of Barrett’s esophagus(BE) to early esophageal carcinoma occurs sequentially;the metaplastic epithelium develops from a low-grade dysplasia to a high-grade dysplasia(HGD), resulting in earl... BACKGROUND The progression of Barrett’s esophagus(BE) to early esophageal carcinoma occurs sequentially;the metaplastic epithelium develops from a low-grade dysplasia to a high-grade dysplasia(HGD), resulting in early esophageal carcinoma and,eventually, invasive carcinoma. Endoscopic approaches including resection and ablation can be used in the treatment of this condition.AIM To compare the effectiveness of radiofrequency ablation(RFA) vs endoscopic mucosal resection(EMR) + RFA in the endoscopic treatment of HGD and intramucosal carcinoma.METHODS In accordance with PRISMA guidelines, this systematic review included studies comparing the two endoscopic techniques(EMR + RFA and RFA alone) in the treatment of HGD and intramucosal carcinoma in patients with BE. Our analysis included studies involving adult patients of any age with BE with HGD or intramucosal carcinoma. The studies compared RFA and EMR + RFA methods were included regardless of randomization status.RESULTS The seven studies included in this review represent a total of 1950 patients, with742 in the EMR + RFA group and 1208 in the RFA alone group. The use of EMR +RFA was significantly more effective in the treatment of HGD [RD 0.35(0.15,0.56)] than was the use of RFA alone. The evaluated complications(stenosis,bleeding, and thoracic pain) were not significantly different between the two groups.CONCLUSION Endoscopic resection in combination with RFA is a safe and effective method in the treatment of HGD and intramucosal carcinoma, with higher rates of remission and no significant differences in complication rates when compared to the use of RFA alone. 展开更多
关键词 BARRETT ESOPHAGUS RADIOFREQUENCY Endoscopic MUCOSAL resection HALO system
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Identification and Characterization of Genes Responsible for Drought Tolerance in Rice Mediated by Pseudomonas fluorescens 预览
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作者 Manjesh SAAKRE Thirthikar Meera BABURAO +4 位作者 Abida Puthenpeedikal SALIM Rose Mary FFANCIES Valasala Poothecty ACHUTHAN George THOMAS Sajeevan Radha SIVARAJAN 《水稻科学:英文版》 CSCD 2017年第5期291-298,共8页
Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential. Structural and functional characterization of drought stress-induced genes has contributed to a bett... Drought is one of the major abiotic stresses which adversely affect crop plants limiting growth and yield potential. Structural and functional characterization of drought stress-induced genes has contributed to a better understanding of how plants respond and adapt to the drought stress. In the present study, differential display technique was employed to study the gene expression of rice plants at the reproductive stage that were subjected to drought stress by withholding water, Pseudomonas fluorescens strain (Pf1) treated plants subjected for drought stress by withholding water and control (well-watered). Differentially expressed cDNAs of six genes (COX1, PKDP, bZIP1, AP2-EREBP, Hsp20 and COC1) were identified, cloned and sequenced. Real-time qPCR analysis showed that all the six genes were upregulated in drought-stressed plants treated with Pf1. This revealed that the remarkable influence of Pf1 colonization leads to drought tolerance at the reproductive stage. These results showed that high levels of gene expression in plants lacking adequate water can be remarkably influenced by Pf1 colonization, which might be a key element for induced systemic tolerance by microbes. 展开更多
关键词 RICE drought tolerance PSEUDOMONAS FLUORESCENS differential display reverse transcription polymerase chain reaction quantitative real-time PCR TRANSCRIPT derived FRAGMENT
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