期刊文献+

反相高效液相色谱法测定小鼠血清及脏器中白藜芦醇浓度 认领 被引量:6

Determination of Resveratrol in Serum and Organs of Mice by RP - HPLC
在线阅读 免费下载
收藏 分享 导出
摘要 目的:建立以反相高效液相色谱(RP—HPLC)法快速测定小鼠血清及部分脏器匀浆中白藜芦醇浓度的方法。方法:分析柱为Kromasil C18,流动相为甲醇-水(48:52),流速为1ml/min,柱温为室温,柱压为12kPa,检测波长为303nm,进样量为20μl。结果:白藜芦醇检测浓度线性范围为0.25~25μg/ml,最低检测浓度为0.1μg/ml(S/N-3);各样品平均绝对回收率大于88%,方法回收率为96%~100%,相对标准差小于10%。结论:本方法经济、可靠、简单、快速,可为白藜芦醇血药浓度分析及动物体内分布研究提供参考。 OBJECTIVE: To develop RP - HPLC method for the rapid determination of resveratrol in serum and tissue homogenates (in some organs) of mice. METHODS:HPLC determination was performed using Kromasil C18 column,the mobile phase consisted of methanol - water (48 : 52) with a flow rate of lml/min,the column was under room temperature with its pressure at 12kPa, the detection wavelength was 303nm and the sample size was 20μl. RESULTS: The linear range for resveratrol was 0.25~25μg/ml, with minimal detectable drug concentration at 0.1μg/ml (S/N = 3). The average absolute recovery of the samples was above 88% and the methodological recovery rates of which ranged from 96% to 100%, RSD were below 10%. CONCLUSIONS: The established determination method is economical, reliable, simple and rapid, and suitable for the blood concentration determination of resveratrol and the study of its distribution in animal body.
作者 王莉 周红 张三奇 文爱东 顾宜 连佳芳 王婧雯 WANG Li, ZHOU Hong, ZHANG Sanqi, WEN Aidong, GU Yi, LIAN Jiafang, WANG Jingwen (Dept. of Pharmacy, Xijing Hospital,The Fourth Military Medical University, Xi'an 710032, China)
出处 《中国药房》 CAS CSCD 北大核心 2006年第17期 1297-1299,共3页 China Pharmacy
关键词 反相高效液相色谱法 白藜芦醇 血清 组织匀浆 RP- HPLC Resveratrol Serum Tissue homogenates
  • 相关文献

参考文献5

二级参考文献24

共引文献80

同被引文献51

引证文献6

二级引证文献33

维普数据出版直通车
今日学术
投稿分析
职称考试

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈