期刊文献+

成都某种禽场鸡胚沙门氏菌的检测 认领 被引量:8

Detection of Salmonella in Chicken Embryos in a Breeding Poultry Field of Chengdu
在线阅读 下载PDF
收藏 分享 导出
摘要 对四川省成都市某种禽场的156个死胚进行沙门氏菌的分离鉴定及药物敏感性检测。本试验采用沙门氏菌显色培养基、肠杆菌科生化鉴定管、三糖铁试验、沙门氏菌多价血清和16SrRNA PCR鉴定5种方法对疑似菌株进行鉴定,并用6种毒力岛基因将分离的沙门氏菌进行PCR鉴定。结果显示,沙门氏菌的分离率为15.4%(24/156),其中伤寒沙门氏菌占58.3%(14/24);fimY、invA和mgtC毒力基因的检测率均为100%;本试验分离菌对大部分沙门氏菌临床药物表现出明显的耐药性。 In a poultry farm of Chengdu city, Sichuan province, isolation, identification and drug sensitivity test of Salmonella were conducted from 156 dead embryos. In this experiment,we used Salmonella chromogenic medium, biochemical identification of enterobacteriaceae, trisaccharide iron experiment,Salmonella polyvalent serum and 16S rRNA PCR to identify suspected strains, PCR identification of isolated Salmonella were conducted with 6 kinds of virulence genes. The re- sults concluded that Salmonella separation rate was 15.4 % (24/156), including Salmonella typhi accounted for 58.3% (14/24); Detection rates of fimY, invA and mgtC virulence genes were all 100% ;The isolated Salmonella were resistant to most clinical drugs to Salmonella.
作者 张丽芳 肖桥 罗薇 ZHANG Li-fang, XIAO Qiao, LUO Wei (College of Life Science and Technology , Southwest University for Nationalities, Chengdu 610041, China)
出处 《中国畜牧兽医》 CAS 北大核心 2015年第6期1571-1579,共9页 China Animal Husbandry & Veterinary Medicine
基金 四川省科技厅应用基础研究“丙型副伤寒沙门氏菌感染动物模型的建立及其致病机理的研究”(2013JY0045)
关键词 死胚 分离 鉴定 毒力基因 药物敏感性 dead embryo isolation identification virulence genes drug sensitivity
  • 相关文献

参考文献18

  • 1Fakhr M K,Sherwood J S,Thorsness J,et al. Molecu- lar characterization and antibiotic resistance profiling of Salmonella isolated from retail turkey meat prod- ucts[J]. Food Borne Pathogens and Disease, 2006, 3(4) :366-376. 被引量:1
  • 2Herikstad H, Motarjemi Y, Tauxe R V. Salmonella surveillance:A global survey of public health serotyp- ing[J]. Epidemiology and Infecion, 2002,129 : 1-8. 被引量:1
  • 3Gaul S B,Wedel S,Erdman M M,et aL Use of pulsed- field gel electrophoresis of conserved Xba I fragments for identification of swine Salmonella serotypes[J]. Journal of Clinical Microbiology, 2007,45 (2): 472-476. 被引量:1
  • 4Lukinmaa S, Takkunen E, Siitonen A. Molecular epi- demiology of Clostridium perfringens related to food borne outbreaks of disease in Finland from 1984 to 1999[J]. Applied and Environmental Microbiology, 2002,68 (8) : 3744-3749. 被引量:1
  • 5Xia S, Hendriksen R S,Xie Z,et al. Molecular charac- terization and antimicrobial susceptibility of Salmo-nella isolates from infections in humans in Henan province, China [J ]. Journal of Clinical Microbiolo- gy ,2009,47(2) :401-409. 被引量:1
  • 6张兴晓,沈志强.鸡胚内沙门氏菌的分离与鉴定[J].中国禽业导刊,2000,17(14):22-22. 被引量:1
  • 7图布丹扎布金花,田有明,景建国,刘二满.鸡胚及雏鸡沙门氏菌病的病原分离与鉴定[J].中国兽医杂志,1996,22(10):8-9. 被引量:2
  • 8Kong Q, Yang J, Liu Q, et al. Effect of deletion of genes involved in lipopolysaccharide core and O-anti- gen synthesis on virulence and immunogenicity of Salmonella enterica serovar Typhimurium[J]. Infect Immun,2011,79(10) : 4227-4239. 被引量:1
  • 9Ronholm J, Zhang Z, Cao X, et al. Monoclonal anti- bodies to lipopolysaccharide antigens of Salmonella enterica serotype Typhimurium DT104[J]. Hybrido- ma (Larchmt) ,2011,30(1) :43-52. 被引量:1
  • 10宋榴艳,陈守义,侯水平.一株不典型山夫登堡沙门氏菌的鉴定及其16SrRNA序列分析[J].热带医学杂志,2011,11(6):627-628. 被引量:4

二级参考文献109

共引文献87

同被引文献48

引证文献8

二级引证文献18

维普数据出版直通车
今日学术
投稿分析
职称考试

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部 意见反馈