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山东地区生猪和家禽屠宰环节沙门菌血清型分布 认领 被引量:10

Research on serotype distribution of Salmonella isolated from pig and poultry slaughter processes in Shandong Province
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摘要 目的了解山东地区生猪和家禽屠宰环节沙门菌血清型分布,为进一步开展动物源性产品风险评估提供数据。方法 2014年9-12月从山东省部分屠宰场采集分离的335株沙门菌,应用液态悬浮芯片技术对沙门菌菌体(O)抗原、鞭毛(H)抗原和附加靶蛋白(AT)的基因进行检测,然后运用Salmonella serotyping assay软件分析鉴定血清型。结果 335株沙门菌中299株共分为29个血清型,另有36株未分型,优势血清型主要为德尔卑沙门菌(S.Derby,24.8%,83/335、)和肠炎沙门菌(S.enteritidis,15.2%,51/335)。德尔卑沙门菌、汤卜逊沙门菌(S.Thompson)和阿贡纳沙门菌(S.Agona)在生猪和家禽屠宰场中都有分布;生猪屠宰场以德尔卑沙门菌(39.8%,64/161)为主,其次为鼠伤寒沙门菌(S.typhimurium,19.3%,31/161)和汤卜逊沙门菌(13.7%,22/161);家禽屠宰场中以肠炎沙门菌(28.7%,50/174)、印第安纳沙门菌(S.Indiana,12.1%,21/174)和德尔卑沙门菌(10.9%,19/174)为主。另外,不同地区屠宰场的沙门菌血清型呈差异分布,生猪屠宰场中除鲁中部外,其他地区主要血清型为德尔卑沙门菌;家禽屠宰场中,鲁东和鲁西地区主要是肠炎沙门菌,鲁东北地区以德尔卑沙门菌为主;鲁中部地区分离株以汤卜逊沙门菌为主。结论山东省屠宰场中的沙门菌污染比较严重,且有多种血清型,需加强屠宰环节的致病菌监控,降低动物产品在屠宰环节受沙门菌污染的风险。 Objective To acquire original data of Salmonella serotype distribution during pig and poultry slaughter processes in Shandong Province,and to provide data for the risk assessment of animal products. Methods Salmonella was isolated from slaughterhouses in Shandong Province from September to December 2014. x MAP Salmonella serotyping assay kit was used to detect the genes of somatic O antigen,motility H antigen and additional target( AT) gene.Salmonella serotypes were identified by x MAP Salmonella serotyping assay software throught gene information. Results A total of 299 Salmonella isolates were divided into 29 serotypes and 36 isolates could not be typed. S. Derby( 24. 8%,83 /335) and S. Enteritidis( 15. 2%,51 /335) were the major serotypes in pig and poultry slaughter processes in Shandong Province. S. Derby,S. Thompson and S. Agona were detected both in pig and poultry slaughter processes; S. Derby( 39. 8%,64 /161),S. typhimurium( 19. 3%,31 /161) and S. Thompson( 13. 7%,22 /161) were themajor serotypes in pig slaughter process; S. enteritidis( 28. 7%,50 /174),S. Indiana( 12. 1%,21 /174) and S. Derby( 10. 9%,19 /174) were the major serotypes in poultry slaughter process. Salmonella serotypes varied in various areas of ShandongProvince. In the pig slaughter processes,S. Derby was the major serotype except the middle of Shandong Province; In the poultry slaughterhouses,S. enteritidis was the major serotype in the east and west of Shandong Province,S. Derby was the leading serotype in the northeast; S. Thompson was the most prevalent in the middle of Shandong Province. Conclusion There were various Salmonella serotypes at slaughterhouses in Shandong Province,and slaughter processes supervision and control should be strengthened to decrease the risk of Salmonella contamination in animal products.
作者 赵建梅 王娟 王君玮 盖文燕 颜世敢 黄秀梅 曲志娜 ZHAO Jian-mei, WANG Juan, WANG Jun-wei, GAI Wen-yan, YAN Shi-gan, HUANG Xiu-mei, QU Zhi-na (Department for Safety Supervision of Animal Products, China Animal Health and Epidemiology Center, Laboratory of Quality and Safety Risk Assessment for Livestock and Poultry Products ( Qingdao), Ministry of Agriculture, Shandong Qingdao 266032, China)
出处 《中国食品卫生杂志》 2015年第5期503-508,共6页 Chinese Journal of Food Hygiene
基金 国家农产品质量安全风险评估专项(2014)(GJFP2014007) 山东省科技发展计划(2014GSF120006)
关键词 沙门菌 屠宰 血清型 液态悬浮芯片技术 分布 生猪 家禽 山东 Salmonella slaughter serotype x MAP Salmonella serotyping assay distribution pig poultry Shandong
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